Life Sciences Commons^™

Redd Habitat Selection For Brook Trout In The Necktie River Bemidji, Mn, William Varela, Zachary Buckley

Journal of Earth and Life Science

The presence of quality suitable habitat is important for the success of naturally reproducing Brook Trout populations. Therefore, measuring redd habitat Brook Trout select for was the focus of this study. Brook Trout redds were observed during the spawning season of 2016. We identified a total of 18 redds in the Necktie River in late October and early November. Specific environmental variables associated with redd selection were measured: velocity, in-stream cover, canopy cover, bank overhang, river width, substrate type and size, temperature, and max-depth. The same environmental variables were measured for 30 randomly selected sites. Redds were distributed in riffles …

Influence Of Time Spent In Captivity On Aggressive Behaviors Of Gray Wolves Canis Lupus, Chelsea Knutson

Journal of Earth and Life Science

Knowledge of the characteristics that promote group success is key to understanding the adaptive advantages of sociality for many group-oriented species. In this study, nine Gray Wolves Canis lupis were each observed twelve times over a period of six months to determine if the amount of time spent in the wild/captivity had any effect on aggressive behaviors. The number of aggressive behaviors were recorded for each wolf during each observation. The nine wolves were separated into three groups depending on their upbringing; born and raised in captivity, born in the wild and raised in captivity, and born and raised in …

144 Effect Of Antral Follicle Count In Beef Heifers On In Vitro Fertilization/Production, C. C. Chase Jr., R. A. Freeland, A. K. Mcneel, E. C. Wright-Johnson, G. A. Perry, S. C. Tenley, J. R. Wood, A. S. Cupp, J. L. Vallet, J. R. Miles

Department of Animal Science: Faculty Publications

Our objective has been to compare the IVF and in vitro production (IVP) of embryos from low and high antral follicle count (AFC) heifers. This is the fourth year of the study with years 1 to 3 reported individually. For this report, we add data for the fourth year and present a combined analysis (years 1 to 4) for the first time. Each year, AFC was determined on ~120 Angus heifers using transrectal ultrasonography. Ten heifers with the lowest AFC and 10 heifers with the highest AFC and all with evidence of oestrous cyclicity were synchronized with two 5-mL injections …

Medical Subject Heading (Mesh) Annotations Illuminate Maize Genetics And Evolution, Timothy M. Beissinger, Gota Morota

Department of Animal Science: Faculty Publications

Background: High-density marker panels and/or whole-genome sequencing, coupled with advanced phenotyping pipelines and sophisticated statistical methods, have dramatically increased our ability to generate lists of candidate genes or regions that are putatively associated with phenotypes or processes of interest. However, the speed with which we can validate genes, or even make reasonable biological interpretations about the principles underlying them, has not kept pace. A promising approach that runs parallel to explicitly validating individual genes is analyzing a set of genes together and assessing the biological similarities among them. This is often achieved via gene ontology analysis, a powerful tool that …

Production Of A Gonadotropin-Releasing Hormone 2 Receptor Knockdown (Gnrhr2 Kd) Swine Line, A. T. Desaulniers, R. A. Hou, G. A. Mills, C. A. Lents, Brett R. White

Department of Animal Science: Faculty Publications

Swine are the only livestock species that produce both the second mammalian isoform of gonadotropin-releasing hormone (GNRH2) and its receptor (GNRHR2). Previously, we reported that GNRH2 and GNRHR2 mediate LH-independent testosterone secretion from porcine testes. To further explore this ligand-receptor complex, a pig model with reduced GNRHR2 expression was developed. Small hairpin RNA sequences targeting porcine GNRHR2 were subcloned into a lentiviral-based vector, lentiviral particles were generated and microinjected into the perivitelline space of zygotes, and embryos were transferred into a recipient. One GNRHR2 knockdown (KD) female was born that subsequently produced 80 piglets from 6 litters with 46 hemizygous …

Physically Adjusted Neutral Detergent Fiber System For Lactating Dairy Cow Rations. I: Deriving Equations That Identify Factors That Influence Effectiveness Of Fiber, Robin R. White, Mary Beth Hou, Jeffrey L. Firkins, Paul J. Kononoff

Department of Animal Science: Faculty Publications

Physically effective neutral detergent fiber (peNDF) is the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen. Multiplying dietary NDF by particle size has been used as an estimate of peNDF. In re-evaluating the concept of peNDF, we compared the use of peNDF as dietary NDF × particle size with the use of individual NDF and particle size descriptors (physically adjusted NDF; paNDF) when used with other physical and chemical diet descriptors to predict dry matter (DM) intake (DMI), rumination time, and ruminal pH in lactating dairy …

Expression Of Cytokine Genes And Receptors In White Blood Cells Associated With Divergent Body Weight Gain In Beef Steers, Amanda K. Lindholm-Perry, Virginia M. Cederberg, Jeremy R. Miles, Andrew P. Foote

Department of Animal Science: Faculty Publications

Previous work examining the transcriptome of steer tissue samples from animals with divergent gain have shown a relationship with the expression of genes with functions in immune and inflammatory pathways. The process of mounting an immune or inflammatory response is energetically expensive and variation in cytokine responses may affect cattle production traits. In addition, a previous study has identified variation in the transcript abundance of numerous genes, including the cytokine gene IL6ST, in the circulating white blood cells of pigs associated with high and low residual feed intake (RFI) lines. The aim of this study was to determine whether …

Economic Selection Index Development For Beefmaster Cattle I: Terminal Breeding Objective, Kathleen P. Ochsner, M. D. Macneil, Matthew L. Spangler

Department of Animal Science: Faculty Publications

The objective of this study was to develop an economic selection index for Beefmaster cattle in a terminal production system where bulls are mated to mature cows with all resulting progeny harvested. National average prices from 2010 to 2014 were used to establish income and expenses for the system. Phenotypic and genetic parameter values among the selection criteria and goal traits were obtained from literature. Economic values were estimated by simulating 100,000 animals and approximating the partial derivatives of the profit function by perturbing traits one at a time, by 1 unit, while holding the other traits constant at their …

The Impact Of Training Strategies On The Accuracy Of Genomic Predictors In United States Red Angus Cattle, J. Lee, Stephen D. Kachman, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Genomic selection (GS) has become an integral part of genetic evaluation methodology and has been applied to all major livestock species, including beef and dairy cattle, pigs, and chickens. Significant contributions in increased accuracy of selection decisions have been clearly illustrated in dairy cattle after practical application of GS. In the majority of U.S. beef cattle breeds, similar efforts have also been made to increase the accuracy of genetic merit estimates through the inclusion of genomic information into routine genetic evaluations using a variety of methods. However, prediction accuracies can vary relative to panel density, the number of folds used …

Genomics Symposium: Using Genomic Approaches To Uncover Sources Of Variation In Age At Puberty And Reproductive Longevity In Sows, Hiruni R. Wijesena, Clay A. Lents, Jean-Jack Riethoven, Melanie D. Trenhaile-Grannemann, Jennifer F. Thorson, Brittney N. Keel, Phillip S. Miller, Matt Spangler, Stephen D. Kachman, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Genetic variants associated with traits such as age at puberty and litter size could provide insight into the underlying genetic sources of variation impacting sow reproductive longevity and productivity. Genomewide characterization and gene expression profiling were used using gilts from the University of Nebraska–Lincoln swine resource population (n = 1,644) to identify genetic variants associated with age at puberty and litter size traits. From all reproductive traits studied, the largest fraction of phenotypic variation explained by the Porcine SNP60 BeadArray was for age at puberty (27.3%). In an evaluation data set, the predictive ability of all SNP from highranked 1-Mb …

The Roles Of Age At Puberty And Energy Restriction |In Sow Reproductive Longevity: A Genomic Perspective, Hiruni R. Wijesena, Clay A. Lents, Melanie D. Trenhaile-Grannemann, Jean-Jack Riethoven, Brittney N. Keel, Jennifer F. Thorson, Phillip S. Miller, Rodger K. Johnson, Matthew L. Spangler, Stephen D. Kachman, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Approximately 50% of sows are culled annually with more than one-third due to poor fertility. Our research demonstrated that age at puberty is an early pre-breeding indicator of reproductive longevity. Age at puberty can be measured early in life, has a moderate heritability, and is negatively correlated with lifetime number of parities. Detection of age at puberty is tedious and time consuming and is therefore not collected by the industry, which limits genetic progress. Genomic prediction is a viable approach to preselect gilts that will express puberty early and have superior reproductive longevity. The hypothesis that genetic variants explaining differences …

Economic Selection Index Development For Beefmaster Cattle Ii: General-Purpose Breeding Objective, Kathleen P. Ochsner, M. D. Macneil, Ronald M. Lewis, Matthew L. Spangler

Department of Animal Science: Faculty Publications

An economic selection index was developed for Beefmaster cattle in a general-purpose production system in which bulls are mated to a combination of heifers and mature cows, with resulting progeny retained as replacements or sold at weaning. National average prices from 2010 to 2014 were used to establish income and expenses for the system. Genetic parameters were obtained from the literature. Economic values were estimated by simulating 100,000 animals and approximating the partial derivatives of the profit function by perturbing traits 1 at a time, by 1 unit, while holding the other traits constant at their respective means. Relative economic …

Development Of An Objective Feet And Leg Conformation Evaluation Method Using Digital Imagery In Swine, J. D. Stock, J. A. Calderon Diaz, C. E. Abell, T. J. Baas, M. F. Rothschild, B. E. Mote, K. J. Stalder

Department of Animal Science: Faculty Publications

Background:The objectives of this study were to create an objective measurement method of joint angles for knee, hock, front and rear pasterns and a rear stance position in swine using digital imaging technology and to assess the repeatability of the objective measurement process. Methods and Findings: Forty-five multiparous sows (average parity 6.7 ± 2.5; parity range 5 to 14) from two commercial farms (n=21 farm 1 and n=24 farm 2) were used. Sows were moved to a pen where digital images of the profile and rear stance were captured. On average, 5.2 (± 2.6) profile and 2.6 (± 1.0) …

Effect Of Partially Replacing A Barley-Based Concentrate With Flaxseed-Based Products On The Rumen Bacterial Population Of Lactating Holstein Dairy Cows, E. Castillo-Lopez, J. Moats, N. D. Aluthge, H. A. Ramirez Ramirez, D. A. Christensen, T. Mutsvangwa, G. B. Penner, S. C. Fernando

Department of Animal Science: Faculty Publications

Aims: The effects of partial replacement of a barley-based concentrate with flaxseed-based products on the rumen bacterial population of lactating Holstein dairy cows were evaluated. Methods and Results: Treatments fed were CONT, a normal diet that included barley silage, alfalfa hay and a barley-based concentrate that contained no flaxseed or faba beans; FLAX, inclusion of a nonextruded flaxseed-based product containing 55-0% flaxseed, 37-8% field peas and 6-9% alfalfa; EXT, similar to FLAX, but the product was extruded and EXTT, similar to FLAX, but product was extruded and field peas were replaced by high-tannin faba beans. The rumen bacterial population was …

Genomic Relatedness Strengthens Genetic Connectedness Across Management Units, Haipeng Yu, Matthew L. Spangler, Ronald M. Lewis, Gota Morota

Department of Animal Science: Faculty Publications

Genetic connectedness refers to a measure of genetic relatedness across management units (e.g., herds and flocks). With the presence of high genetic connectedness in management units, best linear unbiased prediction (BLUP) is known to provide reliable comparisons between estimated genetic values. Genetic connectedness has been studied for pedigree-based BLUP; however, relatively little attention has been paid to using genomic information to measure connectedness. In this study, we assessed genomebased connectedness across management units by applying prediction error variance of difference (PEVD), coefficient of determination (CD), and prediction error correlation r to a combination of computer simulation and real data (mice …

National Beef Quality Audit-2016: Transportation, Mobility, And Harvest-Floor Assessments Of Targeted Characteristics That Affect Quality And Value Of Cattle, Carcasses, And By-Products, L.C. Eastwood, C A. Boykin, M.K. Harris, A.N. Arnold, D.S. Hale, C R. Kerth, D.B. Griffin, J.W. Savell, K.E. Belk, D.R. Woerner, J.D. Hasty, R.J. Delmore Jr., J.N. Martin, T.E. Lawrence, T.J. Mcevers, D.L. Vanoverbeke, G.G. Mafi, M.M. Pfeiffer, Ty B. Schmidt, R.J. Maddock, D.D. Johnson, C.C. Carr, J. M. Scheffler, T.D. Pringle, A.M. Stelzleni

Department of Animal Science: Faculty Publications

The National Beef Quality Audit-2016 (NBQA-2016) was conducted to assess current transportation, mobility, and quality characteristics of U.S. fed steers and heifers. Data were collected at 17 beef processing facilities between March and November 2016. About 8,000 live cattle were evaluated for transportation and mobility, and about 25,000 carcasses were evaluated on the slaughter floor. Cattle were in transit to the slaughter facility for a mean duration of 2.7 h from a mean distance of 218.5 km using trailers with dimensions ranging from 17.84 m2 to 59.09 m2. Area allotted per animal averaged 1.13 m2 and ranged from 0.85 m2 …

National Beef Quality Audit–2016: Transportation, Mobility, Live Cattle, And Carcass Assessments Of Targeted Producer-Related Characteristics That Affect Value Of Market Cows And Bulls, Their Carcasses, And Associated By-Products, M.K. Harris, L.C. Eastwood, C.A. Boykin, A.N. Arnold, K.B. Gehring, D.S. Hale, C.R. Kerth, D.B. Griffin, J.W. Savell, K.E. Belk, D.R. Woerner, J.D. Hasty, R.J. Delmore Jr., J.N. Martin, T.E. Lawrence, T.J. Mcevers, D.L. Vanoverbeke, G.G. Mafi, M.M. Pfeiffer, Ty B. Schmidt, R.J. Maddock, D.D. Johnson, C.C. Carr, J.M. Scheffler, T.D. Pringle, A.M. Stelzleni

Department of Animal Science: Faculty Publications

The National Beef Quality Audit–2016 marks the fourth iteration in a series assessing the quality of live beef and dairy cows and bulls and their carcass counterparts. The objective was to determine the incidence of producer-related defects, and report cattle and carcass traits associated with producer management. Conducted from March through December of 2016, trailers (n = 154), live animals (n = 5,470), hide-on carcasses (n = 5,278), and hide-off hot carcasses (n = 5,510) were surveyed in 18 commercial packing facilities throughout the United States. Cattle were allowed 2.3 m2 of trailer space on average during transit indicating some …

A Potential Role For Mtorc1/2 In Β2 Adrenergic Regulation Of Skeletal Muscle Glucose Oxidation In Models Of Intrauterine Growth Restriction, Robert J. Posont, Caitlin N. Cadaret, Taylor L. Barnes, Dustin T. Yates

Department of Animal Science: Faculty Publications

The epidemic of intrauterine growth restriction (IUGR) continues to be a leading cause of perinatal morbidity and mortality throughout the world. This condition has been linked to the development of metabolic health problems such as obesity, hypertension, glucose intolerance, and type 2 diabetes at all ages. Previous studies have demonstrated that IUGR fetal adaptations impair proper glucose homeostasis in part via changes in insulin responsiveness in key tissues including skeletal muscle and liver, and that these deficits persists into adulthood. Many components of insulin signaling pathways associated with glucose metabolic regulation have been evaluated in IUGR tissues for adaptive changes. …

Political Ecology Of Medicinal Plant Use In Rural Nepal: Globalization, Environmental Degradation, And Cultural Transformation, Emily Dovydaitis

Honors Undergraduate Theses

Prior to the advent of biomedicine, rural communities in Nepal relied on phytochemically active compounds in medicinal plants as their primary source of medicine; however, ethnobotanical practices have shifted over time due to economic, environmental, and sociocultural stimuli. Findings from 2016 fieldwork conducted in Dumrikharka, Nepal and Tutung, Nepal are compared to existing literature to describe the political ecology of medicinal plants in rural Nepal.

Anthropogenic climate change threatens individual plant species and ecosystem biodiversity. Globalized markets unabated by weak conservation programs place increasing demands on medicinal plants. As indigenous plants become overharvested and more difficult to access, Nepalis incorporate …

The Role Of The Y-Chromosome In The Evolution Of Autosomally Coded Traits, Ian Kutch

Electronic Theses and Dissertations

Recent work indicates that the Y-chromosome of the fruit fly Drosophila melanogaster can influence gene regulation on the autosomes and X chromosome. This newly discovered function of the Y has the potential to dramatically shape the regulatory evolution of numerous genes that reside throughout the genome; even for genes that code for both male and female traits. Given that the mechanism underlying the Y-linked influence on gene expression in D. melanogaster appears to exist in other independently evolved heterogametic sex chromosomes, the evolutionary implications of Y-linked regulatory variation (YRV) deserves to be explored. These implications include the potential for Y-chromosomes …

Journal Of The Arkansas Academy Of Science- Volume 71 2017, Academy Editors

Journal of the Arkansas Academy of Science

No abstract provided.

The Fleas (Arthropoda: Insecta: Siphonaptera) Of Arkansas, Chris T. Mcallister, Lance A. Durden, Henry W. Robison, Matthew B. Connior

Journal of the Arkansas Academy of Science

Fleas (Insecta: Siphonaptera) are important ectoparasites of cats, dogs, other mammals (including humans), and birds, and are an important component of the biota of North America. In addition, they can be nuisance biters and serve as vectors or intermediate hosts of several flea-borne disease agents and parasites that negatively affect mammals and birds. In Arkansas, there have been no recent comprehensive summaries of fleas in the last 45+ years. Here, we provide a summary of the 29 species of fleas within 7 families that have been recorded from the state, update their taxonomy, and note their medical and veterinary importance.

Local Scale Comparisons Of Avian And Woody Vegetation Communities Within Four Arkansas State Parks, Bennett P. Grooms, Rachael E. Urbanek

Journal of the Arkansas Academy of Science

Measuring the spatial distribution of biotic communities can provide useful data to wildlife managers on how and why species assemblages differ across a landscape. During 18 May – 7 August 2015, we conducted avian point counts and collected vegetation data in nested subplots at 4 Arkansas state parks. We then used a series of one-way ANOVAs and Kruskal-Wallis tests to examine differences in species richness, Simpson’s evenness, Simpson’s diversity, and Bray-Curtis similarity across the 4 parks. Mount Magazine State Park had the lowest avian evenness (F_3,22 = 9.57 P = 0.003) and diversity (F_3,22 = 17.8 …

Phylogeography And Vicariant Separation Of Two River Darters, Percina Uranidea And Percina Vigil, From Rivers That Drain The North American Interior Highlands, T. Yamashita, J. M. Rigsby, J. Stoeckel

Journal of the Arkansas Academy of Science

The phylogeography and separation of two river darters, Percina uranidea and P. vigil were investigated through sequencing of the mitochondrial Cytochrome B and Cytochrome Oxidase genes. These molecular markers revealed the darters exhibit moderate genetic divergence between two large river drainage systems within the Mississippi River basin associated with the Interior Highlands of midwestern North America. An additional haplotype network analysis also supported these trends. Phylogenetic divergence dating indicated that population separation between the river systems occurred after recent Pleistocene glacial events rather than an early Pleistocene separation.

Geographic Distribution Records Of Macracanthorhynchus Ingens (Archiacanthocephala: Oligacanthorhynchidae) From The Raccoon, Procyon Lotor In North America, Dennis J. Richardson, A. Leveille, A. V. Belsare, H. S. Al-Warid, M. E. Gompper

Journal of the Arkansas Academy of Science

No abstract provided.

Central Nests Are Heavier And Have Larger Clutches Than Peripheral Nests In Cliff Swallow (Petrochelidon Pyrrohonota) Colonies, S. Osborne, D. R. Leasure, S. Huang, Ragupathy Kannan

Journal of the Arkansas Academy of Science

No abstract provided.

Anatomical Distribution Of Clinostomum Metacercariae In The Tissues Of Pond-Raised Channel Catfish (Ictaluris Puctatus), J. Singleton, James J. Daly Sr., K. Wagner

Journal of the Arkansas Academy of Science

No abstract provided.

Evaluation Of Filtration And Dna Extraction Methods For Environmental Dna Biodiversity Assessments Across Multiple Trophic Levels, Anni Djurhuus, Jesse Port, Collin Closek, Kevan Yamahara, Ofelia Romero-Maraccini, Kristine Walz, Dawn Goldsmith, Reiko Michisaki, Mya Breitbart, Alexandria Boehm, Francisco Chavez

Marine Science Faculty Publications

Metabarcoding of marine environmental DNA (eDNA), originating from tissue, cells, or extracellular DNA, offers the opportunity to survey the biological composition of communities across multiple trophic levels from a non-invasive seawater sample. Here we compare results of eDNA metabarcoding of multiple trophic levels from individual seawater samples collected from a kelp forest in Monterey Bay, California in order to establish methods for future cross-trophic level eDNA analysis. Triplicate 1 L water samples were filtered using five different 47 mm diameter membrane filters (PVDF, PES, GFF, PCTE, and NC) and DNA was extracted from triplicates of each filter-type using three widely-used …

Spatial Variability Of Spanish Sardine (Sardinella Aurita) Abundance As Related To The Upwelling Cycle Off The Southeastern Caribbean Sea, Digna Rueda-Roa, Jeremy Mendoza, Frank E. Muller-Karger, Juan José Cárdenas, Alina Achury, Yrene Astor

Marine Science Faculty Publications

The Sardinella aurita fishery off northeastern Venezuela, region of seasonal wind-driven coastal-upwelling, accounts for 90% of the Caribbean Sea small pelagic catch. This law-protected artisanal fishery takes place up to ∼10 km offshore. The spatial distribution, number of schools, and biomass of S. aurita were studied using eight hydro-acoustic surveys (1995-1998). The study included the analysis of satellite-derived sea surface temperature and chlorophyll-a. Surveys were grouped by strong, weak, and transitional upwelling seasons. Relationships between these observations were analyzed using Generalized Additive Models. Results show that during the primary upwelling season (January-May) sardines were widely distributed in upwelling plumes that …

Remote Estimation Of Biomass Of Ulva Prolifera Macroalgae In The Yellow Sea, Lianbo Hu, Chuanmin Hu, He Ming-Xia

Marine Science Faculty Publications

Since 2008, macroalgal blooms of Ulva prolifera (also called green tides) occurred every summer in the Yellow Sea (YS), causing environmental and economic problems. A number of studies have used satellite observations to estimate the severity of the blooms through estimating the bloom size and duration. However, a critical bloom parameter, namely biomass, has never been objectively determined due to lack of measurements. In this study, laboratory experiments were conducted to measure U. prolifera biomass (wet weight) per unit area and the corresponding spectral reflectance, through which a robust relationship has been established to link biomass per area to …