Life Sciences Commons^™

A Comparison Of Water Potential, Photosynthetic Rate, Electron Transport Rate, And Stomatal Conductance Between Native Malosma Laurina And Exotic Schinus Molle, Karagan L. Smith, Agatha C. Heng, Viridiana Hernandez-Lopez

Seaver College Research And Scholarly Achievement Symposium

A Comparison of Water Potential, Photosynthetic Rate, Electron Transport Rate, and Stomatal Conductance between Native Malosma laurina and Exotic Schinus molle

Karagan L. Smith, Agatha C. Heng, and Viridiana Hernandez-Lopez

First-year Students as Scholars Program

Mentors: Natalie M. Aguirre, Kaitlyn E. Sauer, and Stephen D. Davis

Schinus molle is a relatively new invasive species in chaparral shrub communities of the Santa Monica Mountains, and has only recently been observed to displace native species of shrubs, such as Malosma laurina. To investigate the probably cause of S. molle’s invasiveness and mechanisms of competitive displacement of M. laurina, we compared …

Progression Of Activity And Predator Avoidance In California Grunion Larvae, Callyn E. Shelley

Seaver College Research And Scholarly Achievement Symposium

No abstract provided.

Carbon Black Exposure Induces Alterations In Mitochondrial Morphology In Human Lung Cells: A Software-Based Quantitative Analysis, Jennifer D. Duff, Brianna K. Laferney, Celina Y. Jones, Penn M. Mcclatchey, Jay Brewster

Seaver College Research And Scholarly Achievement Symposium

Nanoparticulates of pure carbon, carbon black (CB), are a common atmospheric pollutant in industrialized and heavily populated areas. They are produced primarily via combustion of fossil fuels, and represent a significant health hazard. They are known to worsen asthma and bronchitis when inhaled and to cause inflammation, heart dysfunction, and oxidative stress when incorporated into other organs. The key focal point of this work was to examine markers of stress signaling and cellular dysfunction when human bronchial epithelial cells (16HBE14o-) were exposed to CB particles ranging in size from 70 nm and averaging 130 nm in diameter. BrdU incorporation and …

The Effect Of Water Levels On Crayfish Aggression, Emory B. Johnson, Sarah E. Blase

Seaver College Research And Scholarly Achievement Symposium

Crayfish are a very prevalent invasive species that affect many different environments all over the world. Crayfish, specifically Procambarus clarkii, are an invasive species that have currently taken a foothold in the Santa Monica mountains and are threatening native stream species. There is currently a severe drought occurring in the Santa Monica mountains. The drought has been reshaping many aquatic environments by limiting space and resources such as food and shelter. Crayfish are a very aggressive species and adversely affect the habitat they inhabit by rapidly colonizing, burrowing, and their voracious diets. Due to the adverse effects crayfish can have …

The North American Whistling-Ducks, Pochards, And Stifftails, Paul A. Johnsgard

Zea E-Books Collection

Although the 12 species representing three waterfowl tribes described in this volume are not closely related, they fortuitously provide an instructive example of adaptive evolutionary radiation within the much larger waterfowl lineage (the family Anatidae), especially as to their divergent morphologies, life histories, and social behaviors.

The whistling-ducks (Dendrocygna), with three known North American species, are notable for their permanent pair-bonds, extended biparental family care, and strong social cohesion. In contrast, males of the five typical pochards of North American diving ducks (Aythya) establish monogamous pair-bonds that are maintained only long enough to assure that the female’s eggs are fertilized. …

Abundance And Habitat Preferences Of Introduced Muscovy Ducks (Cairina Moschata), Jacqueline Marie Perry Cahanin

USF Tampa Graduate Theses and Dissertations

Muscovy ducks are native only to Central and South America, Mexico, and parts of southern Texas and are considered invasive in some areas outside of their native range. Although they have been introduced worldwide, they remain largely unstudied. The primary focus of this study was to relate Muscovy duck abundance to habitat characteristics of wetlands in Tampa, Florida. Muscovy abundance was measured using point count methods at 21 wetland sites that occur within an eight km radius of the University of South Florida’s main campus. Habitat features at these sites were assessed using field methods and Geographic Information Systems (GIS) …

Social Amoebae Mating Types Do Not Invest Unequally In Sexual Offspring, T E. Douglas, David C. Queller, Joan E. Strassmann

Biology Faculty Research

Unequal investment by different sexes in their progeny is common and includes differential investment in the zygote and differential care of the young. The social amoeba Dictyostelium discoideum has a sexual stage in which isogamous cells of any two of the three mating types fuse to form a zygote which then attracts hundreds of other cells to the macrocyst. The latter cells are cannibalized and so make no genetic contribution to reproduction. Previous literature suggests that this sacrifice may be induced in cells of one mating type by cells of another, resulting in a higher than expected production of macrocysts …

A Novel Approach To The Discovery Of Natural Products From Actinobacteria, Rahmy Tawfik

USF Tampa Graduate Theses and Dissertations

Actinobacteria, primarily the genus Streptomyces, have led to the development of a number of antibiotics, which result from their secondary metabolites or modified derivatives. Secondary metabolite production can result from competition with neighboring microbes in an effort to disrupt growth, aiding in the competition for vital nutrients in impoverished conditions. Such secondary metabolites have the potential to affect a plethora of cellular functions in target cells, including, cell wall development, protein synthesis, protein function and fatty acid synthesis/metabolism. Due to the pandemic spread of antibiotic resistant bacteria, it is imperative to continue the search for new therapeutic agents …

Distribution And Conservation Status Of The Freshwater Gastropods Of Nebraska, Bruce J. Stephen

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

This survey of freshwater gastropods within Nebraska includes 159 sample sites and encompasses the four primary level III ecoregions of the State. I identified sixteen species in five families. Six of the seven species with the highest incidence, Physa gyrina, Planorbella trivolvis, Stagnicola elodes, Gyraulus parvus, Stagnicola caperata, and Galba humilis were collected in each of Nebraska’s four major level III ecoregions. The exception, Physa acuta, was not collected in the Western High Plains ecoregion. Seven indigenous species, Valvata tricarinata, Helisoma anceps, Campeloma decisum, Galba bulimoides, Physa jennessi, Ferrissia rivularis, and …

Analysis Of Antibody-Induced Plasmodium Falciparum Sporozoites Through Scanning Electron Microscopy, Sagorika Bera

USF Tampa Graduate Theses and Dissertations

Malaria is a devastating disease that continues to affect millions of people worldwide every year. Specifically, Plasmodium falciparum is the most common human malaria parasite, particularly in sub-Saharan Africa. P. falciparum causes the most malignant and debilitating symptoms with the highest mortality and complication rates. Even with the worldwide efforts of many researchers and organizations, the road to discovering a vaccine has been difficult and challenging. Due do to the improvements in in vitro liver stage assays as well as rodent models of mammalian malaria, pre-erythrocytic stages of malaria have become a more accessible target for experimental studies. These vaccine …

Nematocyst Distribution Corresponds To Prey Capture Location In Hydromedusae With Different Predation Modes, Marco Corrales-Ugalde, Sean Colin, Kelly R. Sutherland

Arts & Sciences Faculty Publications

Understanding the factors that control predation in pelagic communities can inform predictions of community structure in marine ecosystems. Ubiquitous and selective predators such as cnidarian hydromedusae rely on their nematocysts to capture and retain prey but it is not clear how the density and spatial distribution of these cells relate to predation mode. We examined the relationship between prey capture and nematocyst distribution in the tentacles of Aglantha digitale and Proboscidactyla flavicirrata, which are considered ambush predators, and Clytia gregaria and Mitrocoma cellularia, which are considered feeding-current predators. First, we analyzed video of predator-prey interactions to compare capture locations of …

Ang1 Treatment Reduces Muscle Pathology And Prevents A Decline In Perfusion In Dmd Mice, Kelly M. Gutpell, Nikola Tasevski, Boaz Wong, William Thomas Hrinivich, Feng Su, Lise Desjardins, Ting-Yim Lee, Lisa Marie Hoffman

Anatomy and Cell Biology Publications

Vascular endothelial growth factor (VEGF) and other pro-angiogenic growth factors have been investigated to enhance muscle tissue perfusion and repair in Duchenne muscular dystrophy (DMD). Current understanding is limited by a lack of functional data following in vivo delivery of these growth factors. We previously used dynamic contrast-enhanced computed tomography to monitor disease progression in murine models of DMD, but no study to date has utilized this imaging technique to assess vascular therapy in a preclinical model of DMD. In the current study, we locally delivered VEGF and ANG1 alone or in combination to dystrophic hind limb skeletal muscle. Using …

Hydrogen Peroxide Inhibition And Inactivation Of Bicupin Oxalate Oxidase, John M. Goodwin Vii

Master of Science in Chemical Sciences Theses

Oxalate oxidase is a manganese containing enzyme that catalyzes the oxygen dependent oxidation of oxalate to carbon dioxide and hydrogen peroxide. Oxalate oxidase from Ceriporiopsis subvermispora (CsOxOx) is the first characterized bicupin oxalate oxidase. Potential applications of oxalate oxidase in cancer treatment, paper pulping, and biofuel cells highlight the need to study the effects of hydrogen peroxide on the CsOxOx catalyzed oxidation of oxalate. Membrane inlet mass spectrometry (MIMS) was used to directly measure initial rates of carbon dioxide formation and oxygen consumption in varying initial concentrations of hydrogen peroxide. This work demonstrates that hydrogen peroxide is both a reversible …

Tiered Vs. Traditional Daily Undulating Periodization For Improving Powerlifting Performance In Trained Males, Andres Vargas

USF Tampa Graduate Theses and Dissertations

Daily undulating periodization represents an increasingly popular trend in exercise science with which a traditional model has been established. Tiered daily undulating periodization pairs the DUP approach with a tiered training system which allows volume and intensity to be more evenly distributed throughout a given graining cycle. The concept of tiered daily undulating periodization is a novel form of periodization and has not been investigated. As such, a comparison of traditional and tiered daily undulating periodization has yet to be examined. Therefore, the purpose of this study was to compare the effects of traditional and tiered daily undulating periodization models …

Complete Genome Sequences Of Pseudomonas Fluorescens Bacteriophages Isolated From Freshwater Samples In Omaha, Nebraska, Guoqing Lu, Jamie L. Luhr, Andrew Stoecklein, Paige Warner, William Tapprich

Biology Faculty Publications

The complete genome sequences of four Pseudomonas fluorescens bacteriophages, UNO-SLW1 to UNO-SLW4, isolated from freshwater samples, are 39,092 to 39,215 bp long. The genomes are highly similar (identity, >0.995) but dissimilar from that of Pseudomonas phage Pf-10 (the closest relative, 0.685 to 0.686 identity), with 48 to 49 protein-coding genes and 66 regulatory sites predicted.

Virus-Mediated Suppression Of Host Non-Self Recognition Facilitates Horizontal Transmission Of Heterologous Viruses, Songsong Wu, Jiasen Cheng, Yanping Fu, Tao Chen, Daohong Jiang, Said A. Ghabrial, Jiatao Xie

Plant Pathology Faculty Publications

Non-self recognition is a common phenomenon among organisms; it often leads to innate immunity to prevent the invasion of parasites and maintain the genetic polymorphism of organisms. Fungal vegetative incompatibility is a type of non-self recognition which often induces programmed cell death (PCD) and restricts the spread of molecular parasites. It is not clearly known whether virus infection could attenuate non-self recognition among host individuals to facilitate its spread. Here, we report that a hypovirulence-associated mycoreovirus, named Sclerotinia sclerotiorum mycoreovirus 4 (SsMYRV4), could suppress host non-self recognition and facilitate horizontal transmission of heterologous viruses. We found that cell death in …

A Novel Multi-Network Approach Reveals Tissue-Specific Cellular Modulators Of Fibrosis In Systemic Sclerosis, Jaclyn N. Taroni, Casey S. Greene, Viktor Martyanov, Tammara A. Wood

Dartmouth Scholarship

Systemic sclerosis (SSc) is a multi-organ autoimmune disease characterized by skin fibrosis. Internal organ involvement is heterogeneous. It is unknown whether disease mechanisms are common across all involved affected tissues or if each manifestation has a distinct underlying pathology.We used consensus clustering to compare gene expression profiles of biopsies from four SSc-affected tissues (skin, lung, esophagus, and peripheral blood) from patients with SSc, and the related conditions pulmonary fibrosis (PF) and pulmonary arterial hypertension, and derived a consensus disease-associate signature across all tissues. We used this signature to query tissue-specific functional genomic networks. We performed novel network analyses to contrast …

Spectral Signatures Of Immature Lucilia Sericata (Meigen) (Diptera: Calliphoridae), Jodie-A. Warren, T. D. Pulindu Ratnasekera, David A. Campbell, Gail S. Anderson

Faculty Research, Scholarly, and Creative Activity

Hyperspectral remote sensing is an innovative technology with applications in many sciences and is a non-destructive method that may offer more precise aging within development stages. Hyperspectral reflectance measurements from the anterior, midsection, and posterior of Lucilia sericata (Meigen) larvae and pupae were conducted daily from samples of the developing insects beginning at second instar. Only midsection measurements were conducted on second instar larvae due to their size, to ensure that the measurement was not of reflective surroundings. Once measured, all insects were washed with deionized water, blotted with filter paper, and re-measured. Daily age prediction during the post-feeding stage …

Development Of Tools For Stable Transfection In The Human Filarial Parasite Brugia Malayi Via The Piggybac Transposon System, Johan Chabanon

USF Tampa Graduate Theses and Dissertations

Brugia malayi is one of three species of nematode known to cause lymphatic filariasis (LF) in humans. LF infects over 120 million people, causing debilitating disease. Various global programs have been launched in the past 20 years to eliminate LF. These programs have greatly scaled up the resources and efforts allocated to halting the transmission and reducing disease burden. Only a few drugs are used to treat LF, and resistance is thus a devastating possibility. Research aimed at identifying new drug targets could therefore prove essential in elimination of LF.

Genetic manipulation of B. malayi has been limited to transient …

Taxonomy, Ecology, And Behavior Of The Kleptoplastic Sea Slug Elysia Papillosa, William Alan Gowacki

USF Tampa Graduate Theses and Dissertations

Sacoglossan sea slugs are one of the best known examples of specialist herbivores in the marine environment and can be found strongly associated with their algal hosts and food sources. Perhaps the most intriguing characteristic of many sacoglossans is their ability to sequester functional chloroplasts from their algal food sources in a process called kleptoplasty. Despite this, there continues to be issues regarding taxonomic identification of species. In turn, the ecological characteristics of many of these slugs, such as algal host and food source preference, as well as their behavioral aspects, have received little attention. A prime example of these …

Identifying The Upstream Target Of Resveratrol In Breast Cancer Cells, Jeffrey Mecham, Jason Kenealey

Journal of Undergraduate Research

One commonly disrupted pathway in many different cancer cells lines revolves around the tumor suppressor protein _p53. Approximately half of human cancers exhibit direct, inactivating mutations of _p53, while the majority of the remainder either elevate inhibitors, reduce activators, or deactivate downstream targets of _p53¹. The central role of _p53 in preventing tumorigenesis stems from its ability to induce controlled cell death of unhealthy cells, along with DNA repair and cell cycle arrest.

Trafficking Receptor Signatures Define Blood Plasmablasts Responding To Tissue-Specific Immune Challenge, Yekyung Seong, Nicole Lazarus, Lusijah Sutherland, Aida Habtezion, Tzvia Abramson, Xiao-Song He, Harry Greenberg, Eugene Butcher

Faculty Publications, Biological Sciences

Antibody-secreting cells are generated in regional lymphoid tissues and traffic as plasmablasts (PBs) via lymph and blood to target sites for local immunity. We used multiparameter flow cytometry to define PB trafficking programs (TPs, combinations of adhesion molecules and chemoattractant receptors) and their imprinting in patients in response to localized infection or immune insults. TPs enriched after infection or autoimmune inflammation of mucosae correlate with sites of immune response or symptoms, with different TPs imprinted during small intestinal, colon, throat, and upper respiratory immune challenge. PBs induced after intramuscular or intradermal influenza vaccination, including flu-specific antibody–secreting cells, display TPs characterized …

Hierarchical Corannulene-Based Materials: Energy Transfer And Solid-State Photophysics, Allison M. Rice, W. Brett Fellows, Ekaterina A. Dolgopolova, Andrew B. Greytak, Aaron K. Vannucci, Mark D. Smith, Stavros G. Karakalos, Jeanette A. Krause, Stanislav M. Avdoshenko, Alexey A. Popov, Natalia B. Shustova Prof. Dr.

Faculty Publications

We report the first example of a donor–acceptor corannulene-containing hybrid material with rapid ligand-to-ligand energy transfer (ET). Additionally, we provide the first time-resolved photoluminescence (PL) data for any corannulene-based compounds in the solid state. Comprehensive analysis of PL data in combination with theoretical calculations of donor–acceptor exciton coupling was employed to estimate ET rate and efficiency in the prepared material. The ligand-to-ligand ET rate calculated using two models is comparable with that observed in fullerene-containing materials, which are generally considered for molecular electronics development. Thus, the presented studies not only demonstrate the possibility of merging the intrinsic properties of π-bowls, …

The Effects Of Trx Suspension Training On An Asymmetrical Weight Shift During A Double-Leg Squat, Samantha J. Shruck

Theses and Dissertations

Context: It is estimated that 54% of all sport related injuries are to the lower extremity, specifically the knee and ankle. Following injury, many individuals demonstrate an asymmetrical weight shift during a double-leg squat, which may increase risk of further injury. Asymmetries are driven by a variety of factors including range of motion and neuromuscular control. The goal of rehabilitation is to identify asymmetrical movement and utilize evidence-based techniques to retrain deficient movement patterns. The double-leg squat is a reliable movement task to identify asymmetrical weight shifts. Based on the theory and purpose of suspension training (TRX), it may be …

Fibroblast Proliferation In Response To Treatment By A Novel Tgf-Beta Pathway Inhibitor, Coray Preece, Robert Hyldahl

Journal of Undergraduate Research

Fibroblasts play a key role in repairing injured tissue by secreting collagen and growth factors into the tissue. In states of disease and overuse, fibroblast activity (i.e. greater fibroblast content and collagen secreting activity) can lead to skeletal muscle fibrosis, in which myofibers are replaced by collagen, decreasing strength and muscle elasticity. It has been shown in previous studies that TGF-beta signaling directly increases fibroblast proliferation and collagen secretion. It has also been shown that inhibition of TGF-beta signaling reduces known symptoms of muscular dystrophies including decreased fibrosis and increased muscular strength. SGI-1252 is a novel small molecule that our …

Interview With Eileen Gregory, Eileen Gregory, Wenxian Zhang

Oral Histories

Eileen Gregory received her BS from Michigan State University, an MS & PhD in microbiology from The University of Washington, where her research focused on freshwater bacteria. As a young professor in Biology, Eileen Gregory came to Rollins College in 1979, one of two women in the department’s faculty of five, when the sciences were dominated by men. She never expected to be at Rollins all her professional career: “I came to Rollins with the idea that I would stay here for three years and move on,” Gregory says. “But I love my colleagues; I love my students; and for …

Playing-With The World: Toy Story's Aesthetics And Metaphysics Of Play, Jonathan Hendricks

USF Tampa Graduate Theses and Dissertations

Pixar’s Toy Story (John Lassiter, 1995) is not just a story about toys and the children that play with them, but a demonstration of how we interact with the world. This thesis looks at the way in which both main children, Andy and Sid, interact with their toys and how this interaction is one that is structured by way of what Martin Heidegger calls “Enframing.” In this modality of playing, toys and other things and entities in the world, and the world itself, appear to the children as on-hand resources for use at any time and can be molded, as …

A Novel Abi-Domain Protein Controls Virulence Determinant Production In Staphylococcus Aureus, Stephanie Michelle Marroquin

USF Tampa Graduate Theses and Dissertations

A major factor in the success of Staphylococcus aureus as a pathogen is its vast arsenal of virulence determinants and, more importantly, the tight and precisely- timed regulation of these factors. Here we investigate the product of the S. aureus gene, SAUSA300_1984, encoding a putative transmembrane protein. This as yet uncharacterized protein belongs to the Abi (abortive infection) family, which are commonly annotated as CAAX-proteases, and are significantly understudied in prokaryotes. In S. aureus the disruption of SAUSA300_1984 results in a drastic reduction of proteolytic and hemolytic activity, as well as diminished pigmentation. This phenotype appears to be mediated through …

Patterns In Greater Sage-Grouse Population Dynamics Correspond With Public Grazing Records At Broad Scales, Adrian P. Monroe, Cameron L. Aldridge, Timothy J. Assal, Kari E. Veblen, David A. Pyke, Michael L. Casazza

Wildland Resources Faculty Publications

Human land use, such as livestock grazing, can have profound yet varied effects on wildlife interacting within common ecosystems, yet our understanding of land-use effects is often generalized from short-term, local studies that may not correspond with trends at broader scales. Here we used public land records to characterize livestock grazing across Wyoming, USA, and we used Greater Sage-grouse (Centrocercus urophasianus) as a model organism to evaluate responses to livestock management. With annual counts of male Sage-grouse from 743 leks (breeding display sites) during 2004-2014, we modeled population trends in response to grazing level (represented by a relative grazing index) …

Mass Action In Two-Sex Population Models: Encounters, Mating Encounters And The Associated Numerical Correction, Katherine Snyder, Brynja R. Kohler, Luis F. Gordillo

Mathematics and Statistics Faculty Publications

Ideal gas models are a paradigm used in Biology for the phenomenological modelling of encounters between individuals of different types. These models have been used to approximate encounter rates given densities, velocities and distance within which an encounter certainly occurs. When using mass action in two-sex populations, however, it is necessary to recognize the difference between encounters and mating encounters. While the former refers in general to the (possibly simultaneous) collisions between particles, the latter represents pair formation that will produce offspring. The classical formulation of the law of mass action does not account this difference. In this short paper, …