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Articles 117001 - 117030 of 256032

Full-Text Articles in Life Sciences

A Parapoxviral Virion Protein Targets The Retinoblastoma Protein To Inhibit Nf-Κb Signaling, Ponnuraj Nagendraprabhu, Sushil Khatiwada, Sabal Chaulagain, Gustavo A. Delhon, Daniel L. Rock Jan 2017

A Parapoxviral Virion Protein Targets The Retinoblastoma Protein To Inhibit Nf-Κb Signaling, Ponnuraj Nagendraprabhu, Sushil Khatiwada, Sabal Chaulagain, Gustavo A. Delhon, Daniel L. Rock

School of Veterinary and Biomedical Sciences: Faculty Publications

Poxviruses have evolved multiple strategies to subvert signaling by Nuclear Factor κB (NF-κB), a crucial regulator of host innate immune responses. Here, we describe an orf virus (ORFV) virion-associated protein, ORFV119, which inhibits NF-κB signaling very early in infection (≤30 min post infection). ORFV119 NF-κB inhibitory activity was found unimpaired upon translation inhibition, suggesting that virion ORFV119 alone is responsible for early interference in signaling. A C-terminal LxCxE motif in ORFV119 enabled the protein to interact with the retinoblastoma protein (pRb) a multifunctional protein best known for its tumor suppressor activity. Notably, experiments using a recombinant virus containing an ORFV119 …


Structural Studies Demonstrating A Bacteriophage-Like Replication Cycle Of The Eukaryote-Infecting Paramecium Bursaria Chlorella Virus-1, Elad Milrot, Eyal Shimoni, Katya Rechav, Tamar Unger, James L. Van Etten, Abraham Minsky Jan 2017

Structural Studies Demonstrating A Bacteriophage-Like Replication Cycle Of The Eukaryote-Infecting Paramecium Bursaria Chlorella Virus-1, Elad Milrot, Eyal Shimoni, Katya Rechav, Tamar Unger, James L. Van Etten, Abraham Minsky

James Van Etten Publications

A fundamental stage in viral infection is the internalization of viral genomes in host cells. Although extensively studied, the mechanisms and factors responsible for the genome internalization process remain poorly understood. Here we report our observations, derived from diverse imaging methods on genome internalization of the large dsDNA Paramecium bursaria chlorella virus-1 (PBCV-1). Our studies reveal that early infection stages of this eukaryotic- infecting virus occurs by a bacteriophage-like pathway, whereby PBCV-1 generates a hole in the host cell wall and ejects its dsDNA genome in a linear, base-pair-by-base-pair process, through a membrane tunnel generated by the fusion of the …


Bioactive Compounds From Ventilago Denticulata Willd., Wannapha Molee Jan 2017

Bioactive Compounds From Ventilago Denticulata Willd., Wannapha Molee

Chulalongkorn University Theses and Dissertations (Chula ETD)

New naphthalene derivatives (1 and 2) and a new isomer of ventilagolin (3), together with three known anthraquinones, including chrysophanol (4), physcin or emodin 3-methyl ether (5), and emodin (6), were isolated from vines of Ventilago denticulata. The isolated compounds exhibited cytotoxic activity against cancer cell lines (HuCCA-1, A549, HepG-2, MOLT-3) with IC50 values of 1.15-40.54 µg/mL. Compounds 1-3 exhibited weak antibacterial activity (MIC 200.0-400.0 µg/mL), while emodin (6) displayed moderate antibacterial activity with MIC 25.0 µg/mL. Compounds 1-3, 5, and 6 exhibited nitric oxide radical scavenging activities with IC50 232.0-604.3 µM and compounds 1 and 2 exhibited DPPH radical …


การยับยั้งการเจริญและการผลิตอะฟลาทอกซินของ Aspergillus Flavus ในไซเลจข้าวโพดโดยใช้ยีสต์และแล็กติกแอซิดแบคทีเรียปฏิปักษ์, ทรรศภรณ์ รุ่งชัยวัฒนกุล Jan 2017

การยับยั้งการเจริญและการผลิตอะฟลาทอกซินของ Aspergillus Flavus ในไซเลจข้าวโพดโดยใช้ยีสต์และแล็กติกแอซิดแบคทีเรียปฏิปักษ์, ทรรศภรณ์ รุ่งชัยวัฒนกุล

Chulalongkorn University Theses and Dissertations (Chula ETD)

วัตถุประสงค์ของงานวิจัยนี้เพื่อศึกษาความสามารถของยีสต์และแล็กติกแอซิดแบคทีเรียปฏิปักษ์ในการยับยั้งการเจริญและการผลิตอะฟลาทอกซินรวมของ Aspergillus flavus บนจานอาหารเลี้ยงเชื้อและในไซเลจข้าวโพด และเพื่อหาปริมาณความเข้มข้นและอัตราส่วนของแล็กติกแอซิดแบคทีเรียและยีสต์ผสมที่ให้ผลการยับยั้งสูงสุดในไซเลจข้าวโพด โดยเริ่มจากการทดสอบประสิทธิภาพของยีสต์ (Wickerhamomyces anomalus MSCU 0652 และ Kluyveromyces marxianus MSCU 0655) และแล็กติกแอซิดแบคทีเรีย (Lactobacillus casei AN2 และ Lactobacillus paracasei AN3) ในการยับยั้งการเจริญและการผลิตอะฟลาทอกซินของ A. flavus บนอาหารเลี้ยงเชื้อ พบว่า จุลินทรีย์ปฏิปักษ์ที่ใช้ทดสอบทั้งหมดให้ผลการยับยั้งการเจริญของราค่อนข้างต่ำ (10-20 เปอร์เซ็นต์) อย่างไรก็ตาม ยีสต์ผสมระหว่าง W. anomalus MSCU 0652 และ K. marxianus MSCU 0655 และ L. paracasei AN3 แบบเดี่ยวมีประสิทธิภาพในการลดการผลิตอะฟลาทอกซินรวมสูงสุด (43.25 และ 35.64 เปอร์เซ็นต์ ตามลำดับ) ดังนั้น จึงนำยีสต์ผสมและ L. paracasei AN3 มาทดสอบความสามารถในการยับยั้งการเจริญและการผลิตอะฟลาทอกซินรวมของ A. flavus ในไซเลจข้าวโพดปลอดเชื้อและไซเลจข้าวโพดร่วมกับจุลินทรีย์ประจำถิ่น พบว่า การใช้ L. paracasei AN3 ร่วมกับยีสต์ผสมระหว่าง W. anomalus MSCU 0652 และ K. marxianus MSCU 0655 สามารถยับยั้งการเจริญและลดปริมาณอะฟลาทอกซินรวมของราได้ดีทั้งในไซเลจข้าวโพดปลอดเชื้อและไซเลจข้าวโพดที่หมักร่วมกับจุลินทรีย์ประจำถิ่น จากนั้น เมื่อทดสอบความเข้มข้นและอัตราส่วนของ L. paracasei AN3 ร่วมกับยีสต์ผสมระหว่าง W. anomalus MSCU 0652 และ K. marxianus MSCU 0655 ที่เหมาะสม พบว่า การเติม L. paracasei AN3 ที่ความเข้มข้นมากกว่ายีสต์ผสม (10⁸:10⁶) …


การคัดกรองและการประยุกต์เครื่องหมายไมโครแซทเทลไลท์เพื่อการวิเคราะห์ความหลากหลายทางพันธุกรรมของนกกระเรียนพันธุ์ไทย Grus Antigone Sharpii Blanford, 1895 ในสภาพกรงเลี้ยง, รังสินี สันคม Jan 2017

การคัดกรองและการประยุกต์เครื่องหมายไมโครแซทเทลไลท์เพื่อการวิเคราะห์ความหลากหลายทางพันธุกรรมของนกกระเรียนพันธุ์ไทย Grus Antigone Sharpii Blanford, 1895 ในสภาพกรงเลี้ยง, รังสินี สันคม

Chulalongkorn University Theses and Dissertations (Chula ETD)

Grus antigone sharpii จัดเป็นนกกระเรียนที่เคยสูญพันธุ์ไปจากธรรมชาติของประเทศไทย การเพาะเลี้ยงและขยายพันธุ์นกกระเรียนในกรงเลี้ยงเป็นขั้นตอนสำคัญที่สามารถเพิ่มจำนวนประชากรเพื่อนำไปปล่อยคืนสู่ธรรมชาติในอนาคตได้ โดยสิ่งสำคัญที่ต้องคำนึงถึงคือความหลากหลายทางพันธุกรรม โดยเฉพาะอย่างยิ่งในกลุ่มของพ่อแม่พันธุ์ตั้งต้น ในการศึกษาครั้งนี้มีวัตถุประสงค์เพื่อคัดกรองเครื่องหมายไมโครแซทเทลไลท์จำนวน 28 ตำแหน่ง ซึ่งเป็นไพรเมอร์ที่เคยใช้ศึกษาในนกกระเรียน G. americana และนกกระเรียน Anthropoides paradisea เพื่อใช้วิเคราะห์ความหลากหลายทางพันธุกรรมของนกกระเรียนพันธุ์ไทยจากสวนสัตว์เปิดเขาเขียว (KKOZ, n = 11) และสถานีวิจัยเพาะเลี้ยงนกน้ำบางพระ (BB, n = 17) จังหวัดชลบุรี ผลการศึกษาพบว่าไพรเมอร์ทั้ง 28 คู่สามารถเพิ่มปริมาณไมโครแซทเทลไลท์ดีเอ็นเอของนกกระเรียนพันธุ์ไทยได้ แต่มีเพียง 22 คู่ที่แสดงความเป็น polymorphism และมีตำแหน่งไมโครแซทเทลไลท์ที่ปรากฏค่า linkage disequilibrium 8 ตำแหน่ง การเบี่ยงเบนออกจากสมดุล Hardy-Weinberg พบที่โลคัส Gram8 และ Gpa38 ซึ่งเป็นผลมาจาก null allele และการเพิ่มขึ้นของอัลลีลแบบ heterozygous ตามลำดับ จากการวิเคราะห์ค่าความหลากหลายทางพันธุกรรมพบว่า ค่าเฉลี่ยของ expected และ observed heterozygosity มีค่าเท่ากับ 0.63 และ 0.69 ค่า inbreeding coefficient มีค่าเท่ากับ -0.100 ซึ่งแสดงว่านกกระเรียนมีความหลากหลายทางพันธุกรรมค่อนข้างสูง และเกิด inbreeding น้อย นอกจากนี้ยังพบว่านกกระเรียนจาก KKOZ และ BB มีความแตกต่างทางพันธุกรรมต่ำ (FST = 0.035) และไม่มีการแยกออกเป็นกลุ่มประชากรย่อย (K = 1) จากผลการศึกษาครั้งนี้บ่งบอกได้ว่านกกระเรียนพันธุ์ไทยจาก 2 พื้นที่การศึกษาน่าจะเหมาะสมที่จะใช้เป็นพ่อแม่พันธุ์เพื่อเพิ่มจำนวนประชากรในกรงเลี้ยงให้มากขึ้น และสามารถนำไปปล่อยคืนสู่พื้นที่ชุ่มน้ำตามธรรมชาติในอนาคตได้


Differential Precipitation And Solubilisation Of Proteins, Barry J. Ryan, Gemma K. Kinsella Jan 2017

Differential Precipitation And Solubilisation Of Proteins, Barry J. Ryan, Gemma K. Kinsella

Books/Book Chapters/ Proceedings

Differential protein precipitation is a rapid and economical step in protein purification and is based on exploiting the inherent physicochemical properties of the polypeptide. Precipitation of recombinant proteins, lysed from the host cell, is commonly used to concentrate the protein of choice before further polishing steps with more selective purification columns (e.g., His-Tag, Size Exclusion, etc.). Recombinant proteins can also precipitate naturally as inclusion bodies due to various influences during overexpression in the host cell. Although this phenomenon permits easier initial separation from native proteins, these inclusion bodies must carefully be differentially solubilized so as to reform functional, correctly folded …


Survey Results: Complexities And Overlaps In Existing Citizen Science Mosquito Projects, Yujia He, Elizabeth Tyson Jan 2017

Survey Results: Complexities And Overlaps In Existing Citizen Science Mosquito Projects, Yujia He, Elizabeth Tyson

Patterson School of Diplomacy and International Commerce Faculty Publications

Currently, multiple projects worldwide engage the public in the scientific process of learning about mosquito biodiversity or monitoring mosquitoes that carry diseases like Dengue, Chikungunya, Zika and Malaria. These projects are executed at different invasion-stages and different scenarios of epidemiological risk based on their country of origin. Through surveying 12 international research teams, this brief report illuminates the differences and the overlaps in the protocols and tools used by existing citizen science mosquito monitoring projects. The analysis covers project goals, requirements and support for participants, policy impact, digital tools and data privacy.


Conditions Responsible For The Success Of Carnivorous Plants In Nutrient-Poor Wetlands, Matthew John Abbott Jan 2017

Conditions Responsible For The Success Of Carnivorous Plants In Nutrient-Poor Wetlands, Matthew John Abbott

Electronic Theses and Dissertations

Despite the vast array of past research focused on carnivorous plants, few studies have investigated the ecological interactions between carnivorous and non-carnivorous plant species. I addressed the following three questions: (1) does niche complementarity promote coexistence between fire-adapted carnivorous and non-carnivorous species? (2) do carnivorous plants rely on both leaf traps and arbuscular mycorrhizal fungi to access different nutrients that are in high demand after fire in nutrient poor bogs? (3) why are carnivorous plants largely absent from nutrient-rich wetlands? I addressed the first question by examining the three-way interacting effects of fire, prey-derived nutrient availability, and root competition from …


A Kinematic Comparison Of Shoulder And Elbow Dynamics Influenced By The Shoe-Surface Interface In Youth And Adolescent Baseball Pitchers, Jacob Richard Gdovin Jan 2017

A Kinematic Comparison Of Shoulder And Elbow Dynamics Influenced By The Shoe-Surface Interface In Youth And Adolescent Baseball Pitchers, Jacob Richard Gdovin

Electronic Theses and Dissertations

From 1994-2006, 1.5 million baseball injuries were treated within emergency departments across the United States while the upper extremity was the second most commonly injured body part. Although it has been determined that pitch count, pitch type and pitching mechanics are the main contributors to upper extremity pain in baseball pitchers, footwear has not been considered a potential factor. Therefore, the purpose of this study was to determine how baseball-specific footwear [turf shoes (ts) and molded cleats (mc)] affects the shoulder and elbow dynamics of youth and adolescent pitchers during an overhead pitch on various surface inclinations [flat ground (fg) …


Evaluation Of Nutrition Education In The Families In Transformation (Fit) Program., Sydney Anne Devers Jan 2017

Evaluation Of Nutrition Education In The Families In Transformation (Fit) Program., Sydney Anne Devers

Electronic Theses and Dissertations

No abstract provided.


The Impact Of Simulated Microgravity On The Growth Of The Model Legume Plant Medicago Truncatula, Gemma Elizabeth Lionheart Jan 2017

The Impact Of Simulated Microgravity On The Growth Of The Model Legume Plant Medicago Truncatula, Gemma Elizabeth Lionheart

Electronic Theses and Dissertations

Simulated microgravity has been a useful tool to help understand plant development in altered gravity conditions. Thirty-one genotypes of the legume plant Medicago truncatula were grown in either simulated microgravity on a rotating clinostat, or a static, vertical environment. Twenty morphological features were measured and compared between these two gravity treatments. Within-species genotypic variation was a significant predictor of the phenotypic response to gravity treatment in 100% of the measured morphological and growth features. In addition, there was a genotype–environment interaction (G×E) for 45% of the response variables, including shoot relative growth rate (p < 0.0005), median number of roots (p ∼ 0.02), and root dry mass (p < 0.005). These findings are discussed in the context of improving future studies in plants space biology by controlling for genotypic differences, and by connecting traits to their underlying genetic causes by using genome-wide association (GWA) mapping. In the long-term, manipulation of genotype effects, in combination with M. truncatula’s symbiotic relationships with rhizobacteria and arbuscular mycorrhizal fungi, will be important for optimizing legumes for cultivation on long-term space missions.


The Reptile Gut Microbiome: Its Role In Host Evolution And Community Assembly, Timothy John Colston Jan 2017

The Reptile Gut Microbiome: Its Role In Host Evolution And Community Assembly, Timothy John Colston

Electronic Theses and Dissertations

I characterize the endogenous (gut) microbiome of Squamate reptiles, with a particular focus on the suborder Serpentes, and investigate the influence of the microbiome on host evolution and community assembly using samples I collected across three continents in the New and Old World. I developed novel methods for sampling the microbiomes of reptiles and summarized the current literature on non-mammalian gut microbiomes. In addition to establishing a standardized method of collecting and characterizing reptile microbiomes I made novel contributions to the future direction of the burgeoning field of host-associated microbiome research. Through persistent and rigorous fieldwork I amassed the largest …


Risk, Reward, And The Role Of Habitat Selection On Community Dynamics In Temporary Ponds, Matthew Robert Pintar Jan 2017

Risk, Reward, And The Role Of Habitat Selection On Community Dynamics In Temporary Ponds, Matthew Robert Pintar

Electronic Theses and Dissertations

The processes that generate patterns of species diversity within and among habitat patches are a fundamental topic of interest in ecology. Traditionally, there was a focus on post-colonization effects such as predation, however, pre-colonization processes, such as habitat selection, are equally important determinants of community structure. In freshwater systems, the lethal effects of dominant predators (particularly fish) are well established, while the transition from permanent to temporary ponds is a defining characteristic. This transition produces distinct communities characterized by organisms with complex life cycles and plastic developmental strategies. This means that many organisms occupying habitat patches arrive there by the …


Food Insecurity And Eating Behaviors Of Collegiate Male Athletes, Kacie Poll Jan 2017

Food Insecurity And Eating Behaviors Of Collegiate Male Athletes, Kacie Poll

Electronic Theses and Dissertations

PURPOSE: To assess the relationship of high school (HS) and collegiate (OM) household food security status (FSS) to current food consumption behaviors in a sample of NCAA Division 1 male, collegiate athletes ≥18 years of age (y). METHODS: A cross-sectional survey of NCAA athletes from a Southeastern Conference (SEC) university was approved by the Institutional Review Board and conducted during August-September, 2016. The questionnaire included the USDA food security survey module and questions related to disordered eating behaviors and food hoarding USDA methods were used to calculate FSS. RESULTS: Participating athletes (n=111) were 19.7±1.4 y and primarily white, football players …


Impact Of Hydration On Collegiate Athletic Performance, Fatigue, And Recovery, Lauren Woodard Jan 2017

Impact Of Hydration On Collegiate Athletic Performance, Fatigue, And Recovery, Lauren Woodard

Electronic Theses and Dissertations

The impact of dehydration on several key physiological functions that are critical for athletic performance is well studied. Hydration monitoring has been known to have significant value on impaired physical functions by maximizing performance during training and competition. Impaired physical functions has been suggested as a limitation on explosive strength, musculoskeletal output, cardiovascular output, and proper technique in contact sports. The purpose of this study was to investigate the relationship between hydration levels and physiological profiles that can be used as a proactive approach in monitoring a collegiate athlete’s athletic performance, fatigue, and recovery status. It was hypothesized that insufficient …


Relationships Between Dietary Habits, Demographics, And Hike Outcome Among 2015 Pacific Crest Trail Backpackers, Lauren Ann Black Jan 2017

Relationships Between Dietary Habits, Demographics, And Hike Outcome Among 2015 Pacific Crest Trail Backpackers, Lauren Ann Black

Electronic Theses and Dissertations

This quantitative research study explores the relationship between dietary patterns, hiker demographics, and total miles hiked during a backpacking trip on the Pacific Crest Trail (PCT). Long hiking distances, low rate of thru-hike completion, consumption of processed and ready to eat foods, weight loss, and large time and monetary strains are comthemes accepted anecdotally by the backpacking population11. However, these themes have yet to be adequately researched and validated for long distance backpackers and PCT backpackers specifically. The online survey gathered data from 151 backpackers who hiked the PCT in 2015. Significant positive correlational relationships were found between the number …


Effects Of Carbohydrate And Branched-Chain Amino Acid Beverage Ingestion During Acute Upper Body Resistance Exercise On Performance And Postexercise Hormone Response, Johneric W. Smith, Ben M. Krings, Brandon D. Shepard, Hunter S. Waldman, Steven A. Basham, Matthew J. Mcallister Jan 2017

Effects Of Carbohydrate And Branched-Chain Amino Acid Beverage Ingestion During Acute Upper Body Resistance Exercise On Performance And Postexercise Hormone Response, Johneric W. Smith, Ben M. Krings, Brandon D. Shepard, Hunter S. Waldman, Steven A. Basham, Matthew J. Mcallister

Kinesiology

The purpose of this investigation was to examine the individual and combined effects of ingesting carbohydrates (CHO) and branched-chain amino acids (BCAA) during high-volume upper body resistance exercise (RE) on markers of catabolism and performance. Thirteen resistance-trained males completed 4 experimental trials with supplementation, ingesting beverages containing CHO, BCAA, CHO plus BCAA, or placebo (PLA) in a randomized, double-blind design.


Biochemical And Molecular Assessment Of Toxicity Of Primaquine Metabolites On Erythrocytes, Jagrati Jain Jan 2017

Biochemical And Molecular Assessment Of Toxicity Of Primaquine Metabolites On Erythrocytes, Jagrati Jain

Electronic Theses and Dissertations

The 8-aminoquinoline (8AQ) antimalarial drug primaquine (PQ) is the only drug for prevention of malaria relapse. Moreover, PQ also has gametocytocidal activity against Plasmodium falciparum. However, clinical use of PQ has been limited due to its hemolytic toxicity, especially in glucose-6-phosphate dehydrogenase deficient (G6PDd) individuals. Phenolic and quinone metabolites generated via cytochrome P450-dependent pathways appear to be responsible for hemolytic effects of PQ. However, the mechanism for the hemolytic toxicity of PQ is still poorly understood. To explore the mechanism, targets, and pathways for toxicity of PQ, normal and G6PDd human erythrocytes were treated with the potential hemotoxic metabolites of …


Nucleic Acid Clamp-Mediated Transcriptional And Translational Modulation Of Oncogenes Via The Stabilization Of G-Quadruplexes, Taisen Hao Jan 2017

Nucleic Acid Clamp-Mediated Transcriptional And Translational Modulation Of Oncogenes Via The Stabilization Of G-Quadruplexes, Taisen Hao

Electronic Theses and Dissertations

G-quadruplexes (G4s) are non-canonical secondary nucleic acid structures that exit in various biologically important regions within a cell, such as the promoters of DNA, 5’-Untranslated regions (UTR) of mRNA and telomeres. These secondary structures tend to cluster around 1 kb from transcriptional start sites across all human chromosomes in addition to at telomeric regions. Extensive efforts from many research groups have been dedicated to targeting G4s in oncogenic promoters, telomeres, and 5’-UTRs with traditional small molecules. However, most small molecules recognizing multiple G4s raising potential issues in terms of utilizing these small molecules clinically. Therefore, we developed a novel nucleic …


Historical Refuges And Recolonization Routes In The Southern Appalachian Mountains, Inferred Through Phylogeographic Analysis Of The Spotted Wintergreen (Chimaphila Maculata), John Daniel Banusiewicz Jan 2017

Historical Refuges And Recolonization Routes In The Southern Appalachian Mountains, Inferred Through Phylogeographic Analysis Of The Spotted Wintergreen (Chimaphila Maculata), John Daniel Banusiewicz

Electronic Theses and Dissertations

Phylogeography has recently benefited from incorporation of coalescent modeling to test competing scenarios of population history of a species. Ecological niche modeling has also been useful in inferring areas of likely suitable habitat during past climate conditions. Several studies have examined the population history of biota in the Southern Appalachian Mountains and how they responded to climate change associated with the end of the Last Glacial Maximum (~18,000 years ago), though few studies have focused on understory plants. This study redressed that knowledge gap thorough examination of the phylogeographic history of the understory plant Chimaphila maculata, which is native to …


An Evaluation Of The Mississippi Recipes For Success Resource From The Perspective Of Child Nutrition Directors, Chelsea L. Bell Jan 2017

An Evaluation Of The Mississippi Recipes For Success Resource From The Perspective Of Child Nutrition Directors, Chelsea L. Bell

Electronic Theses and Dissertations

The Mississippi Recipes for Success (MRS), A Guide for Child Nutrition Programs provides printed and online resources for child nutrition directors. These resources include recipes and methods to assist directors in effectively implementing United States Department of Agriculture (USDA) nutrition standards. The purpose of this study was to investigate if child nutrition directors utilize these resources, as well as assess child nutrition directors’ perceptions of the helpfulness, satisfaction, and importance of the various components of the online and 2014 printed versions of the MRS. Data collection for this study involved an online survey that evaluated directors’ usage of the resources …


Contribution Of Specific Amino Acids To Calcium-Dependent Dimerization Of Epithelial Cadherin, Xiaoyun Z. Howard Jan 2017

Contribution Of Specific Amino Acids To Calcium-Dependent Dimerization Of Epithelial Cadherin, Xiaoyun Z. Howard

Electronic Theses and Dissertations

Cadherins are calcium-dependent molecules that play essential roles in embryonic development, tissue morphogenesis, and cancer progression. Epithelial (E-) cadherin is present at adherens junctions, structures that are important for maintaining tissue integrity. At adherens junctions, cell–cell adhesion complexes are formed as clusters of cadherin dimers through strand-swapped dimerization between two interacting protomers from adherent cells. Recently, a number of studies of dimer formation of E-cadherin supported a two-step adhesive binding model. First, an inter-molecular initial encounter complex (X-dimer) forms at the linker region of the first two extracellular domains, EC1 and EC2. Next, strand-swap dimerization occurs via exchange or ''swap'' …


Bioelectrohydrogenesis And Inhibition Of Methanogenic Activity In Microbial Electrolysis Cells - A Review, Rengasamy Karthikeyan, Ka Yu Cheng, Ammaiyappan Selvam, Arpita Bose, Jonathan W.C. Wong Jan 2017

Bioelectrohydrogenesis And Inhibition Of Methanogenic Activity In Microbial Electrolysis Cells - A Review, Rengasamy Karthikeyan, Ka Yu Cheng, Ammaiyappan Selvam, Arpita Bose, Jonathan W.C. Wong

Biology Faculty Research

Microbial electrolysis cells (MECs) are a promising technology for biological hydrogen production. Compared to abiotic water electrolysis, a much lower electrical voltage (~0.2V) is required for hydrogen production in MECs. It is also an attractive waste treatment technology as a variety of biodegradable substances can be used as the process feedstock. Underpinning this technology is a recently discovered bioelectrochemical pathway known as "bioelectrohydrogenesis". However, little is known about the mechanism of this pathway, and numerous hurdles are yet to be addressed to maximize hydrogen yield and purity. Here, we review various aspects including reactor configurations, microorganisms, substrates, electrode materials, and …


Species Distribution Model Of Invasive Alien Species Acacia Nilotica For Central-Eastern Indonesia Using Biodiversity Climate Change Virtual Laboratory (Bccvl), Sutomo, Eddie Van Etten Jan 2017

Species Distribution Model Of Invasive Alien Species Acacia Nilotica For Central-Eastern Indonesia Using Biodiversity Climate Change Virtual Laboratory (Bccvl), Sutomo, Eddie Van Etten

Research outputs 2014 to 2021

Climate change may facilitate alien species invasion into new areas. This study uses Biodiversity and Climate Change Virtual Laboratory to develop a species distribution model (SDM) of Acacia nilotica (L.) Willd. ex Delile. based upon its naturalized distribution to project the potential distribution of A. nilotica throughout tropical environment of Indonesia under current and future climate conditions. Global biodiversity information facility database was utilized to obtain the species occurrences data. The climate factors were precipitation and temperature layers, available in Worldclim current conditions (1950-2000) at 2.5 arcmin. We used Generalized Linear Model. The result was then projected to the year …


Examination Of Civil Forensic Entomology Through Case Studies, Alicia Thuis Jan 2017

Examination Of Civil Forensic Entomology Through Case Studies, Alicia Thuis

Department of Entomology: Distance Master of Science Projects

Brief History of Forensic Entomology 2 Civil Forensic Case Studies 11 The Raisin Ant 11 Maggots in My Potato! 13 Extra Protein for Fido 15 Health Food Invasion 16 Swimming Spiders 18 Extras in the Steak 19 In or Out? 23 Buttery Parasites 24 Laminated Flies 26 Chicken Fried Maggots 27 Process of Elimination 29 Bed Bugs Everywhere! 30 Not a “Bug” 32 Not a Cockroach 34 Ethical Considerations 36 Best Practice Recommendations 43 Future Research Directions 47 References 50


Biology And Management Of Key Rice Pest In Asia, Airene Millsap Jan 2017

Biology And Management Of Key Rice Pest In Asia, Airene Millsap

Department of Entomology: Distance Master of Science Projects

1. Importance of rice

2. Origin and history of rice as a crop

3. Rice cultivation 3.1 Lowland or Irrigated rice 3.2 Upland rice 3.3 Deep water rice 3.4 Floating rice

4. Parts of rice plants 4.1 Vegetative stage 4.2 Reproductive stage 4.3 Ripening stage

5. Rice Pests

5.1 Vegetative stage • Stem Borer • Gall Midge • Rice Hispa • Rice Leaf Folder • Rice Armyworms • Rice Whorl Maggots • Rice Caseworm • Rice Mealybugs • Short Horned Grasshopper • Rice Stem Maggot • Rice Thrips • Oriental Migratory Locust • Black Bug • Green Stink Bug

5.2 …


Entomophagy: Insect And Arthropod Grub!, Buddy Roper Jan 2017

Entomophagy: Insect And Arthropod Grub!, Buddy Roper

Department of Entomology: Distance Master of Science Projects

Recipes:

Appetizers………………………………. 3

Entrees………………………………….. 27

Desserts…………………………………. 44

Recipe Sources…………………………. 56

Discussion and PowerPoint slides attached below as supplemental file


Interdependent Relationships And Biodiversity, Lena Teselle Jan 2017

Interdependent Relationships And Biodiversity, Lena Teselle

Department of Entomology: Distance Master of Science Projects

This project is a composite of three Next Generation Science Standard(NGSS) aligned lessons aimed at middle school science students. Three E-Learning modules have been developed and uploaded to www.DiscoverEntomology.comto supplement the classroom lessons in a flipped model, whereby students complete the online lesson prior to attending class, application of concepts are explored through activity. Over the course of five to six class sessions and a field trip, students will build their understanding of interactions within ecosystems, the interdependent relationships that form between and among organisms, and the importance of biodiversity, using insects as model study subjects. This project serves to …


The Red Imported Fire Ant: The Visitor Who Wouldn’T Leave. A Brief Overview Of The Control Of Solenopsis Invicta In The United States, Melissa Brown Jan 2017

The Red Imported Fire Ant: The Visitor Who Wouldn’T Leave. A Brief Overview Of The Control Of Solenopsis Invicta In The United States, Melissa Brown

Department of Entomology: Distance Master of Science Projects

The ease at which people, plants and animals move across the globe has created the perfect vehicle for transporting pests and disease. The introduction of non-native species into an ecosystem is a cause for great concern. The economic impact alone makes this a situation that effects everyone. The Red Imported Fire Ant, Solenopsis invicta Buren has become a major agricultural and urban pest throughout the southeastern United States. In addition, fire ants cause both medical and environmental harm (Stimac and Alves 1994). The cost associated with the control of the RIFA is significant. An estimated cost of about $36 per …


Rag1 Soybean Photosynthetic Response To Biotype 2 Aphid Colonization And Feeding, Katy Hillard Jan 2017

Rag1 Soybean Photosynthetic Response To Biotype 2 Aphid Colonization And Feeding, Katy Hillard

Department of Entomology: Distance Master of Science Projects

Over the past ten years, the soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), has been one of the principal insect threats to commercial soybean, Glycine max (L.) Merr., production in the United States. Resistance genes identified from native soybean have been incorporated into commercial varieties to provide protection against aphid feeding damage. The biology of the pest lends itself to rapid buildup of biotypes capable of overcoming host plant resistance. Single gene resistance sources have displayed low levels of durability against aphids. The first aphid resistant commercial soybean line released in the United States contained the Rag1 gene, which was …