Life Sciences Commons^™

Eeg As Successful Diagnostic Tool For Immune Effector Cell Associated Neurotoxicity Syndrome, Daniel Jones, Christine Eckhardt, Haoqi Sun, Ryan Tesh, Brandon Westover

Library/Life Sciences Undergraduate Poster Competition 2022

CAR T cell therapy was first proven effective in 2013¹ for the treatment of certain cancer s . However, its widespread adoption is still hindered by side effects like immune effector cell associated neurotoxicity syndrome (ICANS). Current research aims to better predict, diagnose, and treat ICANS so that CAR T cell therapy can be more widely applied. Although prior studies have attempted to identify an objective biomarker of ICANS² , current practice relies on clinical assessment to diagnose ICANS. We hypothesize that qualitative features of the EEG may be utilized to develop a physiological grading system of the …

Interruption Of Junctional Adhesion Molecules Shows Developmental Defects, Wesley Allen, Nathan Beckett, Lauren Hall, Emma Holdaway, Sion Jung, Meredith Mann, Spencer Thacker, Jacob Wengler, Cailey Winn, Dario Mizrachi, Michael Stark

Library/Life Sciences Undergraduate Poster Competition 2022

Development of the central nervous system, the brain and the spinal cord, starts initially with the formation of the neural tube. This vital process starts as a sheet of cells which then folds into a cylindrical structure in the early stages of embryo development (Fig1). Defects in the formation of the neural tube can lead to permanent disabilities in babies after birth. This is important to understand as current statistics show that approximately 300,000 babies are born annually with neural tube defects. (NTDs). In addition to NTDs there are many other dangerous birth defects that are important to understand as …

Introducing Xanthoria Parietina: Invasive, Naturalized, Or Opportunistic?, Mikele Baugh, Steve Leavitt

Library/Life Sciences Undergraduate Poster Competition 2022

Lichens are one of the world’s ubiquitous group of organisms. They have been documented from frigid tundras to arid deserts. While some species are known to be particularly hardy and flexible in their habitats, many species are linked to specific growing conditions. As the global climate changes, the geographical range for different growing conditions shifts. Economic trade and human travel provide new opportunities for species to access new locations that are beginning to develop habitable conditions. Xanthoria parietina has been observed in the Great Basin (a region outside of its typical coastal distribution) found in newly built horticultural landscapes. Individuals …

The Casnr L.I.N.K.S. Newsletter: Learning Innovation Network For K-12 Schools, Edition 3, March 2022, Bailey Feit, Tammy Mittelstet

CASNR L.I.N.K.S. Newsletter

The purpose of this L.I.N.K.S. newsletter is to provide a monthly update of opportunities and resources for K-12 schools to connect with the University of Nebraska's CASNR Food, Energy, Water, and Societal Systems (FEWS²) programs and partners.

Included in this edition:

Classroom resources for all levels

Eye on curriculum and resources

Teacher opportunities

List of new partnerships

Ask an expert

Eye on CASNR research: Trenton Franz

Send your students to campus

Success stories

Salicylic Acid And N-Hydroxypipecolic Acid At The Fulcrum Of The Plant Immunity-Growth Equilibrium, Alyssa Shields, Vanessa Shivnauth, Christian Danve M. Castroverde

Biology Faculty Publications

Salicylic acid (SA) and N-hydroxypipecolic acid (NHP) are two central plant immune signals involved in both resistance at local sites of pathogen infection (basal resistance) and at distal uninfected sites after primary infection (systemic acquired resistance). Major discoveries and advances have led to deeper understanding of their biosynthesis and signaling during plant defense responses. In addition to their well-defined roles in immunity, recent research is emerging on their direct mechanistic impacts on plant growth and development. In this review, we will first provide an overview of how SA and NHP regulate local and systemic immune responses in plants. We …

Survey Of Visual Opsin Evolution Across Caddisflies (Insecta: Trichoptera), Ashlynn Powell, Jacqueline Heckenhauer, Steffen Pauls, Paul Frandsen

Library/Life Sciences Undergraduate Poster Competition 2022

Caddisflies are a highly diverse order of aquatic insects. As eggs, larvae, and pupae, they occupy freshwater habitats, while as adults, they are generally aerial and terrestrial¹. Such varied environments call for a flexible and complex visual system. Visual systems are controlled by light-sensing molecules called opsins, which are categorized by the wavelength of light they are most sensitive to². In insects, these categories are:

• Long wavelength (LW)
• Short wavelength (SW)
• Ultraviolet wavelength (UV)

Adaptive duplication of opsin genes is the primary mechanism of evolution that allows for greater visual capacity³. While opsins have …

Overcoming Detrimental Potato Fungi In Potato Fields With The Secondary Metabolites Of Naturally Occurring Steptomyces, Hansan Jones, Jacob Anderson, Erik Kemp, Brad Geary, Matthew Madsen, Ryan Stewart

Library/Life Sciences Undergraduate Poster Competition 2022

• Fungal disease Silver scurf can cause 13% water loss and give poor aesthetic appeal, both of which reduce farmers profits (1,2).
• Pythium leak can result in up to 50% total loss of potatoes in storage (3)
• Streptomyces is the right candidate to mitigate or eliminate these losses because of its ability to survive in soil (4) and its history as a proven biocontrol for other plant pathogens (5).

The Mrna/Protein Relationship Is Fluid And Changes Across Tissue And Diseased States, Jose Humberto Giraldez Chavez, Sam Payne

Library/Life Sciences Undergraduate Poster Competition 2022

• Cancer is a dysregulated cell
• Molecular typing via DNA/RNA/protein
• Is RNA a proxy for cell?

Staying Hydrated: A Comparative Analysis Of Humectants In Human Skin, Ayden Olsen, Rachel Prince, Craig Reeves, Cassie Peterson, Jason Adams

Library/Life Sciences Undergraduate Poster Competition 2022

Humectants are an important class of compounds that attract and retain water within a cell. When mixed with water to create wetting solutions, humectants can prevent desiccation of cadaveric specimens. Recognizing a relative scarcity of comparative studies analyzing the effects of various wetting solutions on post preservation cadaveric maintenance, we utilized wet-dry analysis in order to compare the effects of four common humectants on water retention in human skin tissue. We created a concentration gradient for each humectant to identify the optimal concentrations of each compound for water retention, after which we compared water retention in tissue at the optimal …

Does Bathing After Sexual Assault Or Time Elapsed Have A Greater Effect On The Development Of Codis Eligible Dna Profiles?, Emily Black, Sam Payne, Julie Valentine, Leslie Miles

Library/Life Sciences Undergraduate Poster Competition 2022

Which has a greater effect on the development of CODIS eligible DNA profiles from SAKs – bathing/showering status of the victim or time between the assault and examination?

Comparison Of Tardigrade Extraction Methods, Abigail Andros

Library/Life Sciences Undergraduate Poster Competition 2022

It is a time-consuming process to pick tardigrades for an experiment. The standard method is to add water to the sample, then look through it and pick out tardigrades with a micropipette or small wire loop (Tsujimoto et al. 2015).

Improving The Germination Rate Of Gambel Oak (Quercus Gambelii) For Mineland Restoration, Ethan Ostraff, Christopher Miller, April Hulet, Bradley Geary, Kate Ruebelmann, Matthew Madsen

Library/Life Sciences Undergraduate Poster Competition 2022

Quercus gambelii Nutt. (Gambel oak) is an ecologically important species that provides food and shelter for many wildlife species. In areas impacted by disturbance, Q. gambelii is often the first woody perennial species to reestablish (USFS, 2016). The use of this species could be beneficial for the reclamation of mine sites in mountain shrubland areas of the western United States. However, due to the recalcitrant nature of these acorns (i.e. seeds that do not survive after drying or freezing), viability can quickly be lost after the acorns are harvested. Additionally, invasive weevils (Family Curculionidae) infest and kill oak acorns. These …

The Metabolic Effects Of Exercise And A Pre-Exercise Snack During A 30-Hour Fast, Katelynn Hales, Hil'ilei Chan, Landon S. Deru, Jake Moss, Bruce W. Bailey Ph.D.

Library/Life Sciences Undergraduate Poster Competition 2022

As incidence rates of chronic illnesses continue to increase, many pharmacological and lifestyle interventions have been undertaken to pinpoint effective treatment and prevention tools to combat them. One such intervention of recent interest includes the use of fasting. Ketones, a product of fat breakdown, are thought to be a key player in some of the health benefits achieved through fasting. Despite potential benefits, adhering to a fasting regimen can be difficult. While it is generally well-tolerated, some experience negative side effects,² indicating potential issues with long-term adherence. Heilbronn et al. suggest that a small meal on fasting days may be …

A Chromosome-Scale Assembly Of The Djulis (Chenopodium Formosanum) Genome, An Emerging Orphan Crop From Taiwan, Peter J. Maughan, Eric N. Jellen, John Sproul, Karol Krak, Tzu-Yun Huang, Tzu Che Lin, Beatriz Navarro Dominguez, Yung-Fen Huang, Kate Jaggi, David E. Jarvis

Library/Life Sciences Undergraduate Poster Competition 2022

Djulis (Chenopodium formosanum Koidz.) is a semi-domesticated Taiwanese native crop. It is a BCD-genome hexaploid (2n = 6x = 54) relative of the Andean-native allotetraploid (AABB) quinoa (C. quinoa). Djulis has been grown in a relatively small area by aboriginal Taiwanese, who use it primarily as a fermentation starter to produce ritual alcoholic beverages. Djulis’ seed protein, like that of quinoa, is complete in terms of its human dietary essential amino acid profile; the seed also offers a wide variety of nutritionally important vitamins and minerals. These nutritional attributes have attracted international attention as a novel, healthy grain option. We …

Whole-Body Vibration Alleviates Symptoms Of Morphine Withdrawal, Gavin C. Jones, Christina A. Small, Dallin Z. Otteson, Scott C. Steffensen

Library/Life Sciences Undergraduate Poster Competition 2022

Whole body vibration at 80 Hz has been shown to blunt neuropathological markers and behavioral symptoms of alcohol dependence Here, we evaluate its ability to ameliorate symptoms of morphine use and withdrawal.

The Role Of Cd5 In Cd4+ T Cell Metabolism, Joshua Bennett, Kiara Whitley, Claudia Freitas Phd, Christopher Haynie, Carlos Moreno, Scott Weber

Library/Life Sciences Undergraduate Poster Competition 2022

T cells serve a key role in the immunological response in the adaptive system. Different receptors present on the T cell have certain roles and are able to either inhibit or stimulate signaling which consequently regulates the function and metabolism of the cell. CD5 is an inhibitory co receptor expressed on the surface of T cells known to regulate thymocyte selection and TCR signaling . Our goal is to better understand the effects of CD5 regulation in metabolism. Using metabolic flux assays, we found that CD5KO naïve T cells have increased glycolysis and mitochondrial respiration in comparison to wild type …

Reviewing The Effects Of Human-Induced Limb Damage In Crabs, Jacob B. Spencer, Blaine D. Griffen

Library/Life Sciences Undergraduate Poster Competition 2022

Limb damage in crabs comes in various forms, each with unique consequences. Brachyuran crabs shed limbs as a defensive reflex, with natural breakage occurring along a plane, otherwise known as autotomy. In addition to autotomy, crabs commonly lose limbs due to fishing practices such as declawing. In many areas, taking the limbs and releasing the crab is a common practice. The logic is that the populations won’t decline if the crab is put back. Other practices such as nicking, or cutting the main claw tendon, are used to restrict claw movement. Various effects of these fishery practices have been documented …

Validity Of Ultrasound Imaging For Intrinsic Foot Muscle Cross-Sectional Area Measurements Demonstrated By Strong Agreement With Mri, Dallin C. Swanson, Joshua K. Sponbeck, Derek A. Swanson, Connor D. Stephens, Steven P. Allen, Ulrike H. Mitchell, James D. George, A. Wayne Johnson

Library/Life Sciences Undergraduate Poster Competition 2022

Intrinsic foot muscles maintain foot structural integrity and contribute to functional movement, posture and balance Thus, assessing intrinsic foot muscle size and strength are important One way to assess strength of a muscle is by measuring its cross sectional area ( Magnetic resonance imaging ( and ultrasound ( are two modalities allowing for visualization of these muscle bodies MRI has been shown to accurately image the individual muscles but is costly and time consuming US imaging may provide an alternative that is less costly and more readily available The purpose of this study was to investigate the validity and reproducibility …

Overcoming Detrimental Potato Fungi With The Secondary Metabolites Of Naturally Occurring Streptomyces In A Greenhouse, Hansan Jones, Jacob Anderson, Erik Kemp, Brad Geary, Matthew Madsen, Ryan Stewart

Library/Life Sciences Undergraduate Poster Competition 2022

• Fungal disease Silver scurf can cause 13% water loss and give poor aesthetic appeal, both of which reduce farmers profits (1,2).
• Pythium leak can result in up to 50% total loss of potatoes in storage (3).
• Streptomyces is the right candidate to mitigate or eliminate these losses because of its ability to survive in soil (4) and its history as a proven biocontrol for other plant pathogens (5).

Morphological Changes Of The Asian Shore Crab Across Latitudes, Ainslee Mcmullin, Blaine D. Griffen

Library/Life Sciences Undergraduate Poster Competition 2022

The Asian shore crab, Hemigrapsus sanguineus , is an invasive species that has rapidly spread across the Eastern United States coastline. First observed in North America in 1988, its range currently extends from Maine to North Carolina. H. sanguineus has adapted very well to this region and has displaced several native species as the dominant crab in rocky intertidal habitats. Although the Asian shore crab’s biology and interactions with native species has been well studied, larger scale impacts (economic, potential further spread, community ecology, etc.) are under researched. We collected specimen samples of H. sanguineus throughout its entire East coast …

Double Up Food Bucks Csa Program, Hannah Martin, Kelsey Hall

Applied Sciences, Technology and Education Faculty Publications

Double Up Food Bucks (Double Up) is a statewide produce incentive program that allows Supplemental Nutrition Assistance Program (SNAP) customers to access more locally growth, fresh vegetables and fruits at farmers markets, farm stands, and community supported agriculture (CSA) programs. A program of the Utah Department of Health, the benefits of Double Up and SNAP are four-fold: (1) families with low income eat more healthy food, (2) local farmers gain new customers and make more money, (3) more food dollars stay in the local economy, and (4) your community gains increased awareness of your CSA for participation in the Double …

Double Up Food Bucks Farm Stand Program, Hannah Martin, Kelsey Hall

Applied Sciences, Technology and Education Faculty Publications

Double Up Food Bucks (Double Up) is a statewide produce incentive program that allows Supplemental Nutrition Assistance Program (SNAP), formally known as food stamps program, customers to access more locally grown, fresh vegetables and fruits at farmers markets, farm stands, and community supported agriculture (CSA) programs. A program of the Utah Department of Health, the benefits of Double Up and SNAP are three-fold: families with low income eat more healthy food, local farmers gain new customers and make more money, and more food dollars stay in the local economy.

Evaluation Of A Hands-On Cooking Class And Its Effects On Self-Efficacy In Relation To Healthy Eating In Type 2 Diabetics, Codi A. Jenshak-Gorzinski

Doctor of Nursing Practice Scholarly Projects

The prevalence of Type 2 diabetes is alarming and poses a great threat to U.S. national health. Chronic diabetes can lead to serious conditions such as heart attack and stroke, and can result in premature mortality (World Health Organization, 2018). Diabetes can be prevented or treated by eating a healthy diet. Cooking classes are a potential way to improve American’s knowledge of nutritional principles and help them become confident that they have the capacity to eat a healthy diet. The purpose of this DNP project was to determine if participation in cooking classes enhanced self-efficacy as it pertains to healthy …

Nebline, March/April 2022

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Become a Groundwater Steward

4 Ways to Celebrate National Nutrition Month

Recipe of the Month

Selecting Fruit Trees for Eastern Nebraska

Garden Guide: Things to Do This Month

2022 Chemigation Trainings

Lancaster County Ag VIP Awards

Upcoming Early Childhood Trainings

Aleyna Cuttlers Participated in National 4-H Congress

4-H Horse Judging Team at Nationals

Meet 2021–22 4-H Council

Meet 2021–22 4-H Teen Council

Hear of 4-H Volunteer Award: Sherry Kubicek

Hear of 4-H Volunteer Award: Angie Root

4th & 5th Grade Fun Night, March 4

Rabbit Show, March 19

UNL Chick Days, March 26

Bucket Calf Workshop, March 27

Dressage Schooling …

Living A Long Life Is A Multi-Step Process, Alexander H. K. Montoye, Scott A. Conger

Kinesiology Faculty Publications and Presentations

There is a strong link between physical activity and health. Historically, large-scale epidemiological studies have used self-report surveys to capture physical activity measures (eg, type and intensity). In the past 20–30 years, large cohort studies have increasingly adopted devices, such as pedometers, accelerometers, and consumer-marketed activity trackers, to measure physical activity. Device-based physical activity measures alleviate some limitations of self-reporting by increasing measurement objectivity and accuracy. Additionally, device-based measures often show stronger associations with health outcomes than do self-reported measures, showcasing their value in understanding how physical activity affects health. Unlike self-report, device-based measures can capture steps, which are easily …

Multiple Genome Viewer (Mgv): A New Tool For Visualization And Comparison Of Multiple Annotated Genomes., Joel E Richardson, Richard M. Baldarelli, Carol J Bult

Faculty Research 2022

The assembled and annotated genomes for 16 inbred mouse strains (Lilue et al., Nat Genet 50:1574-1583, 2018) and two wild-derived strains (CAROLI/EiJ and PAHARI/EiJ) (Thybert et al., Genome Res 28:448-459, 2018) are valuable resources for mouse genetics and comparative genomics. We developed the multiple genome viewer (MGV; http://www.informatics.jax.org/mgv ) to support visualization, exploration, and comparison of genome annotations within and across these genomes. MGV displays chromosomal regions of user-selected genomes as horizontal tracks. Equivalent features across the genome tracks are highlighted using vertical 'swim lane' connectors. Navigation across the genomes is synchronized as a researcher uses the scroll and zoom …

The Mutant Mouse Resource And Research Center (Mmrrc): The Nih-Supported National Public Repository And Distribution Archive Of Mutant Mouse Models In The Usa., James Amos-Landgraf, Craig Franklin, Virginia Godfrey, Franziska Grieder, Kristin Grimsrud, Ian Korf, Cathleen Lutz, Terry Magnuson, Oleg Mirochnitchenko, Samit Patel, Laura G Reinholdt, K C Kent Lloyd

Faculty Research 2022

The Mutant Mouse Resource and Research Center (MMRRC) Program is the pre-eminent public national mutant mouse repository and distribution archive in the USA, serving as a national resource of mutant mice available to the global scientific community for biomedical research. Established more than two decades ago with grants from the National Institutes of Health (NIH), the MMRRC Program supports a Consortium of regionally distributed and dedicated vivaria, laboratories, and offices (Centers) and an Informatics Coordination and Service Center (ICSC) at three academic teaching and research universities and one non-profit genetic research institution. The MMRRC Program accepts the submission of unique, …

Systematic Reconstruction Of Cellular Trajectories Across Mouse Embryogenesis., Chengxiang Qiu, Junyue Cao, Beth K Martin, Tony Li, Ian C Welsh, Sanjay Srivatsan, Xingfan Huang, Diego Calderon, William Stafford Noble, Christine M Disteche, Stephen A Murray, Malte Spielmann, Cecilia B Moens, Cole Trapnell, Jay Shendure

Faculty Research 2022

Mammalian embryogenesis is characterized by rapid cellular proliferation and diversification. Within a few weeks, a single-cell zygote gives rise to millions of cells expressing a panoply of molecular programs. Although intensively studied, a comprehensive delineation of the major cellular trajectories that comprise mammalian development in vivo remains elusive. Here, we set out to integrate several single-cell RNA-sequencing (scRNA-seq) datasets that collectively span mouse gastrulation and organogenesis, supplemented with new profiling of ~150,000 nuclei from approximately embryonic day 8.5 (E8.5) embryos staged in one-somite increments. Overall, we define cell states at each of 19 successive stages spanning E3.5 to E13.5 and …

Annotated Expression And Activity Data For Murine Recombinase Alleles And Transgenes: The Creportal Resource., Michelle N Perry, Constance M. Smith, Hiroaki Onda, Martin Ringwald, Stephen A Murray, Cynthia L Smith

Faculty Research 2022

Recombinase alleles and transgenes can be used to facilitate spatio-temporal specificity of gene disruption or transgene expression. However, the versatility of this in vivo recombination system relies on having detailed and accurate characterization of recombinase expression and activity to enable selection of the appropriate allele or transgene. The CrePortal ( http://www.informatics.jax.org/home/recombinase ) leverages the informatics infrastructure of Mouse Genome Informatics to integrate data from the scientific literature, direct data submissions from the scientific community at-large, and from major projects developing new recombinase lines and characterizing recombinase expression and specificity patterns. Searching the CrePortal by recombinase activity or specific recombinase gene …

Mouse Genome Informatics (Mgi): Latest News From Mgd And Gxd., Martin Ringwald, Joel E Richardson, Richard M. Baldarelli, Judith A. Blake, James A. Kadin, Cynthia Smith, Carol J Bult

Faculty Research 2022

The Mouse Genome Informatics (MGI) database system combines multiple expertly curated community data resources into a shared knowledge management ecosystem united by common metadata annotation standards. MGI's mission is to facilitate the use of the mouse as an experimental model for understanding the genetic and genomic basis of human health and disease. MGI is the authoritative source for mouse gene, allele, and strain nomenclature and is the primary source of mouse phenotype annotations, functional annotations, developmental gene expression information, and annotations of mouse models with human diseases. MGI maintains mouse anatomy and phenotype ontologies and contributes to the development of …