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Articles 56671 - 56700 of 255951

Full-Text Articles in Life Sciences

Function And Expression Of Ribonucleotide Reductase-Encoding Genes In Flavobacterium Johnsoniae, Hunter Scott Doheny Jan 2022

Function And Expression Of Ribonucleotide Reductase-Encoding Genes In Flavobacterium Johnsoniae, Hunter Scott Doheny

All Graduate Theses, Dissertations, and Other Capstone Projects

Ribonucleotide reductases (RNRs) are essential enzymes that are involved in DNA synthesis. While this makes them ideal targets for antiproliferative drugs, most known RNR targeting agents are nonspecific. This poses a problem when considering antimicrobials, but several groups of bacteria have RNRs that utilize unique mechanisms that could be exploited if fully understood. One such bacteria, Flavobacterium johnsoniae, has the gene for a class Id RNR with a novel activation mechanism. Class Id RNRs belong to the aerobic class I RNRs and seem to have a unique metallocofactor and activation mechanism involving superoxide and manganese. The goal of this research …

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Development Of Genetic Manipulation Techniques For The Fish Pathogen Flavobacterium Psychrophilum, Seada Sloboda Jan 2022

Development Of Genetic Manipulation Techniques For The Fish Pathogen Flavobacterium Psychrophilum, Seada Sloboda

All Graduate Theses, Dissertations, and Other Capstone Projects

Flavobacterium psychrophilum is a known fish pathogen, causing bacterial cold-water disease (BCWD) and rainbow trout fry syndrome (RTFS), predominantly in salmonids. The disease causes tissue damage and tail rot in young and adult fish. The issue is prevalent in fisheries in the Pacific Northwest and the treatments often entail the use of antibiotics. Genetic manipulations in F. psychrophilum are scarce, due to most of the strains’ ability to destroy foreign DNA via restriction enzymes. The virulence mechanisms of F. psychrophilum are not well understood. Lack of genetic manipulation tools in F. psychrophilum has also hampered the development of live attenuated …

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Environmental Effects On Biomass Allocation And Small Plot Evaluations Of Aquatic Pesticides For Control Of Nitellopsis Obtusa (Starry Stonewort) Collected From Lake Koronis In Stearns County, Minnesota, Patrick J. Carver Jan 2022

Environmental Effects On Biomass Allocation And Small Plot Evaluations Of Aquatic Pesticides For Control Of Nitellopsis Obtusa (Starry Stonewort) Collected From Lake Koronis In Stearns County, Minnesota, Patrick J. Carver

All Graduate Theses, Dissertations, and Other Capstone Projects

Phenology is the study of critical life stages in plants in relation to shifts in environmental factors during seasonal changes. Starry stonewort is a green macro alga in the family Characeae native to Eurasia. Much of the Midwestern United States has been invaded by this species. Starry stonewort has been seen to have late season growth patterns in its invaded range. This study found that this growth pattern was dependent upon water temperature and light transmittance both of which affect biomass production and senescence. It was hypothesized that peak biomass would occur in the late summer (August), however we observed …

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Sheepshead (Archosargus Probatocephalus) Neurocranium, Andrew J. Hartmann, Steven Peterson, David W. Kerstetter Jan 2022

Sheepshead (Archosargus Probatocephalus) Neurocranium, Andrew J. Hartmann, Steven Peterson, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from Sheepshead (Archosargus probatocephalus)

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A New Achillea (Asteraceae) Species From Southwestern Turkey, Gürkan Semi̇z, Tuna Uysal, Meryem Bozkurt, Batikan Günal Jan 2022

A New Achillea (Asteraceae) Species From Southwestern Turkey, Gürkan Semi̇z, Tuna Uysal, Meryem Bozkurt, Batikan Günal

Turkish Journal of Botany

Achillea alimeana Semiz & Uysal sp. nov. of sect. Santolinoidea DC. (Asteraceae), was described, diagnosed and illustrated as a new species. It was collected from Akdağ Mountains in Denizli Province. In this paper, the diagnostic characters of A. alimeana and its similar species were discussed in the morphological, palynological and molecular findings. As regards the new species, some notes on the ecology, conservation status, and distribution were submitted. A diagnostic key was given for related taxa.

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The Impact Of Planting Season And Crop Residue On Germination, Reproductive Success, And Mass Of Native Forbs, Michaela Vonlintel Jan 2022

The Impact Of Planting Season And Crop Residue On Germination, Reproductive Success, And Mass Of Native Forbs, Michaela Vonlintel

Master's Theses or Doctor of Nursing Practice

The lack of biodiversity in prairie restorations compared to native prairies is alarming, and restoring this diversity has been a key focus of research and restoration projects for years. This study aims to assess two variables: planting season and plant residue, for achieving success in forb establishment. This research was conducted in a greenhouse using mesocosms that were seeded in spring, summer, and fall with nine forb species. Half of each seasonal treatment received ground cover, while the other half did not. Two hypotheses were formed. The first was that the fall planting will be the most successful seasonal treatment …

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Beneficial Plant-Microbe Interactions To Improve Nutrient Uptake And Biotic Stress Response In Crops, Jaya Krishna Yakha Jan 2022

Beneficial Plant-Microbe Interactions To Improve Nutrient Uptake And Biotic Stress Response In Crops, Jaya Krishna Yakha

Electronic Theses and Dissertations

Mutualism is a very common phenomenon among living organisms on earth. Legumes because of their high protein content, serve as a great nutrient resource for animals. This group of plants can form a mutualistic symbiosis with beneficial microbes. For example, Alfalfa (Medicago) and soybean (Glycine max) can get colonized with arbuscular mycorrhizal fungi (AMF) and rhizobia bacteria simultaneously forming a complex tripartite interaction for nutrient benefits. Most of the previous research evaluated individual symbionts, either rhizobia bacteria or AMF, but not both. There are only a few reports which discuss the nutrient exchange mechanisms in a tripartite interaction. Thus, there …

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Evaluating Fungicide Timing, Efficacy, And Sensitivity As Well As Candidate Resistance Genes Against Fungi Causing Phomopsis Stem Canker In Sunflower, Ruchika Kashyap Jan 2022

Evaluating Fungicide Timing, Efficacy, And Sensitivity As Well As Candidate Resistance Genes Against Fungi Causing Phomopsis Stem Canker In Sunflower, Ruchika Kashyap

Electronic Theses and Dissertations

Phomopsis stem canker is one of the major diseases of sunflower (Helianthus annuus L.) caused by complex of Diaporthe spp., in the U.S. This disease became of economic importance after 2010 epidemic in Minnesota, North Dakota, and South Dakota and since then the prevalence of this diseases has increased due to increased average precipitation in these major sunflower growing regions of the U.S. The two important disease management strategies involve the use of fungicides and partially resistant hybrids. However, over the years, researchers and growers have seen varied results on Phomopsis stem canker disease severity reduction and yield gains, when …

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Optimization Of Forage Use In Diets Fed To Backgrounding And Finishing Cattle In The Northern Great Plains, Thomas G. Hamilton Jan 2022

Optimization Of Forage Use In Diets Fed To Backgrounding And Finishing Cattle In The Northern Great Plains, Thomas G. Hamilton

Electronic Theses and Dissertations

The two studies in this thesis were conducted to: 1) investigate the impact of corn silage moisture content and kernel processing at harvest on growth performance, efficiency of dietary net energy utilization, and carcass traits in finishing steers when fed at 20% DM inclusion in diets containing modified distillers grains plus solubles; and 2) determine the influence of equal cumulative roughage inclusion in a single diet or twodiet system during a 210-d backgrounding-finishing period in pre-conditioned beef steers on growth performance responses, efficiency of dietary net energy (NE) utilization, and carcass traits. Experiment 1 was a 112-d finishing experiment conducted …

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Design Of Affordable Polyaniline - Based Wearable Sensor For Real Time Plant Growth Monitoring, Temitope Borode Jan 2022

Design Of Affordable Polyaniline - Based Wearable Sensor For Real Time Plant Growth Monitoring, Temitope Borode

Electronic Theses and Dissertations

The need for precision agriculture in today and future farming cannot be overstressed. Recent agriculture is characterized by numerous challenges such as environmental stressors and resource constraints resulting in low crop yield and unsustainable agriculture practices. Understanding plant growth mechanism and growth rate under the external influence is a bedrock towards precision agriculture practices ensuring optimum yield and sustainable utilization of resources. The current study addresses this need by developing and applying an affordable and stable sensor for monitoring plant growth. The sensor was fabricated by in-situ chemical polymerization of aniline on an elastic band substrate by dip coating. The …

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Defining Amino Acid Requirements Of Pregnant Sows: Challenges And Opportunities, Christian D. Ramirez-Camba Jan 2022

Defining Amino Acid Requirements Of Pregnant Sows: Challenges And Opportunities, Christian D. Ramirez-Camba

Electronic Theses and Dissertations

The efficient use of protein in animal production is dependent on the protein supply and its constituent amino acids (AA) in relation to the animal's needs. Excess AA are deaminated, and the resulting nitrogen (N) is excreted, whereas suboptimal AA intake reduces animal performance. Both increased nutrient excretion and decreased animal performance reduce the overall efficiency of the production unit. In sows, AA requirements should be adequate for optimizing reproductive performance, as measured, for example, by the number of pigs produced per sow per year while limiting N excretion. There is a desire to feed pregnant sows AA levels that …

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Above-Ground Biomass Estimation In Oat Breeding Nurseries Using Uav-Based Multispectral Data, Rakshya Dhakal Jan 2022

Above-Ground Biomass Estimation In Oat Breeding Nurseries Using Uav-Based Multispectral Data, Rakshya Dhakal

Electronic Theses and Dissertations

Conventional methods of phenotyping for above-ground biomass under field conditions are time consuming, costly, and labor intensive. Unmanned Aerial Vehicles (UAV) offer a rapid and non-destructive approach for collecting high temporal and spatial resolution imagery of multiple field plots. Vegetation indices (VIs) can be derived from those imagery and have been used broadly for high-throughput biomass estimation, however, they suffer from spectrum saturation issues at high canopy density. In addition, VIs only reflect the canopy spectral information and fail to incorporate the 3-dimensional (3D) canopy structure and architecture information. It also ignores the spatial relationship of pixels to each other …

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Factors Influencing Mortality Of Stocked Rainbow Trout In Black Hills Reservoirs, Charles A. Mordhorst Jan 2022

Factors Influencing Mortality Of Stocked Rainbow Trout In Black Hills Reservoirs, Charles A. Mordhorst

Electronic Theses and Dissertations

Because return to angler is an important outcome of put-and-take fisheries, understanding mortality of stocked Rainbow Trout is fundamental to managing these fisheries. Harvest rates of stocked Rainbow Trout Oncorhynchus mykiss in the Black Hills of South Dakota are believed to be below management objectives. Rainbow Trout not harvested by anglers are assumed to be lost to various sources of mortality, raising concerns about the cost of the Rainbow Trout stocking program relative to the benefit provided to anglers. (Simpson 2008). This study evaluated the factors influencing mortality of Rainbow Trout stocked into Black Hills reservoirs. We assessed the effects …

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Optimization Of Electrically Driven Fluid Flow Through Tissue Hydrogels, Joseph C. Walker Jan 2022

Optimization Of Electrically Driven Fluid Flow Through Tissue Hydrogels, Joseph C. Walker

Electronic Theses and Dissertations

No abstract provided.

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Identification And Characterization Of Candidate Rumen Bacterial Species From Beef Cattle With The Potential For Metabolizing Lipids, Cheyenne E. Hron Jan 2022

Identification And Characterization Of Candidate Rumen Bacterial Species From Beef Cattle With The Potential For Metabolizing Lipids, Cheyenne E. Hron

Electronic Theses and Dissertations

Ruminants and the microorganisms within their rumen have an intricate symbiotic relationship. Ruminants are able to convert plant materials that are indigestible to humans into meat, milk, and fiber for human consumption and use. Supplementing lipids in diets fed to beef cattle could be beneficial for the host animal as lipids are a higher density energy source. However, they can be detrimental to rumen microorganisms. While much research has been involved in elucidating the composition of the ruminal microbiome, knowledge of the effects of lipids on the ruminal microbiome has been quite limited. In this context, we took advantage of …

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Evaluating Avian Use Of Cover Crops In The Corn Belt, Megan Figura Jan 2022

Evaluating Avian Use Of Cover Crops In The Corn Belt, Megan Figura

Electronic Theses and Dissertations

The tallgrass prairie of North America has changed drastically since colonial settlement, with up to 99% of this region converted for agriculture and other land-uses. Concurrent with grassland conversion, grassland birds have experienced the most extreme, consistent, and widespread population declines of any avian guild. Agricultural lands in the U.S. Midwest were able to provide adequate habitat for several bird species until the 1950’s; however, altered and intensified management practices have degraded much of remaining suitable habitat and undermined ecosystem functions. Consequently, many grassland birds have been identified as Species of Greatest Conservation Need (SGCN) in many State Wildlife Action …

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Pathogen Prevalence In Domestic Sheep In Western Nebraska: Implications For Bighorn Sheep Conservation And Coexistence On A Multi-Use Landscape, Kaytlin Bohr Jan 2022

Pathogen Prevalence In Domestic Sheep In Western Nebraska: Implications For Bighorn Sheep Conservation And Coexistence On A Multi-Use Landscape, Kaytlin Bohr

Electronic Theses and Dissertations

Bighorn sheep (Ovis canadensis) and domestic sheep (Ovis aries) are members of the same genus and thus share multiple pathogens that can be spread between them. One specific respiratory pathogen of concern is Mycoplasma ovipneumoniae (M. ovipneumoniae), which has been linked to pneumonia epizootics in bighorn sheep that are often characterized by all age die offs upon initial exposure followed by years of low lamb recruitment. Domestic sheep have been identified as one carrier of M. ovipneumoniae with transmission between sheep species occurring when there is close contact on the landscape. To prevent this cross-species transmission, importance has been placed …

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Preparation And Characterization Of Cellulose-Based Biodegradable Films From Switchgrass And Spent Coffee Grounds, Sajal Bhattarai Jan 2022

Preparation And Characterization Of Cellulose-Based Biodegradable Films From Switchgrass And Spent Coffee Grounds, Sajal Bhattarai

Electronic Theses and Dissertations

Plastic packaging materials, which take more than 700 years to degrade, have polluted land, water, and the atmosphere. The consequent environmental and life hazards require attention to alleviate plastic concerns. Developing plastic-replacing materials could be a viable option. To this end, lignocellulosic fraction from inexpensive and widespread agricultural biomass switchgrass (SG) and biowaste spent coffee grounds (SCG) offer a possible solution, mainly to prepare biodegradable packaging films. Herein, lignocellulosic fraction from SG and SCG has been extracted, solubilized in ZnCl2 solution, and crosslinked through Ca2+ ions to prepare gels and cast biodegradable films. The SG was subjected to three extraction …

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To Study The Factors Influencing The Sampling Variability On Microbiological Analysis Of Milk And Whey, Ratul Kalita Jan 2022

To Study The Factors Influencing The Sampling Variability On Microbiological Analysis Of Milk And Whey, Ratul Kalita

Electronic Theses and Dissertations

Sampling and analysis are integral parts of dairy processing in quality control, process monitoring and control, and meeting regulatory standards. Accurate sampling is vital to avoid erroneous analysis results and interpretation. The sampling process depends on the appropriateness of sample collection, transportation, storage, and analysis conditions. The bacterial heterogeneity and cluster formation, structural heterogeneity of food, sample unit and size, frequency, and sampling methods, are also crucial in sampling. We aimed to evaluate the influence of sample volume, sampling frequency, and sampling methods in experimental processing lines of whole milk, skim milk, and whey. To standardize the experimental protocols, a …

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A Process To Produce Lactose Phosphate From Dairy Byproducts And Used As An Alternative To Emulsifying Salts In Processed Cheese Food Manufacture, Khalid Ahmed Alsaleem Jan 2022

A Process To Produce Lactose Phosphate From Dairy Byproducts And Used As An Alternative To Emulsifying Salts In Processed Cheese Food Manufacture, Khalid Ahmed Alsaleem

Electronic Theses and Dissertations

Lactose is the primary carbohydrate in most mammals' milk, commonly known as milk sugar. Milk permeate is a by-product of whey protein manufacturing through membrane technologies. It is cost-effective, available, and an excellent source of lactose. Sugar phosphorylation is a technique used to alter sugar's characteristics. It has numerous applications for developing dairy and food products. Lactose-6-phosphate (LP) is an organic compound that switches the hydrogen on lactose by monophosphate that has the potential to function as emulsifying salts (ES). ES, such as disodium phosphate (DSP) and trisodium citrate, have a critical effect on the emulsification characteristics of casein by …

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Beta Glucan Enriched Oat-Wheat Naan - Nutritional, Sensory, And Rheological Evaluation Of Par-Baked Leavened Flatbreads, Anjana Adhikari Jan 2022

Beta Glucan Enriched Oat-Wheat Naan - Nutritional, Sensory, And Rheological Evaluation Of Par-Baked Leavened Flatbreads, Anjana Adhikari

Electronic Theses and Dissertations

No abstract provided.

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Development Of Genomic Resources In Vitis Riparia For Discoveries On Pre- And Post-Transcriptional Molecular Regulators Of Early Induction Into Endodormancy, Michael Robben Jan 2022

Development Of Genomic Resources In Vitis Riparia For Discoveries On Pre- And Post-Transcriptional Molecular Regulators Of Early Induction Into Endodormancy, Michael Robben

Electronic Theses and Dissertations

Grapevine is one of the most important fruit crops in the world, responsible for billions in global sales annually. The largest threat to grapevine and other crop production is global climate change resulting human activities. This brings unpredictable and drastic changes in ambient air temperatures to many climates in which grapes are grown. Lower temperatures and inclement weather are already responsible for millions in lost revenue due to tissue damage of established plants. Thus, protecting grapevine crops from weather-related damage is the biggest concern to growers aside from pathogen- and diseaserelated crop damage. The primary mechanism for winter survival in …

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Microbial Communities And Nitrogen Dynamics In Prairie And Cropland Soils, Bikram Kumar Das Jan 2022

Microbial Communities And Nitrogen Dynamics In Prairie And Cropland Soils, Bikram Kumar Das

Electronic Theses and Dissertations

Soil microbial communities are affected by many factors, such as soil nitrogen (N) and the quantity of grass-root exudates, changing seasons, fertilization method, plant diversity, and their origin. This holds for both natural and fertilized soils. This research was intended to expand the current understanding of soil microbial interactions associated with ecological functions. The objectives of the research were (I) to characterize prokaryotic soil communities, estimate functional potential, and quantify nitrogen cycle-associated prokaryotic gene expressions across three phases of the growing season, (II) to explore the diazotrophic community composition in a natural system using nifH sequencing as well as 16S …

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Managing Water Quantity And Quality With Subsurface Drainage In Eastern South Dakota, Morghan Hurst Jan 2022

Managing Water Quantity And Quality With Subsurface Drainage In Eastern South Dakota, Morghan Hurst

Electronic Theses and Dissertations

Moisture extremes (excess and deficit) impact crop loss and water quality. Due to excessively wet springs and dry summers, crop damage can occur within the same county or field within the same year. To determine the magnitude of this problem in eastern South Dakota, indemnified crop insurance data for drought and excessive moisture claims were assessed for the years 1991-2020 for the occurrence of both excess moisture and drought in four counties in eastern South Dakota. Results show that there is greater than a 70% chance of the moisture extremes happening in the same year, making subsurface drainage, controlled drainage, …

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Exploring Bioprocessing Technologies For Diverse Industrial Application Of Canola, Ahmad Fawzi N Alhomodi Jan 2022

Exploring Bioprocessing Technologies For Diverse Industrial Application Of Canola, Ahmad Fawzi N Alhomodi

Electronic Theses and Dissertations

Globally, canola is the second largest oilseed crop after soybean, which is processed for the commercial production of high value oil. The industrial processing steps for canola oil extraction include preprocessing (cleaning, pressing, flaking, and cooking), mechanical pressing, and/or organic solvents. This process results in large quantities of protein-rich (~ 40% dry basis) meal as a co-product. The meal is used partially in animal diet (<30% inclusion) due to high levels of antinutritional factors (ANFs) such as high fibers, phytic acid, and glucosinolates, and low metabolizable energy. Thus, this research was designed to examine various bioprocessing technologies (i.e., traditional sprouting, solid state fermentation, submerged state fermentation, co-culture fermentation, mild pretreatments, and their combinations) for possible advancement in canola use. Canola seed sprouting for 6-day period led to an increase in protein content and a reduction in ANFs and oil content of sprouts compared to ungerminated seed. Subsequent submerged state fermentation of 6- day old sprouts using three different strains (Aureobasidium pullulans, Trichoderma reesei and Neurospora crassa) further increased protein content and lowered ANFs. Solid-state fermentation of 144 h old sprouts using A. pullulans, N. crassa and T. reesei enhanced the protein content and reduced ANFs of sprouts. Sprouting canola seed for three days helped in hull removal, leading to high protein meal accompanied by low fiber and phytic acid level. Three-day seed sprouting had no effect on oil yield, but free fatty acid content was higher compared to seed oil. Co-culture fermentation of HECM under solid state process showed maximum reduction in fiber content with co-culture of A. pullulans and N. crassa while a combination of A. pullulans and T. reesei promoted the highest GLS and phytic acid reductions compared to other combinations, which indicated the advantage of coculture inoculation over monoculture in terms of ANFs reduction. Mild pretreatment of HECM using deionized water resulted in washed HECM (WHECM) with lower soluble sugars and GLS compared to untreated HECM, whereas protein and amino acid were concentrated due to the removal of soluble components. WHECM compared to HECM showed higher protein digestibility when fed to rainbow trout. Subsequent mono- and coculture fermentation of HECM and WHECM under submerged process resulted in higher protein and amino acid content and lower ANFs levels compared to uninoculated controls. The results of cellulase, endoglucanase and β-glucosidase activity indicated the crucial role of used substrates, fungi, fermentation modes (solid state/submerged stated) and inoculation methods (mono-/co-culture) on enzyme activities.

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Antibacterial Activity Of Commiphora Molmol (Myrrha) Against The Yeast, Mohammad Hassan Alshehri Jan 2022

Antibacterial Activity Of Commiphora Molmol (Myrrha) Against The Yeast, Mohammad Hassan Alshehri

Selected Full-Text Master Theses 2021-

Commiphora molmol (myrrha) is widely used as a traditional medicine around the globe against inflammatory diseases. It is also considered to be effective as anti-fungal, anti-cancer and anti-oxidant agent. The minimum inhibitory concentration, bactericidal activity and heat stability of antibiotic component in myrrha oil extract has been investigated. The MIC was determined in nutrient free buffer and in nutrient rich peptone glucose buffer. The results showed that MIC was 0.6% in nutrient free buffer as no growth was shown by cells, whereas, in peptone glucose media a slight increase in cell count was seen. It can be concluded that it …

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Assessment Of Genetic Variation Of Natural Populations Of Wild Cherry (Prunus Avium L.)Via Ssr Markers, Burcu Uzan Eken, Emrah Kirdök, Ercan Veli̇oğlu, Yelda Özden Çi̇ftçi̇ Jan 2022

Assessment Of Genetic Variation Of Natural Populations Of Wild Cherry (Prunus Avium L.)Via Ssr Markers, Burcu Uzan Eken, Emrah Kirdök, Ercan Veli̇oğlu, Yelda Özden Çi̇ftçi̇

Turkish Journal of Botany

Wild cherry (Prunus avium L, syn. Cerasus avium L. Moench.) is a widely spread forest tree that has ecological and economical importance. However, the genetic diversity of this species is threatened for many reasons. Therefore, a breeding and conservation program should be established in order to minimise the loss of genetic diversity. In this study, we aimed to understand the genetic structure of 440 individual wild cherries sampled from 22 different populations in Turkey using 10 SSR molecular markers. With the molecular variance analysis, we found that the genetic diversity within the population is approximately 88.5% and the genetic diversity …

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Mining And Analysis Of Chloroplast Simple Sequence Repeats (Ssrs) From Eight Species Of Aquilaria, Muhammad Syahmi Hishamuddin, Ahmad Syazwan Samsuddin, Rozi Mohamed Jan 2022

Mining And Analysis Of Chloroplast Simple Sequence Repeats (Ssrs) From Eight Species Of Aquilaria, Muhammad Syahmi Hishamuddin, Ahmad Syazwan Samsuddin, Rozi Mohamed

Turkish Journal of Botany

Aquilaria is a tropical forest tree, producer of the famed and expensive agarwood. Aggressive collection of agarwood put strain on the natural stands of Aquilaria species, sparking efforts to domesticate the tree and cultivate agarwood in plantations. However, tree domestication progress is hampered by the scarcity of genomic resources that is crucial for breeding programs. In this study, the complete chloroplast (cp) genome sequences from eight Aquilaria species were analyzed in silico. For identification of the simple sequence repeats (SSRs), MISA PERL script which had a repeat length of 12 for mononucleotides (mono-), 6 for dinucleotides (di-), 4 for trinucleotides …

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Karyomorphology, Ploidy Analysis, And Flow Cytometric Genome Size Estimation Of Medicago Monantha Populations, Homa Zarabizadeh, Ghasem Karimzadeh, Sajad Rashidi Monfared, Saeed Tarkesh Esfahani Jan 2022

Karyomorphology, Ploidy Analysis, And Flow Cytometric Genome Size Estimation Of Medicago Monantha Populations, Homa Zarabizadeh, Ghasem Karimzadeh, Sajad Rashidi Monfared, Saeed Tarkesh Esfahani

Turkish Journal of Botany

Karayomorphological analysis and monoploid genome size (2Cx DNA) of 12 Iranian Medicago monantha populations are reported. Flow cytometric analysis were conducted on freshly collected seeds, using Solanum lycopersicum cv. Stupicke (2C DNA = 1.96 pg), as the internal reference standard. All populations were diploid with variable degrees of mixoploidy and two different chromosome numbers within each population. Totally, six chromosome numbers of 22, 28, 30, 32, 36, and 40 were identified. Twotyped chromosomes ("m", "sm") with a mean length of 1.65 µm (0.98-2.99 ?m) formed seven different karyotype formulas. Karyotypes were mostly symmetrical, implying the evolutionary effect of natural and …

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Current Nomenclature And Systematics Of Capsella Medik. With Lectotypifications:Towards Solving The Puzzle, Yelda Güzel Jan 2022

Current Nomenclature And Systematics Of Capsella Medik. With Lectotypifications:Towards Solving The Puzzle, Yelda Güzel

Turkish Journal of Botany

The taxonomic history of Capsella Medik., one of the genera with the highest phenotypic plasticity, and thus the highest synonym number relative to the recognized species number, was reviewed. The current systematics of the genus was discussed and the exact number of taxa was determined. In light of the molecular and nomenclatural data, it was decided that the genus was represented around the world by five species and one hybrid. Considering the molecular studies that have been conducted over the 30 years it was concluded that the species of this genus are typical examples of the concept of cryptic species. …

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