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Articles 58621 - 58650 of 255948

Full-Text Articles in Life Sciences

Weedy Rice As A Model System For The Study Of Microevolutionary Interactions In Agricultural Contexts, Marshall Jon Wedger Dec 2021

Weedy Rice As A Model System For The Study Of Microevolutionary Interactions In Agricultural Contexts, Marshall Jon Wedger

Arts & Sciences Graduate Student Theses and Dissertations

Just under one-half of the global population relies on cultivated rice (Oryza sativa) astheir primary source of calories, making the optimization of rice agriculture immensely important. One of the primary constraints to rice agriculture is the de-domesticated (feral) form of rice known as ‘weedy rice’ that aggressively competes for space, soil nutrients, and light. Heavy infestation can reduce crop yields by as much as 80%. As a closely-related weedy descendant of cultivated rice, chemical control is difficult in rice fields, and physical weeding is labor intensive, time consuming, and largely ineffective due to early life-stage mimicry of the crop. Weedy …

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Genetic Risk Factors For Neurodevelopmental Disorders: Insights From Hipsc-Cerebral Organoids, Michelle L. Wegscheid Dec 2021

Genetic Risk Factors For Neurodevelopmental Disorders: Insights From Hipsc-Cerebral Organoids, Michelle L. Wegscheid

Arts & Sciences Graduate Student Theses and Dissertations

Neurofibromatosis type 1 (NF1) is a common neurodevelopmental disorder (NDD) characterized by remarkable phenotypic variability, where affected children manifest a spectrum of central nervous system (CNS) abnormalities, including brain tumors, impairments in attention, behavior, learning disabilities, and an increased incidence of autism spectrum disorder (ASD). A significant barrier to the implementation of precision medicine strategies for children with NF1 is a lack of prognostic risk factors to guide clinical management. However, emerging population-based genotype-phenotype association studies have suggested that the germline NF1 gene mutation may represent one clinically actionable risk factor for NF1-associated neurodevelopmental abnormalities. As a critical step in …

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Human Plcg2 Haploinsufficiency Results In A Novel Immunodeficiency, Joshua Brandon Alinger Dec 2021

Human Plcg2 Haploinsufficiency Results In A Novel Immunodeficiency, Joshua Brandon Alinger

Arts & Sciences Graduate Student Theses and Dissertations

NK cells are critical for the recognition and lysis of herpesvirus-infected cells. Patients with NK cell immunodeficiency may suffer from unusually severe and/or recurrent herpesvirus infections; however, the genetic cause is frequently unknown. PLCG2 encodes a signaling protein in NK cell and B cell receptor signaling, in which dominant-negative or gain-of-function mutations may cause cold urticaria, antibody deficiency, or autoinflammation. However, loss-of-function mutations and PLCG2 haploinsufficiency have never been reported in human disease. We examined 2 families with autosomal dominant NK cell immunodeficiency with dual high-dimensional techniques, mass cytometry and whole-exome sequencing, to identify the cause of disease. We identified …

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Slo2.1 Channels: A New Molecular Mechanism To Regulate Uterine Excitability, Juan Jose Ferreira Dec 2021

Slo2.1 Channels: A New Molecular Mechanism To Regulate Uterine Excitability, Juan Jose Ferreira

Arts & Sciences Graduate Student Theses and Dissertations

At the end of pregnancy, the uterus transitions from a non-contractile state to a highly contractile state. Two processes primarily drive this transition. First, from the 28th week of pregnancy until labor, the resting membrane potential of uterine (myometrial) smooth muscle cells (MSMCs) gradually becomes more positive (depolarizes) (Parkington et al. 1999). Second, at the end of pregnancy, MSMCs express more oxytocin receptors and become more sensitive to oxytocin (Kimura et al. 1996). However, the detailed mechanisms by which these processes occur have not been determined. My central hypothesis was that the Na+-activated K+ channel SLO2.1 plays a key role …

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Developments In Proteomics, Trans-Splicing Technology And Endogenous Transcript Manipulation, Justin Alexander Melendez Dec 2021

Developments In Proteomics, Trans-Splicing Technology And Endogenous Transcript Manipulation, Justin Alexander Melendez

Arts & Sciences Graduate Student Theses and Dissertations

Technological innovation drives scientific discovery, unlocks new avenues of research, and allows us to ask questions in ways that were previously unavailable. With each technological advance, our ability to perturb and explore biological systems has grown in ways previously unimagined. The theme of my thesis is the development of new technologies in biology. To this end, I have worked on three technologies that contribute to the areas of protein sequencing, RNA barcoding for trans-splicing and single-cell applications, and a new method for transcriptional knockdown. In my first project, digital analysis of proteins by end sequencing (DAPES), we set out to …

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The Effects Of Molecular Chaperone Modulation On Protein Folding, Prion Formation, And Prion Propagation In Saccharomyces Cerevisiae, Leeran Blythe Dublin Ryan Dec 2021

The Effects Of Molecular Chaperone Modulation On Protein Folding, Prion Formation, And Prion Propagation In Saccharomyces Cerevisiae, Leeran Blythe Dublin Ryan

Arts & Sciences Graduate Student Theses and Dissertations

Proper and efficient protein folding is vital for cell survival. Many factors affect protein folding fidelity and prion formation, including molecular chaperone availability and activity. Research has shown that modulating chaperone availability and function can affect protein misfolding and aggregation, as well as de novo prion formation and propagation. However, the factors involved and underlying mechanisms influencing prion formation and protein folding are largely unknown. The following work aims to elucidate these areas. The Nascent Polypeptide-Associated Complex (NAC) is the first point of chaperone contact for nascent polypeptides. Previous work has shown that disruption of the NAC leads to improved …

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Causal Function And Bias Correlation Of The Orbitofrontal Cortex In Economic Choices, Shi Weikang Dec 2021

Causal Function And Bias Correlation Of The Orbitofrontal Cortex In Economic Choices, Shi Weikang

Arts & Sciences Graduate Student Theses and Dissertations

Economic choices entail two mental processes, value calculation and value comparison (Niehans, 1990). Studies in the last twenty years have shown that neurons in the orbitofrontal cortex (OFC) could support both processes. Namely, in the studies in which monkeys chose between two juice options with various amounts, three functional cell groups had been found in the OFC: offer value cells encode the value of individual juices, chosen juice cells encode the choice in a binary way and chosen value cells encode the value of the chosen juice (Padoa-Schioppa and Assad, 2006). These results suggest a decision circuit within OFC with …

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Role Of Interferon Receptors In Sting-Associated Autoinflammatory Disease In Mice, William Alexander Stinson Dec 2021

Role Of Interferon Receptors In Sting-Associated Autoinflammatory Disease In Mice, William Alexander Stinson

Arts & Sciences Graduate Student Theses and Dissertations

Over the past decade, the cGAS-STING pathway has emerged as a principal component of innate DNA sensing. Its activation of type I interferon (IFN) elicits host immune activation and in the absence of cGAS or STING, mice are rendered critically susceptible to infection by viruses and bacteria. Breakdowns in the molecules that regulate this pathway can promote unrestrained cGAS-STING activation and the development of various forms of autoimmune disease. For instance, gain-of-function mutations in STING cause autoinflammatory lung disease in mice and STING-associated vasculopathy with onset in infancy (SAVI) in humans. However, our understanding of the mechanisms that underlie STING-dependent …

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Regulation Of Metabolic Stress By The Snhg3 Locus, Arthur Curtis Sletten Dec 2021

Regulation Of Metabolic Stress By The Snhg3 Locus, Arthur Curtis Sletten

Arts & Sciences Graduate Student Theses and Dissertations

Dyslipidemia and lipotoxicity are pathologic signatures of the metabolic syndrome and type 2 diabetes. Excess lipid causes cell dysfunction and induces cell death through pleiotropic mechanisms that link to oxidative stress. However, pathways that regulate the response to metabolic stress are not fully understood. To identify novel genes involved in metabolic stress, our group performed an unbiased forward genetic screen for lipotoxicity resistance. My studies focused on characterizing one of the mutant cell lines isolated from this screen, in which promoter trap mutagenesis disrupted one allele of the small nucleolar RNA hosting gene 3 (Snhg3). I demonstrate that diminished expression …

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Cancer Epigenome Reprogramming, Jennifer Ann Karlow Dec 2021

Cancer Epigenome Reprogramming, Jennifer Ann Karlow

Arts & Sciences Graduate Student Theses and Dissertations

The identification of recurrent genetic mutations in cancer and their functionalcharacterization has provided a strong foundation for our understanding of tumorigenesis. The more recent observation of recurrent and specific epigenetic changes also present in cancer has widened this view, now establishing cancer as a disease of both genetic and epigenetic misregulation. Enhancers, genomic regions primarily responsible for tissue-specific gene expression, have been shown to be frequent targets of both genetic and epigenetic abnormalities. The observation that DNA methylation within regulatory regions has traditionally correlated with reduced gene expression, coupled with the known role of enhancers in regulating tissuespecific gene expression, …

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Influence Of Focal Activity On Macroscale Brain Dynamics In Health And Disease, Zachary Pollack Rosenthal Dec 2021

Influence Of Focal Activity On Macroscale Brain Dynamics In Health And Disease, Zachary Pollack Rosenthal

Arts & Sciences Graduate Student Theses and Dissertations

Macroscopic recordings of brain activity (e.g. fMRI, EEG) are a sensitive biomarker of the neural networks supporting neurocognitive function. However, it remains largely unclear what mechanisms mediate changes in macroscale networks after focal brain injuries like stroke, seizure, and TBI. Recently, optical neuroimaging in animal models has emerged as a powerful tool to begin addressing these questions. Using widefield imaging of cortical calcium dynamics in mice, this dissertation investigates the mechanisms by which focal disruptions in activity alter brain-wide functional dynamics. In two chapters, I demonstrate 1) that focal sensory stimulation elicits state-dependent, global slow waves propagating from primary somatosensory …

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Dissecting The Molecular Mechanism Of Familial Cardiomyopathies, Sarah Ruth Clippinger Schulte Dec 2021

Dissecting The Molecular Mechanism Of Familial Cardiomyopathies, Sarah Ruth Clippinger Schulte

Arts & Sciences Graduate Student Theses and Dissertations

Familial cardiomyopathies, including hypertrophic (HCM), restrictive (RCM) and dilated cardiomyopathy (DCM), are the leading cause of sudden cardiac death in young people. These diseases, which are characterized by altered cardiac contractility and remodeling of the heart, can lead to heart failure. These diseases are primarily caused by point mutations in sarcomeric proteins that generate or regulate heart contraction, such as troponin T. In the heart, the troponin complex together with tropomyosin lie along the actin filament and regulate myosin’s ability to bind actin and produce force. Here I show how mutations in troponin T affect contractility at the molecular level …

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On Arousal And The Internal Regulation Of Brain Function: Theory And Evidence Across Modalities And Species, Ryan Raut Dec 2021

On Arousal And The Internal Regulation Of Brain Function: Theory And Evidence Across Modalities And Species, Ryan Raut

Arts & Sciences Graduate Student Theses and Dissertations

The brain is an organ. It is subject to the same physiological regulatory processes that engage the rest of the body’s organs, sculpted over hundreds of millions of years to sustain life so effectively. The central message of this thesis is that the holistic functioning of the brain, rather than operating at some level above or independent from these systemic regulatory processes, is deeply related to them. In short, as our limited attention spans might suggest: brain function is internally regulated. I propose that this internal regulation is a primary function of intrinsic brain activity. Chapter 2 provides a theoretical …

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Structural Variants Are A Major Source Of Gene Expression Differences In Humans And Often Affect Multiple Nearby Genes, Alexandra Jane Scott Dec 2021

Structural Variants Are A Major Source Of Gene Expression Differences In Humans And Often Affect Multiple Nearby Genes, Alexandra Jane Scott

Arts & Sciences Graduate Student Theses and Dissertations

Structural variants (SVs), including copy number variants (CNVs), balanced rearrangements, and mobile element insertions (MEIs), are an important source of diversity in the human genome but their functional effects are not well understood. SVs are technically difficult to detect and genotype1, and mapping is dependent on deep whole genome sequencing (WGS) which was, until recently, unaffordable for large cohorts. For these reasons SVs are not included in most genome-wide studies of functional variants, despite the fact that SVs are known causal agents in multiple clinical disorders2-16. However, recent advancements in high-throughput sequencing technologies that allow for widespread use of WGS, …

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Deep Multi-Omics Investigations Elucidate Novel Oncogenesis Paradigms, Therapeutic Targets, And Mechanisms Of Treatment Resistance In Cancer, Daniel Cui Zhou Dec 2021

Deep Multi-Omics Investigations Elucidate Novel Oncogenesis Paradigms, Therapeutic Targets, And Mechanisms Of Treatment Resistance In Cancer, Daniel Cui Zhou

Arts & Sciences Graduate Student Theses and Dissertations

No abstract provided.

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Structural Stability Of Short Latency Alfalfa-Based Pastures In The Humid Pampa, Argentina, R. O. Refi, B. Martín Dec 2021

Structural Stability Of Short Latency Alfalfa-Based Pastures In The Humid Pampa, Argentina, R. O. Refi, B. Martín

IGC Proceedings (1977-2023)

The aim of this study was to search the effects of grazing intensity and nitrogen fertilization on the structural stability of a mixed pasture composed by alfalfa (latency group 9), brome grass, tall fescue and white clover, sown in April 1997. Rotational grazing was carried out with dairy cattle with high intensity (HI: up to 3-4 cm) or moderate intensity (LI: up to 8-10 cm), whenever the alfalfa showed basal regrowth. According to a split-plot design, grazing intensities were subdivided to assign three levels of nitrogen fertilization: N0, without fertilization; N1 with 50 kg N/ha per year; and N2 with …

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Late Winter Dietary Overlap Among Greater Rheas And Domestic Herbivores On The Argentinean Flooding Pampa, G. Vacarezza, M. S. Cid, F. Milano Dec 2021

Late Winter Dietary Overlap Among Greater Rheas And Domestic Herbivores On The Argentinean Flooding Pampa, G. Vacarezza, M. S. Cid, F. Milano

IGC Proceedings (1977-2023)

This study evaluates the dietary overlap among greater rheas (Rhea americana L.) sheep and cattle in the Flooding Pampa, Buenos Aires, Argentina during late winter, when is observed the lowest forage availability. The work was carried out with the following forage classes (FC): warm and cool season grasses and forbs (legumes and no-legumes). Diet botanical composition was estimated by microanalysis of faeces. Principal Component Analysis and Kulcyznsky´s index of similarity were used for data analysis. Rhea populations selected diets with higher forb percentages. On the contrary, vegetation structure and their own morpho-physiology conditioned cattle to diets almost exclusively gramineous. …

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Beef Calf Production Using Year-Round Grazing On Tropical Grass Pasture In Summer Combined With Cool Temperate Grass Pasture In Winter, Y. Nakanishi, K. Shindou, S. Watanabe, K. Yamamoto, K. Hagino Dec 2021

Beef Calf Production Using Year-Round Grazing On Tropical Grass Pasture In Summer Combined With Cool Temperate Grass Pasture In Winter, Y. Nakanishi, K. Shindou, S. Watanabe, K. Yamamoto, K. Hagino

IGC Proceedings (1977-2023)

Bahiagrass (Paspalum notatum Fluegge) showed a poor quality with low crude protein in summer but blood biochemical levels of grazing beef cows were similar to drylot feeding cows except of concentration of urea nitrogen. Italian ryegrass (Lolium multiflorum Lam) showed very good quality with high crude protein (about 20 percentage) in winter. And so, suckler cows gained live weight in winter and suckling calves were showed very high growth rate (daily gain was about 1.0 kg). Total live weight gains of grazing cows were higher than those of drylot feeding cows. Postpartum reproductive performances of grazing cows were …

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Winter Grazing And Feeding Systems In Western Canada, Duane H. Mccartney, V. Baron, J. Basarab, E. Okine, G. Lastiwka, A. Depalme, D. Young Dec 2021

Winter Grazing And Feeding Systems In Western Canada, Duane H. Mccartney, V. Baron, J. Basarab, E. Okine, G. Lastiwka, A. Depalme, D. Young

IGC Proceedings (1977-2023)

Beef cow calf producers in Western Canada are looking for methods of extending the grazing season into the winter in order to lower winter feed costs. Dry beef cows were early or late weaned and grazed during the early months of winter on late seeded barley swaths (Hardlum vulgare L.) and compared to cows fed barley silage and free choice barley straw in a feeding yard. The swath grazed barley was late seeded in June and swathed in the silage stage in mid September. Cows successfully swath grazed for three winters from mid November until mid to late February …

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Forage Management For Extending The Grazing Season, David W. Koch, C. M. Lux, J. W. Flake Dec 2021

Forage Management For Extending The Grazing Season, David W. Koch, C. M. Lux, J. W. Flake

IGC Proceedings (1977-2023)

Studies were conducted to determine ways to extend grazing season with forages adequate to meet the needs of grazing animals and to reduce the cost of livestock production. Perennial grasses were swathed, windrowed and left in the field until they were winter-grazed with bred heifers. Corn (Zea mays L.) was stockpiled for winter grazing with lambs and ewes. Windrowed grasses increased in acid detergent fiber (ADF) and neutral detergent fiber (NDF) compared with stored hay, but crude protein content, heifer weight gain and body condition change was similar for both forages. An economic value of $218 ha-1 from …

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Acknowledgment Of Reviewers Dec 2021

Acknowledgment Of Reviewers

Journal of Food and Drug Analysis

No abstract provided.

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Molecular Cartography Of Residue Pesticides On Grape Surface In 3d By Ambient Ionization Tandem Mass Spectrometry, Hung Su, Tiao-Lai Huang, Cheng-Ting Chi, Yi-Tzu Cho, Chi-Wei Lee, Jingyueh Jeng, Jentaie Shiea Dec 2021

Molecular Cartography Of Residue Pesticides On Grape Surface In 3d By Ambient Ionization Tandem Mass Spectrometry, Hung Su, Tiao-Lai Huang, Cheng-Ting Chi, Yi-Tzu Cho, Chi-Wei Lee, Jingyueh Jeng, Jentaie Shiea

Journal of Food and Drug Analysis

Thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was used to characterize the residual pesticides that were collected from the surface of a grape with metallic sampling probes. Fungicides, insecticides, and miticides were detected, where results were validated by simple solvent extraction followed by gas chromatography tandem mass spectrometry and liquid chromatography tandem mass spectrometry analyses. To explore the distribution of pesticide residues on grape surfaces, 149 locations of a grape surface were collected and followed by TD-ESI/MS/MS analysis. The molecular cartography was then generated from analysis of residual pesticides on the grape surface in 3D.

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Variations In Bovine Milk Oligosaccharides After Calving Using P-Aminobenzoic Ethyl Ester Closed-Ring Labeling And Negative Ion Electrospray Lc/Ms/Ms, Han-Ju Chien, Yi-Chun Ma, Yi-Feng Zheng, Cheng-Yu Kuo, Wei-Chen Wang, Guor-Rong Her, Chien-Chen Lai Dec 2021

Variations In Bovine Milk Oligosaccharides After Calving Using P-Aminobenzoic Ethyl Ester Closed-Ring Labeling And Negative Ion Electrospray Lc/Ms/Ms, Han-Ju Chien, Yi-Chun Ma, Yi-Feng Zheng, Cheng-Yu Kuo, Wei-Chen Wang, Guor-Rong Her, Chien-Chen Lai

Journal of Food and Drug Analysis

A strategy was proposed to analyze bovine milk oligosaccharides using p-aminobenzoic ethyl ester (ABEE) closed-ring labeling and C18 capillary liquid chromatography negative ion electrospray tandem mass spectrometry. Linkage specific fragment ions were used to identify oligosaccharide isomers. By constructing the mass chromatograms using linkage specific fragment ions, isomers were differentiated based on m/z values as well as temporal separation provided by liquid chromatography. In addition to disialyllactose and the single isomer lacto-N-neohexaose, four pairs of linkage isomers including 3’/6’-sialyllactose (3’/6’-SL), 3’/6’-sialyllactosamine (3’/6’-SLN), 3’/6’-sialylgalactosyl-lactose (3’/6’-SGL), and lacto-N-tetraose/lacto-N-neotetraose (LNT/LNnT) in bovine milk were investigated. Variations of bovine milk oligosaccharides in a …

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Analysis Of Heterocyclic Aromatic Amines Using Selective Extraction By Magnetic Molecularly Imprinted Polymers Coupled With Liquid Chromatography – Mass Spectrometry, Shih-Ci Jhu, Jing-Ying Wang, Hsueh-Ting Wang, Sung-Fang Chen Dec 2021

Analysis Of Heterocyclic Aromatic Amines Using Selective Extraction By Magnetic Molecularly Imprinted Polymers Coupled With Liquid Chromatography – Mass Spectrometry, Shih-Ci Jhu, Jing-Ying Wang, Hsueh-Ting Wang, Sung-Fang Chen

Journal of Food and Drug Analysis

Heterocyclic aromatic amines (HCAs) are highly carcinogenic and mutagenic chemicals. This study reports on the development of magnetic molecularly imprinted polymers (MMIPs) for the purification and quantification of HCAs. A novel magnetic molecularly imprinted polymer was successfully prepared using a surface molecular imprinting method using functionalized Fe particles as the magnetic cores. 2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) was used as a molecular template; methacrylic acid (MAA), ethylene glycol dimethyl acrylate (EGDMA), 2, 2′-Azobis (2-methylpropionitrile) were used as the functional monomer, crosslinker, and initiator, respectively. The use of the template/functional monomer/crosslinking agent at a ratio of 1:4:20 resulted in a product with better adsorption …

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The Impact Of The Rare Disease And Orphan Drug Act In Taiwan, Ning-Chun Hsiang, Weng-Foung Huang, Churn-Shiouh Gau, Teng-Wen Tsai, Lin-Chau Chang Dec 2021

The Impact Of The Rare Disease And Orphan Drug Act In Taiwan, Ning-Chun Hsiang, Weng-Foung Huang, Churn-Shiouh Gau, Teng-Wen Tsai, Lin-Chau Chang

Journal of Food and Drug Analysis

The Rare Disease and Orphan Drug Act (the Act) was enacted in 2000 in Taiwan for the facilitation of the research, development, and accessibility of orphan drugs and special nutritional foods; for the prevention and early diagnosis of rare diseases; and for providing intensive care for patients with rare diseases. The aim was to investigate the impact of the Act on the availability and use of orphan drugs in Taiwan in the hope of identifying the remaining challenges and possible solutions to assist future policy making, which may be applicable in other countries as well. The information and statistics for …

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Determination Of Quetiapine And Its Metabolites In Plasma By Field-Enhanced Sample Stacking, Tai-Ling Liu, Li-Shan Fang, Jing-Ru Liou, Jia-Shan Dai, Yen-Ling Chen Dec 2021

Determination Of Quetiapine And Its Metabolites In Plasma By Field-Enhanced Sample Stacking, Tai-Ling Liu, Li-Shan Fang, Jing-Ru Liou, Jia-Shan Dai, Yen-Ling Chen

Journal of Food and Drug Analysis

Quetiapine is an atypical antipsychotic drug that can be used to treat mental disorders, including schizophrenia, bipolar disorder and Alzheimer's disease. Quetiapine is mainly converted into the active metabolites of norquetiapine and 7-hydroxyquetiapine by the liver enzymes CYP3A4 and CYP2D6. In this study, liquid-liquid extraction (LLE) was used as a sample pretreatment method to eliminate interferences in plasma. tert-Butyl methyl ether was chosen as the extraction solvent. Field-enhanced sample injection (FESS), an online preconcentration technique, was used to analyze quetiapine and its metabolites norquetiapine and 7-hydroxyquetiapine in plasma. The optimal separation condition was 120 mM phosphate (pH 4.0) containing …

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Quantitative Analysis Of Fragrance Allergens In Various Matrixes Of Cosmetics By Liquid-Liquid Extraction And Gc-Ms, Chia-Hui Lu, Ming-Chih Fang, Yu-Zi Chen, Shou-Chieh Huang, Der-Yuan Wang Dec 2021

Quantitative Analysis Of Fragrance Allergens In Various Matrixes Of Cosmetics By Liquid-Liquid Extraction And Gc-Ms, Chia-Hui Lu, Ming-Chih Fang, Yu-Zi Chen, Shou-Chieh Huang, Der-Yuan Wang

Journal of Food and Drug Analysis

Fragrances are the most common chemicals in cosmetics to which people expose every day. However, the unwanted allergic reactions such as contact dermatitis caused by direct contact with fragrances may happen. In Directive 2003/15/EC of the EU, cosmetic product containing one or more of 26 fragrance allergens must be declared on the package label. In addition, commission regulation (EU) 2017/1410 amending Annexes II and III of cosmetic regulation 1223/2009 restricted fragrance chemical of methyl eugenol, and prohibited Lyral, atranol, chloroatranol to be used in cosmetic. In this study, an efficient and sensitive GC-MS method for 3 banned fragrances, 26 fragrance …

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Diagnostic Ions Guided 2d-Locating Strategy For Characterization Of Chemical Analogues In Complex Herbal Medicines Using Liquid Chromatography-Ion Mobility-Mass Spectrometry, Meng-Ning Li, Zi-Xuan Zhang, Hui-Ying Wang, Wen Gao, Ping Li, Hua Yang Dec 2021

Diagnostic Ions Guided 2d-Locating Strategy For Characterization Of Chemical Analogues In Complex Herbal Medicines Using Liquid Chromatography-Ion Mobility-Mass Spectrometry, Meng-Ning Li, Zi-Xuan Zhang, Hui-Ying Wang, Wen Gao, Ping Li, Hua Yang

Journal of Food and Drug Analysis

Rapid characterization of chemical analogues in potentially toxic complex matrix was essential for prevention of accidental poisoning. On the basis of the fragment ions possessed not only the same retention times (RT) but the same drift times (DT) on liquid chromatography-ion mobility-mass spectrometry (LC-IM-MS), an alternating frames (AF)-data independent acquisition (DIA) was utilized for simultaneous detection and rapid match of precursor/product ions with a fast switch of low/high collision energy (CE). A diagnostic ions guided 2D-locating strategy was developed for identification of chemical analogues in potentially toxic herbal medicines using LC-IM-MS. Firstly, the 2D-locations (RT, DT) of diagnostic ions were …

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Pharmacokinetics Of Panduratin A Following Oral Administration Of A Boesenbergia Pandurata Extract To Rats, Jihyun Won, Keumhan Noh, Jae-Kwan Hwang, Beom Soo Shin, Wonku Kang Dec 2021

Pharmacokinetics Of Panduratin A Following Oral Administration Of A Boesenbergia Pandurata Extract To Rats, Jihyun Won, Keumhan Noh, Jae-Kwan Hwang, Beom Soo Shin, Wonku Kang

Journal of Food and Drug Analysis

Boesenbergia pandurata and its major active ingredient, panduratin A (PAN), exhibit antibacterial, anti-oxidant, anti-inflammatory, and anti-obesity effects. We explored the time course of the plasma and tissue (in the major organs, gums and skin) concentrations of PAN after oral administration of a B. pandurata extract to rats. Model-dependent analysis was used to quantify the skin distribution of PAN after systemic exposure. The PAN level peaked at 1.12 ± 0.22 μg/mL after 3 h, and then biexponentially decayed with a terminal half-life of 9 h. The mean clearance (Cl/F) was 2.33 ± 0.68 L/h/kg. The PAN levels in organs were in …

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Identification Of Oxytocin Receptor Activating Chemical Components From Traditional Japanese Medicines, Yuko Maejima, Shoichiro Horita, Shoko Yokota, Tomoyuki Ono, Peter Proks, Hiromi Yoshida-Komiya, Yoichi Ueta, Katsuhiko Nishimori, Shingen Misaka, Kenju Shimomura Dec 2021

Identification Of Oxytocin Receptor Activating Chemical Components From Traditional Japanese Medicines, Yuko Maejima, Shoichiro Horita, Shoko Yokota, Tomoyuki Ono, Peter Proks, Hiromi Yoshida-Komiya, Yoichi Ueta, Katsuhiko Nishimori, Shingen Misaka, Kenju Shimomura

Journal of Food and Drug Analysis

Oxytocin (Oxt) is known to regulate social communication, stress and body weight. The activation of Oxt receptors (OTR) has clinical potential to abate stress disorders and metabolic syndrome. Kamikihito (KKT) is a traditional Japanese medicine used to treat psychological stress-related disorders. We investigated the effects of KKT, its ingredients and chemical components on Oxt neurons and OTR. C-Fos expression was examined after oral and peripheral administration of KKT in rats. Electrophysiological change of Oxt neurons and Oxt release upon application of KKT were measured in rat brain slice. The direct effect of KKT, its ingredients and its chemical components were …

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