Life Sciences Commons^™

The Influence Of Diet And Oxidation On Calcium Retention Of The Mitochondria In Fresh Beef, Emery K. Kunze, Nicolas J. Herrera, Katherine I. Domenech-Perez Perez, Michael D. Chao, Chris R. Calkins

Nebraska Beef Cattle Reports

Feeding dried distillers grains (DG) may influence calcium flux postmortem by disrupting the stability of the sarcoplasmic reticulum (SR) membrane, thus leading to a higher post- rigor calcium leakage, resulting in greater activation of calpains and improved tenderness. Mitochondria provide the opportunity to study calcium flux in a controlled, tightly defined environment as a model system for the SR. Cattle were finished on diets containing either 0% DG or 50% DG. Feeding DG increased proportions of PUFA in the SR and mitochondrial membrane. Oxidized mitochondria retained less calcium than non- oxidized mitochondria. Mitochondria from cattle finished on corn tended to …

Comparison Of Diets Collected From Esophageally Fistulated Cows To Forage Quality Estimated From Fecal Analysis, Bethany M. Johnston, Jay D. Jenkins, Jacqueline A. Musgrave Musgrave, L. A. Stalker, Mitchell B. Stephenson

Nebraska Beef Cattle Reports

Differences in forage quality (crude protein and energy) were analyzed between esophageally fistulated diets, analysis of fecal samples with Nutrition Balance Analyzer (NUTBAL) analysis, and analysis of handclipped forage samples. On upland range sites, hand- clipped samples provided forage quality estimates that were closer to esophageally fistulated diets than samples analyzed with the NUTBAL analysis. Aft er one year of data collection, it appears that there may be some inconstancies with the NUTBAL analysis for estimates on rangeland forage quality in the Nebraska Sandhills. More data is needed to verify these results; however, making management supplementations decisions solely on the …

Summer Cocktail Forage Research In The Panhandle Of Nebraska, Karla H. Jenkins, Cody Creech, Robert Higgins Higgins, Josh Buttle

Nebraska Beef Cattle Reports

Four annual forage mixtures containing brown mid- rib sorghum sudangrass or German foxtail millet, cowpeas or soybeans, and forage collards were compared to the sorghum sudangrass or millet as monocultures in the Nebraska High Plains on dryland acres to determine the tonnage, crude protein, and digestibility available for beef cattle. Th e forage mixtures and the millet resulted in greater crude protein than the sorghum sudangrass. Total digestible nutrients of the mixers were similar. Sorghum sudangrass resulted in the most tonnage. These forage options could have been hayed or windrow grazed in the fall and would have likely resulted in …

Impact Of Inoculum Source For In Vitro And In Situ Digestion Procedures Performed On Corn Residue And Grass Samples, Hannah C. Hamilton, Jana L. Harding, Jim C. Macdonald Donald, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

A study was conducted to assess the effects of inoculum source at time of incubation on neutral detergent fiber digestibility, dry matter digestibility, and organic matter digestibility of corn residue samples. Digestibility of neutral detergent fiber was greater for both grass and corn residue when inoculum source came from steers consuming a high corn residue diet. Digestibility of dry matter and organic matter were not different between grass and corn residue. It is not necessary to maintain two sets of donors for in vitro or in situ procedures involving corn residue. However, donor diet affects neutral detergent fiber digestibility estimates …

Evaluation Of Plant- Waxes To Estimate Forage Intake In Grazing Cattle, Hannah C. Hamilton, Jerry D. Volesky, Robert M. Lewis Lewis

Nebraska Beef Cattle Reports

Although key to the efficiency of a cattle operation, feed intake is challenging to evaluate in a grazing setting. However, even within forage- based systems, plant- wax markers may be used to predict dietary choices and feed intake. Plant- waxes are a complex mixture of lipids found on the surface of plants. When sufficiently unique among plants, the composition of diets can be determined from the pattern of these compounds in the forages ingested. These markers were used to delineate the parts of the corn plant and, separately, 8 western rangeland grasses and legumes. Using plant waxes, the components of …

Evaluating The Impacts Of Field Peas In Growing And Finishing Diets On Performance And Carcass Characteristics, Hannah L. Greenwell, Karla Jenkins, Jim C. Macdonald Donald, Matt K. Luebbe

Nebraska Beef Cattle Reports

The impact of field peas as a grazing supplement and a component of finishing diets on performance and carcass characteristics was evaluated over two years. During grazing, cattle supplemented with field peas had a greater ending body weight and average daily gain than cattle that received no supplement. However, cattle supplemented with corn had greater average daily gain than both peas and control cattle. Overall, those cattle not supplemented during grazing compensated 53% and 88% when compared to those cattle supplemented corn and peas, respectively. Inclusion of field peas in grower supplement or finishing diets may be advantageous if appropriately …

Effects Of Field Pea Supplementation On Digestibility And Rumen Volatile Fatty Acid Concentration Of Diets Containing High And Low Quality Forages, Hannah L. Greenwell, Jana L. Gramkow, Melissa L. Jolly-Breithaupt Breithaupt, Jim C. Macdonald, Karla Jenkins

Nebraska Beef Cattle Reports

Five ruminally cannulated steers were used to evaluate the effects of supplementation (no supplement, field peas, or dry rolled corn; 0.43% BW) with high and low quality forages on diet digestibility and rumen volatile fatty acid concentrations. Th e inclusion of field peas increased dry matter intake and organic matter digestibility over dry rolled corn and unsupplemented steers. Propionate proportions were less for field peas and control treatments than dry rolled corn, while acetate proportions increased in field peas, and control treatments as compared to dry rolled corn. As a result, acetate to propionate ratio was reduced when dry rolled …

Effects Of Wintering System On Cow And Calf Performance In A Summer- Calving Intensive Production System, Shelby E. Gardine, Jason M. Warner, Curtis J. Bittner Bittner, Robert G. Bondurant, Karla Jenkins, Richard J. Rasby, Matt K. Luebbe, Galen E. Erickson, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

A study evaluated the effects of two wintering systems (cornstalk grazing and drylot feeding) on cow- calf performance in a summer- calving intensively managed cowherd at two locations. Grazing cow- calf pairs on cornstalks resulted in lower ending BW of cows and reduced ADG of calves when compared to drylot cow- calf pairs at weaning. A partial budget of incorporating winter cornstalk grazing into an intensive production system suggests that cows wintered on cornstalks were $136.85 more profitable when compared to cows wintered in the drylot.

Effects Of Modified Distillers Grains And Corn Ratios As Supplements In Diets Varying In Forage Quality On Performance Of Growing Beef Steers, Viviane B. Ferrari, Robert G. Bondurant, Galen E. Erickson Erickson, Terry J. Klopfenstein, Jim C. Macdonald

Nebraska Beef Cattle Reports

Distillers grains (DG) have been intensively used for beef production, but prices relative to corn vary such that it may be beneficial to displace a portion of DG with corn in certain circumstances. Four ratios of supplemental energy (40% of diet DM) from modified distillers grains and corn (100:0, 80:20, 60:40, and 40:60) were supplemented in one of 3 forage diets (56% of diet DM) and evaluated for performance. Forages were high (brome hay + sorghum silage) or low quality. Low quality forages were corn residue baled through conventional rake and bale, or by disengaging the spreader on the combine …

Fatty Acid Composition Of Beef Fed Omnigen- Af At Receiving Or Finishing, Katherine I. Domenech, Michael D. Chao, Joe O. Buntyn Buntyn, Ty B. Schmidt, Chris Calkins

Nebraska Beef Cattle Reports

Beef fatty acid profiles and superoxide dismutase activity were determined for cattle receiving OmniGen- AF supplementation (a patented nutritional supplement) at receiving (first 28 d at the feedlot) or throughout finishing (all 210 d of finishing) vs. a control group (non- supplemented). Th e most meaningful change in fatty acid composition from inclusion of OmniGen- AF was total poly- unsaturated fatty acid (PUFA) content where beef from the finishing group had more PUFA content in relation to the receiving group and was not different from the control group. Despite this increase in PUFA, cattle supplemented through finishing tended to have …

Effect Of Backgrounding System On Steer Performance And Carcass Characteristics, Jordan L. Cox, Kristin E. Hales, Kristen M. Ulmer Ulmer, Richard J. Rasby, Steven D. Shackleford, Chad Engle, John Rieckman, Harvey C. Freetly, Mary Drewnoski

Nebraska Beef Cattle Reports

The impact of 3 backgrounding systems: grazing corn residue with distillers grains supplementation at 0.86% BW/d, grazing an oats-brassica forage, or feeding a grower ration in a drylot on finishing performance and carcass characteristics were evaluated. Backgrounding phase gains were greatest for steers fed a grower ration in the drylot (3.58 lb/d), intermediate for steers grazing oats-brassica forage and then fed the grower ration for short period (2.65 lb/d), and least for steers grazing corn residue while supplemented distillers grains and then fed the grower ration for short period (2.22 lb/d). These backgrounding treatment differences did not affect ADG during …

Shelf Life Of Ground Beef From Cattle Fed Distillers Grains Containing Different Amounts Of Oil, Faith D. Rasmussen, Chad G. Bower, Gary A. Sullivan Sullivan

Nebraska Beef Cattle Reports

Beef shoulder clods were collected from steers fed one of four finishing diets: no distillers grains and three with distillers grains containing different amounts of oil. Raw ground beef patties were evaluated for changes in objective color, discoloration, and lipid oxidation during simulated retail display (7 days). Cooked beef links in refrigerated (18 days) and frozen storage (198 days) were analyzed for lipid oxidation throughout shelf life. Fatty acid profiles were evaluated in lean, subcutaneous fat, and ground composite samples. All distillers grain diets increased C18:2 and polyunsaturated fatty acids in beef. There were no dietary differences in lipid oxidation …

Rumen Undegradable Protein And Bambermycins Supplementation Of Calves Grazing Corn Residue, Cody A. Welchons, Robert G. Bondurant, F. Henry Hilscher Hilscher, Jim C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

Steer calves grazing non- irrigated corn residue were supplemented with a corn residue and by- product pellet that contained either Soypass, soybean meal, or processed soybean meal at a rate of 4 lb / d. Additionally, a supplement was fed at 1 lb / d that provided 0 or 20 mg / steer daily of bambermycins (Gainpro®). There was no interaction between bambermycins inclusion and pellet type. Likewise, there was no effect of pellet type or bambermycins on ending BW or ADG. In order to maximize gain of calves grazing corn residue it is important to provide a supplement that …

Delineating Complex Forage Mixtures Using Plant-Wax Markers, Napoleón Vargas Jurado, Hannah C. Hamilton, Ronald M. Lewis

Nebraska Beef Cattle Reports

Plant waxes provide a marker profile of individual plants that, when sufficiently distinct, can be used to estimate the diet composition of grazing cattle. They also may provide a tool for reliably predicting feed intake. The traditional method (nonnegative least squares) to use these markers to predict diet composition has limitations. A newer statistical approach (Bayesian linear unmixing) deemed more efficient was tested with simulation. Samples from 8 forage species in Nebraska were analyzed for their plant- wax marker contents. Those concentrations were used to simulate 1000 diets for 4 mixtures containing 2, 3, 5 or 8 plants. The efficiency …

Effect Of Corn Residue Grazing Or Baling On Subsequent Crop Yield And Nutrient Removal, Kristen M. Ulmer, Jordan L. Cox, Manbir K. Rakkar Rakkar, Robert G. Bondurant, Humberto Blanco-Canqui, Mary Drewnoski, Karla H. Jenkins, James C. Macdonald, Rick J. Rasby

Nebraska Beef Cattle Reports

A 2- year study evaluated the effect of corn residue baling or grazing on subsequent yields, as well as, nutrient removal by baling at five locations in Nebraska. Three treatments were applied to each field: baled, grazed, and control (no baling and no grazing). Grain and stover yields were measured by hand harvest at maturity. No differences were observed among treatments for corn yield with baled having yields of 234 bu grain / acre, grazed with 239 bu grain / acre and control with 223 bu grain / acre. Th ere was no difference in stover yield among treatments (8,326, …

Timed Insemination Vs. Modified Estrus Detection In Beef Heifers, Benjamin T. Tibbitts, Tonya L. Meyer, Dan J. Kelly Kelly, Richard N. Funston

Nebraska Beef Cattle Reports

Th e objective of this study was to compare a modified estrus detection and fixed time AI vs. no estrus detection and fixed time AI on subsequent pregnancy rates. Yearling heifers were estrus synchronized and AI at 72 ± 2 h after prostaglandin injection. In one group estrus was not detected and all heifers received gonadotropin releasing hormone at the fixed- time AI; in the other group estrus was detected at 58 ± 2 and 70 ± 2 h after prostaglandin and inseminated in the following order at 72 ± 2 h: heifers in estrus at 58 h, heifers in …

Growth And Reproductive Performance Of Yearling Beef Heifers Implanted With Revalor G In The Nebraska Sandhills, Benjamin T. Tibbitts, Hazy Rae Nielson, K. H. Ramsay Ramsay, Richard N. Funston

Nebraska Beef Cattle Reports

Th is study evaluated effects of a single stocker implant (Revalor G) on growth and reproductive performance of yearling beef heifers in the Nebraska Sandhills. Crossbred heifers, grazing native Sandhills range, were randomly assigned to either be implanted 82 ± 2 days prior to estrus synchronization, or not implanted, to determine the effects of growth implants on heifer performance. Heifers were bred via artificial insemination followed with clean- up bulls. Implanted heifers gained more and were heavier at the end of the trial, but had a reduced pregnancy rate than non- implanted heifers. Implanted heifers also had a lower pregnancy …

Impact Of Heifer Development System In Two Different Breeding Seasons, Shelby A. Springman, Hazy Rae Nielson, Jacqueline A. Musgrave Musgrave, John Nollette, Andy Applegarth, Richard N. Funston

Nebraska Beef Cattle Reports

Replacement heifers from March and May calving herds were offered ad libitum meadow hay and 4 lb/d supplement or grazed meadow and offered 1 lb/d supplement from mid- January to mid- April. Heifers fed hay gained more during the treatment; however, heifers grazing meadow experienced compensatory gain, resulting in similar body weight at pregnancy diagnosis in both calving herds. Pregnancy rates were similar between treatment groups in March and May heifers. A reduced input winter management system is a viable option to maintain pregnancy rates in early and late summer breeding seasons.

Effects Of Distillers Grains Or Fiber On Enterohemorrhagic Escherichia Coli In Steers, Liesel G. Schneider, Curtis J. Bittner, Jordan E. Burhoop Burhoop, F. Henry Hilscher, Galen E. Erickson, Terry J. Klopfenstein, Rodney A. Moxley, David R. Smith

Nebraska Beef Cattle Reports

Increased probability for fecal shedding and colonization by Escherichia coli O157:H7 has been observed in previous studies when steers are fed increased inclusions of distillers grains. A feeding study was conducted to determine if distillers grains or fiber from distillers grains in the finishing ration of feedlot steers affected fecal shedding prevalence of seven serogroups of enterohemorrhagic Escherichia coli (EHEC- 7). For EHEC O45 and EHEC O103, the greatest prevalence of shedding occurred when steers were fed increased distillers grains For all EHECs except for O111, fecal shedding prevalence was similar between the corn control and either of the corn …

Effect Of Long- Term Corn Residue Grazing On Soil Properties, Manbir K. Rakkar, Humberto Blanco, Mary E. Drewnoski Drewnoski, Jim C. Macdonald, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

An experiment was conducted to evaluate the effect of long- term (16 years) corn residue grazing with cattle on soil compaction, soil structure, soil organic matter, and nutrients. Three treatments: 1) fall grazing (November through January; 1.8 to 2.5 AUM/ac), 2) spring grazing (February to middle April; 2.3 to 3.1 AUM/ac), and 3) control (no grazing) under a no- till irrigated corn- soybean system in eastern Nebraska were studied. Crop yields were increased and soil bulk density and cone index (parameters of soil compaction), wet soil aggregate stability (parameter of soil structural quality), and organic matter content were not affected …

Effect Of Increasing Supplemental Rumen Undegradable Protein (Rup) On Performance Of Calves Fed A Silage Growing Diet, Colton R. Oney, F. Henry Hilscher, Robert G. Bondurant Bondurant, Andrea K. Watson, Galen E. Erickson, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

A growing study evaluated the effects of increasing supplemental rumen undegradable protein (RUP) on performance of calves fed an 85% corn silage diet. Five levels of supplementation were evaluated with 12 individually fed steers per level of supplement. Supplement levels consisted of 0, 3.25, 6.5, 9.75 and 13% supplemental RUP (% of diet DM) fed as a blend of 60% Soypass and 40% Empyreal. Increasing supplemental RUP in the diet increased ending body weight and average daily gain linearly, and decreased F:G linearly while DMI remained constant among treatments. As cattle grew from 600 to 730 lb, F:G was improved …

2017 Nebraska Beef Cattle Report (Complete)

Nebraska Beef Cattle Reports

Contents

Cow/Calf: Effect of Heifer Development System on Reproduction and Subsequent Gain as a Pregnant Heifer • Impact of Heifer Development System in Two Breeding Seasons • Timed Insemination vs. Modifi ed Estrus Detection in Beef Heifers • Growth and Reproductive Performance of Yearling Beef Heifers Implanted with Revalor G in the Nebraska Sandhills • Development of Terminal and Maternal Economic Selection Indices in Beefmaster Cattle • Effects of Wintering System on Cow and Calf Performance in a Summer- Calving Intensive Production System • Methods to Increase Productivity of Spring Calving Production Systems in the Nebraska Sandhills

Growing: Effect of …

Dialectics Of Imagination And Experimentation: Basic Science Research In Developing Countries, Hr Ahmad, Na Khan

Department of Biological & Biomedical Sciences

No abstract provided.

Transposon-Mediated Stable Suppression Of Gene Expression In The Developing Chick Retina, Masaru Nakamoto, Chizu Nakamoto

Biology Faculty Publications

The embryonic chick has long been a favorite model system for in vivo studies of vertebrate development. However, a major technical limitation of the chick embryo has been the lack of efficient loss-of-function approaches for analyses of gene functions. Here, we describe a methodology in which a transgene encoding artificial microRNA sequences is introduced into embryonic chick retinal cells by in ovo electroporation and integrated into the genome using the Tol2 transposon system. We show that this methodology can induce potent and stable suppression of gene expression. This technique therefore provides a rapid and robust loss-of-function approach for studies of …

Influence Of Mature Overstory Trees On Adjacent 12-Year Regeneration And The Woody Understory: Aggregated Retention Versus Intact Forest, Miranda T. Curzon, Susan C. Baker, Christel C. Kern, Brian J. Palik, Anthony W. D'Amato

Aspen Bibliography

No abstract provided.

Loon Project Database, Walter H. Piper

Biology, Chemistry, and Environmental Sciences Faculty Data Sets

Data collected since 1993 in Oneida County, Wisconsin, on the breeding and territorial behavior of the common loon, Gavia immer. This study population is marked with USGS metal bands and colored leg bands for individual identification. It includes many individuals banded as chicks, whose life histories are known thoroughly. Data are collected from April through August of most years on this migratory species.

Exploring The Physics Of Proteins At Molecular Level By Neutron And X-Ray Scattering, Utsab Raj Shrestha

Wayne State University Dissertations

The protein structures revealed by the crystallographic studies have provided the valuable information over the years regarding their biological functions. However, such snapshots of protein fluctuations averaged over time may not be enough to fully capture the underlying biological phenomena. A deeper understanding of the protein dynamics is crucial for elucidating the structural pathways or the transition mechanism from the initial state to the final state necessary for regulating the physical and chemical processes. Hence, the biological activities and functions are mainly governed by the protein conformational dynamics. However, the direct correlation of a wide range of protein dynamics to …

Real-Time Investigation Of Bulky Lesion Bypass By Y-Family Dna Polymerase, Dpo4, Using Single Molecule Fret, Pramodha Liyanage

Wayne State University Dissertations

DNA is constantly exposed to various DNA damaging agents that are generated by various internal and external sources. Some of this damage may not be able to be repaired by cellular machineries causing DNA replication to be blocked. Once the replication fork is blocked by a DNA adduct, damage tolerance DNA polymerases, mainly Y-family, are able to restore the DNA replication by synthesizing past the DNA adduct. Benzo[a]pyrene (B[a]P) is one of the most studied environmental carcinogens. It is known to make covalent DNA adducts after metabolic activation and the bulkiness of the B[a]P adducts impose a strong barrier to …

Development Of A Finite Element Pelvis And Lower Extremity Model With Growth Plates For Pediatric Pedestrian Protection, Ming Shen

Wayne State University Dissertations

Finite element (FE) model is a useful tool frequently used for investigating the injury mechanisms and designing protection countermeasures. At present, no 10 years old (YO) pedestrian FE model has been developed from appropriate anthropometries and validated against limitedly available impact response data. A 10 YO child FE pelvis and lower extremities (PLEX) model was established to fill the gap of lacking such models in this age group. The baseline model was validated against available pediatric postmortem human subjects (PMHS) test data and additional scaled adult data, then the PLEX model was integrated to build a whole-body FE model representing …

The Vegetation And Flora Of Edwards Air Force Base, Western Mojave Desert, California, David Charlton, Philip W. Rundel

Aliso: A Journal of Systematic and Floristic Botany

Edwards Air Force Base extends over 121,000 ha in the Antelope Valley of the western Mojave Desert, with much of the area part of a closed endorheic basin that held the Pleistocene Lake Thompson. Notable topographic features are Rogers, Rosamond and Buckhorn dry lakes, while rounded domes and scattered hills are present to the north and east. Elevation gradients are limited, ranging from a low of 690 m to 1044 m near the eastern margin. Diverse communities of saltbush scrub dominate the lower plains, while creosote bush scrub and Joshua tree woodlands are present away from the old lake basin. …