Life Sciences Commons^™

Extending Plasma Proteome Coverage To Enable Biomarker Discovery, Jordan Bruce Burton

Wayne State University Dissertations

Two features make plasma a valued source of health information: First, plasma contains secreted proteins from all tissues in the body and can, therefore, provide information about the health status of all tissues. Second, plasma is available in large quantity using a minimally invasive procedure that is well tolerated. The status of plasma as a valued source of health information is matched by the difficulty in extracting proteome information from the samples. Using state of the art instrumentation a standard analysis of plasma yields quantitative information on approximately 300 proteins. Extreme fractionation strategies coupled with increased mass spectrometry time extends …

Preventative Weed Management Strategies In Arkansas Tomato Production, Gracie E. Morrison, Matthew Bertucci

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Cost-effective weed suppression is an important consideration for tomato growers. Growers often choose methods which minimize hand labor, as hand weeding can be prohibitively expensive. This project determined economic viability of high tunnel tomatoes treated with several methods of weed control, both organic and chemical. These methods included: 2-week hand weeding, 1-week hand weeding, preemergent herbicide application (plots sprayed with herbicide prior to weed emergence), straw mulch (plots bedded with straw), landscape fabric (plots covered in fabric), and untreated weedy control plots. These six treatments were applied to randomized blocks in a high tunnel. Weeding, planting, and harvesting were all …

Using Acid-Detergent Insoluble Ash As An Internal Marker To Compare Digestibility And Fecal Output By Ovine That Were Offered Hays Of Varying Nutrient Composition, Kennedy Barnett, Trisha Adams, Kenneth P. Coffey, Kathleen S. Jogan

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Digestibility markers are used to determine total digestibility without collecting total feces, which reduces time and labor and allows greater numbers of animal observations to be obtained. The goal was to determine if the digestibility marker acid detergent insoluble ash (ADIA) was fully recovered, and based on that information, determine the accuracy of total digestion predictions using ADIA. Different proportions of sericea lespedeza hay and alfalfa silage were offered to 16 gestating Dorper ewe lambs (Ovis aries) (41.8 ± 4.61 kg body weight) to determine digestibility. Total feed intake and fecal excretion were measured during 2 experimental periods consisting of …

Review Of Spiders Of North America, By Sarah Rose, Michael L. Draney

The Prairie Naturalist

A field guide to spiders, the dominant terrestrial predator group, has long been a desire for North American naturalists, biologists, and spider fans, but not surprisingly, the first “real” field guide has been a long time coming. The diversity of spiders, with over 50,000 known species globally (World Spider Catalog 2022) and around 4,000 in North America north of Mexico (Rose 2022), is well beyond the diversity of more traditional field guide targets such as birds (about 1,000 species north of Mexico; American Birding Association 2022) or butterflies (about 725 species north of Mexico; North American Butterfly Association 2022). Also, …

Review Of Methods For Ecological Research On Terrestrial Small Mammals, By Robert Mccleery, Lynda R. Lafond

The Prairie Naturalist

Small mammals are used in ecological studies throughout the world, yet the subject has lacked a clear reference that included upto- date methods pertaining to passive detection, molecular, and statistical approaches. In Methods for Ecological Research on Terrestrial Small Mammals, McCleery and his coauthors build on previous research references (Braun 2005, Sutherland 2006, Krebs 2014, Silvy 2020) and present methodologies specific to the study of small mammals that are applicable globally. Written as an asset for novice and experienced small mammal researchers alike, Methods for Ecological Research on Terrestrial Small Mammals is organized in a logical format, providing readers …

Candida Auris: Emergence Of A Multi-Drug Resistant Fungal Pathogen, Garrett Bryak, Brooke Tharp, Rachel Zheng

Discovery Undergraduate Interdisciplinary Research Internship

Candida auris is an emerging fungal pathogen that poses a significant public health concern due to the species’ increasing resistance to a vast array of FDA-approved antifungal drugs and high mortality rate. The pathogenic fungus has also proven difficult to identify within a conventional diagnostic laboratory and has been responsible for numerous outbreaks in healthcare settings across the globe, as noted by the Center for Disease Control and Prevention (source). This research effort aims to provide a comprehensive review detailing the history, origin, and biological characteristics of C. auris with particular emphasis on analyzing the nature of C. auris’s multidrug-resistant …

Fair Resource Allocation To Reduce Inequalities In Criminal Justice & Healthcare, Alex Liu, Alexia Rodrigues

Discovery Undergraduate Interdisciplinary Research Internship

Together, we are working to identify discrepancies in the criminal justice system that put substance users at an unfair advantage for possible treatment and success in the future. This is done by modeling regressions involving the arrest-rates, developing charts to show the patterns of past arrests, and tracking the resources available for substance users. By doing this, we provide models to the local sheriff office that forecasts incoming inmates which allows the office to prepare accordingly. On the flip side, we are working to create a prediction model that exemplifies the risk of relapse for substance users. Ultimately, our goal …

West Virginia Herbaria: Status, Updates, And Plans, Pamela Puppo, Katharine Gregg, Donna Ford-Werntz, James Vanderhorst, Michelle Mabry, Jeremy Keene

Biological Sciences Faculty Research

The West Virginia (U.S.A.) Herbarium Curators met on December 1st, 2021, with the intent of sharing updates on the collections, fostering collaboration, learning from each other's experiences, and identifying priorities for the collections towards the future. This article presents a summary of the herbaria that were represented in this meeting.

How Is Pelagic Sargassum-Associated Biodiversity Assessed? Insights From The Literature, Kristie S.T. Alleyne

Gulf and Caribbean Research

Over the past decade unprecedented blooming of pelagic Sargassum has occurred across the Equatorial Atlantic from West Africa to the Caribbean. Although pelagic Sargassum mats are considered beneficial in the open ocean, providing valuable habitat for a diverse array of endemic and associated species, they also inundate coastal areas and cause a plethora of management challenges for fisheries, tourism, nearshore coastal ecosystems, public health and the socioeconomic welfare of coastal communities. In—water harvesting has been suggested as a desirable management solution to prevent shoreline inundation, but destruction of the associated biodiversity is a concern with this approach and has not …

Digging For Information: Investigating The Efficacy Of Tracking And Augmenting Lacunicambarus Nebrascensis For Endangered Dragonfly Conservation, Danielle Elaine Tesar

Dissertations and Theses

Restoration and preservation of habitat for threatened and endangered species can proceed in many ways. Augmentation strategies can be used to supplement threatened and endangered populations or the species and resources on which those imperiled species depend. Comprehensive knowledge of species movement and home range is necessary to formulate effective augmentation plans. For freshwater burrowing crayfish, this type of information is generally lacking. The studies reported here were designed to acquire detailed information that is essential for augmentation of the plains devil crayfish, Lacunicambarus nebrascensis (formerly, Cambarus diogenes). Although L. nebrascensis populations are not considered threatened, this crayfish provides vital …

Patterns And Process Of Sandbar Revegetation On The Missouri National Recreational Rive, Amena Begum Ruma

Dissertations and Theses

Decades of flow regulation have reduced sandbar area and recruitment of cottonwood and willow along the Missouri River. Conflicts exist between managing sandbars for habitat (removing vegetation) for threatened sandbar-nesting birds (i.e., Piping Plover) and allowing natural recruitment of early successional riparian woodland (set-aside bars) that may support other species and ecological values. Recent changes in topography, geomorphology, and vegetation were examined on sandbars that have been “set aside” from management within seven reaches of the Missouri National Recreational River (MNRR) in southeastern South Dakota, USA. An existing time series of maps of sandbar landcover, derived from satellite imagery, was …

Exploring The Functionality Of Putative Bop3 Post-Translational Modifications, Liliya Tkachuk, Rebecca Adams Phd

[Archive] Belmont University Research Symposium (BURS)

All eukaryotic cells require that transcribed mRNAs undergo export form the nucleus to the cytoplasm where they can be translated into proteins. This process requires a host of proteins which are conserved between the unicellular budding yeast, S. cerevisiae, and humans. During this process, Mex67 and other associated proteins facilitate the mRNA to travel across the nuclear pore complex (NPC), doorways embedded in the nuclear envelope. Upon the exit of mRNA, Mex67 is released and recycled back into the nucleus to provide the export of more mRNA. This release occurs through the action of Dbp5, whose activity is regulated …

Isolation And Characterization Of Novel B. Subtilis Bacteriophages, Johnson Phung, Rebecca Adams

Science University Research Symposium (SURS)

Bacteriophages are viruses that infect bacteria cells. Bacteria, being the most abundant form of life on the planet, have an estimated ten to a hundred different types of bacteriophages that infect each species. Bacteriophages are everywhere on Earth, but the number of phages that have been identified is only a fraction of what are thought to exist. This project focuses on isolating and identifying phages that infect the bacterium Bacillus subtilis. B subtilis is a decomposition microbe commonly found in the soil. Our first goal was to determine whether the 3 samples of Bacillus subtilis phages that were previously …

Establishing A Biochemical System For The Purification And Atpase Activity Of Gst-Dbp5, Sarah R. Utley, Rachel E. Rigsby Phd, Rebecca L. Adams Phd

Science University Research Symposium (SURS)

The export of mRNA out of the nucleus is a crucial step for eukaryotic gene expression. The export of mRNA transcripts is aided by Mex67, which allows export through the nuclear pore complex doorways in the nuclear envelope. Once out of the nucleus, a protein known as Dbp5, bound to ATP, Gle1, and Nup42 aids in the directionality of mRNA export by helping remove Mex67 from the mRNA strand. Following interaction with RNA, Dbp5 then hydrolyzes ATP so that it unbinds the mRNA, allowing for enzyme recycling. Previous efforts worked towards the purification of Dbp5, but the attempts were unsuccessful …

Aerobic Vs. Anaerobic Exercise: The Role Of Exercise Attitudes, Frequency, And Perception Of Effort In Cognitive And Psychological Benefits, Khalil Dabdoub, Michael Oliver

Science University Research Symposium (SURS)

Exercise has been shown to have numerous benefits. Benefits often depend on the type and duration of exercise. Existing research focuses primarily on long-term aerobic exercise with studies showing 20-30 minutes of exercise to be beneficial. What remains unknown is how little exercise can show benefits. Anaerobic exercise has not been studied much, and when it has, findings have been mixed. Research is needed to elucidate the potential cognitive and psychological benefits of anaerobic exercise of different durations. In addition to exercise type, factors such as one’s attitude towards exercising, exercise frequency, and perception of effort may play a role …

Identifying Adaptor Proteins For Selective Mrna Export In S. Cerevisiae, Chloe Baker, Rebecca Adams

Science University Research Symposium (SURS)

The export of mature mRNA from the nucleus of the cell to the cytoplasm is crucial for a cell’s survival, as this process is necessary to produce proteins encoded by these transcripts. In S. cerevisiae (yeast) cells, a protein known as Mex67 must bind to the mRNA while it’s in the nucleus so it can cross through the nuclear pore complex (NPC). Mex67 cannot bind to the mRNA directly, instead it needs to be accompanied by adaptor proteins to aid it in getting across the NPC. Stress conditions such as heat shock interferes with the function of these adaptor …

Adenoviral-Vectored Centralized Consensus Hemagglutinin Vaccine Provides Broad Protection Against H2 Influenza A Virus, Erika M. Petro-Turnquist, Brianna L. Bullard, Matthew J. Pekarek, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

Several influenza pandemics have occurred in the past century, one of which emerged in 1957 from a zoonotic transmission of H2N2 from an avian reservoir into humans. This pandemic caused 2–4 million deaths and circulated until 1968. Since the disappearance of H2N2 from human populations, there has been waning immunity against H2, and this subtype is not currently incorporated into seasonal vaccines. However, H2 influenza remains a pandemic threat due to consistent circulation in avian reservoirs. Here, we describe a method of pandemic preparedness by creating an adenoviral-vectored centralized consensus vaccine design against human H2 influenza. We also assessed the …

What Is The Recorded Economic Cost Of Alien Invasive Fishes Worldwide?, Phillip J. Haubrock, Camille Bernery, Ross N. Cuthbert, Chunlong Liu, Melina Kourantidou, Boris Leroy, Anna J. Turbelin, Andrew M. Kramer, Laura Verbrugge, Christophe Diagne, Franck Courchamp, Rodolphe E. Gozlan

Integrative Biology Faculty and Staff Publications

Invasive alien fishes have caused pernicious ecological impacts on aquatic ecosystems. However, there has not been a global appraisal of associated economic impacts. Here, we compiled reported economic impacts of invasive alien fishes using the most comprehensive global database of invasion costs (InvaCost). We analyze how fish invasion costs are distributed geographically and temporally, as well as which socioeconomic sectors are most impacted. Fish invasions have caused the economic loss of at least US$32.8 billion globally (2017 value), from only 26 reported species (of 128 known invasive alien fish species). North America had the highest costs (> 99%), followed by …

Analysing Economic Costs Of Invasive Alien Species With The Invacost R Package, Boris Leroy, Andrew M. Kramer, Anne-Charlotte Vaissière, Melina Kourantidou, Franck Courchamp, Christophe Diagne

Integrative Biology Faculty and Staff Publications

1. The reported costs of invasive alien species from the global database InvaCost are heterogenous and cover different spatio-temporal scales. A standard procedure for aggregating invasive species cost estimates is necessary to ensure the repeatability and comparativeness of studies.

2. We introduce here the invacost R package, an open-source software designed to query and analyse the InvaCost database. We illustrate this package and its framework with cost data associated with invasive alien invertebrates.

3. First, the invacost package provides updates of this dynamic database directly in the analytical environment R. Second, it helps understand the heteregoneous nature of monetary cost data for invasive …

The Magnitude, Diversity, And Distribution Of The Economic Costs Of Invasive Terrestrial Invertebrates Worldwide, David Renault, Elena Angulo, Ross N. Cuthbert, Phillip J. Haubrock, César Capinha, Alok Bang, Andrew M. Kramer, Franck Courchamp

Integrative Biology Faculty and Staff Publications

Invasive alien species (IAS) are a major driver of global biodiversity loss, hampering conservation efforts and disrupting ecosystem functions and services. While accumulating evidence documented ecological impacts of IAS across major geographic regions, habitat types and taxonomic groups, appraisals for economic costs remained relatively sparse. This has hindered effective cost-benefit analyses that inform expenditure on management interventions to prevent, control, and eradicate IAS. Terrestrial invertebrates are a particularly pervasive and damaging group of invaders, with many species compromising primary economic sectors such as forestry, agriculture and health. The present study provides synthesised quantifications of economic costs caused by invasive terrestrial …

Biological Invasion Costs Reveal Insufficient Proactive Management Worldwide, Ross N. Cuthbert, Christophe Diagne, Emma J. Hudgins, Anna Turbelin, Danish A. Ahmed, Céline Albert, Thomas W. Bodey, Elizabeta Briski, Franz Essl, Phillip J. Haubrock, Rodolphe E. Gozlan, Natalia Kirichenko, Melina Kourantidou, Andrew M. Kramer, Franck Courchamp

Integrative Biology Faculty and Staff Publications

The global increase in biological invasions is placing growing pressure on the management of ecological and economic systems. However, the effectiveness of current management expenditure is difficult to assess due to a lack of standardised measurement across spatial, taxonomic and temporal scales. Furthermore, there is no quantification of the spending difference between pre-invasion (e.g. prevention) and post-invasion (e.g. control) stages, although preventative measures are considered to be the most cost-effective. Here, we use a comprehensive database of invasive alien species economic costs (InvaCost) to synthesise and model the global management costs of biological invasions, in order to provide a better …

Economic Costs Of Biological Invasions In The United States, Jean E. Fantle-Lepczyk, Phillip J. Haubrock, Andrew M. Kramer, Ross N. Cuthbert, Anna J. Turbelin, Robert Crystal-Ornelas, Christophe Diagne, Franck Courchamp

Integrative Biology Faculty and Staff Publications

The United States has thousands of invasive species, representing a sizable, but unknown burden to the national economy. Given the potential economic repercussions of invasive species, quantifying these costs is of paramount importance both for national economies and invasion management. Here, we used a novel global database of invasion costs (InvaCost) to quantify the overall costs of invasive species in the United States across spatiotemporal, taxonomic, and socioeconomic scales. From 1960 to 2020, reported invasion costs totaled $4.52 trillion (USD 2017). Considering only observed, highly reliable costs, this total cost reached $1.22 trillion with an average annual cost of …

Knowledge Gaps In Economic Costs Of Invasive Alien Fish Worldwide, Phillip J. Haubrock, Camille Bernery, Ross N. Cuthbert, Chunlong Liu, Melina Kourantidou, Boris Leroy, Anna J. Turbelin, Andrew M. Kramer, Laura N.H. Verbrugge, Christophe Diagne, Franck Courchamp, Rodolphe E. Gozlan

Integrative Biology Faculty and Staff Publications

Invasive alien fishes have had pernicious ecological and economic impacts on both aquatic ecosystems and human societies. However, a comprehensive and collective assessment of their monetary costs is still lacking. In this study, we collected and reviewed reported data on the economic impacts of invasive alien fishes using InvaCost, the most comprehensive global database of invasion costs. We analysed how total (i.e. both observed and potential/predicted) and observed (i.e. empirically incurred only) costs of fish invasions are distributed geographically and temporally and assessed which socioeconomic sectors are most affected. Fish invasions have potentially caused the economic loss of at least …

Computational Strain Analysis In Microbiome Datasets With Application To Skin Disease, Minerva Fatimae Ventolero

Electronic Theses and Dissertations, 2020-2023

Identifying bacterial strains is important not only for human health research but also for various environmental and industrial applications. The plethora of available shotgun metagenomic datasets provide an unprecedented opportunity to discover novel bacterial strains in different environmental niches. In this thesis, we evaluated the existing novel-strain-based tools and showed that their performance is still suboptimal. Due to the difficulty in distinguishing strains of similar abundance by available tools, the SMS (strains in multiple samples) tool was developed to de novo identify bacterial strains in multiple shotgun metagenomic samples. We showed that SMS distinguishes strains of similar abundance well and …

Wpa News 119 (2022), World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Newsletters

WPA News (Winter 2022), number 119

Published by the World Pheasant Association

Management Of The Brown Marmorated Stink Bug (Halyomorpha Halys) (Hemiptera:Pentatomidae) On Small Acreage And Organic Vegetable Systems, Lauren Fann

Theses and Dissertations--Entomology

Invasive species can be very disruptive to established integrated pest management programs. They can cause increases in broad-spectrum insecticide applications with variable effectiveness to mitigate damage. However, not all introduced species develop invasive characteristics; often, many introduced species go undetected. It is not until a species begins to cause economic damage to commodities essential to humans that it becomes classified as an invasive pest. Many challenges arise with the management of invasive pests due to lack of understanding of their biology, behavior, and absence of natural enemies in their introduced range. This often results in poor management and population outbreaks …

Blue Mussel Hybrid Complex Shifts In The Gulf Of Maine Towards A Dominance Of Mytilus Edulis, Daniel Woods

Honors Program Theses

The Gulf of Maine (GOM) in the United States is the fastest warming body of water in the world. Due to a complex history of glaciation, the GOM has restricted circulation that causes it to function more akin to an enclosed sea. As climate change has disrupted the stable flow of the Gulf Stream, more warm-water currents are entering the GOM at higher frequency. This warming has had a profound impact on the distribution of the blue mussel, Mytilus spp., which inhabits the rocky intertidal habitat within the GOM as the main prey species to a keystone predator. Two sister …

River Report. State Of The Lower St. Johns River Basin, Florida: Water Quality, Fisheries, Aquatic Life, Contaminants, 2022., Environmental Protection Board, City Of Jacksonville, University Of North Florida, Jacksonville University, Gerry Pinto, Gretchen Bielmyer-Fraser, John Burr, Dale Casamatta, Charles Closmann, Brian P. Zoellner, Ashley Johnson, An-Phong Le, William Penwell, Radha Pyati

State of the River Report

No abstract provided.

Dataset For: Mapping Fire History And Quantifying Burned Area Through 35 Years Of Prescribed Fire History At An Illinois Tallgrass Prairie Restoration Site Using Gis, Erin G. Rowland-Schaefer, Elizabeth M. Bach, Bill P. Kleiman, Holly P. Jones

Research Datasets

Fire was important to pre-colonization prairies. In today’s remnant and reconstructed prairies, managers frequently employ prescribed fire, a historical management practice that limits woody encroachment, suppresses non-native species, and promotes nutrient cycling. However, few long-term prescribed fire spatial datasets are available for study. We used archived images of prescribed fire maps and hand-drawn fire records to generate a geospatial record of the prescribed fire history at Nachusa Grasslands, a combination remnant and restored preserve in northern Illinois. This record contains maps of the prescribed fire history of the preserve across the entirety of its 35-year history. We used the maps …

Potential And Limitations Of Using Stem Cells To Cure Alzheimer’S Disease: A Literature Review Of Its Potential And Ethical Limitations In Translation To Human Trials, Eleni Zivla

OUR Journal: ODU Undergraduate Research Journal

Alzheimer's disease has become one of the most significant, life-limiting illnesses of our time as a result of the rapid increase in the average life expectancy. To successfully develop a cure for this yet incurable disease, one must understand the pathology of Alzheimer’s disease. As found in recent research studies, a brain that is diagnosed with Alzheimer's is characterized by the presence of extracellular amyloid plaques composed of the amyloid-beta (Aβ) peptide and intracellular neurofibrillary tangles composed of the microtubule-associated protein: tau. In this literature review, several stem cell therapies are being reviewed as a potential cure for Alzheimer’s disease …