Life Sciences Commons^™

“It’S Up To Us:” Factors Influencing The Perspectives And Practices Of Instructors Working In An Out-Of-School Swimming Program For Underserved Children And Youth, Daniela M. Susnara, Matthew Curtner-Smith

International Journal of Aquatic Research and Education

The purposes of this study were (a) to describe the perspectives and practices of instructors working in an out-of-school swimming program (OSSP) for underserved children and youth and (b) to examine the factors which shaped these perspectives and practices. The theoretical framework employed was occupational socialization. Two female instructors participated in the study. Five qualitative techniques were used to collect data and standard interpretive techniques were employed to analyze them. Key findings were that the instructors were highly skilled and had well-developed sets of beliefs about teaching swimming and working with disadvantaged children and youth. These perspectives and practices were …

Effects Of A Demand-Valve Scuba Regulator On Cardiorespiratory Response During Submaximal Exercise Under Normobaric Conditions: A Preliminary Investigation, Christopher R. Kovacs Ph.D., Camille Dhom B.S.

International Journal of Aquatic Research and Education

SCUBA diving fatalities are often related to cardiac events triggered by stress linked to equipment. The purpose of this investigation was to examine the effects of a SCUBA regulator on cardiorespiratory exercise at a submaximal workload. Ten participants (mean = 21.5 yrs; s.d. = 1.16) completed two submaximal exercise tests at 1 ATA; one while breathing normally and a second while breathing directly from a demand valve SCUBA regulator. Total time to test completion (TOT), heart rate (HR), blood pressure (BP), rating of perceived exertion (RPE), and arterial oxygen levels were all assessed. No significant differences between conditions were found …

Reversible Lysine Fatty Acylation Of An Anchoring Protein Mediates Adipocyte Adrenergic Signaling., Rushita A Bagchi, Emma L Robinson, Tianjing Hu, Ji Cao, Jun Young Hong, Charles A Tharp, Hanan Qasim, Kathleen M Gavin, Julie Pires Da Silva, Jennifer L Major, Bradley K Mcconnell, Edward Seto, Hening Lin, Timothy A Mckinsey

Biochemistry and Molecular Medicine Faculty Publications

No abstract provided.

Examining Youth Conceptualizations Of Water Safety Behaviors Among Participants In A Learn-To-Swim Program, Austin R. Anderson, Kristina R. Anderson, William D. Ramos, Angela K. Beale-Tawfeeq

International Journal of Aquatic Research and Education

Drowning represents a leading cause of unintentional deaths among children. Concomitantly, while formal swim instruction often incorporates water safety practices, the efficacy of these messages remains largely unexplored. This qualitative-based study sought to evaluate how youth apply learned water safety concepts when posed with a hypothetical aquatic scenario. Semi-structured interviews, augmented by use of a visual illustration prompt, were conducted with 29 participants of a youth Learn-to-Swim (LTS) program. Interviews assessed participants’ expressed water safety behaviors and were coded based on emergent themes. Findings indicated that most participants were able to identify a “struggling” swimmer in the dramatized illustration. When …

Effect Of Water Depth On Heart Rate And Core Temperature During Underwater Treadmill Walking, Carrie E. Bajenski, Brianna R. Brandon, Cailey A. Curry, Leslie Fajardo, Ryan T. Conners

International Journal of Aquatic Research and Education

Exercising using an underwater treadmill (UTM) has become a popular modality; however, few studies have focused on the physiological demands of UTM walking at varying water depths. Thus, the objective of this study was to investigate changes in heart rate (HR) and core temperature (CT) values in college-aged males and females while exercising at different water immersion depths using an UTM. Twenty participants (age = 21.50 ± 2.19 years; height = 169.04 ± 10.85cm; weight = 75.56 ± 22.28kg) walked at water depths of 10cm below the xiphoid process and at the level of the superior iliac crest (I.C.). Each …

Using Ecological Niche Modeling To Predict The Response Of Hydrocotyle Bonariensis To Global Climate Change, Rachel Bonner, Laura K. Dease, Elizabeth A. Hughston

JSU Student Symposium 2022

Coastal dune habitats provide valuable ecosystem services but are declining globally due to the interacting effects of multiple stressors, including global climate change (GCC). To explore the potential severity of the effects of GCC on dune plant communities of the Northern Gulf of Mexico and surrounding areas, we modeled the fundamental bioclimatic niche of a dune plant species, largeleaf pennywort (Hydrocotyle bonariensis Comm. ex Lam.), in this region. We then projected its niche to estimate habitat suitability under current and future environmental conditions. Occurrence records were mined from the Global Biodiversity Information Facility, cleaned, then thinned by spatial filtering, …

Characterization Of Anti-Microbial Properties Of Excrement And Functional Microbiome Of New World Vultures In Alabama, Bridgette Gray

JSU Student Symposium 2022

Two distinct species of New World vultures are native to North America and inhabit Alabama, black vultures (Coragyps atratus) and turkey vultures (Cathartes aura). They are scavengers that consume decaying carcasses or carrion, which helps reduce the spread of disease. Vultures are susceptible to declining population numbers as they have been trapped and killed due to the belief that they spread disease, additionally contact with poisons and habitat destruction have also led to their decline. Currently, these two vultures are one of the seven vulture species with least concern and stable in Alabama and the world. However, it is important …

Diving Into The Unknown: A Genetic Investigation Of Type-2 Diabetes-Associated Insr Variants Of Uncertain Significance, Trinity Elston

JSU Student Symposium 2022

Type 2 diabetes is a complex metabolic disorder with interactions between genetic and environmental factors, making it more complicated to understand the clinical implications of identified genetic variants and disease pathology. Many of the diabetes-associated variants are currently classified as VUS and occur within the genes involved in insulin signaling and regulation, so we turned our focus on the human INSR gene. INSR encodes the insulin receptor protein that plays a major role in insulin signaling. Furthermore, C. elegans provides a simple model system to examine the functional consequences of these diabetes-associated VUS. We examined INSR diabetes-associated VUS through the …

Butterfly Eyespots Evolved Via Cooption Of An Ancestral Gene-Regulatory Network That Also Patterns Antennae, Legs, And Wings, Suriya Narayanan Murugesan, Heidi Connahs, Yuji Matsuoka, Mainak Das Gupta, Galen J. Tiong, Manizah Huq, Thomas Werner, Et. Al.

Michigan Tech Publications, Part 1

Butterfly eyespots are beautiful novel traits with an unknown developmental origin. Here we show that eyespots likely originated via cooption of parts of an ancestral appendage gene-regulatory network (GRN) to novel locations on the wing. Using comparative transcriptome analysis, we show that eyespots cluster most closely with antennae, relative to multiple other tissues. Furthermore, three genes essential for eyespot development, (), (), and (), share similar regulatory connections as those observed in the antennal GRN. CRISPR knockout of -regulatory elements (CREs) for and led to the loss of eyespots, antennae, legs, and also wings, demonstrating that these CREs are highly …

Biogeosciences Perspectives On Integrated, Coordinated, Open, Networked (Icon) Science, D. Dwivedi, A. L. D. Santos, M. A. Barnard, T. M. Crimmins, A. Malhotra, K. A. Rod, K. S. Aho, S. M. Bell, B. Bomfim, F. Q. Brearley, H. Cadillo-Quiroz, J. Chen, C. M. Gough, E. B. Graham, C. R. Hakkenberg, L. Haygood, G. Koren, E. A. Lilleskov, L. K. Meredith, S. Naeher, Z. L. Nickerson, O. Pourret, H.-S. Song, M. Stahl, N. Taş, R. Vargas, S. Weintraub-Leff

Department of Food Science and Technology: Faculty Publications

This article is composed of three independent commentaries about the state of Integrated, Coordinated, Open, Networked (ICON) principles in the American Geophysical Union Biogeosciences section, and discussion on the opportunities and challenges of adopting them. Each commentary focuses on a different topic: (a) Global collaboration, technology transfer, and application (Section 2), (b) Community engagement, community science, education, and stakeholder involvement (Section 3), and (c) Field, experimental, remote sensing, and real-time data research and application (Section 4). We discuss needs and strategies for implementing ICON and outline short- and long-term goals. The inclusion of global data and international …

A Novel Non‑Selective Atypical Pkc Agonist Could Protect Neuronal Cell Line From A Β ‑Oligomer Induced Toxicity By Suppressing A Β Generation, Dongmei Zou, Qian Li, Wenyang Pan, Peng Chen, Miao Sun, Xiaofeng Bao

Publications and Research

Atypical protein kinase C (aPKCs) serve key functions in embryonic development by regulating apical-basal polarity. Previous studies have shed light on their roles during adulthood, especially in the development of Alzheimer's disease (AD). Although the crystal structure of PKCι has been resolved, an agonist of aPKCs remains to be discovered. In the present study, by using the Discovery Studio program and LibDock methodology, a small molecule library (K66-X4436 KINA Set) of compounds were screened for potential binding to PKCι. Subsequently, the computational docking results were validated using affinity selection-mass spectrometry, before in vitro kinase activity was used to determine the …

Escherichia Coli Alanyl-Trna Synthetase Maintains Proofreading Activity And Translational Accuracy Under Oxidative Stress, Arundhati Kavoor, Paul Kelly, Michael Ibba

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Aminoacyl-tRNA synthetases (aaRSs) are enzymes that synthesize aminoacyl-tRNAs to facilitate translation of the genetic code. Quality control by aaRS proofreading and other mechanisms maintains translational accuracy, which promotes cellular viability. Systematic disruption of proofreading, as recently demonstrated for alanyl-tRNA synthetase (AlaRS), leads to dysregulation of the proteome and reduced viability. Recent studies showed that environmental challenges such as exposure to reactive oxygen species can also alter aaRS synthetic and proofreading functions, prompting us to investigate if oxidation might positively or negatively affect AlaRS activity. We found that while oxidation leads to modification of several residues in Escherichia coli AlaRS, unlike …

Fusarium And Verticillium Wilts Of Vegetables, Nick Volesky, Marion Murray, Claudia Nischwitz

All Current Publications

Fusarium wilt and Verticillium wilt are soilborne plant diseases that attack vascular tissue. They cause similar symptoms in their hosts and are difficult to manage as they can survive in the soil for many years. Once a plant is infected, there is no treatment or cure. Therefore, management should focus on preventive and cultural control practices. This fact sheet identifies the many vegetables susceptible to these pathogens in Utah and reviews symptoms, diagnosis, and ways to prevent these diseases.

How To Promote Intuitive Eating In Adolescents, Madeline Peterson, Stacy Bevan, Mateja R. Savoie-Roskos, Brooke Parker

All Current Publications

Intuitive eating is a pattern of eating that includes awareness of hunger and fullness cues and encourages an appropriate and respectful response to them. It is a non-diet approach to eating and is not focused on weight loss or weight gain—although changes in weight can occur for individuals who adopt or practice intuitive eating. This fact sheet discusses how to promote intuitive eating in adolescents as a way to build a healthier relationship with food and improve body image.

Investigation Of Eating Disorder Risk And Body Image Dissatisfaction Among Female Competitive Cheerleaders, Allison B. Smith, Jennifer L. Gay, Eva V. Monsma, Shawn M. Arent, Mark A. Sarzynski, Dawn M. Emerson, Toni M. Torres-Mcgehee

Faculty Publications

Social agents associated with cheerleading environments are increasingly linked to body image dissatisfaction (BID) and eating disorders (ED). This study examined ED risk across team type, squad type, and position. An additional purpose determined BID in clothing type (daily clothing, midriff uniform, and full uniform), and meta-perceptions from the perspective of peers (MP peers), parents (MP parents), and coaches (MP coaches). Female cheerleaders ( = 268) completed an online survey which included demographics, the Eating Attitudes Test-26, and pathogenic behavior questions. Body image perceptions were assessed by using the Sex-Specific Figural Stimuli Silhouettes. Overall, 34.4% of cheerleaders ( = 268; …

Investigation Of Eating Disorder Risk And Body Image Dissatisfaction Among Female Competitive Cheerleaders, Allison B. Smith, Jennifer L. Gay, Eva V. Monsma, Shawn M. Arent Phd, Cscs*D, Fissn, Facsm, Fnak, Mark A. Sarzynski Ph.D., Faha, Facsm, Dawn M. Emerson Ph.D., Lat, Atc, Toni Torres-Mcgehee Phd, Lat, Atc

Faculty Publications

Social agents associated with cheerleading environments are increasingly linked to body image dissatisfaction (BID) and eating disorders (ED). This study examined ED risk across team type, squad type, and position. An additional purpose determined BID in clothing type (daily clothing, midriff uniform, and full uniform), and meta-perceptions from the perspective of peers (MP peers), parents (MP parents), and coaches (MP coaches). Female cheerleaders (n = 268) completed an online survey which included demographics, the Eating Attitudes Test-26, and pathogenic behavior questions. Body image perceptions were assessed by using the Sex-Specific Figural Stimuli Silhouettes. Overall, 34.4% of cheerleaders (n …

Your Next Celebration At Us National Parks: A Case Emphasizing Lyme Neuroborreliosis (Bannwarth Syndrome), Ganesh Arun, Farhan Ali, Sowmya Srinivas, Harsha Surath, Justin Nistico, Dayakar Reddy

Advances in Clinical Medical Research and Healthcare Delivery

Introduction:

Bannwarth syndrome is synonymous with Lyme neuroborreliosis. The neurological component consists of neuropathy, radicular pain, and lymphocytic pleocytosis. This is due to inflammation of the nerve fibers by Borrelia Burgdorferi. Borrelia Borgdorferi is a spirochete bacteria transmitted hematogenously from the Ixodes tick vector to the human host. The commonly known Lyme disease is diagnosed with symptoms of arthritis, heart block, and integumentary signs. Lyme disease can present as early disseminated or late manifestations of disease. Bannwarth syndrome takes it a step further and emphasizes the neurologic sub set of symptoms. The Centers for Disease Control and Prevention (CDC) estimates …

Silage Preparation And Fermentation Characteristics Of Pearl Millet Stover Treated With Microbial Additive In West Africa, Y. Cai, Z. Du, S. Yamasaki, D. Jethro, M. Nignan

IGC Proceedings (1977-2023)

Pearl millet (Pennisetum glaucum L.) stover is one of the main crop by-products in West Africa. To effectively use the crop by-product resources as roughage for ruminant to cover the feed shortage in the dry season, the millet stover silage was prepared using laboratory-scale fermentation system without (control) or with lactic acid bacteria inoculant (LP, Lactobacillus plantarum) and cellulase enzyme (AC, Acremonium) in Burkina Faso, and their fermentation characteristics and chemical composition were analyzed. The dry matter (DM) of fresh millet before ensiling was 44.3%, and their organic matter, crude protein (CP), ether extract, neutral detergent fiber …

Seasonal Patterns Of Herbage Accumulation Dynamics In Marandu Palisadegrass Subjected To Intensities Of Continuous Stocking Management, André F. Sbrissia, Daniel Schmitt, Paulo G. Duchini, Sila C. Da Silva

IGC Proceedings (1977-2023)

It is relatively well reported in the literature that pastures can have similar forage net accumulation when managed with contrasting structures. However, we hypothesized that the patterns of forage accumulation dynamics of pastures managed at different canopy heights is dependent on environmental conditions. The experimental treatments were four canopy heights (10, 20, 30, and 40 cm), allocated to experimental units according to a randomized complete block design with four replicates and evaluated throughout four contrasting environmental seasons (Summer, Autumn, Winter-Early Spring, and Late Spring). Under favourable growing conditions greater forage accumulation was observed in pastures maintained taller; on the contrary, …

Screening Of Napier Grass (Pennisetum Purpureum Schumac.) Clones For Stunt Disease In Western Kenya, Caroline A. O. Kute

IGC Proceedings (1977-2023)

Napier stunt disease (NSD) is a threat to Napier grass farming in the smallholder dairy industry in east and central Africa. NSD in Kenya is caused by Candidatus Phytoplasma oryzae (Ns-phytoplasma) belonging to the 16SrXI group; vectored by a leaf hopper Maiestas banda. The objective of the study was to determine the incidence (proportion of diseased leaves) and severity (proportion of leaf area diseased [PLAD]) of stunt disease in Napier clones by screening in Bungoma, Kakamega and Busia Counties in Western Kenya. The Napier clones were germplasm collections maintained at KALRO Kitale and Muguga nurseries and in farmers’ fields. …

Revisiting Nitrogen Fertilisation Rates Of Kikuyu And Kikuyu-Ryegrass Pastures, C. Viljoen, Janke Van Der Colf, Pieter A. Swanepoel

IGC Proceedings (1977-2023)

Irrigated pastures are used for dairy production in South Africa. Minimum-tillage and nitrogen (N) fertilisation are important management practices for kikuyu (Pennisetum clandestinum) and ryegrass (Lolium spp.) pastures. Nitrogen fertiliser application rates as high as 500 kg N ha^-1 year^-1 have been reported. Conventional tillage as well as cutting and removal of herbage material (opposed to removal through grazing) are the basis on which these fertiliser guidelines were developed. The current management practices have substantially changed the soil organic carbon and N stoichiometry. The aim of this study was to determine an optimum rate of …

Review Of Urochloa Breeder’S Toolbox With The Theory Of Change And Stage Gate System Approach, E. Sweitzer, L. Hernandez, D. Florian, A. Notenbaert, S. Burkart, J. Arango, J. Cardoso, M. Peters, V. Castiblanco

IGC Proceedings (1977-2023)

Livestock production in the global south is at crossroads as there is a demand to increase Animal Source Foods to address hunger and pressure to lighten the environmental footprint often associated with livestock production. To satisfy both needs, the use of technologies that improve animal performance, while reducing land use and net Greenhouse Gas emissions produced by animals is essential. One of such technologies are Urochloa forage grasses.

Urochloa forage grasses are well known for their rusticity and their ability to grow in soils of low fertility and high aluminum content. These characteristics allow Urochloa to grow in areas temporally …

Residual Feed Intake (Rfi) And Efficiency Of Feed Utilization (Efu) Of Total Mixed Ration (Tmr) Fed Frisian Cross Bred Cows – A Case Study, S. C. Somasiri, A. M. C. S. Karunarathna

IGC Proceedings (1977-2023)

Residual Feed Intake (RFI) is the difference between an animal’s actual feed and expected feed intakes for a given period of time. Literature shows that highly efficient animals have a negative or lower RFI. Thus understanding about RFI is important to maintain an efficient herd. A feeding trial was undertaken at a commercial farm in Sri Lanka having Frisian cross bred cows. The cows were fed with a grass-based Total Mixed Ration (TMR) having maize (Zea mays), CO3 (Pennisetum perpureum X Pennisetum americarnum), beer pulp (wet brewer’s grain), dhal meal (Lens culinaris), mineral mixture …

Ua12/2/1 Up Next On The Hill, Vol. 97, No. 6, Wku Student Affairs

WKU Administration Documents

Magazine edition of the College Heights Herald for the period Feb. 14 to March 21, 2022.

• Collins, Michael. I Went to School There? University Plan Marks New Era of Campus for Next Generation of Students
• Trickett-Wile, Arthur & Jake Jones. Like Farmer, Like Son – Alex Burke
• Stover, D.J. Gun Violence Hasn’t Left, from a Survivor of Marshall County
• Lanuzza, Izzy. International Bonds Key to WKU Tennis Success

The Low Abundance Of Cpg In The Sars-Cov-2 Genome Is Not An Evolutionarily Signature Of Zap, Ali Afrasiabi, Hamid Alinejad-Rokny, Azad Khosh, Mostafa Rahnama, Nigel Lovell, Zhenming Xu, Diako Ebrahimi

Plant Pathology Faculty Publications

The zinc finger antiviral protein (ZAP) is known to restrict viral replication by binding to the CpG rich regions of viral RNA, and subsequently inducing viral RNA degradation. This enzyme has recently been shown to be capable of restricting SARS-CoV-2. These data have led to the hypothesis that the low abundance of CpG in the SARS-CoV-2 genome is due to an evolutionary pressure exerted by the host ZAP. To investigate this hypothesis, we performed a detailed analysis of many coronavirus sequences and ZAP RNA binding preference data. Our analyses showed neither evidence for an evolutionary pressure acting specifically on CpG …

The Astounding World Of Glycans From Giant Viruses, Anna Notaro, Chantal Abergel, Rosa Lanzetta, Todd L. Lowary, Antonio Molinaro, Michela Tonetti, James L. Van Etten, Cristina De Castro

Department of Plant Pathology: Faculty Publications

Viruses are a heterogeneous ensemble of entities, all sharing the need for a suitable host to replicate. They are extremely diverse, varying in morphology, size, nature, and complexity of their genomic content. Typically, viruses use host-encoded glycosyltransferases and glycosidases to add and remove sugar residues from their glycoproteins. Thus, the structure of the glycans on the viral proteins have, to date, typically been considered to mimick those of the host. However, the more recently discovered large and giant viruses differ from this paradigm. At least some of these viruses code for an (almost) autonomous glycosylation pathway. These viral genes include …

What Kigali’S Open‑Air Markets Reveal About Achieving Food And Nutrition Security: The Role Of African Indigenous Crops, Eugene Baraka, Mary S. Willis, Brice A. Ishimwe

Department of Nutrition and Health Sciences: Faculty Publications

Background: Household dietary diversity in Rwanda remains low and significantly contributes to the double burden of malnutrition. Rwanda has one of the highest under five stunting rates globally, and malnutrition remains one of the most pressing public health issues; therefore, factors that shape food and nutrition security are of utmost concern. Globally, the variety of foods available in open-air markets has been shown to affect dietary diversity. Furthermore, the consumption of indigenous foods can contribute to a diverse diet and improve nutrition status. At present, there are limited data on foods available for purchase in open-air markets in Africa. …

The Association Between Rest-Activity Rhythms And Glycemic Markers: The Us National Health And Nutrition Examination Survey, 2011-2014

Faculty, Staff and Student Publications

OBJECTIVES: Previous studies conducted in mostly homogeneous sociodemographic samples have reported a relationship between weakened and/or disrupted rest-activity patterns and metabolic dysfunction. This study aims to examine rest-activity rhythm characteristics in relation to glycemic markers in a large nationally representative and diverse sample of American adults.

METHODS: This study used data from the National Health and Nutrition Examination Survey 2011-2014. Rest-activity characteristics were derived from extended cosine models using 24-hour actigraphy. We used multinomial logistic regression and multiple linear regression models to assess the associations with multiple glycemic markers (i.e., glycated hemoglobin, fasting glucose and insulin, homeostatic model assessment of …

Fungal Mycobiome Drives Il-33 Secretion And Type 2 Immunity In Pancreatic Cancer, Aftab Alam, Eric Levanduski, Parker Denz, Helena Solleiro Villavicencio, Maulasri Bhatta, Yali Zhang, Brian Morreale, Sharon Senchanthisai, Jun Li, Steven G Turowski, Sandra Sexton, Sheila Jani Sait, Prashant K Singh, Jianmin Wang, Anirban Maitra, Pawel Kalinski, Ronald A Depinho, Huamin Wang, Wenting Liao, Scott I Abrams, Brahm H Segal, Prasenjit Dey

Faculty, Staff and Student Publications

TH2 cells and innate lymphoid cells 2 (ILC2) can stimulate tumor growth by secreting pro-tumorigenic cytokines such as interleukin-4 (IL-4), IL-5, and IL-13. However, the mechanisms by which type 2 immune cells traffic to the tumor microenvironment are unknown. Here, we show that oncogenic KrasG12D increases IL-33 expression in pancreatic ductal adenocarcinoma (PDAC) cells, which recruits and activates TH2 and ILC2 cells. Correspondingly, cancer-cell-specific deletion of IL-33 reduces TH2 and ILC2 recruitment and promotes tumor regression. Unexpectedly, IL-33 secretion is dependent on the intratumoral fungal mycobiome. Genetic deletion of IL-33 or anti-fungal treatment decreases TH2 and ILC2 infiltration and increases …

Hybrid Seed Set In Relation With Male Floral Traits, Estimation Of Heterosis And Combining Abilities For Yield And Its Components In Wheat (Triticum Aestivum L.), Samira El Hanafi, Souad Cherkaoui, Zakaria Kehel, Miguel Sanchez-Garcia, Jean-Benoit Sarazin, Stephen Baenziger, Wuletaw Tadesse

Department of Agronomy and Horticulture: Faculty Publications

Breeding hybrids with maximum heterosis requires efficient cross-pollination and an improved male sterility system. Renewed efforts have been made to dissect the phenotypic variation and genetic basis of hybrid floral traits, although the potential of tailoring the appropriate flower design on seed setting is less known. To this end, elite wheat genotypes were crossed using a chemical hybridizing agent at different doses. A total of 23 hybrids were developed from a partial diallel design; and planted in an alpha lattice design with their parents at two locations in Morocco, for two years, to evaluate for yield components, heterosis and combining …