Life Sciences Commons^™

Metabolic Regulation Of Cardiac Fibroblast Phenotype And Function., Collin Kleis Wells

Electronic Theses and Dissertations

Cardiac fibroblasts are central effectors of cardiac repair after myocardial infarction (MI). In response to signaling cues, they differentiate to a range of phenotypes with robust capacities to synthesize and secrete extracellular matrix (ECM) and signaling molecules. Although activated fibroblast phenotypes are associated with pronounced changes in metabolism, it remains unclear how the metabolic network upholds the effector functions of fibroblasts in the post-infarcted heart. Here, we identified that critical enzymes in the phosphoenolpyruvate (PEP) cycle, i.e. pyruvate kinase muscle-2 (PKM2) and phosphoenolpyruvate carboxykinase-2 (PCK2), are elevated in the heart after MI and examined their role in regulating post-MI remodeling …

Development Of A Machine Learning Framework For An Irrigation Decision Support System, Eric Wilkening, Derek M. Heeren, Yeyin Shi, Laila A. Puntel, Guillermo R. Balboa, Abia Katimbo, Kuan Zhang, Precious Nneka Amori, Bruno Lena

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Currently used irrigation scheduling techniques often require significant human intervention and are time consuming, particularly for variable rate irrigation. This research aimed to develop a conceptual framework that incorporates disparate data sources leveraging both mechanistic and machine learning (ML) approaches. The overarching objective is to utilize the growing availability of data and technology to manage irrigation more precisely, which will minimize negative impacts of irrigation on our water resources. An initial irrigation machine learning model was proposed and tested in this study as a key component of an edge-cloud computing and federated learning decision-making framework. The specific objectives were (1) …

Intra-Stand Movement Of Balsam Woolly Adelgid (Adelges Piceae) In The Intermountain West, Michael S. Wayman

All Graduate Theses and Dissertations, Fall 2023 to Present

Balsam woolly adelgid (BWA, Adelges piceae (Ratz.)) is an invasive insect of true firs (Abies spp.). BWA has spread from initial introductions on the west coast of North America in the 1920s through the Intermountain West and has recently invaded Utah. White fir (A. concolor) and subalpine fir (A. lasiocarpa) are the two main species of concern in this region; however, subalpine fir has been found to be more susceptible to attack and subsequent damage. While long-range dispersal mechanisms and patterns are of interest for spread across regions and for predicting new infestations, very little …

The Social Dimensions Of Aquaculture: Food Sovereignty And Indigenous Rights In Ketchikan, Alaska, Morgan J. Davis

Capstone Collection

This research project asks how the growing commercial aquaculture industry in Ketchikan, Alaska, functions to alleviate or exacerbate local food insecurity; reinforce or undermine food sovereignty among Indigenous communities, and enhance or restrict community participation in the control of marine resources. Global initiatives have responded to food insecurity through agricultural inputs and technology to create higher yields; however, conventional agricultural production has contributed to and is increasingly vulnerable to climate change. To address higher demands for food, government agencies and development experts have looked towards Blue Growth and aquaculture as a solution for food insecurity and environmental concerns. While aquaculture …

Owl Integrations Soil Monitor, Derek Chau, Armaan Kahlon

Electrical Engineering

This paper examines the design process and results concerning the OWL Integrations soil monitor. Disaster relief relies heavily on digital communications, especially in environments where conventional methods (e.g. Wi-Fi, cellular, fiber optics) are unusable. To help emergency services assess disaster-stricken areas, a soil monitoring system was developed using LilyGo T-Beams (ESP32), programmed with specialized firmware known as the ClusterDuck Protocol to collect data pertaining to moisture and temperature. The project is sponsored by OWL Integrations, with help from the Cal Poly Electrical Engineering (EE) Department. Results show positive prospects, with the product able to make 4 km transmissions, operate for …

Evaluating The Effectiveness Of Individual Cleaning Steps Of A Cip Protocol In Membrane Biofilm Removal Under Dynamic Conditions, Diwaker Singh, Sanjeev Anand

Dairy Science and Food Science Faculty Publications: 2022 to Present

This study evaluated the effectiveness of individual clean-in-place (CIP) steps in removing biofilms from reverse osmosis (RO) membranes under dynamic flow conditions using the Centers for Disease Control (CDC) biofilm reactor. Biofilms were developed in the laboratory under continuous flow, using mixed-species bacterial isolates obtained from 10-month-old RO membrane biofilms from a commercial facility. Individual CIP chemicals, representative of those used in commercial protocols, were tested against 24 h-old biofilms. Additionally, a complete six-step sequential CIP process was conducted under dynamic conditions, consisting of treatments with alkali, surfactant, acid, enzyme, a secondary surfactant, and sanitizer. All experiments were performed in …

Comparative Impacts Of Irradiation On Antioxidant Compounds Of Scalded And Non-Scalded Skin Of Granny Smith Apples, Angela Banda

Food Science (MS) Theses

Post-harvest Superficial scald affects the quality of Granny Smith Apples. Oxidative stress develops superficial scald with antioxidant systems mitigating the extent of this damage. This study evaluated the effects of gamma, X-ray, and electron beam irradiation on antioxidants – ascorbic acid, total phenolics, α-tocopherol, carotenoids, chlorophyll, and campesterol – in scalded and non-scalded apple peel tissue. Apples were treated with 0.2-0.77 kGy and stored at 0-1 °C. Irradiation at the applied doses increased scald incidence, suggesting that disruption of antioxidant systems promoted reactive oxygen species accumulation and membrane damage. While certain antioxidants appeared to be upregulated as a stress response, …

Formosan Subterranean Termites Coptotermes Formosanus Shiraki (Blattodea: Heterotermitidae): An Underreported Threat To Commercial Shrimp Boats In The Midsouth, Usa, J. S. Portugal Iii

Midsouth Entomologist

No abstract provided.

Ground Truthing Of Inaturalist Submissions Confirms The Presence Of Asian Needle Ants, Brachyponera Chinensis (Emery) (Hymenoptera: Formicidae), In Louisiana, A New State Record, Aaron R. Ashbrook, Victoria Bayless, Stephen Baca, Gerardo Guevara-Milla, Chris E. Carlton

Midsouth Entomologist

Based on submissions to iNaturalist from citizen scientists, the presence of the Asian needle ant (Brachyponera chinensis), was confirmed for Louisiana in East Baton Rouge Parish. Use of citizen scientist platforms for sources of invertebrate taxa distributions is a powerful tool but requires caution because the records are typically not validated by physical voucher specimens that have been deposited in publicly accessible museums, and identification of many species is not possible by images alone. To demonstrate the usefulness of citizen scientist programs like iNaturalist, possible collection sites for Asian needle ant specimens were located. Specimens were collected, identified, and vouchers …

How Do Acoustic Indices Serve As Proxy Of Temperate Forests And In Relation To Presence Of Bobcat (Lynx Rufus) In Central Indiana Habitats, Faith Mcphee, Kristen Bellisario Dr.

Discovery Undergraduate Interdisciplinary Research Internship

Central Indiana is largely dominated by agriculture which has led to numerous historical extirpations of mammals. The bobcat (Lynx rufus) is slowly re-establishing across northern Indiana after near extirpation in the early 1900s and utilizes corridors similar to extirpated mammals. Bobcats as a focal species can serve as an indicator for habitat quality for future designated wildlife corridors. New metrics have emerged from species monitoring through remote sensing technologies such as eco-acoustic indices which summarize sound profiles, are a potential proxy for biodiversity, and could be utilized in monitoring large mammals. Here, we investigate the relationship between Lynx …

The Molecular And Behavioral Characterization Of The Murine Cre – Loxp Skeletal Muscle Synthesized Bdnf Knockout Model, Asha E. Rustad

All NMU Master's Theses

Brain derived neurotrophic factor (BDNF) is a neurotrophin essential for supporting the growth, differentiation, and health of neurons within the central and peripheral nervous systems. BDNF is also a member of the myokine family, where muscle synthesized-BDNF (msBDNF) is thought to facilitate the crucial bi-directional communication between the motor neuron and the muscle fibers it innervates. Since 2008, the Ottem Laboratory has investigated the role of msBDNF by employing Cre/LoxP recombinase technology to selectively delete the BDNF coding region within skeletal muscle of mice. This approach generates animals with either heterozygous or homozygous deletions of msBDNF, as well as BDNF …

Field Pea: Crop Management And Production, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

The field pea agronomic package available to growers is one offering simplicity and reliability. Producing high yielding field pea crops is now limited more by seasonality and rainfall rather than technology and genetics. With new varieties and agronomy package field pea is a profitable break crop option.

Expression Of Glycine Transporter 1 In The Rodent Basal Ganglia: Glial Vs. Neuronal, Rosa Atziri Perez

Open Access Theses & Dissertations

Glycine functions as an inhibitory neurotransmitter in the central nervous system (CNS),and it is mainly involved in autonomic functions. The levels of extracellular glycine are regulated by two membrane transporters, glycine transporter 1 and glycine transporter 2 (GlyT1 and GlyT2, respectively). These transporters differ in function and localization within the CNS. For many years, studies suggested that only GlyT2 served as a neuronal marker; however, GlyT1 has been localized in neurons in recent studies. The expression of GlyT1 is widespread compared to GlyT2; hence, the main objective is to determine the nature of the expression of GlyT1 in areas devoid …

Farm Management Practice For The Prevention Of Soil Erosion In The Carnarvon Horticultural Area, Kehinde O. Erinle

Horticulture research reports

The Carnarvon horticultural area has suffered considerable erosion damage following flooding of the Gascoyne River.

The purpose of this report is to describe the factors that contribute to soil erosion in the Carnarvon horticultural area (Section 1), provide guidelines for good soil management (Sections 2) and provide floodway hazard classes and associated soil management recommendations (Section 3).

This report:

• Provides detailed information on soil management for intensive irrigated cropping.
• Details cropping alternatives to better manage the soil erosion risk.
• Identifies appropriate plants for soil stabilisation in a range of situations.
• Promotes the concept of property management plans.
• Takes account of …

Tradeoffs Between Elemental Homeostasis And Growth Govern Freshwater Phytoplankton Responses To Salinization, Samuel A.T. Dias, Clay Prater, Adriana Diaz-Delgado, Natalie A. Clay, Sally A. Entrekin, Michelle A. Evans-White

Entomology and Plant Pathology Faculty Publications and Presentations

Anthropogenic salinization resulting from road salt application can degrade aquatic environments by altering the structure and function of phytoplankton communities, ultimately reducing flows of resources through aquatic food webs. However, physiological mechanisms underlying taxon-specific responses to salinization are often poorly linked to higher-order ecosystem dynamics, limiting our ability to predict community responses to salinization. To this end, we tested hypotheses derived from Subsidy-Stress and Ecological Stoichiometry theory by growing two cosmopolitan genera, Dolichospermum (prokaryotic, cyanobacteria) and Scenedesmus (eukaryotic, green algae), across NaCl gradients and contrasting differences in their growth rates, degree of Na homeostasis, and cellular C : N : …

Fouling Community Assessment On Anthropogenic Structures In Barnegat Bay, Nj: Who's Fouling With Our Lagoons?, Jesi Herterich

Theses, Dissertations and Culminating Projects

Biofouling communities consist of both mobile and non-mobile species which settle and attach themselves to both natural and artificial substrata. With increased construction of lagoon communities and marinas in Barnegat Bay, NJ utilizing PVC material, it allows space for invasive and generalist species to recruit. This study examines the impacts of man-made structures and abiotic factors on the distribution of recruiting fouling organisms in coastal lagoon communities in Barnegat Bay, NJ. Polyvinyl chloride settling plates were deployed in late May of 2024. Settling plates were retrieved and replaced at one month intervals to assess monthly settlement patterns, while a second …

The Role Of The Jnk Gene In Ovarian Cancer Inflammatory Pathways, Tashoy D. Campbell

Graduate Student Dissertations, Theses, Capstones, and Portfolios

Ovarian cancer, one of the leading causes of cancer-related deaths in women globally, presents a significant challenge due to its common late-stage diagnosis and chemoresistance (inability of the tumor to respond to drugs). The c-Jun N-terminal kinase (JNK) signaling pathway plays a crucial role in ovarian cancer progression, contributing to inflammation, tumor survival, metastasis, and chemotherapy resistance. This literature review examines the role of the JNK signaling pathway in ovarian cancer, with a focus on its role in inflammation, metastasis, and chemoresistance. JNK regulates key inflammatory cytokines, such as TNF-α and IL-6, which promote tumor growth and immune evasion. Moreover, …

Use Of Infrared Spectroscopy In Animal Studies: Aspects Of Health Science, Brian Manzanares

Graduate Student Dissertations, Theses, Capstones, and Portfolios

Non-invasive imaging technologies, such as infrared thermography (IRT), near-infrared spectroscopy (NIRS), and fourier-transform infrared spectroscopy (FTIR) are revolutionizing the field of animal research by enabling real-time, stress-free assessment of physiological conditions. Such methods like these are align with the ethical standards of the 3Rs, replacement, reduction, and refinement-by reducing the need of invasive procedures while enhancing data quality and animal welfare. These methods can also provide critical insight into animal health, behavior, and stress responses without the need for invasive procedures or restraint, thereby minimizing harm and distress. This literature review will explore the practical applications of IRT, NIRS, and …

The Role Of Nicotine In Treating Neurodegenerative Diseases, Jacquelyn T. Deaton

Graduate Student Dissertations, Theses, Capstones, and Portfolios

Neurodegenerative diseases have emerged as a critical medical challenge because they cause progressive neuronal death alongside structural or functional deterioration. Families, healthcare institutions, and affected individuals face significant challenges due to neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's diseases, which impair both cognitive function and motor abilities. Researchers have found that nicotine in tobacco products is a compound with therapeutic potential. This literature review explores how nicotine interacts with neurodegenerative mechanisms, such as oxidative stress and inflammation, and assesses its potential neuroprotective effects in conditions like Alzheimer's and Parkinson's diseases. In the central nervous system, nicotine binds to the …

Advanced Interactions Of Directed Energy With Retinal Pigment Epithelial Cells And Their Photochemical And Photothermal Responses, Jin B. Ha

Theses & Dissertations

Excess optical radiation can injure the retina through either rapid heat deposition (photothermal damage) or cumulative photo‑oxidative stress (photochemical damage), yet current laser‑safety limits treat these mechanisms as independent. To test that assumption, this dissertation integrated systematic wavelength, duration and temperature‑controlled laser exposures with live/dead fluorescence imaging in a melanin‑loaded hTERT‑RPE1 monolayer model. First, concurrent 447 nm (blue) and 2 µm (infra‑red) beams delivered for 200 s demonstrated a strong thermal–photochemical synergy: each beam alone was sub‑threshold (≤25 % lethality) but together produced 100 % RPE death without exceeding 50 °C, proving that modest heating markedly lowers the photon dose …

The Effect Of An Invasive Species (Lantana Camara) And A Fast-Growing Herb (Acanthus Pubescens) On The Plant And Animal Communities In Kibale National Park, Uganda, Kathleen Bufka

Theses and Dissertations

Fast-growing plant species, like Lantana camara and Acanthus pubescens, could threaten biodiversity in Kibale National Park, Uganda. I surveyed plants and wildlife in areas where these plants were frequently encountered compared to those areas where they are infrequent. I found mixed results based on plant traits and the animals considered. My findings support management efforts on these two species to prevent forest degradation.

Two Essays On Index-Based Risk Management In U.S. Rangeland And Livestock Production, Wajdi Belgacem

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation investigates two distinct but complementary dimensions of risk management in United States rangeland agriculture. Essay 1 measures spatial variation in basis risk within the Pasture, Rangeland, and Forage (PRF) rainfall-index program for 176 Nebraska grid cells (1988–2023). By linking official NOAA rainfall indices with 30-m Rangeland Analysis Platform biomass estimates, we find rainfall–forage correlations that peak at 0.45 but display pronounced heterogeneity. The probability that neither insured interval triggers during an actual forage loss averages 12%, rising to nearly 30% in several Sandhills cells, whereas optimally pairing late-spring and summer intervals reduces complete misses below 10%. These results …

Assessing The Impact Of Pll And Ple Concentrations And Aging On Protein Condensates Formation Via Llps, Nabil Harche

Theses, Dissertations and Culminating Projects

Protein droplets, also referred to as condensates, are membrane-less organelles formed through a biophysical process known as liquid-liquid phase separation (LLPS). These structures play an indispensable role in a wide range of cellular functions, including compartmentalizing biochemical reactions, regulating gene expression, and responding to cellular stress. Unlike traditional membrane-bound organelles, these condensates form dynamically and reversibly within the cytoplasm or nucleus, without the need for lipid membranes. The formation of these protein droplets is affected by several physicochemical factors, including pH, temperature, ionic strength, concentration of biomolecules, and specific molecular interactions such as electrostatics and hydrophobic forces. LLPS results in …

Crustose Coralline Algae Accretion In A Kelp Forest Ecosystem In Southern New England, Usa, Ruimeng Liu

Theses, Dissertations and Culminating Projects

Temperate reef ecosystems are experiencing rapid canopy loss of kelp and the proliferation of algal turf. However, the consequences of these changes for crustose coralline algae (CCA)— a key but often overlooked benthic calcifier—are poorly understood. Here, I study CCA recruitment patterns in southern New England, USA, using standardized settlement tiles at two field sites over 12 months (September 2023 – August 2024). I measure CCA accretion rates and the associated biological and environmental correlates of CCA recruitment. At the two sites, CCA accretion rates ranged from 0.0259 to 1.215 mg CaCO3 cm⁻² yr⁻¹, with an average of 0.441 mg …

Comparative Expression Of Cgmp-Dependent Protein Kinase G (Pkg) From Plasmodium And Toxoplasma, Tsopmo Asaah

Theses, Dissertations and Culminating Projects

Malaria and Toxoplasmosis are life threatening diseases to humans. The search for a cure and control of these diseases is still one of the major challenges of scientists worldwide. In this research we focused on two Malaria species; Plasmodium bergei² (Pb) and Plasmodium falciparum¹ (Pf) and also on Toxoplasma gondii. Part of the life cycle of these organisms is regulated by the enzyme cyclic GMP-dependent protein kinase G (PKG)⁴. This is our drug target in this research. We believe that inhibiting this enzyme will be a great step in fighting these diseases. In this research, we devised a comparative study …

A Pilot Study Exploring Vitamin D, Mental Health, And Gastrointestinal Health In Humans, Brianna C. Williams

Master's Theses

Mental disorders are experienced by nearly one billion people worldwide. Depression is one of the most common. Treatments exist, yet a devastating 75% of individuals suffering from depression do not receive adequate treatment. An emerging treatment strategy includes exploring the connection between mental health and nutrition, which includes the areas of gut health and nutrient deficiencies. Vitamin D is obtained through food, supplements, and sunlight. Many individuals are considered deficient (25[OH]D concentrations < 12 ng/mL) or insufficient (25[OH]D concentrations < 20 ng/mL). Purpose: This study aimed to explore mental and gastrointestinal (GI) health using surveys and lipopolysaccharide binding protein (LBP) concentrations, a biomarker of GI health, in individuals considered 25(OH)D deficient (DEF) vs not deficient (N-DEF). Methods: This cross-sectional study recruited physically active participants aged 18-45 years who underwent three evaluations in one visit. Health was assessed using surveys to evaluate mental health, GI symptoms, and vitamin D intake. Serum from fasting blood samples was used with an enzyme-linked immunosorbent assay to determine 25(OH)D and LBP concentrations. Unpaired t-tests were used to compare DEF vs N-DEF and significance was set at p < 0.05. Results: Thirty-two participants (mean age 25.3 ± 6.3 years) with mean and body fat percentage of 24.0% ± 10.7%, were placed into DEF (n=16) and N-DEF (n=16) categories. Interestingly, five of the 16 participants in DEF (31%) showed some level of depression (scores ranging from mild to severe) and only two of the 16 participants in N-DEF iv (13%) scored positively in the depression category (scores ranging from mild to moderate). Additionally, the participant with the lowest 25(OH)D concentration (5.42 ng/mL) was the only participant to meet the “severe” depression category criteria, with a score of 24 compared to other participants who scored within 0-16. Also, the only participant who scored “severe” in the stress category, was in the bottom 20% of 25(OH)D concentrations. As for GI health, the difference between average GI scores and average serum LBP concentrations between the groups were not statistically significant. Conclusion: There were no significant differences between DEF and N-DEF in perceived depression (p = 0.16), anxiety (p = 1.0), stress (p = 0.96), GI symptoms (P values ranged from 0.27 to 1.00), nor in LBP concentrations (p = 0.77). Funding: Support was received from the Graduate Student Association and College of Natural Health Sciences at the University of Northern Colorado.

Effects Of Chronic Marijuana Use On Postural Control During Quiet Standing, Johnathon S. Ashcraft

Master's Theses

Marijuana, Cannabis Sativa, is the most commonly used recreational drug in the United States, with more than 60 million people reporting use in 2022 (Substance Abuse and Mental Health Services Administration, 2023). As marijuana consumption has increased, so have questions surrounding its acute and long-term effects. Its acute effects are well documented; however, the long-term effects are not as well understood. It is hypothesized that chronic marijuana users will exhibit a decrease in postural stability during quiet standing when compared to non-users, so the purpose of this study was to compare postural control of chronic marijuana users and non-users in …

Nebline, August/September 2025

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Contents

Summertime’s Natural Light Show: Fireflies by Kait Chapman

Insect Science with Kids: Bug Collecting as a Tool to Learn

The Perfect Partnership: EFNEP and SNAP-Ed Join Forces for Lifelong Nutrition and Health by Emily Gratopp

Recipe of the month by Emily Gratopp: Fruit salsa & cinnamon chips

Southeast nebraska Pasture & Acreage Expo

Southeast Nebraska Alfalfa & Wheat Expo

Landlord/Tenant Cash Rent workshop

Vegetable Gardening in the Fall by Mary Jane Frogge

GRO Big Red virtual learning series

2025 perennial plant of the year: Blue mountain mint (Pycnanthemum muticum)

Garden guide: Things to do this month by …

Navigating Transportation Barriers: Older Adults’ Familiarity With New Mobility Options And Perceptions Toward Autonomous Vehicles In Arkansas, Arna Nishita Nithila, Suman Kumar Mitra, Michelle Gray, Alishia Juanelle Ferguson, Jennifer D. Webb

Civil Engineering Faculty Publications and Presentations

The objective of this study is to analyze older adults’ familiarity with new transportation options (ride-hailing services, bike-share services, and shared e-scooter services) and their perception towards autonomous vehicles (fully autonomous cars), as well as how transportation barriers influence their familiarity and perceptions, in Arkansas, a predominantly rural state. Data from 775 older adults aged 60 years or older were collected between October 2021 and October 2022. To fulfill the study objective, the study used Latent Class Cluster Analysis to segment older adults into classes based on their familiarity with new transportation options and their perceptions of autonomous vehicles. The …

Optimizing Delphinium And Larkspur Through Timing And Protective Cultivation Methods For Cut Flower Production, Nicholas Kirwan Volesky

All Graduate Theses and Dissertations, Fall 2023 to Present

Cut flowers have become an increasingly important specialty crop for small farms nationwide, and particularly in Utah, where decreasing agricultural land has heightened the need for profitable cropping options. However, production is challenged by Utah’s high-elevation climate, which presents unique constraints not addressed by current recommendations. Cool-season crops, like delphinium (Delphinium spp.) and larkspur (Consolida ajacis L.), are underproduced yet highly marketable, generating strong interest among growers throughout the state. Developing region-specific production guidelines is essential to help optimize yields, extend harvest windows, and meet local demand. This research evaluated spring planting dates in high tunnel and field …