Biomedical Commons^™

Small-Separation Speckle Contrast Optical Spectroscopy For Intraoperative Assessment Of Parathyroid Glands Viability During Thyroid Surgery, Connor Berger

Symposium of Student Scholars

The parathyroid glands (PTGs) are often damaged during thyroid surgeries due to a lack of methods identifying PTGs and assessing their viability. Damage to PTGs can cause hypocalcemia, a deficiency of calcium in the body. This complication can lead to detrimental consequences with economic burden. The surgeon’s current method of viability assessment is qualitative and subjective. Our technical solution is to employ an optical technique called speckle contrast optical spectroscopy (SCOS) that noninvasively quantifies the blood flow index (Db) of biological tissues at deep tissue levels (>1cm). The goal of this project is to verify SCOS at small source-detector-separation …

Fiber-Based Time-Correlated Single Photon Counting As A Method For Investigating Metabolism During Tetanic Contraction Of Skeletal Muscle, Kathryn Miller Priest

Graduate Theses and Dissertations

Nicotinamide adenine dinucleotide (NAD) is a cofactor that serves to shuttle electrons during metabolic processes such as glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation (OXPHOS) [1-5]. The reduced form of NAD, NADH, is autofluorescent, making it a measurable characteristic in the investigation of metabolic changes. The fluorescence lifetime of NADH is dependent on the state of protein binding, specifically free or bound NADH, allowing for detection of metabolic shifts between glycolysis (free NADH) and OXPHOS (bound NADH) [6-16]. Fiber-coupled time-correlated single photon counting (TCSPC) equipped with an implantable needle probe can be used to measure NADH lifetime in vivo …

An Integrated Electronic-Skin Patch For Real-Time And Continuous Monitoring Of A Panel Of Biomarkers Combined With Drug Delivery, Tanzila Noushin

Electrical Engineering Theses

Inflammatory biomarkers present in the human body play a vital role in medical field by guiding the clinician in decision-making for many diseases. The levels of these inflammatory biomarkers are associated with the severity and progress of several diseases. Researchers have found that increasing severity of many diseases such as cardiovascular disease, after surgery infection, and adverse clinical outcomes due to infectious diseases, results in the elevation of the level of inflammatory biomarkers in human sweat. Furthermore, the inflammatory cytokines indicate the pathophysiology and prognosis of critically ill SARS‑CoV‑2 patients. In this thesis work, different sensors have been developed for …

Engineering 3d Bioprinted Cardiac Spheroidal Droplets With Cardiomyocytes And Cardiac Fibroblasts For Tissue Engineering And Drug Cytotoxicity Studies, Raven El Khoury

Open Access Theses & Dissertations

Engineering is the supreme human endeavor that involves harnessing the scientific understanding of the natural world to design and invent objects to improve the society around us. Biomedical engineering is the implementation of concepts acquired from engineering in biology and medicine that aims to improve human health through the integration of engineering with biomedical sciences. The mission of a biomedical engineer is to develop technologies that help advance the quality of peopleâ??s health using various tools and materials with one passion and goal: making the patient's life longer and easier. Tissue engineering is developed from the field of biomaterials and …

Identifying And Minimizing Underspecification In Breast Cancer Subtyping, Jonathan Cheuk-Kiu Tang

Master's Theses

In the realm of biomedical technology, both accuracy and consistency are crucial to the development and deployment of these tools. While accuracy is easy to measure, consistency metrics are not so simple to measure, especially in the scope of biomedicine where prediction consistency can be difficult to achieve. Typically, biomedical datasets contain a significantly larger amount of features compared to the amount of samples, which goes against ordinary data mining practices. As a result, predictive models may fail to find valid pathways for prediction during training on such datasets. This concept is known as underspecification.

Underspecification has been more accepted …

Diy Cell Incubator, Hayden James Jeanor

Electrical Engineering

The purpose of creating a cell Incubator is for the development of cell and tissue production in laboratory settings. Large scale research projects and the medical community grow cells for various reasons, including experiments and creating tissue for patients. However, they cannot simply depend on growing cells in a petri dish that sit on a rack at room temperature. To grow heathy cells in the fastest way possible, they use cell incubators. Cell incubators create an atmosphere within the incubation bay that is designed to promote cell growth. The three main components that need to be constantly regulated, using a …

Digitally Predicting Protein Localization And Manipulating Protein Activity In Fluorescence Images Using 4d Reslicing Gan, Yang Jiao, Lingkun Gu, Yingtao Jiang, Mo Weng, Mei Yang

Electrical & Computer Engineering Faculty Research

While multi-channel fluorescence microscopy is a vital imaging method in biological studies, the number of channels that can be imaged simultaneously is limited by technical and hardware limitations such as emission spectra cross-talk. One solution is using deep neural networks to model the localization relationship between two proteins so that the localization of one protein can be digitally predicted. Furthermore, the input and predicted localization implicitly reflect the modeled relationship. Accordingly, observing the response of the prediction via manipulating input localization could provide an informative way to analyze the modeled relationships between the input and the predicted proteins.

Material Characterization And Comparison Of Sol-Gel Deposited And Rf Magnetron Deposited Lead Zirconate Titanate Thin Films, Katherine Lynne Miles

Mechanical Engineering ETDs

Lead zirconate titanate (PZT) has been a material of interest for sensor, actuator, and transducer applications in microelectromechanical systems (MEMS). This is due to their favorable piezoelectric, pyroelectric and ferroelectric properties. While various methods are available to deposit PZT thin films, radio frequency (RF) magnetron sputtering was selected to provide high quality PZT films with the added capability of batch processing. These sputter deposited PZT films were characterized to determine their internal film stress, Young’s modulus, composition, and structure. After characterization, the sputtered PZT samples were poled using corona poling and direct poling methods. As a means of comparison, commercially …

Decellularization Strategies Of Naturally Derived Biomaterials For Tissue Engineering Applications, Julia Elizabeth Hohn

Theses and Dissertations

In 2017, over 3.5 million peripheral vascular surgeries were performed worldwide with over 400,000 vascular repair or replacement surgeries being performed in the United States each year alone. As the number of vascular repair surgeries, including both coronary and peripheral bypass grafting procedures, continues to increase each year, these statistics indicate an urgent need for more effective and readily available replacement materials. Regenerative medicine and tissue engineering (TE) approaches, including the design, fabrication, and validation of suitable biomaterials in vitro that direct the repair and regeneration of damaged tissues, have been proposed to alleviate this problem. While advanced biomaterials have …

Embedding A Grid Of Load Cells Into A Dining Table For Automatic Monitoring And Detection Of Eating Events, Mohammad Mayyan

All Dissertations

This dissertation describes a “smart dining table” that can detect and measure consumption events. This work is motivated by the growing problem of obesity, which is a global problem and an epidemic in the United States and Europe. Chapter 1 gives a background on the economic burden of obesity and its comorbidities. For the assessment of obesity, we briefly describe the classic dietary assessment tools and discuss their drawback and the necessity of using more objective, accurate, low-cost, and in-situ automatic dietary assessment tools. We explain in short various technologies used for automatic dietary assessment such as acoustic-, motion-, or …

Design Of A Hemi-Anechoic Chamber For Acoustic Testing Of Hearing Devices And Development Of Custom-Designed 3d Printed Pinnae For Accurate Representation Of The Anatomical Frequency Response, Hector Hugo Estrada Medinilla

Open Access Theses & Dissertations

Sound localization is the capacity to identify the general location from which a sound is originating. The primary anatomical feature responsible for the ability to localize sound is the external portion of the ear, also known as the auricle or pinna. This faculty is significantly hindered when the ear is obstructed, as is the case with hearing protection devices (HPD), which act as a barrier between sound waves and the ear canal. In the case of electronic HPDs, certain frequencies are entirely filtered out by a digital sound processor (DSP) while the remaining frequencies are delivered directly into the ear …

Fetal Electrocardiogram Detection And Analysis From Maternal Abdominal Ecg For Diagnosis Of Fetal Heart Rhythm Abnormalities, Claudia L. Angel

Open Access Theses & Dissertations

Fetal cardiac arrhythmias are present in about 1–3% of pregnancies and account for about 10–20% of the referrals to fetal cardiology around the world. Most fetal arrhythmias are benign; however, some can cause fetal hydrops, preterm delivery, and higher perinatal morbidity and mortality. The evolution and treatment of fetal arrhythmia depend on a timely and complete diagnosis. However, conventional methods used in clinical practice for fetal arrhythmia diagnosis are limited since they do not reflect the primary electrophysiological conduction processes in the myocardium. Fetal electrocardiography (fECG) has the potential to better support fetal arrhythmia diagnosis through the continuous analysis of …

Development Of A Custom, 3d-Printed, Multi-Microphone, Noise-Cancelling, Hearing Protection Device With A Magnetically Attached Printed Ear Canal For Sound Localization Preservation, Brenda Leticia Valadez Mesta

Open Access Theses & Dissertations

Hearing loss is a prominent health issue that affects a significant fraction of the global population. One of the main causes behind hearing loss is exposure to harmful noise levels in the workplace. There have been multiple efforts to prevent hearing loss from excessive noise in occupational settings, such as hearing conservation programs and use of hearing protection devices (HPDs), however, these are often ineffective. Although HPDs are frequently available in the workspace, they are not used as intended since they usually generate discomfort and hindered sound localization. The loss of sound localization can be hazardous in occupational settings, since …

Computer Aided Diagnosis System For Breast Cancer Using Deep Learning., Asma Baccouche

Electronic Theses and Dissertations

The recent rise of big data technology surrounding the electronic systems and developed toolkits gave birth to new promises for Artificial Intelligence (AI). With the continuous use of data-centric systems and machines in our lives, such as social media, surveys, emails, reports, etc., there is no doubt that data has gained the center of attention by scientists and motivated them to provide more decision-making and operational support systems across multiple domains. With the recent breakthroughs in artificial intelligence, the use of machine learning and deep learning models have achieved remarkable advances in computer vision, ecommerce, cybersecurity, and healthcare. Particularly, numerous …

Miniaturized Battery Powered Air Quality Monitoring System, Bryan Chacon

All Theses

In this work, an air quality monitoring system was developed using various sensors that measure specific air quality parameters, including Volatile Organic Compounds, Carbon Dioxide, particulate matter of varying sizes, ambient pressure, humidity, and temperature. This system is based off a Particle micro-controller, Boron LTE CAT-M1 which allows for cellular connectivity for real-time data transmission. It is powered by a 3.7 Volt Li-Po battery and has a miniaturized design which allows for portability. This data is processed through an Internet of Things software provider that allows for the device to be connected and accessed to and from anywhere in the …

Design And Operation Of A Microwave Flow Cytometer For Single Cell Detection And Identification, Jeffrey A. Osterberg

All Dissertations

Microwave dielectric sensing has become a popular technique in biological cell sensing for its potential in online, label-free, and real-time sensing. At microwave frequencies probing signals are sensitive to intracellular properties since they are able to penetrate cell membranes, making microwave flow cytometry a promising technology for label-free biosensing. In this dissertation a microwave flow cytometer is designed and used to measure single biological cells and micro particles. A radio frequency (RF)/microwave interferometer serves as the measurement system for its high sensitivity and tunability and we show that a two-stage interferometer can achieve up to 20 times higher sensitivity than …

Credit Card Fraud Detection Using Machine Learning Techniques, Nermin Samy Elhusseny, Shimaa Mohamed Ouf, Amira M. Idrees Ami

Future Computing and Informatics Journal

This is a systematic literature review to reflect the previous studies that dealt with credit card fraud detection and highlight the different machine learning techniques to deal with this problem. Credit cards are now widely utilized daily. The globe has just begun to shift toward financial inclusion, with marginalized people being introduced to the financial sector. As a result of the high volume of e-commerce, there has been a significant increase in credit card fraud. One of the most important parts of today's banking sector is fraud detection. Fraud is one of the most serious concerns in terms of monetary …

Sex Differences And Potential Non-Invasive Treatments For Calcific Aortic Valve Disease, Henry Pascal Helms

Theses and Dissertations

Calcific Aortic Valve Disease (CAVD) is a progressive heart disease that ranges from aortic valve sclerosis to aortic valve stenosis. It is characterized by intense calcification and compromised valve function. CAVD affects 25% of people older than 65 and 50% of people older than 85. These rates are expected to increase in the United States due to higher levels of obesity and diabetes, as well as an aging population. CAVD is the leading cause of valve replacement surgery. Annual healthcare costs for these valve replacements are currently estimated to be approximately 2 billion dollars. There are currently no medications approved …

Smart Bottle Ble Integration, Joshua M. Rizzolo

Electrical Engineering

In 1975, four percent of children aged five to nineteen were categorized as overweight or obese. As of 2016, this figure climbed above 18 percent [1]. Researchers at California Polytechnic State University San Luis Obispo (Cal Poly) want to investigate the effect of overeating in early childhood on later childhood obesity. This research requires collecting feeding pattern data on infants, which proves challenging. Parents cannot be relied on to regularly collect clean data due to factors including work schedule, multitasking, and general exhaustion. Thus, we have developed a tool to automatically collect data on feeding frequency and duration, as well …

Bicep Muscle Rep Counter With Semg, Matthew Max Garcia

Electrical Engineering

The Bicep Muscle Rep Counter with SEMG is a device that was made to develop three repetition thresholds or difficulty levels from a fully-flexed bicep brachii muscle and signal to the user when consequent bicep muscle contractions pass said thresholds or levels. This device can be used when performing a bicep-focused movement or when generally flexing the bicep muscle. Essentially, this device serves to make sure each repetition of a muscle contraction passes a percentage value calculated from the electrical activity outputted from a max contraction.

The device is not designed for and does not produce data for muscle growth, …

Neural Network Based Diagnosis Of Breast Cancer Using The Breakhis Dataset, Ross E. Dalke

Master's Theses

Breast cancer is the most common type of cancer in the world, and it is the second deadliest cancer for females. In the fight against breast cancer, early detection plays a large role in saving people’s lives. In this work, an image classifier is designed to diagnose breast tumors as benign or malignant. The classifier is designed with a neural network and trained on the BreakHis dataset. After creating the initial design, a variety of methods are used to try to improve the performance of the classifier. These methods include preprocessing, increasing the number of training epochs, changing network architecture, …

Improving The Performance And Evaluation Of Computer-Assisted Semen Analysis, Ji-Won Choi

Dissertations

Semen analysis is performed routinely in fertility clinics to analyze the quality of semen and sperm cells of male patients. The analysis is typically performed by trained technicians or by Computer-Assisted Semen Analysis (CASA) systems. Manual semen analysis performed by technicians is subjective, time-consuming, and laborious, and yet most fertility clinics perform semen analysis in this manner. CASA systems, which are designed to perform the same tasks automatically, have a considerable market share, yet many studies still express concerns about their accuracy and consistency. In this dissertation, the focus is on detection, tracking, and classification of sperm cells in semen …

Assessing Structural And Functional Brain Alterations And Work-Related Fatigue In Non-Hyposmic And Hyposmic Covid-19 Survivors, Rakibul Hafiz

Dissertations

In the year 2019, life began to change at the advent of a global pandemic caused by the novel coronavirus. Mask mandates and mass vaccinations have mitigated the effects significantly, yet cases keep rising with new variants, especially, in densely populated countries, like India. Recent neuroimaging evidence shows the virus can attack the central nervous system (CNS). However, exactly which brain regions undergo structural and functional changes remain largely unknown. Many patients experience 'loss of/reduced sense of smell' (i.e., hyposmic) and an alarming number of survivors develop persistent symptoms ('long-COVID') for several months after initial infection. Fatigue is the most …

Closed-Loop Brain-Computer Interfaces For Memory Restoration Using Deep Brain Stimulation, David Xiaoliang Wang

Electrical Engineering Theses and Dissertations

The past two decades have witnessed the rapid growth of therapeutic brain-computer interfaces (BCI) targeting a diversity of brain dysfunctions. Among many neurosurgical procedures, deep brain stimulation (DBS) with neuromodulation technique has emerged as a fruitful treatment for neurodegenerative disorders such as epilepsy, Parkinson's disease, post-traumatic amnesia, and Alzheimer's disease, as well as neuropsychiatric disorders such as depression, obsessive-compulsive disorder, and schizophrenia. In parallel to the open-loop neuromodulation strategies for neuromotor disorders, recent investigations have demonstrated the superior performance of closed-loop neuromodulation systems for memory-relevant disorders due to the more sophisticated underlying brain circuitry during cognitive processes. Our efforts are …

Predicting The Progression Of Diabetes Mellitus Using Dynamic Plantar Pressure Parameters, Mathew Sunil Varre

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: Diabetic peripheral neuropathy is one of the common complications of type-2 diabetes mellitus (DM). Changes in the intrinsic plantar tissue coupled with repetitive mechanical loads and loss of sensation may lead to foot related complications (skin break down, ulcerations, and amputations) in persons with neuropathy if left untreated. The purpose of this dissertation was to stratify individuals with pre-diabetes, diabetes with and without neuropathy using dynamic plantar pressure parameters during walking, using machine learning algorithms.Methods: Plantar pressure data was collected from one hundred participants during walking with pressure measuring insoles fixed between the feet and thin socks. Simultaneously high-definition …

Insole Fall Prevention Device, Nick M. Hughes, Andrew M. Slaboda

Biomedical Engineering

Falls among the aging population occur every single day, with 1 in every 5 resulting in some injury and 300,000 hospitalized every year with a hip fracture [1]. The most popular and effective way to mitigate these falls is through physical therapist intervention. However, with the increased popularity in telerehab, many patients at risk for falls cannot accurately convey their gait tendencies to their physical therapists from the comfort of their home or while not in direct contact with the PT. A device like an insole, implanted with force sensors, which measures different parts of a patient’s foot, could convey …

The Mobility And Cognitive Mechanisms Involved In Altering Gait Speed In Children With Autism Spectrum Disorder, Alyssa Olivas

Open Access Theses & Dissertations

Autism is a socio-behavioral disorder, and diagnoses are conducted through behavioral screening instruments. Recent research suggests motor deficits may be a core symptom of the disorder, as children with autism present with deficits in motor development, locomotor skills, and postural instability. In addition, children with autism often have affected, executive function, attention, and perception cognitive domains. Different gait speeds have been used to examine gait adaptations in other clinical populations. Additionally, individuals with decreased cognitive abilities have demonstrated difficulties in modulating their walking speed. There is limited research on mechanisms children with autism use to alter their gait speed, or …

Radiomic Features To Predict Overall Survival Time For Patients With Glioblastoma Brain Tumors Based On Machine Learning And Deep Learning Methods, Lina Chato

UNLV Theses, Dissertations, Professional Papers, and Capstones

Machine Learning (ML) methods including Deep Learning (DL) Methods have been employed in the medical field to improve diagnosis process and patient’s prognosis outcomes. Glioblastoma multiforme is an extremely aggressive Glioma brain tumor that has a poor survival rate. Understanding the behavior of the Glioblastoma brain tumor is still uncertain and some factors are still unrecognized. In fact, the tumor behavior is important to decide a proper treatment plan and to improve a patient’s health. The aim of this dissertation is to develop a Computer-Aided-Diagnosis system (CADiag) based on ML/DL methods to automatically estimate the Overall Survival Time (OST) for …

Bms 202: Human Anatomy And Physiology Laboratory Oer Curation, Chealsye Bowley

Curated OER Collections

This OER curation is an annotated bibliography of prospective OER for the GVSU course BMS 202: Human Anatomy and Physiology Laboratory, assembled by request from the instructor.

Novel Platforms For Large-Scale Adherent Culture Of Mammalian Cells, Ashkan Yekrangsafakar

LSU Doctoral Dissertations

With recent advances in biotechnology, there is a strong and urgent need for robust platforms to culture mammalian cells on a large scale to produce biopharmaceuticals. To this end, various bioreactors have been developed over the past decades, but their capacity and efficiency are often limited by insufficient mass transfer rate and excessive shear stress. In this work, multiple novel bioreactors for the large-scale adherent culture of anchorage-dependent cells were developed.

Hollow MicroCarriers (HMC) was developed as an alternative solution for the microcarrier-based culture system in a stirred-tank bioreactor. In the conventional microcarrier technique, cells are exposed to the harmful …