Life Sciences Commons^™

Determining The Role Of Maumee River Sediments In Microbial Nitrogen Transformations And Net Fluxes, Ashlyn Stanalonis

Master's Theses

The Maumee River watershed is the largest of any river feeding into the Laurentian Great Lakes and is primarily comprised of agricultural land uses. Most cultivated crops require fertilizer applications to sustain growth and productivity. Fertilizer runoff is a major source of nutrient loading to the river, and total nitrogen (N) and phosphorus (P) inputs contribute to eutrophication and cyanobacterial blooms in the western basin of Lake Erie. This study aimed to characterize Maumee River sediments as net N sinks or sources to the western basin and evaluate N removal efficiency (a valuable ecosystem service) to determine if river sediments …

Blue Crab Reproductive Output And Phenology In A Changing Climate, Blaise Rohan

Master's Theses

Globally, ecosystems are experiencing rising temperatures due to climate change, with potential consequences for reproductive investment and timing in many species. Blue crabs, Callinectes sapidus, support valuable commercial fisheries throughout the U.S. Atlantic and Gulf coasts, and understanding variation in reproductive output has important management and conservation implications. This study examined two components of blue crab reproduction: (1) reproductive investment across populations spanning multiple latitudes (New York, New Jersey, North Carolina, Mississippi, and Texas), using a space-for-time substitution to assess the effects of varying thermal regimes on fecundity and egg quality, and (2) spawning phenology in North Carolina from …

Concerns With The Statistical Analysis In “Effect Of Combined Glp-1 Analogue And Bupropion/Naltrexone On Weight Loss: A Retrospective Cohort Study”, Thirupathi Reddy Mokalla, Henry T Robertson, Sherri Pals, David B Allison

Children’s Nutrition Research Center Staff Publications

No abstract provided.

All-Cause And Cause-Specific Mortality In Gambling Disorder: Evidence From A Nation-Wide Matched And Sibling Cohort Study In Taiwan, Yang-Chieh Brian Chen, Chih-Wei Hsu, Liang-Jen Wang, Mu-Hong Chen, Yao-Hsu Yang, Chih-Sung Liang, Edward Chia-Cheng Lai

Children’s Nutrition Research Center Staff Publications

Background and aims: Gambling disorder (GD) has been linked to suicidal ideation and suicide deaths; however, evidence on all-cause and cause-specific mortality-particularly in Asian populations-remains limited. Using a retrospective cohort study based on nationwide matched and sibling cohort, we investigated all-cause and cause-specific mortality risk in patients with GD.

Design, setting and participants: A retrospective cohort study was conducted using 2000-2022 data from Taiwan's National Health Insurance Research Database. A cohort of 961 individuals diagnosed with GD was identified. An age- and sex-matched control cohort (n = 3,844) and an unaffected sibling cohort (n = 675) were constructed. Cumulative survival …

Hyperadhesive Von Willebrand Factor Contributes To Pathogenesis Of Preeclampsia, Yajuan Wang, Chenyu Wang, Katie L Houck, Xiaoli Gao, Yuanyuan Chen, Xin Xu, Xue Zhao, Miguel A Cruz, Shu Zhang, Chester Q Li, Fengxia Xue, Min Li, Swati Shree, Jing-Fei Dong, Cha Han

Children’s Nutrition Research Center Staff Publications

Background: Preeclampsia is the most common complication of pregnancy, significantly affecting maternal and fetal health, and is characterized by placental and systemic endotheliopathy. Patients with preeclampsia have elevated levels of VWF (von Willebrand Factor), which is associated with poor clinical outcomes. However, whether VWF serves as a marker for endotheliopathy or contributes to the pathogenesis of preeclampsia remains poorly understood.

Methods: We investigated the role of hyperadhesive VWF in the development of preeclampsia by studying patients, evaluating mouse models, and performing in vitro experiments.

Results: We show that patients develop VWF- and fibrin-rich thrombosis in the placenta and have significantly …

Full-Fat Dairy Milk Vs. Isolated Free Nutrient Blend On Postprandial Protein Metabolism And Inflammatory Markers In Adults With Overweight And Obesity, Takeshi M. Barnes, Zan Zupancic, Max T. Deutz, Andrew T. Askow, Colleen F. Mckenna, Jonathan A. Aguilera, Cassidy T. Tinline-Goodfellow, Alexander V. Ulanov, Jared W. Willard, Daniel W. D. West, Michael De Lisio, Daniel R. Moore, Nicholas A. Burd

Kinesiology & Sport Management Scholarship

Excess adiposity is linked to anabolic resistance by blunting the postprandial myofibrillar protein synthetic response to protein ingestion. Full-fat dairy milk may overcome this resistance via a matrix combining high-quality protein with anti-inflammatory lipids. Therefore, we compared the effects of ingesting full-fat dairy milk versus a non-dairy macronutrient match isolated blend on the regulation of postprandial myofibrillar and mitochondrial protein synthesis rates and markers of systemic (i.e., IL-6 and TNF-α) and muscle-related inflammation (i.e., NF-κB, NLRP3, TLR4, and MyD88). Twenty-two obese and overweight adult males and females (BMI = 31.1 ± 3.7 kg m−2; aged 35.3 ± 13.2 y) were …

Genetic Regulation Of Uterine Organogenesis, Diana A. Machado

Dissertations and Theses (Open Access)

In eutherian and marsupial mammals, the site of embryo implantation and gestation is the uterus. The precursor tissue of the uterus, oviducts, cervix, and upper vagina is the Müllerian duct epithelium and its adjacent mesenchyme. Morphological uterine variation between species is established during embryogenesis by species-specific differences in Müllerian duct fusion at the midline, growth, and differentiation. For example, laboratory mice have a bipartite uterus with two uterine horns and a single cervix, whereas humans have a simplex uterus with a single chamber and single cervix. In humans, variations in the fusion process can alter typical uterine morphology, correlating with …

Economic Contribution Of The Agricultural Sector To The Arkansas Economy In 2024, Frank Seo, Luke Pullo

AAES Research Reports and Research Bulletins

This report is the nineteenth in a series that examines agriculture’s economic contribution to the Arkansas economy. The total economic contribution of agriculture (direct, indirect, and induced effects) on value added, employment, and labor income was estimated with the Impact Analysis for Planning System (IMPLAN) for the most recent year available, 2024.

Paleolimnology And Diatom Taxonomy From Lake Tanganyika, Sristika Adhikari

All-Inclusive List of Electronic Theses and Dissertations

Lake Tanganyika is one of the world's largest freshwater ecosystems, supporting rich aquatic biodiversity and providing several ecosystem services to surrounding communities. Environmental changes such as rising lake surface temperatures, pollution, and land-use alterations threaten the lake’s sustainability. This research examines spatial and temporal variations in nutrient dynamics and primary productivity of Lake Tanganyika using diatom assemblages from multiple locations. Our findings indicate reduced nutrient levels and longer stratification periods in the lake’s central basin during most of the Common Era, as indicated by a remarkable increase in the relative abundance of long, needle-shaped planktonic Nitzschia species. In the southern …

Evaluating The Motivations Of Participants In Online Equine Extension Courses, Sarah Vos

Department of Animal Science: Dissertations, Theses, and Student Research

Traditional program evaluations emphasize participant demographics, knowledge gain, behavior change, and program impact. However, evaluating participant motivation can provide addition insight into why participants enroll in extension programs and help explain participant engagement and persistence throughout a program. The purpose of this study was to evaluate participant motivation in two online equine extension courses and examine potential relationships between motivation, demographics, and course delivery method, and describe participant engagement in Virtual Horse Judging Camp (VHJC). This cross-sectional study utilized convenience sampling of participants in two online equine extension courses, Horse Adult Leader Training and Educational Resource (HALTER) and VHJC. The …

Fibrous Protein Biomaterials: Characterization Of Solitary Bee Silk And Cocoons, And Development Of Recombinant Hagfish And Bee Silk Materials, Oran Wasserman

All Graduate Theses and Dissertations, Fall 2023 to Present

This dissertation explores two complementary aspects of fibrous protein biomaterials: the characterization of understudied naturally occurring solitary bee silk and cocoons; and recombinant expression and biomaterial formation of recombinant hagfish intermediate filaments (rHIF) and a single Osmia lignaria Say (Hymenoptera: Megachilidae) silk protein. Some solitary bee species, which make up about 75% of all bee species, spin silk to construct protective cocoons during development. Despite the importance of silk and cocoons to the solitary bees' life history, their properties remain largely unexplored. This dissertation presents a novel method for isolating and testing individual silk fibers without interfering with the development …

Investigating Recruitment Bottlenecks Of Juvenile Native Fish In The Colorado River Basin, Utah, Anna R. Amidon

All Graduate Theses and Dissertations, Fall 2023 to Present

The first year of life is a vulnerable period for most fish species. Whether young fish survive this early stage determines how many individuals eventually survive and grow into reproductive adults, a process called recruitment. In desert rivers like the Colorado River Basin, native fish evolved alongside extreme natural conditions but now face additional pressure from drought and water storage infrastructure, which alter the shoreline habitats on which young fish depend. Long-lived, migratory native species with complex life cycles are especially vulnerable. We used four decades of monitoring data from the Upper Colorado River Basin to evaluate whether young-of-year (YOY, …

The Influence Of Habitat And Individual Specialization On Mountain Lion (Puma Concolor) Hunting Strategy In A Multi-Prey System, Hannah B. Klugman

All Graduate Theses and Dissertations, Fall 2023 to Present

There is a growing recognition that animal species considered generalists (having a broad and varied diet), are actually specialists, a collection of individuals with different preferences and strategies known as specializations. The diet of a specialist depends on the variety and quantity of available food options, and their own ability to take what they need. Mountain lions (Puma concolor) are known for their ability and tendency to hunt large prey, such as elk (Cervus canadensis) and mule deer (Odocoileus hemionus), but despite the fact they overlap with other potential large prey, like feral horses …

Drought And Diet Breadth: Does Insect Specialization Influence Herbivory When Plants Are Under Stress?, Jakob Palmer

All Graduate Theses and Dissertations, Fall 2023 to Present

Climate change will alter precipitation worldwide, causing more frequent and severe drought events worldwide. Drought affects many ecological processes, including plant-herbivore interactions, which are critical to biodiversity and ecosystem health. A variety of plant characteristics can influence herbivory – such as how hairy, nutritious, or toxic a leaf is – and drought has been shown to alter many of these traits. Unique characteristics of herbivore species are also important: herbivores can either feed on a variety of plants (generalists) or a group of closely related plants (specialists). Specialists can cope with traits of the plants they eat, while generalists are …

Behavioral And Landscape Factors Affecting The Effectiveness Of Egg-Bait Management For Common Ravens In The Great Basin, Usa, Sierra L. Pederson

All Graduate Theses and Dissertations, Fall 2023 to Present

Common Ravens have become increasingly common across the Great Basin of the western USA, largely because human activities, such as roads, agriculture, livestock operations, and human-made structures, provide them with abundant food, water, and nesting sites. As raven numbers have grown, so has their impact on vulnerable species like the greater sage-grouse. Like many other ground-nesting species, greater sage-grouse eggs and chicks are especially susceptible to predation. To help reduce raven numbers in sensitive areas, wildlife managers often place hard-boiled chicken eggs treated with a toxin (DRC-1339) on the landscape, expecting that ravens will die after eating these eggs and …

Analysis Of The Cannabinoid Compositions, Byproducts And Heavy Metals In Unregulated Cannabinoid Products By Hplc-Ms/Icp-Ms, Simone K. Melle

All NMU Master's Theses

The rapid expansion of unregulated hemp-derived products has created a need for analytical methods that characterize both cannabinoid composition and contaminant occurrence. This thesis used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to quantify cannabidiol (CBD), Δ8-tetrahydrocannabinol (Δ8-THC), and Δ9-tetrahydrocannabinol (Δ9-THC), and inductively coupled plasma mass spectrometry (ICP-MS) to measure Al, As, Cd, Cr, Cu, Fe, Mg, Ni, Pb, and Zn in commercial hemp-derived products, laboratory-prepared hemp oils, cultivated hemp tissues, extracted material, and post-harvest soil. Commercial products showed wide cannabinoid variability, with several samples containing more than one target cannabinoid, while laboratory-prepared plant oils showed a more consistent profile dominated by …

Melatonin-Functionalized Silver Nanoparticles Enhance Salinity Tolerance In Brassica Juncea By Modulating Antioxidant Defence And Growth Physiology, Mohini Yadav, Manar Fawzi Bani Mfarrej, Vikash Kumar, Ankita Singh, Faheem Ahmad, Saurabh C. Saxena

All Works

Soil salinity is a major abiotic stress that severely limits plant growth and productivity by inducing osmotic stress, oxidative damage, and ionic imbalances. The present study evaluated the potential of melatonin-mediated silver nanoparticles (Mel AgNPs) to enhance salinity stress tolerance in Brassica juncea. Pot experiments were conducted using 21-day-old plants exposed to salinity stress and treated with Mel AgNPs at different concentrations (50 and 100 mg L⁻¹) through both root application and foliar spray. Morphological, physiological, and biochemical responses were assessed 48 h after nanoparticle exposure. Salinity stress markedly increased lipid peroxidation and hydrogen peroxide accumulation while suppressing antioxidant defence. …

Validated Crispr/Cas9 Guide Rnas Targeting Neurodevelopmental Genes In The Tunicate Ciona Robusta, Sydney Popsuj, Tenzin Kalsang, Eduardo D. Gigante

Faculty Articles

The tunicate Ciona robusta provides a powerful and simplified model for dissecting the genetic control of developmental and cell biology. With a larval CNS composed of just over 200 neurons and sensory cells, it has also emerged as a model organism for neurobiology and the development of the nervous system. Although CRISPR/ Cas9-mediated mutagenesis is now routinely used in Ciona as an important technique used to interrogate gene function in diverse biological processes, validated single-guide RNAs (sgRNAs) have yet to be validated for several key neural genes. Here, we report the design and experimental validation of 25 novel sgRNAs targeting …

Eusocial Caste Specialization Under Abiotic And Biotic Stress In A Stingless Bee, Tetragonisca Angustula, Kristin M. Robinson

Dissertations

Eusocial insects rely on division of labor to achieve high colony efficiency and provide essential ecosystem services, but specialization may also constrain colony resilience when workers operate near their physiological or behavioral limits under abiotic and biotic stressors. How specialization mediates these responses remains poorly understood, particularly in stingless bees (Tribe: Meliponini). Using the agriculturally relevant stingless bee Tetragonisca angustula, which possesses a specialized soldier subcaste, I investigated how a high degree of worker specialization influences physiological tolerance and predator responses across worker subcastes.

In this dissertation, I quantified variation in thermal tolerance and desiccation resistance among worker subcastes …

History-Dependent Gain Control In The Paraventricular Thalamus: Neural Substrates Of Stress-Enhanced Fear Learning, Michael Martino

MUSC Theses and Dissertations

Post-traumatic stress disorder (PTSD) is characterized by hyperarousal and heightened reactivity that generalize beyond the original traumatic event, yet the neural mechanisms by which prior stress experience biases future fear learning remain poorly understood. Stress-enhanced fear learning (SEFL) is a well-validated rodent model of this phenomenon, in which a severe stressor produces persistent sensitization of subsequent fear acquisition. This dissertation used a 30-day SEFL paradigm to identify the brain-wide functional signature of stress sensitization and to identify candidate neural substrates.

Whole-brain cFos mapping during acute fear acquisition revealed a predominant pattern of suppressed neural activity in sensitized animals, with limbic …

Effects Of Beverage Color During Mouth Rinsing On Measures Of Muscular Endurance, Muscular Strength, And Exercise Perceptions During Isometric Handgrip Testing, Sydney Andrews

Exercise Science Theses & Dissertations

Previous research has shown that color can influence psychological responses, physiological responses, and exercise performance. These effects have been previously attributed to learned associations or expectations which may influence exercise-related outcomes. This study aimed to investigate whether beverage color influences muscular performance during intermittent isometric handgrip exercise. This randomized, single-blind, deceptive crossover study consisted of 34 physically active adults (21.9±3.1 years) who completed a handgrip endurance test under three conditions: red beverage rinse, clear beverage rinse, and no-rinse. Participants were informed that the study would examine the effects of mouth rinsing two commercially available carbohydrate containing sport drinks on muscular …

Long-Term Spatiotemporal Change In Coral Reef Fish Populations And Communities In The Philippines, John Christopher Whalen

Biological Sciences Theses & Dissertations

The Visayas Region of the Philippines is a global epicenter of marine fish biodiversity. Hypotheses attribute this peak of species richness within the Coral Triangle to processes that occur at geological time scales. However, changes in biodiversity may occur on ecological time scales, allowing for the assessment of the potential impact of anthropogenic activities. Recent declines in genetic, species, and ecosystem diversity have been observed in the tropics, including the Philippines. Within the Philippines the Visayas Region is considered the epicenter of species adversity due to anthropogenic stressors such as habitat degradation, overfishing, and harvesting individuals for the aquarium trade. …

Computational And Ai Tools For Understanding Telomere-Associated Cancer Mechanisms, Eleni Adam

Computer Science Theses & Dissertations

Telomeres are the protective caps of the human chromosomes and are critical for genome stability. Dysfunctional telomeres caused by their erosion with age and cell proliferation as well as by defects in their maintenance is a major early event leading to genome changes and cancer. Subtelomeres possess the critical role of regulating adjacent telomeres. Due to their complex repeat structure and high variance from one person to another, these areas have not been analyzed in detail. We present a set of computational and machine learning tools to aid in the understanding of subtelomere structure and its rearrangements in cancer.

Initially, …

A Geometric Morphometric Analysis Of Acetabular Shape Variation Across Bat Families, Jordan L. Huff

Electronic Theses and Dissertations

Bats are the only mammals capable of powered flight, and research is concentrated on this adaptation. Their non-flight locomotion has received comparatively little attention. Previous studies show that non-flight locomotor behaviors vary across families. Members of Pteropodidae are efficient climbers but cannot effectively walk, whereas Phyllostomidae and Vespertilionidae are better at terrestrial locomotion but show reduced arboreal capabilities. The non-flight locomotion of Hipposideridae and Rhinolophidae is less understood, although rhinolophids exhibit limited terrestrial locomotion. This study examined whether differences in locomotor behavior are associated with variation in acetabular morphology. A geometric morphometric analysis of acetabular shape was conducted across multiple …

Testing P300-Based Biometric Authentication Paradigms, Jordan Razzak

Electronic Theses and Dissertations

Bench research suggests that P300 amplitude is greater in response to self-relevant stimuli compared to familiar stimuli. This effect could enable a novel method of biometric authentication based on the self-relevant P300. This dissertation aims to: (1) determine the optimal presentation paradigm for leveraging self-relevance in P300-based biometric authentication, and (2) investigate P300 responses from self-relevant stimuli versus stimuli learned over the course of a week.

Two presentation paradigms were compared – rapid serial visual presentation (RSVP) and matrix presentation (MP). Participant-specific RSVP and MP classifiers were trained by applying stepwise linear discriminant analysis to data collected during presentations of …

Mechanism Of Hypothiocyanous Acid Reductase, Morgan L. Fowler

Masters Theses

Hypothiocyanous acid reductase (Har) is a flavin-dependent enzyme that is effective in protecting bacteria from hypothiocyanous acid. HOSCN is an antimicrobial oxidant that is excreted by the mammalian innate immune system during inflammation, targeting and attacking thiols in pathogens. To combat HOSCN, bacteria have developed defense systems, including hypothiocyanous acid reductase. The catalytic mechanism by which Har interacts with its substrates, HOSCN and NADH, is undetermined and requires exploration.

In this study, we characterized the reductive and oxidative half-reactions of E. coli Har (EcHar). We first investigated and characterized wild-type EcHar, showing that Har is extremely efficient in reacting with …

The Evolution Of Hymenopteran Recombination Rates, Timothy John Delory

All Graduate Theses and Dissertations, Fall 2023 to Present

Individuals of some species mix their DNA frequently when producing sex cells, while others do so much less. Hymenopterans (ants bees and wasps) are especially useful for understanding this variation for several reasons. They show large differences in this frequency of shuffling, or rate of recombination, have well-studied genomes, and vary widely in how they live and reproduce. I capitalized on these features examines how recombination rates have evolved in this group at the trait, and gene level.

First, I revisited long-standing ideas about why recombination rates differ among these species, incorporating more than a decade of new research. This …

Divergent Belowground Strategies Of Savanna Grasses Under Varying Fire Regimes, Abigail C. Schmidt

All Graduate Theses and Dissertations, Fall 2023 to Present

Savannas are unique ecosystems, where trees and grasses live together across the landscape, not quite forming full forests or open grasslands. The ability for trees and grass to coexist is largely the result of widespread disturbances, such as fire. Fire in savannas is largely thought of in how it maintains plants above ground, however, fire also plays a key role in ecosystem and plant processes belowground. First, fire can change how soil nutrients, namely phosphorus, are cycled. Phosphorus is a key nutrient that plants need to function, and plants acquire phosphorus through their roots. Frequent fire can enhance phosphorus availability, …

Secure Agricultural Futures: Re-Defining And -Imagining Agricultural Water Landscapes In The Us West, Britta L. Schumacher

All Graduate Theses and Dissertations, Fall 2023 to Present

Aridity is swiftly becoming the US West’s new normal. As agricultural communities adjust to persistent growing-season scarcity, they find their livelihoods under increasing threat. This points to the urgency of finding paths toward more secure water futures. A piece of this future puzzle includes water (re-)distribution – taking excess from one place and delivering it to another in need. To begin thinking about (re-)distribution, this dissertation engages with three mixed-methods and multiscale studies. The first study pairs county-level data across the 11 westernmost US states with survey data collected from individual producers in Utah to assess past and current trends …

Bees In The Trap: Leveraging Bumble Bee (Hymenoptera: Apidae: Bombus) Bycatch For Ecological And Genetic Insights, Tori L. Strausser

All Graduate Theses and Dissertations, Fall 2023 to Present

Managers rely on insect monitoring traps to detect insect pests that harm crops and protect food and other agricultural production. However, these traps also capture non-target insects, including bumble bees, raising concerns about whether repeated trapping could harm pollinator populations. This thesis explores whether bees caught as non-targets, also called bycatch, in routine pest monitoring traps can be used to better understand bumble bee populations, and whether traps pose risks to their long-term persistence. Using bumble bees collected from pest surveillance traps placed in crop fields across Utah, this research examined seasonal patterns in body size, timing of capture, and …