Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Discipline
Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 1261 - 1290 of 254184

Full-Text Articles in Life Sciences

Detection Of Early Browning Inside Fragrant Pears Based On Time-Frequency Images Of Acoustic And Vibration Signals, Zhang Feng, Yu Junyang, Xiao Ping, Zhang Hui, Wang Kai Jun 2026

Detection Of Early Browning Inside Fragrant Pears Based On Time-Frequency Images Of Acoustic And Vibration Signals, Zhang Feng, Yu Junyang, Xiao Ping, Zhang Hui, Wang Kai

Food and Machinery

[Objective] To explore the detection efficacy of models constructed by combining time-frequency image features of the fragrant pears in acoustic and vibration with machine learning algorithms.[Methods] Acoustic and vibration signals are collected from fragrant pears exhibiting varying degrees of internal browning using a custom-built non-destructive detection device. Time-frequency images are transformed using the short-time Fourier transform (STFT),continuous wavelet transform (CWT), and the S-transform. Then, texture feature parameters in 15 time-frequency domains are extracted, respectively, from 3 types of time-frequency images using the Gray-Gradient Co-occurrence Matrix (GGCM). Additionally, correlation analysis is performed between these feature parameters and the fragrant pear browning …


A Rapid Quality Inspection Method For Refrigerated Fish Based On Improved Plsr-Yolov 13, Wu Xia, Tian Erlin, Wang Qifeng, Zhu Cong Jun 2026

A Rapid Quality Inspection Method For Refrigerated Fish Based On Improved Plsr-Yolov 13, Wu Xia, Tian Erlin, Wang Qifeng, Zhu Cong

Food and Machinery

[Objective] To effectively solve the problems of complex operation, long time consumption, insufficient accuracy, and difficulty in balancing speed and accuracy in existing quality inspection methods for refrigerated fish.[Methods] On the basis of a food quality inspection system that integrates multiple detection technologies, a rapid quality inspection method for refrigerated fish that combines hyperspectral data and machine vision is constructed. The hyperspectral imaging technology is employed to collect spectral data of refrigerated fish, and the data are preprocessed and input into an improved partial least squares regression model to achieve accurate determination of total volatile basic nitrogen content, and then …


Effects Of Synergistic Preservation Technology On Quality And Shelf Life Of Meat Buns Under Room Temperature Storage, Li Shumin, Yu Xin, Du Ting, Wu Yina, Du Xinjun Jun 2026

Effects Of Synergistic Preservation Technology On Quality And Shelf Life Of Meat Buns Under Room Temperature Storage, Li Shumin, Yu Xin, Du Ting, Wu Yina, Du Xinjun

Food and Machinery

[Objective] To develop a synergistic preservation scheme for meat buns that balances safety and quality stability, thereby achieving a 7-day safe shelf life during room temperature storage.[Methods] High-temperature high-pressure sterilization or stewing is adopted as the core pretreatment procedure. The wrapper is added with calcium propionate (0.15 g/kg ),while the meat filling is incorporated with compound preservatives including sodium diacetate (1.5 g/kg ), potassium sorbate (0.060 g/kg ), and ε-polylysine (0.20 g/kg ). Combined with the synergistic effects of modified atmosphere packaging and preservation pads, a storage environment is constructed to continuously inhibit microbial regrowth and delay oxidation.[Results] The meat …


Effects Of Air-Impingement Jet Drying On Drying Characteristics , Color , Nutritional Quality And Antioxidant Capacity Of Prunus Armeniaca L. Cv. Diaogan, Cheng Hui, Gao Jianrui, Li Chunqi, Lu Chunxia Jun 2026

Effects Of Air-Impingement Jet Drying On Drying Characteristics , Color , Nutritional Quality And Antioxidant Capacity Of Prunus Armeniaca L. Cv. Diaogan, Cheng Hui, Gao Jianrui, Li Chunqi, Lu Chunxia

Food and Machinery

[Objective] Air-impingement jet drying technology is applied to explore the influence of drying temperature and air velocity on the drying characteristics, color, nutritional quality and antioxidant capacity of Prunus armeniaca L.cv.Diaogan.[Methods] P.armeniaca samples are blanched and then dried at different temperatures (40,45,50,55,60 ℃) and air velocity (6,8,10,12,14 m/s), respectively. The drying characteristics, color, soluble sugar contents, total acid contents, total phenol contents and antioxidant activity were measured.[Results] The air-impingement jet drying of P.armeniaca primarily falls into a rate-falling drying process. The drying curve and drying rate curve are significantly (P<0.05) affected by drying temperature but not significantly (P>0.05) by air velocity. The contents of soluble sugar, ascorbic …


Effects Of Gardenia Extract On Body Weight And Intestinal Mucosal Microbiota In Obese Mice, Liu Qin, Deng Na, Zhou Mengsi, Tan Zhoujin Jun 2026

Effects Of Gardenia Extract On Body Weight And Intestinal Mucosal Microbiota In Obese Mice, Liu Qin, Deng Na, Zhou Mengsi, Tan Zhoujin

Food and Machinery

[Objective] To explore the effects of gardenia extract on intestinal mucosal microbiota and body weight in mice fed a high -fat diet, and to explore the microecological mechanism of gardenia extract in obese mice.[Methods] The potential mechanism of gardenia extract in treating obesity is predicted by network pharmacology and molecular docking technology. Additionally, 24 SPF C 57BL/6 male mice are randomly assigned into a blank group (8 mice ) and a model group (16 mice ). The model group receives a high-fat diet for 12 weeks, while the blank group receives an ordinary diet. After modeling, 12 successfully modeled mice …


Anti-Inflammatory Effect Of Sipunculus Nudus Collagen Peptide And Its Mechanism In Skin Wound Repair Promotion In Mice, Huang Haoyang, Zhang Junyi, Shen Weiqiang, Cao Wenhong, Lin Haisheng Jun 2026

Anti-Inflammatory Effect Of Sipunculus Nudus Collagen Peptide And Its Mechanism In Skin Wound Repair Promotion In Mice, Huang Haoyang, Zhang Junyi, Shen Weiqiang, Cao Wenhong, Lin Haisheng

Food and Machinery

[Objective] To explore the repairing effect of Sipunculus nudus collagen peptide (SNCP ) on skin wounds and clarify its potential efficacy in anti -inflammation, oxidative stress regulation,and wound repair promotion.[Methods] Collagen is extracted from the body wall of S.nudus via the hot water extraction method, and SNCP is subsequently prepared through enzymolysis and ultrafiltration. Then,the lipopolysaccharide (LPS)-induced RAW 264.7 cell inflammation model and the whole cortex wound inflammation model in mice are employed to detect the effect of SNCP on cytotoxicity and cell morphology, as well as the inflammatory, antioxidant, and growth factors in mouse wound tissues.[Results] At 50 to …


Flavonoid Extraction And Antioxidant Activity Of Daidai's Physiological Fallen Fruits, Zhang Xinyi, Li Qili, Li Zhijian, Ge Hanxiao, Shao Yuting, Xiao Yecheng, Li Tao, Su Donglin Jun 2026

Flavonoid Extraction And Antioxidant Activity Of Daidai's Physiological Fallen Fruits, Zhang Xinyi, Li Qili, Li Zhijian, Ge Hanxiao, Shao Yuting, Xiao Yecheng, Li Tao, Su Donglin

Food and Machinery

[Objective] To explore a green extraction process for flavonoids from Daidai's physiological fallen fruits and to analyze the composition, microstructure, and antioxidant activity of the extracts.[Methods] Flavonoids were extracted from Daidai's physiological fallen fruits using an ethanol-water solution combined with ultrasonic-assisted extraction. The extraction conditions were optimized through single-factor experiments. The flavonoid composition of the extract was determined by high-performance liquid chromatography (HPLC ), its microstructure was observed using scanning electron microscopy (SEM ), and in vitro antioxidant activity was evaluated by DPPH radical 苏东林(1979—),男,湖南省农业科学院研究员,博士。E-mail:sdl791228 @163.com scavenging, ABTS radical scavenging, and FRAP assays.[Results] The optimal extraction conditions were: 75% ethanol, …


Effect Of Garlic Paste Addition On Volatile Flavor Substances In Crayfish Tails, Tu Jianqiu, Fu Wenjun, Yang Xiaocong, Jing Lixiang, Zhu Jing, Li Jianfang Jun 2026

Effect Of Garlic Paste Addition On Volatile Flavor Substances In Crayfish Tails, Tu Jianqiu, Fu Wenjun, Yang Xiaocong, Jing Lixiang, Zhu Jing, Li Jianfang

Food and Machinery

[Objective] To investigate the effect of garlic paste addition on volatile flavor substances and color attributes of crayfish tails, and provide a theoretical basis for flavor standardization in industrial production.[Methods] This paper adopts gas chromatography-ion mobility spectrometry (GC-IMS ) combined with colorimetric analysis to study the characteristics of volatile components and color parameters (L*,a*,b* values ) of crayfish tails under garlic paste additions of 0%,4.4%,8.8%,13.2%,17.6%,and 22.0%. Principal component analysis (PCA ) is applied to analyze flavor differences.[Results] Colorimetric analysis reveals that garlic paste addition significantly decreases L* from 45.69 to 34.50 and b* from 39.24 to 29.94 (P<0.05), while a* values show a significant difference under the garlic paste addition of more than 13.2% (P<0.05). A total of 53 volatile substances are identified by GC-IMS, and their total content increases gradually with the rising garlic paste addition (highest value in the 22% group at 18 749 and lowest value in the control group at 10 758). Camphene, 2-ethylbutanoic acid, 2-methylbutyl acetate, allyl isothiocyanate, and heptanal are identified as intrinsic flavor markers of crayfish meat. Key contributors to flavor include camphene, 1-heptanol, 3-pentenenitrile, 2,3-pentanedione, diacetyl, and isopropanol. PCA shows that the 13.2%~17.6% group exhibits significantly different flavor characteristics from other groups (PC1 contribution rate of 76%).[Conclusion] Under the garlic paste addition of 8.8%~13.2%, the optimal balance between flavor and color attributes is achieved, while excessive addition of more than 17.6% results in aldehyde overaccumulation and accelerated browning.


Optimization Of Polysaccharide Extraction Process From Chlorella Vulgaris And Their Antioxidant Activity, Wang Hua, Zhang Pengjuan, Xue Xiaodong, Liu Ziyu Jun 2026

Optimization Of Polysaccharide Extraction Process From Chlorella Vulgaris And Their Antioxidant Activity, Wang Hua, Zhang Pengjuan, Xue Xiaodong, Liu Ziyu

Food and Machinery

[Objective] To optimize the extraction process of Chlorella vulgaris polysaccharides using response surface methodology and analyze their antioxidant activity.[Methods] The extraction rate of C.vulgaris polysaccharides is used as an evaluation index, and the single factor method is used to select the three factors with the greatest impact on the polysaccharide extraction rate. Box-Behnken design is used to optimize the extraction process. The antioxidant capacity of C.vulgaris polysaccharides is evaluated by DPPH scavenging rate, hydroxyl radical scavenging rate and total reducing power.[Results] The optimal extraction conditions are determined as follows: sodium hydroxide mass concentration of 1.70 g/100 mL, extraction temperature of …


Advances In Extraction And Detection Technologies For Veterinary Drug Residues In Food, Luo Qiaoqiao, Yuan Yufeng, Jia Yunzhu, Chen Qingya, Tan Fang, Zhao Xin Jun 2026

Advances In Extraction And Detection Technologies For Veterinary Drug Residues In Food, Luo Qiaoqiao, Yuan Yufeng, Jia Yunzhu, Chen Qingya, Tan Fang, Zhao Xin

Food and Machinery

Veterinary drug residues constitute a global challenge that poses significant threats to food safety and public health. This review systematically summarizes recent advances in detection technologies for veterinary drug residues in food, with a particular focus on the performance characteristics and application status of sample extraction techniques (including liquid-liquid extraction, solid phase extraction, accelerated solvent extraction, QuEChERS, and magnetic solid phase microextraction ),as well as analytical methods (including chromatographic-mass spectrometric techniques, immunoassays, capillary electrophoresis, biosensors, and quantum dots -based approaches ). Comparative analysis reveals that accelerated solvent extraction (ASE ) and QuEChERS have emerged as mainstream pretreatment techniques owing to …


Research Progress On The Extraction Of Functional Components From Chinese Baijiu Distiller's Grains And Their High-Value Applications, Wang Yanyong, Guo Shuanghai Jun 2026

Research Progress On The Extraction Of Functional Components From Chinese Baijiu Distiller's Grains And Their High-Value Applications, Wang Yanyong, Guo Shuanghai

Food and Machinery

Chinese Baijiu distiller's grains represent a major by-product of the Baijiu brewing industry. The solid-state or liquid-state multi-strain fermentation process endows them with complex chemical compositions and functional properties, conferring considerable potential for further development. This study describes the chemical composition of Chinese Baijiu distiller's grains, discusses the differences in functional components arising from the brewing processes of various Baijiu aroma types, and evaluates advanced extraction techniques targeting core components, including proteins, dietary fibers, and polyphenols. The biological activities of Chinese Baijiu distiller's grain extracts, such as antioxidant, antihypertensive, and immunoregulatory effects, as well as their underlying mechanisms of action, …


Preparation And Fermentation Characteristics Of Fuqu Fortified By Yeast Community Based On Jiangxiang Daqu, Wei Chunhui, Xu Ziheng, Ying Chao, Zhao Huan, Ren Zhiqiang Jun 2026

Preparation And Fermentation Characteristics Of Fuqu Fortified By Yeast Community Based On Jiangxiang Daqu, Wei Chunhui, Xu Ziheng, Ying Chao, Zhao Huan, Ren Zhiqiang

Food and Machinery

[Objective] To solve the problems of single strain, limited fermentation capability, and weak environmental tolerance of yeast Fuqu.[Methods] The yeast community of Jiangxiang Daqu is utilized as the strain source, and bran is used as the raw material to prepare a multi-yeast mixed Fuqu with fortified function. The preparation conditions of yeast Fuqu are optimized by single factor tests combined with orthogonal tests. Then, the acid, ethanol, and high-temperature resistance are systematically evaluated, while the microbial community structure is analyzed via internal transcribed spacer (ITS) rDNA high-throughput sequencing.[Results] The optimal process parameters are a moisture content of 45%,an incubation time …


Realistic Dilemmas And Optimization Pathways Of Investigative Collaboration In Crimes Of Producing And Selling Toxic And Harmful Food From The Perspective Of Collaborative Governance, Yu Baohua Jun 2026

Realistic Dilemmas And Optimization Pathways Of Investigative Collaboration In Crimes Of Producing And Selling Toxic And Harmful Food From The Perspective Of Collaborative Governance, Yu Baohua

Food and Machinery

Article 144 of the Criminal Law of the People's Republic of China explicitly stipulates the crime of producing and selling toxic and harmful food. An analysis employing analytical frameworks such as collaborative governance theory, intergovernmental relations theory, and digital governance theory reveals that an institutional framework for investigative collaboration concerning such crimes has preliminarily taken shape, and collaborative models at the practical operational level are undergoing continuous innovation. However, examined from the perspective of effectiveness, there remains considerable room for improvement in terms of information sharing, evidence conversion, and the execution mechanism of cross-regional and cross-departmental collaboration. The underlying causes …


Effects Of Wrinkled Pea Meal Replacement Powder On Glucose And Lipid Metabolism Disorders And Gut Microbiota In Mice, Cui Yajun, Hong Lingyu, Zhou Sumei, He Yuhua, Yao Yang, Hou Dianzhi Jun 2026

Effects Of Wrinkled Pea Meal Replacement Powder On Glucose And Lipid Metabolism Disorders And Gut Microbiota In Mice, Cui Yajun, Hong Lingyu, Zhou Sumei, He Yuhua, Yao Yang, Hou Dianzhi

Food and Machinery

[Objective] To investigate the effects of wrinkled pea meal replacement powder on glucose and lipid metabolism disorders and gut microbiota.[Methods] A 12-week intervention experiment was conducted using a high-fat diet-fed mouse model. Changes in key indicators,including mouse phenotypes,blood biochemical parameters, histopathology, and gut microbial composition, were systematically monitored.[Results] Wrinkled pea meal replacement powder significantly reduced fasting blood glucose, area under the blood glucose curve, and serum levels of total cholesterol, triglycerides, and low-density lipoprotein cholesterol in high-fat-fed mice (P<0.05). It effectively alleviated hepatic steatosis, reduced serum alanine aminotransferase levels (P<0.05), and significantly mitigated hepatocyte damage. Additionally, consumption of wrinkled pea meal replacement powder significantly regulated gut microecological imbalance induced by a high-fat diet, increased gut microbial richness, decreased the Firmicutes/Bacteroidetes ratio, and increased the relative abundance of beneficial bacteria, including norank_f_ Muribaculaceae, norank_o_ Clostridia _UCG- 014, and Bifidobacterium.[Conclusion] Wrinkled pea meal replacement powder has potential application value in improving glucose and lipid metabolism and regulating gut health.


Zinc Biofortification Technologies , Edible Safety , And Physiological Function Regulation In Edible Mushrooms, Wu Bingbing, Shi Nengpai, Su Jianwei, Hou Xuemei, Lin Xiangyang Jun 2026

Zinc Biofortification Technologies , Edible Safety , And Physiological Function Regulation In Edible Mushrooms, Wu Bingbing, Shi Nengpai, Su Jianwei, Hou Xuemei, Lin Xiangyang

Food and Machinery

Zinc (Zn) is an essential trace element for humans, and dietary zinc deficiency is an important global public health issue. Edible mushrooms have become highly promising carriers for zinc biofortification due to their natural mineral accumulation ability and the capacity to convert inorganic zinc into organically bound states with higher bioavailability. The enrichment and absorption of zinc, intracellular homeostasis regulation, and subcellular distribution patterns during the cultivation process of edible mushrooms were summarized, and the key factors influencing the efficiency of zinc biofortification, including fungal species, zinc sources (inorganic, organic, or nano forms ),application methods,and cultivation conditions, were evaluated. In …


Processing Mechanism And Efficacy Maintenance Strategy Of Ginseng Active Ingredients Based On Spectroscopy Technology, Yang Hantong, Kong Linghui, Jiang Mengmeng, Wang Yingxiu, Li Xiangguo Jun 2026

Processing Mechanism And Efficacy Maintenance Strategy Of Ginseng Active Ingredients Based On Spectroscopy Technology, Yang Hantong, Kong Linghui, Jiang Mengmeng, Wang Yingxiu, Li Xiangguo

Food and Machinery

Ginseng, as a medicinal and edible plant, has ginsenosides as its core active components, which are widely applied due to their physiological functions such as antioxidant and anti-inflammatory activities; however, their structures and activities are easily affected by food processing. During processing steps such as cleaning, steaming/boiling, and fermentation, temperature, pH, and microbial enzyme systems alter the types, contents, and stability of active components through reactions such as hydrolysis and oxidation. The intrinsic linkage mechanism of "component transformation-activity-bioavailability" constitutes a core focus and challenge in current research. This study, based on spectroscopic techniques including high-performance liquid chromatography (HPLC), nuclear magnetic …


Intelligent Temperature And Humidity Control System For Daqu Fermentation Rooms Using Smith Predictor-Based Fuzzy Pid, Yang Haili, Liao Xilong, Liu Sai, Hu Xinjun, Li Lan, Xie Liangliang, Chen Shan, Fei Lei Jun 2026

Intelligent Temperature And Humidity Control System For Daqu Fermentation Rooms Using Smith Predictor-Based Fuzzy Pid, Yang Haili, Liao Xilong, Liu Sai, Hu Xinjun, Li Lan, Xie Liangliang, Chen Shan, Fei Lei

Food and Machinery

[Objective] To address the strong delay and nonlinearity in the Daqu fermentation process.[Methods] This study proposes a coordinated control strategy combining a Smith predictor and fuzzy proportional -integral -derivative (PID). By establishing a mathematical model of the fermentation process, the Smith predictor is used to compensate for the delay. Combined with fuzzy rules, the PID parameters are tuned online, achieving precise control of temperature and humidity.[Results] Compared with traditional and fuzzy PID control, the temperature overshoot is reduced by 23.3% and 4.7%,respectively,while the adjustment time is shortened by 15.81% and 11.53%,respectively. For humidity, the overshoot is reduced by 31.4% and …


Effects Of Ozone Treatment At Different Concentrations On Storage Quality Of Fresh Peanuts, Yang Hui, Han Junhao, Xie Yongkang, Li Ping, Cao Shina Jun 2026

Effects Of Ozone Treatment At Different Concentrations On Storage Quality Of Fresh Peanuts, Yang Hui, Han Junhao, Xie Yongkang, Li Ping, Cao Shina

Food and Machinery

[Objective] To study the preservation effect of ozone on fresh peanuts.[Methods] "Four Red" fresh peanuts were used as experimental materials. Peanuts were treated with ozone at different concentrations (10,30,and 50 mg/L ) and stored at (4 ± 1) ℃ for 15 days. Edible quality, nutritional quality, and safety quality were measured every 3 days.[Results] The effects of ozone treatment on the post-harvest storage quality of fresh peanuts varied with concentration. Compared with the control group, medium-to-low concentration ozone treatment (≤30 mg/L ) effectively reduced peanut mass loss, inhibited mold development and microbial growth, maintained stable kernel color and good texture, …


Effects Of Thermal Processing Methods On Flavor Quality Of Fish Meat And Differential Formation Mechanisms : A Review, Zou Wanghua, Hou Xiaoyi, Liu Miao, Deng Na, Ni Panxianzhi, Li Hui, Fu Haohua, Wang Jianhui Jun 2026

Effects Of Thermal Processing Methods On Flavor Quality Of Fish Meat And Differential Formation Mechanisms : A Review, Zou Wanghua, Hou Xiaoyi, Liu Miao, Deng Na, Ni Panxianzhi, Li Hui, Fu Haohua, Wang Jianhui

Food and Machinery

Thermal processing is the most commonly used technique for fish products. Different thermal processing techniques vary in the degree of lipid oxidation, protein degradation, and Maillard reaction, as well as the synergy among these reactions, thereby differentially affecting flavor. Focusing on four typical thermal processing methods, steaming, boiling, frying, and roasting, this paper reviews the flavor change patterns in various types of fish products under different thermal processing conditions. Moreover, from the perspective of the synergy among lipid oxidation and decomposition, protein degradation, and the Maillard reaction, this paper systematically elucidates the formation mechanisms underlying the flavor substance enrichment and …


The Effect Of Discovery Learning Model On Students’ Critical Thinking Skills In Biology Learning At Sman 1 Dampelas, Umiyatul Rahma, Mursito S. Bialangi, Amalia Buntu, Fatma Dhafir, Raya Agni, Gamar B. N Shamdas Jun 2026

The Effect Of Discovery Learning Model On Students’ Critical Thinking Skills In Biology Learning At Sman 1 Dampelas, Umiyatul Rahma, Mursito S. Bialangi, Amalia Buntu, Fatma Dhafir, Raya Agni, Gamar B. N Shamdas

Jurnal Pendidikan Sains

Critical thinking skills are essential in biology learning; however, many classroom practices remain teacher-centered, limiting students’ opportunities to engage in higher-order thinking processes. Although previous studies have reported the potential of Discovery Learning to enhance critical thinking, evidence regarding its effectiveness in secondary biology education remains limited, particularly in the context of Indonesian high schools. Therefore, this study aimed to examine the effect of the Discovery Learning model on students’ critical thinking skills in biology learning at SMAN 1 Dampelas. A quasi-experimental study employing a nonequivalent control group design was conducted with 48 Grade XI science students, consisting of 24 …


Student Worksheets Based On The Local Wisdom Of Besilek Serawai For Sixth-Grade Elementary Science Learning, Mice Agustin, Tomi Hidayat, Irwandi Irwandi Jun 2026

Student Worksheets Based On The Local Wisdom Of Besilek Serawai For Sixth-Grade Elementary Science Learning, Mice Agustin, Tomi Hidayat, Irwandi Irwandi

Jurnal Pendidikan Sains

This study aims to develop a Student Worksheet (Lembar Kerja Peserta Didik [LKPD]) based on the local wisdom of Besilek Serawai for sixth-grade elementary science education and to evaluate its validity. The study employed the ADDIE development model, consisting of five stages: Analyze, Design, Develop, Implement, and Evaluate. The developed LKPD integrates Besilek Serawai, a traditional martial art of the Seluma community, with the concept of the human locomotor system in science instruction. The worksheet was validated by two subject-matter experts and two media experts. Material validation yielded an average score of 4.26, equivalent to a validity percentage of 85.2%, …


Elucidating The Mechanisms Of Complex Flavoprotein Dehydrogenases, Tyler Alt Jun 2026

Elucidating The Mechanisms Of Complex Flavoprotein Dehydrogenases, Tyler Alt

Dissertations

Flavins are ubiquitous redox active cofactors that can catalyze electron transfer between substrates. They offer the advantage of being able to stabilize a range of oxidation and protonation states, as well being amenable to covalent modification, which can further alter their activity. As such, the scope of reactions catalyzed by flavoproteins is broad and flavin has implications in a range of biological processes. The two predominant forms of flavin that serve as redox active cofactors are flavin adenine mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are functionally homologous. A major complication of studying flavoprotein dehydrogenases, and one that has …


Preoperative Low-Intensity Blood Flow Restriction Training Does Not Improve Quadriceps Strength Or Hypertrophy After Anterior Cruciate Ligament Reconstruction, Daniel Mcgurren, Kaycee Glattke, Molly Klanderman, Jedediah Lee, Anikar Chhabra, Arthur De Luigi Jun 2026

Preoperative Low-Intensity Blood Flow Restriction Training Does Not Improve Quadriceps Strength Or Hypertrophy After Anterior Cruciate Ligament Reconstruction, Daniel Mcgurren, Kaycee Glattke, Molly Klanderman, Jedediah Lee, Anikar Chhabra, Arthur De Luigi

Journal for Sports Neuroscience

Objectives: To investigate the efficacy of a preoperative low-intensity blood flow restriction (LI-BFR) training intervention for patients undergoing anterior cruciate ligament reconstruction (ACLR).

Design: Pilot study

Methods: Twenty-eight patients undergoing primary ACLR participated in either a LI-BFR training or sham intervention at a rate of 2 sessions per week for 2 weeks before surgery. Quadriceps strength and cross-sectional area were measured via handheld dynamometry and leg press test before and after the intervention before surgery and at 5 different time points after surgery, through 24 weeks after surgery.

Results: Adjusting for sex, time, and strength/hypertrophy at baseline, there were no …


Influence Of Smoking On Mlh1 Gene Polymorphism: Insights Into Colorectal Cancer Incidence, Ali Mohammed Al-Abedi, Rand Muhammed Abdul-Hussein Al-Husseini Jun 2026

Influence Of Smoking On Mlh1 Gene Polymorphism: Insights Into Colorectal Cancer Incidence, Ali Mohammed Al-Abedi, Rand Muhammed Abdul-Hussein Al-Husseini

Al-Bahir

Background: Colorectal cancer (CRC) is a leading cause of cancer-related mortality worldwide. The MutL Homolog 1 (MLH1) gene plays a critical role in DNA mismatch repair (MMR). Evidence suggests cigarette smoking increases CRC risk, potentially interacting with genetic factors.

Materials and Methods: This case-control study involved 50 Iraqi CRC patients and 50 age- and gender-matched healthy controls. The PCR-RFLP technique was employed for genotyping the -93G/A (rs1800734) polymorphism. Statistical analysis included Chi-square, Fisher's Exact Test, and logistic regression adjusted for confounders. Hardy-Weinberg Equilibrium (HWE) was assessed in controls.

Results: The frequency of the rs1800734 variant was significantly higher in CRC …


Dual-Action Antibacterial Agents Targeting Biofilm Reduction And Membrane Disruption In Staphylococcus Aureus, Rahmat Folashade Zakariyah, Waliyullahi Akorede Husain, Elohor Precious Samuel, Wonder Olanipekun Oloruntoba, Fatmah Opeyemi Saka, Khalid Ayinde Oyelami, Mariam Temitope Baker Jun 2026

Dual-Action Antibacterial Agents Targeting Biofilm Reduction And Membrane Disruption In Staphylococcus Aureus, Rahmat Folashade Zakariyah, Waliyullahi Akorede Husain, Elohor Precious Samuel, Wonder Olanipekun Oloruntoba, Fatmah Opeyemi Saka, Khalid Ayinde Oyelami, Mariam Temitope Baker

Al-Bahir

Antimicrobial resistance in Staphylococcus aureus is a serious global health problem as the organism rapidly develops resistance against widely used antibiotics. This study sought to investigate the antibacterial and antibiofilm properties of γ-Tocopherol, a natural compound from Ocimum gratissimum, as well as its synergism with 5-hydroxymethylfurfural (5-HMF) against S. aureus. The antibacterial activities and minimum inhibitory concentrations of the compounds were determined using agar diffusion and microbroth dilution methods, respectively. Biofilm reduction was evaluated using 2,3,5-triphenyltetrazolium chloride (TTC) assay and membrane integrity was evaluated using the crystal violet microtiter plate assay and the ion leakage assay. The results …


Evaluating The Efficacy Of Green-Method-Prepared Zinc Oxide Nanoparticles On Gene Expression Of Efflux Pumps In Klebsiella Pneumoniae Bacteria Isolated From Women With Gestational Diabetes, Wissam Qadry Mutaab, Rana J. Shaker Jun 2026

Evaluating The Efficacy Of Green-Method-Prepared Zinc Oxide Nanoparticles On Gene Expression Of Efflux Pumps In Klebsiella Pneumoniae Bacteria Isolated From Women With Gestational Diabetes, Wissam Qadry Mutaab, Rana J. Shaker

Al-Bahir

Vaginitis is one of the most common women's health problems, and its severity increases in pregnant women with gestational diabetes due to higher susceptibility to infection and increased rates of antibiotic resistance. The research problem: The increasing prevalence of multidrug-resistant isolates of Klebsiella pneumoniae and its association with AcrAB-TolC ejaculation pumps limits treatment efficacy, while the effect of green-method nanoparticles on gene expression of these pumps remains unclear. This paper aimed to detect the acrA and acrB genes in K. pneumoniae isolates and evaluate the effect of zinc oxide nanoparticles prepared with garlic and rosemary extracts on their gene expression. …


A Rho5-Cnc1-Dnm1 Signalling Module Coordinates Multi-Organelle Execution Of Regulated Cell Death In Yeast, Justin Bauer Jun 2026

A Rho5-Cnc1-Dnm1 Signalling Module Coordinates Multi-Organelle Execution Of Regulated Cell Death In Yeast, Justin Bauer

Theses and Dissertations

Regulated cell death is a conserved process with major implications for health and disease, yet how yeast cells integrate stress signals to determine survival versus death remains unclear. This thesis investigates Cyclin C (Cnc1), a conserved Mediator kinase module component with both transcriptional and mitochondrial roles. Together with Cdk8 Cnc1 primarily represses stress response genes, while upon stress it translocates to the mitochondria to induce mitochondrial fragmentation with the dynamin-related GTPase Dnm1. Using structure-guided separation-of-function mutants, I demonstrate that Cnc1-dependent death requires its mitochondrial function rather than its transcriptional role, and that its effector Dnm1 acts as a signaling node …


The Helminthic Status Of Selected Tanzanian Subterranean Rodents, The Root-Rats (Spalacidae: Tachyoryctes Splendens) And Emini Silvery Mole-Rats (Bathyergidae: Heliophobius Argenteocinereus Emini), Jestina V. Katandukila Jun 2026

The Helminthic Status Of Selected Tanzanian Subterranean Rodents, The Root-Rats (Spalacidae: Tachyoryctes Splendens) And Emini Silvery Mole-Rats (Bathyergidae: Heliophobius Argenteocinereus Emini), Jestina V. Katandukila

Tanzania Journal of Science

Root-rats (Tachyoryctes splendens) and emini silvery mole-rats (Heliophobius argenteocinereus emini) are common subterranean rodents that are essential component of the ecosystem. They serve as prey for small to medium-sized carnivores and birds of prey but their role as hosts to parasites is limited. The present study, explored the Gastrointestinal Tract (GIT) contents of 40 rodents (20 T. splendens and 20 H. a. emini, respectively) to acquire information on parasites diversity of these mammals. The GIT contents from subterranean rodents in a sex ratio of 1:1 were examined under a compound microscope to isolate helminths. The isolated helminths belonged to the …


Quercus Rubra (L.) Fine Root Trait Trade-Offs, Respiration, And Biomass Across A Midwestern U.S. Latitudinal Gradient, Emma L. Shedd, Andrew Burton, Carsten Kulheim, Molly A. Cavaleri Jun 2026

Quercus Rubra (L.) Fine Root Trait Trade-Offs, Respiration, And Biomass Across A Midwestern U.S. Latitudinal Gradient, Emma L. Shedd, Andrew Burton, Carsten Kulheim, Molly A. Cavaleri

Michigan Tech Publications

Aims: Regional climate changes may lead to shifts in root traits, affecting forest productivity and carbon release. Fine roots, which can respire significant proportions of fixed carbon dioxide back to the atmosphere, may adjust through changes in their respiration rates, morphology, nitrogen content, or biomass. Across a species’ range, understanding how these characteristics vary in response to their current environments lays the groundwork for predicting their responses to novel environments. We investigated northern red oak (Quercus rubra L.) fine root trait and biomass responses to growing season temperature and precipitation, and soil properties. Methods: We sampled soil and Q. rubra …


Antioxidant Potential Of A Non-Heme Iron Complex: Mitigating H2O2-Induced Cellular Damage, Magy A. Mekhail, David M. Freire, Cameron Bowers, Grant Elam, Nora Del Bosque, Hannah K. Pyle, Sarah K. Dunn, Spencer Lanyon, Timothy J. Hubin, Giridhar Akkaraju, Kayla N. Green Jun 2026

Antioxidant Potential Of A Non-Heme Iron Complex: Mitigating H2O2-Induced Cellular Damage, Magy A. Mekhail, David M. Freire, Cameron Bowers, Grant Elam, Nora Del Bosque, Hannah K. Pyle, Sarah K. Dunn, Spencer Lanyon, Timothy J. Hubin, Giridhar Akkaraju, Kayla N. Green

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Iron complexes with antioxidant activity have garnered significant interest for both general and medical applications, yet few studies have yielded functional nonheme iron-based mimics for H2O2 mitigation in aqueous environments. Here, we investigate water-soluble nonheme iron complexes derived from 12-membered pyridinophanes (CF3PyN3, PyN3, NMe2PyN3, and Py2N2) to evaluate the impact of ligand scaffolds on H2O2 decomposition activity both in vitro and in a cellular model. Speciation and kinetic analyses reveal that both electronic and geometric modulation of the macrocyclic …