Life Sciences Commons^™

Role Of Jak/Stat Signaling Pathway During Viral Infection In Black Tiger Shrimp Penaeus Monodon, Pasunee Laohawutthichai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Janus Kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway plays an important role for antiviral immunity. To stimulate the pathway, the cytokines, interferons, or other stimulators activate the receptor, which is then dimerized and activate JAKs, causing phosphorylation of the tyrosine residues of the receptor. Consequently, STATs are phosphorylated, dimerized and localized into the nucleus to regulate their target gene transcription. This study aims to better understand the role of JAK/STAT signaling pathway in Penaeus monodon during viral infection. From tissue distribution, PmDOME, PmJAK and PmSTAT transcripts were expressed in all tested tissues and up-regulated about 2-fold upon WSSV …

Macrophage-Cancer Cell Interaction In Three Dimension Liver Cancer Model, Pornlapat Keawvilai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hepatocellular carcinoma (HCC) is the one of the leading causes of cancer-related mortality. Liver cancer progression is significantly influenced by immune cells which infiltrate tumors and their interaction with liver cancer cells in the tumor microenvironment (TME). Circulating monocytes are recruited to tumor site by TME and differentiated into tumor-associated macrophages (TAMs) which are roughly divided into pro-inflammatory macrophages (M1) or pro-tumoral macrophages (M2). In order to study the interaction between macrophages and liver cancer cells. In in vitro, an effective liver cancer model which closely mimicks this crosstalk is necessary. This study aimed to fabricate a three-dimension (3D) liver …

Analysis Of Viral Genes From Whole Genome Sequencing Data Of Thai Patients With Brugada Syndrome, Suwalak Chitcharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Brugada syndrome (BS) is an autosomal dominant inheritance cardiac arrhythmia disorder associated with sudden death in young adults. Thailand has the highest prevalence of BS worldwide, and over 60% of patients with BS still have unclear etiology of the disease. In this study, we developed a metagenomic analysis pipeline for identifying the viral gene from Whole genome sequencing (WGS) data, which is available on Github. Then, WGS data of BS patients (n=100) and control volunteers (n=100) were investigated. Five viruses, including Torque teno virus 19, Torque teno virus 8, Torque teno virus 3, Aeribacillus virus AP45 and Gemykibivirus humas3, were …

Theoretical Simulations Between Musashi Proteins And Target Rna, Nitchakan Darai

Chulalongkorn University Theses and Dissertations (Chula ETD)

The Musashi (MSI) family of RNA-binding proteins, comprising the two homologs Musashi-1 (MSI1) and Musashi-2 (MSI2), typically regulates translation and is involved in cell proliferation and tumorigenesis. However, the focus of this study is MSI1 because it is highly expressed in neural stem cells in their immature condition. MSI1 is highly enriched in neural precursor cells and develops the central nervous system, it is highly expressed in embryonic, fetal, and adult neural stem cells. The finding that MSI also promotes the replication of the Zika virus, a neurotropic Flavivirus, has triggered further investigations of the biochemical principles behind MSI1-RNA interactions. …

Identification Of Aroma Compounds In Thai Colored Rice Varieties, Supawat Jindawatt

Chulalongkorn University Theses and Dissertations (Chula ETD)

Consumers are interested in colored rice because its health benefits and its high fiber and protein content. Normally, the colored rice smell is generally not as strong as the jasmine rice. The first objective of this research was to investigate the proper preheated time and headspace incubation temperature to extract as many volatile compounds as possible from Thai-colored rice using the static headspace GC-MS approach. Secondly, this study aimed to identify the key volatile aroma compounds in Thai native-colored rice varieties. For optimum conditions, Red Hawm Rice (KDML105R-PSL-E-14) was used in this study. The optimal condition was preheated in a …

การพัฒนาชุดตรวจออกซิไดซ์ไลโพโปรตีนชนิดความหนาแน่นสูง ด้วยเทคนิค Paper-Based Immunogold-Silver Enhancement Assay, ปิยวดี ประดิษฐ์นวกุล

Chulalongkorn University Theses and Dissertations (Chula ETD)

ไลโพโปรตีนชนิดความหนาแน่นสูง (High-density lipoprotein; HDL) มีบทบาทสำคัญในการป้องกันการเกิดโรคหัวใจขาดเลือด ต้านการอักเสบของหลอดเลือด และยับยั้งการเกิด LDL-oxidation แต่การทำงานของ HDL จะสูญเสียไปเมื่อ HDL ถูกทำลายด้วยอนุมูลอิสระ กลายเป็น oxidized HDL (oxHDL) การวัดระดับ HDL-cholesterol เป็นการประเมินด้านปริมาณแต่ไม่สามารถบอกการทำงานของ HDL ได้ หากสามารถวัดระดับ oxHDL ควบคู่ไปกับระดับ HDL-cholesterol ทำให้การพยากรณ์การเกิดโรคหัวใจขาดเลือดได้แม่นยำขึ้น แต่การวิเคราะห์ระดับ oxHDL ต้องใช้เครื่องมือจำเพาะและน้ำยามีราคาสูง งานวิจัยนี้มีวัตถุประสงค์เพื่อพัฒนาชุดตรวจวิเคราะห์ oxHDL ด้วยหลักการ Paper-based Immunogold silver-enhanced assay โดยออกแบบฐานกระดาษมีลักษณะเดียวกับ microtiter plate ตรึงแอนติบอดีต่อ 4-Hydroxynonenal (anti-HNE) ซึ่งเป็นผลผลิตจากกรดไขมันที่ถูกออกซิไดซ์ oxHDL ในตัวอย่างจับกับ anti-HNE บนกระดาษ ใช้ anti-APO AI ที่มีความจำเพาะต่อ HDL ที่ติดฉลากกับอนุภาคของทองคำและขยายสัญญาณด้วย silver ion อ่านผลด้วยเครื่องสแกนและวิเคราะห์ความเข้มสีด้วยโปรแกรม Photoshop CS64 ผลการวิเคราะห์ความเป็นเส้นตรงระหว่าง oxHDL กับ ความเข้มสี พบว่า มีความสัมพันธ์เป็นเส้นตรงที่ความเข้มข้นตั้งแต่ 0-300 ng/mL ( r2 = 0.946) ผลการทดสอบความถูกต้องของชุดทดสอบ ใช้ซีรัมจำนวน 32 ราย วิเคราะห์ปริมาณ oxHDL ด้วยวิธีที่พัฒนาขึ้นและวิธี Sandwich ELISA หาความสัมพันธ์ระหว่างปริมาณ oxHDL ที่วิเคราะห์ได้จากทั้งสองวิธีด้วย Pearson correlation coefficient พบว่า ค่า correlation coefficient (r) = 0.527 และมีค่า p-value =0.002 แสดงให้เห็นว่าทั้งสองวิธีมีความสัมพันธ์มีความสัมพันธ์กันในระดับกลางอย่างมีนัยสำคัญทางสถิติ อุปกรณ์ตรวจวิเคราะห์ออกซิไดซ์ไลโพโปรตีนชนิดความหนาแน่นสูงฐานกระดาษเป็นต้นแบบที่สามารถนำไปต่อยอดและพัฒนา เพื่อประยุกต์ใช้ในการตรวจปริมาณ oxHDL …

Defending Human Difference By Raising The Bar, Joe Gough

Animal Sentience

Chapman & Huffman (C&H) offer a theory of why we humans want to believe that we are different: to justify our cruelty to animals. This commentary offers further supporting evidence of this and examines more closely what the claim that humans are ‘different’ amounts to. It also considers some methodological issues in animal psychology closely related to C&H ‘s theory. These problems result from a common strategy for defending hypotheses about human difference.

2022 Weed Science Research Report – Yellow Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2022. It includes work on minor crops and the management of several weeds in North Dakota.

Richard Evans Schultes, James P. Smith Jr

Botanical Studies

No abstract provided.

Poisonous Plants Of Home And Garden, James P. Smith Jr

Botanical Studies

No abstract provided.

Two Hemlocks, James P. Smith Jr

Botanical Studies

No abstract provided.

Food Plants With Toxic Properties, James P. Smith Jr

Botanical Studies

No abstract provided.

An Annotated List Of The Poisonous And Injurious Vascular Plants Of The United States, James P. Smith Jr

Botanical Studies

No abstract provided.

Production Of Vanillate From Lignin Derivatives Using Genetically Modifiedescherichia Coli, Jedsadakorn Ninrat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Vanillic acid is an important compound used in food, beverage, fragrance, and pharmaceutical industries. Vanillic acid can be produced by using either natural plant, native, and engineered microorganisms. However, the industrial scale production of vanillic acid compound still difficult due to low yielding production which can be improved by metabolic engineering of bacterial cells. Hence, the efficient biocatalyst, engineering, and biotechnology process can be a promising strategy to enhance vanillic acid production from lignin derivatives and strengthen the large-scale production for industrial approach. Among several lignin-derived compounds, ferulic acid is a widely used substrate for vanillic acid production. Recently, the …

Isolation And Characterization Of Methyl Viologen-Resistant Synechocystis Sp. Pcc 6803 Mutants, Jidapa Leksingto

Chulalongkorn University Theses and Dissertations (Chula ETD)

Methyl viologen (MV) is a widely used herbicide that competes electrons from photosystem I and induces the formation of active radical species causing cellular oxidative stress in plants and photosynthetic cyanobacteria. However, the exact target molecule of methyl viologen in the cells has yet to be identified. This study aimed to identify the mutation(s) that lead to MV resistance in cyanobacterium Synechocystis sp. PCC 6803. Synechocystis wild type was cultured for 30 days to reach the stationary-growth phase to allow the occurrence of spontaneous mutations in aged cells. Approximately 109 cells were spread on an agar medium containing high MV …

The Covid-19 Pandemic And The Importance Of Financing The Biotechnology Industry In South Africa, Ramazan Uctu, Karen Eksteen

The Journal of Entrepreneurial Finance

Whether it is a government research institution, a public-private partnership, or a private enterprise, the South African biotechnology industry requires funding for the biotechnology industry. Our goal with this research is to look into the role of venture capital (VC) in financing biotech enterprises and demonstrate how VC finance could aid in the development of diagnostic kits and vaccines for Covid-19 or future pandemics in South Africa.

The study aims to provide policymakers with a clear image of the importance of funding the biotechnology sector, notably during the Covid-19 period, as recognized by the industry's key players. We employed a …

In Vivo Activity Of Metal Complexes Containing 1,10-Phenanthroline And 3,6,9-Trioxaundecanedioate Ligands Against Pseudomonas Aeruginosa Infection In Galleria Mellonella Larvae, Megan O'Shaughnessy, Magdalena Piatek, Pauraic Mccarron, Malachy Mccann, Michael Devereux, Kevin Kavanagh, Orla L. Howe

Articles

Drug-resistant Pseudomonas aeruginosa is rapidly developing resulting in a serious global threat. Immunocompromised patients are specifically at risk, especially those with cystic fibrosis (CF). Novel metal complexes incorporating 1,10-phenanthroline (phen) ligands have previously demonstrated antibacterial and anti-biofilm effects against resistant P. aeruginosa from CF patients in vitro. Herein, we present the in vivo efficacy of {[Cu(3,6,9-tdda)(phen)₂]·₃H₂O·EtOH}_n (Cu-tdda-phen), {[Mn(3,6,9-tdda)(phen)₂]·₃H₂O·EtOH}_n (Mn-tdda-phen) and [Ag₂(3,6,9-tdda)(phen)₄]·EtOH (Ag-tdda-phen) (tddaH₂ = 3,6,9-trioxaundecanedioic acid). Individual treatments of these metal-tdda-phen complexes and in combination with the established antibiotic gentamicin were …

Exploring Factors Associated With The Eating Behaviours And Influences On Food Choice In Irish Teens, Aisling Daly

Doctoral

Aim The aim of this research was to understand the eating behaviour traits and food choice motivations of Irish teens, and to investigate the social, psychological, and attitudinal determinants of these eating behaviour traits and influences on food choice.

Methods Mixed methods were used in this research, using quantitative analysis of data from the National Teens’ Food Survey II (NTFSII), a cross-sectional study of teens aged 13-18 years old (N=428). Data predominantly came from the TFEQ-r18 and VARSEEK tools for eating behaviour traits, and the FCQ tool for food choice motivations, as well as socio-demographics, anthropometrics, behaviour/attitudes and dietary intakes. …

Associations Between Eight Earth Observation-Derived Climate Variables And Enteropathogen Infection: An Independent Participant Data Meta-Analysis Of Surveillance Studies With Broad Spectrum Nucleic Acid Diagnostics, Josh M. Colston, Benjamin F. Zaithcik, Hamada S. Badr, Eleanor Burnett, Syed Asad Ali, Ajit Rayamajhi, Syed M. Satter, Daniel Eibach, Ralf Krumkamp, M Imran Nisar, Anita K. M. Zaidi, Zulfiqar Ahmed Bhutta

Department of Paediatrics and Child Health

Diarrheal disease, still a major cause of childhood illness, is caused by numerous, diverse infectious microorganisms, which are differentially sensitive to environmental conditions. Enteropathogen‐specific impacts of climate remain underexplored. Results from 15 studies that diagnosed enteropathogens in 64,788 stool samples from 20,760 children in 19 countries were combined. Infection status for 10 common enteropathogens—adenovirus, astrovirus, norovirus, rotavirus, sapovirus, Campylobacter, ETEC, Shigella, Cryptosporidium and Giardia—was matched by date with hydrometeorological variables from a global Earth observation dataset—precipitation and runoff volume, humidity, soil moisture, solar radiation, air pressure, temperature, and wind speed. Models were fitted for each pathogen, accounting …

Bone, Biomarker, Body Composition, And Performance Responses To 8 Weeks Of Reserve Officers' Training Corps Training, Breanne S. Baker, Samuel R. Buchanan, Christopher D. Black, Michael G. Bemben, Debra A. Bemben

Health & Human Performance Faculty Publications

Context: Military personnel engage in vigorous exercise, often resulting in higher bone mineral density; however, leg bone injuries occur frequently in this population. Predictors of change in tibial bone quality and strength need to be characterized in this high-risk population.

Objectives: To examine the effects of an 8-week military training intervention on total body and site-specific bone density and tibial bone quality, serum biomarkers (parathyroid hormone and sclerostin), body composition, and physical performance and to investigate which outcome variables (biomarkers, body composition, and physical performance) predict estimated tibial bone strength in college-aged Reserve Officers' Training Corps (ROTC) participants.

Design: Prospective …

Interaction Effects Of Age And Body Weight Losses During Moulting On The Performance Ofcommercial Laying Hens, Mian Mubashar Saleem, Jibran Hussain, Usman Elahi, Ehsaan Ullah Khan, Muhammad Usman, Syed Ghulam Mohayudin Hashmi, Sohail Ahmad

Turkish Journal of Veterinary & Animal Sciences

This study evaluated the interaction effects of different age groups and body weight losses during moulting on the productive performance, egg quality, and immune response of commercial laying hens. In total 324 Lohmann Selected Leghorn Lite laying hens of 80 and 108 weeks old were studied. A completely randomized experimental design in factorial arrangement, with 6 treatments of 6 replicates with 9 hens each, was applied. Treatments consisted of 2 production cycles (80 and 108 weeks) and three body weight losses during moulting (20%, 25%, and 30%). Productive performance (body weight, feed intake, egg weight, production percent, egg mass, feed …

Molecular Characterization Of Bacillus Anthracis Isolates Recovered From Nomic Andnonnomic Hosts, Doğan Akça, Fati̇h Büyük, Emre Karakaya, Mustafa Reha Coşkun, Eli̇f Çeli̇k, Mi̇tat Şahi̇n, Özgür Çelebi̇, Sali̇h Otlu, Ali̇ye Gülmez Sağlam, Eray Büyük, Seda Durhan, İzzet Burçi̇n Saticioğlu, Seçi̇l Abay, Tuba Kayman, Fuat Aydin

Turkish Journal of Veterinary & Animal Sciences

Anthrax, which is primarily a disease of herbivores, is known to infect many animal species. In order to break the cycle of anthrax infection, it is important to identify the potential hosts or reservoirs of its causative agent. Therefore, this study aimed to characterize Bacillus anthracis isolates recovered from a variety of hosts and environments during anthrax sporadic in the Kars and Kayseri regions of Turkey. For this purpose, a total of 23 B. anthracis field isolates obtained from human, cattle, sheep, dog, horse, puma, soil, and fodder were used. In addition, the Sterne vaccine strain was included. All B. …

Determination Of Plant-Specific Retrotransposons In Chicken, Levent Mercan, Ci̇hat Erdem Bülbül, Fati̇h Bi̇lgi̇, Sevgi̇ Marakli

Turkish Journal of Veterinary & Animal Sciences

Mobile genetic elements are also known as transposons, comprising a significant proportion of plant and animal genomes. Retrotransposons, a class of transposons, move copy and paste mechanism and, therefore, cause genome obesity. Moreover, the transferability of retrotransposons within and between different kingdoms has provided valuable information for evolutionary relationships in addition to detailed knowledge about genomes. We identified barley-specific retrotransposons (Nikita and Sukkula) and determined polymorphism ratios of these retrotransposons in a chicken genome for the first time to gain new insights for retrotransposons found in different genomes. Transposon sequences belonging to chicken and barley genomes were also investigated to …

Epidemiological Investigation Of Bovine Tuberculosis Infection Dynamics In Turkey, Şahi̇n Çakir, Murat Yildirim, Kadi̇r Serdar Di̇ker, Fevzi̇ye İpek Keski̇n, Selcen Yüksel, Burak Deveci̇, Erhan Akçay

Turkish Journal of Veterinary & Animal Sciences

A national epidemiological research project was carried out to define the dynamics affecting the epidemiology of bovine tuberculosis (bTB) infection in Turkey and to identify the risk factors. Official veterinarian (OV) and breeder original questionnaires were produced separately as part of this study to collect thorough data regarding the disease from the field. The number of questionnaires that needed to be filled out was decided by 95% confidence interval (CI) and 5% margin of error. The findings of 371 OV and 317 breeder questionnaires completed online across the country were analyzed. In addition, 28 outbreaks determined by random method were …

Estimation Of The Factors Affecting Lactation Milk Yield Of Holstein Cattle By The Adaptiveneuro-Fuzzy Inference System, Aycan Mutlu Yağanoğlu

Turkish Journal of Veterinary & Animal Sciences

The aim of this study is to estimate lactation milk yield of Holstein cattle using first calving age, lactation period, and service period with (ANFIS) adaptive neuro-fuzzy inference system. The input variables for the system in the study were first calving age, lactation period, and service period. The output variable from the system was lactation milk yield. Predicted values obtained from the ANFIS were compared with the observed values. Twenty-seven rule functions are used to obtain the best model and 1000 epochs are used to estimate the accuracy of the training and testing error. The relations between the output and …

The Effects Of Rations Containing Ad Libitum Limestone Grit With Whole Flaxseed On Quails?Performance, Egg Quality, And Blood Parameters, Kadi̇r Emre Buğdayci, Fatma Karakaş Oğuz, Mustafa Numan Oğuz, Cafer Mercan

Turkish Journal of Veterinary & Animal Sciences

The purpose of this research is to determine the effects of consuming feed rations containing ad libitum limestone grit with whole or ground flaxseed (10%) on quails? performance, egg quality (shape index, albumen index, yolk index, and Haugh unit), and some blood parameters (hematocrit, cholesterol, and triglyceride) during a period of 8 weeks. Five weeks old quails (Coturnix coturnix japonica; 140 in total) were divided into control and 4 trial groups. The rations of the first (W) and second (G) trial groups consisted of whole or ground flaxseeds (10%), respectively. The composition of the rations of the third (Wg) and …

Development Of Two Novel Sybr Green-Based Quantitative Pcr Assays For Detection Andquantification Of Mycoplasma Gallisepticum And Mycoplasma Synoviae, Mohsen Bashashati, Fereshteh Sabouri

Turkish Journal of Veterinary & Animal Sciences

Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are considered the most pathogenic and economically devastating avian mycoplasmas. A definite MG and MS diagnosis depends on three main approaches, including isolation and identification, demonstration of specific antibodies, and detection of genetic materials. A fast and precise diagnosis technique is an indispensable prerequisite to prevent and control avian pathogens more effectively. This study aimed to develop and validate convenient SYBR green-based quantitative PCR (qPCR) assays using genome specific primers to rapidly detect and quantify MG and MS. Two sets of primers were designed based on the highly conserved regions of mgc2 and …

The Effects Of Upgrading And Intersecting Cross-Breeding Strategies On The Geese Economic Traits, Yujian Sui, Qiong Liu, Chenguang Xu, Wei Yang

Turkish Journal of Veterinary & Animal Sciences

Routine, upgrading, and intersecting cross-breeding strategies are three methods frequently used in poultry breeding. But, till now, comparative assessment of the effects on the improvement of geese economic traits with these strategies is largely unknown. Here, using Carlos goose and Zi goose, these three strategies were employed and compared. The main economic traits, including egg production capability, egg quality, growth performance, meat production capability, and down feather production capability were all measured systematically. For egg production capability and egg quality, the upgrading group had the highest egg-laying rate, and the intersecting group had the biggest egg size compared with the …

Effects Of Dietary Calcium, Phosphorus And Microbial Phytase On Intestinal Morphologyin Laying Hens, Sabi̇re Güler, Ender Deni̇z Asmaz, Nuray Varol Kayapunar, İhsan İşbi̇li̇r, Şeri̇fe Şule Cengi̇z, Derya Yeşi̇lbağ, Ahmet Batuhan Şanli, Eyüp Eren Gültepe

Turkish Journal of Veterinary & Animal Sciences

Different challenges are being applied in the poultry industry in order to protect animal health and to increase immunity and production. The supplementation of microbial phytase is essential in terms of both reducing the inorganic phytase rate and contributing to the absorption of other minerals. In this study, a newly isolated microbial phytase was added at different concentrations to the diet together with calcium (Ca$^{2+}$) and available phosphorus (AP), and the effects of this supplementation on intestinal absorption capacity and Ca$^{2+}$ binding capacity were investigated via morphological measurements and immunohistochemical examination of the duodenum and ileum. For this purpose, 90 …

Anatomical And Histological Structures Of Eye And Lacrimal Gland In Norduz Andmorkaraman Sheep, Semi̇ne Dalga, Serap İlhan Aksu, Kadi̇r Aslan, Turgay Deprem, Reşi̇t Uğran

Turkish Journal of Veterinary & Animal Sciences

The current study was conducted to determine the morphological, morphometric, and histological characteristics of bulbus oculi and lacrimal gland in sheep. For this purpose, 28 pairs (n = 7 males and n = 7 females) of bulbus oculi from Morkaraman and (n = 7 males and n = 7 females) Norduz sheep were analyzed. The bulbus oculi in the skulls were dissected from the orbit and removed. The morphometric data such as distance between endpoints of the dissected bulbus oculi in temporal, nasal, and dorsal directions, the length between endpoints of the pupil in dorsal and ventral directions, and as …