Life Sciences Commons^™

Recombinant Pichia Pastoris Fermentation For Biosynthesis Of The Uv Resistant Compound Gadusol, Wenjie Zuo, Ping Zhang, Ruirui Xu, Leyi Zhang, Zhen Kang, Jianghua Li

Journal of Food Science and Biotechnology

[Objective ] This study aims to employ synthetic biology and metabolic engineering techniques to engineer Pichia pastoris (P.pastoris ) for the efficient heterologous synthesis of the UV resistant compound gadusol.[Method ] On the basis of a bioinformatic analysis and codon optimization of the genes involved in the gadusol biosynthetic pathway in Danio rerio,the gadusol fermentation pathway was constructed in P.pastoris via strategies such as promoter engineering,metabolic engineering,and fermentation optimization.[Result ] A high-purity gadusol standard was prepared through optimized extraction and chromatographic separation.The gadusol yield in the recombinant strain P.pastoris HME reached 105 mg/L.By screening of gene expression promoters and optimization …

Effects Of Critical Environmental Factors On The Antimicrobial Activity Of Ε-Poly- L-Lysine, Zhenjie Liu, Peihao Huang, Shu Chen, Shun Rong, Feiting Peng, Ling Chen, Weipeng Guo

Journal of Food Science and Biotechnology

[Objective ] This study explored the effects of critical environmental factors [water activity (aw),pH,and temperature ] on the antimicrobial activity of ε-poly- L-lysine (ε-PL),aiming to provide a basis for the application of ε-PL.[Method ] With Staphylococcus aureus as an indicator strain,the micro-broth dilution and agar dilution methods as well as antimicrobial growth kinetics were utilized to determine the antimicrobial activity of ε-PL.The protein leakage assay was employed to evaluate the bacterial membrane disruption activity of ε-PL.Live/Dead fluorescence staining was performed to observe the bacterial membrane permeability.[Result ] The minimum inhibitory mass concentration (MIC ) of ε-PL against Staphylococcus aureus was …

Probiotic Properties Of Three Strains Of Lactobacillus And Their Application In Corn Husk Fermentation, Bo Zhai, Quanxian Shu, Xiaoyan Yang, Yin Mao, Yu Deng, Yunying Zhao

Journal of Food Science and Biotechnology

[Objective ] This study aims to improve the utilization of maize resources and address the shortage of feed resources.[Method ] The intestinal colonization capacity,tolerance,and antibacterial properties of three Lactobacillus strains,Lactobacillus paracasei ZB- 1 (ZB-1),L.paracasei DY- 2 (DY-2),and L.rhamnosus DY- 4 (DY-4) were evaluated.The most suitable strain for fermenting cornhusks was identified,and its fermentation process was optimized.[Result ] ZB-1 exhibited higher intestinal colonization ability and antibacterial properties than DY- 2 and DY- 4.The antibacterial mechanism of ZB- 1 was likely driven by the action of organic acids in an acidic environment and certain protein-based antimicrobial substances.The fermentation conditions for ZB- 1 …

Diversity Of Fungi On The Surfaces Of Grapes And Raisins In Turpan , Xinjiang, Lei Sun, Laifang Qi, Qiuyan Liang, Jinxuan Han, Tianli Fan, Yuhua Ma

Journal of Food Science and Biotechnology

[Objective ] This study aims to understand the potential harmful fungi that may affect the quality of raisins during the drying process of grapes from Turpan,Xinjiang and to explore the relationships between varieties and the fungal community structure and diversity on the surfaces of grapes and raisins.[Method ] Four popular grape varieties (‘Thompson Seedles s’,‘Boert e’,‘Xiangfe i’,and ‘Seedless Blac k’) in a grapery in Turpan were selected as research objects.The fungal diversity on the surfaces of grapes and raisins was analyzed via high-throughput sequencing.[Result ] The dominant fungal phyla on the surfaces of grapes and raisins were Ascomycota and Basidiomycota.The …

Regulatory Effects Of Hesperetin And Eriocitrin On Gut Microbiota In An In Vitro Simulation Model, Zeren Cheng, Xia Meng, Kejing Tang, Ling Shen, Qing Gu, Ping Li

Journal of Food Science and Biotechnology

[Objective ] This study aims to compare the effects of hesperetin and eriocitrin on the gut microbiota in different enterotypes.[Method ] An in vitro gut fermentation model was established,which was combined with 16S rRNA high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS ) technique to evaluate the regulatory effects of the two flavonoids on the gut microbiota in different enterotypes.[Result ] After fermentation,both flavonoids significantly reduced the Firmicutes-to-Bacteroidota ratio (F/B ratio ) in terms of relative abundance at the phylum level in enterotype Bacteroides and enterotype Prevotella.At the genus level,pro-inflammatory bacteria including Paeniclostridium and Sutterella showed decreased relative abundance in treatment …

Effects Of Ultrasound-Assisted Enzymatic Extraction On The Physical And Chemical Properties Of Tortoise Shell Collagen, Xiong He, Rongxing Li, Shaotong Jiang, Jianfeng Lu, Lin Lin

Journal of Food Science and Biotechnology

[Objective ] The effects of different extraction methods on the yield,purity,structural characteristics,and physicochemical properties of tortoise shell collagen were investigated.[Method ] The ultrasound-assisted enzymatic method,acid method,and enzymatic method were used to extract collagen from tortoise shell powder.[Result ] The yield and purity of tortoise shell collagen extracted by ultrasound-assisted enzymatic method were 2.29% and 85.65%,respectively,significantly higher than those extracted by acid method (0.78% and 73.15%) and enzymatic method (1.77% and 75.63%).Ultraviolet absorption spectroscopy,infrared absorption spectroscopy,circular dichroism spectroscopy,and electrophoresis profiles showed that collagens extracted from tortoise shell by three methods were all type I collagen and retained intact triple helix structures.X-ray …

Building Swimming Skills In A Community Based Program For Black Youth: An Evaluation, Tyler Lennon Md, Mph, Ashley Hayes Mph, Leonardo Barrera Mph, Amy Hill Ms, Mph, Andy Miner, Michelle Macy Md, Ms

International Journal of Aquatic Research and Education

Black youth in the United States disproportionately experience fatal drowning at rates up to five times higher than their white peers. Eliminating these inequities requires new approaches that address historical and structural barriers of learning to swim. The objective of this study was to evaluate the three-week long swimming component of a community-based discovery program to empower Black youth in Evanston, IL. Participants were Black youth ages 8-10 years enrolled in summer 2023 and 2024. Swim skills were assessed by instructors using the Pictorial Scale of Perceived Water Competence (PSPWC). Caregiver surveys, collected post-intervention, also assessed perceived changes in swim …

An Innovative Approach Addressing Childhood Drowning: Making Use Of Collegiate Aquatic Facilities And Expertise, Gennette Cordova, Natasha Lloyd, Chezik Tsunoda

International Journal of Aquatic Research and Education

The silent epidemic of childhood drowning, and the racial disparities associated with it require scalable and innovative approaches that both break cycles of exclusion and circumvent our societal tendency to overlook drowning as a matter in need of urgent resolution. No More Under, a Seattle-based water safety organization whose mission is to eradicate childhood drowning, has launched the Swim Safely Series. The series is a national program uniquely poised to overcome the various barriers associated with racial and socioeconomic drowning disparities by tapping into underutilized collegiate aquatic spaces and merging water safety with mentorship and social intervention.

Understanding Callus Types In Maize By Genetic Mapping And Transcriptional Profiling, Guifang Lin, Yan Liu, Tej Man Tamang, Yang Qin, Mingxia Zhao, Hairong Wei, Et. Al.

Michigan Tech Publications

Plant transformation efficiency is highly dependent on species, individual genotypes, and tissue types. In maize, immature embryos are regularly used for transformation. The process relies heavily on callus development, as it is intricately associated with somatic embryogenesis and subsequent plant regeneration, both of which directly affect transformation efficiency. Immature embryos of the segregation progeny derived from the two inbred parents, a transformation-amenable line A188 and a recalcitrant line B73, can be cultured to form two primary callus types: Type I and Type II. The Type II callus grows faster and is a favorable type for regeneration. Here, Type I and …

Decadal Changes In Ground-Layer Plant Communities Reflect Maple Dieback And Earthworm Invasion In National Forests In The Lake Superior Region, Usa, Tara Bal, Manuel Eduardo Anderson, Mattison E. Brady, Julia I. Burton, Christopher R. Webster

Michigan Tech Publications

Northern hardwood forests of the Lake Superior region face a series of novel disturbance pressures including canopy dieback. Previous studies have linked regional sugar-maple (Acer saccharum) canopy dieback to introduced earthworms, which may have coinciding impacts on the ground-layer plant community. Dieback–earthworm interactions may lead to important longer-term changes in forest structure and function, but these relationships but have not been characterized. We sampled ground-layer plant communities in five national forest units in Michigan, Wisconsin, and Minnesota in 2010, and again just over a decade later in 2021. Non-metric multidimensional scaling ordination and indicator species analysis were used to assess …

Introducing Mcodegpt As A Zero-Shot Information Extraction From Clinical Free Text Data Tool For Cancer Research, Kai Zhang, Tongtong Huang, Bradley A Malin, Travis Osterman, Qi Long, Xiaoqian Jiang

Faculty, Staff and Student Publications

Background: The vast amount of natural language clinical notes about patients with cancer presents a challenge for efficient information extraction, standardization, and structuring. Traditional NLP methods require extensive annotation by domain experts for each type of named entity and necessitate model training, highlighting the need for an efficient and accurate extraction method.

Methods: This study introduces a tool based on the Large Language Model (LLM) for zero-shot information extraction from cancer-related clinical notes into structured data aligned with the minimal Common Oncology Data Elements (mCODE™) structure. We utilize the zero-shot learning capabilities of LLMs for information extraction, eliminating the need …

Stereoelectroencephalography Reveals Neural Signatures Of Multisensory Integration In The Human Superior Temporal Sulcus During Audiovisual Speech Perception, Yue Zhang, John F Magnotti, Xiang Zhang, Zhengjia Wang, Yingjia Yu, Kathryn A Davis, Sameer A Sheth, H Isaac Chen, Daniel Yoshor, Michael S Beauchamp

Faculty, Staff and Students Publications

Human speech perception is multisensory, integrating auditory information from the talker's voice with visual information from the talker's face. BOLD fMRI studies have implicated the superior temporal gyrus (STG) in processing auditory speech and the superior temporal sulcus (STS) in integrating auditory and visual speech, but as an indirect hemodynamic measure, fMRI is limited in its ability to track the rapid neural computations underlying speech perception. Using stereoelectroencephalography (sEEG) electrodes, we directly recorded from the STG and STS in 42 epilepsy patients (25F, 17M). Participants identified single words presented in auditory, visual, and audiovisual formats with and without added auditory …

Resilience And Vulnerabilities Of Tumor Cells Under Purine Shortage Stress, Jianpeng Yu, Chen Jin, Cheng Su, David Moon, Michael A Sun, Hong Zhang, Xue Jiang, Fan Zhang, Nomi Tserentsoodol, Michelle L Bowie, Christopher J Pirozzi, Daniel J George, Robert Wild, Xia Gao, David M Ashley, Yiping He, Jiaoti Huang

Faculty, Staff and Students Publications

Purpose: Purine metabolism is a promising therapeutic target in cancer; however, how cancer cells respond to purine shortage, particularly their adaptation and vulnerabilities, remains unclear.

Experimental design: Using the recently developed purine shortage-inducing prodrug DRP-104 and genetic approaches, we investigated the responses in prostate, lung, and glioma cancer models.

Results: We demonstrate that when de novo purine biosynthesis is compromised, cancer cells employ microtubules to assemble purinosomes, multiprotein complexes of de novo purine biosynthesis enzymes that enhance purine biosynthesis efficiency. Although this process enables tumor cells to adapt to purine shortage stress, it also renders them more susceptible to the …

Tng260 Is A Small-Molecule Corest Inhibitor That Sensitizes Stk11-Mutant Tumors To Anti-Pd-1 Immunotherapy, Leanne G Ahronian, Soumyadip Sahu, Minjie Zhang, Ayushi S Patel, Ke Geng, Reshmee Bhattacharya, Gerald S Falchook, Jonathan W Goldman, Alexander I Spira, Salman R Punekar, David R Spigel, Judy S Wang, Ferdinandos Skoulidis, Janaye Stephens, Mary Meynardie, Jaylen M Powell, Alfonso Lopez, Michela Ranieri, Magdalena A Ploszaj, Yi Jer Tan, Yeuan Ting Lee, Yi Yu, Jiehui Deng, Ting Chen, Patrick Mccarren, Alice Tsai, Suleman S Hussain, Brian Doyon, Kenjie Amemiya, Jacques Ermolieff, Preksha Shahagadkar, Nikitha M Das, Lauren R Flynn, Julie A Shields, Laney Danielczyk, Brian J Mcmillan, Andre Mignault, Samuel R Meier, Hsin-Jung Wu, David J Guerin, Douglas A Whittington, Chengyin Min, Iga Sienczylo, John P Maxwell, Heather J Dibenedetto, Hideo Watanabe, Brian B Haines, Alan Huang, Adam Crystal, Jannik N Andersen, Xinyuan Wu, Kwok-Kin Wong

Faculty, Staff and Student Publications

Patients with non–small cell lung cancer (NSCLC) with loss of the tumor suppressor gene STK11 are resistant to immune checkpoint therapies like anti–PD-1. In this study, we conducted an in vivo CRISPR screen that identified histone deacetylase 1 as a target to reverse anti–PD-1 resistance driven by loss of STK11 and developed TNG260, a potent small-molecule inhibitor of the CoREST complex with selectivity exceeding previously generated inhibitors in this class in preclinical studies. Treatment with TNG260 led to increased expression of immunomodulatory genes in STK11-deficient cancer cells. When combined with anti–PD-1, TNG260 induced immune-mediated stasis and/or regression in STK11 …

Preoperative Brain Mapping Predicts Language Outcomes After Eloquent Tumor Resection, Matthew T Muir, Kyle Noll, Sarah Prinsloo, Hayley Michener, Jeffrey I Traylor, Vinodh A Kumar, Chibawanye I Ene, Sherise Ferguson, Ho-Ling Liu, Jeffrey S Weinberg, Frederick Lang, Brian A Taylor, Stephanie J Forkel, Sujit S Prabhu

Faculty, Staff and Student Publications

When operating on gliomas near critical language regions, surgeons risk either leaving residual tumor or inducing permanent postoperative language deficits (PLDs). Despite the advent of intraoperative mapping techniques, subjective judgments frequently determine important surgical decisions. We aim to inform data-driven surgery by constructing a non-invasive mapping approach that quantitatively predicts the impact of individual surgical decisions on long-term language function. This study included 79 consecutive patients undergoing resection of language-eloquent gliomas. Patients underwent preoperative navigated transcranial magnetic stimulation (TMS) language mapping to identify language-positive sites ("TMS points") and their associated white matter tracts ("TMS tracts") as well as formal language …

The Isolation And Dna Extraction Of Phytophthora From Four Wildland Soil Types In Northern California, Humboldt County, Angelina Lasko, Christopher Lee, David S. Baston, Susan E. Marshall

Creative Publications and Manuals

Phytophthora species are of global importance (Sinclair and Lyon, 2005). For most Phytophthora species, the natural mechanism of how these pathogens travel and spread long distances is not fully understood. They are have been shown to travel by air, water, nursery stock, and via inadvertent human transport. The purpose of this study was to identify the presence of Phytophthora species in four different Northern California forest/wildland soil types. Soils from Franciscan complex parent material (riparian, mixed conifer, and oak woodland), and serpentine parent material (mixed conifer) were sampled in Humboldt County (Fig 1). Phytophthora and other pathogens were baited from …

Cv: Marne Bailey (Biology), Marne Bailey

Biology Department Faculty Curricula Vitae

No abstract provided.

Scalable Biosynthesis And Recovery Of Poly-3-Hydroxybutyrate Produced From Cotton-Derived Glucose By Cupriavidus Necator, Ashley M. Clark, Lucia E. Gargano, Gabriella M. Fioravanti, Hannah M. Schapiro, Ron G. Kander

School of Design and Engineering Papers

To combat the growing issue of petroleum plastic waste, alternative bio-based polymers are being developed. Many of these biopolymers are made from bio-derived materials, or are biodegradable, but the most promising polymers fall in both categories. Polyhydroxyalkanoates (PHAs) are one such class of polymers, and poly-3-hydroxybutyrate (P3HB), the most popular PHA, has shown great potential. This study utilized two types of cotton-derived glucose, alongside commercial glucose, as a feedstock for the biosynthesis of P3HB by Cupriavidus necator (also known as Ralstonia eutropha). The fermentation took place in a 2-L bioreactor, showing potential for scale-up. A single-solvent extraction method was …

An Examination Of The Experiences Of An African American Male Diver On A Power Four Swim Team: An Elite Interview, Steven N. Waller Ph.D., Tiffany M. Quash Ph.D.

International Journal of Aquatic Research and Education

Despite the incremental increase in the number of Black people who are learning to swim, the number of Black divers has not kept pace. In the NCAA Database, swimmers and divers were not segmented; thus, they all appear in the category of “swimming” by race and sex. When viewing the 2024 Division I NCAA Demographics Database, 89 (.02%) of 4307 student-athletes participating in swimming were Black women. For men, the data is equally dismal, revealing that 81 (.03%) of 2,608 student-athletes who were identified as swimmers were Black. The purpose of this research note is to examine the developmental experiences …

Quantifying Interest In And Sentiment Of Online Media About Greenhouse Gas Emissions From Cattle Production In The United States, Michael L. Smith, Jinho Jung, Nicole Olynk Widmar, Danielle J. Ufer, Maria Berikou, Jayson Lusk

Department of Agricultural Economics Faculty Publications

The public is paying increased attention to the environmental impacts associated with cattle production systems in the US. Thus, cattle producers are increasingly exploring ways to communicate their efforts to reduce those environmental and climate impacts. This study uses online and social media data from 2018 to 2022 to examine public interest in and sentiment about beef and dairy cattle production when discussed within the context of greenhouse gas (GHG) emissions. Variation in mentions and net sentiment over the reporting period is quantified and analyzed. Over the period of data collection, which was interrupted by the COVID-19 pandemic, we find …

Molecular Determinants Of Neoadjuvant Chemotherapy Resistance In Breast Cancer: An Analysis Of Gene Expression And Tumor Microenvironment, Hedda Michelle Guevara-Nieto, Carlos A. Orozco-Castaño, Rafael Parra-Medina, Jenny Nathaly Poveda-Garavito, Jone Garai, Jovanny Zabaleta, Liliana López-Kleine, Alba Lucia Combita

School of Graduate Studies Faculty Publications

Neoadjuvant chemotherapy (NAC) is a critical component of breast cancer treatment, but the molecular mechanisms underlying resistance remain poorly understood. This study aimed to identify transcriptomic changes associated with NAC resistance across four breast cancer subtypes: Luminal A, Luminal B/HER2-positive, Luminal B/HER2-negative, and Triple-Negative Breast Cancer (TNBC). RNA-seq analysis was performed on paired pre- and post-NAC breast cancer samples from 32 nonresponders. Differentially expressed genes (DEGs) were identified, and functional enrichment analyses were conducted. Protein-protein interaction (PPI) networks were constructed to identify hub genes. Tumor microenvironment (TME) infiltration was estimated using deconvolution algorithms. The results revealed distinct gene expression profiles …

Evaluation Of Anti-Bacterial Efficacy Of Ginger, Resin Gum, And Nigella Sativa Irrigants Against Enterococcus Faecalis: An In Vitro Study, Iman El Ghazzawy, Leen Imad Hasbini, Reem Chamseddine, Wael Betbout, Roula Abiad

International Arab Journal of Dentistry

Objectives: To evaluate the antibacterial efficacy of Ginger, Resin Gum, and Nigella Sativa, against Enterococcus Faecalis.

Methods: Thirty-eight extracted single-rooted premolars were inoculated with E.faecalis, except the negative control, and incubated for 4 weeks. After instrumentation samples were divided into 4 groups (n=7) according to final irrigation: NaOCl+EDTA (Group A), Nigella sativa (B), Resin Gum (C), Ginger (D), plus 2 control groups (n=5): Saline (E), and Saline without bacterial inoculation (F). Cultures were collected at baseline and after irrigation.

Results: One-way ANOVA showed that in groups A-D a statistically significant reduction of bacterial load was observed at …

Landscape And Clinicopathologic Features Of Ras Pathway Mutations In Chronic Myelomonocytic Leukemia, Guillermo Montalban-Bravo, Sanam Loghavi, Ziyi Li, Kelly Chien, Rashmi Kanagal-Shamanna, Alex Bataller, Anuya Natu, Mark Gurney, Alexandre Bazinet, Danielle Hammond, Koji Sasaki, Gautam Borthakur, Mahesh Swaminathan, Courtney Dinardo, Tapan Kadia, Farhad Ravandi, Naval Daver, Nicholas Short, Naveen Pemmaraju, Ghayas Issa, Terra L Lasho, Christy M Finke, Aref Al-Kali, Clifford Csizmar, Hassan Alkhateeb, Naseema Gangat, Abhishek A Mangaonkar, Carlos Bueso-Ramos, Ayalew Tefferi, Hagop Kantarjian, Guillermo Garcia-Manero, Mrinal M Patnaik

Faculty, Staff and Student Publications

RAS pathway (RASp) mutations induce proliferative features, and promote transformation in chronic myelomonocytic leukemia (CMML). However, the unique clonal landscape and hierarchy of distinct RASp mutations remain unexplored. To characterize the landscape, architecture, and implications of unique RASp mutations in CMML, we evaluated a cohort of 814 patients with CMML. We identified 461 RASp mutations among 342 patients (42%). N/KRAS and CBL mutations were the most common, frequently involved the P-loop or RING domains, respectively, and frequently appeared as dominant events (63% and 65%, respectively). BRAF, NF1, and PTPN11 mutations spanned throughout the gene structure, and frequently appeared as subclonal …

Yx0798 Is A Highly Potent, Selective, And Orally Effective Cdk9 Inhibitor For Treating Aggressive Lymphoma, Vivian Jiang, Yu Xue, Hong Kim, Qingsong Cai, Tianci Zhang, Lei Nie, Joseph Mcintosh, Yang Liu, Haiying Chen, Jia Zhou, Michael Wang

Faculty, Staff and Student Publications

Nongenetic transcription evolution has been increasingly explored and recognized to drive tumor cell progression and therapeutic resistance. As the regulation hub of transcription machinery, cyclin-dependent kinase 9 (CDK9) is the gatekeeper of RNA polymerase II transcription, and CDK9 dysfunction results in transcriptomic reprogramming and tumor cell progression. We recently reported that the heat shock protein 90 (HSP90)-MYC-CDK9 network drives therapeutic resistance in mantle cell lymphoma (MCL) through transcriptomic reprogramming. We also showed that targeting CDK9 by AZD4573 and enitociclib is a safe and effective treatment in preclinical Mantle Cell Lymphoma (MCL) models, supporting CDK9 as a valid therapeutic target for …

Optimizing Genetic Ancestry Adjustment In Dna Methylation Studies: A Comparative Analysis Of Approaches, Kira D Höffler, Seyma Katrinli, Matthew W Halvorsen, Anne-Kristin Stavrum, Kevin S O'Connell, Alexey Shadrin, Srdjan Djurovic, Ole A Andreassen, James J Crowley, Jan Haavik, Kristen Hagen, Gerd Kvale, Kerry Ressler, Bjarne Hansen, Jair C Soares, Gabriel R Fries, Alicia K Smith, Stéphanie Le Hellard

Faculty, Staff and Student Publications

Background: Genetic ancestry is an important factor to account for in DNA methylation studies because genetic variation influences DNA methylation patterns. One approach uses principal components (PCs) calculated from CpG sites that overlap with common SNPs to adjust for ancestry when genotyping data is not available. However, this method does not remove technical and biological variations, such as sex and age, prior to calculating the PCs. The first PC is therefore often associated with factors other than ancestry.

Methods: We developed and adapted the adapted EpiAnceR+ approach, which includes (1) residualizing the CpG data overlapping with common SNPs for control …

Needs Analysis Of Interactive Learning Media For Teachingheat Transfer In Physics: Students’ And Teachers’Perspectives, Rizki Eka Shintya, Ika Mustika Sari, Ida Kaniawati

Jurnal Pendidikan Sains

Heat transfer material in physics is known to be abstract. This study specifically analyzes the conceptual difficulties, and visualization needs specific to this material, which is different from the analysis of physics needs in general. This analysis does not only look for the need for traditional media but explicitly looks for the need for interactive media (simulation, animation, virtual laboratory). Therefore, this study aims to identify the need for interactive learning media in physics education, specifically in the teaching of heat transfer, from the perspective of students and teachers in West Java, Indonesia. Using a quantitative descriptive survey, data were …

Efficacy Of Teaching At The Right Level Approach Combined With The Problem-Based Learning Model On Science Learning Outcomes At Middle School, Heri Syahputra Azhar Panjaitan, Ryan Dwi Puspita

Jurnal Pendidikan Sains

This research seeks to evaluate the efficacy of the Teaching at the Right Level (TaRL) strategy in conjunction with the Problem-Based Learning (PBL) model on the scientific learning outcomes of eighth-grade students at middle school Islam Terpadu Khairul Imam. This research addresses the inadequate scientific problem-solving abilities and science learning outcomes of Indonesian students, necessitating a more interactive and individualized instructional technique. The methodology used is a quasi-experimental design with a nonequivalent control group. The sample included two purposively chosen eighth-grade classes: one experimental class (n=25) underwent the TaRL–PBL intervention, whereas the control class (n=25) simply used the lecture approach. …

Synergistic Effects Of Orifice Geometry And Inclination Angle On The Damping Performance Of Tuned Liquid Column Vibration Absorbers, Mohamed M. Abou Elenin, Younes K. Younes, Mohamed F. Younes

Mansoura Engineering Journal

This study experimentally investigates the dynamic damping performance of passive tuned liquid column vibration absorbers (TLCVAs), focusing on the influence of vertical column inclination angle and orifice disc configuration. Free vibration tests were conducted on a primary system equipped with a TLCVA using a custom-designed test rig. The experimental framework systematically varied inclination angles from to and tested both single-hole and multi-perforated orifice discs, each designed with an equivalent total flow area. A MATLAB-based signal processing framework was employed to analyze time-domain responses, and the logarithmic decrement method was applied to determine the damping ratio of the primary system. The …

Tableau Part Ii - October 2025, Rubab Shahzad

Day Family Research Lab Workshop Series

Part Two of Introduction to Tableau. Learn to make cool visualizations using Tableau. A hands-on opportunity where we will go over calculated fields, hierarchies, unions, dashboards, and stories.

Prior experience with Tableau is recommended

Climate Change Projections Through Optimized Machine Learning And Deep Learning Models: A Comprehensive Analysis, Hala H. Youssef, Salma S. Mohammed, Hanan M. Amer, Abeer T. Khalil

Mansoura Engineering Journal

One major worldwide issue that has far-reaching effects on environmental stability and the avoidance of natural disasters is climate change. Developing efficient mitigating strategies depends on precise climate change forecasts. To predict important climate change indicators, including temperature fluctuations, greenhouse gas (CO2, N2O, CH4, and SF6) emissions, population dynamics, sea level changes, Arctic Sea ice extent, and Antarctica mass, this study evaluated the predictive capabilities of several Machine Learning (ML) and Deep Learning (DL) techniques. The set of machine learning algorithms includes Multiple Linear Regression (MLR), K-Nearest Neighbors (KNN), Support Vector Machines (SVMs), Artificial Neural Networks (ANN), Random Forest (RF), …