Life Sciences Commons^™

Phosphorylation Of Cytochrome C Threonine 28 Regulates Electron Transport Chain Activity In Kidney: Implications For Amp Kinase, Gargi Mahapatra, Ashwathy Varughese, Qinqin Ji, Icksoo Lee, Jenney Liu, Asmita Vaishnav, Christopher Sinkler, Alexandr A. Kapralov, Carlos T. Moraes, Thomas H. Sanderson, Timothy L. Stemmler, Lawrence I. Grossman, Valerian E. Kagan, Joseph S. Brunzelle, Arthur R. Salomon, Brian Fp Edwards, Maik HüTtemann

Biochemistry and Molecular Biology Faculty Publications

Mammalian cytochrome c (Cytc) plays a key role in cellular life and death decisions, functioning as an electron carrier in the electron transport chain and as a trigger of apoptosis when released from the mitochondria. However, its regulation is not well understood. We show that the major fraction of Cytc iso- lated from kidneys is phosphorylated on Thr28, leading to a par- tial inhibition of respiration in the reaction with cytochrome c oxidase. To further study the effect of Cytc phosphorylation in vitro, we generated T28E phosphomimetic Cytc, revealing supe- rior behavior regarding protein stability and its ability to degrade …

Comparative Postembryonic Skeletal Ontogeny In Two Sister Lineages Of Old World Tree Frogs (Rhacophoridae: Taruga, Polypedates), Gayani Senevirathne, Ryan R. Kerney, Madhava Meegaskumbura

Biology Faculty Publications

Rhacophoridae, a family of morphologically cryptic frogs, with many genetically distinct evolutionary lineages, is understudied with respect to skeletal morphology, life history traits and skeletal ontogeny. Here we analyze two species each from two sister lineages, Taruga and Polypedates, and compare their postembryonic skeletal ontogeny, larval chondrocrania and adult osteology in the context of a well-resolved phylogeny. We further compare these ontogenetic traits with the direct-developing Pseudophilautus silus. For each species, we differentially stained a nearly complete developmental series of tadpoles from early postembryonic stages through metamorphosis to determine the intraspecific and interspecific differences of cranial and postcranial …

A Cationic Amphiphilic Random Copolymer With Ph-Responsive Activity Against Methicillin-Resistant Staphylococcus Aureus., Sungyoup Hong, Haruko Takahashi, Enrico T Nadres, Hamid Mortazavian, Gregory Caputo, John G Younger, Kenichi Kuroda

College of Science & Mathematics Departmental Research

In this report, we demonstrate the pH-dependent, in vitro antimicrobial activity of a cationic, amphiphilic random copolymer against clinical isolates of drug-resistant Staphylococcus aureus. The polymer was developed toward a long-term goal of potential utility in the treatment of skin infections. The proposed mechanism of action of the polymer is through selectively binding to bacterial membranes and subsequent disruption of the membrane structure/integrity, ultimately resulting in bacterial cell death. The polymer showed bactericidal activity against clinical isolates of methicillin-resistant or vancomycin-intermediate S. aureus. The polymer was effective in killing S. aureus at neutral pH, but inactive under acidic conditions (pH …

Cognition-Mediated Evolution Of Low-Quality Floral Nectars, Vladislav Nachev, Kai Petra Stich, Clemens Winter, Alan B. Bond, Alan Kamil, York Winter

Alan Bond Publications

Plants pollinated by hummingbirds or bats produce dilute nectars even though these animals prefer more concentrated sugar solutions. This mismatch is an unsolved evolutionary paradox. Here we show that lower quality, or more dilute, nectars evolve when the strength of preferring larger quantities or higher qualities of nectar diminishes as magnitudes of the physical stimuli increase. In a virtual evolution experiment conducted in the tropical rainforest, bats visited computer-automated flowers with simulated genomes that evolved relatively dilute nectars. Simulations replicated this evolution only when value functions, which relate the physical stimuli to subjective sensations, were nonlinear. Selection also depended on …

Predictive Screening Of M1 And M2 Macrophages Reveals The Immunomodulatory Effectiveness Of Post Spinal Cord Injury Azithromycin Treatment, John C. Gensel, Timothy J. Kopper, Bei Zhang, Michael B. Orr, William M. Bailey

Spinal Cord and Brain Injury Research Center Faculty Publications

Spinal cord injury (SCI) triggers a heterogeneous macrophage response that when experimentally polarized toward alternative forms of activation (M2 macrophages) promotes tissue and functional recovery. There are limited pharmacological therapies that can drive this reparative inflammatory state. In the current study, we used in vitrosystems to comprehensively defined markers of macrophages with known pathological (M1) and reparative (M2) properties in SCI. We then used these markers to objectively define the macrophage activation states after SCI in response to delayed azithromycin treatment. Mice were subjected to moderate-severe thoracic contusion SCI. Azithromycin or vehicle was administered beginning 30 minutes post-SCI and …

Metastable Pain-Attention Dynamics During Incremental Exhaustive Exercise, Agnė Slapšinskaitė, Robert Hristovski, Selen Razon, Natàlia Balagué, Gershon Tenenbaum

Kinesiology Faculty Publications

No abstract provided.

Genome Editing Of Wnt-1, A Gene Associated With Segmentation, Via Crispr/Cas9 In The Pine Caterpillar Moth, Dendrolimus Punctatus, Huihui Liu, Qun Liu, Xuguo Zhou, Yongping Huang, Zhen Zhang

Entomology Faculty Publications

The pine caterpillar moth, Dendrolimus punctatus, is a devastating forest pest. Genetic manipulation of this insect pest is limited due to the lack of genomic and functional genomic toolsets. Recently, CRISPR/Cas9 technology has been demonstrated to be a promising approach to modify the genome. To investigate gene functions during the embryogenesis, we introduced CRISPR/Cas9 system in D. punctatus to precisely and effectively manipulate gene expressions inmutant embryos. Compared to controls, knocking out of DpWnt-1, a gene well known for its role in the early body planning, led to high embryonic mortality. Among these mutants, 32.9% of the embryos …

Eccentric Resistance Training In Adults With And Without Spinal Cord Injuries, Whitley J. Stone, Sandra L. Stevens, Dana K. Fuller, Jennifer L. Caputo

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 154-165, 2017 The purpose of this study was to examine the effects of active lower body eccentric resistance training (ERT) in individuals with incomplete spinal cord injury (iSCI) and controls (CON). Specifically, the study was designed to determine if those with iSCI adapt similarly to ERT as CON participants as well as the overall safety and efficacy of ERT in this population. This pilot investigation involved the recruitment of persons with iSCI (n = 3) and age- and sex-matched able-bodied CON (n = 3). The 8-week intervention focused on building lower extremity …

The Accuracy Of Pedometers In Measuring Walking Steps On A Treadmill In College Students, Hannah M. Husted, Tamra Llewellyn

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 146-153, 2017. Pedometers are a popular way for people to track if they have reached the recommended 10,000 daily steps. Therefore, the purpose of this study was to determine the accuracy of four brands of pedometers at measuring steps, and to determine if a relationship exists between pedometer cost and accuracy. The hypothesis was that the more expensive brands of pedometers (the Fitbit Charge™ and Omron HJ-303™) would yield more accurate step counts than less expensive brands (the SmartHealth – Walking FIT™ and Sportline™). While wearing all pedometers at once, one male and eleven …

The Effect Of High Intensity Interval Run Training On Cross Sectional Area Of The Vastus Lateralis In Untrained College Students, Rebekah Estes, David Thrush, Amy Malinowski, Meredith Piacentini, Eric Salley, Cassidy Losey, Erik Hayes

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 137-145, 2017 Aerobic cycling has been repeatedly shown to induce hypertrophy in skeletal muscle across a variety of populations, while there has been a lack of investigation into the impact of running upon hypertrophy. An increasingly popular model of aerobic exercise is high-intensity interval training (HIIT); in addition to its positive impact upon cardiovascular health, HIIT may be sufficient for inducing significant muscular hypertrophy. Therefore, the purpose of this investigation was to examine the influence of a high-intensity interval running protocol upon hypertrophy of the vastus lateralis in an untrained, young population. Twelve recreationally …

Effects Of Simulated Altitude On Maximal Oxygen Uptake And Inspiratory Fitness, Nicole Biggs, Benjamin England, Nicole Turcotte, Melissa R. Cook, Alyne L. Williams

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 128-136, 2017 Aerobic exercise at altitude has shown an increase in maximal oxygen uptake. Similar effects have been replicated by way of simulated altitude training, which have influenced various advances in the field of exercise science. Elevation Training Masks© (ETM) claim to stimulate cardiorespiratory fitness improvements similar to training at altitude, however, there is little research to support this claim. The purpose of this study was to research the effect that a hypoxia-inducing mask would have on cardiorespiratory fitness and pulmonary function through the use of a high intensity interval training (HIIT) running program. …

Meeting Usdhhs Physical Activity Guidelines And Health Outcomes, E. Earlynn Lauer, Allen W. Jackson, Scott B. Martin, James R. Morrow Jr.

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 121-127, 2017 Current public health physical activity (PA) guidelines suggest ≥500 METmin/week of PA, with additional benefits beyond 1000 METmin/week (i.e., a dose response). Revised U.S. PA guidelines are scheduled for 2018. The purpose was to relate health markers (blood pressure, percent fat, BMI, blood glucose, cholesterol, and cardiorespiratory fitness) to verify the dose response for PA guidelines revision. 505 non-smoking participants self-reported PA behaviors and completed medical screening. MANCOVA controlling for age and gender determined the relation between health markers and PA. MANCOVA indicated significantly (P<.001) different health markers of percent fat, BMI, glucose, and treadmill time as a function of PA. Post-hoc Helmert contrasts (1] <500 METmin/week vs. ≥500 METmin/week and 2] 500 to <1000 METmin/week vs. ≥1000 METmin/week) indicated that meeting the PA guidelines was associated with better health markers and higher cardiorespiratory fitness. Effect sizes were greater for contrast 1 than for contrast 2, suggesting a plateauing effect. Revised public health guidelines should consider achievement of ≥500 METmin/week to be most important, with additional modest gain thereafter. Revised PA guidelines should stress the importance of achieving 500-1000 METmin/week.

Acute Effects Of 24-H Sleep Deprivation On Salivary Cortisol And Testosterone Concentrations And Testosterone To Cortisol Ratio Following Supplementation With Caffeine Or Placebo, Ciaran K. Mc Donald, Joss Moore, Alan Mc Intyre, Kevin Carmody, Bernard Donne

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 108-120, 2017. Caffeine has become a popular ergogenic aid amongst athletes and usage to improve athletic performance has been well documented. The effect of caffeine on anabolic and catabolic hormones in a sleep-deprived state has had little investigation to date. The purpose of the current study was to investigate the potential of caffeine to offset the effects, if any, of short-term sleep deprivation and exercise on an athlete’s testosterone and cortisol concentrations via salivary technique. Eleven competitive male athletes volunteered to be part of this prospective double-blinded study. Three test days were scheduled for …

The Effects Of A 10-Day Altitude Training Camp At 1828 Meters On Varsity Cross-Country Runners, Sebastian R. Diebel, Ian Newhouse, David S. Thompson, Vineet Bk Johnson

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 97-107, 2017. Altitude training has been shown to alter blood lactate (BL) levels due to alterations resulting from acclimatization. This study aims to estimate the impact of altitude training on BL changes immediately following an incremental treadmill test and during recovery before and after 10-day altitude training at approximately 1828 meters. Eight varsity cross-country runners performed an incremental treadmill test (ITT), pre and post-altitude training. Resting and post-warm-up BL values were recorded. During ITT, heart rate (HR), oxygen saturation (SpO2), and time to exhaustion were monitored. BL was also measured post-ITT at 0, 2, …

Validity Of The Bottle Buoyancy Model For Body Fat Determination, Cherie Pettitt, Kalvin Danzl, Lauren M. Glorvigen, Cody J. Strom, Thomas A. Inkrott, Robert W. Pettitt

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 87-96, 2017. We investigated a modification of the bottle buoyancy (BB) method in comparison to single frequency, bioelectric impedance analysis (BIA) as a valid noninvasive method of percent body fat (%BF) determination. Twenty-eight participants (15 men, 13 women), in counterbalanced-order, completed the BB, BIA, and computerized hydrostatic densitometry (HD) methods. We elected to modify the BB method using a 12.15 L container with participants hugging the container in an upright position. Consistency measures of intraclass correlation coefficient (ICC), typical error (TE), coefficient of variation (CV) and total error of measurement (TEM) are reported. Our …

Borg Scale Is Valid For Ratings Of Perceived Exertion For Individuals With Parkinson’S Disease, Amanda Penko

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 76-86, 2017. Parkinson’s disease is a neurodegenerative disease that has traditionally been treated with anti-parkinsonian medication. There is increasing evidence that exercise is beneficial to those with PD, therefore, it is necessary to validate a measure of exertion that can be implemented across exercise settings that may not have the capability to actively monitor heart rate. The aim of this project was to determine the validity of the Borg RPE scale in individuals with PD undergoing a maximal progressive cycling exercise test. Thirty-eight males and females (58.5 ± 8.1 yrs) with a clinical diagnosis …

Assessing Energy Level As A Marker Of Aerobic Exercise Readiness: A Pilot Investigation, Kelley Strohacker, William R. Boyer, Kayla N. Smitherman, Emily Cornelius, Daniel Fazzino

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 62-75, 2017. Energy ratings have been used as a marker of exercise readiness (i.e. pre-exercise physical/mental state indicating ability to perform) within flexible nonlinear periodization (FNLP)-based resistance training interventions. However, empirical data is lacking regarding the utility of this approach for aerobic exercise. The purpose of this study was to examine the ability of pre-exercise energy level to predict affective and behavioral responses to prescribed aerobic exercise. Participants consisted of 19 women and 8 men (N=27, age=20±4 years, estimated maximal oxygen uptake=37±6). Participants performed two 30-min bouts of treadmill exercise under an imposed moderate …

The Acute Effects Of Static Stretching Compared To Dynamic Stretching With And Without An Active Warm Up On Anaerobic Performance, Bradley J. Kendall

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 53-61, 2017. The Wingate Anaerobic Test (WAnT) has been used in many studies to determine anaerobic performance. However, there has been poor reporting of warm-up protocols and limited consistency between warm-up methods that have been used. With the WAnT being such a commonly-used test, consistency in warm-up methods is essential in order to compare results across studies. Therefore, this study was designed to compare how static stretching, dynamic stretching, and an active warm-up affect WAnT performance. Ten recreationally active participants (5 males, 5 females) with a mean (SD) age of 23.3 (0.7) volunteered for …

The Relationship Between Sports Specialization And Mental Toughness, Courtney Buhrow, Jacob Digmann, Jennifer J. Waldron

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 44-52, 2017. The pros and cons of early sport specialization compared to diversification have been examined in many research studies. The purpose of this study was to determine (a) the relationship between mental toughness and age of specialization in sport, and (b) differences in mental toughness based on early specialization of sport and gender. College athletes (N = 102) completed surveys about specialization and mental toughness, including MeBTough. The mean age of specialization was 13.45 (± 4.47). Results showed no significant difference in mental toughness of those who specialized early and those who did …

The Effects Of An Elevation Training Mask On Vo2max Of Male Reserve Officers Training Corps Cadets, Brian G. Warren, Frank Spaniol, Randy Bonnette

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 37-43, 2017. The purpose of this study was to investigate the effects of an elevation training mask (ETM) on the VO2max of male Reserve Officers Training Corps (ROTC) cadets. Fourteen male ROTC cadets (age 20.00 ± 1.8 yrs, height 174.35 cm ± 3.1 cm, weight 76.75 kg ± 11.09 kg, body fat 13.88% ± 4.62%) participated in this study to determine if an ETM would cause a significant increase VO2max. After the familiarization period, the test subjects were randomly assigned to either the control or experimental group, respectively. The training period lasted seven weeks …

The Effect Of Military Load Carriage On Postural Sway, Forward Trunk Lean, And Pelvic Girdle Motion, Eileen M. Strube, Andrea Sumner, Roger O. Kollock Jr, Kenneth E. Games, Marie A. Lackamp, Masahiro Mizutani, Joellen M. Sefton

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 25-36, 2017. Musculoskeletal injuries are a common occurrence in military service members. It is believed that the load carried by the service member impedes stability and alters back and pelvis kinematics, increasing their susceptibility to musculoskeletal injuries, specifically in the lower extremities. The purpose of this study was to examine the effects of two different loads on postural sway, forward trunk lean, and pelvic girdle motion in United States Army Cadets. Twenty male Army Reserve Officers’ Training Corps Cadets participated in this study. Each participant performed the Modified Clinical Testing of Sensory Interaction (mCTSIB) …

Sport Commitment Among Adult Recreational Soccer Players: Test Of An Expanded Model, Amanda L. Frayeh, Beth Lewis

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 4-24, 2017. Adult sport leagues provide opportunities for physical activity (PA), but little is known about adults’ commitment to recreational sports. We proposed two expanded versions of Scanlan and colleagues’ sport commitment model (SCM) to explore psychosocial factors related to adults’ participation in these leagues. Model 1 proposed athletic identity and social cohesion as additional predictors of commitment. Previous research found these variables to be positively related to commitment, but had not systematically examined them within the context of the SCM. We also explored participation in moderate-to-vigorous physical activity (MVPA) as a behavioral manifestation …

2016 International Journal Of Exercise Science Reviewer List

International Journal of Exercise Science

2016 International Journal of Exercise Science Reviewer List

International Journal of Exercise Science 10(1): 1-3, 2017

Phospholipase Dζ Enhances Diacylglycerol Flux Into Triacylglycerol, Wenyu Yang, Geliang Wang, Jia Li, Philip Bates, Xuemin Wang, Douglas Allen

Biology Department Faculty Works

Plant seeds are the primary source of triacylglycerols (TAG) for food, feed, fuel, and industrial applications. As TAG is produced from diacylglycerol (DAG), successful engineering strategies to enhance TAG levels have focused on the conversion of DAG to TAG. However, the production of TAG can be limited by flux through the enzymatic reactions that supply DAG. In this study, two Arabidopsis phospholipase Dζ genes (AtPLDζ1 and AtPLDζ2) were coexpressed in Camelina sativa to test whether the conversion of phosphatidylcholine to DAG impacts TAG levels in seeds. The resulting transgenic plants produced 2% to 3% more TAG as a component of …

Passerine Morphology: External Measurements Of Approximately One-Quarter Of Passerine Bird Species, Robert Ricklefs

Biology Department Faculty Works

Studies of community organization and clade diversification that include functional traits have become an important component of the analysis of ecological and evolved systems. Such studies frequently are limited by availability of consistently collected data. Here, I present a data set including eight measurements of the external morphology of 1642 species, roughly one‐quarter of all passerine birds (Aves: Order Passeriformes), from all parts of the world, characterizing the relative proportions of the wing, tail, legs, and beak. Specimens were measured opportunistically over the past 40 years in museums in the United States and Europe. Numbers of individuals measured per species …

Repealing The Aca Without A Replacement — The Risks To American Health Care, Barack Obama

United States Public Health Resources

Health care policy often shifts when the country’s leadership changes. That was true when I took office, and it will likely be true with President-elect Donald Trump. I am proud that my administration’s work, through the Affordable Care Act (ACA) and other policies, helped millions more Americans know the security of health care in a system that is more effective and efficient. At the same time, there is more work to do to ensure that all Americans have access to high-quality, affordable health care. What the past 8 years have taught us is that health care reform requires an evidence-based, …

Effects Of An Unusual Poison Identify A Lifespan Role For Topoisomerase 2 In Saccharomyces Cerevisiae, Gregory Tombline, Jonathan Millen, Bogdan Polevoda, Matan Rapaport, Bonnie Baxter, Michael Van Meter, Matthew Gilbertson, Joe Madrey, Gary A. Piazza, Lynn Rasmussen, Kirster Wennerberg, E. Lucile White, John L. Nitiss, David S. Goldfarb

Biology Faculty/Staff Publications

A progressive loss of genome maintenance has been implicated as both a cause and consequence of aging. Here we present evidence supporting the hypothesis that an age-associated decay in genome maintenance promotes aging in Saccharomyces cerevisiae (yeast) due to an inability to sense or repair DNA damage by topoisomerase 2 (yTop2). We describe the characterization of LS1, identified in a high throughput screen for small molecules that shorten the replicative lifespan of yeast. LS1 accelerates aging without affecting proliferative growth or viability. Genetic and biochemical criteria reveal LS1 to be a weak Top2 poison. Top2 poisons induce the accumulation of …

Implementing Comprehensive School Physical Activity Programs: A Wayne State University Case Study, Erin E. Centeio, Nate Mccaughtry

Kinesiology, Health and Sport Studies

Comprehensive school physical activity programs (CSPAPs) have been highlighted by numerous public health and education agencies for their potential to improve the health and academic achievement of American youth. A CSPAP integrates physical activity throughout the school environment before, during and after school by engaging educators, children, families and community organizations. As the design, implementation and evaluation of effective CSPAP programs has inched forward, so has the call for physical education teacher education (PETE) programs to prepare the next generation of physical educators with the skills and knowledge needed to one day lead CSPAP efforts in K-12 schools. The purpose …

Peixoto Studies, Teaches The Powerhouses Of Life., Aldemaro Romero Jr.

Publications and Research

Sometimes great things come from small ones. Among the smallest structures we find in almost all cells are the mitochondria. Not only are they very small (about 0.001of an inch), but they are also very numerous (some of our cells have more than 2,000 of them each). And their role in life is enormous: they generate the energy molecules we use to make our bodies work.

Pablo Peixoto studies these microscopic structures. Born in Brasilia, the capital of Brazil, he became interested in science early in life. “I was one of those annoying kids who always wanted to know the …

A Comparative Evaluation Of Gait Between Children With Autism And Typically Developing Matched Controls, Janet S. Dufek, Jeffrey D. Eggleston, John R. Harry, Robbin Hickman

Integrated Health Sciences Faculty Research

Anecdotal reports suggest children with autism spectrum disorder (ASD) ambulate differently than peers with typical development (TD). Little empirical evidence supports these reports. Children with ASD exhibit delayed motor skills, and it is important to determine whether or not motor movement deficits exist during walking. The purpose of the study was to perform a comprehensive lower-extremity gait analysis between children (aged 5–12 years) with ASD and age- and gender-matched-samples with TD. Gait parameters were normalized to 101 data points and the gait cycle was divided into seven sub-phases. The Model Statistic procedure was used to test for statistical significance between …