Life Sciences Commons^™

Comparison Of Transcervical Insemination Versus Surgical Insemination Methods In Dogs At A Veterinary Clinic In Lincoln, Nebraska, Lauren Ahlers

Honors Program: Senior Projects (Public)

Artificial insemination paved the way for many advancements in reproductive technology. Two common artificial insemination methods recommended for use with frozen semen are transcervical insemination and surgical insemination. To better understand the conception rates of dogs for each method, data was collected from Hillcrest Animal Clinic. The data suggested that neither transcervical insemination nor surgical insemination was significantly better than the other. 50% of the dogs conceived when inseminated using the transcervical method, and 54.5% of the dogs conceived when inseminated using the surgical method. Based on the results, more data needs to be collected on both insemination procedures to …

Academic Information Seeking Behaviour Of Undergraduate Students Of History And International Studies In Nigerian Federal Universities, Chidi Amaechi Ph.D, Joseph Oluchukwu Wogu Ph.D

Library Philosophy and Practice (e-journal)

The study aims to understand the academic information-seeking behaviour of undergraduate students of history and international studies in Nigerian federal universities. A descriptive survey research design was adopted for the study. The study respondents were South-East federal universities undergraduate students of 100-400 levels in South-East, Nigeria's history and international studies department. The study was borne out of the numerous behavioural patterns exhibited by undergraduate students, history, and international studies undergraduate students inclusive for them to excel in their academic pursuits. 110 undergraduate students of history and international studies were sampled for the study. In determining the sample size, a random …

Measurable Residual Disease Response And Prognosis In Treatment-Naïve Acute Myeloid Leukemia With Venetoclax And Azacitidine, Keith W Pratz, Brian A Jonas, Vinod Pullarkat, Christian Recher, Andre C Schuh, Michael J Thirman, Jacqueline S Garcia, Courtney D Dinardo, Vladimir Vorobyev, Nicola S Fracchiolla, Su-Peng Yeh, Jun Ho Jang, Muhit Ozcan, Kazuhito Yamamoto, Arpad Illes, Ying Zhou, Monique Dail, Brenda Chyla, Jalaja Potluri, Hartmut Döhner

Faculty, Staff and Student Publications

PURPOSE: There is limited evidence on the clinical utility of monitoring measurable residual disease (MRD) in patients with acute myeloid leukemia treated with lower-intensity therapy. Herein, we explored the outcomes of patients treated with venetoclax and azacitidine who achieved composite complete remission (CRc; complete remission + complete remission with incomplete hematologic recovery) and MRD < 10

METHODS: The patients included in this report were treated with venetoclax and azacitidine. Bone marrow aspirate samples for multiparametric flow cytometry assessments were collected for central analysis at baseline, end of cycle 1, and every three cycles thereafter. MRD-negative response was defined as < 1 residual blast per 1,000 leukocytes (< 10

RESULTS: One hundred …

Blood-Based Biomarker Panel For Personalized Lung Cancer Risk Assessment, Johannes F Fahrmann, Tracey Marsh, Ehsan Irajizad, Nikul Patel, Eunice Murage, Jody Vykoukal, Jennifer B Dennison, Kim-Anh Do, Edwin Ostrin, Margaret R Spitz, Stephen Lam, Sanjay Shete, Rafael Meza, Martin C Tammemägi, Ziding Feng, Samir M Hanash

Faculty, Staff and Student Publications

Purpose: To investigate whether a panel of circulating protein biomarkers would improve risk assessment for lung cancer screening in combination with a risk model on the basis of participant characteristics.

Methods: A blinded validation study was performed using prostate lung colorectal ovarian (PLCO) Cancer Screening Trial data and biospecimens to evaluate the performance of a four-marker protein panel (4MP) consisting of the precursor form of surfactant protein B, cancer antigen 125, carcinoembryonic antigen, and cytokeratin-19 fragment in combination with a lung cancer risk prediction model (PLCOm2012) compared with current US Preventive Services Task Force (USPSTF) screening criteria. The 4MP was …

Understanding Common Perceptions Of The Drylands, C. Bedelian, D. Pertaub, F. Ngarachu, C. Hesse, S. Krätli, J. Obando, C. Shisanya, J. Maraigua

IGC Proceedings (1977-2023)

Drylands occupy over 40% of the earth’s surface and are home to more than 2 billion people. Drylands are key to the food and nutritional security of the planet and vital to the economies and livelihoods of dryland inhabitants. Yet, drylands are commonly perceived as unproductive, economically inefficient and marginal lands where small-scale farmers and pastoralists practice environmentally degrading activities. Driven by inadequate understanding and misconceptions of the drylands, policy-makers devise inappropriate policies and interventions. The BRECcIA project developed an online Drylands Perceptions Survey to understand the perceptions of researchers, practitioners and policy makers working in the drylands of Kenya, …

Relationships Between Biodiversity And Production In Grasslands At Local And Regional Scales, A. Hector, M. Loreau

IGC Proceedings (1977-2023)

Key points

1. Experimental manipulations of plant species diversity in unfertilised prairies and meadows has revealed that increasing diversity often leads to increased productivity (range of observed relationships varies from flat to log-linearly positive); driven by a combination of facilitation, niche-partitioning and sampling/selection effects.

2. The longer-term effects of diversity on ecosystem stability are not as clear and in need of further work.

3. Recent applied work, and a new review of the grassland literature, both show the potential for biodiversity to increase productivity under realistic field conditions.

4. The longer-term feedback of grazers on biodiversity gradients is unknown, and …

Methane And Nitrous Oxide Emissions From Grazed Grasslands, Harry Clark, C. Pinares-Patiño, C. De Klein

IGC Proceedings (1977-2023)

Key points

1. Emissions of methane (CH₄) and nitrous oxide (N₂O) from grasslands make a substantial contribution to total agricultural emissions of these two gases.

2. At present practical mitigation options that relate to grazing ruminants and grazed pastures are limited.

3. Research into agricultural greenhouse gas emissions is of low priority in most developed countries.

4. Direct manipulation of the rumen ecosystem provides the best opportunity for large reductions in CH₄ in the long term.

5. Reducing the amount of nitrogen (N) excreted by grazing animals is a priority in N₂O research, …

Mesobuthus Zarudnyi Sp. N. From Azerbaijan (Scorpiones: Buthidae), Nizami E. Novruzov, František Kovařík, Victor Fet

Euscorpius

A new species Mesobuthus zarudnyi sp. n. is described from Azerbaijan, fully illustrated with color photos. We compare the new species with two other species, M. eupeus (C. L. Koch, 1839) and M. persicus (Pocock, 1899), both found also in Azerbaijan.

Phenotypic Evaluation Of Energycane Varieties For Bioenergy, Wyatt Eason, D. M. Hollowell, Anna Hale, Jesse Ira Morrison, Brian Baldwin

IGC Proceedings (1977-2023)

The need for renewable and carbon-neutral energy is growing as fossil fuel supplies decrease and concerns of climate change increase. C₄ grasses are among the most efficient carbon accumulators. Sugarcane (Saccharum officinarum) is a renewable bioenergy crop with low inputs and high yields, but it is limited to tropical and sub-tropical climates. A close relative, Saccharum spontaneum, readily hybridizes with S. officinarum. S. spontaneum lends cold tolerance and higher yields to the hybrid progeny, called energycane. The Sugarcane Breeding Unit (USDA, Houma, Louisiana, USA) selected twenty-six genotypes for testing at Mississippi State University to determine production …

Effect Of Winter Canola Cultivar On Seed Yield, Oil, And Protein Content, Kyle D. Mcgeary, Jason P. De Koff, Bharat Pokharel, Richard Link, Priya Saini, Taqdeer Gill

Agricultural and Environmental Sciences Faculty Research

Winter canola (Brassica napus L.) production has increased in the United States over the past several decades; however, there is little research in the southeastern United States on its agronomic production and growth characteristics under different nitrogen (N) fertilizer rates. The objectives of this study were to determine the effects of N rates on winter mortality, yield, oil and protein content, and seedpod shatter resistance across cultivars. Canola was grown in a randomized split complete block design with four replicates under five different N rates (0, 56, 112, 168, and 224 kg N ha−1) with four different cultivars (Hekip, Inspiration, …

Smokejumper Obituary: Wiltzen, Harris Anthony (Missoula 1948), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Bumble Bees Exhibit Body Size Clines Across An Urban Gradient Despite Low Genetic Differentiation, Matthew W. Austin, Amber D. Tripodi, James P. Strange, Aimee S. Dunlap

Educator Preparation & Leadership Faculty Works

Environmental heterogeneity resulting from human-modified landscapes can increase intraspecific trait variation. However, less known is whether such phenotypic variation is driven by plastic or adaptive responses to local environments. Here, we study five bumble bee (Apidae: Bombus) species across an urban gradient in the greater Saint Louis, Missouri region in the North American Midwest and ask: (1) Can urban environments induce intraspecific spatial structuring of body size, an ecologically consequential functional trait? And, if so, (2) is this body size structure the result of plasticity or adaptation? We additionally estimate genetic diversity, inbreeding, and colony density of these species—three …

Climate Effects On Black Spruce And Trembling Aspen Productivity In Natural Origin Mixed Stands, Mahadev Sharma

Aspen Bibliography

Forest managers need site productivity estimates for tree species growing in mixed stands. Models developed in the past are generally for pure stands and don’t factor in the effects of climate change on site productivity. Therefore, site index (SI) models were developed for black spruce (Picea mariana Mill. B.S.P.) and trembling aspen (Populus tremuloides Michx.) trees grown in natural origin mixed stands. For this, 186 trees (93 black spruce and trembling aspen each) were sampled from 31 even-aged natural mixed stands (sites) (3 trees/species/site) across Ontario, Canada. Stand height growth models were developed by incorporating climate variables during …

Drainage Law Proposal, J. David Aiken

Cornhusker Economics

Legislative Bill 806 would significantly change Nebraska drainage law and bring long-overdue reform to Nebraska water law. This newsletter gives a quick overview of LB 806 and how it would impact the resolution of drainage disputes in Nebraska.

Quantitative Assessment Of The Trophic Ecology Of The Oceanic Ctenophore, Bolinopsis Infundibulum, In Monterey Bay, California, Victoria C. Scriven

USF Tampa Graduate Theses and Dissertations

Bolinopsis infundibulum is a wide-ranging, ubiquitous ctenophore whose fragile nature makes the collection of specimens and quantification of key predator-prey activities in controlled laboratory experiments, challenging. Thus, in situ methods often represent the best means for data collection. However, while present in surface waters, these animals can also be abundant at depths well beyond those attainable by divers. As a result, very little empirical data exist over the depth range of their natural habitats which limits our ability to assess key predator-prey interactions needed to assess their ecological role in midwater food webs. Working in Monterey Bay, California, remotely operated …

Elucidating Mechanisms Of Genetic Cross-Disease Associations At The Procr Vascular Disease Locus, David Stacey, Lingyan Chen, Paulina J Stanczyk, Joanna M M Howson, Amy M Mason, Stephen Burgess, Stephen Macdonald, Jonathan Langdown, Harriett Mckinney, Kate Downes, Neda Farahi, James E Peters, Saonli Basu, James S Pankow, Weihong Tang, Nathan Pankratz, Maria Sabater-Lleal, Paul S De Vries, Nicholas L Smith, Amy D Gelinas, Daniel J Schneider, Nebojsa Janjic, Nilesh J Samani, Shu Ye, Charlotte Summers, Edwin R Chilvers, John Danesh, Dirk S Paul

Faculty, Staff and Student Publications

Many individual genetic risk loci have been associated with multiple common human diseases. However, the molecular basis of this pleiotropy often remains unclear. We present an integrative approach to reveal the molecular mechanism underlying the PROCR locus, associated with lower coronary artery disease (CAD) risk but higher venous thromboembolism (VTE) risk. We identify PROCR-p.Ser219Gly as the likely causal variant at the locus and protein C as a causal factor. Using genetic analyses, human recall-by-genotype and in vitro experimentation, we demonstrate that PROCR-219Gly increases plasma levels of (activated) protein C through endothelial protein C receptor (EPCR) ectodomain shedding in endothelial cells, …

Impact Of New Technologies On Weed Control In Louisiana Rice Production, David C. Walker

LSU Doctoral Dissertations

Field studies were conducted in 2018-2021 at the LSU Agricultural Center’s H.R. Caffey Rice Research Station (RRS) near Crowley, LA, and the Dean Lee Research and Extension Center (DLREC) near Alexandria, LA to evaluate off-target florpyrauxifen movement on soybean. Soybean was treated with florpyrauxifen at the V4-V5 or R1-R2 growth stages. Soybean injury was evaluated at 1, 7, 14, and 28 days after treatment (DAT) and soybean plant height (cm) was recorded at 7, 14, and 28 DAT. At soybean maturity, yield and individual yield components were subjected to regression analysis to describe the relationship between florpyrauxifen rate and soybean …

Optimal Iron Content In Ready-To-Use Therapeutic Foods For The Treatment Of Severe Acute Malnutrition In The Community Settings: A Protocol For The Systematic Review And Meta-Analysis, Aamer Imdad, Melissa François, Fanny F. Chen, Abigail Smith, Olivia Tsistinas, Emily Tanner Smith, Jai K. Das, Zulfiqar Ahmed Bhutta

Department of Paediatrics and Child Health

Introduction: The current standard of care for children with severe acute malnutrition (SAM) involves using ready-to-use therapeutic food (RUTF) to promote growth; however, the precise formulation to achieve optimal recovery remains unclear. Emerging research suggests that alternative RUTF formulations may be more effective in correcting SAM-related complications such as anaemia and iron deficiency. This systematic review commissioned by the WHO aims to synthesise the most recent research on the iron content in RUTF and related products in the community-based treatment of uncomplicated severe malnutrition in children aged 6 months and older.
Methods and analysis: We will search multiple electronic databases. …

Grazing Land Contributions To Carbon Sequestration, Ronald F. Follett, G. E. Schuman

IGC Proceedings (1977-2023)

Key points

1. Grazing management can be used to increase soil organic carbon sequestration.

2. Grazing land soils contain large amounts of carbon with depth, and can store it for centuries.

3. Policies to encourage terrestrial carbon sequestration through conservation and good management of grazing lands are critical for many countries and the world.

Global Atmospheric Change And Its Effect On Managed Grassland Systems, Andreas Lüscher, J. Fuhrer, P. C. D. Newton

IGC Proceedings (1977-2023)

Key points

1. Increasing atmospheric CO2 concentration and a trend to warmer mean temperatures are the most reliable aspects of global atmospheric change. Projections of the extent of climate change and the frequency of extreme weather conditions remain uncertain.

2. Research has considerably reduced the uncertainty about effects of global atmospheric change on physiology of plants, productivity and species composition of plant communities.

3. Other factors (e.g. nutrient availability, soil type) and long-term adaptation of the ecosystem (e.g. nutrient cycling and sequestration) influence the response of plant communities to global atmospheric change. Generalisation is not possible with respect to the …

Dpo: Diffuse Pulmonary Ossification - A Diagnostic Challenge, Gaetano Rea, Giuliana Giacobbe, Danila Caroppo, Silvia Iovine, Roberta Lieto, Marialuisa Bocchino, Tullio Valente, Angelantonio Maglio, Alessandro Vatrella

Translational Medicine @ UniSa

Diffuse pulmonary ossification (DPO) is a rare condition of DLD (diffuse lung disease) characterized by the presence of metaplastic ectopic bone in the lungs and is less frequent in patients without a clear background of lung diseases. DPO is characterized by very small calcific nodules, often with bone mature located in both lungs and often in peripheral areas of the lungs. Two patterns of DPO have been recognized dendriform and nodular. The dendriform type is less common and is characterized by a coral-like network of bone spiculae along the alveolar septa and is often related to interstitial fibrosis or chronic …

Globularia Alypum L. Modulates Inflammatory Markers In The Human Colon And Shows A Potential Antioxidant Role In Myeloid Leukemic Cells, Najla Hajji, Elodie Hudik, Paola Iovino, Nacim Zouari, Hichem Sebai, Oliver Nusse, Carolina Ciacci

Translational Medicine @ UniSa

Globularia alypum (GA), a plant of the Globulariacea family, has long been used as a traditional cure for inflammatory and metabolic illnesses.In addition to various in vitro model studies, the current work focuses on GA'santioxidant and anti-inflammatory properties in human colon biopsies.

Liquid Chromatography-Electrospray Ionization Mass Spectrometry identified the phenol components in GA aqueous extract (GAAE).The antioxidant ability of GAAE was tested in vitro utilizing chemiluminescence and flow cytometry using fluorescent yeastsn conjunction with PLB-985-human myeloid leukemia cells. Experiments on human colon biopsies after a biopsy challenge with Escherichia coli-lipopolysaccharides aimed tosee if GAAE had an anti-inflammatory impact on human …

The Time For A Consensus Regarding The Coeliac Disease Diagnosis In Adults Has Come: Balancing The Pros And Cons Of Omitting The Biopsy, Carolina Ciacci, Julio C. Bao

Translational Medicine @ UniSa

How balancing the pros and cons of omitting the biopsy in diagnosing CeD in adults?

Grassland Productivity And Water Quality: A 21St Century Issue, David M. Nash, P. M. Haygarth

IGC Proceedings (1977-2023)

Key points

1. Irrigation and other changes to the hydrological cycle can increase soil and water salinity.

2. Primary salinisation is a natural process that affects much of Europe, Asia, Africa, the Americas and Australia. Secondary salinisation is caused by human activities such as irrigation and land clearing that mobilise salt stored in the soil.

3. The critical water contaminants exported from grasslands are nitrogen, phosphorus, potential pathogens and sediment.

4. The mechanisms responsible for diffuse pollution from grasslands and mitigation strategies are most effectively investigated using a ‘source-mobilisation-transport’ framework.

5. There is a lack of coherent interaction across discipline …

Soil Quality Assessment And Management, M. G. Kibblewhite

IGC Proceedings (1977-2023)

Key points

1. Soil quality is related to the capacity of soil to deliver ecosystem services on a sustainable basis.

2. Effective management of soil within grasslands can deliver many benefits to mankind but poor management may cause loss of soil quality from erosion, loss of organic matter, physical deterioration etc.

3. Services are delivered from soil by biological processes. Soil quality depends on the form and condition of the soil habitat. Fixed factors (e.g. texture) are useful for assigning soil to types. Variable factors (e.g. organic carbon) can then be used to assess quality within soil types, by reference …

Water Resources, Agriculture And Pasture: Implications Of Growing Demand And Increasing Scarcity, M. W. Rosegrant, R. A. Valmonte-Santos, S. A. Cline, C. Ringler, W. Li

IGC Proceedings (1977-2023)

Key points

1. Water availability for irrigation is threatened in many regions by rapidly increasing demand for nonagricultural water uses in industry, households, and the environment. The scarcity of irrigation water will not only impact crop production, but also meat production, as much of the pasture used to feed livestock is irrigated.

2. Grassland is caught between two countervailing forces: a requirement for increasing meat demand that boosts the need for additional pasture to support livestock production, and rapidly increasing water scarcity that makes pasture irrigation uneconomical.

3. The most effective means of dealing with water scarcity is likely to …

Soil Microbial Community: Understanding The Belowground Network For Sustainable Grassland Management, Y. G. Zhu, W. D. Kong, B. D. Chen, Z. B. Nan, P. Christie

IGC Proceedings (1977-2023)

Key points

1. In addition to the use of conventional methodologies in soil microbial research, molecular techniques are now being applied to gain insights into the soil microbial community;

2. Plant diversity can exert impacts on soil microbial diversity (through root activities and plant litter etc.), but may in itself be significantly altered by soil properties;

3. Soil microbial diversity largely determines the stability of soil ecosystems under biotic and abiotic perturbations.

4. Management of soil microbial diversity can only be achieved through better understanding their structures and functions.

Characterization Of Streptomyces Nymphaeiformis Sp. Nov., And Its Taxonomic Relatedness To Other Polyhydroxybutyrate-Degrading Streptomycetes, Gary R. Hix, Muhammad S. Khan, Mikayla T. Miller, Elisha C. Napier, Allison L. O'Brien, Roger P. White, Stephen F. Baron Ph.D.

Biology Faculty Scholarship

A polyhydroxybutyrate (PHB)-degrading actinomycete, strain SFB5A^T, was identified as a species of Streptomyces based on its membrane fatty acid profile and the presence of LL-diaminopimelic acid in the cell wall. It formed sporulating mycelia on most agar media, but flat or wrinkled, moist colonies on trypticase soy agar. Spores were smooth, cylindrical, and borne on long, straight to flexuous chains. It produced a light brown diffusible pigment, but not melanin. Comparison of genomic digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values indicated that strain SFB5A^T was related to Streptomyces litmocidini JCM 4394^T , Streptomyces …

Tissue Origin Of Cytotoxic Natural Killer Cells Dictates Their Differential Roles In Mouse Digit Tip Regeneration And Progenitor Cell Survival., Nadjib Dastagir, Zachery Beal, James W Godwin

Faculty Research 2022

Regeneration of amputated digit tips relies on mesenchymal progenitor cells and their differentiation into replacement bone and tissue stroma. Natural killer (NK) cells have well-characterized roles in antigen-independent killing of virally infected, pre-tumorous, or stressed cells; however, the potential for cytotoxic activity against regenerative progenitor cells is unclear. We identified NK cell recruitment to the regenerating digit tip, and NK cytotoxicity was observed against osteoclast and osteoblast progenitors. Adoptive cell transplants of spleen NK (SpNK) or thymus NK (ThNK) donor cells into immunodeficient mice demonstrated ThNK cell-induced apoptosis with a reduction in osteoclasts, osteoblasts, and proliferative cells, resulting in inhibition …

Expanding Homogeneous Culture Of Human Primordial Germ Cell-Like Cells Maintaining Germline Features Without Serum Or Feeder Layers., Mutsumi Kobayashi, Misato Kobayashi, Junko Odajima, Keiko Shioda, Young Sun Hwang, Kotaro Sasaki, Pranam Chatterjee, Christian Kramme, Richie E Kohman, George M Church, Amanda R Loehr, Robert S Weiss, Harald Jüppner, Joanna J Gell, Ching C Lau, Toshi Shioda

Faculty Research 2022

In vitro expansion of human primordial germ cell-like cells (hPGCLCs), a pluripotent stem cell-derived PGC model, has proved challenging due to rapid loss of primordial germ cell (PGC)-like identity and limited cell survival/proliferation. Here, we describe long-term culture hPGCLCs (LTC-hPGCLCs), which actively proliferate in a serum-free, feeder-free condition without apparent limit as highly homogeneous diploid cell populations maintaining transcriptomic and epigenomic characteristics of hPGCLCs. Histone proteomics confirmed reduced H3K9me2 and increased H3K27me3 marks in LTC-hPGCLCs compared with induced pluripotent stem cells (iPSCs). LTC-hPGCLCs established from multiple human iPSC clones of both sexes were telomerase positive, senescence-free cells readily passaged with …