Life Sciences Commons^™

Methodology To Evaluate Forage Legumes For Oversowing Grasslands In The Basaltic Region Of Uruguay, D. Real, J. Franco, J. Crossa

IGC Proceedings (1977-2023)

A methodology used to evaluate around 300 temperate and subtropical forage legumes for oversowing the native grasslands of the Basaltic Region of Uruguay is presented in a four-year plan using the minimum amount of seeds per accession and resources. Row-column experimental designs are used to reduce the error variance existing due to the large soil heterogeneity intrinsic to the Region. The ability of the species to grow and reproduce was measured and adjusted least square means were estimated to rank them. Cluster analysis was also useful to group species with similar behaviour overall traits. Preliminary results for the temperate species …

Seasonal Variation Of White Clover Morphology At The North Of Buenos Aires Province, Argentine, E. M. Pagano, Jorge O. Scheneiter

IGC Proceedings (1977-2023)

In order to study the seasonal variation in morphological characters that contribute to forage production in white clover (Trifolium repens L.) cultivars, a trial was conducted at Pergamino Agricultural Experimental Station INTA located north of Buenos Aires province, Argentina. The experimental plots were set out in a completely randomized design with four replicates. Measurements were performed over eight seasons throughout two years. The used cultivars had been previously reported as having differences in seasonal productivity. Significant differences were detected among seasons for all measured characters i.e. stolon length, number of buds, branches and leaves, and leaf area. Differences among …

Stolon Attributes Related To Persistence Of White Clover In Buenos Aires, Argentina, E. M. Pagano, Jorge O. Scheneiter

IGC Proceedings (1977-2023)

A trial was carried out at Pergamino Agricultural Experimental Station (33º 56’ S, 60º 33’ W), Argentina, to study the stolon morphology of white clover cultivars with different leaf size, inflorescence production through reproductive cycle, and vegetative persistence. Treatments were four white clover cultivars: Espanso (Italy), Experimental L.49 (Argentina), Jacui S2 (Brazil) and Nora (Sweden). A completely randomized block design was used (n=6). Throughout two years, measurements of stolon length and dry and organic matter were performed every 60 days. The inflorescence number was also recorded. Results showed significant differences among cultivars in all the studied characters and almost in …

Fish Gelatin Coating As An Antimicrobial Agent On Wild-Caught Louisiana Shrimp, Hunter S. Songy

LSU Master's Theses

Seafood has a short shelf-life because of its unstable nature. Shrimp has a shelf-life of only eight days. Edible coatings, like those made from gelatin, have been used to apply antimicrobial agents to food product surfaces. Organic acids are naturally occurring, low-cost options that have been proven to effectively reduce bacterial loads on food products and extend their shelf-life. Gelatin can be extracted from catfish skin, helping to reduce product waste. The purpose of this study was to determine the potential effectiveness of fish skin gelatin as an antimicrobial coating alone and in combination with lactic acid, and then compare …

The Predictive Influence Of Challenging Behavior On Parent Stress In Young Children With Autism Spectrum Disorder, Paige Weir

LSU Master's Theses

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by deficits in social communication, restricted interest, and repetitive patterns of behavior. Individuals with ASD also exhibit challenging behaviors that affect parent and caregiver stress directly. However, researchers have not yet examined the predictive influence of specific challenging behaviors on parent stress, particularly in young children (i.e., infants and toddlers) with ASD. Therefore, the current study expands existing literature by a) investigating the influence that challenging behaviors of young children with ASD have on parent stress and b) examining the unique contribution that each behavior (i.e., aggressive/disruptive behavior, stereotypy, and self-injurious …

Willingness Of Non-Industrial Private Forest Landowners To Obtain A Private Versus Public Consultation, Erika Largacha

LSU Master's Theses

Promoting conservation practices among family forests landowners play a critical role to enhance forest health and ecosystem services. Several certification programs recognize such efforts, but participation among landowners varies based on their characteristics, program requirements, and incentives. One crucial step of certification is to obtain a forest management plan, but acquisition among landowners remains extremely low. Several studies suggest connecting landowners with foresters as the key element towards the adoption of forest management plans. Because foresters are typically employed by public forestry agencies or as private self-employed consultants, landowners' willingness to work with either may differ. As far as we …

Observation Of Root Rot And Internal Breakdown Of Redclover (Trifolium Pratense L.) In The 3rd Year, H. Nakashima, H. Morishita, T. Hirata, K. Yoshida

IGC Proceedings (1977-2023)

Two red clover (Trifolium pratense L.) cultivars; Hokuseki and Merviot were surveyed growth habits and deterioration at the crown and taproot in the 3rd year. Each cutting plants were marked and measured the plant height and dry matter weight. After 3rd cutting of the 3rd year, plants were digged out and scored the degree of root rot and internal break down (no symptom: 0-severe damaged: 5). The severe damaged plants at the 3rd cutting tend to have larger plant size at the 1st cutting. And other root development traits during growth were observed.

Amphicarpy In The Tropical Legume Centrosema Rotundifolium: A Research Project In Eastern Venezuela, S. Müller, R. Schultze-Kraft, I. Rodríguez

IGC Proceedings (1977-2023)

Amphicarpy is a particular reproduction mechanism by which a plant can produce both above-ground and below-ground seeds and thus has the potential to contribute to an enhanced persistence of a plant population. It can be found in a range of tropical legumes, e.g. in several Centrosema species. The balance between above- and below-ground seed production is evidently influenced by environment and management factors but these influences are not well known. In the case of perennial tropical legumes, in addition to seed production shifts the allocation of resources affects also the production of tuberous roots as storage organs. A research project …

Rooted Plantlet Production In A Vegetatively Reproductive Red Clover (Trifolium Pratense L.) Cv. Astred, M. G. Hyslop, Peter D. Kemp, J. Hodgson

IGC Proceedings (1977-2023)

Vegetatively reproductive cultivars of red clover (Trifolium pratense L.) can produce clonal daughter plantlets under certain management and environmental conditions, which may improve sward persistency. Six trials involving spaced plants, pure swards or grazed mixed swards were conducted near Massey University, Palmerston North, New Zealand, from 1995 to 1998. Rooted plantlets counted in autumn of each year ranged from 5.8±1.6 to 43±5.1 rooted plantlets/parent plant for ungrazed spaced plants, and 0 to 1.8 rooted plantlets/parent plant for pure and mixed swards under grazing management. It is concluded that clonal, rooted plantlet production is highly variable in Astred depending on …

Protein Kinase A And 59 Amp-Activated Protein Kinase Signaling Pathways Exert Opposite Effects On Induction Of Autophagy In Luteal Cells, Emilia Przygrodzka, Corrine F. Monaco, Michele R. Plewes, Guojuan Li, Jennifer R. Wood, Andrea Cupp, John S. Davis

Department of Animal Science: Faculty Publications

In the absence of pregnancy the ovarian corpus luteum undergoes regression, a process characterized by decreased production of progesterone and structural luteolysis involving apoptosis. Autophagy has been observed in the corpus luteum during luteal regression. Autophagy is a self-degradative process important for balancing sources of cellular energy at critical times in development and in response to nutrient stress, but it can also lead to apoptosis. Mechanistic target of rapamycin (MTOR) and 5′ AMP-activated protein kinase (AMPK), key players in autophagy, are known to inhibit or activate autophagy, respectively. Here, we analyzed the signaling pathways regulating the initiation of autophagy in …

Sampling Bias Exaggerates A Textbook Example Of A Trophic Cascade, Elaine M. Brice, Eric J. Larsen, Daniel R. Macnulty

Aspen Bibliography

Understanding trophic cascades in terrestrial wildlife communities is a major challenge because these systems are difficult to sample properly. We show how a tradition of non-random sampling has confounded this understanding in a textbook system (Yellowstone National Park) where carnivore [Canis lupus (wolf)] recovery is associated with a trophic cascade involving changes in herbivore [Cervus canadensis (elk)] behaviour and density that promote plant regeneration. Long-term data indicate a practice of sampling only the tallest young plants overestimated regeneration of overstory aspen (Populus tremuloides) by a factor of 4–7 compared to random sampling because it favoured plants taller than the preferred …

Editorial: Recent Advances In Cardiotoxicity Testing, Tamer M. A. Mohamed, Javid Moslehi, Jonathan Satin

Physiology Faculty Publications

No abstract provided.

Breastfeeding And Risk Of Overweight In Childhood And Beyond: A Systematic Review With Emphasis On Sibling-Pair And Intervention Studies, Kathryn G Dewey, Darcy Güngör, Sharon M Donovan, Emily M Madan, Sudha Venkatramanan, Teresa A Davis, Ronald E Kleinman, Elsie M Taveras, Regan L Bailey, Rachel Novotny, Nancy Terry, Gisela Butera, Julie Obbagy, Janet De Jesus, Eve Stoody

Children’s Nutrition Research Center Staff Publications

BACKGROUND: Breastfeeding is associated with a lower risk of subsequent overweight or obesity, but it is uncertain whether this is a causal relation because most studies have not adequately reduced risk of bias due to confounding.

OBJECTIVES: The aim of this review was to examine whether 1) ever compared with never consuming human milk and 2) different durations of human milk consumption among infants fed human milk are related to later risk of overweight or obesity, with emphasis on sibling-pair and intervention studies.

METHODS: The 2020 Dietary Guidelines Advisory Committee, together with the Nutrition Evidence Systematic Review team, conducted a …

Physical Activity And Fat-Free Mass During Growth And In Later Life, Klaas R Westerterp, Yosuke Yamada, Hiroyuki Sagayama, Philip N Ainslie, Lene F Andersen, Liam J Anderson, Lenore Arab, Issaad Baddou, Kweku Bedu-Addo, Ellen E Blaak, Stephane Blanc, Alberto G Bonomi, Carlijn V C Bouten, Pascal Bovet, Maciej S Buchowski, Nancy F Butte, Stefan G J A Camps, Graeme L Close, Jamie A Cooper, Sai K Das, Richard Cooper, Lara R Dugas, Ulf Ekelund, Sonja Entringer, Terrence Forrester, Barry W Fudge, Annelies H Goris, Michael Gurven, Catherine Hambly, Asmaa El Hamdouchi, Marije B Hoos, Sumei Hu, Noorjehan Joonas, Annemiek M Joosen, Peter Katzmarzyk, Kitty P Kempen, Misaka Kimura, William E Kraus, Robert F Kushner, Estelle V Lambert, William R Leonard, Nader Lessan, Corby K Martin, Anine C Medin, Erwin P Meijer, James C Morehen, James P Morton, Marian L Neuhouser, Theresa A Nicklas, Robert M Ojiambo, Kirsi H Pietiläinen, Yannis P Pitsiladis, Jacob Plange-Rhule, Guy Plasqui, Ross L Prentice, Roberto A Rabinovich, Susan B Racette, David A Raichlen, Eric Ravussin, Rebecca M Reynolds, Susan B Roberts, Albertine J Schuit, Anders M Sjödin, Eric Stice, Samuel S Urlacher, Giulio Valenti, Ludo M Van Etten, Edgar A Van Mil, Jonathan C K Wells, George Wilson, Brian M Wood, Jack Yanovski, Tsukasa Yoshida, Xueying Zhang, Alexia J Murphy-Alford, Cornelia U Loechl, Amy H Luke, Herman Pontzer, Jennifer Rood, Dale A Schoeller, William W Wong, John R Speakman

Children’s Nutrition Research Center Staff Publications

BACKGROUND: Physical activity may be a way to increase and maintain fat-free mass (FFM) in later life, similar to the prevention of fractures by increasing peak bone mass.

OBJECTIVES: A study is presented of the association between FFM and physical activity in relation to age.

METHODS: In a cross-sectional study, FFM was analyzed in relation to physical activity in a large participant group as compiled in the International Atomic Energy Agency Doubly Labeled Water database. The database included 2000 participants, age 3-96 y, with measurements of total energy expenditure (TEE) and resting energy expenditure (REE) to allow calculation of physical …

Metastatic Mat Phenotype Driven By Noncanonical Epha2 Signaling In Melanoma, Chao Zhang

USF Tampa Graduate Theses and Dissertations

Acquired BRAF/MAPK/extracellular signal‒regulated kinase inhibitor resistance in melanoma results in a new transcriptional state associated with an increased risk of metastasis. In this study, we identified noncanonical ephrin receptor (Eph) EphA2 signaling as a driver of the resistance-associated metastatic state. We used mass spectrometry‒based proteomic and phenotypic assays to demonstrate that the expression of active noncanonical EphA2-S897E in melanoma cells led to a mesenchymal-to-amoeboid transition driven by Cdc42 activation. The induction of mesenchymal-to-amoeboid transition promoted melanoma cell invasion, survival under shear stress, adhesion to endothelial cells under continuous-flow conditions, increased permeability of endothelial cell monolayers, and stimulated melanoma transendothelial cell …

Ecology And Diversity Of Boletes Of The Southeastern United States, Arian Farid

USF Tampa Graduate Theses and Dissertations

The boletes are conspicuous stipito-pileate macrofungi that are generally defined by their tubulose hymenophores. Molecular phylogenetic analyses have informed extensive taxonomic revisions across the boletes. Despite this, the boletes in the southeastern USA have been largely absent from the literature. This dissertation seeks to understand the microbiome inhabiting the tuberculate ectomycorrhizae of the bolete Hemileccinum rubropunctum, and addresses taxonomic revisions of boletes found in the Boletaceae and Suillaceae. The introduction provides a brief background on boletes, as well as our understanding of tuberculate ectomycorrhizae. The second chapter seeks to understand the composition of the microbiome tuberculate ectomycorrhizae of H. rubropunctum …

The Temperature-Dependent Conformational Ensemble Of Sars-Cov-2 Main Protease (Mpro), Ali Ebrahim, Blake T. Riley, Desigan Kumaran, Babak Andi, Martin R. Fuchs, Sean Mcsweeney, Daniel A. Keedy

Publications and Research

The COVID-19 pandemic, instigated by the SARS-CoV-2 coronavirus, continues to plague the globe. The SARS-CoV-2 main protease, or M^pro, is a promising target for development of novel antiviral therapeutics. Previous X-ray crystal structures of M^pro were obtained at cryogenic temperature or room temperature only. Here we report a series of high-resolution crystal structures of unliganded M^pro across multiple temperatures from cryogenic to physiological, and another at high humidity. We interrogate these datasets with parsimonious multiconformer models, multi-copy ensemble models, and isomorphous difference density maps. Our analysis reveals a temperature-dependent conformational landscape for M^pro, including …

The Dynamics Of Dominance: Open Questions, Challenges And Solutions, Eli D. Strauss, Daizaburo Shizuka

School of Biological Sciences: Faculty Publications

Although social hierarchies are recognized as dynamic systems, they are typically treated as static entities for practical reasons. Here, we ask what we can learn from a dynamical view of dominance, and provide a research agenda for the next decades. We identify five broad questions at the individual, dyadic and group levels, exploring the causes and consequences of individual changes in rank, the dynamics underlying dyadic dominance relationships, and the origins and impacts of social instability. Although challenges remain, we propose avenues for overcoming them. We suggest distinguishing between different types of social mobility to provide conceptual clarity about hierarchy …

Pi3k Pathway Inhibition With Nvp-Bez235 Hinders Glycolytic Metabolism In Glioblastoma Multiforme Cells, Shreya Udawant, Carl Litif, Alma Lopez, Bonnie Gunn, Erin Schuenzel, Megan Keniry

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Glioblastoma (GBM) is the most lethal primary brain cancer that lacks effective molecular targeted therapies. The PI3K/AKT/mTOR pathway is activated in 90% of all Glioblastoma multiforme (GBM) tumors. To gain insight into the impact of the PI3K pathway on GBM metabolism, we treated U87MG GBM cells with NVP-BEZ235 (PI3K and mTOR a dual inhibitor) and identified differentially expressed genes with RNA-seq analysis. RNA-seq identified 7803 differentially regulated genes in response to NVP-BEZ235. Gene Set Enrichment Analysis (GSEA) identified two glycolysis-related gene sets that were significantly enriched (p < 0.05) in control samples compared to NVP-BEZ235-treated samples. We validated the inhibition of glycolytic genes by NVP-BEZ235 and examined the impact of the FOXO1 inhibitor (AS1842856) on these genes in a set of GBM cell lines. FOXO1 inhibition alone was associated with reduced LDHA expression, but not ENO1 or PKM2. Bioinformatics analyses revealed that PI3K-impacted glycolytic genes were over-expressed and co-expressed in GBM clinical samples. The elevated expression of PI3K-impacted glycolytic genes was associated with poor prognosis in GBM based on Kaplan–Meier survival analyses. Our results suggest novel insights into hallmark metabolic reprogramming associated with the PI3K-mTOR dual inhibition.

Selecting Crimson Clover For Hard Seed And Late Maturity, G. W. Evers, G. R. Smith

IGC Proceedings (1977-2023)

Available crimson clover (Trifolium incarnatum L.) cultivars in the USA flower approximately 2 weeks before annual ryegrass (Lolium multiflorum L.) which makes it difficult to manage a mixture of the two for reseeding. The objective of this study is the development of a crimson clover cultivar that flowers at approximately the same time as annual ryegrass and has a high level of hard seed to enhance reseeding. Selections for late maturity were made out of >Chief= crimson clover. After two cycles of selection for later maturity and one cycle for hard seed, nineteen half-sib lines were identified that …

A Novel Technique To Produce Polygenic Resistance To Anthracnose In Stylosanthes Capitata, Bela Grof, Celso D. Fernandes, A. T. F. Fernandes

IGC Proceedings (1977-2023)

An improved cultivar, based on 17 genotypes of S. capitata and six of S.macrocephala, was developed at the Embrapa Beef Cattle Research Center, Campo Grande, Brazil. The aim was to create durable, race non-specific resistance to anthracnose controlled by polygenic factors. A mass hybridisation technique was employed to produce a high degree of genetic diversity and sizeable quantities of seed of hybrid-derived progenies of Brazilian and Venezuelan genotypes of S. capitata. Outcrossing resulted in a significant improvement in the forage production of progeny of Venezuelan accessions. The multicross was evaluated in multilocational trials, each representing a large tract …

Genetic Resources For Tropical Areas: Achievements And Perspectives, Cacilda B. Do Valle

IGC Proceedings (1977-2023)

This paper analyses the present status of tropical forage resources and their utilization in the development of novel cultivars based on the accumulated information from past decades of collecting, evaluation and whatever little breeding has been pursued. The situation of world collections of tropical forages is presented and discussed in the light of limited investment and dwindling resources. A cause for concern is the lesser priority assigned to conservation and manipulation of official tropical germplasm banks, such as CSIRO’S and CIAT’s. In order to assure the availability of tropical genetic resources for the future it is imperative that international efforts …

Genetic Resources For Temperate Areas - Achievements And Perspectives, Mónica Rebuffo, T. Abadie

IGC Proceedings (1977-2023)

The processes of natural evolution and domestication of temperate forage genetic resources resulted in a build-up of genetic diversity, being remarkable in natural populations of grasses and legumes from Central Europe and the Mediterranean basin. Worldwide utilization of few species caused further increment in intraspecific genetic differentiation, meanwhile landraces became adapted to local agricultural production systems. Highlights are given to the risk of genetic erosion of indigenous grasslands as a consequence of the ecosystem modifications imposed by agriculture, as well as the drastic reduction of intraspecific genetic diversity caused by the process of plant breeding and environmental leveling in modern …

Response Of Contrasting Cocksfoot Varieties To Summer Moisture Availability In A Mediterranean Environment, E. Piano, M. Romani, A. M. Carroni, L. Pecetti

IGC Proceedings (1977-2023)

The responsiveness to water availability in summer and the effect of summer drought were assessed in a Mediterranean environment on both temperate and Mediterranean cocksfoot (Dactylis glomerata L.) materials. Three consecutive evaluation phases were contemplated: i) under rainfed conditions from autumn to spring; ii) under contrasting moisture conditions in the following summer; and iii) under rainfed conditions in the subsequent autumn-spring season to assess the effect of the previous summer treatments. The ability to become summer dormant under conducive conditions, such as in Mediterranean germplasm, proved a plant pre-requisite for survival under drought. All the “summer-active”, temperate varieties did …

Scaling Of Computed Tomography Body Composition To Height: Relevance Of Height-Normalized Indices In Patients With Colorectal Cancer, Justin C. Brown, Steven B. Heymsfield, Bette J. Caan

School of Medicine Faculty Publications

Background: Body weight scales to height with a power of ≈2 (weight/height2), forming the basis of body mass index (BMI). The corresponding scaling of body composition measured by abdominal computed tomography (CT) to height has not been established. The objective of this analysis was to quantify the scaling of body composition measured by a single-slice axial abdominal CT image (skeletal muscle, and visceral, subcutaneous, and total adipose tissue) to height in patients with colorectal cancer (CRC). Methods: This cross-sectional study included non-Hispanic white males and females, aged 18–80 years, who were diagnosed with stage I–III CRC at an integrated health …

Short-Term Waterlogging Depresses Early Growth Of Sunflower (Helianthus Annuus L.) On Saline Soils With A Shallow Water Table In The Coastal Zone Of Bangladesh, Priya Lal Chandra Paul, Richard W. Bell, Edward G. Barrett-Lennard, Enamul Kabir, Mohammed Mainuddin, Khokan Kumer Sarker

Grain and Other Field Crops Research Articles

Sunflower (Helianthus annuus L.), which is widely grown globally for its high-quality edible oil, is reasonably salt and drought tolerant but it is susceptible to waterlogging. In the saline coastal zone of the Ganges delta, sunflower is often exposed to sudden heavy rainfall during early growth but plant tolerance to such events is not known. Hence, we evaluated the effect of short-term soil inundation (referred to as waterlogging) for 0, 24, 48 and 72 h on sunflower at emergence, 2-leaf, and 4-leaf stages in early- and late-sown crops under field conditions (saline, clay-textured soil, and shallow groundwater). Waterlogging …

Forage Production And Nutritive Value Of Four Promising Sorghum X Sudangrass Hybrid In Korea, S. Seo, J. G. Kim, E. S. Chung, J. K. Lee, S. H. Yoon

IGC Proceedings (1977-2023)

Sorghum x sudangrass hybrid (Sorghum bicolor (L.) Moench) is one of the most important annual grass utilized for supplemental summer forage. There were 14 National Livestock Cooperative Federation (NLCF)’s recommended forage sorghum´sudangrass hybrids in Korea. Among them several hybrids were produced widely, however, they have not been evaluated under same environmental conditions. Therefore, a field experiment was carried out to compare the plant height, forage yield and nutritive value of the NLCF’s recommended cultivars at NLRI, Suwon, Korea in 1995. The four promising cultivars of sorghum´sudangrass hybrid used in this study were P 988, TE-Haygrazer, NC+ 855 (heading type), …

The Effect Of Plant Population On The Yield And Quality Of Annual Rye-Grass, M. E. V. L. Lourenço, P. M. M. Palma

IGC Proceedings (1977-2023)

The primary objectives of this study were to evaluate the effect of three plant population levels (350, 750 and 1150 plants m^-2) on dry matter yield and forage quality (crude protein and dry matter digestibility) of four rye-grass genotypes (Barspectra, Billion, Clipper and Pollanum) used in two harvests (March and May).

The results for dry matter yield means by year, genotype, and harvest were always higher in the second harvest than in the first, and the highest total mean value was reached in the first year (5853 Kg ha^-1). The genotype Billion was the most stable …

High Yielding And Disease Resistant Elephant Grass Suitable For Intensive Smallholder Dairy Farmers In Kenya, A. B. Orodho

IGC Proceedings (1977-2023)

In Kenya, over 80% of milk is produced by the smallholder dairy farmers who mainly practice semi or zero-grazing dairy management system. Scarcity of livestock feed is a great limiting factor in the dairy industry, especially in the small scale farming systems which rely mostly on fodder. Elephantgrass (Pennisetum purpureum) is the major livestock feed and its productivity depends mostly on management practices. The current fodder crops grown by farmers have been developed through systematic research of collection/introductions, screening and evaluation of forages for their suitability as livestock feed. These adopted fodder cultivars are undergoing yield reduction due …

Intake And Digestibility Of Black Locust Foliage Fed To Growing Goat Wethers, L. J. Unruh, J-M. Luginbuhl, J. P. Mueller

IGC Proceedings (1977-2023)

Leaves of Black locust (BL, Robinia pseudoacacia), a native southeastern United States tree species known to contain substantial levels of condensed tannins, were fed to 16, four month old (20.4 kg body weight [BW]) Boer wether goats (Capra hircus hircus) to determine their effects on intake and digestibility. Four diets were stall fed in a randomized complete block design (RCBD) with four replications. Diets included: (1) 100% eastern gamagrass (EGH; Tripsacum dactyloides) hay; (2) 70% EGH and 30% mixture of ground corn (GC; Zea mays) and soybean (Glycine max) meal (SBM); (3) 75% …