Life Sciences Commons^™

Effect Of Exposure Of Raw264.7 Macrophages To Salmonella Typhimurium Components On Cell Viability, Cytoskeleton Re-Arrangement And Cytokine Secretion, Khalid Abdullah Alyahya

Browse all Theses and Dissertations

Innate immune system plays an important role in individual's protection against pathogens and in activation of adaptive immune system. Utilizing RAW 264.7 murine macrophages as an innate immune response representative in this study, we analyzed the effect of invasive pathogen's components (e.g. flagellin) on the arrangement of macrophage's cytoskeleton, on viability of immune cells and on secretion of pro-inflammatory and anti-inflammatory cytokines and on fluorescence intensity of cytoskeleton after rearrangement. Additionally, we studied the similarity and differences between bacterial (Salmonella typhimurium) and synthetic TLR4 agonist (synthetic lipid-A) on viability, fluorescence intensity, cytokine secretion, and cytoskeleton rearrangements. Similarly, we studied the …

Investigating The Role Of An Sk Channel Activator On Survival And Motor Function In The Sod1-G93a, Als Mouse Model, Matthew Thomas Dancy

Browse all Theses and Dissertations

Amyotrophic Lateral Sclerosis (ALS) is a fatal, adult-onset progressive degenerative motor neuron disease that is characterized by muscle atrophy and weakness due to the loss of upper and lower motor neurons. Average survival time for individuals diagnosed with the disease is three to five years; currently there is no cure and only one drug approved by the Food and Administration (FDA). Scientists have proposed various theories in order to solve the mystery which surrounds ALS. One of these theories hypothesizes how hyperexcitability and excitotoxicity leads to the death of motor neurons. In this study, we will address ways of combatting …

A Novel Molecular Relationship Between Parn And Pld That, When Deregulated, Contributes To The Aggressive Phenotype Of Breast Cancer Cell Lines., Taylor Elaine Miller

Browse all Theses and Dissertations

The removal of mRNA transcript poly(A) tails by 3'-5' exonucleases is the rate-limiting step for controlled mRNA decay in eukaryotes. Poly(A)-specific ribonuclease (PARN) is one such exonuclease that degrades poly(A) tails, and although its in vitro activity is well-characterized, PARN’s patho-physiological roles in the cell are not well understood. Prior studies have found a possible role for PARN in cancer, in that PARN expression levels in human breast cancer tissues are often decreased compared to normal control tissues. Indeed, data mined from the ONCOMINE cancer array database showed that PARN is downregulated in patient invasive breast carcinoma samples compared to …

Antimicrobial Activity Of Fractionated Borohydride-Capped And Electrochemical Colloidal Silver, Marjorie M. Markopoulos

Browse all Theses and Dissertations

Silver nanoparticles (AgNPs) and ionic silver (Ag+) are known to be broad-spectrum antimicrobial agents. Recent studies show these agents may be an alternative to the most widely used drinking water disinfectant, chlorine. Chlorine is a toxic industrial chemical with a lethal concentration of 430 ppm after 30 minutes. Additionally, chlorine can react with naturally occurring materials to produce a number of disinfection byproducts such as chloroform and trihalomethanes. Some of these byproducts pose cancer risks in addition to other negative impacts to human health. These would be eliminated with the use of Ag+ or AgNPs. The main goal of this …

Living With Fragile X Syndrome: Occupations As An Outcome Measure In A Clinical Trial, Kaylee E. Gothelf, Ty Duong, Ana Baldinger, Theresa Chase

Student Research Posters

The purpose of this study was to evaluate the use of semi-structured interviews as an outcome measure in a clinical trial for children with fragile X syndrome (FXS) ages 2-6. This qualitative approach was used to analyze twenty-five interviews of parents with children in a double-blind medication trial - sertraline or placebo. The aim was to assess occupational improvements in their child that may not have been detected with the use quantitative outcome measures alone. Results showed greater improvements in the sertraline group in areas of behavior, social participation, sensory-related behaviors, receptive language, education, family impact and therapeutic strategies. Our …

An Annotated Checklist Of The Vascular Plants Of Oklahoma, James P. Smith Jr

Botanical Studies

No abstract provided.

Can Peer Education Improve Beliefs, Knowledge, Motivation And Intention To Engage In Falls Prevention Amongst Community-Dwelling Older Adults?, Linda Khong, Richard G. Berlach, Keith D. Hill, Anne-Marie Hill

IHR Papers and Journal Articles

The aim of the study was to evaluate the effectiveness of delivering a contemporary peer-led falls prevention education presentation on community-dwelling older adults’ beliefs, knowledge, motivation and intention to engage in falls prevention strategies. A two-group quasi-experimental pre-test–post-test study using a convenience sample was conducted. A new falls prevention training package for peer educators was developed, drawing on contemporary adult learning and behaviour change principles. A 1-h presentation was delivered to community-dwelling older adults by peer educators trained with the new package (intervention group). Control group participants received an existing, 1-h falls prevention presentation by trained peer educators who had …

How Older Adults Would Like Falls Prevention Information Delivered: Fresh Insights From A World Cafe Forum, Linda Khong, Caroline Bulsara, Keith D. Hill, Anne-Marie Hill

IHR Papers and Journal Articles

Translation of falls prevention evidence into practice is problematic. Understanding older adults’ views about falls prevention information could enhance delivery of falls prevention, resulting in better engagement and uptake of recommended activities. The aim of this study was to examine the views and preferences of community-dwelling older adults about seeking and receiving falls prevention information. A community forum using a modified World Café approach was conducted. Participants discussed five topic areas in small groups, under the guidance of table facilitators. Perspectives were captured on paper. Thematic analysis was conducted to identify factors that influence participants’ engagement and uptake of information. …

The Neonatal Anti-Viral Response Fails To Control Measles Virus Spread In Neurons Despite Interferon-Gamma Expression And A Th1-Like Cytokine Profile, Priya Ganesan

Electronic Theses and Dissertations

Neonates are highly susceptible to infections in the central nervous system (CNS) and have a greater risk of viral infections and encephalopathies. Neurotropic viral infections can lead to blindness, hearing loss and neurological deficiencies such as cognitive impairment, epilepsy, and even death in the neonatal and pediatric populations. Viral infections also are hypothesized to indirectly contribute to neurodegenerative and neuropsychiatric diseases such as Schizophrenia and Parkinson’s disease later in life due to early neuronal damage or stress. Many diverse viruses are capable of invading the neonatal CNS including Borna Disease Virus, Coxsackievirus (CV), Herpes simplex viruses (HSV), and measles virus. …

Neuronal Gq Structures In Neurodegeneration, Damian S. Mcaninch

Electronic Theses and Dissertations

This study investigates protein nucleic acid interactions between various proteins and G quadruplex (GQ) forming messenger RNAs (mRNAs) in human neurological disorders. GQ structures are formed in DNA/RNA, when four guanine residues form planar tetrads stabilized by Hoogsteen base pairing, that stack forming a GQ structure stabilized by potassium ions. These GQ structures are targeted by the arginine-glycine-glycine (RGG) repeat domain containing RNA-binding domain.

Three RGG domain containing RNA-binding proteins, all of which have been implicated in neurological disorders, and their interactions with GQ forming mRNAs, were investigated in this study: fused in sarcoma (FUS), fragile X mental retardation protein …

Distribution Changes, Genetic Population Structure, And A Novel Environmental Dna (Edna) Detection Method For Darters (Subgenus Nothonotus) In The Upper Ohio River Watershed, Anthony S. Honick

Electronic Theses and Dissertations

In the upper Ohio River watershed three species of small-bodied benthic fish the Bluebreast Darter, Etheostoma (Nothonotus) camurum (Cope), the Tippecanoe Darter, Etheostoma (Nothonotus) tippecanoe Jordan and Evermann, and the Spotted Darter, Etheostoma (Nothonotus) maculatum Kirtland previously existed in disjunct distributions due to poor water quality and habitat degradation. Signs of recovery indicated that these species were moving from areas of refugia into the deeper mainstem waters of the Allegheny and Ohio rivers and expanding their distributions. To provide information for the proper conservation management of these species this dissertation was divided into three stages: 1) distribution records were updated …

Accuracy And Repeatability Of Single-Pose Calibration Of Inertial Measurement Units For Whole-Body Motion Analysis, Xavier Robert-Lachaine, Hakim Mecheri, Christian Larue, André Plamondon

Études primaires

Portable inertial measurement units (IMUs) are suitable for motion analysis outside the laboratory. However, IMUs depend on the calibration of each body segment to measure human movement. Different calibration approaches have been developed for simplicity of use or similarity to laboratory motion analysis, but they have not been extensively examined. The main objective of the study was to determine the accuracy and repeatability of two common single-pose calibrations (N-pose and T-pose) under different conditions of placement (self-placement and passive placement), as well as their similarity to laboratory analysis based on anatomical landmarks. A further aim of the study was to …

Obesity And Obesity Shape Markedly Influence Spine Biomechanics: A Subject-Specific Risk Assessment Model, Farshid Ghezelbash, Aboulfazl Shirazi-Adl, André Plamondon, Navid Arjmand, Mohammad Parnianpour

Études primaires

Underlying mechanisms of obesity-related back pain remain unexplored. Thus, we aim to determine the effect of obesity and its shapes on the spinal loads and the associated risks of injury. Obesity shapes were initially constructed by principal component analysis based on datasets on 5852 obese individuals. Spinal loads, cycles to vertebral failure and trunk stability margin were estimated in a subject-specific trunk model taking account of personalized musculature, passive ligamentous spine, obesity shapes, segmental weights, spine kinematics and bone mineral density. Three obesity shapes (mean and extreme abdominal circumferences) at three body weights (BWs) of 86, 98 and 109 kg …

The Effect Of An 8-Week Stabilization Exercise Program On The Lumbopelvic Rhythm And Flexion-Relaxation Phenomenon, Ali Shahvarpour, Sharon M. Henry, Richard Preuss, Hakim Mecheri, Christian Larivière

Études primaires

Background

Lumbar stabilization exercise programs should normalize the aberrant movements patterns often observed in patients with low back pain. This study aimed to determine the effect of an 8-week lumbar stabilization program on EMG/kinematics measures of the aberrant movement patterns in such patients. A secondary goal was to assess the 8-week test-retest reliability of these measures.

Methods

The patients followed an 8-week lumbar stabilization program while no intervention was carried out on the controls. Before and after this period, kinematics of the spine along with the EMG of paraspinal muscles were recorded during trunk maximal flexion-extension. ANOVAs tested the effect …

2017 Weed Science Research Report – Blue Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2017. It includes work on corn, soybean and the management of several weeds in North Dakota.

2017 Weed Science Research Report – Yellow Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2017. It includes work on several minor crops and the management of several weeds in North Dakota.

2017 Weed Science Research Report – Gold Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2017. It includes work on sugarbeet and the management of several weeds in North Dakota.

2017 Weed Science Research Report – Green Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2017. It includes work on small grains and the management of several weeds in North Dakota.

2017 Weed Science Research Report – Pink Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2017. It includes work on leafy spurge in North Dakota.

2017 Weed Science Research Report – Tan Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2017. It includes work on adjuvants, spray quality and the management of several weeds in North Dakota.

Applying Theory Of Constraints To Timber Harvesting: A Case Study From The Northeast Usa, Matthew Kelly, René H. Germain

Michigan Tech Publications, Part 1

Logging firms are a critical link in wood supply chains, connecting forest landowners with markets for wood products. Improving operational planning can benefit individual logging firms as well as the larger wood supply chain in which they operate. Applying concepts from Theory of Constraints (TOC) to timber harvesting may help achieve greater predictability and efficiency when planning harvest operations. However, examples that demonstrate how TOC can improve logging operations are lacking. This study focuses on the analysis of production and activity data collected during the harvest of a temperate mixed hardwood forest in the Northeast United States using a chainsaw-forwarder …

Crops In Silico: Generating Virtual Crops Using An Integrative And Multi-Scale Modeling Platform, Amy Marshall-Colon, Stephen P Long, Douglas K Allen, Gabrielle Allen, Daniel A Beard, Bedrich Benes, Susanne Von Caemmerer, A J Christensen, Donna J Cox, John C Hart, Peter M Hirst, Kavya Kannan, Daniel S Katz, Jonathan P Lynch, Andrew J Millar, Balaji Panneerselvam, Nathan D Price, Przemyslaw Prusinkiewicz, David Raila, Rachel G Shekar, Stuti Shrivastava, Diwakar Shukla, Venkatraman Srinivasan, Mark Stitt, Matthew J Turk, Eberhard O Voit, Yu Wang, Xinyou Yin, Xin-Guang Zhu

Articles, Abstracts, and Reports

Multi-scale models can facilitate whole plant simulations by linking gene networks, protein synthesis, metabolic pathways, physiology, and growth. Whole plant models can be further integrated with ecosystem, weather, and climate models to predict how various interactions respond to environmental perturbations. These models have the potential to fill in missing mechanistic details and generate new hypotheses to prioritize directed engineering efforts. Outcomes will potentially accelerate improvement of crop yield, sustainability, and increase future food security. It is time for a paradigm shift in plant modeling, from largely isolated efforts to a connected community that takes advantage of advances in high performance …

The Rs16906252:C>T Snp Is Not Associated With Increased Overall Survival Or Temozolomide Response In A Han-Chinese Glioma Cohort., Kuo-Chen Wei, Chia-Yuan Chen, Li-Ying Feng, Wei-Tzu Huang, Chia-Hua Chen, Peng-Wei Hsu, Kai Wang, Leroy Hood, Leslie Y Chen

Articles, Abstracts, and Reports

The methylation status of O-6-methylguanine-DNA methyltransferase (MGMT) is associated with the prognosis in gliomas and in other cancers. Recent studies showed that rs16906252, an SNP in the MGMT promoter, is associated with promoter methylation and is a predictor of the overall survival time (OST) and the response to temozolomide (TMZ) treatment. However, these findings haven't been systematically investigated in the Han-Chinese population. We analyzed the relevance between rs16906252 polymorphisms, the MGMT methylation status, and the OST in 72 Han-Chinese gliomas patients. The MGMT promoter methylation was measured by bisulfite conversion followed by pyro-sequencing, while rs16906252 was measured by restriction endonuclease …

An Indicator Cell Assay For Blood-Based Diagnostics., Samuel A Danziger, Leslie R Miller, Karanbir Singh, G Adam Whitney, Elaine R Peskind, Ge Li, Robert J Lipshutz, John D Aitchison, Jennifer J Smith

Articles, Abstracts, and Reports

We have established proof of principle for the Indicator Cell Assay Platform™ (iCAP™), a broadly applicable tool for blood-based diagnostics that uses specifically-selected, standardized cells as biosensors, relying on their innate ability to integrate and respond to diverse signals present in patients' blood. To develop an assay, indicator cells are exposed in vitro to serum from case or control subjects and their global differential response patterns are used to train reliable, disease classifiers based on a small number of features. In a feasibility study, the iCAP detected pre-symptomatic disease in a murine model of amyotrophic lateral sclerosis (ALS) with 94% …

Differences Between The Genomes Of Lymphoblastoid Cell Lines And Blood-Derived Samples., Lena M Joesch-Cohen, Gustavo Glusman

Articles, Abstracts, and Reports

Lymphoblastoid cell lines (LCLs) represent a convenient research tool for expanding the amount of biologic material available from an individual. LCLs are commonly used as reference materials, most notably from the Genome in a Bottle Consortium. However, the question remains how faithfully LCL-derived genome assemblies represent the germline genome of the donor individual as compared to the genome assemblies derived from peripheral blood mononuclear cells. We present an in-depth comparison of a large collection of LCL- and peripheral blood mononuclear cell-derived genomes in terms of distributions of coverage and copy number alterations. We found significant differences in the depth of …

Distribution Of The North American Porcupine (Erethizon Dorsatum) In Northern California, Cara L. Appel, William J. Zielinski, Frederick V. Schlexer, Richard Callas, Tim Bean

Open Educational Resources

Western Wildlife 4:17–28, 2017: The North American Porcupine (Erethizon dorsatum) is one of the most widely distributed mammals in North America, but recent reports have suggested declines in parts of its range in the West. In California, little is known about the historical or current status of the porcupine, and maps of its distribution conflict considerably. Nevertheless, the species is of interest to natural resource managers. For much of the 1900s, foresters and others primarily treated porcupines as pests because of the undesirable damage they inflict feeding on trees and gnawing on manmade items in search of salt. …

Glaucous-Winged Gull Predation On Chicken Eggs Treated With A Uv-Blocking Agent, Athena T. Smith

Master's Theses

Egg cannibalism plays a major role in the life histories of Glaucous-winged Gulls (Larus glaucescens) breeding at Protection Island, Washington. Gulls, along with other birds, visualize reflected ultraviolet (UV) light and I wondered if reflected UV light plays a role in determining which eggs are selected for cannibalization. Motivated by studies showing that tree-nesting bird eggs coated with a UV-blocking agent were less subject to predation than control eggs, I tested whether ground-nesting Glaucous-winged Gulls would preferentially predate control chicken eggs over those coated with a UV-blocking agent. Early during five mornings I formed artificial nests at randomly-determined …

Department Of Fisheries Annual Report To Parliament 2016/17, Government Of Western Australia Department Of Fisheries

Department of Fisheries Annual Reports

This annual report provides an overview of the Department and its operations for the financial year ended 30 June 2017. It has been prepared in accordance with the requirements of the Financial Management Act 2006, the Fish Resources Management Act 1994 and the Public Sector Commission’s Annual reporting framework – 2016/17 reporting year.

Goldfields Nullarbor Rangelands Biosecurity Association Inc. Activity Report 2016/17, Goldfields Nullarbor Rangelands Biosecurity Association Inc.

Recognised Biosecurity Group Annual Reports

The Goldfields Nullarbor Rangelands Biosecurity Association Inc. (GNRBA) is unique in rangeland management across Western Australia. It has a huge area to service and is working hard to provide a top class service to ratepayers. It is now well recognised as being a leader in the RBG’s in WA. The GNRBA Inc formally became operational on 1st July 2011. At this time it took over the APB Zone 9 operations.

Kimberley Rangelands Biosecurity Association Invasive Species Activity Report 2016/17, Kimberley Rangelands Biosecurity Association

Recognised Biosecurity Group Annual Reports

The Kimberley Rangelands Biosecurity Association (KRBA) has an annual Large Feral Herbivore culling program that includes feral donkeys, horses and camels with other animals such as water buffalo and pigs targeted when located. The program focusses primarily on donkeys, using the radio telemetry technique with horses and camels culled opportunistically during tracking. Currently there are 135 active collars throughout the region.