Biomedical Commons^™

Graph Information Processing For Artificial Intelligence, Limeng Pu

LSU Doctoral Dissertations

In the last decade, techniques for artificial intelligence (AI) has advanced tremendously, which lead to solutions to many problems that have long-troubled us. Such examples include image/video recognition, speech recognition, and 3D scenario recognition. As the tool become more and more powerful, we started to explore data types that have never been handled in an AI fashion. Graph is undoubtedly the first one that comes to mind. Many important real-life data is or can be represented as graphs or networks: social networks, communication networks, protein-protein interaction networks, molecular structures, etc. Yet very little attention has been devoted to the study …

Effects Of Pulse Width On He Plasma Jets In Contact With Water Evaluated By Oh(A-X) Emission And Ohaq Production, Shutong Song, Esin B. Sözer, Chunqi Jiang

Bioelectrics Publications

Nanosecond pulsed helium plasma jets impinging on water produce hydroxyl radicals both in gas- and liquid-phase. In this study, the effects of pulse width on a repetitively pulsed plasma jet in contact with water are evaluated via OH(A-X) emission and OH_aq production in water for various pulse widths ranging from 200 to 5000 ns. The maximal energy efficiency of OH(A-X) emission is obtained for pulse widths of 600-800 ns whereas the maximal efficiency of OH_aq production is at 200 ns. Temporally-resolved emission spectroscopy shows that more than 40% of OH(A-X) emission is produced during the first 200 ns …

Portable Electrocardiogram Device And Signal Processing Design, Ryan F. Blaalid

Electrical Engineering

Full 12-lead electrocardiogram (ECG) measurements require inconvenient and time consuming adhesive electrode placement. This project proposes a design for a Bluetooth based ECG for remote patient measurement. The device is designed to measure up to 6-leads and utilizes 5 dry (non-adhesive) electrodes to accomplish this. The device delivers the ECG data to the user’s mobile smart phone and can then be sent to the patient’s doctor for analysis. Since the contact of the dry electrodes to the skin is not perfect, low-frequency noise called baseline wandering is introduced. A signal processing technique borrowed from image processing called morphological filtering is …

Eeg Classification Using Residual Neural Networks, Eren Esener

Electrical Engineering

There is a growing desire to understand the EEG (electroencephalogram) signals related with brain activities. In order to analyze EEG signals, they first must be measured by sensors, which induces a lot of noise; then these signals are classified to understand the intended actions. In many cases a neural network is used as the algorithms for classification. A residual neural network (ResNet) is an artificial neural network (ANN) of a kind that are related with the pyramidal cells in the cerebral cortex. The goal of this work is to investigate the viability of ResNets for classifying EEG signals for hand …

Surface-Enhanced Raman Detection Of Glucose On Several Novel Substrates For Biosensing Applications, Laila Saad Alqarni

Dissertations

The small normal Raman cross-section of glucose is considered to be a major challenge for its detection by Surface Enhanced Raman Spectroscopy (SERS) for medical applications. These applications include blood glucose level monitoring of diabetic patients and evaluation of patients with other medical conditions, since glucose is a marker for many human diseases. This dissertation focuses on Surface-Enhanced Raman Scattering primarily for the detection of glucose. Some experiments also are carried out for the detection of the corresponding enzyme glucose oxidase that is used in electrochemical glucose sensors and in biofuel cells. This project explores the possibility of utilizing Surface …

Developing An Optomechanical Approach For Characterizing Mechanical Properties Of Single Adherent Cells, Ali Mehrnezhad

LSU Doctoral Dissertations

Mechanical properties of a cell reflect its biological and pathological conditions including cellular disorders and fundamental cellular processes such as cell division and differentiation. There have been active research efforts to develop high-throughput platforms to mechanically characterize single cells. Yet, many of these research efforts are focused on suspended cells and use a flow-through configuration. Therefore, adherent cells are detached prior to the characterization, which seriously perturbs the cellular conditions. Also, methods for adherent cells are limited in their throughput.

My study is aimed to fill the technical gap in the field of single cell analysis, which is a high-throughput …

Development Of A Sonically Powered Biodegradable Nanogenerator For Bone Regeneration, Avi S. Patel

University Scholar Projects

Background: Reconstruction of bone fractures and defects remains a big challenge in orthopedic surgery. While regenerative engineering has advanced the field greatly using a combination of biomaterial scaffolds and stem cells, one matter of difficulty is inducing osteogenesis in these cells. Recent works have shown electricity’s ability to promote osteogenesis in stem cell lines when seeded in bone scaffolds; however, typical electrical stimulators are either (a) externally housed and require overcomplex percutaneous wires be connected to the implanted scaffold or (b) implanted non-degradable devices which contain toxic batteries and require invasive removal surgeries.

Objective: Here, we establish a biodegradable, piezoelectric …

Development Of A Sonically Powered Biodegradable Nanogenerator For Bone Regeneration, Avi Patel

Honors Scholar Theses

Background: Reconstruction of bone fractures and defects remains a big challenge in orthopedic surgery. While regenerative engineering has advanced the field greatly using a combination of biomaterial scaffolds and stem cells, one matter of difficulty is inducing osteogenesis in these cells. Recent works have shown electricity’s ability to promote osteogenesis in stem cell lines when seeded in bone scaffolds; however, typical electrical stimulators are either (a) externally housed and require overcomplex percutaneous wires be connected to the implanted scaffold or (b) implanted non-degradable devices which contain toxic batteries and require invasive removal surgeries.

Objective: Here, we establish a biodegradable, piezoelectric …

Bamorc: A Software Package For Accurate And Robust 13C Reference Correction Of Protein Nmr Spectra, Xi Chen, Andrey Smelter, Hunter N. B. Moseley

Molecular and Cellular Biochemistry Faculty Publications

We describe Bayesian Model Optimized Reference Correction (BaMORC), a software package that performs ¹³C chemical shifts reference correction for either assigned or unassigned peak lists derived from protein NMR spectra. BaMORC provides an intuitive command line interface that allows non-nuclear magnetic resonance (NMR) experts to detect and correct ¹³C chemical shift referencing errors of unassigned peak lists at the very beginning of NMR data analysis, further lowering the bar of expertise required for effective protein NMR analysis. Furthermore, BaMORC provides an application programming interface for integration into sophisticated protein NMR data analysis pipelines, both before and after the …

Left Atrial Model, Borna Sobati, Sarah Porello, Tess Pate

Biomedical Engineering

The objective is to produce an electrophysiological model of an adult human left atrium. This model will be used to test mapping probe catheters used for locating cardiac arrhythmias against current technology used in practice. Dr. Chris Porterfield requested this model and other physicians or probe catheter manufacturers may also use this product in the future. Dr. Porterfield also discussed the possibility of future senior project groups using the model as a bench test for designing new catheter tips. The model will precisely simulate electrical behaviors of the heart in normal as well as arrhythmic conditions. Ideally, the model will …

Automatic Signal And Image-Based Assessments Of Spinal Cord Injury And Treatments., Samineh Mesbah

Electronic Theses and Dissertations

Spinal cord injury (SCI) is one of the most common sources of motor disabilities in humans that often deeply impact the quality of life in individuals with severe and chronic SCI. In this dissertation, we have developed advanced engineering tools to address three distinct problems that researchers, clinicians and patients are facing in SCI research. Particularly, we have proposed a fully automated stochastic framework to quantify the effects of SCI on muscle size and adipose tissue distribution in skeletal muscles by volumetric segmentation of 3-D MRI scans in individuals with chronic SCI as well as non-disabled individuals. We also developed …

Segmentation And Registration Based Automatic Cancer Proton Treatment Analysis, Yang Zhang

Open Access Theses & Dissertations

Due to its low side effects, proton therapy is rapidly developing around the world. However, the time and labor it takes to deliver the treatment have prevented widespread use in the clinic setting. For this reason, an automatic proton treatment analysis system is needed to improve efficiency during the treatment process.

The challenge lies in improving the accuracy and speed of the current proton treatment analysis system. The Range shifter correction factor is considered the same value under any given condition in our treatment system. This approximate algorithm limits the accuracy of the proton treatment analysis system. In addition, medical …

The Effects Of Bone Morphogenic Protein 2 On The Viability And Proliferation Of Dental Pulp Stem Cell Isolates, Joseph Cinelli

UNLV Theses, Dissertations, Professional Papers, and Capstones

Growth factor is an umbrella term used to describe a signaling molecule present in organisms that serves the purpose of influencing a stimulatory or inhibitory response from the target cell it acts on. Growth factors are categorized and classified by the types of tissues they act on and the cellular responses they illicit. For example, there are growth factors that act primarily in connective tissue on fibroblasts and they are aptly classified as the fibroblast growth factor family. Similarly, there is another family of growth factors that act on bone forming cells, among others, and they are known as bone …

The Affective Perceptual Model: Enhancing Communication Quality For Persons With Pimd, Jadin Tredup

UNLV Theses, Dissertations, Professional Papers, and Capstones

Methods for prolonged compassionate care for persons with Profound Intellectual and Multiple Disabilities (PIMD) require a rotating cast of import people in the subjects life in order to facilitate interaction with the external environment. As subjects continue to age, dependency on these people increases with complexity of communications while the quality of communication decreases. It is theorized that a machine learning (ML) system could replicate the attuning process and replace these people to promote independence. This thesis extends this idea to develop a conceptual and formal model and system prototype.

The main contributions of this thesis are: (1) proposal of …

Three-Dimensional Plasma Cell Survival Microniche In Multiple Myeloma, Katrina A. Harmon

Theses and Dissertations

Multiple myeloma (MM) is an incurable malignancy characterized by the uncontrolled proliferation of long-lived plasma cells (PCs) in the bone marrow (BM), which constitute at least 10% of BM cellularity. Normally, long-lived plasma cells make up less than 1% of BM cells. Plasma cells become neoplastic when a clonal PC population produces a monoclonal immunoglobulin protein. A diagnosis of monoclonal gammopathy of undetermined significance (MGUS) is made when there is an increase in monoclonal PCs within the BM, but less than 10%, and the patient does not present with end-organ damage, which is associated with active MM. Though not considered …

Biophysical Analyses Of Left Ventricular Remodeling Secondary To Myocardial Infarction And Left Ventricular Pressure Overload, William Manuel Torres

Theses and Dissertations

Left ventricular (LV) remodeling is nominally an adaptive process that restores biomechanical function following myocardial injury and/or sustained alterations in loading conditions. This remodeling can materialize as changes in myocardial geometry, composition, and mechanical properties. When these changes fail to restore LV biomechanical function, remodeling is termed maladaptive. It is generally accepted that maladaptive LV remodeling underlies the progression to heart failure in various forms of heart disease. The central hypothesis of this study is that we can leverage echocardiographic imaging techniques to non-invasively quantify changes in biomechanical function and mechanical properties in a serial manner throughout the progression towards …

Three-Dimensional Plasma Cell Survival Microniche In Multiple Myeloma, Katrina A. Harmon

Theses and Dissertations

Multiple myeloma (MM) is an incurable malignancy characterized by the uncontrolled proliferation of long-lived plasma cells (PCs) in the bone marrow (BM), which constitute at least 10% of BM cellularity. Normally, long-lived plasma cells make up less than 1% of BM cells. Plasma cells become neoplastic when a clonal PC population produces a monoclonal immunoglobulin protein. A diagnosis of monoclonal gammopathy of undetermined significance (MGUS) is made when there is an increase in monoclonal PCs within the BM, but less than 10%, and the patient does not present with end-organ damage, which is associated with active MM. Though not considered …

Beneficial Effects Of Resveratrol Against Colitis And Colorectal Cancer Mediated By The Host Microbiome, Epigenome, And Immune Response, Haider Rasheed Daham Alrafas

Theses and Dissertations

Resveratrol, a natural polyphenol compound found in red wine, the skins of grapes, and other plant products, has been used as a traditional medicine for thousands of years throughout human history, but current research has revealed this natural component is capable of modulating a variety of immunological, microbial, and epigenetic mechanisms to improve overall health and well-being of hosts that consume it. Colitis, an inflammatory bowel disease characterized by chronic inflammation in the colon and rectum, which has been associated with colon cancer. This cancer incidence is rising in younger adults in the US. Thus, newer approaches to prevent colitis …

Wearable Sensor Network Of Inertial Measurement Units To Track, Classify, And Assess Performance Of Complex Body Motions, Richard Tuggle

Research Horizons Day Posters

No abstract provided.

An Electrooculography Prosthetic Control System, Walker Arce

UNO Student Research and Creative Activity Fair

For many surface EMG (sEMG) prosthetic control systems, the muscles on the affected limb are used to trigger the control system. This can lead to fatigue in the user as they have to maintain continuous muscle activation and coactivation in order to achieve prosthetic control [3]. The crux of any prosthetic control system is the human machine interface that is developed, and at present many control systems have complicated interfaces that limit the degrees of freedom of the prosthetics. This study focuses on the development of a low-cost prosthetic control peripheral that is compatible with the prosthetics developed by Dr. …

A Voice Activated Prosthetic Mode Of Control, Will Picken

UNO Student Research and Creative Activity Fair

There are many options for a prosthetic mode of control. The most commonly created modes of control involve surface EMG (sEMG) and require that the user have well defined muscle activation in order to trigger the movement of the prosthetic. Unfortunately, many prosthetic users do not have the required muscle tone or strength to trigger these sensors, so the need for a control mechanism that doesn’t rely on sEMG is clear. This study focuses on the development of a low-cost, voice activated prosthetic mode of control compatible with the prosthetics developed by Dr. Zuniga and his research team.

The printed …

A Radio Frequency Identification (Rfid) Prosthetic Control System, Andrew Butler

UNO Student Research and Creative Activity Fair

Item classification and tagging in embedded systems has become ubiquitous in our society due to radio frequency identification (RFID) and the cost has dropped significantly for hardware that can interact with these devices. Due to this, possibilities in the realm of prosthetic control systems opens up for dynamic grip selection without user intervention. This study focuses on the development of a device capable of this functionality that is compatible with the prosthetics designed by Dr. Zuniga and his research team.

The printed circuit board (PCB) was designed using the EAGLE EDA package and incorporates a lithium polymer battery with a …

Development Of A Myoelectric Detection Circuit Platform For Computer Interface Applications, Nickolas Andrew Butler

Master's Theses

Personal computers and portable electronics continue to rapidly advance and integrate into our lives as tools that facilitate efficient communication and interaction with the outside world. Now with a multitude of different devices available, personal computers are accessible to a wider audience than ever before. To continue to expand and reach new users, novel user interface technologies have been developed, such as touch input and gyroscopic motion, in which enhanced control fidelity can be achieved. For users with limited-to-no use of their hands, or for those who seek additional means to intuitively use and command a computer, novel sensory systems …

A Pulse Sensor Interface Design For Fpga Based Multisensor Health Monitoring Platform, Yu Wang, Sunghoon Jang

Publications and Research

The FPGA-based platform is critical for producing an inexpensive early validation platform design. In past years, sensor nodes based on the FPGA platform have been proposed to be IoT low-end devices. In this study, we present the FPGA based IoT low-end reconfigurable pulse sensor interface design that can be integrated with a multi-sensor healthcare platform to monitor a human pulse vital sign and be able to distinguish between user normal, Bradycardia, or Tachycardia heart rate. The pulse sensor interface is implemented by VHDL programming and FPGA technology. The designed pulse sensor peripheral interface is reliable and reconfigurable. It can collect …

Design And Optimization Of An Ionizing-Radiation-Free Dental Imaging Scheme By Near Infrared Fluorescence, Thomas Hartzler

LSU Master's Theses

There is a global need for access to oral health care. Proper oral health care is necessary for physiological and psychological health, as well as social and economic issues. Major oral diseases that must be screened include caries, cracked teeth, and impacted molars. These diseases are screened and diagnosed using a variety of medical imaging methods, computed tomography (CT) X-ray being the most popular and efficient method. The challenges in screening with X-ray technology include financial and time cost as well as health risk, including radiation exposure induced cancer (REIC). This thesis summarizes research to explore the possibility of overcoming …

Telemedicine: An Iot Application For Healthcare Systems, Mohammad Abdellatif, Walaa Mohamed

Electrical Engineering

Telemedicine is the abstract term used to define medical services delivered through Information Technology and Telecommunications. With the growing advancements in the field of data mining, pattern recognition, expert systems, and image processing, the motivation of such a technology has increased. This allowed Telemedicine to be a stable solution especially because people are starting to trust the system more for its higher accuracy. This paper proposes a Telemedicine platform between the patient and the doctor. This platform belongs to the internet of medical things (IoMT) by enabling multiple medical sensors to connect to a server either using Wi-Fi, Bluetooth or …

Mechanisms And Immunogenicity Of Nspef-Induced Cell Death In B16f10 Melanoma Tumors, Alessandra Rossi, Olga N. Pakhomova, Andrei G. Pakhomov, Samantha Weygandt, Anna A. Bulysheva, Len E. Murray, Peter A. Mollica, Claudia Muratori

Bioelectrics Publications

Accumulating data indicates that some cancer treatments can restore anticancer immunosurveillance through the induction of tumor immunogenic cell death (ICD). Nanosecond pulsed electric fields (nsPEF) have been shown to efficiently ablate melanoma tumors. In this study we investigated the mechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors. Our data show that in vitro nsPEF (20-200, 200-ns pulses, 7 kV/cm, 2 Hz) caused a rapid dose-dependent cell death which was not accompanied by caspase activation or PARP cleavage. The lack of nsPEF-induced apoptosis was confirmed in vivo in B16F10 tumors. NsPEF also failed to trigger ICD-linked responses such …

Quantifying The Concentration And Penetration Depth Of Long-Lived Rons In Plasma Activated Water By Uv Absorption Spectroscopy, Zhijie Liu, Chunxi Zhou, Dingxin Liu, Tongtong He, Li Guo, Dehui Xu, Michael G. Kong

Bioelectrics Publications

Reactive oxygen and reactive nitrogen species (RONS) are believed to play a key role in biomedical applications, which means that RONS must reach the target tissue to produce a therapeutic effect. Existing methods (electron spin spectrometry and microplate reading) to determine the RONS concentration are not suitable for experimental real-time measurements because they require adding an indicating reagent to the plasma-treated medium, which may alter the chemical composition of the medium. In this paper, we propose a method to measure the long-lived RONS concentration in plasma-activated water (PAW) by using UV absorption spectroscopy. Based on an analysis and fit of …

Nanosecond Pulsed Electric Fields Induce Endoplasmic Reticulum Stress Accompanied By Immunogenic Cell Death In Murine Models Of Lymphoma And Colorectal Cancer, Alessandra Rossi, Olga N. Pakhomova, Peter A. Mollica, Maura Casciola, Uma Mangalanathan, Andrei G. Pakhomov, Claudia Muratori

Bioelectrics Publications

Depending on the initiating stimulus, cancer cell death can be immunogenic or non-immunogenic. Inducers of immunogenic cell death (ICD) rely on endoplasmic reticulum (ER) stress for the trafficking of danger signals such as calreticulin (CRT) and ATP. We found that nanosecond pulsed electric fields (nsPEF), an emerging new modality for tumor ablation, cause the activation of the ER-resident stress sensor PERK in both CT-26 colon carcinoma and EL-4 lymphoma cells. PERK activation correlates with sustained CRT exposure on the cell plasma membrane and apoptosis induction in both nsPEF-treated cell lines. Our results show that, in CT-26 cells, the activity of …

Synthesis, Characterization And Antibacterial Properties Of Silver(I) N-(4-Pyridyl) Benzamide Complexes With Varying Conterions, Mauro J. Ruiz

Open Access Theses & Dissertations

Organic semiconductor materials composed of - stacking aromatic compounds have been under intense investigation for their potential uses in flexible electronics and other advanced technologies. Herein we report a new family of seven - stacking compounds of Silver(I) N-(4-pyridyl) Benzamide with varying counterions, namely [Ag(NPBA)2]X, where NPBA is N-(4-pyridyl) Benzamine, X = NO3- (1), ClO4- (2), CF3SO3- (3), PF6- (4), BF4- (5), CH3PhSO3- (6), and PhSO3- (7), which form extended - stacking networks in one-dimensional (1D), 2D and 3D directions in the crystalline solid-state via the phenyl moiety, with average inter-ring distances of 3.823 Å. Interestingly, the counterions that contain …