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Full-Text Articles in Life Sciences

Comparison Of Summer Forages And The Effect Of Nitrogen Fertilizers On Brassica Forages In Tasmania, A. A. Salardini, R. J. Eckard, D. R. Franks Oct 2021

Comparison Of Summer Forages And The Effect Of Nitrogen Fertilizers On Brassica Forages In Tasmania, A. A. Salardini, R. J. Eckard, D. R. Franks

IGC Proceedings (1977-2023)

Summer forage crops, and in particular Brassica spp., have become increasingly popular in dairy production systems in Tasmania. Field experiments were conducted for 3 years in northwestern Tasmania, in the spring/summers beginning in 1995. The study aimed to compare yield and quality of Brassica and Poaceae forages and the response of Brassica species to nitrogen (N) (50, 100 and 200 kg N/ha) and irrigation.

The average total yields of dryland (rainfed) crops in 1995 to 1997 experiments, were turnip (Brassica rapa) 9.3 t/ha, rape (B. napus) 5.9 t/ha, oats (Aevena sativa) 5.2 t/ha, kale …


Maturity And Fertilization Effects On Leaf Blade Tissue Percentages Of Contrasting Fescue Cultivars, L. B. Tempone, M. S. Cid, M. A. Brizuela, M. C. Nunciari, M. G. Cendoya Oct 2021

Maturity And Fertilization Effects On Leaf Blade Tissue Percentages Of Contrasting Fescue Cultivars, L. B. Tempone, M. S. Cid, M. A. Brizuela, M. C. Nunciari, M. G. Cendoya

IGC Proceedings (1977-2023)

Palenque Plus and Ceres Torpedo are tall fescue (Festuca arundinacea Schreb.) cultivars available to the animal production systems in the humid Pampean Region of Argentina. They differ in growth cycle and morphological characteristics. At vegetative stage, Torpedo leaves are fine and tender, which suggests that their percentages of slowly digestible and indigestible tissues (SDIT) should be low. In this study we 1) explored the effects of phenological stage and nitrogen fertilization on leaf blade tissue percentages of both cultivars, using an ordination multivariate technique, and 2) evaluated the effects of these factors on the percentage of SDIT. Our results …


Different Harvest Schedules To Prepare Deferred Forage From C4 Grasses In Córdoba, Argentina, M. R. Steinberg, E. F. Nienstedt, H. A. Valdez, J. C. Coraglio, C. A. Vieyra, P. A. Minuzzi Oct 2021

Different Harvest Schedules To Prepare Deferred Forage From C4 Grasses In Córdoba, Argentina, M. R. Steinberg, E. F. Nienstedt, H. A. Valdez, J. C. Coraglio, C. A. Vieyra, P. A. Minuzzi

IGC Proceedings (1977-2023)

From 1995 until 1999 a trial was done in the fields of the Universidad Nacional de Córdoba with four C4 forage grasses. These fields lie in the subtropical and semiarid region of Argentina. This research aimed to identify forage species that could be used as deferred forage for the drier and cooler winter season. Four species of C4 grasses were used: Rhodesgrass (Chloris gayana Kunth), Gatton (Panicum maximum), coloratum (Panicum coloratum) and digitgrass (Digitaria eriantha), respectively. Two growing periods were used: full season growth (FS) and half season regrowth (HS), in both cases …


Dietary Diversity And Nutrient Intake Of Han And Dongxiang Smallholder Farmers In Poverty Areas Of Northwest China, Zhuo Wang, Youhai Chen, Shihua Tang, Siqi Chen, Shaoqing Gong, Xinyin Jiang, Liang Wang, Ying Zhang Oct 2021

Dietary Diversity And Nutrient Intake Of Han And Dongxiang Smallholder Farmers In Poverty Areas Of Northwest China, Zhuo Wang, Youhai Chen, Shihua Tang, Siqi Chen, Shaoqing Gong, Xinyin Jiang, Liang Wang, Ying Zhang

Publications and Research

This study aimed to evaluate the status of dietary diversity and nutrient intake among Han and Dongxiang smallholder farmers in poor rural areas of northwest China. In this cross-sectional study, dietary intake was assessed in 499 smallholder farmers aged 18–75 years from two nationally designated impoverished counties in Gansu Province, China, using three consecutive 24 h dietary recalls. The dietary diversity score (DDS) and nutrient adequacy ratio (NAR) were adopted to assess dietary diversity and micronutrient adequacy, respectively. The mean DDS (range from 1 to 9) in participants was relatively low (3.81 ± 1.01). Consumption of grains was excessive, while …


Factors Determining Successful Exclusive Breastfeeding Among Working Mothers In Indonesia: An Exploration By Using Positive Deviance Concept: Analysis Of Secondary Data Of Demographic Health Survey Indonesia 2017, Melinda Mastan, Endang Laksminingsih Achadi Oct 2021

Factors Determining Successful Exclusive Breastfeeding Among Working Mothers In Indonesia: An Exploration By Using Positive Deviance Concept: Analysis Of Secondary Data Of Demographic Health Survey Indonesia 2017, Melinda Mastan, Endang Laksminingsih Achadi

Indonesian Journal of Public Health Nutrition (IJPHN)

Despite the fact that exclusive breastfeeding brings a lot of benefits for infants and mothers, breastfeeding rates in Indonesia remain low, at 42% of all infants aged 0-5 months in 2012. This study aims to apply the positive deviance concept to explore the factors determining the success of exclusive breastfeeding among mothers in a high-risk group in Indonesia. The study used the cross-sectional, DHS 2017 data of 1.178 mothers with 0-5 months old infants in Indonesia. Chi-square and multiple logistic regression tests with 95% confidence intervals are used in identifying the factors associated with exclusive breastfeeding. Maternal working status is …


The Effect Of The Provision Of Bagea Enriched With Sea Urchin Gonads On Weight Gain In Toddlers Of The Bajo Ethnic, La Banudi, Suriana Koro, M. Anas Anasiru, Nurmiaty Nurmiaty Oct 2021

The Effect Of The Provision Of Bagea Enriched With Sea Urchin Gonads On Weight Gain In Toddlers Of The Bajo Ethnic, La Banudi, Suriana Koro, M. Anas Anasiru, Nurmiaty Nurmiaty

Indonesian Journal of Public Health Nutrition (IJPHN)

Cassava starch extract and sea urchin gonads can be used as ingredients for making various kinds of food formulas. This study aimed to develop cassava starch extract as a formula for making bagea added with sea urchin gonads which are rich in nutrients to make it good for child. This study was aimed at analyzing the effect of the provision of bagea enriched by sea urchin gonads on weight gain in toddlers of the Bajo community. In making the formula of bagea, the researchers used a quasi-experimental design. After the data were collected, they were then checked for feasibility, coded, …


Relationship Of Age, Gender, And History Of Comorbid Diseases In Tb Patients Toward Self-Stigma Tb In Surakarta, Ignes Widowati, Balgis Balgis, Sri Mulyani Oct 2021

Relationship Of Age, Gender, And History Of Comorbid Diseases In Tb Patients Toward Self-Stigma Tb In Surakarta, Ignes Widowati, Balgis Balgis, Sri Mulyani

Indonesian Journal of Public Health Nutrition (IJPHN)

Tuberculosis (TB) infection in Indonesia has not been completely eradicated. It is challenging for those who suffer from TB to be away from self-stigma. This study aimed to determine the relationship between age factors, gender, and a history of comorbid diseases related to self-stigma. This was a cross sectional study using a Tuberculosis Stigma Assessment questionnaire. Total 50 respondents were obtained by purposive sampling technique. Inclusion criteria are TB patients or former patients aged 18 years to the elderly, male and female, without or having a history of comorbidities (HIV & DM). Quantitative-qualitative analysis, univariate and bivariate tests using Pearson …


Law Enforcement In Relation To Illegal Unreported And Unregulated (Iuu) Fishing In Sierra Leone, Mahmoud Papa Conteh Oct 2021

Law Enforcement In Relation To Illegal Unreported And Unregulated (Iuu) Fishing In Sierra Leone, Mahmoud Papa Conteh

World Maritime University Dissertations

No abstract provided.


Optimising Risk Governance : A Case Study Of The Philippines’ Change Crew Hub System, Cynthia E. Esquivias Oct 2021

Optimising Risk Governance : A Case Study Of The Philippines’ Change Crew Hub System, Cynthia E. Esquivias

World Maritime University Dissertations

No abstract provided.


An Effective Method To Predict Low Birth Weight In Indonesia Rural Area, Asyifa Robiatul Adawiyah, Kusharisupeni Djokosujono, Nur Alam, Novita Arie Setiawati Oct 2021

An Effective Method To Predict Low Birth Weight In Indonesia Rural Area, Asyifa Robiatul Adawiyah, Kusharisupeni Djokosujono, Nur Alam, Novita Arie Setiawati

Indonesian Journal of Public Health Nutrition (IJPHN)

Antenatal care (ANC) is a compulsory program in Indonesia with minimally 4 times visits, unfortunately, regular Antenatal care visit at Kanjillo health center, South Sulawesi, Indonesia is still low. Most of the Low Birth Weight (LBW) cases in Indonesia happened in a rural-areas, where most of the women conducted the first visit ANC on the third trimester. This study aimed to determine the cut-off point of mother’s last trimester pregnancy weight for predicting as well as preventing low birthweight outcomes in the community. The research design was cross-sectional with secondary data taken from the Kanjilo health center, with 150 pregnant …


Faktor-Faktor Yang Berhubungan Dengan Kejadian Diare Pada Siswa Sekolah Dasar Di Kabupaten Lebak, Provinsi Banten, Indonesia, Ilham Ibrahim, Ratu Ayu Dewi Sartika, Triyanti Triyanti, Tria Astika Endah Permatasari Oct 2021

Faktor-Faktor Yang Berhubungan Dengan Kejadian Diare Pada Siswa Sekolah Dasar Di Kabupaten Lebak, Provinsi Banten, Indonesia, Ilham Ibrahim, Ratu Ayu Dewi Sartika, Triyanti Triyanti, Tria Astika Endah Permatasari

Indonesian Journal of Public Health Nutrition (IJPHN)

Diarrhea is an endemic disease that has the potential to create extraordinary events in a country, including Indonesia. The highest diarrhea case was found in Banten Province, namely in Lebak Regency, the highest number was in the school-age group. This study aims to determine the relationship between the incidence of diarrhea on children's characteristics, children's behavior characteristics, mother/family characteristics, and environmental characteristics. This study uses secondary data with a cross-sectional design with a total sample of 151 elementary school children. The research was conducted in Cidahu Village RT/RW 02/01 Karangkamulyan Village, Lebak Regency, Banten Province. The dependent variable is the …


Current Situation And Solutions In Vietnam's National Legislation On Fisheries Subsidies To Prevent Illegal, Unreported And Unregulated (Iuu) Fishing, Tien Hoang Nguyen Oct 2021

Current Situation And Solutions In Vietnam's National Legislation On Fisheries Subsidies To Prevent Illegal, Unreported And Unregulated (Iuu) Fishing, Tien Hoang Nguyen

World Maritime University Dissertations

No abstract provided.


Smokejumper Obituary: Bell, Douglas E. “Doug” (Missoula 0000), National Smokejumper Association Oct 2021

Smokejumper Obituary: Bell, Douglas E. “Doug” (Missoula 0000), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.


Fisherfolk Awareness And Participation : Identifying The Barriers Of Unreported And Underreported Fishing In Guyana Small-Scale Artisanal Fishery, Randy Terry Bumbury Oct 2021

Fisherfolk Awareness And Participation : Identifying The Barriers Of Unreported And Underreported Fishing In Guyana Small-Scale Artisanal Fishery, Randy Terry Bumbury

World Maritime University Dissertations

No abstract provided.


Regulating Iuu Fishing In Nigeria : A Step Towards Discovering The Untapped Potentials Of Fisheries In Nigeria, Gabriel Chukwuemeka Chikelu Oct 2021

Regulating Iuu Fishing In Nigeria : A Step Towards Discovering The Untapped Potentials Of Fisheries In Nigeria, Gabriel Chukwuemeka Chikelu

World Maritime University Dissertations

No abstract provided.


Cage Fish Farming In Lake Victoria, Tanzania : A Recently Rising Food Production Area And Its Effects On The Environment And Community., Kwilasa Lushanga Ng'wigulu Oct 2021

Cage Fish Farming In Lake Victoria, Tanzania : A Recently Rising Food Production Area And Its Effects On The Environment And Community., Kwilasa Lushanga Ng'wigulu

World Maritime University Dissertations

No abstract provided.


Understanding Human Impacts On The Marine Environment In Fiji : Insights From An Odemm Conceptual Model And Semi-Structured Interviews, Alumita Talei Sekinairai Oct 2021

Understanding Human Impacts On The Marine Environment In Fiji : Insights From An Odemm Conceptual Model And Semi-Structured Interviews, Alumita Talei Sekinairai

World Maritime University Dissertations

No abstract provided.


An Assessment Of The Marine Protected Area Ecological Monitoring Framework In Kenya, Tracy Masicha Wafula Oct 2021

An Assessment Of The Marine Protected Area Ecological Monitoring Framework In Kenya, Tracy Masicha Wafula

World Maritime University Dissertations

No abstract provided.


10th International Conference On Business, Technology And Innovation 2021, University For Business And Technology - Ubt Oct 2021

10th International Conference On Business, Technology And Innovation 2021, University For Business And Technology - Ubt

UBT International Conference

Welcome to IC – UBT 2021

UBT Annual International Conference is the 10th international interdisciplinary peer reviewed conference which publishes works of the scientists as well as practitioners in the area where UBT is active in Education, Research and Development. The UBT aims to implement an integrated strategy to establish itself as an internationally competitive, research-intensive university, committed to the transfer of knowledge and the provision of a world-class education to the most talented students from all background. The main perspective of the conference is to connect the scientists and practitioners from different disciplines in the same place and make …


Boron Fertilization Effects On Nutritive Parameters Of Alfalfa, F. M. Rouquette Jr., V. A. Haby, J. O. Fritz, M. Collins Oct 2021

Boron Fertilization Effects On Nutritive Parameters Of Alfalfa, F. M. Rouquette Jr., V. A. Haby, J. O. Fritz, M. Collins

IGC Proceedings (1977-2023)

With early pre-plant incorporation of limestone to raise soil pH to 6.8 to 7.0, alfalfa (Medicago sativa L.) may be grown successfully on acid, Coastal Plain, sandy loam soils. Fertility and forage quality for hay and/or grazing require alternative management of alfalfa on these Coastal Plain soils. A two-year experiment evaluated alfalfa fertilized with three rates of boron to quantify effects on nutritive entities, especially fiber components. On a whole-plant basis in year 1, only crude protein was increased (P < .05) at one of six harvest dates. In year 2, chemical analyses of leaf-stem components indicated consistent increases in crude protein of leaf fractions at each harvest (P < .05), reduction in neutral detergent fiber at three harvest dates (P < .06), and reduced lignin (P < .07) at the final harvest. Stem sections were affected by rate of boron at only the June harvest with an increase in crude protein (P < .05). Rate of boron fertilization had indirect effects on nutritive value (crude protein) by either delaying physiological maturity or enhancing nitrogen fixation in leaves, and had a direct negative effect on certain fiber components.


The Role Of Grass Feeding In Improving Oxidative Stability And Increasing Vitamin B12 Content Of Beef And Veal, V. Pastushenko, H.-D. Matthes, H. Heinrich, Z. Holzer, T. Prokopyuk Oct 2021

The Role Of Grass Feeding In Improving Oxidative Stability And Increasing Vitamin B12 Content Of Beef And Veal, V. Pastushenko, H.-D. Matthes, H. Heinrich, Z. Holzer, T. Prokopyuk

IGC Proceedings (1977-2023)

The objective of this study was to investigate the influence of grass feeding of cattle on the decontamination capacity against free radical (DCAFR) and vitamin B12 content of beef and veal. The meat samples from intensively (26 cattle, final weight 485±9 kg, concentrate feeding) and ecologically (25 heifers, final weight 472±32 kg, and 28 calves, final weight 260±24 kg, pasture grazing) managed cattle groups were investigated. The DCAFR was colorimetric assessed by examining damage to 1.4 benzoquinone. Vitamin B12 was analyzed by high performance liquid chromatography. Data indicate that the oxidative stability of meat samples from ecological management …


Nitrate Accumulation In A Sorghum-Sudangrass Hybrid As Influenced By Nitrogen Fertilization, A. Elgersma, I. Y. Tecle, T. Provin Oct 2021

Nitrate Accumulation In A Sorghum-Sudangrass Hybrid As Influenced By Nitrogen Fertilization, A. Elgersma, I. Y. Tecle, T. Provin

IGC Proceedings (1977-2023)

The effect of nitrogen (N) fertilization and plant stage on nitrate accumulation was studied in a sorghum-Sudan grass hybrid. Grass cv. Dynagrazer was sown at the experimental site of Texas A & M University. Six N levels were applied (0, 112 … 672 Kg. N ha-1). Nitrate concentrations were determined in three harvests, 5– 7 weeks after planting. Maximum levels were 2.1, 3.5 and 4.6 g nitrate-N kg-1 DM, respectively, which is beyond the safe limits for animal consumption. The plots were cut at heading. Regrowth took place on residual N. Nitrate-N accumulation was lowest in the …


Effect Of Maceration On In Sacco Degradability And Energy Content Of Low-Moisture Round Bale Alfalfa Silage, K. Nonaka, S. Kume, T. Ohshita Oct 2021

Effect Of Maceration On In Sacco Degradability And Energy Content Of Low-Moisture Round Bale Alfalfa Silage, K. Nonaka, S. Kume, T. Ohshita

IGC Proceedings (1977-2023)

The aim of this study was to determine the effect of maceration on in sacco degradability and energy content of low-moisture alfalfa (Medicago sativa L.) silage. The results of two treatments were compared: 1) maceration, in which the alfalfa was processed through a large- scale forage mat maker prior to wilting to approximately 50% DM content (FM treatment), and 2) conventional conditioning, in which the alfalfa was tedded and wilted to the same DM content as that in the FM treatment (CC treatment). Silage bales were chopped in 20-mm pieces for each experiment. In sacco degradabilities of DM, CP …


Efficiency Of Nitrogen Use In Dairy Cows Grazing Ryegrass With Different Water Soluble Carbohydrate Concentrations, L. A. Miller, D. H. Baker, M. K. Theodorou, J. C. Macrae, M. O. Humphreys, Nigel D. Scollan, J. M. Moorby Oct 2021

Efficiency Of Nitrogen Use In Dairy Cows Grazing Ryegrass With Different Water Soluble Carbohydrate Concentrations, L. A. Miller, D. H. Baker, M. K. Theodorou, J. C. Macrae, M. O. Humphreys, Nigel D. Scollan, J. M. Moorby

IGC Proceedings (1977-2023)

This experiment is one of a series designed to investigate the efficiency of nitrogen (N) use in Holstein-Friesian dairy cows grazing perennial ryegrass (Lolium perenne) which has been bred to express high water soluble carbohydrate (WSC) concentrations. Animals grazed either a High Sugar (HS) grass or a Control (C) variety chosen on the basis of heading date compatibility. Dry matter (DM) intakes were estimated using herbage mass. Milk yields, milk constituent concentrations and plasma concentrations of b-hydroxybutyrate, glucose, total protein, albumin and urea were also measured. Forage DM intakes were similar for the two grasses. However, because of …


Comparing An Exponential Respiration Model To Alternative Models For Soil Respiration Components In A Canadian Wildfire Chronosequence (Fireresp V1.0), John Zobitz, Heidi Aaltonen, Xuan Zhou, Frank Berninger, Jukka Pumpanen, Kajar Köster Oct 2021

Comparing An Exponential Respiration Model To Alternative Models For Soil Respiration Components In A Canadian Wildfire Chronosequence (Fireresp V1.0), John Zobitz, Heidi Aaltonen, Xuan Zhou, Frank Berninger, Jukka Pumpanen, Kajar Köster

Faculty Authored Articles

Forest fires modify soil organic carbon and suppress soil respiration for many decades after the initial disturbance. The associated changes in soil autotrophic and heterotrophic respiration from the time of the forest fire, however, are less well characterized. The FireResp model predicts soil autotrophic and heterotrophic respiration parameterized with a novel dataset across a fire chronosequence in the Yukon and Northwest Territories of Canada. The dataset consisted of soil incubation experiments and field measurements of soil respiration and soil carbon stocks. The FireResp model contains submodels that consider a Q10 (exponential) model of respiration compared to models of heterotrophic respiration …


A Missense Mutation In Pitx2 Leads To Early-Onset Glaucoma Via Nrf2-Yap1 Axis., Yeming Yang, Xiao Li, Jieping Wang, Junkai Tan, Bernie Fitzmaurice, Patsy M. Nishina, Kuanxiang Sun, Wanli Tian, Wenjing Liu, Xuyang Liu, Bo Chang, Xianjun Zhu Oct 2021

A Missense Mutation In Pitx2 Leads To Early-Onset Glaucoma Via Nrf2-Yap1 Axis., Yeming Yang, Xiao Li, Jieping Wang, Junkai Tan, Bernie Fitzmaurice, Patsy M. Nishina, Kuanxiang Sun, Wanli Tian, Wenjing Liu, Xuyang Liu, Bo Chang, Xianjun Zhu

Faculty Research 2021

Glaucoma is a leading cause of blindness, affecting 70 million people worldwide. Owing to the similarity in anatomy and physiology between human and mouse eyes and the ability to genetically manipulate mice, mouse models are an invaluable resource for studying mechanisms underlying disease phenotypes and for developing therapeutic strategies. Here, we report the discovery of a new mouse model of early-onset glaucoma that bears a transversion substitution c. G344T, which results in a missense mutation, p. R115L in PITX2. The mutation causes an elevation in intraocular pressure (IOP) and progressive death of retinal ganglion cells (RGC). These ocular phenotypes recapitulate …


Non-Compliance To Social Distancing During Covid-19 Pandemic: A Comparative Cross-Sectional Study Between The Developed And Developing Countries, Faiqa Binte Aamir, Syeda Maria Ahmad Zaidi, Saadia Abbas, Syed Roohan Aamir, Syed Nauman Ahmad Zaidi, Karishma Kanhya Lal, Syeda Sadia Fatima Oct 2021

Non-Compliance To Social Distancing During Covid-19 Pandemic: A Comparative Cross-Sectional Study Between The Developed And Developing Countries, Faiqa Binte Aamir, Syeda Maria Ahmad Zaidi, Saadia Abbas, Syed Roohan Aamir, Syed Nauman Ahmad Zaidi, Karishma Kanhya Lal, Syeda Sadia Fatima

Medical College Documents

Background: Despite mass vaccination campaigns, the world has seen a steady rise in the number of SARS-CoV-2 cases, with 178,765,626 cases and 3,869,994 COVID-19 related deaths by June 19th, 2021. Therefore, it is important to enforce social distancing to control its spread. With the variation observed in the severity of the pandemic in different countries, it is also imperative to study the social distancing behaviors amongst the population in developed and developing countries.
Design and Methods: In this cross-sectional study, a total of 384 participants from 14 different countries were surveyed via an online REDCap form.
Results: In …


Application Of Gold Nanoshells For Photothermal Therapy In Lung Cancer Cells, Yva K. Luc Oct 2021

Application Of Gold Nanoshells For Photothermal Therapy In Lung Cancer Cells, Yva K. Luc

USF Tampa Graduate Theses and Dissertations

Lung cancer is the leading cause of cancer deaths in the world. For the year 2020, it was estimated 1.8 million deaths were due to lung cancer alone, making it more lethal than breast cancer and prostate cancer combined. For the year 2021, it is estimated 235,760 new cases of lung cancer and 50% will lead to death. The primary cause of lung cancer is due to cigarette smoking, which accounts for 80% of all lung cancer deaths. Lung cancer screening relies on only different methods, biopsy, bronchoscopy, ultrasound, chest x-ray film, and sputum cytology. These techniques are used to …


Investigation Of An Alternative Protocol For The Production Of Sars-Cov-2 Antigenic Proteins, Nichole Ninaltowski Oct 2021

Investigation Of An Alternative Protocol For The Production Of Sars-Cov-2 Antigenic Proteins, Nichole Ninaltowski

USF Tampa Graduate Theses and Dissertations

With the COVID-19 pandemic showing no signs of slowing down, large-scale antigenic protein production is still needed for surveillance using serologic assays. From screening to vaccines to biotherapeutics, being able to produce the proteins for these assays is essential; however, the current gold standard method for producing SARS-CoV-2 spike proteins is prohibitively expensive for most research groups.

Alternative methods of transfecting mammalian cells to produce recombinant proteins that are relatively inexpensive have been used for years. Unlike the expensive, commercially available lipid-based methods, other established methods such as polyethyleneimine (PEI), are considerably easier, and cheaper to meet the needs of …


A Functional Characterization Of The Omega (Ω) Subunit Of Rna Polymerase In Staphylococcus Aureus, Shrushti B. Patil Oct 2021

A Functional Characterization Of The Omega (Ω) Subunit Of Rna Polymerase In Staphylococcus Aureus, Shrushti B. Patil

USF Tampa Graduate Theses and Dissertations

In bacteria, RNA polymerase (RNAP) is a well-characterized and highly conserved multi-subunit enzyme complex responsible for transcription of DNA into RNA. The ω subunit (rpoZ/RpoZ), one of the smaller accessory subunits of RNAP, is often overlooked and under-studied, however, particularly in Gram-positive species. We have previously shown that deleting ω impacts the integrity of RNA polymerase in S. aureus, especially the β' subunit, and alters preference of the core enzyme for sigma factors, skewing heavily towards σB instead of the housekeeping σ factor. Consequently, this causes deregulation of myriad transcriptional processes, strongly rewiring gene expression circuits, and ultimately impairing the …