Life Sciences Commons^™

Agricultural Support For The Old Colony Mennonites Of Belize: A Collaborative Effort Between North American Old Order Mennonites And A Cornell Extension Educator, Judson Reid

Journal of Amish and Plain Anabaptist Studies

The Old Colony Mennonites of Belize practice a conservative form of Anabaptism where technology, dress and lifestyle choices are informed by church standards. Members live in a spatially contiguous Colony, which creates land pressure over time as population increases. The development of new Colonies is necessary for the Mennonites to participate in agricultural livelihoods. The Indian Creek Colony of Belize has experienced agricultural and economic hardships since its inception in the 1989 due to land costs, crop failures and debt structure. Old Order Mennonites of North America developed an outreach effort to assist with on-farm production of vegetable crops to …

Penn State Extension Services And Plain People: An Inside Perspective On A Trust-Based Relationship, Phillip Martin

Journal of Amish and Plain Anabaptist Studies

This perspective on Penn State Extension Services and plain people is based on my personal experiences as a plain person, in which I interacted with Extension Services first as a farmer, and then (while working on a doctorate) as a part of the Extension system. Penn State Extension started over a century ago and was deliberate in reaching out to plain (conservative Anabaptist) farmers since the beginning, which led to a history of trust-based cooperation. For all these successes there remain challenges to effective cooperation with certain plain individuals and subgroups. I suggest these challenges are broadly similar to those …

Hotline Number To Reach And Offer Agricultural Information To Plain Anabaptists During The Covid-19 Workplace Closures, Emily Shoop, Carly Becker, Nathan Glenn Briggs, Danielle Smarsh

Journal of Amish and Plain Anabaptist Studies

The Penn State Extension Animal Systems Team developed a toll-free hotline phone number to convey short educational messages to maintain contact and outreach to Pennsylvania’s animal producing Amish, Mennonite, and other plain Anabaptist populations. During the COVID-19 pandemic, Penn State Extension’s programming went largely online via webinars, online courses, and emails. This change in programming excluded a large sector of Pennsylvania farmers who do not use or have access to the internet and, as such, were liable to miss important best management practice reviews and timely updates in animal agriculture. The Animal Systems Hotline offered callers the choice to listen …

Providing Agricultural Information To Amish And Mennonite Farmers: Creative Adaptations To Wisconsin Extension Programs And Communications, Richard Halopka

Journal of Amish and Plain Anabaptist Studies

This service provider report taps into the experience of a longtime Extension Educator in Wisconsin. Pulling from his work in Clark County, WI, where Amish and Old Order Mennonites have been particularly important to the local agricultural economy, he provides examples of how Extension can modify programming to meet the needs of plain producers. Topics of interest to Amish and Mennonite farmers include nutrient management, forage quality, and farm safety. Areas of program modification include approaches to communication, training, and support in ways that are culturally sensitive to church restrictions. In-person training and informational voicemail recordings have been particularly useful …

Effective Communication And Programming When Working With Amish Farmers: Reflections From A Wisconsin Agriculture Educator, Vance Haugen

Journal of Amish and Plain Anabaptist Studies

The author, an agricultural educator who worked with the Amish in Wisconsin for over 30 years, discusses his outreach efforts, which have been focused on managed grazing, a method well suited to Amish producers. Managed grazing offers agronomic, economic, and ecological benefits. A key educational tool for communicating managed grazing practices is the pasture walk, and the author relates lessons learned from these events. The communal nature and focus on farmer-to-farmer information exchange is well received by Amish producers. It is helpful if pasture walk scheduling is done well in advance and is focused on farmer-based problems with farmer-based solutions …

Ohio’S Amish And Mennonite Produce Growers: Farm Trends, Information Needs, And Agricultural Communication Preferences, Brad Bergefurd

Journal of Amish and Plain Anabaptist Studies

This study (1) describes the characteristics of Ohio Amish and Mennonite produce farmers and their operations; (2) determines awareness of and participation in OSU Extension programs by Amish and Mennonite produce farmers; (3) determines the methods used by Amish and Mennonite produce farmers to acquire farming information; and (4) identifies subject matter needs of Amish and Mennonite produce farmers for future Extension programming. In order to provide effective future Extension programming efforts for this particular clientele, a mail survey was used to collect data from 345 Ohio Amish and Mennonite farmers who participate at Ohio produce auctions. They were asked …

The Success Of Plain People’S Produce Auctions And Partnership With Missouri Extension, James Quinn, John Kruse, Londa Nwadike, James C. Piñero, David Trinklein

Journal of Amish and Plain Anabaptist Studies

The number of produce auctions led by plain Anabaptist producers has surged since the mid-1990s. Typically grower-owned, these businesses are centralized facilities providing a wholesale market to area growers for a diverse group of buyers. For growers seeking an adequate living from growing produce, these facilities help reduce marketing time and product transportation, and provide frequent sales events that guarantee payment. As such, they have developed into a solid and long-standing market for local produce. However, plain growers have experienced challenges trying new crops or scaling up production. This article details how Missouri Extension assisted plain farmers with their challenges …

Farm Food Safety Training For Amish Produce Growers Covered Under The Food Safety Modernization Act (Fsma), Luke Laborde, Jeffrey Stoltzfus, Kaila Thorn

Journal of Amish and Plain Anabaptist Studies

Commercial production of fruits and vegetables on Amish farms provides significant amounts of fresh produce that are regionally distributed through wholesale markets. In response to several multi-state foodborne disease outbreaks linked to contamination of fresh produce, the U.S. Food and Drug Administration (FDA) mandated farm food safety standards that most commercial produce growers must implement. Although there have been no foodborne disease outbreaks attributed to fresh produce grown on Amish farms, this regulation poses regulatory challenges for those who sell produce at wholesale produce auctions, cooperatives, and distribution warehouses. This article describes recent farm food safety standards issued by the …

Guest Editor’S Introduction: Outreach And Applied Agriculture Research With Plain Anabaptists: Lessons Learned And Future Directions, Caroline Brock

Journal of Amish and Plain Anabaptist Studies

This issue of the Journal of Amish and Plain Anabaptist Studies (JAPAS) contributes to a dearth of literature on ways to understand how plain Anabaptist culture influences agri-environmental beliefs and practices and how it informs outreach and research. Only a few studies currently focus on outreach with plain Anabaptist growers (e.g., Brock, Ulrich-Schad, and Prokopy 2018; Bergefurd 2011; Jepsen and Mann 2015; Stoltzfus 2019; Hoorman and Spencer 2001/2002). This issue of JAPAS is the first comprehensive publication to include research articles and service provider reports focusing on plain people and agriculture. This issue addresses the history of Anabaptist …

Assessment Of Virus-Induced Myocarditis In Human Heart Tissue Samples, Gabriel Galeotos

Honors Theses

The heart is a major organ whose function is to transport nutrients and waste throughout the body. This organ can become infected by pathogens, such as viruses, bacteria, or parasites. Infection of the middle heart layer, or myocardium, is often caused by a viral agent. This disease has three stages: viral infiltration, adaptive immune system activation, and finally either viral clearance or cardiac cell remodeling. During this process the immune system will begin to secrete cytokines, which are signaling molecules that alert other members of the immune pathways, and also participate in cardiac remodeling. Evaluating the correlation between the cytokine …

Genetic Contributors Of Incident Stroke In 10,700 African Americans With Hypertension: A Meta-Analysis From The Genetics Of Hypertension Associated Treatments And Reasons For Geographic And Racial Differences In Stroke Studies, Nicole D. Armstrong, Vinodh Srinivasasainagendra, Amit Patki, Rikki M. Tanner, Bertha A. Hidalgo, Hemant K. Tiwari, Nita A. Limdi, Ethan M. Lange, Leslie A. Lange, Donna K. Arnett, Marguerite R. Irvin

Epidemiology and Environmental Health Faculty Publications

Background: African Americans (AAs) suffer a higher stroke burden due to hypertension. Identifying genetic contributors to stroke among AAs with hypertension is critical to understanding the genetic basis of the disease, as well as detecting at-risk individuals.

Methods: In a population comprising over 10,700 AAs treated for hypertension from the Genetics of Hypertension Associated Treatments (GenHAT) and Reasons for Geographic and Racial Differences in Stroke (REGARDS) studies, we performed an inverse variance-weighted meta-analysis of incident stroke. Additionally, we tested the predictive accuracy of a polygenic risk score (PRS) derived from a European ancestral population in both GenHAT and REGARDS AAs …

Nondestructive Detection Of Codling Moth Infestation In Apples Using Pixel-Based Nir Hyperspectral Imaging With Machine Learning And Feature Selection, Nader Ekramirad, Alfadhl Y. Khaled, Lauren E. Doyle, Julia R. Loeb, Kevin D. Donohue, Raul T. Villanueva, Akinbode A. Adedeji

Electrical and Computer Engineering Faculty Publications

Codling moth (CM) (Cydia pomonella L.), a devastating pest, creates a serious issue for apple production and marketing in apple-producing countries. Therefore, effective nondestructive early detection of external and internal defects in CM-infested apples could remarkably prevent postharvest losses and improve the quality of the final product. In this study, near-infrared (NIR) hyperspectral reflectance imaging in the wavelength range of 900–1700 nm was applied to detect CM infestation at the pixel level for three organic apple cultivars, namely Gala, Fuji and Granny Smith. An effective region of interest (ROI) acquisition procedure along with different machine learning and data processing …

Studying The Behavioral Role Of The Kcnn2 Gene In Zebrafish Embryos, Gina Dragonette

Honors Theses

Introduction: Mutations in the KCNN2 gene have been linked to phenotypes of movement disorders and autism. The goal of this experiment was to use the CIUSPR-Cas9 system to create a deletion within the KCNN2 gene and to observe the effect on the expression of movement in zebra fish embryos.

Methods: Linearized Cas9 DNA and guide DNA underwent in vitm transcription (IVT) reactions to produce RNA to be injected into zebrafish embryos at the one cell stage. KCNN guide RNAs were produced for the KCNN2 and KCNN3 genes (to prevent KCNN3 protein function from compensating for loss ofKCNN2's protein function), and …

Late-Life Exercise Mitigates Skeletal Muscle Epigenetic Aging, Kevin A. Murach, Andrea L. Dimet-Wiley, Yuan Wen, Camille R. Brightwell, Christine M. Latham, Cory M. Dungan, Christopher S. Fry, Stanley J. Watowich

Center for Muscle Biology Faculty Publications

There are functional benefits to exercise in muscle, even when performed late in life, but the contributions of epigenetic factors to late-life exercise adaptation are poorly defined. Using reduced representation bisulfite sequencing (RRBS), ribosomal DNA (rDNA) and mitochondrial-specific examination of methylation, targeted high-resolution methylation analysis, and DNAge™ epigenetic aging clock analysis with a translatable model of voluntary murine endurance/resistance exercise training (progressive weighted wheel running, PoWeR), we provide evidence that exercise may mitigate epigenetic aging in skeletal muscle. Late-life PoWeR from 22–24 months of age modestly but significantly attenuates an age-associated shift toward promoter hypermethylation. The epigenetic age of muscle …

Adipose-Specific Pparα Knockout Mice Have Increased Lipogenesis By Pask–Srebp1 Signaling And A Polarity Shift To Inflammatory Macrophages In White Adipose Tissue, Terry D. Hinds, Jr., Zachary A. Kipp, Mei Xu, Frederique B. Yiannikouris, Andrew J. Morris, Donald F. Stec, Walter Wahli, David E. Stec

Pharmacology and Nutritional Sciences Faculty Publications

The nuclear receptor PPARα is associated with reducing adiposity, especially in the liver, where it transactivates genes for β-oxidation. Contrarily, the function of PPARα in extrahepatic tissues is less known. Therefore, we established the first adipose-specific PPARα knockout (Ppara^FatKO) mice to determine the signaling position of PPARα in adipose tissue expansion that occurs during the development of obesity. To assess the function of PPARα in adiposity, female and male mice were placed on a high-fat diet (HFD) or normal chow for 30 weeks. Only the male Ppara^FatKO animals had significantly more adiposity in the inguinal white …

The Effects Of Boric Acid Sugar Bait On Wolbachia Trans-Infected Male Aedes Albopictus (Zap Males®) In Laboratory Conditions, Vindhya S. Aryaprema, Whitney A. Qualls, Karen L. Dobson, Stephen L. Dobson, Rui-De Xue

Entomology Faculty Publications

The field release of Wolbachia trans-infected male mosquitoes, as well as the use of toxic sugar baits, is a novel and promising candidate technique for integrated mosquito management programs. However, the methods of action of the two techniques may not be complementary, because the Wolbachia method releases mosquitoes into the environment expecting a wild population reduction in subsequent generations while the toxic baits are intended to reduce the wild population by killing mosquitoes. This laboratory study was conducted to evaluate the effectiveness of boric acid toxic sugar baits on Wolbachia trans-infected male Aedes albopictus, relative to wild-type Ae. albopictus …

Linking Migration To Community Resilience In The Receiving Basin Of A Large-Scale Water Transfer Project, Anna Erwin, Zhao Ma, Ruxandra Popovici, Emma Patricia Salas O’Brien, Laura Zanotti, Chelsea A. Silva, Eliseo Zeballos Zeballos, Jonathan Bauchet, Nelly Ramírez Calderón, Glenn Roberto Arce Larrea

Department of Agricultural Economics Faculty Publications

Large-scale water transfer projects (LWTPs) transfer water to urban and agricultural areas. The Majes-Siguas canal, established in 1983, is an LWTP that created a thriving agricultural area through irrigating the Majes district in the Atacama Desert of Peru. Like other LWTP receiving basins, the project has attracted an influx of migrants who work on the farms. At the same time, the Majes LWTP is the district’s only source of water and has an aging infrastructure which presents significant risks. While many studies critically analyze the consequences of LWTPs in water supply basins, few evaluate the resilience of communities living in …

Induction Of Il19 Expression Through Jnk And Cgas-Sting Modulates Dna Damage–Induced Cytokine Production, Sara H. Small, E. Jessica Tang, Ryan L. Ragland, Yaroslava Ruzankina, David W. Schoppy, Rahul S. Mandal, M. Rebecca Glineburg, Zgjim Ustelenca, Daniel J. Powell, Fiona Simpkins, F. Bradley Johnson, Eric J. Brown

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Cytokine production is a critical component of cell-extrinsic responses to DNA damage and cellular senescence. Here, we demonstrated that expression of the gene encoding interleukin-19 (IL-19) was enhanced by DNA damage through pathways mediated by c-Jun amino-terminal kinase (JNK) and cGAS-STING and that IL19 expression was required for the subsequent production of the cytokines IL-1, IL-6, and IL-8. IL19 expression was stimulated by diverse cellular stresses, including inhibition of the DNA replication checkpoint kinase ATR (ataxia telangiectasia and Rad3-related protein), oncogene expression, replicative exhaustion, oxidative stress, and DNA double-strand breaks. Unlike the production of IL-6 and IL-8, IL19 expression was …

Sap Beetles, Nick Volesky, Zachery R. Schrumm, Kalen Taylor

All Current Publications

Sap beetles are typically considered a secondary pest of corn and overripe fruits and vegetables. They are broadly identified by their small and ovular bodies and club-shaped antennae. Adult sap beetles often feed on corn silk, pollen, and tassels. Larvae feed on kernels inside the husk. This fact sheet reviews how to identify sap beetles and their hosts and damage. It also provides ideas on how to monitor for sap beetles and outlines the options for managing them.

Sheep Performance In Italian Ryegrass Swards At Contrasting Sward Heights, Paulo C. De F. Carvalho, L. S. Pontes, E. O. Silveira, C. H. E. C. Poli, Carlos Nabinger, O. A. Pereira Neto, G. E. Maraschin

IGC Proceedings (1977-2023)

The objective of this study was to assess the effect of Italian ryegrass (Lolium multiflorum Lam.) sward height on lamb performance. The experiment was carried out at the Research Station of UFRGS, Eldorado do Sul, Brazil. Yearling no-castrated male lambs were assessed in terms of individual animal performance and gain per hectare. The experiment was carried out in a randomised block design with three replications of four treatments: 5, 10, 15 and 20 cm of sward surface height (SSH), which were maintained using continuous variable stocking, and monitored by a sward stick. Samples of 0.25 m² were cut …

Animal Performance In Pastures Of Panicum Maximum Cv. Ipr 86 Fertilized With Nitrogen, S. M. B. Lugão, L. R. De A. Rodrigues, E. B. Malheiros, J. J. Dos S. Abrahão, A. De Morais

IGC Proceedings (1977-2023)

The experiment was carried out at the Experimental Station of the IAPAR in Paranavaí– PR, Brazil, from October 1998 to May 1999 to evaluate the response of crossbred steers grazing pastures of Panicum maximum cv. IPR 86 fertilized with four doses of N (0; 150; 300 and 450 kg.ha^-1.year). The grass was grazed according to a rotational grazing system with grazing cycles (GCs) of 40 days (grazing period of 5 days and rest period of 35 days). It was used a randomized complete block design, with split-plots, and two replications per treatment, being the N-doses studied in the …

Dairy Cow Performance On Pasture-Based Feeding Systems And In Confinement, Renato S. Fontaneli, Lynn E. Sollenberger, C. R. Staples

IGC Proceedings (1977-2023)

Interest in grazing systems is growing among farmers in the USA as a means of reducing feed costs for lactating dairy cows. An experiment was conducted near Gainesville, FL to compare milk production and composition and milk income minus feed costs from two pasture-based systems with those of a conventional confinement housing system over a 276-d period. System 1 was based on a mixture of rye (Secale cereale L.), annual ryegrass (Lolium multiflorum Lam.), crimson clover (Trifolium incarnatum L.), and red clover (Trifolium pratense L.) during the winter-spring seasons and pearl millet (Pennisetum glaucum [L.] …

Monitoring Photosynthetic Activity Using In Vivo Chlorophyll A Fluorescence In Microalgae And Cyanobacteria Biofilms In The Nerja Cave (Malaga, Spain), Yolanda Del Rosal, Juan Muñoz-Fernández, Paula S.M. Celis-Plá, Mariona Hernández-Mariné, Félix Álvarez-Gómez, Salvador Merino, Félix L. Figueroa

International Journal of Speleology

The characterization of the most common photosynthetic biofilms in the Nerja Cave by the continuous monitoring of the in vivo chlorophyll a (Chl a) fluorescence and the incorporation of the irradiance as a new environmental variable related to previous studies in the cave, have allowed us to improve our knowledge about the photosynthetic pattern of the biofilms of the cave. Effective quantum yield (ΔF/F_m′) and relative electron transport rate (rETR) were determined during periods of the light, whereas the maximal quantum yield (F_v /F_m) was determined during dark periods. Increases in …

Ecological Risk Assessment For The State-Wide Small Pelagic Scalefish Resource, S. Blazeski, J. Norriss, K. A. Smith, M. Hourston

Fisheries Research Reports

In July 2021, the Department of Primary Industries and Regional Development convened an ecological risk assessment (ERA) of the fisheries that access the State-wide Small Pelagic Scalefish Resource. The ERA considered the potential ecological impacts of the West Coast Purse Seine Fishery, South Coast Purse Seine Fishery, Purse Seine Development Zones and the recreational fishers who catch small pelagic scalefish. The assessment evaluated the impact of each fishing sector/method on all relevant retained and bycatch species, endangered, threatened and protected species, habitats and the broader environment.

Cryptococcus Neoformans Melanization Incorporates Multiple Catecholamines To Produce Polytypic Melanin, Rosanna P. Baker, Christine Chrissian, Ruth E. Stark, Arturo Casadevall

Publications and Research

Melanin is a major virulence factor in pathogenic fungi that enhances the ability of fungal cells to resist immune clearance. Cryptococcus neoformans is an important human pathogenic fungus that synthesizes melanin from exogenous tissue catecholamine precursors during infection, but the type of melanin made in cryptococcal meningoencephalitis is unknown. We analyzed the efficacy of various catecholamines found in brain tissue in supporting melanization using animal brain tissue and synthetic catecholamine mixtures reflecting brain tissue proportions. Solid-state NMR spectra of the melanin pigment produced from such mixtures yielded more melanin than expected if only the preferred constituent dopamine had been incorporated, …

Behavioral Patterns Of Goldfish (Carassius Auratus) Exploring A “Fish Tower”, Sasha Prasad-Shreckengast

Theses and Dissertations

Fish are held in captivity at some of the highest numbers of any taxa, but their curiosity is rarely studied. With this study, we set the groundwork to investigate the presence and nature of curiosity in goldfish (Carassius auratus) via novel free-choice exploration opportunities by creating a “fish tower."

Functional Characterization Of Arabidopsis Synaptotagmin-4 Protein Through Reverse Genetics Strategy, Zahid Jawed

Theses and Dissertations

Calcium ion plays a significant role in plant signal transduction pathways, including regulation of stress-induced gene expression. Sensor proteins such as proteins with calcium-binding C2 domains can perceive changes in cellular calcium level. Functional analysis of such sensor proteins will give us a better understanding of plant stress signaling. Synaptotagmins are a group of proteins containing C2 domains that are capable of binding calcium ions and regulate gene expression in response to external abiotic stress stimuli. Here, we used a reverse genetic approach to analyze the function of synaptotagmin – 4, which is one of the five identified synaptotagmin proteins …

Domestication Reshaped The Genetic Basis Of Inbreeding Depression In A Maize Landrace Compared To Its Wild Relative, Teosinte, Luis Fernando Samayoa, Bode A. Olukolu, Chin Jian Yang, Qiuyue Chen, Markus G. Stetter, Alessandra M. York, Jose De Jesus Sanchez-Gonzalez, Jeffrey C. Glaubitz, Peter J. Bradbury, Maria Cinta Romay, Qi Sun, Jinliang Yang, Jeffrey Ross-Ibarra, Edward S. Buckler, John F. Doebley, James B. Holland

Department of Agronomy and Horticulture: Faculty Publications

Inbreeding depression is the reduction in fitness and vigor resulting from mating of close relatives observed in many plant and animal species. The extent to which the genetic load of mutations contributing to inbreeding depression is due to large-effect mutations versus variants with very small individual effects is unknown and may be affected by population history. We compared the effects of outcrossing and self-fertilization on 18 traits in a landrace population of maize, which underwent a population bottleneck during domestication, and a neighboring population of its wild relative teosinte. Inbreeding depression was greater in maize than teosinte for 15 of …

Environmental Impacts Of Contact Lens Waste, Abigail Wilhelmi

Arts & Sciences Undergraduate Showcase

Contact lenses have been used for decades to correct vision, and over 150 million people use contact lenses today. The materials used to make contact lenses have evolved drastically, but ultimately resulted in a specific plastic, silicone-derived hydrogel, which provides maximal comfort and clear vision, while also maintaining the eye health of the user. Contact lenses have enhanced the lives of many people, but a topic that is often left out of contact lens discussions is that of plastic waste. Single-use plastic and increased plastic pollution have recently been areas of concern and expanded research. Contact lenses are often forgotten …

Deep Learning For Human Microrna Precursor Prediction: A Systematic Literature Review, Martina Ojonah

Master of Science in Information Technology Theses

The discovery of MicroRNA (miRNA) sparked medical breakthroughs, leading to the development of drugs/vaccines and biomarkers for some terminal diseases such as cancer. Due to the relatively short length of microRNAs, research has shown that discovering them on their own is a difficult task; thus, the focus has shifted to predicting precursor miRNAs, which are longer than miRNAs. Computational techniques evolve because of flaws discovered in existing designs, discoveries, and the desire to make the process as seamless as possible. Most researchers in recent studies indicated that the use of few input features and a lack of domain understanding of …