Life Sciences Commons^™

Anti-Lymphangiogenic Diterpenes From The Bark Of Calocedrus Macrolepis Var. Formosana, Tzong-Huei Lee, Chin-Lin Hsieh, Ho-Cheng Wu, Shih-Wei Wang, Chen-Lin Yu, George Hsiao, Ming-Jen Cheng, Wen-Tsong Hsieh, Yueh-Hsiung Kuo

Journal of Food and Drug Analysis

Eight new diterpenes, 6α,7β-dihydroxyferruginol (1), 6α,7α-dihydroxyferruginol (2), 6α-hydroxyhinokiol (3), 4α-hydroxy-7-oxo-18-norabieta-8,11,13-trien-4α-ol (4a), 15,16-dehydrosugiol (5), 7-methoxy-6,7-secoabieta-8,11,13-triene-6,12-diol (6), 7α-acetoxyabieta-8,12-diene-11,14-dione (7), 7α-butyloxyethyloxyabieta-8,12-diene-11,14-dione (8), along with four known compounds, 6,7-dehydroferruginol (9), 12-hydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial (10), 7α-11-dihydroxy-12-methoxy-8,11,13-abietatriene (11), and 11,14-dihydroxy-8,11,13-abietatrien-7-one (12) were successfully isolated from the bark of Calocedrus macrolepis var. formosana. The structures of all isolates were elucidated by physical data (appearance, UV, IR, optical rotation) and spectroscopic data (1D, 2D NMR, and HREIMS). Compounds 9, 10, 11, and 12 showed promising growth-inhibitory effect on human lymphatic endothelial cells (LECs). Among these compounds, compound 10 exerted the most potent anti-lymphangiogenesis property by suppressing cell growth and …

Medicarpin Isolated From Radix Hedysari Ameliorates Brain Injury In A Murine Model Of Cerebral Ischemia, Chang-Ming Chern, Chung-Kuang Lu, Kuo-Tong Liou, Yea-Hwey Wang, Keng-Chang Tsai, Chia-Lin Chang, Chia-Che Chang, Yuh-Chiang Shen

Journal of Food and Drug Analysis

The development of effective post-stroke therapy is highly demanded. Medicarpin is a key active component of a famous Chinese herbal prescription used for post-stroke treatment in Taiwan; however, little is known about its biological effects and mechanisms of action. Herein, we implemented a murine model of cerebral ischemic/reperfusional injury-related stroke to elucidate medicarpin’s neuroprotective effect. In male ICR mice 24 h after stroke induction, treatment with medicarpin (0.5 and 1.0 mg/kg, i.v.) markedly enhanced the survival rates, improved moving distance and walking area coverage, reduced brain infarction, and preserved the blood-brain barrier, supporting medicarpin’s protective effect on stroke-induced injury. Immunohistochemistry …

In-Silico Efficacy Of Potential Phytomolecules From Ayurvedic Herbs As An Adjuvant Therapy In Management Of Covid-19, Bhanu Kumar, Ankita Misra, Satyendra Pratap Singh, Yogeshwar Vikram Dhar, Poonam Rawat, Debprasad Chattopadhyay, Saroj Kanta Barik, Sharad Srivastava

Journal of Food and Drug Analysis

The recent COVID-19 outbreak caused by SARS-CoV-2 virus has sparked a new spectrum of investigations, research and studies in multifarious directions. Efforts are being made around the world for discovery of effective vaccines/drugs against COVID-19. In this context, Ayurveda, an alternative traditional system of medicine in India may work as an adjuvant therapy in compromised patients. We selected 40 herbal leads on the basis of their traditional applications. The phytomolecules from these leads were further screened through in-silico molecular docking against two main targets of SARS-CoV-2 i.e. the spike protein (S; structural protein) and the main protease (M^{PRO …}

Conducting Polymer-Based Sensors For Food And Drug Analysis, Chia-Hsin Lin, Jia-Hui Lin, Chien-Fu Chen, Yoshihiro Ito, Shyh-Chyang Luo

Journal of Food and Drug Analysis

Conducting polymers (CPs) are a category of polymeric materials with conjugated main chains. The characteristic electrical and optical properties of CPs can be fine-tuned through controlling the doping states of CPs. Because of their long-term stability in water, CPs have been demonstrated as electroactive biointerfaces and electrode materials especially in aqueous environments. Serving as multifunctional interfaces and organic electrodes for the integration bioelectronics and devices, CPs have been studied and applied in various biological applications. This paper provides a review of conducting polymer–based electrochemical sensors, particularly those used in biological fields. General conducting polymers and derivatives and their main electrochemical …

Recent Developments In Sensing Of Oversulfated Chondroitin Sulfate In Heparin. A Review ., Manivannan Madhu, Wei-Lung Tseng

Journal of Food and Drug Analysis

Oversulfated chondroitin sulfate (OSCS), a non-natural sulfated glycosaminoglycan, recognizes as a significant containment in the pharmaceutical heparin, and it could trigger adverse reactions. Chromatography-, electrophoresis-, electrochemistry-, and spectroscopy-related techniques are currently available for accurate and precise analysis of a trace amount of OSCS in heparin. Recently, emerging studies focus on developing colorimetric and fluorescent probes to monitor OSCS containments in heparin. Therefore, this current review aims to describe the sensing principle and procedure of the reported probes that are sensitive and selective toward OSCS in heparin without the interferences of other sulfated glycosaminoglycans. The reported OSCS-specific probes are comprehensively discussed …

Stakeholder Involvement In Upscaling Of Soil Fertility Research Output In Tharaka-Nithi County, Kenya, Serah W. Kimaru-Muchai

Journal of Global Awareness

Food insecurity in Sub-Saharan Africa has prompted a lot of research in the development of soil fertility technologies; however, few of the recommendations from soil fertility management research have been put into use by the target end-users. The objective of the study was to investigate information exchange pathways used by researchers in upscaling of soil fertility in Maara and Mbeere South Sub-counties in Kenya. Structured questionnaires were used to collect information from 22 researchers and 240 farmers. Data was analyzed using descriptive statistics like frequency, mean, and percentages, while Chi-square, Kendal's correlation coefficient was used to test the magnitude of …

Letter From The Editor, Jay Nathan

Journal of Global Awareness

No abstract provided.

Citric Acid/Β-Alanine Carbon Dots As A Novel Tool For Delivery Of Plasmid Dna Into E. Coli Cells, Anju Pandey, Asmita Devkota, Anil Sigdel, Zeinab Yadegari, C. Korsi Dumenyo, Ali Taheri

Agricultural and Environmental Sciences Faculty Research

Successful delivery of plasmid DNA into the microbial cells is fundamental in recombinant DNA technology. Natural bacterial transformation is limited to only certain species due in part to the repulsive forces between negatively charged DNA and bacterial membranes. Most common method of DNA delivery into bacteria is artificial transformation through heat shock and electroporation. These methods require sophisticated instruments and tedious steps in preparation of competent cells. Transformation by conjugation is also not applicable to all plasmids. Nanoparticles have been used successfully in therapeutics for drug delivery into animal cells. They are starting to gain popularity in plant sciences as …

A Link Between Methylglyoxal And Heart Failure During Hiv-1 Infection, Prasanta K. Dasg, Fadhel A. Alomar, Jesse L. Cox, Joellyn Mcmillan, Bryan T. Hackfort, Edward Makarov, Brenda Morsey, Howard S. Fox, Howard E. Gendelman, Santhi Gorantla, Keshore R. Bidasee

Department of Biochemistry: Faculty Publications

Early-onset heart failure (HF) continues to be a major cause of morbidity and mortality in people living with human immunodeficiency virus type one (HIV-1) infection (PLWH), yet the molecular causes for this remain poorly understood. Herein NOD.Cg- Prkdc^scidIl2rg^tm1Wjl/SzJ humanized mice (Hu-mice), plasma from PLWH, and autopsied cardiac tissues from deceased HIV seropositive individuals were used to assess if there is a link between the glycolysis byproduct methylglyoxal (MG) and HF in the setting of HIV-1 infection. At five weeks post HIV infection, Hu-mice developed grade III-IV diastolic dysfunction (DD) with an associated two-fold increase in plasma …

Comparison Of Methods To Estimate Ruminal Degradation And Intestinal Digestibility Of Protein In Hydrolyzed Feather Meal With Or Without Blood, Kassidy Buse, D. L. Morris, H. L. Diaz, O. R. Drehmel, Paul J. Kononoff

Department of Animal Science: Faculty Publications

Hydrolyzed feather meal (HFM) is a feedstuff high in rumen undegraded protein (RUP) that can be used as an effective source of metabolizable protein for dairy cattle. Because the production process may vary, the rumen degradability and intestinal digestibility of HFM may also vary. Additionally, some processes may incorporate additional blood into the final product to result in feather meal with poultry blood. To determine the rumen degradability and intestinal digestibility of these products, several laboratory assays can be used; the common assays are the mobile bag (MOB), modified three-step (MTS), and Ross (ROS) assays. Although all 3 assays determine …

Active Targeting Of Estrogen Receptor (Er)-Positive Breast Cancer Cells Using Nanotechnology, Molly White

Theses and Dissertations

The American Cancer Society has predicted an estimated 281,550 new invasive breast cancer cases for 2021. Chemotherapy is one of the main options available to treat this disease, but it can result in drug resistance and harmful side effects, as traditional methods do not directly target the cancer cells. To address this issue, nano formulations have been widely researched for targeted delivery of anticancer drugs. The primary objective of this project was to develop a novel nanodrug, rapamycin-loaded gold nanorods functionalized with polyethylene glycol and epithelial cell adhesion molecule (EpCAM), to selectively target breast cancer cells. The work involved characterizing …

The Transfer Of Motor Skill Practice To The Game: A Review Of Transfer Of Learning Theory And Applications, Silver Dawson

Honors Theses

Abstract

Transfer of learning from practice to competition is a prevalent issue that needs the assistance of professionals in motor behavior and coaching education. This review provides a theoretical and applied overview of transfer of learning and practice design. Lacking the appropriate knowledge and skillset to create an effective practice design does not have to be common for coaches. Athletes and coaches need more evidence of transfer of learning behind their practice design and assessment. This research review gives support to theories and concepts like Fitts and Posner's stages of learning, specificity of learning, schema theory, verbal cues augmented feedback, …

Pengaruh Cara Pemberian Pupuk Npk Dan Frekuensi Pemberian Paklobutrazol Terhadap Pertumbuhan Dan Hasil Benih Kentang G0 Di Dataran Medium, Jajang Sauman Hamdani, Sumadi, Kusumiyati, Syariful Mubarok

Jurnal Kultivasi

Produksi benih kentang (Solanum tuberosum L) di dataran medium dapat menjadi pilihan untuk menghindari kerusakan lingkungan dan terbatasnya areal untuk produksi benih kentang di dataran tinggi. Salah satu upaya untuk peningkatan produksi benih kentang generasi ke-0 (G0) adalah pemberian pupuk NPK dan zat pengatur tumbuh. Sehubungan hal tersebut, maka perlu dilakukan kajian cara pemberian pupuk NPK dan frekuensi pemberian paklobutrazol yang tepat untuk meningkatkan pertumbuhan dan hasil benih kentang G0 di dataran medium. Percobaan ini bertujuan untuk mengetahui interaksi antara cara pemberian pupuk NPK dan frekuensi pemberian paklobutrazol yang dapat meningkatkan pertumbuhan dan hasil benih kentang G0 di dataran medium. …

Efikasi Campuran Tienkarbazon Metil Dan Tembotrion Sebagai Herbisida Purna Tumbuh Terhadap Gulma Berdaun Lebar Dan Sempit Pada Budidaya Tanaman Jagung, Denny Kurniadie, Uum Umiyati, Devina Alifia Ardhianty

Jurnal Kultivasi

Kompetisi nutrisi akibat kehadiran gulma di area pertanaman jagung (Zea mays L.) dapat menyebabkan kehilangan hasil yang signifikan pada tanaman jagung. Penggunaan campuran herbisida merupakan salah satu upaya dalam mengendalikan gulma setelah tanaman tumbuh serta menghindari pembentukan gulma yang resisten. Tujuan penelitian ini adalah mengetahui pengaruh herbisida Tienkarbazon Metil 68 g/L dan Tembotrion 345 g/L dalam mengendalikan gulma berdaun lebar dan gulma rumput pada pertanaman jagung. Penelitian dilakukan pada bulan Oktober 2020 hingga Januari 2021 di Lahan Percobaan Ciparanje, Fakultas Pertanian, Universitas Padjadjaran, Kampus Jatinangor, Kabupaten Sumedang. Tempat penelitian terletak pada ketinggian ± 752 m di atas permukaan laut. Penelitian …

Keragaman Genetik Koleksi Plasma Nutfah Jewawut Sister Line Dan Lokal Menggunakan Marka Ssr, Karlina Syahruddin, Muhammad Azrai, Marcia Bunga Pabendon, Muhammad Abid, Amin Nur

Jurnal Kultivasi

Keragaman genetik sangat penting dalam pemuliaan tanaman, oleh karena itu informasi genetik sangat dibutuhkan dalam proses seleksi. Teknologi molekuler dengan menggunakan marka Simple Sequence Repeat (SSR) saat ini banyak digunakan karena keakuratan informasi dan polimorfik untuk spesies atau galur yang berkerabat dekat. Penelitian ini dilakukan untuk mengidentifikasi keragaman genetik dari 14 koleksi plasmanutfah jewawut berkerabat dekat dengan menggunakan 27 marka SSR yang menyebar pada semua kromosom jewawut. Hasil penelitian menunjukkan bahwa koleksi plasmanutfah jewawut berkerabat dekat mengelompok pada klaster yang sama, kecuali pada koleksi aksesi Wete dari Nusa Tenggara Timur (NTT) yang menyebar pada kelompok yang berbeda pada kisaran nilai …

Impact Of Theory-Based Interventions On Meaningfulness Of Undergraduate Nursing Pharmacology Student Learning, Betsy L. Mauldin

Nursing Theses and Dissertations

Objective: Nursing pharmacology is a complex and content-dense course, often cited as difficult for nurse educators and students and a reason for not progressing in nursing curricula. Even nurses who experience high academic performance report feeling ill-prepared to adequately perform medication management for nursing practice in the current fast-paced environment. The objective of this five-chapter portfolio is to explain the development and implementation of a two-pronged intervention to facilitate meaningful learning of pharmacology principles within nursing students. Chapter 1 describes an overview of the approach to the intervention.

Methods: Chapter 2 describes one prong of the study intervention, the Pharmacology …

Biodiversity And Fire In Shortgrass Steppe, Paulette L. Ford

IGC Proceedings (1977-2023)

Effects of fire at two levels of intensity on beetle diversity in shortgrass steppe were examined. The experimental design was completely randomized, with 3 treatments and 4 replicates per treatment. Treatments were two levels of fire 1) dormant-season fire (relatively hot), and 2) growing-season fire (relatively cool), and unburned plots. The response variables were arthropod species richness and abundance. The intermediate disturbance hypothesis predicts that maximum species richness occurs at intermediate levels of disturbance. Data obtained in this study support that prediction. Species richness was higher on plots of intermediate fire intensity than the more intensely burned plots, and almost …

Mechanization: Planning And Selection Of Equipment, C. A. Rotz

IGC Proceedings (1977-2023)

The planning and selection of equipment for harvest and handling of forage crops can greatly impact the performance and profitability of a farm. The type and size of equipment used affects the harvested yield and nutritive value of the forage crop as well as production costs. Through interactions with other parts of the farm, these effects can impact market value of the forage, animal intake and performance, delays in other farm operations, other production costs, and ultimately farm profit. Standard procedures and relationships have been developed for determining the most appropriate equipment for a given production system. Because of the …

Conserved Forage (Silage And Hay): Progress And Priorities, R. E. Muck, K. J. Shinners

IGC Proceedings (1977-2023)

Forage conservation permits a better supply of quality feed when forage production is low. While haymaking and ensiling have been practiced for generations, research is still needed to 1) understand the processes affecting quality during harvesting and storage and 2) develop practical means to minimize losses and enhance quality.

Several trends in harvesting forages for silage are notable. Kernel processing of maize, once confined to Europe, has become popular in North America. Self-propelled forage harvesters have a larger share of the market due to more contract harvesting and larger farms. Larger harvesters, rakes and mergers help improve productivity and forage …

Let There Be Light: Restoring Vision With Optogenetics, Kristin Woodard

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

Nuclear Pore Protein Nup210 Depletion Suppresses Metastasis Through Heterochromatin-Mediated Disruption Of Tumor Cell Mechanical Response., Ruhul Amin, Anjali Shukla, Jacqueline Jufen Zhu, Sohyoung Kim, Ping Wang, Simon Zhongyuan Tian, Andy D Tran, Debasish Paul, Steven D Cappell, Sandra Burkett, Huaitian Liu, Maxwell P Lee, Michael J Kruhlak, Jennifer E Dwyer, R Mark Simpson, Gordon L Hager, Yijun Ruan, Kent W Hunter

Faculty Research 2021

Mechanical signals from the extracellular microenvironment have been implicated in tumor and metastatic progression. Here, we identify nucleoporin NUP210 as a metastasis susceptibility gene for human estrogen receptor positive (ER+) breast cancer and a cellular mechanosensor. Nup210 depletion suppresses lung metastasis in mouse models of breast cancer. Mechanistically, NUP210 interacts with LINC complex protein SUN2 which connects the nucleus to the cytoskeleton. In addition, the NUP210/SUN2 complex interacts with chromatin via the short isoform of BRD4 and histone H3.1/H3.2 at the nuclear periphery. In Nup210 knockout cells, mechanosensitive genes accumulate H3K27me3 heterochromatin modification, mediated by the polycomb repressive complex 2 …

Effect Of Music On Rating Of Perceived Exertion And Duration Of Physical Activity, Mia Hines

Honors Projects

This study addresses the relationship between listening to music and physical activity.

Origin And Evolution Of Hiv-1 Subtype A6, Syed Hani Abidi, Lazzat Aibekova, Salima Davlidova, Aidana Amangeldiyeva, Brian Foley, Syed Ali

Department of Biological & Biomedical Sciences

Background: HIV outbreaks in the Former Soviet Union (FSU) countries were characterized by repeated transmission of the HIV variant AFSU, which is now classified as a distinct subtype A sub-subtype called A6. The current study used phylogenetic/phylodynamic and signature mutation analyses to determine likely evolutionary relationship between subtype A6 and other subtype A sub-subtypes.
Methods: For this study, an initial Maximum Likelihood phylogenetic analysis was performed using a total of 553 full-length, publicly available, reverse transcriptase sequences, from A1, A2, A3, A4, A5, and A6 sub-subtypes of subtype A. For phylogenetic clustering and signature mutation analysis, a total of 5961 …

Inflammatory Mediation Of Heat Stress-Induced Growth Deficits In Livestock And Its Potential Role As A Target For Nutritional Interventions: A Review, Micah S. Most, Dustin T. Yates

Department of Animal Science: Faculty Publications

Heat stress is detrimental to well-being and growth performance in livestock, and systemic inflammation arising during chronic heat stress contributes to these poor outcomes. Sustained exposure of muscle and other tissues to inflammation can impair the cellular processes that facilitate muscle growth and intramuscular fat deposition, thus reducing carcass quality and yield. Climate change is expected to produce more frequent extreme heat events, increasing the potential impact of heat stress on sustainable livestock production. Feedlot animals are at particularly high risk for heat stress, as confinement limits their ability to seek cooling from the shade, water, or breeze. Economically practical …

2021 Seedless Pickling Cucumber Variety Trial, Ben Phillips, Jessica Good

Midwest Vegetable Trial Reports

A pickling cucumber variety trial was planted at the Saginaw Valley Research and Extension Center (43.399097, -83.694497, Frankenmuth, Michigan). Bejo (BJ), Nunhems (NU), Rijk Zwaan (RZ), and Sakata (SK) seed companies donated parthenocarpic (seedless) varieties for mechanical once-over harvest. The top five varieties each had Total Percent Recovery scores of 100%. When arranged in order of their highest combined clean yields of the most profitable fruit sizes (2B + 3A + 3B), they were NU 2106 (711 bu/a), NU 2105 (699 bu/a), RZ 16 (639 bu/a), RZ 11 (446 bu/a), and BJ Aristan (303 bu/a).

Visualizing Phytochemical-Protein Interaction Networks: Momordica Charantia And Cancer, Yumi L. Briones, Alexander T. Young, Fabian M. Dayrit, Armando Jerome De Jesus, Nina Rosario L. Rojas

Chemistry Faculty Publications

The in silico study of medicinal plants is a rapidly growing field. Techniques such as reverse screening and network pharmacology are used to study the complex cellular action of medicinal plants against disease. However, it is difficult to produce a meaningful visualization of phytochemical-protein interactions (PCPIs) in the cell. This study introduces a novel workflow combining various tools to visualize a PCPI network for a medicinal plant against a disease. The five steps are 1) phytochemical compilation, 2) reverse screening, 3) network building, 4) network visualization, and 5) evaluation. The output is a PCPI network that encodes multiple dimensions of …

Dolphins In Ny? Researchers Turn To Boat Tours For Sightings, Aaron K. Tremper

Capstones

Before 2009, coastal bottlenose dolphins were a rare sight north of New Jersey. According to local researchers, large pods of bottlenose dolphins made their way that spring/summer along Long Island. While dolphin population data by state and federal wildlife regulators are spotty, studies by local scientists suggest more dolphins have been flocking to New York since 2009. Many researchers are turning to whale-watching ventures to learn more about what dolphins are up to in the Big Apple.

https://jkt711.github.io/capstone/

Lost At Sea, Anny Oberlink

Capstones

At the end of World War I and World War II, in a new era of peace, nations confronted an unprecedented logistical problem: millions of tons of unexploded ordnance—once a wartime boon—had become a peacetime burden. Faced with a mandate to dispose of excess munitions, militaries turned to dumping their stockpiles into the sea. But now a complex and urgent issue is emerging. Increasingly, as industry looks to build offshore—wind power turbines, internet cables, oil pipelines—they are facing a potential peril: millions of tons of unexploded bombs and ammunition that are lying on the ocean floor can explode or leak …

Sediment Budgets For Small Salinized Agricultural Catchments In Southwest Australia And Implications For Phosphorus Transport, Robert J. Wasson, David Weaver

Natural Resources Research Articles

Examples of sediment budgets are needed to document the range of budget types and their controls. Sediment budgets for three small agricultural catchments (7.6 to 15.6 km2) in southwestern Australia are dominated by channel and gully erosion, with sheet and rill erosion playing a subordinate role. Erosion was increased by clearing naturally swampy valley floors and hillslopes for agriculture and grazing, and episodic intense rainstorms. The proportion of sediment from channel and gully erosion in the sediment budget appears to be determined by the depth of alluvial fills. Dryland salinization caused by clearing native vegetation has connected hillslopes to channels …

Comparison Of Quality And Production By Different Silage Making Methods Of Barley Cultivated In The Paddy After Rice Harvest, J. S. Shin, E. B. Yoon, M. S. Park

IGC Proceedings (1977-2023)

The objective of this study was to compare whether the productivity and production cost of whole crop barley was affected by silage making methods at early bloom stage in the rice field. In nutritive value, quality and palatability of silage, baled silage making method was a little higher than that of crushed silage making. On the other hand, in terms of dry matter and total digestible nutrient (TDN) production cost, proper work capacity at about 15 ha or more was desirable when making baled silage. In this case, the estimated dry matter production cost and TDN cost per ha was …