Life Sciences Commons^™

Preclinical Models Of Melanoma Leptomeningeal Disease To Assess Intrathecal Checkpoint Blockade, Renato A Guerrieri, Grant M Fischer, Jacob R Cortez, Barbara G Knighton, Debora A Ledesma, Courtney W Hudgens, Yimmy F Delcid, Qianghua Hu, Christian Y B Onana, Fernando C L Carapeto, Michael T Tezlaff, Jason T Huse, Patrick Hwu, Elizabeth M Burton, Isabella C Glitza Oliva, Michael A Davies, Sherise D Ferguson

Faculty, Staff and Student Publications

Leptomeningeal disease (LMD) is a subtype of central nervous system metastatic disease that is associated with poor patient outcomes and limited treatment options. There is an unmet need to develop preclinical models of LMD to expedite and improve the development of new therapeutics. Here, we describe the development of multiple orthotopic immunocompetent murine models of melanoma LMD, including their use to assess the efficacy of systemic and/or intrathecal immunotherapy. LMD was established by direct intrathecal injection of murine cell lines (B16-F10, BP, D4M, D4M-UV2, MC38-gp100, RMS, YUMM3.1, and YUMMER1.7) into the cisterna magna of C57BL/6 mice. Tumor take rate, distribution, …

Long-Term Outcomes In Flt3-Mutated Acute Myeloid Leukemia After Frontline Hypomethylating Agent, Venetoclax And A Flt3 Inhibitor, Nicholas J Short, Sanam Loghavi, Musa Yilmaz, Omer Karrar, Kunhwa Kim, Courtney D Dinardo, Tapan M Kadia, Manuel Maroun, Gautam Borthakur, Ghayas C Issa, Joseph Jabbour, Betul Oran, Elizabeth J Shpall, Uday Popat, Keyur P Patel, Mark Routbort, Marina Konopleva, Farhad Ravandi, Hagop Kantarjian, Naval Daver

Faculty, Staff and Student Publications

Triplet regimens with a hypomethylating agent, venetoclax and a FLT3 inhibitor yield high rates of response in newly diagnosed FLT3-mutated AML. However, the long-term outcomes and patterns of relapse with these triplet regimens are not well-established. In this retrospective analysis, 73 patients with newly diagnosed FLT3-mutated AML received a frontline FLT3 inhibitor-containing triplet regimen. The composite complete remission (CR) and CR with incomplete hematologic recovery (CRi) rate was 93%. Next-generation sequencing FLT3-ITD MRD negativity (sensitivity: 0.005%) was achieved in 60% of patients after cycle 2 and 90% after cycle 4. The estimated 3-year relapse-free survival (RFS) for FLT3-ITD-mutated and FLT3 …

Reversible Arginine Methylation Of Pi3kc2Α Controls Mitotic Spindle Dynamics, Yena Cho, Jee Won Hwang, Mark T Bedford, Dong Hee Na, Dae-Geun Song, Su-Nam Kim, Yong Kee Kim

Faculty, Staff and Student Publications

Microtubules, composed of αβ-tubulin dimers, undergo dynamic polymerization and are fundamental to cell structure and function. In the current study, we discovered that phosphatidylinositol 3-kinase class 2α (PI3KC2α) acts as a novel regulatory factor in microtubule dynamics. Specifically, asymmetric dimethylation of PI3KC2α at the R175 residue (R175me2a) by coactivator-associated arginine methyltransferase 1 (CARM1) enhances its interaction with α-tubulin, stabilizing microtubule assembly. Furthermore, lysine Demethylase 4 A (KDM4A) serves as an arginine demethylase for PI3KC2α R175me2a. During mitosis, protein kinase C (PKC)-mediated phosphorylation of KDM4A results in its dissociation from PI3KC2α, preventing demethylation and increasing R175me2a levels. This facilitates spindle formation …

Attributes Of Artificial Wetlands Colonized By Ambystoma Maculatum On A Virginia Surface Mine, Hunter Hill, Garrett Stiltner, Walter H. Smith

Virginia Journal of Science

Landscapes impacted by surface mineral extraction present a range of artificial wetland habitats potentially useable by pool-breeding amphibians, although little information exists in the literature detailing which attributes influence breeding site selection by wetland amphibian specialists in post-mined landscapes. We compared attributes of artificial wetlands consistently used and unused as oviposition sites by Spotted Salamanders (Ambystoma maculatum) over a six-year period on a 120-ha former surface mine in Wise County, Virginia, USA. Salamanders non-randomly selected wetlands as oviposition sites, with one-third of available wetlands within the study area showing evidence of ovipositioning. Wetlands used as breeding sites were …

How To Identify Leafhoppers, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity factsheets

These tiny plant-feeding insects are often considered pests due to the damage they can inflict on crops and gardens. Leafhoppers, treehoppers and spittlebugs (or froghoppers) are closely related to cicadas, and planthoppers are their more distant relatives. As their names suggest, most of them can hop.

Attitudes Toward The Continued Protection Of Gray Wolves Under The Endangered Species Act, John A. Vucetich, Jeremy T. Bruskotter

Michigan Tech Publications

In February 2024, the U.S. Fish & Wildlife Service announced their intention to develop – for the first time – a National Recovery Plan under the Endangered Species Act (ESA) for gray wolves in the lower 48 states. In August 2025, a federal judge vacated the U.S. Fish and Wildlife Service's (FWS) decision denying ESA protections for gray wolves in the Western U.S.

Those events represent key context for this report, which describes the results of a web-based survey implemented in July and August of 2025 and designed to assess the attitudes of residents of the contiguous United States, as …

The Effects Of Dietary Protein Source On Spine Regeneration In The Sea Urchin Lytechinus Variegatus, Joseph Cortez, Jami De Jesus, Raven A. Edwards, Jeri L. Brandom, Stephen A. Watts

Expo Student Presentations

2025 Fall Expo Poster Presentation

• Biological & Life Sciences

Endocrine Gene Divergence Reflects Precipitation-Driven Adaptation In Sonoran Desert Rattlesnakes, Makinley Ross Kendrick, Austin Biddy, Greer Dolby

Expo Student Presentations

2025 Fall Expo Poster Presentation

• Biological & Life Sciences

Recruitment Progress And Participant Characteristics In The Glo Study, Andre Odom

Expo Student Presentations

2025 Fall Expo Online Oral Presentation

• Biological & Life Sciences

Aav-Progranulin Gene Therapy Corrects Phenotypes Associated With Progranulin Deficiency Up To One-Year Post Injection In Mouse Model Of Frontotemporal Dementia, Hesham Shiekhtholth, Stephanie Fox, Mary Bullock, Erik Roberson

Expo Student Presentations

2025 Fall Expo Oral Presentation

• Biological & Life Sciences

Fisheries Research Report No. 354: Recreational Fishing For Abalone In Western Australia In 2024/2025 - Estimates Of Participation, Effort And Catch, Claire B. Smallwood, Karina L. Ryan, Lachlan W. Strain

Fisheries Research Reports

The Western Australian Recreational Abalone Fishery (RAF) operates in shallow coastal waters as a hand collection fishery while wading or diving. Three species of abalone are allowed to be caught. Roe’s abalone (Haliotis roei) are generally collected while wading from the shore in accessible nearshore reef complexes along the West and South Coast Bioregions. Greenlip abalone (H. laevigata) and Brownlip abalone (H. conicopora) are collected while wading (including snorkelling and freediving) and diving (using compressed air) predominantly along the South Coast Bioregion.

Recreational fishing for the three abalone species is licensed and highly regulated, especially in the Western Zone (which …

Nebline, October 2025

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Contents

4-H super fair helps youth become beyond ready

What's popping? by Natalie Sehi

Recipes of the month, by Kayla Colgrove: Fish bait snack mix, kicked up popcorn, and cinnamon crunch snack mix

Are your trees for the next storm? by Rachel Anderson

Garden guide: Things to do this month by Mary Jane Frogge

GRO Big Red virtual learning series

Composting demonstration

Promoting language development in young children by Hayley Jackson

Tip sheet for harvest 2025 by John Nelson

Rogers Memorial Day farm field day, September 2025

Heart of 4-H volunteer award: Rachel Wells

Join 4-H to become beyond ready …

West Coast Demersal Scalefish Resource Recovery Support Package: 2024-25 Report, Department Of Primary Industries And Regional Development, Western Australia

Fisheries Published Reports

The West Coast Demersal Scalefish Resource (WCDSR) includes over 100 species in inshore (20–250 m deep) and offshore ( > 250 m) demersal habitats of the West Coast Bioregion. These species are caught by charter, commercial and recreational fishers. The WCDSR is in recovery and managed under a 20-year recovery plan (2010–2030). DPIRD tracks recovery progress through scientific stock assessments of indicator species (snapper, WA dhufish, and baldchin groper) every 3 years. The 2021 WCDSR stock assessment showed limited recovery and additional management action was required. In December 2022, the Minister for Fisheries announced commercial and recreational (including charter) sector management …

Search, The Jackson Laboratory

Search Magazine

No abstract provided.

Designing Portugal’S Voluntary Carbon Market: Insights From Key Stakeholders, Mahika Shergill

Portugal: Sustainability and Environmental Justice

This project examines the design, integrity, and early implementation challenges of Portugal’s newly established Voluntary Carbon Market (VCM), created through Decree-Law 4/2024 as part of the country’s broader climate-mitigation strategy. The objective of the study is to evaluate whether the market’s regulatory architecture—particularly its treatment of additionality, permanence, methodologies, and co-benefit mechanisms—positions it to function as a credible and viable national carbon-governance instrument. The analysis draws on a mixed-methods approach combining legal and policy review with four semi-structured interviews conducted with policymakers, methodological designers, ecological practitioners, and environmental-law specialists. These interviews reveal that Portugal’s VCM is characterized by strong integrity …

Motives And Community Energy: What Determines Participation In The U.S. And E.U., Belinda Litchtenfeld

Portugal: Sustainability and Environmental Justice

The shift to renewable energies has become a critical means of addressing the climate crisis. Community Energy (CE) has emerged as a ‘bottom-up’ transition to achieve decarbonization within a localized environment. The aim of this paper is to compare the motivations and framings of rural CE projects in the United States and European Union. The paper will compare the case of Loess Wind Farm, located in Rock Port, Missouri, with the Cedillo Solar Community in Caceres, Spain to determine how sociotechnical visions are shaped by the challenges facing communities. The elements of sociotechnical visions- meanings, knowings, doings, and organizings- as …

When The Water Runs Out: Modelling Portuguese Pumped Hydro Storage Adequacy In Multi-Year Extreme Drought Conditions, Meg Gillies

Portugal: Sustainability and Environmental Justice

Portugal’s reliance on Pumped Hydro Storage (PHS) to support grid stability poses increasing risks as the share of renewables in the national energy mix approaches the 2030 goal of 85%, and droughts intensify across the Iberian Peninsula (Silva, 2024). Because simulation tools like EnergyPLAN model only one year at a time, they overlook the cumulative effects of prolonged hydrological stress (Lund, 2024). This research addresses that gap by implementing an iterative multi-year approach in EnergyPLAN, linking yearly simulations to assess how extended drought conditions impact PHS performance. Using a detailed 2024 model of Portugal’s power system and applying dry year …

Save The Bees And The Land: A Framework To Connect The Wildfires In Portugal To The Change In Bee Populations, Lacy Creswell

Portugal: Sustainability and Environmental Justice

Wildfire is an ecological process that is important to ecosystems around the world. Fire is naturally occurring and is also used as a forest management technique to promote biodiversity. While previous studies have analyzed how wildfires affect insects, none of the studies have been based in Portugal about native bees or insects. Additionally, previous studies have addressed the impact wildfire has on land and forests; however, the gap in knowledge is how wildfire directly influences native bee species’ populations. This study assesses how wildfires in Portugal impact the native bee populations and how the lack of pollen and nectar post-fire, …

Urban Green Spaces, Sustainability Discourse, And Environmental Inequality In Agadir, Morocco, Julia Card

Morocco: Human Rights, Social Justice, and Cultural Transformation

As Moroccan cities experience rising temperatures, increased drought, and rapid urban development, urban green spaces have become central to sustainability and climate adaptation agendas. However, the social, ecological, and political implications of urban greening remain unevenly examined in semi-arid contexts. This paper investigates how green spaces are envisioned, implemented, and experienced in Agadir, a coastal city shaped by colonial planning legacies, post-earthquake reconstruction, water scarcity, and event-driven redevelopment. Using a qualitative methodology combining a visual content analysis of the 2022–2027 Agadir Communal Action Plan with semi-structured interviews with local experts in agronomy, landscape architecture, geography, and regional planning, this study …

El Contexto Socioambiental De Los Resultados De Salud Materno-Infantil: Pasantía En El Hospital Simplemente Evita, González Catán, Athmika Krishnan

Argentina: Public Health in Urban Environments

La Matanza, especialmente el área de González Catán es una de las zonas más pobres en la provincia de Buenos Aires. Este portafolio investiga cómo el contexto socioambiental de un centro de cuidado público como el Hospital Simplemente Evita se manifiesta tanto a través del perfil de la paciente como las capacidades del hospital, en la recuperación de la enfermedad y la reforma social para resultados de salud materno-infantil.

From Deforestation To Regeneration: Modeling The Potential Of Chakra Agroforestry To Restore Edaphic Invertebrates And Soil Health In The Ecuadorian Amazon, Annika Vikstrom

Ecuador: Comparative Ecology and Conservation

Tropical edaphic invertebrates lie at the intersection of three historically understudied domains: tropical habitats, soil ecosystems, and terrestrial invertebrate biodiversity (Boyle et al. 2024; Lugo et al., 2024). Approximately 80% of tropical invertebrates remain undescribed, leaving major gaps in the understanding of how land-use change and climate change may affect their diversity and ecological function. The Amazon Basin, one of the world’s most biologically and culturally diverse regions, is increasingly threatened by land use change and deforestation. While agriculture is the second main driver of forest conversion in the basin, Indigenous Agroforestry systems such as Kichwa Chakras in the Ecuadorian …

Size Matters: Linkage Between Bromeliad Traits And The Spider Communities They Host In A Disturbed Habitat, Alesandra Fairchild

Ecuador: Comparative Ecology and Conservation

Bromeliads are a plant group that have been praised for their ability to create unique microhabitats, hosting an abundant and diverse faunal community due to their unique structural characteristics. Unfortunately, as deforestation rates rise, this plant group is at risk of decreasing in both abundance and diversity, which puts their associated organisms at risk of losing essential habitat (e.g. spiders). In this study, the effect of bromeliad structural characteristics and forest disturbance was assessed in a mature and secondary forest by classifying bromeliad abundance by forest type, characterizing forest structure, and by analyzing spider abundance and diversity based on these …

The Price Of Gold: Macroinvertebrate Responses To Mining In The Upper Jatunyacu River, Clara Chae

Ecuador: Comparative Ecology and Conservation

Gold mining is rapidly expanding in the upper Jatunyacu River basin of the Ecuadorian Amazon, an ecologically and culturally important region where Indigenous Kichwa communities depend on streams for drinking water, agriculture, and fisheries. This study assessed the ecological condition of five tributary streams across a variety of land uses: primary forest, chakra agroforestry, a stream recovering from historical mining, and a stream downstream of active small-scale gold mining. Benthic macroinvertebrates were collected and identified to morphospecies, and hydromorphological parameters, biodiversity indices, beta diversity metrics, and two biotic indices (AAMBI and BMWP/Col) were used to evaluate ecological integrity. Results showed …

What Do Rural Caregivers Need? Implications Of Formal Service Usage And The Role Of Cooperative Extension Systems, Naomi R. Meinertz, Jeong Eun Lee, Erin L. Martinez, Leacey Brown, Chelsey Byers, Sara Richie, Kathy Tutt

Journal of Human Sciences and Extension

The well-being of caregivers in the U.S. is a public health crisis. Caregivers experience greater rates of depression, chronic disease, and social isolation; however, the context of rural and urban living experiences creates different caregiving realities. Using two representative datasets of caregivers in the North Central and Northeast regions of the U.S., this paper examined the experiences of rural and urban caregivers in using formal support services. Rurality was negatively related to formal support service usage. Results from a multiple regression revealed a positive association between formal service utilization and caregiver well-being. These findings suggested that formal services can play …

A Comprehensive Nutrition Education Program Promotes Behavior Change And Perception Of Food Access Across The Older Adult Age Span, Sarah Misyak, Camille Mcguire, Victor Olayemi, Maria Denunzio, Paige Harrigan, Elena Serrano

Journal of Human Sciences and Extension

Strategies to promote healthy behaviors among older adults are needed to mitigate vulnerabilities to negative health outcomes, especially for those with low income. Healthy Eating and Staying Active as We Age (HESA), a nine-lesson educational series for Supplemental Nutrition Assistance Program-eligible older adults, was created to promote health among aging audiences. The purpose of this study was to evaluate the behavior change of older adults following participation in HESA, which covers food resource management, nutrition, food safety, and physical activity behaviors, as well as perceived food affordability and accessibility, and to determine if HESA is appropriate across the age span …

Creating Intergenerational Connections: A Multilevel Approach For Enriching Lives And Addressing Vital Community Issues, Matthew S. Kaplan, Nancy Z. Henkin

Journal of Human Sciences and Extension

This article presents an overview of intergenerational programs and practices as an interdisciplinary field of inquiry and practice that fosters open communication, support, and civic engagement across generations. Numerous examples of how intergenerational programs and practices harness age diversity to improve the human condition are presented. This includes initiatives to promote healthier lifestyles, reduce social isolation and loneliness, strengthen families, enrich education and lifelong learning, enhance community quality of life, and transmit cherished cultural traditions and values. This article highlights Extension’s longstanding contributions to intergenerational programming, showcasing notable examples across program areas and exploring opportunities for deeper engagement, co-leadership, and …

Intergenerational Creative Movement Program: A Social Connection Intervention To Foster Health And Well-Being, Claire M. Reardon, Mohammad Khalilian, Amy F. Kostelic, Susan Thiel, Anthony R. Bardo

Journal of Human Sciences and Extension

A sense of connection and belonging is widely recognized to be essential for mental health. Yet, since the COVID-19 pandemic, loneliness, social isolation, and mental health problems have remained elevated across America. Transitions into young adulthood and older age, while distinct, similarly challenge social connectedness, especially among women. Our multi-lesson education program connected first-generation female college students with college-educated forty-plus women to foster belonging and improve mental health. The program integrated dance as a creative physical activity, promoted intergenerational engagement through a humanistic focus on generativity, and used a social network approach to gauge the development of connections among participants …

Empowering Public Employees For Retirement Security: Evidence From A Pre-Retirement Financial Education Program, Suzanne Bartholomae, Mengya Wang

Journal of Human Sciences and Extension

The United States is facing a retirement crisis, with eight in ten older adult households experiencing or at risk of financial insecurity (Tavares et al., 2024). National data shows 60% of Americans regularly contribute to retirement accounts, and just 40% have estimated their retirement savings needs (Lin et al., 2022). With approximately 5,900 Baby Boomers retiring daily (Fry, 2019), there is considerable interest in identifying effective strategies and policies to address retirement preparedness. This paper presents evaluation findings from a retirement planning financial education program delivered to 750 public employees by Iowa’s public employee retirement system and Cooperative Extension educators. …

You’Re Only Old Once: And Other Truths About The Extension Work We Do In Aging, James S. Bates, Erin L. Martinez

Journal of Human Sciences and Extension

No abstract provided.

Mhc Gene Analysis And Immune Variation In Lepidodactylus Lugubris (The Mourning Gecko), Helen T. Vu

2025 Fall Honors Capstones Projects - Archive

The major histocompatibility complex (MHC) plays a central role in vertebrate immune defense by enabling pathogen recognition and initiating adaptive immune responses. While MHC diversity has been extensively studied in sexually reproducing species, comparatively little is known about its evolution in asexual lineages. The parthenogenetic gecko Lepidodactylus lugubris provides a unique opportunity to investigate how clonality and hybrid origins influence immune gene diversity. By leveraging whole-genome data, this study overcomes the limitations of previous transcriptome-based analyses and provides a more comprehensive view of MHC diversity in a clonal vertebrate. As the first step towards understanding MHC evolution in parthenogenetic lizards, …