Biomedical Commons^™

Nanopulse Generators: Their Design And Application To Cancer Therapy Studies, Daniel P. Wernig

Senior Honors Theses

Effective nanopulse generators have become critical in recent decades concerning the study of subcellular affects in response to nanosecond pulsed electric fields. It has been observed that nanosecond duration electric pulses can target intracellular organelles, ultimately leading to cell apoptosis, suggesting the possibility of a new, minimally invasive, low risk cancer therapy methodology. The standard topology for developing a medical nanopulser is the Blumlein “transmission line” approach. This approach relies on the nearly infinitesimal, yet finite amount of time required for an electromagnetic field to propagate down a short transmission line. Prior to design, requirements and constraints must be defined …

Shape/Image Registration For Medical Imaging : Novel Algorithms And Applications., Ahmed Magdy Shalaby 1982-

Electronic Theses and Dissertations

This dissertation looks at two different categories of the registration approaches: Shape registration, and Image registration. It also considers the applications of these approaches into the medical imaging field. Shape registration is an important problem in computer vision, computer graphics and medical imaging. It has been handled in different manners in many applications like shapebased segmentation, shape recognition, and tracking. Image registration is the process of overlaying two or more images of the same scene taken at different times, from different viewpoints, and/or by different sensors. Many image processing applications like remote sensing, fusion of medical images, and computer-aided surgery …

Induction Of Differentiation Of Dental Pulp-Derived Mesenchymal Stem Cells (Dpsc), Aubrey Young

UNLV Theses, Dissertations, Professional Papers, and Capstones

Mesenchymal stem cells are derived from a variety of human tissues and are being bioengineered and studied for possible uses in the advancement of medicine. Recent efforts are being focused on Dental Pulp Stem Cells (DPSC's) due to the accessibility of this tissue. Many factors influence DPSC quality and quantity, including the specific methods used to isolate, collect, concentrate, and store these isolates once they are removed. Ancillary factors, such as the choice of media, the selection of early versus late passage cells, and cryopreservation techniques may also influence the differentiation potential and proliferative capacity of DPSC isolates.

The objective …

Data Completion Methods For Improved Developmental Stage Annotation Of Drosophila Embryos In Images, Chitsanu Janyalikit

Electrical & Computer Engineering Theses & Dissertations

Drosophila melanogaster is a dominant model organism for studying the function of animal genes in initial stages of embryogenesis. Usually, images containing Drosophila gene expression patterns are captured at different developmental stages to study the interconnection of animal genes. To achieve most biologically meaningful results, gene expression images from a similar stage should be compared. Currently, biologists manually classify embryos in images into different stages, which is time intensive and infeasible for current massively produced gene expression images. Therefore, there is a need to develop an automatic system for the annotation.

Gene expression information in embryo images usually appears as …

Cited2-Mediated Mechanotransduction And Its Use For Chondroprotection, Daniel J. Leong

Dissertations, Theses, and Capstone Projects

Novel prevention and therapeutic treatment for cartilage degradation is urgently called for, as cartilage degradation is a hallmark for arthritic diseases including osteoarthritis (OA) and rheumatoid arthritis (RA), and there is a high incidence of arthritis-related disability and high medical costs. While both underuse (e.g. physically inactive lifestyle) and overuse (e.g. high impact or intense repetitive joint use as seen in certain sports) are risk factors for cartilage degradation, recent studies highlight that dynamic moderate loading is associated with reduced incidence of developing OA. Exercise is prescribed in most cases at moderate levels for both OA and RA patients, and …

Detection Of Parkinson Disease Rest Tremor, Matthew J. Johnson

McKelvey School of Engineering Graduate Student Theses & Dissertations

Parkinson Disease (PD) is a debilitating and progressive movement disorder that is estimated to affect over six million worldwide. One of the most characteristic symptoms of PD is resting tremor, which involves unintentional and rhythmic muscle oscillations of an afflicted extremity while the muscles of said extremity are relaxed. This study involved measuring the rest tremor of 10 PD subjects, 10 Essential Tremor subjects, and 10 healthy control subjects using two devices. One device was an FDA approved accelerometry system to measure human tremor known as the Tremorometer^TM and the other was a consumer three-dimensional camera known as the …

Medical Applications Of Mrc, Kyle Thackston, Henry Mei, Pedro Irazoqui

The Summer Undergraduate Research Fellowship (SURF) Symposium

Consistent powering is a limiting reagent for many medical implants and sensors. Powering in-vivo devices in animal studies requires either transcutaneous wiring (limiting mobility and increasing the chance of infection) or an implanted battery (limited lifetime and limits size of device). Wireless power transfer (WPT) would be able to overcome these challenges and permit the use of more advanced implantable devices in a research setting. Magnetic resonance coupling (MRC), an advanced form of inductive charging, allows good transfer efficiencies over significant air gaps, but works best a specific location and frequency, limiting mobility in animal studies. Using band-pass filter theory, …

Towards Highly-Integrated Stereovideoscopy For In Vivo Surgical Robots, Jay Carlson

Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research

When compared to traditional surgery, laparoscopic procedures result in better patient outcomes: shorter recovery, reduced post-operative pain, and less trauma to incisioned tissue. Unfortunately, laparoscopic procedures require specialized training for surgeons, as these minimally-invasive procedures provide an operating environment that has limited dexterity and limited vision. Advanced surgical robotics platforms can make minimally-invasive techniques safer and easier for the surgeon to complete successfully. The most common type of surgical robotics platforms -- the laparoscopic robots -- accomplish this with multi-degree-of-freedom manipulators that are capable of a diversified set of movements when compared to traditional laparoscopic instruments. Also, these laparoscopic robots …

Design, Characterization And Application Of A Multiple Input Stethoscope Apparatus, Spencer Geng Wong

Master's Theses

For this project, the design, implementation, characterization, calibration and possible applications of a multiple transducer stethoscope apparatus were investigated. The multi-transducer sensor array design consists of five standard stethoscope diaphragms mounted to a rigid frame for a-priori knowledge of their relative spatial locations in the x-y plane, with compliant z-direction positioning to ensure good contact and pressure against the subject’s skin for reliable acoustic coupling. When this apparatus is properly placed on the body, it can digitally capture the same important body sounds investigated with standard acoustic stethoscopes; especially heart sounds. Acoustic signal inputs from each diaphragm are converted to …

Cfd Simulation Of The Thermal Performance Of A Parallel Counter-Parallel Flow Heat Exchanger For The Treatment Of Hypothermia, Alex Heller

UNLV Theses, Dissertations, Professional Papers, and Capstones

Hypothermia is a life-threatening condition. Currently, active warming methods are the most effective treatment for dysthermic patients. The aim of this study is to investigate the use of computational fluid dynamics (CFD) in evaluating the thermal performance of a parallel/counter-parallel flow heat exchanger used as part of a fluid warmer to treat Hypothermia. The 3D model of the heat exchanger is divided into three regions; Infusate (fluid to be heated), Hot Water (heating fluid), and a Solid Region (wall). At the end of the heat exchanger, an elbow section is used to create the counter-parallel flow arrangement specific to this …

Improving Golf Putt Performance With Statistical Learning Of Eeg Signals, Qing Guo

Graduate Theses and Dissertations

In this thesis, a machine learning based method is proposed to predict the putt outcomes of golfers based on their electroencephalogram (EEG) data. The method can be used as a core building block of a brain-computer interface, which is designed to provide guidance to golf players based on their EEG patterns. The proposed method includes three steps. First, multi-channel 1-second EEG trials were extracted during golfers' preparation of putting. Second, different features are calculated such as correlation coefficient, power spectrum density and coherence, which are used as features for the classification algorithm. To predict golfers' performance, the support vector machine …

Preliminary Test Of A Real-Time, Interactive Silent Speech Interface Based On Electromagnetic Articulograph, Jun Wang, Ashok Samal, Jordan R. Green

School of Computing: Conference and Workshop Papers

A silent speech interface (SSI) maps articulatory movement data to speech output. Although still in experimental stages, silent speech interfaces hold significant potential for facilitating oral communication in persons after laryngectomy or with other severe voice impairments. Despite the recent efforts on silent speech recognition algorithm development using offline data analysis, online test of SSIs have rarely been conducted. In this paper, we present a preliminary, online test of a real-time, interactive SSI based on electromagnetic motion tracking. The SSI played back synthesized speech sounds in response to the user’s tongue and lip movements. Three English talkers participated in this …

St. Jude Medical - R&D Robot For Sensor Testing, Andrew Chanul Kim, Stanley Logan Laszczyk, Edgar Nava, Nathan Gall

Electrical Engineering

No abstract provided.

Alternate Computer Input Device For Individuals With Quadriplegia, Tobais Elder, Michelle Martinez, David Sylvester

Biomedical Engineering

This project details the design development of an alternative computer input system that allows a person with quadriplegia to move a computer's cursor and activate left and right click button inputs. After researching and analyzing possible solutions, an end design was chosen that most appropriately satisfied all user requirements and engineering specifications. This final design employs a head mounted Inertial Measurement Unit (IMU) with 9 DoF (Degrees of Freedom) to track head movements and correlate these motions to computer cursor movements. A Sip-Puff Transducer monitors and interprets a user's application of negative and positive air pressure differentials to a vinyl …

Sonar For The Visually Impaired, Anastasia Newark, Edwin Ng, Scott Terhorst

Biomedical Engineering

There are currently no inexpensive assistive devices that allow the visually impaired detect low hanging objects that are above waist height and may cause injury, such as a rope or tree branch. The challenge presented to the team by the Quality of Life Plus Lab (QL+) was to develop a Sonar Cane device that attaches to a traditional white cane to address this problem. This device must be lightweight, inexpensive to manufacture, have a long battery life, and accurately detect obstacles to prevent user injury.

Reading And Wirelessly Sending Eeg Signals Using Arduinos And Xbee Radios To Control A Robot, Andrew Paul Simms

Electrical Engineering Undergraduate Honors Theses

The objective of this project is to develop an EEG device that can read brainwaves from an individual, analyze the data, and use the result to send a wireless signal using Arduinos and XBee Radios to a Boe-bot to perform an action. One of the goals of this project is to read EEG data with a higher sampling frequency than a previously manufactured EEG device. The second part of the project used the device developed to differentiate an individual’s thinking between right and left and then send a simple signal to a robot using an XBee radio to perform an …

Generation Of Bipolar Nanosecond Pulses, Sambasiva Rao Rajulapati

Electrical & Computer Engineering Theses & Dissertations

Bipolar pulse generation has been studied using transmission line technology, which was mainly used for monopolar pulse generation. In this thesis, low impedance, high current, nanosecond pulse generators were designed, constructed and tested. Five transmission lines were connected in parallel, so the impedance was 10 n. The transmission lines were switched by a spark gap switch operated in air. The closing of the switch creates a matched condition on one side of the transmission lines and a short circuit on the other side of the transmission lines. When the transmission lines are precharged, one of the two waves in opposite …

Its All In The Mind: Mind Controlled Prosthetics, Tziril Joselit

The Science Journal of the Lander College of Arts and Sciences

The problem with prosthetics is a longstanding problem that researchers have been working on for many years. They are attempting to create a prosthetic that acts as if it is the original limb or body part. In recent years they have discovered a technology that has assisted many of those who are greatly in need of a prosthetic, such as an amputee or someone who is “locked in”. “Locked in” refers to a person who is technically confined in his own body and has no methods of communication with the world. Brain-computer interface (BCI) has opened up a whole new …

Measuring Pain Withdrawal Threshold Using A Novel Device In "Pseudo-Continuous" Mode, A. H. Walker, S. Thurman, N. Martinez, S. Burns, M. Dobretsov

Journal of the Arkansas Academy of Science

The study of pain and analgesia is an important area of biomedical research that has led to a significant number of advances in the treatment of acute and chronic pain. This study introduces a novel approach to mechanical testing of pain withdrawal of a rat hind paw to a stimulus. This systematic method involves a modified electronic esthesiometer controlled by an IDEA drive that allows for consistency in experiments. The device gives the experimenter computer control of the step size and velocity of approach of the probe stimulus. We discuss here some of the limitations in the current techniques used …

Inkjet Bioprinting Of Solid Peroxides For Constructing Oxygen Generating Scaffolds To Improve Cells Viability And Growth Under Hypoxic Environment, Daniel Reyna Soriano

Open Access Theses & Dissertations

Tissue engineering has emerged as an interdisciplinary field to overcome current challenges for tissue repair or replacement in the human body. In essence, it proposes new medical therapies customized to match the biology of specific patients. The potential benefits offered by tissue engineering have driven scientific inquiry to make it a clinical reality.

One of the current challenges in tissue engineering is to provide oxygen supply to thick tissues as oxygen diffusion is limited to 100 - 200 µm layer of viable tissue. Engineered vascular conduits are applied in vitro, providing pre-vascularization to tissues; however, host anastomosis is still a …

Calcium-Mediated Pore Expansion And Cell Death Following Nanoelectroporation, Olga N. Pakhomova, Betsy Gregory, Iurii Semenov, Andrei G. Pakhomov

Bioelectrics Publications

Opening of long-lived pores in the cell membrane is the principal primary effect of intense, nanosecond pulsed electric field (nsPEF). Here we demonstrate that the evolution of pores, cell survival, the time and the mode of cell death (necrotic or apoptotic) are determined by the level of external Ca²⁺ after nsPEF. We also introduce a novel, minimally disruptive technique for nsEP exposure of adherent cells on indium tin oxide (ITO)-coated glass coverslips, which does not require cell detachment and enables fast exchanges of bath media. Increasing the Ca²⁺ level from the nominal 2–5 μM to 2 mM for …

Introduction To Fourth Special Issue On Electroporation-Based Technologies And Treatments, Damijan Miklavčič, Lluis M. Mir, P. Thomas Vernier

Bioelectrics Publications

This fourth special electroporation-based technologies and treatments issue of the Journal of Membrane Biology contains reports on recent developments in the field of electroporation by participants in the 7th International Workshop and Postgraduate Course on electroporation based technologies and treatments (EBTT 2013) held in Ljubljana, November 17–23, 2013. The 65 participants included faculty members, invited lecturers, special guests, and young scientists, and students from 16 countries. In addition to lectures on the fundamentals, this year’s sessions included talks on microbial inactivation by pulsed electric fields, modeling of intracellular electroporation, electroporation in food processing, and electrotransfer-facilitated DNA vaccination.

Nuclei/Cell Detection In Microscopic Skeletal Muscle Fiber Images And Histopathological Brain Tumor Images Using Sparse Optimizations, Hai Su

Theses and Dissertations--Computer Science

Nuclei/Cell detection is usually a prerequisite procedure in many computer-aided biomedical image analysis tasks. In this thesis we propose two automatic nuclei/cell detection frameworks. One is for nuclei detection in skeletal muscle fiber images and the other is for brain tumor histopathological images.

For skeletal muscle fiber images, the major challenges include: i) shape and size variations of the nuclei, ii) overlapping nuclear clumps, and iii) a series of z-stack images with out-of-focus regions. We propose a novel automatic detection algorithm consisting of the following components: 1) The original z-stack images are first converted into one all-in-focus image. 2) A …

Concept Design Of A Labview-Mysql Data Warehouse For Digital Recording Of Ecg-Emg, Kristine R. Suratos, Rosula Sj Reyes

Electronics, Computer, and Communications Engineering Faculty Publications

To align with the recommendations set forth by the World Health Organization regarding eHealth, this study aims to develop a concept design of a suitable data warehouse for healthcare using electronic medical recording of ECG-EMG. This concept design once implemented aims to assist health care providers in improving patient’s healthcare data management. The system consists of three main layer: data source layer; software application layer; and data service database layer. The data source layer consists of patient profile, patient consultation, and patient test. Patient test was conducted using Olimex ECG-EMG shield and NI myDAQ module. The software application layer, developed …

Temporal Modulation Of Plasma Species In Atmospheric Dielectric Barrier Discharges, Aijun Yang, Xiaohua Wang, Dingxin Liu, Mingzhe Rong, Michael G. Kong

Bioelectrics Publications

The atmospheric pressure dielectric barrier discharge in helium is a pulsed discharge in nature and the moment of maximum species densities is almost consistent with peak discharge current density. In this paper, a one-dimensional fluid model is used to investigate the temporal structure of plasma species in an atmospheric He-N₂ dielectric barrier discharge (DBD). It is demonstrated that there exist microsecond delays of the moments of the maximum electron and ion densities from the peak of discharge current density. These time delays are caused by a competition between the electron impact and Penning ionizations, modulated by the N_{2 …}

Examining The Role Of Attention In Steering Using A Dual Task Paradigm, Venkata Naga Pradeep Ambati

Open Access Theses & Dissertations

Changing the direction of locomotion, often referred to as "steering", is an integral component of human locomotion. Steering requires maintaining dynamic balance while translating and rotating the body in the new travel direction. Given the level of sensation thought to be involved in sub serving goal directed modifications in steering, it is highly likely that steering control may require attention. Since attention resources decline with increasing age, we may see the influence of age on steering performance when attention resources are limited. Therefore, this study sought to investigate the role of attention in steering using a dual task paradigm in …

Cell Releasing System Using Light Responsive Photovoltaic Devices, Mohammod Khairul Kabir Bhuyan

Open Access Theses & Dissertations

Tissue and organ regeneration via transplantation of cell bodies in-situ has become an emerging strategy in regenerative medicine. Injected or implanted cells must be available in large enough numbers and suitable delivery methods must be found especially when implantation into the affected tissue is not possible. In addition, better understanding of how to mitigate the inflammatory reactions and how to degrade the cell carriers are needed. Current cell-based therapies are limited to injection or implantation of cell suspensions or implantation of cell sheets. Development of alternative systems to obtain cell sheets or other functional cellular structures are required. We are …

Optimized Integrated Software Package For Classification Of Small Airway Dysfunction Using Genetic Algorithms, Elias Estrada

Open Access Theses & Dissertations

Due to the similarity in the symptoms of respiratory disorders, an accurate diagnosis could be a difficult task. Regarding asthma as the main focus of this research, early detection of small airway impairment could represent a better opportunity for timely diagnosis. An integrated software package (ISP) based on the impulse oscillometry technique, has been developed to serve as an important tool for clinicians during classification of asthma severities on children [1]. The current version of the ISP was tested with data obtained during several years by Dr. Homer Nazeran and Dr. Michael Goldman from 110 Hispanic children [2], providing an …

Design And Development Of Implantable And Remote Controlled Distraction Osteogenesis Device For Limb Lengthening Practice In Children, Mario Eduardo Rodriguez De La O

Open Access Theses & Dissertations

Recent technology improvements have been positively affecting medical industry in recent years since procedures and equipment are more precise and reliable. Nevertheless, there are still surgical procedures and medical devices that not fully address specific needs according to medical experts. One of those necessities is focused on options available for children patients when subjected to a surgical procedure named distraction osteogenesis. In general, distraction osteogenesis is performed when skeletal deformities need to be corrected or when bone lengthening is required, typically a mechanism is utilized to elongate bone at a controlled rate. The existing osteodistraction methods for limb lengthening and …

Use Of Cosine Modulated Filter Bank To Quantify Human Muscle Fatigue Using Electromyography Signals Obtained During Isometric Voluntary Contractions, Liza Stephanie Rodriguez

Open Access Theses & Dissertations

As technology continues to advance, there continues to be an interest in applying engineering theories to solve medical problems. Technology has allowed researchers to investigate the properties and characteristics of the human body in hopes of emulating their complexities. The rising interest in the study of biopotentials for use in prosthetic limbs, exoskeletons and machine interfacing has led to a necessity to validate that the understanding of these signals is accurate. The study of biopotentials generated by muscles via a method known as electromyography (EMG) has improved the understanding of effects of rehabilitative treatments, assisted in diagnosing diseases, control of …