Cloning And Heterologous Expression Of Human Nachrs And A Unique Spider Venom Toxin, Christine M. Vega

Theses and Dissertations

Drug development research has long focused on synthesizing novel therapeutics while overlooking isolation from naturally available sources. Recently, the discovery of the therapeutic effects of highly bioavailable animal venom caused the field of venom research to soar in popularity. Animal venom serves as a natural, sophisticated tool optimized over millions of years by evolution with the ability to bind to ion channels such as nAChRs, non-specific ligand-gated ion channels distributed throughout the human nervous system, with high selectivity and specificity. However, purifying individual venom toxins from crude venom is challenging due to its availability. Therefore, synthesizing large quantities of venom …

A Comparative Study Of Urban And Natural Nesting Gray Hawks In The Lower Rio Grande Valley Of South Texas, Evan Farese

Theses and Dissertations

Urbanization can negatively impact birds of prey, which occupy higher trophic levels. The Gray Hawk (Buteo plagiatus) is a neotropical raptor inhabiting the Lower Rio Grande Valley (LRGV) of Texas, where it is threatened. The species’ ecology has been little studied and it is unclear how urbanization may affect population viability. I studied Gray Hawk reproductive success, diet, and thermal ecology at nests in urban and natural habitats in the LRGV. Camera trap data showed adults at urban nests delivered mostly avian prey, while mammalian prey was most frequently brought to natural nests. Data from temperature loggers showed …

Knowledge And Perceptions About Feeding And Growth In Infants And Young Children Among Early Interventionists, Kayla Chambers

Electronic Theses and Dissertations

Nutrition plays a vital role in early childhood, where rapid growth and development occur. This study investigates Early Interventionists’ (EIs) nutrition knowledge and perceptions of nutrition-related concerns in children receiving services through the Tennessee Early Intervention System (TEIS). An electronic survey was distributed to EIs in Tennessee to assess their nutrition knowledge and perceptions and to identify nutrition concerns. Findings revealed that most EIs have never received any nutrition education, despite being asked for nutrition guidance by families and being open to nutrition-related training. The EIs reported seeing nutrition concerns such as growth delays, food refusal, and picky eating, but …

Therapeutic Applications Of A Novel Humanized Monoclonal Antibody Targeting Chemokine Receptor Ccr9 In Pancreatic Cancer, Hannah G. Mcdonald, Anna M. Reagan, Charles J. Bailey, Mei Gao, Muqiang Gao, Angelica L. Solomon, Michael J. Cavnar, Prakash Pandalai, Mautin Barry-Hundeyin, Megan Harper, Justin A. Rueckert, Ángela Turrero, Araceli Tobio, Anxo Vidal, Daniel Roca-Lema, Elia Álvarez-Coiradas, Pablo Garrido, Laureano Simón, Joseph Kim

Markey Cancer Center Faculty Publications

The relative failure of immune checkpoint inhibitors in pancreatic ductal adenocarcinoma (PDAC) despite having a dense, immunosuppressive tumor microenvironment highlights the need to target alternate/escape pathways. We have previously examined C–C chemokine receptor type 9 (CCR9) as a candidate immune checkpoint and developed a targeted, humanized monoclonal antibody (SRB2). Cytotoxicity of SRB2 was evaluated in vitro and in vivo. CCR9 expression on PDAC cells/tissues, immune components of patient-derived organoids (PDOs), and antibody-dependent cell-mediated cytotoxicity were examined. In PANC-1 and MIA PaCa-2 cell lines, we demonstrated highest CCR9 expression; however, no direct cytotoxic effect was observed with SRB2 treatment. In PANC-1 …

The Role Of Ucp2 In Pancreatic Cancer Metabolism And Its Potential As A Radiosensitizer, Emily Rieff

Dissertations and Theses (Open Access)

Uncoupling protein 2 (UCP2) is a mitochondrial protein that regulates the flow of protons down the gradient established by the electron transport chain (ETC) without generating ATP, thus uncoupling the proton gradient from ATP generation. In tumors, specifically pancreatic cancer (PDAC), the ETC is highly stimulated to generate the copious ATP needed by the tumor to grow. However, extended ETC use increases reactive oxygen species (ROS), and if left unchecked, results in cell death. To evade this, PDAC employs antioxidant strategies, including upregulating UCP2 in tumor cells. In addition to its uncoupling function, UCP2 indirectly controls ROS levels by maintaining …

Effects Of Snacking Habits On Overall Health And Cognition Of University Students, Kelly Shaw

Electronic Theses, Projects, and Dissertations

Title: Effects of Snacking Habits on Overall Health and Cognition of University Students

Authors: Shaw, K., Tolar-Peterson, T., & Malik, N.

Affiliation: College of Natural Sciences, Department of Health Science and Human Ecology, California State University, San Bernardino 5500 University Parkway, San Bernardino, CA 92407

Background and Aims: Snack consumption is increasing, and university students may be particularly susceptible to unhealthy snacking habits. Snacking patterns may affect students’ academic performance, mood, or body weight. This study assessed students’ snacking habits and its relationship with grade point average (GPA), mood, body mass index (BMI), and food insecurity.

Methods: Using an …

Assessing Phenotypic Variation Across Generations Of Iberodes Littoralis Under Climate Change, Mariangel M. Fernandez Silva

Electronic Theses, Projects, and Dissertations

The consequences of climate change and anthropogenic activity have decreased wildlife biodiversity. Plants are particularly vulnerable to climate change due to their large biomass on the planet and sessile nature. A particularly useful method for plants to cope with environmental changes is phenotypic plasticity. Adjusting morphological traits through plasticity can help plants cope with environmental changes. Furthermore, plastic responses can be passed down through methods such as changes in DNA sequence or epigenetic modifications.

Our research project focuses on an herb, Iberodes littoralis, a species of concern native to the Siberian Peninsula with approximately thirteen extant populations. The data that …

Estimation Of Nitrates In Southern California Water Resources, Sarath Babu, Sarath Babu

Electronic Theses, Projects, and Dissertations

The research examines the water quality of Southern California, focusing on nitrate (NO₃) levels. The study aims to provide insights into potential health disparities stemming from disproportionate exposure to these contaminants, particularly in underserved communities. Utilizing advanced analytical tools such as the UV spectrophotometer AquaMate Plus, water samples (n=40) were analyzed to assess nitrate levels compared to previous data (n=70 Approx.). The UV spectrophotometer operates based on measuring the absorbance of light intensity by the analyzed samples, providing valuable data for water quality assessment. Preliminary findings suggest varying levels of nitrate presence across the studied regions (0.4-43 ppm), …

Awareness And Attitudes Toward Diabetes Among Students In The California State University System, Cesar Fajardo

Electronic Theses, Projects, and Dissertations

Background and Aims: The prevalence of type 2 diabetes has been increasing globally. Studies examining patient awareness and attitudes toward diabetes have been conducted and found that diabetes education does increase patient knowledge but has no effect on attitudes. Additionally, many of these studies did not assess university students’ views on type 2 diabetes. Given that type 2 diabetes incidence is increasing among young adults, more attention must be given to this population. Therefore, the aim of this study was to investigate CSUSB students' understanding and attitudes toward type 2 diabetes.

Methods: A cross-sectional study using electronic surveys with …

The Reliability And Validity Of Accelerometry And Gnss, Christine Coniglio

Electronic Theses and Dissertations

This dissertation seeks to evaluate the reliability, validity, and utility of accelerometers and GNSS in sport-like scenarios, assess accelerometry-derived metrics and GNSS for distance prediction, and explore how accelerometer placement, specifically chest and back, affect the detection of various locomotor events. In Study I, the purpose was to investigate the accelerometer reliability and predictive capabilities of accelerometers and GNSS to predict a known distance. Thirty physically active female participants (mean height = 163.6 ± 6.8 cm, mean weight = 57.8 ± 8.7 kg) completed jogging trials over two courses (5m and 20m). Each participant wore two tri-axial accelerometers (100Hz) and …

The Forest Through The Trees: Modeling The Ecological Niche And Potential Assemblage Distribution Of Oak-Hickory Forests Across The Eastern U.S., Elijah Worley

Electronic Theses and Dissertations

Understanding how tree species’ ranges and populations may shift by the end of the 21^st Century is critical for effective conservation and forest management. Ecological niche models are widely used to predict species distributions by modeling the relationships between environmental variables and species presence. While numerous models have been developed for individual tree species across the USA, there is limited research on how tree species assemblages, such as oak-hickory forests, will respond to changes in climate over time. Maxent modeling software was used to predict potential shifts in suitable habitat for the oak-hickory forest type, based on environmental variables …

Unraveling The Unknown: Characterization Of A Novel Antimicrobial Compound Found In Klebsiella Pneumoniae, Jasmal Cruz Perez

Electronic Theses and Dissertations

Klebsiella pneumoniae, a pathogenic bacterium resistant to numerous antimicrobials, is a worldwide health issue due to its ability to cause healthcare-associated diseases such as pneumonia and urinary tract infections. This study investigated an antimicrobial compound secreted by K. pneumoniae. The compound was found to possess inhibitory activity against Enterobacteriaceae members, including other Klebsiella pneumoniae strains, Citrobacter freundii, and Enterobacter cloacae. The inhibitory activity is mediated via a secreted protein that is active across a wide range of pH and temperature conditions. Higher concentrations of the compound enhanced antimicrobial activity. The antimicrobial protein suppressed biofilm formation during both …

Exploring The Antagonistic Polymicrobial Interaction Of Alcaligenes Faecalis With Prokaryotic And Eukaryotic Microorganisms, Aly Hargrave

Electronic Theses and Dissertations

Alcaligenes faecalis, a generally nonpathogenic, Gram-negative bacterium, has been observed by our laboratory to possess antagonistic activity against antimicrobial-resistant genera Staphylococcus, Candida, and Bacillus. This study focused on exploring the inhibition mechanism of A. faecalis against the three genera, with an emphasis on S. aureus. A library of A. faecalis transposon mutants was constructed and identified fourteen possible genetic factors linked to this inhibitory activity. Characterization of this inhibition indicated that A. faecalis suppressed S. aureus planktonic and biofilm growth via a contact-dependent mechanism, with pronounced effects during biofilm attachment phase. In a Caenorhabditis elegans …

Impact Of Shear Flow On Marine Biofilms Using Hyperspectral Imaging, Shantanu Mahesh Kore

All Theses

Various microbial communities in marine biofilms cause biofouling on submerged surfaces, posing challenges to marine industries. Despite their ecological and economic importance, biofilms' biomechanical and biochemical responses to hydrodynamic shear stress are insufficiently understood, particularly in dynamic flow conditions. To fill this gap, our study uses an innovative fiber-optic hyperspectral imaging (HSI) system and a supercontinuum laser source to examine marine biofilms' structure, composition, and detachment behavior at different shear stress levels.

To detect spectral and spatial heterogeneity in live biofilms without labeling, we created a custom imaging pipeline that captures reflectance spectra in the 600-850 nm range, targeting microbial …

The Impact Of Irrigation On Agricultural Land Values In The United States Southeast And An Analysis Of Farmland Prices In South Carolina, Christopher L. Thomas

All Theses

Land is a key component of the agriculture industry in the United States. According to the United States Department of Agriculture, farm real estate (land and buildings) accounted for 82.8% of total US farm sector assets in 2022, consequently making land the largest source of wealth in a farmer’s investment portfolio and the primary source of collateral used for farm operating loans. The Southeastern region of the United States has seen rapid farmland appreciation and intense population growth in recent years. This research investigates the rising farmland prices across the Southeast from both a regional and state-level perspective. Chapter Two …

Berm-Leveling Machine For Peach Orchards: Incorporation Of Tree-Sensing Feature And Profitability Study, Shubham Singh

All Theses

Root collar excavation extends the productive life of peach trees in Armillaria root rot-infested soil, but the process requires trees to be planted on berms. Berm-planted peach orchards restrict the mobility of workers and machinery and can lead to increased erosion in furrows on either side of the berms. The current inter-tree berm-leveling machine depends on the driver's command to dodge the peach trees. The objectives of this thesis were to (1) integrate the feeler, infrared, and LiDAR-based tree sensing systems in the berm-leveling machine to make the soil excavating head's movement independent, (2) measure the impact of finger weeder-based …

Soil-Based Emissions And Context-Specific Climate Change Planning To Support The United Nations (Un) Sustainable Development Goal (Sdg) On Climate Action: A Case Study Of Georgia (Usa), Davis G. Nelson

All Theses

Soil-based emissions from land conversions are often overlooked in climate planning. The objectives of this study were to use quantitative data on soil-based greenhouse gas (GHG) emissions for the state of Georgia (GA) (USA) to examine context-specific (temporal, biophysical, economic, and social) climate planning and legal options to deal with these emissions. Currently, 30% of the land in GA has experienced anthropogenic land degradation (LD) primarily due to agriculture (64%). All seven soil orders were subject to various degrees of anthropogenic LD. Increases in overall LD between 2001 and 2021 indicate a lack of land degradation neutrality (LDN) in GA. …

Sacred Grounds: A Risk Assessment Framework Developed For Historic African American Cemeteries Threatened By Sea Level Rise In Georgetown, Sc, Jocelyn Patterson

All Theses

ABSTRACT

This study acknowledges historic African American cemeteries as vital cultural and historical landmarks that preserve the stories and traditions of often overlooked communities. However, coastal cemeteries, particularly in Georgetown, South Carolina, face increasing environmental threats. These burial sites risk severe damage or complete loss as climate change accelerates from sea level rise, flooding, storm surges, neglect, development pressure, and land development.

This research develops and considers a risk assessment framework to address the risks of cemeteries proximity to water, elevation, flood risk, and cultural significance. The research employs Geographic Information Systems (GIS) mapping, historical records, and site analysis to …

Biodegradable Active Packaging: Exploring The Antimicrobial Efficacy And Compostability Of Chitosan-Based Films, Rachel Mchugh

All Theses

The overarching purpose of this experiment is to create an active packaging component that has both antimicrobial and biodegradable properties specifically designed for poultry food products. For the scope of this study, the packaging component created would theoretically be used in conjunction with another material, such as a poultry tray which are commonly used for fresh poultry products. Based on the scope and purpose of this experiment, biopolymer films and coatings were considered after the initial literature review. Due to the goals of the packaging component, chitosan, a polysaccharide shown to have antimicrobial properties and an inherent ability to biodegrade, …

Anaerobic Soil Disinfestation As A Non-Chemical Management Option Against Fungi And Heterotrophic Bacteria In Organic Sweetpotato Production, Meredith Tucker

All Theses

North Carolina is the largest producer of sweetpotatoes in the United States, with South Carolina increasing farming acreage to 2,501 in 2022 compared to 1,300 in 2017. Growers producing conventional and organic sweetpotatoes are equally challenged with managing fungal pathogens due to fungicide resistance or fungicides being unavailable due to environmental regulations, leading many to shift to growing organic crops. Anaerobic soil disinfestation (ASD) is a non-chemical pre-plant management option that uses a carbon amendment, irrigation, and plasticulture to establish an anaerobic environment in soils, killing soil microbes. A greenhouse study was conducted to assess ASD on fungal pathogens and …

Identification And Environmental Impact Of 35 Different Cut Flower Retail Packages In The U.S.A., Mary Kathryn Gillespie

All Theses

Cut flowers represent 35-billion dollars of global spending with 10-million dollars per day in the U.S.A; each transaction involving packaging. Across the country, cut flower packaging is inconsistently sized, laminated, colored, and unlikely to be recycled. 35 unique cut flower packages were collected across 16 different marketplaces, representing 88% of top grocery retailers. Measuring the materiality of the predominately unlabeled and inconsistent packaging collected required calorimetry equipment, such as Fourier-transform Infrared Spectroscopy, Differential Scanning Calorimeter, and precision gauges. Results unveil five materials representing the cut flower collection: Oriented Polypropylene (OPP), High-Density Polyethylene (HDPE), Paper, Polyethylene Terephthalate (PET), and Nonwoven Polypropylene …

Behavior Change: Actions To Restore Piedmont Prairie And Adopt Sustainable Behavior, Kacey Marie Kaibery

All Theses

Restoration of an ecosystem or adoption of sustainable behavior could benefit the environment. Valuable ecosystem services, like water filtration and habitat for pollinators, inspire investigation into the restoration of grasslands, like Piedmont prairies. This research endeavors to determine the impact of disturbance type (tilling, herbicide, mowing, fire, control with seed or no seed). The second-year aboveground biomass was harvested, identified, counted, and weighed to understand plant species’ response after the application of the disturbance. Target species and plant community diversity did not significantly vary between disturbance types. Planting methods (hand dispersal, drill, one-month and six-month transplanting) on Asclepias tuberosa establishment …

Fully Bio-Based, Flexible, Non-Isocyanate Polyurethane Foams With High Lignin Content For Protective Packaging Applications, Kathryn N. Evancho

All Theses

Polyurethane foam is a valuable material with applications across the automotive, packaging, construction, appliance, and furniture industries. However, traditional polyurethane foams are made from petroleum-based precursors including harmful isocyanates. Isocyanates are the leading cause of workplace asthma and are made from toxic phosgene gas, representing a challenge to green chemistry principles such reducing risk and environmental footprint. To address these concerns, non-isocyanate polyurethane (NIPU) foams have been the focus of research for the past decade, but they still face significant challenges in achieving commercial viability. NIPUs replace the traditional polyurethane reaction with a safer, non-isocyanate process most often employing cyclic …

Effects Of Common Herbicides On Organismal Interactions, Robert Chamberlin

All Theses

Pesticides are applied to agricultural lands to prevent undesirable weeds, animal pests, and fungi from harming crops and reducing crop yields. Pesticides are used in croplands at increasing rates around the world and have become an essential component of farming in recent decades. Often, rain, wind, and soil permeation move pesticides from farmlands to natural environments. Such contamination of natural environments by pesticides may threaten species by altering their behaviors, bodily functions, and the ways in which individuals interact. Atrazine and glyphosate are among the most widely used herbicides around the world and are useful in eliminating weeds, primarily in …

Improved Management Strategies For Botrytis Fruit Rot, Macrophomina Root Rot, And Verticillium Wilt In California Strawberry Production, Joseph A. Ramirez

Master's Theses

Strawberry production in California is limited by plant diseases such as Macrophomina root rot (MRR) caused by Macrophomina phaseolina, Verticillium wilt (VW) caused by Verticillium dahliae, and Botrytis fruit rot (BFR) caused by Botrytis cinerea. Current disease management strategies are compromised due to fumigant regulations or ineffective disease management practices. This thesis investigated methods to potentially improve the management of these three diseases. Two host plant resistance evaluations for MRR and VW were conducted for the 2022-2023 and 2023-2024 growing seasons. Sixty-three strawberry genotypes were screened in both years. All plants were exposed to the pathogen via artificial …

The Development Of Magmas Inhibition As A Potential Therapeutic Target In Temozolomide Resistant Glioma, Jennifer Duyen Tran

Electronic Theses, Projects, and Dissertations

Glioblastoma (GBM) is a highly aggressive brain tumor that remains a significant challenge in neuro-oncology today. Approximately 90% of GBM patients experience tumor recurrence within one to two years of diagnosis. Therefore, due to its high resistance to conventional therapies, new treatment strategies are urgently needed. The current standard of care for GBM is the chemotherapeutic Temozolomide (TMZ). TMZ is an oral alkylating agent, it functions by delivering a methyl group to purine bases. The development of resistance to TMZ is partially regulated by the DNA repair enzyme O6- methylguanine-DNA methyltransferase (MGMT). Despite the potential shown by MGMT promoter methylation …

Engineering An In Vitro Hipsc-Derived Cardiac Tissue Construct For Regenerative Medicine Applications, Larry Mendoza

Electronic Theses, Projects, and Dissertations

Cardiovascular disease is the leading cause of mortality across the world with invasive surgeries and pharmaceutical intervention commonly associated with urgent care. Moreover, congenital, progressive, and hereditary heart diseases rely on many forms of medical prevention and intervention (pharmaceuticals, x-rays, bloodwork, etc.). Developing a three-dimensional (3D) cardiac tissue engineered in vitro model to study human cardiac physiology can revolutionize biomedical research. The purpose of this research is to optimize natural & synthetic biomaterials to fuse cardiac stem cells and synthesize a 3D-printed in vitro model for disease modeling, drug delivery, and regenerative medicine. Natural biopolymers needed for such innovations are …

Passive Electronic Cigarette Exposure Initiates Squamous Metaplasia In A Developing 3d Human Tracheobronchial Epithelial Tissue In-Vitro Model, Edgar Rolon

Electronic Theses, Projects, and Dissertations

The prevalence of vaping has rapidly increased. ECs are often perceived as less harmful than tobacco cigarettes. A recent meta-analysis showed electronic cigarette (EC) aerosols adversely affected the respiratory system, similar to cigarette smoke, with some effects specific to EC use. Failure to prevent these alterations could lead to life-long comorbidities, and predisposition to respiratory diseases, including COPD and squamous cell carcinoma. While these effects are the result of active vaping, EC aerosols can also settle on indoor surfaces, be absorbed passively through the skin or orally, enter the blood stream, and subsequently expose internal organs, including the airway basal …

Impact Of Water Quality On The Nutritional And Phytochemical Properties Of Culinary Herbs, Genesis Arroyo

Electronic Theses, Projects, and Dissertations

Water quality significantly affected food safety, especially since contaminated water impacted crop growth and the nutrient composition of foods. Nitrates occur naturally in vegetables. However, when introduced into the food supply through water contamination, excessive levels disrupted the natural physiological processes of vegetables and other agricultural plants. These excessive levels eventually led to nutrient imbalances, decreased phytochemical concentrations, and potential health risks such as the formation of carcinogenic N-nitroso compounds in humans. This study evaluated the effects of nitrates on the nutritional outcomes of food crops, focusing on commonly used culinary herbs. While synthesizing findings from relevant peer-reviewed articles, I …

Effects Of Environmentally Relevant Concentrations Of Roundup On Oxidative-Nitrative Stress, Cellular Apoptosis, Prooxidant-Antioxidant Homeostasis, Renin And Cyp1a Expressions In Goldfish: Molecular Mechanisms Underlying Kidney Damage During Roundup Exposure, Md Imran Noor, Md Saydur Rahman

School of Earth, Environmental, & Marine Sciences Faculty Publications

Roundup is one of the most widely used glyphosate-based harmful herbicides in the United States as well as globally, which poses a severe risk for terrestrial and aquatic organisms. In order to identify the detrimental effects of Roundup exposure in aquatic organisms, we investigated the environmentally relevant concentrations of Roundup exposure (low dose: 0.5 μg/L and high dose: 5.0 μg/L for 2 weeks) on renin expression, oxidative-nitrative stress biomarkers (e.g., 2,4-dinitrophenol, DNP; and 3-nitrotyrosine protein, NTP), prooxidant-antioxidant enzymes expressions (e.g., superoxide dismutase, SOD; and catalase, CAT), cellular apoptosis, and cytochrome P450 1A (CYP1A) mRNA levels in the kidneys of goldfish …