Life Sciences Commons^™

Structure-Function Investigation Of Proteins Involved In Cellulose Biosynthesis By Escherichia Coli, Thomas Brenner

Theses and Dissertations (Comprehensive)

Bacteria thrive within multicellular communities called biofilms consisting of a self-produced matrix. Biofilm matrices improve bacterial adherence to surfaces while creating a barrier from host immune responses, disinfectants, antibiotics and other environmental factors. Persistent colonization by the widely distributed pathogens, Escherichia coli and Salmonella spp., has been linked to production of biofilms composed of the exopolysaccharide cellulose. Cellulose-containing biofilms are also important to Acetobacter, Sarcina, Rhizobium and Agrobacterium species to form symbiotic and pathogenic interactions. In Enterobacteriaceae, two operons (bcsABZC and bcsEFG) are proposed to encode for proteins that form a cellulose biosynthetic complex that spans the …

The Effects Of A 12-Week Custom Foot Orthotic Intervention On The Intrinsic Muscles Of The Foot, And Dynamic Stability During Unexpected Gait Termination In Healthy Young Adults, Katrina Protopapas

Theses and Dissertations (Comprehensive)

Introduction: Custom-made foot orthotics (CFO’s) are a commonly prescribed intervention to help individuals that are suffering from foot pain and foot disorders. However, the mechanisms of CFO’s are still poorly understood and are not well known. With the plantar intrinsic muscles of the foot being in direct contact with the CFO, it puts these structures at risk for disuse muscle atrophy as a result of being offloaded. Therefore, the purpose of the current study was to determine the effect of a 12-week custom-made foot orthotic intervention on the intrinsic muscles of the foot and dynamic stability during unexpected gait termination. …

Influence Of Topography And Moisture And Nutrient Availability On Green Alder Function On The Low Arctic Tundra, Nt, Katherine Louise Black Ms., Jennifer Lynn Baltzer Dr.

Theses and Dissertations (Comprehensive)

The Arctic has warmed by at least 3^°C over the past 50 years and this rapid warming is expected to continue. Climate warming is driving the proliferation of shrubs across the tundra biome with implications for energy balance, climate, hydrology, nutrient cycling, and biodiversity. Changes in tundra plant water use attributable to shrub expansion are predicted to increase evapotranspirative water loss which may amplify local warming and reduce run-off. However, little is known about the extent to which shrubs will enhance evapotranspirative water loss in these systems. Direct measures of shrub water use are needed to accurately predict …

A Marine Heat Wave Drives Massive Losses From The World’S Largest Seagrass Carbon Stocks, Ariane Arias-Ortiz, Oscar Serrano, Pere Masqué, Paul Lavery, Ute A. Mueller, Gary A, Kendrick, Mohammad Rozaimi, Alba Esteban, James W, Fourqurean, Núrià N. Marbà, Miquel-Angel Mateo, Kathy Murray, Michael Rule, Carlos M. Duarte

Research Datasets

The database compiles published data (in Arias-Ortiz et al., 2018) on biogeochemical characteristics (density, organic carbon, stable carbon isotopes and sediment grain size) of soils underneath seagrass meadows in Shark Bay, Western Australia. Pb-210 concentrations of the first 20 cm are included along with C-14 raw ages. The dataset compiles data for a total of 49 cores, 25 - 300 cm-long (11 cores with Pb-210 and C-14 data).

The Validity Of Height As A Determinant Of Takeoff Distance To Hurdle One In Ncaa Division Iii Hurdlers 2017, Phillip Wiltshire

Master's Theses

The purpose of this study was to determine if the relationships between the height of a hurdler and the takeoff distance to the first hurdle for men and women as reported by Frye (2008) were valid for NCAA Division III 100 m and 110 m hurdlers. With no specific research reported to validate Frye’s data, it is unclear how the suggested takeoff distances were developed. It is also unclear whether hurdler height or another factor is the main determinant of takeoff distance for the high hurdlers. Participants in this study were three female 100 m hurdlers and four male 110 …

Effects Of Group Selection With Yellow Birch (Betula Alleghaniensis) Retention On The Understory And Sapling Layer In Northern Hardwood Forests, Megan A. Petras O'Neil

Dissertations, Master's Theses and Master's Reports

Gap partitioning theory predicts that changes in microenvironment conditions found within a forest opening promote diversity in forest ecosystems. Under this theory we would expect to see variations in tree and understory diversity throughout and surrounding a forest opening. In order to test this theory, we examined manmade openings with legacy-tree retention in a northern hardwood forest located in the Upper Peninsula of Michigan. This work is part of an ongoing study that was started in 2003 with the creation of 49 openings centered on a reserve dominant or co-dominant yellow birch (Betula alleghaniensis Britt.). The primary objective of …

Impacts To Biodiversity And Ecosystem Services From Bioenergy Development: A Pan American Experience, Colin Phifer

Dissertations, Master's Theses and Master's Reports

As part of a broader project that evaluated the social and ecological sustainability of bioenergy, I studied the effects of bioenergy associated land-use change and management on native bees and birds in two bioenergy-producing countries, the United States and Argentina. In Argentina, I worked in Entre Ríos province where eucalyptus (Eucalyptus spp.) plantations are being planted. These fast-growing trees are replacing pasture and annual crops, the current dominant land use. I surveyed for native bees and birds in pastures/annual crops and large-scale eucalyptus plantations, as well as mixed-use farms and native espinal savannas. Both birds and bees declined in …

The Role Of The Seagrass Leaf Microbiome In Assisting Nitrogen Uptake By The Western Australian Seagrass, Posidonia Sinuosa, Flavia Tarquinio

Theses: Doctorates and Masters

Microorganisms play a key role in facilitating the cycling of several elements in coastal environments, including nitrogen (N). N is a key component for maintaining high seagrass productivity and is often the limiting nutrient in marine environments. Seagrasses harbour an abundant and diverse microbial community (the ‘microbiome’), however their ecological and functional roles related to the seagrass host are still poorly understood, in particular regarding N cycling. Microorganisms capable of mineralising dissolved organic nitrogen (DON) may play a pivotal role in enhancing N availability in coastal environments such as seagrass meadows. Thus, the overall aim of my thesis was to …

Identification Of Multiple Functional Receptors For Tyramine On An Insect Secretory Epithelium, Haiying Zhang, Edward M. Blumenthal

Biological Sciences Faculty Research and Publications

The biogenic amine tyramine (TA) regulates many aspects of invertebrate physiology and development. Although three TA receptor subtypes have been identified (TAR1-3), specific receptors have not been linked to physiological responses in native tissue. In the Malpighian (renal) tubule of Drosophila melanogaster, TA activates a transepithelial chloride conductance, resulting in diuresis and depolarization of the transepithelial potential. In the current work, mutation or RNAi-mediated knockdown in the stellate cells of the tubule of TAR2 (tyrR, CG7431) resulted in a dramatic reduction, but not elimination, of the TA-mediated depolarization. Mutation or knockdown of TAR3 (tyrRII, …

The Outcome Of Elisa For Antiphosphatidylethanolamine Antibodies Is Dependent On The Composition Of Phosphatidylethanolamine, Songwang Hou, Paul E. Harper, Nathalie Bardin, Ming Zhao

University Faculty Publications and Creative Works

Objective The presence of circulating autoantibodies against phosphatidylethanolamine (PE) has been shown to be positively associated with symptoms of antiphospholipid syndromes (APS). However, the current ELISA-based tests for antiphosphatidylethanolamine (aPE) antibodies remain inconsistent and controversial. The term PE refers to a collection of phospholipids that have phosphorylethanolamine head group as a common structural feature, but can vary in fatty acids with diverse physicochemical properties. The present study was to investigate, using synthetic positionally symmetrical PE species as a model system, the impact of PE structural variations on aPE ELISA. Methods Single and combinations of synthetic PE species, including 16:0 (fatty …

Complete Genome Sequences Of 38 Gordonia Sp. Bacteriophages, Welkin H. Pope, Matthew T. Montgomery, Randall J. Dejong

University Faculty Publications and Creative Works

We report here the genome sequences of 38 newly isolated bacteriophages using Gordonia terrae 3612 (ATCC 25594) and Gordonia neofelifaecis NRRL59395 as bacterial hosts. All of the phages are double-stranded DNA (dsDNA) tail phages with siphoviral morphologies, with genome sizes ranging from 17,118 bp to 93,843 bp and spanning considerable nucleotide sequence diversity.

Changes In Appetite, Energy Intake, Body Composition And Circulating Ghrelin Constituents During An Incremental Trekking Ascent To High Altitude, Jamie Matu, John O'Hara, Neil Hill, Sarah B. Clarke, Christopher Boos, Caroline Newman, David Holdsworth, Theocharis Ispoglou, Lauren Duckworth, David Woods, Adrian Mellor, Kevin Deighton

Journal Articles

No abstract provided.

Knowledge Of Forest Ecosystem Among Forest Guards In Southern Nigeria, Norris I. Erhabor

Journal of Bioresource Management

Undertaken study assessed the level of ecological knowledge among forest guards in southern Nigeria. Descriptive survey research design was used for study. A structured questionnaire was administered to 60 respondents that are employed by forest guards system. Central question the study attempts to answer is the level of knowledge of the ecosystem among forest guards. Analysis of data revealed that forest guards have inadequate knowledge of ecosystem. Calculated mean score of respondents was 14.25. This mean score is lower than theoretical mean of 16 (80%). A binomial test revealed that fairly high number of respondents had inadequate knowledge of …

Weaning Strategies In Beef Cattle To Reduce Stress, Matthew Garcia, Kerry Rood, Clark Israelsen

All Current Publications

This fact sheet considers weaning strategies for beef cattle, including the advantages and disadvantages of fenceline weaning, and twostep weaning.

Synthesis And Characterization Of Amide Linked Triazolyl Glycolipids As Molecular Hydrogelators And Organogelators, Guijun Wang, Anji Chen, Hari P. R. Mangunuru, Jayasudhan Reddy Yerabolu

Chemistry & Biochemistry Faculty Publications

Carbohydrate based small molecular gelators are important classes of compounds which can form useful soft materials with many practical applications. Although many different types of effective gelators have been reported, the rational design of a molecular hydrogelator is still challenging. In this research, we combined the structural features of two classes of monosaccharide based molecular gelators and obtained a new class of glycolipids that can function as molecular gelators. These new compounds were synthesized by introducing a triazole functional group to a protected 2-glucosamine through Cu(i) catalyzed azide/alkyne cycloaddition reaction (CuAAC). A series of eighteen new glycolipids containing 4,6-O …

Evidence Of Chemical Camouflage In Pirate Perch (Aphredoderus Sayanus); Avoiding Detection By Colonizing/Ovipositing Prey Through Chemical Crypsis, Tyler Breech

Electronic Theses and Dissertations

No abstract provided.

Effect Of Body Image Awareness Program On Weight Satisfaction In Ncaa Division I Female Athletes, Derionne Brooks

Electronic Theses and Dissertations

No abstract provided.

Contribution Of Specific Amino Acids To Calcium-Dependent Dimerization Of Epithelial Cadherin, Xiaoyun Z. Howard

Electronic Theses and Dissertations

Cadherins are calcium-dependent molecules that play essential roles in embryonic development, tissue morphogenesis, and cancer progression. Epithelial (E-) cadherin is present at adherens junctions, structures that are important for maintaining tissue integrity. At adherens junctions, cell–cell adhesion complexes are formed as clusters of cadherin dimers through strand-swapped dimerization between two interacting protomers from adherent cells. Recently, a number of studies of dimer formation of E-cadherin supported a two-step adhesive binding model. First, an inter-molecular initial encounter complex (X-dimer) forms at the linker region of the first two extracellular domains, EC1 and EC2. Next, strand-swap dimerization occurs via exchange or ''swap'' …

Nucleic Acid Clamp-Mediated Transcriptional And Translational Modulation Of Oncogenes Via The Stabilization Of G-Quadruplexes, Taisen Hao

Electronic Theses and Dissertations

G-quadruplexes (G4s) are non-canonical secondary nucleic acid structures that exit in various biologically important regions within a cell, such as the promoters of DNA, 5’-Untranslated regions (UTR) of mRNA and telomeres. These secondary structures tend to cluster around 1 kb from transcriptional start sites across all human chromosomes in addition to at telomeric regions. Extensive efforts from many research groups have been dedicated to targeting G4s in oncogenic promoters, telomeres, and 5’-UTRs with traditional small molecules. However, most small molecules recognizing multiple G4s raising potential issues in terms of utilizing these small molecules clinically. Therefore, we developed a novel nucleic …

Regulation Of The Kras Promoter In Pancreatic Cancer By Proteins And Small Molecules, Harshul Batra

Electronic Theses and Dissertations

DNA-binding proteins play a pivotal role in cell biology. The major class of DNA-binding proteins are transcription factors (TFs). TFs are central to almost every fundamental cellular process such as cell development, differentiation, cell growth, and gene expression. They account for 10% of the genes in eukaryotes. In mammals, more than 700 TFs are identified to be DNA-binding TFs. They bind to the TF binding sites (TFBSs) in the genome and regulate the expression of their target genes. kRAS is a proto-oncogene with intrinsic GTPase activity, that contributes to cell proliferation, division, and apoptosis. kRAS mutations are observed in >95% …

Invasion Routes And Evolution Of Brook Stickleback (Culaea Inconstans) In Eastern Washington, Samuel Gunselman

EWU Masters Thesis Collection

Chapter 1. Invasive species pose a serious threat to native ecosystems. In eastern Washington brook stickleback (Culaea inconstans) is an important invasive species, especially at Turnbull National Wildlife Refuge, where they compete with waterfowl for food resources and are associated with declines in habitat quality. Understanding the invasion routes of this invasive species may help managers implement strategies to prevent further spread and mitigate damages caused by these fish. The objectives of this study were to identify the most likely point of brook stickleback invasion, to determine their most likely invasion routes, and to investigate landscape features and processes that …

The Acute Effect Vibrating Foam Rollers Have On The Lower Extremities' Ability To Produce Power, Jared L. Klingenberg

EWU Masters Thesis Collection

The purpose of this study was to examine the effect that vibration through self-myofascial release in combination with a dynamic stretch routine had on maximum power output. Twenty-one collegiate volleyball athletes agreed to participate in the study. The design was a randomized cross-over design in which all participants served as their own control by participating in all three interventions, which consisted of; a) dynamic stretch routine (DS), b) non-vibrating foam rolling combined with dynamic stretch routine (NVFR), and c) vibrating foam rolling combined with dynamic stretch routine (VFR). The foam rolling protocol consisted of rolling each limb bilaterally for 30 …

Instructional Strategies That Promote Cultural Competence In Nutrition And Dietetics Education, Cecile Adkins

Walden Dissertations and Doctoral Studies

Changing demographics in the United States to a minority-majority culture require health care professionals who are culturally competent to provide appropriate care to patients. In a university in the Mid-Atlantic region of the United States, a gap existed between student education and the culturally competent professional practice of entry-level registered dietitians. Using Freire's notion of social justice and Vygotsky's constructivist theory as the conceptual frameworks, the purpose of this study was to explore educators' instructional strategies that promoted cultural competence in nutrition and dietetics education. The research questions examined how educators described the instructional practices they employed to promote cultural …

The Effects Of Female Genital Mutilation On Women Of Sierra Leone, Nenneh Kalokoh Kalokoh

Walden Dissertations and Doctoral Studies

Female genital mutilation or cutting (FGM/C), a common practice among Sierra Leonean women, carries significant psychological and physical risks. Prior to this study, a substantial need existed for inquiry of the experiences and belief systems within this cultural group to better understand the effects of FGM/C on women and girls. Guided by feminist theory and the theory of cultural relativism, the goal of this phenomenological study was to review the cultural perspectives and experiences of Sierra Leonean women who underwent FGM/C to investigate their concerns about safety and their perceptions of the practices. In addition, this study explored concerns among …

Defense And Prosecuting Attorney Perceptions Of Facility Dogs In The Courtroom, Kristy Melissa Donaldson

Walden Dissertations and Doctoral Studies

Attorneys must obtain evidence for purporting their position on a court case. Many factors are considered when working with witness testimony such as age, development, narrative recall, and retraumatization. Research has detailed the hardship of obtaining evidence during witness testimony and the importance of support systems to help with the process. Existing research illustrates the stressors caused by the court processes, witness fear, and difficulty for attorneys to ascertain testimony. Therapeutic animals have been well researched and shown to have an important support role in many professions and situations. Additionally, much has been written regarding the use of varying types …

Assessing For Awareness And Knowledge Regarding Diabetes In Pre-Diabetes Obese Patients, Remona Lysa Brown

Walden Dissertations and Doctoral Studies

Over the past few decades, there has been an increase in prevalence of diabetes in the United States. Prevention of diabetes and improving patients' knowledge and awareness of diabetes are crucial for healthcare providers. Using the Health Belief Model (HBM) as a theoretical foundation, the student used the National Diabetes Prevention Program (NDPP) from the Centers for Disease Control and Prevention (CDC), to improve awareness and knowledge of diabetes among obese individuals with prediabetes. The key research question of this project was to determine whether the patients' diabetes knowledge and awareness improved after the NDPP program. A convenience sample of …

Predictors Of Compliance With The Food Safety And Inspection Service's Listeria Rule, 2012-2015, Amadou Samb

Walden Dissertations and Doctoral Studies

Since 1987, the Food Safety and Inspection Service (FSIS) has implemented a zero-tolerance policy for Listeria monocytogenes (Lm) in ready-to-eat (RTE) meat and poultry products, which culminated with the implementation of the Listeria rule in 2003. While researchers have extensively examined human listeriosis and its causative agent, Lm, there remained a significant gap in the current literature regarding how, singly or in combination, establishment size, RTE product type, Listeria alternative used, and FSIS district of production predict compliance with the Listeria rule. Therefore, the purpose of this quantitative study was to investigate the relationship between establishment size, RTE product type, …

Using Multi-Theory Model To Predict Low Salt Intake - Nigerian Adults With Hypertension, Christine Adekemi Dokun-Mowete

Walden Dissertations and Doctoral Studies

Hypertension is a chronic non-communicable disease and a major risk factor for cardiovascular diseases, renal malfunction, disability, and premature death. One of the public health recommendations for the management of hypertension is the reduction of sodium/salt intake. There is need to develop and implement new evidence-based theoretical interventions to initiate and sustain behavior change in health education and promotion. Therefore, the quantitative cross-sectional method and design was used to investigate the adequacy of multi-theory model (MTM) constructs for the initiation and the sustenance of low sodium/salt intake behavior in hypertensive Nigerian adults. In addition, the impact of the MTM (initiation) …

Development Of An Educational Program To Obtain And Maintain Healthy Weights Among 4th And 5th Grade Students, Stephanie Dean Black

Walden Dissertations and Doctoral Studies

Development of an Educational Program to Obtain and Maintain Healthy Weights Among 4th and 5th Grade Students

By

Stephanie D. Black

MSN, University of Phoenix, 2008

BSN, Southwest Baptist University, 2006

ASN, Southwest Baptist University, 2003

Capstone Project Submitted in Partial Fulfillment

of the Requirements for the Degree of

Doctor of Nursing Practice

Walden University

October 2017

Obesity is prevalent in schoolchildren and increases risk of chronic diseases throughout the lifespan. Strategies are needed to address this growing health problem. Education in elementary schools targeting the topics of nutritional choices and prevention of obesity, particularly in 4th-5th grade population, is …

Results Of The 2016 Indianapolis Biodiversity Survey, Marion County, Indiana, Jeffrey D. Holland, Rebecca W. Dolan, Jeremy J. Sheets, Michael S. Finkler, Brant E. Fisher, Roger L. Hedge, Tom Swinford, Nick Harby, Robert P. Jean, Megan K. Martin, Bill Mcknight, Marc Milne, Kirk Roth, Paul Rothrock, Carl Strang

Scholarship and Professional Work - LAS

Surprising biodiversity can be found in cities, but urban habitats are understudied. We report on a bioblitz conducted primarily within a 24-hr period on September 16 and 17, 2016 in Indianapolis, Indiana, USA. The event focused on stretches of three waterways and their associated riparian habitat: Fall Creek (20.6 ha; 51 acres), Pleasant Run (23.5 ha; 58 acres), and Pogue’s Run (27.1 ha; 67 acres). Over 75 scientists, naturalists, students, and citizen volunteers comprised 14 different taxonomic teams. Five hundred ninety taxa were documented despite the rainy conditions. A brief summary of the methods and findings are presented here. Detailed …