Life Sciences Commons^™

Sorghum Bicolor - Environmental Weed Risk Assessment 2022, Department Of Primary Industries And Regional Development, Western Australia

Environmental Weed Risk Assessments

Introducing new plants to an area may have both positive and negative effects on the environment, economy and community. To minimise the negative environmental impact of introducing new agricultural species, DPIRD conducts a risk assessment procedure based on widely accepted scientific standards. This report assesses hybrid forage, grain annual sorghum, Sudan grass (Sorghum bicolor) Annual hybrid sorghum includes a wide range of forage and grain sorghum types (e.g. Sudan grass hybrids, Grain sorghum x Sudan hybrids, Sweet sorghum hybrids, open-pollinated sweet sorghum, dual-purpose grain sorghum hybrids). They are tall, leafy, erect, tussock grasses used as annual forage or hay crops, …

Trifolium Michelianum - Environmental Weed Risk Assessment 2022, Department Of Primary Industries And Regional Development, Western Australia

Environmental Weed Risk Assessments

Introducing new plants to an area may have both positive and negative effects on the environment, economy and community. To minimise the negative environmental impact of introducing new agricultural species, DPIRD conducts a risk assessment procedure based on widely accepted scientific standards. This report assesses Balansa clover (Trifolium michelianum). Balansa clover is a temperate annual pasture legume native to the Mediterranean region. It is sown in south-western Australia on soils subject to periodic waterlogging. In experimental trials in northern Western Australia (WA) the annual legumes and herbs had good feed quality but in general, with low forage yields under irrigation, …

Trifolium Repens - Environmental Weed Risk Assessment 2022, Department Of Primary Industries And Regional Development, Western Australia

Environmental Weed Risk Assessments

Introducing new plants to an area may have both positive and negative effects on the environment, economy and community. To minimise the negative environmental impact of introducing new agricultural species, DPIRD conducts a risk assessment procedure based on widely accepted scientific standards. This report assesses White clover (Trifolium repens). White clover is a herbaceous perennial legume that reproduces from seed as well as vegetatively from stolons. It is most likely native to Mediterranean Europe and has been used as a pasture legume in both Europe and the British Isles for centuries. It is widely sown throughout the world and has …

Trifolium Resupinatum - Environmental Weed Risk Assessment 2022, Department Of Primary Industries And Regional Development, Western Australia

Environmental Weed Risk Assessments

Introducing new plants to an area may have both positive and negative effects on the environment, economy and community. To minimise the negative environmental impact of introducing new agricultural species, DPIRD conducts a risk assessment procedure based on widely accepted scientific standards. This report assesses Persian clover (Trifolium resupinatum). Persian clover is an annual clover native to Turkey, Afghanistan, Portugal, Greece, Iran and Iraq. It was introduced to South Australia in the 1950s and grown commercially in the early 1970s. It has become a valuable species for temperate pastures of southern Australia, but is a minor pasture species in south-western …

Trifolium Vesiculosum - Environmental Weed Risk Assessment 2022, Department Of Primary Industries And Regional Development, Western Australia

Environmental Weed Risk Assessments

Introducing new plants to an area may have both positive and negative effects on the environment, economy and community. To minimise the negative environmental impact of introducing new agricultural species, DPIRD conducts a risk assessment procedure based on widely accepted scientific standards. This report assesses Arrowleaf clover (Trifolium vesiculosum). Arrowleaf clover (Trifolium vesiculosum) is an upright, annual pasture legume which occurs naturally in the Mediterranean Basin (Italy, Greece, Corsica, western Turkey, the Balkans and north of the Black Sea). Arrowleaf clover is a long season annual suited to forage production systems which grows strongly in spring but has poor winter …

Vitis Vinifera - Environmental Weed Risk Assessment 2022, Department Of Primary Industries And Regional Development, Western Australia

Environmental Weed Risk Assessments

Introducing new plants to an area may have both positive and negative effects on the environment, economy and community. To minimise the negative environmental impact of introducing new agricultural species, DPIRD conducts a risk assessment procedure based on widely accepted scientific standards. This report assesses Grapevines (Vitis vinifera). Grapevines are grown for both direct consumption of the fruit as table grapes or dried vine fruit and for fermentation to produce wine. They are cultivated worldwide, but originate from southern Europe. In Western Australia (WA) the viticulture industry was initially centred around the Swan Valley, but the work of Olmo (1956) …

Zea Mays - Environmental Weed Risk Assessment 2022, Department Of Primary Industries And Regional Development, Western Australia

Environmental Weed Risk Assessments

Introducing new plants to an area may have both positive and negative effects on the environment, economy and community. To minimise the negative environmental impact of introducing new agricultural species, DPIRD conducts a risk assessment procedure based on widely accepted scientific standards. This report assesses Maize (Zea mays). Maize (corn) is a tall, annual warm season (C4) grass grown for grain or silage. The first domestication of maize was by the indigenous people of southern Mexico, and it has been cultivated for thousands of years in central and southern America. Maize is a staple food in many parts of the …

Momentum, Rather Than Velocity, Is A More Effective Measure Of Improvements In Division Ia Football Player Performance, J. Bryan Mann, Jerry L. Mayhew, Marcel Lopes Dos Santos, J. Jay Dawes, Joseph F. Signorile

Faculty Publications - Kinesiology and Recreation

Speed, or the time to complete straight runs or agility drills, is commonly used to assess performance in collegiate American football players. However, it is common for players' speeds to plateau by the second year of eligibility, whereas their body masses continue to increase. The purpose of this study was to track change in speed, body mass, and momentum (body mass · velocity), across Division 1 football players' 4-year careers (n = 512). Complete data were derived for the 40-yd sprint (n = 82), the proagility shuttle (n = 73), and the L drill (n = 73) from the same …

Comparison Of Effects Of Sleep Fragmentation On Pro-Inflammatory Cytokine Il-1Β Of Male And Female Adult Mice, Manzar Rzayeva

Mahurin Honors College Capstone Experience/Thesis Projects

Sleep plays an essential role throughout the body by affecting the physiological function and regulation of many systems. One of these systems that receives effects as a result of the adequacy of sleep is the immune system. Previous studies have demonstrated effects of sleep fragmentation upon the immune system; however, sexual differences of these effects have not been studied in depth. To analyze these variances amongst the genders, male and female adult mice were subjected to acute sleep fragmentation (SF) for 24 hours in an automated SF cage that includes a bar sweeping across the cage every two minutes. Meanwhile, …

The Role Of Dichloro(Ethylenediamine) Platinum Ii In Altering Cellular Iron And Copper Transport, Megan Hall

Mahurin Honors College Capstone Experience/Thesis Projects

Approximately 50% of all chemotherapy cancer patients receive a platinum chemotherapeutic during their treatment. Although these drugs are widely utilized, little is known about the cellular transport of platinum drugs. It is hypothesized that platinum utilizes transporters for other metals to enter the cell. Structural differences between compounds are predicted to affect the compounds’ ability to exploit different transporters. Also, different cell types express different transporters. Therefore, compound structure is expected to influence transport of platinum compounds differently in different cell types. This study investigated the role of the platinum drug cisplatin and platinum compound Pt(en)Cl₂ in altering iron …

Crossmodal Perception Of Object Shape: A Study On The Effect Of Modal Order On Successful Shape Recognition, Ashlyn Vale

Mahurin Honors College Capstone Experience/Thesis Projects

Visual and haptic (tactile) modes of perception, while occasionally exercised independently, most often occur concurrently. The degree to which the ordering of the two modes of perception affects successful recognition of three-dimensional shapes varies. Some have found that the cross-modal orders (vision followed by haptics or vice versa) produce equal performance (Caviness, 1964; Lacey, Peters, & Sathian, 2007; Norman et al., 2006), while other researchers found visual-haptic (VH) performance to be superior to haptic-visual (HV) performance (Davidson, Abbott, & Gershenfeld, 1974; Norman, Clayton, Norman, & Crabtree, 2008). The current experiment used an old-new recognition task (with cookie cutter stimuli). In …

Root Phenotyping Of Peptide-Treated Glycine Max, Salem Jackson, Fiona Goggin

Inquiry: The University of Arkansas Undergraduate Research Journal

Plant elicitor peptides (Peps) – endogenous chains of amino acids involved in natural plant defense – have been shown to decrease damage from herbivores and pathogens by inducing an immune response, increasing the emission of volatile organic compounds (VOCS), transcripts, and metabolites. Exogenous treatment of soybean seeds with plant elicitor peptide GmPep3 has been shown to induce these broad-spectrum defenses and offers a new method for increasing crop yield. However, the effects of GmPep3 on indicators of soybean health – root characteristics, growth stages, etc. – have not been fully realized. Using the root-phenotyping platform RhizoVision Explorer, several root traits …

Investigating Replacement Of A Yeast Gene With A Human Gene As A Rare Disease Model System, Emma Pastoor, Amy Wilstermann, Rachael Baker

Summer Research

• BCS1L is a mitochondrial protein that assists in building complex III of the electron transport chain which allows for cellular respiration to occur
• Different mutations in BCS1L can result in three rare genetic diseases: Bjornstad Syndrome, BCS1L Mitopathy, or GRACILE Syndrome

Proteome Database, Mariana Rius, Jackie L. Collier, Joshua Rest

SoMAS Research Data

No abstract provided.

Differences In Stronger Versus Weaker Firefighters In Selected Measures Of Power, Quincy Johnson, J. Jay Dawes, Melissa Uftring, Marcel Lopes Dos Santos, Davis Hale, Gabriel J. Sanders, Will Peveler, Roger O. Kollock

Faculty Publications - Kinesiology and Recreation

Firefighters are required to perform a wide array of physically demanding job tasks, such as forcible entry, charged hose advances and victim extractions. An adequate level of muscular strength and power are required to successfully perform these tasks. The purpose of this study is to investigate the differences in stronger and weaker firefighters in measures of power. Archived data for twenty-seven (age = 34.3 ± 7.9 yr, body height = 176.3 ± 7.2 cm, body mass = 89.4 ± 15.7 kg) full-time firefighters were analyzed. Participants were placed into one of two groups [i.e., stronger (HIGH) (n = 13) …

An Artifact In Intracellular Cytokine Staining For Studying T Cell Responses And Its Alleviation., Zheng Gong, Qing Li, Jiayuan Shi, Guangwen Ren

Faculty Research 2022

Intracellular cytokine staining (ICS) is a widely employed ex vivo method for quantitative determination of the activation status of immune cells, most often applied to T cells. ICS test samples are commonly prepared from animal or human tissues as unpurified cell mixtures, and cell-specific cytokine signals are subsequently discriminated by gating strategies using flow cytometry. Here, we show that when ICS samples contain Ly6G⁺ neutrophils, neutrophils are ex vivo activated by an ICS reagent - phorbol myristate acetate (PMA) - which leads to hydrogen peroxide (H₂O₂) release and death of cytokine-expressing T cells. This artifact …

Genomic Heterogeneity Underlies Multidrug Resistance In Pseudomonas Aeruginosa: A Population-Level Analysis Beyond Susceptibility Testing., Laura J Rojas, Mohamad Yasmin, Jacquelynn Benjamino, Steven M Marshall, Kailynn J Deronde, Nikhil P Krishnan, Federico Perez, Andrew A Colin, Monica Cardenas, Octavio Martinez, Armando Pérez-Cardona, Daniel D Rhoads, Michael R Jacobs, John J Lipuma, Michael W Konstan, Alejandro J Vila, Andrea Smania, Andrew R Mack, Jacob G Scott, Mark D Adams, Lilian M Abbo, Robert A Bonomo

Faculty Research 2022

BACKGROUND: Pseudomonas aeruginosa is a persistent and difficult-to-treat pathogen in many patients, especially those with Cystic Fibrosis (CF). Herein, we describe a longitudinal analysis of a series of multidrug resistant (MDR) P. aeruginosa isolates recovered in a 17-month period, from a young female CF patient who underwent double lung transplantation. Our goal was to understand the genetic basis of the observed resistance phenotypes, establish the genomic population diversity, and define the nature of sequence evolution over time.

METHODS: Twenty-two sequential P. aeruginosa isolates were obtained within a 17-month period, before and after a double-lung transplant. At the end of the …

Genome-Wide Variant Calling In Reanalysis Of Exome Sequencing Data Uncovered A Pathogenic Tubb3 Variant., Elke De Boer, Burcu Yaldiz, Anne-Sophie Denommé-Pichon, Leslie Matalonga, Steve Laurie, Solve-Rd Snv-Indel Working Group, Daniel Danis, Peter N Robinson, Solve-Rd-Ditf Ithaca

Faculty Research 2022

Almost half of all individuals affected by intellectual disability (ID) remain undiagnosed. In the Solve-RD project, exome sequencing (ES) datasets from unresolved individuals with (syndromic) ID (n = 1,472 probands) are systematically reanalyzed, starting from raw sequencing files, followed by genome-wide variant calling and new data interpretation. This strategy led to the identification of a disease-causing de novo missense variant in TUBB3 in a girl with severe developmental delay, secondary microcephaly, brain imaging abnormalities, high hypermetropia, strabismus and short stature. Interestingly, the TUBB3 variant could only be identified through reanalysis of ES data using a genome-wide variant calling approach, despite …

Curation And Expansion Of Human Phenotype Ontology For Defined Groups Of Inborn Errors Of Immunity., Matthias Haimel, Julia Pazmandi, Raúl Jiménez Heredia, Jasmin Dmytrus, Sevgi Köstel Bal, Samaneh Zoghi, Paul Van Daele, Tracy A Briggs, Carine Wouters, Brigitte Bader-Meunier, Florence A Aeschlimann, Roberta Caorsi, Despina Eleftheriou, Esther Hoppenreijs, Elisabeth Salzer, Shahrzad Bakhtiar, Beata Derfalvi, Francesco Saettini, Maaike A A Kusters, Reem Elfeky, Johannes Trück, Jacques G Rivière, Mirjam Van Der Burg, Marco Gattorno, Markus G Seidel, Siobhan Burns, Klaus Warnatz, Fabian Hauck, Paul Brogan, Kimberly C Gilmour, Catharina Schuetz, Anna Simon, Christoph Bock, Sophie Hambleton, Esther De Vries, Peter N Robinson, Marielle Van Gijn, Kaan Boztug

Faculty Research 2022

BACKGROUND: Accurate, detailed, and standardized phenotypic descriptions are essential to support diagnostic interpretation of genetic variants and to discover new diseases. The Human Phenotype Ontology (HPO), extensively used in rare disease research, provides a rich collection of vocabulary with standardized phenotypic descriptions in a hierarchical structure. However, to date, the use of HPO has not yet been widely implemented in the field of inborn errors of immunity (IEIs), mainly due to a lack of comprehensive IEI-related terms.

OBJECTIVES: We sought to systematically review available terms in HPO for the depiction of IEIs, to expand HPO, yielding more comprehensive sets of …

Independent Dsg4 Frameshift Variants In Cats With Hair Shaft Dystrophy., Sarah Kiener, Ana Rostaher, Silvia Rüfenacht, Vidhya Jagannathan, John P Sundberg, Monika Welle, Tosso Leeb

Faculty Research 2022

Investigations of hereditary phenotypes in spontaneous mutants may help to better understand the physiological functions of the altered genes. We investigated two unrelated domestic shorthair cats with bulbous swellings of the hair shafts. The clinical, histopathological, and ultrastructural features were similar to those in mice with lanceolate hair phenotype caused by loss-of-function variants in Dsg4 encoding desmoglein 4. We sequenced the genomes from both affected cats and compared the data of each affected cat to 61 control genomes. A search for private homozygous variants in the DSG4 candidate gene revealed independent frameshift variants in each case, c.76del or p.Ile26fsLeu*4 in …

At-Home Blood Collection And Stabilization In High Temperature Climates Using, Lauren G Brown, Amanda J Haack, Dakota S Kennedy, Karen N Adams, Jennifer E Stolarczuk, Meg G Takezawa, Erwin Berthier, Sanitta Thongpang, Fang Yun Lim, Damien Chaussabel, Mathieu Garand, Ashleigh B Theberge

Faculty Research 2022

Expanding whole blood sample collection for transcriptome analysis beyond traditional phlebotomy clinics will open new frontiers for remote immune research and telemedicine. Determining the stability of RNA in blood samples exposed to high ambient temperatures (>30°C) is necessary for deploying home-sampling in settings with elevated temperatures (e.g., studying physiological response to natural disasters that occur in warm locations or in the summer). Recently, we have developed

The Th1/Tfh-Like Biased Responses Elicited By The Rasp-1 Innate Adjuvant Are Dependent On Trif And Type I Ifn Receptor Pathways., Parakkal Jovvian George, Radu Marches, Djamel Nehar-Belaid, Jacques Banchereau, Sara Lustigman

Faculty Research 2022

No abstract provided.

Single-Cell Transcriptome Analysis Defines Expression Of Kabuki Syndrome-Associated Kmt2d Targets And Interacting Partners., Badam Enkhmandakh, Paul Robson, Pujan Joshi, Anushree Vijaykumar, Dong-Guk Shin, Mina Mina, Dashzeveg Bayarsaihan

Faculty Research 2022

No abstract provided.

Targeting Sars-Cov-2 Infection Through Car-T-Like Bispecific T Cell Engagers Incorporating Ace2., Mikail Dogan, Lina Kozhaya, Lindsey Placek, Fatih Karabacak, Mesut Yigit, Derya Unutmaz

Faculty Research 2022

OBJECTIVES: Despite advances in antibody treatments and vaccines, COVID-19 caused by SARS-CoV-2 infection remains a major health problem resulting in excessive morbidity and mortality and the emergence of new variants has reduced the effectiveness of current vaccines.

METHODS: Here, as a proof-of-concept, we engineered primary CD8 T cells to express SARS-CoV-2 Spike protein-specific CARs, using the extracellular region of ACE2 and demonstrated their highly specific and potent cytotoxicity towards Spike-expressing target cells. To improve on this concept as a potential therapeutic, we developed a bispecific T cell engager combining ACE2 with an anti-CD3 scFv (ACE2-Bite) to target infected cells and …

Intergenomic And Epistatic Interactions Control Free Radical Mediated Pancreatic Β-Cell Damage., Jing Chen, Renhua Li, Sarah Knapp, Guizhi Zhu, Robert L Whitener, Edward H. Leiter, Clayton E Mathews

Faculty Research 2022

Alloxan (AL)-generated Reactive Oxygen Species (ROS) selectively destroy insulin-producing pancreatic β-cells. A previous genome-wide scan (GWS) using a cohort of 296 F2 hybrids between NOD (AL-sensitive) and ALR (AL-resistant) mice identified linkages contributing to β-cell susceptibility or resistance to AL-induced diabetes on Chromosomes (Chr) 2, 3, 8, and a single nucleotide polymorphism in

Engineering A "Detect And Destroy" Skin Probiotic To Combat Methicillin-Resistant Staphylococcus Aureus., Changhui Guan, Peter J Larson, Elizabeth Fleming, Alexander P Tikhonov, Sara Mootien, Trudy H Grossman, Caroline Golino, Julia Oh

Faculty Research 2022

The prevalence and virulence of pathogens such as methicillin-resistant Staphylococcus (S.) aureus (MRSA), which can cause recurrent skin infections, are of significant clinical concern. Prolonged antibiotic exposure to treat or decolonize S. aureus contributes to development of antibiotic resistance, as well as depletion of the microbiome, and its numerous beneficial functions. We hypothesized an engineered skin probiotic with the ability to selectively deliver antimicrobials only in the presence of the target organism could provide local bioremediation of pathogen colonization. We constructed a biosensing S. epidermidis capable of detecting the presence of S. aureus quorum sensing autoinducer peptide and producing lysostaphin …

Proceedings From The Albert Charitable Trust Inaugural Workshop On 'Understanding The Acute Effects Of Exercise On The Brain', Jill N. Barnes, Jeffrey M. Burns, Marcas M. Bamman, Sandra A. Billinger, Sue C. Bodine, Frank W. Booth, Patrice Brassard, Tameka A. Clemons, Paul J. Fadel, Paige C. Geiger, Swathi Gujral, Jacob M. Haus, Scott E. Kanoski, Benjamin F. Miller, Jill K. Morris, Kristen M S O'Connell, David C. Poole, Darleen A. Sandoval, J Carson Smith, Russell H. Swerdlow, Shawn N. Whitehead, Eric D. Vidoni, Henriette Van Praag

Faculty Research 2022

An inaugural workshop supported by "The Leo and Anne Albert Charitable Trust," was held October 4-7, 2019 in Scottsdale, Arizona, to focus on the effects of exercise on the brain and to discuss how physical activity may prevent or delay the onset of aging-related neurodegenerative conditions. The Scientific Program Committee (led by Dr. Jeff Burns) assembled translational, clinical, and basic scientists who research various aspects of the effects of exercise on the body and brain, with the overall goal of gaining a better understanding as to how to delay or prevent neurodegenerative diseases. In particular, research topics included the links …

Pdpn Marks A Subset Of Aggressive And Radiation-Resistant Glioblastoma Cells, Aram S. Modrek, Eskil Eskilsson, Ravesanker Ezhilarasan, Qianghu Wang, Lindsey D. Goodman, Yingwen Ding, Ze-Yan Zhang, Krishna P. Bhat, Thanh-Thuy T. Le, Floris P Barthel, Ming Tang, Jie Yang, Lihong Long, Joy Gumin, Frederick F. Lang, Roel G W Verhaak, Kenneth D. Aldape, Erik P. Sulman

Faculty Research 2022

Treatment-resistant glioma stem cells are thought to propagate and drive growth of malignant gliomas, but their markers and our ability to target them specifically are not well understood. We demonstrate that podoplanin (PDPN) expression is an independent prognostic marker in gliomas across multiple independent patient cohorts comprising both high- and low-grade gliomas. Knockdown of PDPN radiosensitized glioma cell lines and glioma-stem-like cells (GSCs). Clonogenic assays and xenograft experiments revealed that PDPN expression was associated with radiotherapy resistance and tumor aggressiveness. We further demonstrate that knockdown of PDPN in GSCs in vivo is sufficient to improve overall survival in an intracranial …

The Tsallis Generalized Entropy Enhances The Interpretation Of Transcriptomics Datasets, Nicolas Dérian, Hang-Phuong Pham, Djamel Nehar-Belaid, Nicolas Tchitchek, David Klatzmann, Vicaut Eric, Adrien Six

Faculty Research 2022

BACKGROUND: Identifying differentially expressed genes between experimental conditions is still the gold-standard approach to interpret transcriptomic profiles. Alternative approaches based on diversity measures have been proposed to complement the interpretation of such datasets but are only used marginally. METHODS: Here, we reinvestigated diversity measures, which are commonly used in ecology, to characterize mice pregnancy microenvironments based on a public transcriptome dataset. Mainly, we evaluated the Tsallis entropy function to explore the potential of a collection of diversity measures for capturing relevant molecular event information. RESULTS: We demonstrate that the Tsallis entropy function provides additional information compared to the traditional diversity …

Identification Of Distinct Immune Cell Subsets Associated With Asymptomatic Infection, Disease Severity, And Viral Persistence In Covid-19 Patients, Xiaorui Wang, Han Bai, Junpeng Ma, Hongyu Qin, Qiqi Zeng, Fang Hu, Tingting Jiang, Weikang Mao, Yang Zhao, Xiaobei Chen, Xin Qi, Mengyang Li, Jiao Xu, Jingcan Hao, Yankui Wang, Xi Ding, Yuanrui Liu, Tianlong Huang, Chao Fang, Changli Ge, Dong Li, Ke Hu, Xianwen Ren, Baojun Zhang, Binghong Zhang, Bingyin Shi, Chengsheng Zhang

Faculty Research 2022

The cell-mediated protective and pathogenic immune responses to SARS-CoV-2 infection remain largely elusive. Here we identified 76 distinct cell subsets in the PBMC samples that were associated with various clinical presentations of COVID-19 using scRNA-seq technology coupled with a deep and comprehensive analysis of unique cell surface markers and differentially expressed genes. We revealed that (TRAV1-2CD8)MAIT cells and (NCAM1CD160)NK cells significantly enriched in the asymptomatic subjects whereas (LAG3CD160CD8)NKT cells increased in the symptomatic patients. We also observed that (CD68CSF1RIL1BCD14)classical monocytes were positively correlated with the disease severity. Moreover, (CD33HLA-DMACD14)classical monocytes and (CLEC10AS100A9)pDC were associated with the viral persistence. The GO …