Life Sciences Commons^™

A Innovative Design Of Washer Machine For Thread Port Food Bottle Based On Triz Theory, Zhang Dan, Chang Hong, Lougao Xiang, Chang Peng

Food and Machinery

The bottle cleaning as a basis for the subsequent steps necessary at present, the bottle washer machine on the market ignored the bottle mouth and bottle cleaning thread, while the thread is the most dirty part of the bottle. Using TRIZ theory, analysis revealed summed up the problems in the design process, using innovative principle, field research, combined with the actual needs, set up product innovation design, rendering the physical map, get the user experience results. A Washer Machine model is established for the thread bottle, which solves the defect that the traditional bottle washer machine cannot effectively clean the …

Study On Components Of Essential Oil Of Mango Peel Extracted By Different Methods, Liang Xiumei, Hu Zhuoyan, Zhao Lei, Wang Kai

Food and Machinery

Steam distillation (SD), solvent extraction (SE) and supercritical CO₂ extraction (SFE-CO₂) were individually used to extract the essential oil in mango peel. The components and contents of essential oil were analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 80 compounds were identified in essential oils obtained by the three methods, including 22 terpenes, 8 hydrocarbons, 19 alcohols, 8 carboxides, 14 esters and 9 acids. The three methods detected 49 (SD), 56 (SE) and 59 (SFE-CO₂) compounds respectively. Comparison of the three methods showed, SD suit for the extraction of low boiling point and volatile …

A Research Of Intelligent Method For Evolving Blends Of Coffee, Guo Guangsong, Chen Liangji

Food and Machinery

In order to improve the quality and efficiency of coffee blend, a method based on interactive genetic algorithm (IGA) for it was studied. The blending solutions were used as individuals, with the improved adaptive crossover and mutation probability, and the interactive interface provides 10 indexes for individual fitness, and then five users participated the optimization. The results showed that the method was easy to operate, a consistent satisfactory solution could be obtained within 4～5 generations, with the difference of component ratio less than 5% and the fitness more than 85. Compared with the traditional cup testing, the results showed that …

Loss Of The Rna Helicase Skiv2l2 Impairs Mitotic Progression And Replication-Dependent Histone Mrna Turnover In Murine Cell Lines, Alexis Marie Onderak, James T. Anderson

Biological Sciences Faculty Research and Publications

RNA surveillance via the nuclear exosome requires cofactors such as the helicase SKIV2L2 to process and degrade certain noncoding RNAs. This research aimed to characterize the phenotype associated with RNAi knockdown of Skiv2l2 in two murine cancer cell lines: Neuro2A and P19. SKIV2L2 depletion in Neuro2A and P19 cells induced changes in gene expression indicative of cell differentiation and reduced cellular proliferation by 30%. Propidium iodide-based cell-cycle analysis of Skiv2l2 knockdown cells revealed defective progression through the G2/M phase and an accumulation of mitotic cells, suggesting SKIV2L2 contributes to mitotic progression. Since SKIV2L2 targets RNAs to the nuclear exosome for …

Oral History Conversation With Lydell Aaron (Beartha), Alexander Z. Bulkin, Kari Schattinger, Kate L. Gardner, Charbel Mouannes, Danielle Silvaggio

Philosophy: All Student Work

Oral History Interview conducted with Lydell Aaron. He founded Beartha Systems which builds scalable and smart systems that harness energy and resources for small communities.

Can Manure Management Be A Win-Win, Paul C. Hay

University of Nebraska-Lincoln Extension: Historical Materials

Manure and Soil Health: What is the State of the Science? Can manure be both an economic ‘Win’ and an environmental ‘Win’? What fields provide the best opportunity for Win/Win? What exactly are the environmental and soil health wins associated with manure? Can we test soils so that we have more understanding of the value of manure nutrients and carbon? What are the biological processes in a soil system that benefit from organic fertilizers? These and other questions are the topics a new University of Nebraska working group addressing Manure and Soil Health (MaSH) plan to address. Rick Koelsch, University …

Inflammatory Role Of High Salt Level In Tumor Microenvironment (Review), Suneetha Amara, Venkataswarup Tiriveedhi

Biology Faculty Research

Chronic inflammation is known to play a critical role in cancer development and progression. High salt is known to mediate several chronic inflammatory diseases including hypertension, myocardial infarction, neurological ischemic attack, autoimmune diseases and cancers. High salt level is shown to induce angiogenesis and immune-dysfunction, both of which play a direct role in cancer proliferation. Furthermore, salt has been suggested to enhance Warburg-like metabolic phenotype in cancer cells and at the same time also induce pro-tumor MΦ2-macrophage phenotype. Recent studies have identified several molecular targets such as tonicity specific transcript factor NFAT5/TonEBP, sodium ion channel γENaC, and vascular endothelial growth …

Justo Sierra Cruise Sample Data, Southern Gulf Of Mexico, 2015, Patrick Schwing

C-IMAGE data

This dataset includes CTD data from two cruises aboard the B/O Justo Sierra in 2015. Data are in ACSII and RAW format.

The Mediating Effect Of Self-Determined Motivation In Student-Athlete Perceptions Of Coaching Behaviors And Its Effect On Grit And Mental Toughness, Lloyd Scharneck

Theses and Dissertations

The aim of this current study was to examine the relationship between coaching behaviors, grit, mental toughness, and motivation. I hypothesized that coaching behaviors would have an indirect influence on grit and mental toughness through the motivational orientations of athletes. To examine these relationships, we surveyed 219 student athletes from Midwestern universities using valid and reliable measures assessing the key constructs in this study. Results revealed significant positive relationships between autonomy-supportive coaching, grit, mental toughness and autonomous motivation. Significant negative relationships between controlling coach, grit, and mental toughness were also found. Path analysis revealed two major findings. A significant positive …

Hearing On The Fly: The Effects Of Wing Position On Noctuid Moth Hearing, Shira D. Gordon, Elizabeth Klenschi, James F. C. Windmill

Dartmouth Scholarship

The ear of the noctuid moth has only two auditory neurons, A1 and A2, which function in detecting predatory bats. However, the noctuid's ears are located on the thorax behind the wings. Therefore, as these moths need to hear during flight, it was hypothesized that wing position may affect their hearing. The wing was fixed in three different positions: up, flat and down. An additional subset of animals was measured with freely moving wings. In order to negate any possible acoustic shadowing or diffractive effects, all wings were snipped, leaving the proximal-most portion and the wing hinge intact. Results revealed …

Neutralizing Anti-Interleukin-1Β Antibodies Reduce Ischemia-Related Interleukin-1Β Transport Across The Blood-Brain Barrier In Fetal Sheep, Aparna Patra, Xiaodi Chen, Grazyna B. Sadowska, Jiyong Zhang, Yow-Pin Lim, James F. Padbury, William A. Banks, Barbara S. Stonestreet

Pediatrics Faculty Publications

Hypoxic ischemic insults predispose to perinatal brain injury. Pro-inflammatory cytokines are important in the evolution of this injury. Interleukin-1β (IL-1β) is a key mediator of inflammatory responses and elevated IL-1β levels in brain correlate with adverse neurodevelopmental outcomes after brain injury. Impaired blood-brain barrier (BBB) function represents an important component of hypoxic-ischemic brain injury in the fetus. In addition, ischemia-reperfusion increases cytokine transport across the BBB of the ovine fetus. Reducing pro-inflammatory cytokine entry into brain could represent a novel approach to attenuate ischemia-related brain injury. We hypothesized that infusions of neutralizing IL-1β monoclonal antibody (mAb) reduce IL-1β transport across …

A Critical Analysis Of The Direct Effects Of Dredging On Fish, Amelia S. Wenger, Euan S. Harvey, Shaun K. Wilson, Christopher A. Rawson, Stephen J. Newman, Douglas Clarke, Benjamin J. Saunders, Nicola Browne, Michael J. Travers, Jennifer L. Mcilwain, Paul L A Erftemeijer, Jean-Paul A. Hobbs, Dianne L. Mclean, Martial Depczynski, Richard D. Evans

Fisheries Research Articles

Dredging can have significant impacts on aquatic environments, but the direct effects on fish have not been critically evaluated. Here, a meta-analysis following a conservative approach is used to understand how dredging-related stressors, including suspended sediment, contaminated sediment, hydraulic entrainment and underwater noise, directly influence the effect size and the response elicited in fish across all aquatic ecosystems and all life-history stages. This is followed by an in-depth review summarizing the effects of each dredging-related stressor on fish. Across all dredging-related stressors, studies that reported fish mortality had significantly higher effect sizes than those that describe physiological responses, although indicators …

Cooperation And Competition Shape Ecological Resistance During Periodic Spatial Disturbance Of Engineered Bacteria, Cortney E. Wilson, Allison J. Lopatkin, Travis J. A. Craddock, William W. Driscoll, Omar T. Eldakar, Jose V. Lopez, Robert P. Smith

Biology Faculty Articles

Cooperation is fundamental to the survival of many bacterial species. Previous studies have shown that spatial structure can both promote and suppress cooperation. Most environments where bacteria are found are periodically disturbed, which can affect the spatial structure of the population. Despite the important role that spatial disturbances play in maintaining ecological relationships, it remains unclear as to how periodic spatial disturbances affect bacteria dependent on cooperation for survival. Here, we use bacteria engineered with a strong Allee effect to investigate how the frequency of periodic spatial disturbances affects cooperation. We show that at intermediate frequencies of spatial disturbance, the …

Plp And Gaba Trigger Gabr-Mediated Transcription Regulation In Bacillus Subtilis Via External Aldimine Formation, Rui Wu, Ruslan Sanishvili, Boris R. Belitsky, Jose I. Juncosa, Hoang V. Le, Helaina J. S. Lehrer, Michael Farley, Richard B. Silverman, Gregory A. Petsko, Dagmar Ringe, Dali Liu

Chemistry: Faculty Publications and Other Works

The Bacillus subtilis protein regulator of the gabTD operon and its own gene (GabR) is a transcriptional activator that regulates transcription of γ-aminobutyric acid aminotransferase (GABA-AT; GabT) upon interactions with pyridoxal-5′-phosphate (PLP) and GABA, and thereby promotes the biosynthesis of glutamate from GABA. We show here that the external aldimine formed between PLP and GABA is apparently responsible for triggering the GabR-mediated transcription activation. Details of the “active site” in the structure of the GabR effector-binding/oligomerization (Eb/O) domain suggest that binding a monocarboxylic γ-amino acid such as GABA should be preferred over dicarboxylic acid ligands. A reactive GABA analog, ( …

Mass-Spectrometry Based Proteomics Of Age-Related Changes In Murine Microglia, Antwoine Flowers

USF Tampa Graduate Theses and Dissertations

The last century has seen a steady increase in the extension of the average lifespan. This has concomitantly produced higher incidences of age-related chronic degenerative diseases like Alzheimer’s and Parkinson’s diseases. Age is the single greatest risk factor for the development of not just these degenerative conditions but cancer as well. The aged niche undergoes a number of maladaptive changes that allow underlying conditions to present and progress. Exactly which changes, contribute to the progression of which disease is currently an area of intense study. However, these answers often present therapeutic targets for disease prevention. Age is characterized by a …

Comparison Of Clinician Assisted Versus Unassisted Pnf Stretching On Hamstring Flexibility, Isometric Force Output, And Maximum Vertical Jump Height, Joel T. Lockhart

Theses and Dissertations

Introduction: Hamstring strains are among one of the most common acute injuries that occur in soccer players. Previous research has shown that reduced hamstring flexibility is a risk factor for injury, however this is modifiable through stretching. Some stretching techniques have contributed to decreased performance on measures important to success in sport. Proprioceptive neuromuscular facilitation (PNF) is one effective stretching technique to improve hamstring flexibility, but requires a trained partner or clinician to perform properly. It is unknown if the performance of PNF stretching without a clinician’s assistance could be as effective. It is also unknown if PNF stretching affects …

Minor In Proficiency In Ocean Data Science (Pods), Joanna Burkhardt

Library Impact Statements

No abstract provided.

A Prototypical First-Generation Electronic Cigarette Does Not Reduce Reports Of Tobacco Urges Or Withdrawal Symptoms Among Cigarette Smokers, Arit M. Harvanko, Catherine A. Martin, Richard J. Kryscio, William W. Stoops, Joshua A. Lile, Thomas H. Kelly

Psychology Faculty Publications

It is unknown whether first-generation electronic cigarettes reduce smoking urges and withdrawal symptoms following a 24 h deprivation period. This study tested whether a first-generation electronic cigarette reduces smoking urges and withdrawal symptoms in cigarette smokers. Following 24 h of tobacco deprivation, using a within-subjects design, eight nontreatment seeking tobacco cigarette smokers (3 females) administered 10 puffs from a conventional cigarette or a first-generation electronic cigarette containing liquid with 0, 8 or 16 mg/ml nicotine. Conventional cigarettes ameliorated smoking urges and electronic cigarettes did not, regardless of nicotine concentration. First-generation electronic cigarettes may not effectively substitute for conventional cigarettes in …

Smokejumper Obituary: Trankle, Hans W. (Missoula 1951), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

47th Annual Wku Student Research Conference, Student Research Council,

Student Research Conference Select Presentations

See document below for project abstracts

Comparison Of Leaf Water Potential, Stomatal Conductance, And Chlorophyll Fluorescence Between An Invasive Weed, Schinus Molle, And A Native Chaparral Shrub, Ceanothus Spinosus, In The Santa Monica Mountains, Adam L. Fahey, Harry Zhou, Katie Callahan

Seaver College Research And Scholarly Achievement Symposium

The Peruvian peppertree, Schinus molle, is an invasive species in California that has escaped cultivation to competitively exclude native species in the landscape. One example of such competition is between a dominant member of the chaparral shrub community, greenbark ceanothus (Ceanothus spinosus), and S. molle. The mechanism of competitive displacement of chaparral by S. molle is not understood nor has it been previously studied. We hypothesized that during severe drought, such as occurred during the sumer/fall of 2016, S. molle would have a more favorable water balance then its native competitor C. spinosus, eventually leading to higher survival and species …

Effect Of Drought On Resin Production In Woody Stems Of Rhus Ovata, Sydney T. Adams, Melody Young, Moriah Hall

Seaver College Research And Scholarly Achievement Symposium

Resin production in stems of woody plants is generated from resin canal secretions and is thought to play a significant role in protection from fungal infection and spread. This may be one of several factors associated with fungal infection and fungal induced dieback in plants during severe water stress or drought. We set out to determine if reduction in resin production in a chaparral shrub, Sugar Bush (Rhus ovata), was associated with severe drought in southern California. We hypothesized that dehydrated R. ovata would produce less resin than hydrated plants. Our null hypothesis was that there is no …

Genetic Effect Of Drought On The California Newt, Taricha Torosa, Madeleine M. Perrault, Samantha Fiallo, Alero Arueyingho, Cindy Kim, Laiken Ritchie

Seaver College Research And Scholarly Achievement Symposium

Droughts are now a common occurrence in Southern California and are known to cause population declines in many species, and such bottlenecks can cause a loss of genetic variation. Genetic variation was examined in a population of the California newt located in Arroyo Sequit Creek in the Santa Monica Mountains. Genetic data from animals examined prior to the current drought were compared to similar data collected from the population after it experienced several years of severe drought. We hypothesized that the drought impacted population should show less genetic variation in comparison to the pre-drought population. Nevertheless, there were no genetic …

Hydraulic Mechanisms Of Fungal-Induced Dieback In A Keystone Chaparral Species During Unprecedented Drought In California, Natalie M. Aguirre, Marissa E. Ochoa, Helen I. Holmlund, Frank E. Ewers, Stephen D. Davis

Seaver College Research And Scholarly Achievement Symposium

Between 2012-2016, southern California experienced unprecedented drought that caused dieback in Malosma laurina, a keystone species of chaparral shrub communities. Dieback was especially severe in coastal exposures of the Santa Monica Mountains, leading to whole plant mortality exceeding 50% at some sites. We hypothesized that the endophytic fungus causing the dieback, Botryosphaeria dothidea, was successful in invading the xylem tissue of M. laurina because of protracted water stress, carbon starvation, or a combination of the two. We tested these possibilities in a controlled pot experiment by comparing three treatments, each inoculated with the fungus: (1) irrigated controls (2) …

Plant Water Relations During Drought-Induced Dieback In Greenbark Ceanothus, Ceanothus Spinosus, In The Santa Monica Mountains, Christopher F. Ross, Andrew Palaski

Seaver College Research And Scholarly Achievement Symposium

This experiment investigated Ceanothus spinosus dieback in the natural landscape of Pepperdine University’s campus in Malibu, California. We measured midday water potential, gas-exchange rates (photosynthesis and transpiration), chlorophyll fluorescence, and native embolism levels of stem xylem, comparing irrigated controls on campus to plants undergoing severe water stress in the natural landscape of our campus. The water potentials for naturally occurring C. spinosus (-6.6 MPa) was much lower than for irrigated controls (-3.4 MPa). Water stressed plants experience 47.2% embolism in their stem xylem that blocks water transport to leaves compared to 12.6% embolism in irrigated controls. This high embolism level …

A Comparison Of Water Potential, Photosynthetic Rate, Electron Transport Rate, And Stomatal Conductance Between Native Malosma Laurina And Exotic Schinus Molle, Karagan L. Smith, Agatha C. Heng, Viridiana Hernandez-Lopez

Seaver College Research And Scholarly Achievement Symposium

A Comparison of Water Potential, Photosynthetic Rate, Electron Transport Rate, and Stomatal Conductance between Native Malosma laurina and Exotic Schinus molle

Karagan L. Smith, Agatha C. Heng, and Viridiana Hernandez-Lopez

First-year Students as Scholars Program

Mentors: Natalie M. Aguirre, Kaitlyn E. Sauer, and Stephen D. Davis

Schinus molle is a relatively new invasive species in chaparral shrub communities of the Santa Monica Mountains, and has only recently been observed to displace native species of shrubs, such as Malosma laurina. To investigate the probably cause of S. molle’s invasiveness and mechanisms of competitive displacement of M. laurina, we compared …

Progression Of Activity And Predator Avoidance In California Grunion Larvae, Callyn E. Shelley

Seaver College Research And Scholarly Achievement Symposium

No abstract provided.

Carbon Black Exposure Induces Alterations In Mitochondrial Morphology In Human Lung Cells: A Software-Based Quantitative Analysis, Jennifer D. Duff, Brianna K. Laferney, Celina Y. Jones, Penn M. Mcclatchey, Jay Brewster

Seaver College Research And Scholarly Achievement Symposium

Nanoparticulates of pure carbon, carbon black (CB), are a common atmospheric pollutant in industrialized and heavily populated areas. They are produced primarily via combustion of fossil fuels, and represent a significant health hazard. They are known to worsen asthma and bronchitis when inhaled and to cause inflammation, heart dysfunction, and oxidative stress when incorporated into other organs. The key focal point of this work was to examine markers of stress signaling and cellular dysfunction when human bronchial epithelial cells (16HBE14o-) were exposed to CB particles ranging in size from 70 nm and averaging 130 nm in diameter. BrdU incorporation and …

The Effect Of Water Levels On Crayfish Aggression, Emory B. Johnson, Sarah E. Blase

Seaver College Research And Scholarly Achievement Symposium

Crayfish are a very prevalent invasive species that affect many different environments all over the world. Crayfish, specifically Procambarus clarkii, are an invasive species that have currently taken a foothold in the Santa Monica mountains and are threatening native stream species. There is currently a severe drought occurring in the Santa Monica mountains. The drought has been reshaping many aquatic environments by limiting space and resources such as food and shelter. Crayfish are a very aggressive species and adversely affect the habitat they inhabit by rapidly colonizing, burrowing, and their voracious diets. Due to the adverse effects crayfish can have …

The North American Whistling-Ducks, Pochards, And Stifftails, Paul A. Johnsgard

Zea E-Books Collection

Although the 12 species representing three waterfowl tribes described in this volume are not closely related, they fortuitously provide an instructive example of adaptive evolutionary radiation within the much larger waterfowl lineage (the family Anatidae), especially as to their divergent morphologies, life histories, and social behaviors.

The whistling-ducks (Dendrocygna), with three known North American species, are notable for their permanent pair-bonds, extended biparental family care, and strong social cohesion. In contrast, males of the five typical pochards of North American diving ducks (Aythya) establish monogamous pair-bonds that are maintained only long enough to assure that the female’s eggs are fertilized. …