Life Sciences Commons^™

Ecosystem Service Values Of Gardens In The Yellow River Basin, China, Ge Qianqian, Xu Wenjie, Fu Meichen, Han Yingxin, An Guoqiang, Xu Yuetong

Journal of Arid Land

Studies on the ecosystem service value (ESV) of gardens are critical for informing evidence- based land management practices based on an understanding of the local ecosystem. By analyzing equivalent value factors (EVFs), this paper evaluated the values of 11 ecosystem services of gardens in the Yellow River Basin of China in 2019. High-precision land use survey data were used to improve the accuracy of the land use classification, garden areas, and spatial distribution of the ESVs of gardens. The results showed that garden ecosystem generally had high ESVs, especially in terms of the ESV of food production, which is worthy …

Neural Substrates Of Homing Pigeon Spatial Navigation: Results From Electrophysiology Studies, Gerald E. Hough

College of Science & Mathematics Departmental Research

Over many centuries, the homing pigeon has been selectively bred for returning home from a distant location. As a result of this strong selective pressure, homing pigeons have developed an excellent spatial navigation system. This system passes through the hippocampal formation (HF), which shares many striking similarities to the mammalian hippocampus; there are a host of shared neuropeptides, interconnections, and its role in the storage and manipulation of spatial maps. There are some notable differences as well: there are unique connectivity patterns and spatial encoding strategies. This review summarizes the comparisons between the avian and mammalian hippocampal systems, and the …

Crispr-Mediated Multiplexed Live Cell Imaging Of Nonrepetitive Genomic Loci With One Guide Rna Per Locus., Patricia A Clow, Menghan Du, Nathaniel L. Jillette, Aziz Taghbalout, Jacqueline J Zhu, Albert Cheng

Faculty Research 2022

Three-dimensional (3D) structures of the genome are dynamic, heterogeneous and functionally important. Live cell imaging has become the leading method for chromatin dynamics tracking. However, existing CRISPR- and TALE-based genomic labeling techniques have been hampered by laborious protocols and are ineffective in labeling non-repetitive sequences. Here, we report a versatile CRISPR/Casilio-based imaging method that allows for a nonrepetitive genomic locus to be labeled using one guide RNA. We construct Casilio dual-color probes to visualize the dynamic interactions of DNA elements in single live cells in the presence or absence of the cohesin subunit RAD21. Using a three-color palette, we track …

Feasibility Of Tubulin As A Control For Gene Expression Following Transfection In Mouse Monocyte/Macrophage-Like Cells, Ankita Chabra

Honors Program Theses and Research Projects

Transfection, which is the ability to modify host cells’ genetic content, has broad application in studying normal cellular processes, molecular mechanism of disease and gene therapy. There are several transfection techniques, and all require either a control or a reference gene. Commonly used controls for transfection experiments are housekeeping genes, which maintain expression for a given cell/tissue, experimental conditions, and treatment. However, recent research has uncovered that expression levels of housekeeping genes may vary depending on the gene, cell type and experimental conditions. A growing body of evidence demonstrates that housekeeping genes are inadequate internal standards for measuring gene expression …

Farm Succession Planning: Why And How?, Allan Vyhnalek

Cornhusker Economics

If you visit with an ag professional and ask them “What percentage of farmers and ranchers have an estate plan, or an up-to-date estate/succession plan?” the answer will be half, or just less than half. This article will examine why that happens and give some simple steps for anyone to follow to create an up-to-date estate or succession plan. Farmers and ranchers don’t have a plan for a variety of reasons. These might include not ever planning to retire, not wanting to give up control, or not wanting to admit that they will ever pass away. Too often, we don’t …

Nanoparticle Quantification And Distribution On Fluorescently Coated Angioplasty Balloons, Allison Dobson Zieschang

Honors Capstones

No abstract provided.

Lis1 And Ndel1 Regulate Axonal Trafficking Of Mitochondria In Mature Neurons, Jai P. Pandey, Liang Shi, Remi A. Brebion, Deanna S. Smith

Faculty Publications

Defective mitochondrial dynamics in axons have been linked to both developmental and late-onset neurological disorders. Axonal trafficking is in large part governed by the microtubule motors kinesin-1 and cytoplasmic dynein 1 (dynein). Dynein is the primary retrograde transport motor in axons, and mutations in dynein and many of its regulators also cause neurological diseases. Depletion of LIS1, famous for linking dynein deregulation to lissencephaly (smooth brain), in adult mice leads to severe neurological phenotypes, demonstrating post-developmental roles. LIS1 stimulates retrograde transport of acidic organelles in cultured adult rat dorsal root ganglion (DRG) axons but findings on its role in mitochondrial …

The Effects Of A Periodized Strength And Conditioning Program On Overhand Throwing Velocity, Underhand Throwing Velocity And Off The Bat Velocity In Diii Collegiate Athletes, Sage Jenkins

Undergraduate Distinction Papers

Overhand throwing velocity, underhand throwing velocity and off the bat velocity are measures that impact performance of softball athletes. The purpose of strength and conditioning programs is to improve athletic performance for athletes. Strength and conditioning coaches can achieve this through programming. One aspect of programming that this study looked at is periodization. The purpose of this study was to examine the effects of a periodized strength and conditioning program on these performance measurements in NCAA DIII collegiate softball athletes. Sixteen (n=16) NCAA DIII softball athletes were selected based on their participation on the Otterbein softball team. The athletes underwent …

Early Pleistocene Large Mammals From Maka’Amitalu, Hadar, Lower Awash Valley, Ethiopia, John Rowan, Ignacio A. Lazagabaster, Christopher J. Campisano, Faysal Bibi, René Bobe, Jean-Renaud Boisserie, Stephen R. Frost, Tomas Getachew, Christopher C. Gilbert, Margaret E. Lewis, Sahleselasie Melaku, Eric Scott, Antoine Souron, Lars Werdelin, William H. Kimbel, Kaye E. Reed

Publications and Research

The Early Pleistocene was a critical time period in the evolution of eastern African mammal faunas, but fossil assemblages sampling this interval are poorly known from Ethiopia ’ s Afar Depression. Field work by the Hadar Research Project in the Busidima Formation exposures (~2.7 – 0.8 Ma) of Hadar in the lower Awash Valley, resulted in the recovery of an early Homo maxilla (A.L. 666-1) with associated stone tools and fauna from the Maka ’ amitalu basin in the 1990s. These assemblages are dated to ~2.35 Ma by the Bouroukie Tuff 3 (BKT-3). Continued work by the Hadar Research Project …

A Study On The Impact Of Diet On Unified Fire Authority Firefighter Performance, Marin Easton

Student Research Symposium

Objective: This project aimed to examine the impact of nutrition knowledge, dietary intake, and physical activity on firefighters’ performance on their routine physical assessment (RPA) Methodology: Members of the Unified Fire Authority were invited via email to participate in this cross-sectional study. Participants (N=92) completed a 40-question survey that assessed specialized diet adherence, dietary intake, nutrition knowledge (using questions from the General Nutrition Knowledge Questionnaire; 1 point for each correct answer [26 points possible]), regular physical activity (International Physical Activity Questionnaire), and perceived physical performance on their RPA. Based on their responses, participants were categorized into one of two groups: …

The Effects Of Strength And Conditioning Training On The Health Of College Students, Brynli Bleak

Student Research Symposium

Does participation in Strength and Conditioning (S & C) classes have a positive effect on the physical, emotional, mental, and social health of college students? This study was designed to measure and explore different markers of physical, mental, emotional, and social health from participants in Strength and Conditioning classes. Class participants were asked to complete a survey to self report the effects of the training on various aspects of their life. Data were analyzed and compared to national averages for college students, and correlation tests were conducted to identify relationships in the reported effects. The study found that no significant …

Sex Differences In The Fecal Microbiome And Hippocampal Glial Morphology Following Diet And Antibiotic Treatment, Anju Saxena, Roberta R M Moran, Meghan R. Bullard, Emma O. Bondy, Matthew Foster Smith, Lainie Morris, Nicaella Fogle, Jagroop Singh, Brendan Jarvis, Tammy Ray, Juhi Saxena, Linnea Ruth Freeman

Open Access Fund Publications

Rising obesity rates have become a major public health concern within the United States. Understanding the systemic and neural effects of obesity is crucial in designing preventive and therapeutic measures. In previous studies, administration of a high fat diet has induced significant weight gain for mouse models of obesity. Interestingly, sex differences in high-fat diet-induced weight gain have been observed, with female mice gaining significantly less weight compared to male mice on the same high-fat diet. It has also been observed that consumption of a high-fat diet can increase neurogliosis, but the mechanism by which this occurs is still not …

Adapt Lake Mead Releases To Inflow To Give Managers More Flexibility To Slow Reservoir Draw Down, David E. Rosenberg

Publications

A 20-year Colorado River drought continues and Lake Mead draws down. As Lake Mead falls through 8 elevation tiers to 1,020 feet (5.7 million acre-feet [maf]), releases drop and mandatory water conservation targets for California, Arizona, Nevada, and Mexico grow to 1.375 maf per year (USBR, 2019). How will different reservoir inflows, releases, and additional water conservation efforts beyond mandatory targets speed or slow Lake Mead draw down, stabilization, and recovery?

40-Years Of Lake Urmia Restoration Research: Synthesis And Next Steps, Marsoud Parsinejad, David E. Rosenberg, Yusuf Alizade Govarchin Ghale, Bahram Khazaei, Sarah Null, Omid Raja, Ammar Safaie, Somayeh Sima, Armin Sorooshian, Wayne A. Wurtsbaugh

Lake Urmia, Iran Collaborations

Public concern over environmental issues such as ecosystem degradation is high. However, restoring coupled human-natural systems requires integration across many science, technology, engineering, management, and governance topics that are presently fragmented. Here, we synthesized 544 peer-reviewed articles published through September 2020 on the desiccation and nascent recovery of Lake Urmia in northwest Iran. We answered nine questions of scientific and popular interest about causes, impacts, stabilization, recovery, and next steps. We find: (1) Expansion of irrigated agriculture, dam construction, and mismanagement impacted the lake more than temperature increases and precipitation decreases. (2) Aerosols from Lake Urmia's exposed lakebed are negatively …

Genetic Characterization Of Resistance To Bacterial Panicle Blight And Sheath Blight In Rice Using Qtl Linkage Analysis And Qtl-Seq, John Christian E. Ontoy

LSU Master's Theses

Bacterial panicle blight (BPB) and sheath blight (SB) are major diseases of rice causing significant yield reduction in conducive conditions. Few cultivars have shown reliable resistance to these diseases. In this study, a recombinant inbred line (RIL) population derived from the U.S. cultivars Jupiter (moderately resistant) and Trenasse (susceptible) was investigated to identify genomic regions associated with BPB and SB resistance. Phenotypes in BPB and SB, as well as days to heading (DH), of the RIL population were evaluated in the field. DH was found to be correlated to BPB and SB diseases. BPB was also positively correlated to SB. …

Sex And Starvation Influences Latrotoxin Expression In The Brown Widow Spider, Mattie Harris

Honors College Theses

Widow spiders (genus Latrodectus) are well-known for their potent venom. Seven latrotoxin proteins constitute the main components of widow spider venom. The vertebrate specific (α-latrotoxin) and insect specific (α-latroinsectotoxin) latrotoxins have been well-characterized with respect to structure and function. Regulation of latrotoxin gene expression is not well understood but sex and feeding could be factors influencing production. In this study, I used quantitative qPCR to (1) characterize the expression patterns of both the insect and vertebrate specific latrotoxins in male and female brown widow spiders (Latrodectus geometricus) to characterize sex-biased expression and to (2) study expression patterns …

Hsp90-Cdc37 Functions As A Chaperone For The Oncogenic Fgfr3-Tacc3 Fusion, Tao Li, Farideh Mehraein-Ghomi, M Elizabeth Forbes, Sanjeev V Namjoshi, E Ashley Ballard, Qianqian Song, Ping-Chieh Chou, Xuya Wang, Brittany C Parker Kerrigan, Frederick F Lang, Glenn Lesser, Waldemar Debinski, Xuejun Yang, Wei Zhang

Faculty, Staff and Student Publications

The FGFR3-TACC3 (F3-T3) fusion gene was discovered as an oncogenic molecule in glioblastoma and bladder cancers, and has subsequently been found in many cancer types. Notably, F3-T3 was found to be highly expressed in both untreated and matched recurrence glioblastoma under the concurrent radiotherapy and temozolomide (TMZ) treatment, suggesting that targeting F3-T3 is a valid strategy for treatment. Here, we show that the F3-T3 protein is a client of heat shock protein 90 (HSP90), forming a ternary complex with the cell division cycle 37 (CDC37). Deprivation of HSP90 or CDC37 disrupts the formation of the ternary complex, which destabilizes glycosylated …

Brain Charts For The Human Lifespan, R. A. I. Bethlehem, J. Seidlitz, S. R. White, J. W. Vogel, K. Anderson, C. Adamson, S. Adler, G. S. Alexopoulos, E. Anagnostou, John Blangero

School of Medicine Publications

Over the past few decades, neuroimaging has become a ubiquitous tool in basic research and clinical studies of the human brain. However, no reference standards currently exist to quantify individual differences in neuroimaging metrics over time, in contrast to growth charts for anthropometric traits such as height and weight1. Here we assemble an interactive open resource to benchmark brain morphology derived from any current or future sample of MRI data (http://www.brainchart.io/). With the goal of basing these reference charts on the largest and most inclusive dataset available, acknowledging limitations due to known biases of MRI studies …

Flooding Tolerance Of Four Tropical Peatland Tree Species In A Nursery Trial, Hesti L. Tata, Hani S. Nuroniah, Diandra A. Ahsania, Haning Anggunira, Siti N. Hidayati, Meydina Pratama, Istomo Istomo, Rodney Chimner, Meine Van Noordwijk, Randall Kolka

Michigan Tech Publications, Part 1

In order to facilitate hydrological restoration, initiatives have been conducted to promote tree growth in degraded and rewetted peatlands in Indonesia. For these initiatives to be successful, tree seedlings need to be able to survive flooding episodes, with or without shade. We investigated the survival rates and the formation of adventitious roots in the case of four tree species exposed to combinations of different shading and water levels under controlled conditions in a nursery, with artificial rainwater and with peat soil as the medium. The research focused on the following questions (i) whether trees can grow on flooded peat soils; …

Assessing The Influence Of Urban Greening On Urban Arthropods, Brendon Lawrence

Scholars Week

Green Heart is an urban greening experiment in Louisville, KY seeking to create new urban green spaces to ultimately increase air quality and improve human health. Changes in biodiversity in response to urban greening may influence human health via multiple hypothesized pathways including reducing harm (e.g. medicine provisioning), restoring capacities (e.g. reductions in stress), building capacities (e.g. increasing activity outdoors), and causing harm (e.g. zoonotic diseases). Arthropods are one component of diversity that may influence human health via each of these pathways. However, few studies have assessed the influence of Arthropod diversity on human health. Thus, the objective of our …

Nutrient Uptake And Grafting In Ironwood Trees, Tyler Edvall

Student Academic Conference

No abstract provided.

Movement Patterns Of The Shovelnose Guitarfish (Pseudobatos Productus) And California Bat Ray (Myliobatis Californica) In The Southern California Bight, Annabel Gong

Theses

While the movement patterns of large elasmobranch species have been studied extensively, those of smaller, mesopredatory species remain understudied. The shovelnose guitarfish (Pseudobatos productus) and the California bat ray (Myliobatis californica) are among the least studied elasmobranchs in the Southern California Bight. This study quantified the broad- and fine-scale movement patterns of these species using passive acoustic telemetry. Twelve guitarfish were surgically implanted with coded acoustic transmitters at a known aggregation site off La Jolla (San Diego County), California, USA and tracked for 849.5 days each, on average. Six bat rays were also implanted here and tracked for …

Trigonella Foenum-Graecum Methanolic Extract On Isolated Smooth Muscles And Acetylcholinesterase Enzyme: An In Vitro And Mechanistic In Silico Investigation, Muhammad Nabeel Ghayur, Mohnad Abdalla, Asaad Khalid, Saeed Ahmad, Anwar Gilani

Department of Biological & Biomedical Sciences

Results: When tested on the baseline of isolated tissues, Tfg.Cr was devoid of any activity (stimulant or relaxant) till 10 mg/ml. This is an interesting finding, keeping in mind that the fenugreek seeds are used to alleviate constipation and diarrhoea. When Tfg.Cr was tried for any potential AChE inhibitory activity, it did show an inhibitory effect in increasing concentrations (47-380 μg/ml). This inhibitory effect was comparable to the effect produced by a standard AChE inhibitor physostigmine. One of the known fenugreek constituents, diosgenin, was also tested, and it also showed an AChE inhibitory effect in a concentration-dependent manner (11-190 …

Estradiol And Predator Cues Affect Behavior And Brain Responses Of Captive Female House Sparrows, Courtney Harding

Honors Capstones

No abstract provided.

Qualitative Analysis Of Cancer Care Experiences Among Rural Cancer Survivors And Caregivers, Kendra L. Ratnapradipa, Jordan Ranta, Krishtee Napit, Lady Beverly Luma, Tamara Robinson, Danae Dinkel, Laura Schabloske, Shinobu Watanabe-Galloway

Journal Articles

Purpose

Rural (vs urban) patients experience poorer cancer outcomes and are less likely to be engaged in cancer prevention, such as screening. As part of a community needs assessment, we explored rural cancer survivors’ and caregivers’ experiences, perceptions, and attitudes toward cancer care services.

Methods

We conducted 3 focus groups (N = 20) in Spring 2021 in rural Nebraska.

Findings

Three patterns of cancer diagnosis were regular care/screening without noticeable symptoms, treatment for symptoms not initially identified as cancer related, and symptom self-identification. Most participants, regardless of how diagnosis was made, had positive experiences with timely referral for testing (imaging …

Chronic Alcohol Exposure Induces Hepatocyte Damage By Inducing Oxidative Stress, Satb2 And Stem Cell-Like Characteristics, And Activating Lipogenesis, Wei Yu, Yiming Ma, Sushant K. Shrivastava, Rakesh K. Srivastava, Sharmila Shankar

School of Medicine Faculty Publications

Alcohol is a risk factor for hepatocellular carcinoma (HCC). However, the molecular mechanism by which chronic alcohol consumption contributes to HCC is not well understood. The purpose of the study was to demonstrate the effects of chronic ethanol exposure on the damage of human normal hepatocytes. Our data showed that chronic exposure of hepatocytes with ethanol induced changes similar to transformed hepatocytes that is, exhibited colonies and anchorage-independent growth. These damaged hepatocytes contained high levels of reactive oxygen species (ROS) and showed induction of the SATB2 gene. Furthermore, damaged hepatocytes gained the phenotypes of CSCs which expressed stem cell markers …

Healthcare Simulation: A Key To The Future Of Medical Education - A Review, Omair Ayaz, Faisal Wasim Ismail

Medical College Documents

Aim: Simulation originates from its application in the military and aviation. It is implemented at various levels of healthcare education and certification today. However, its use remains unevenly distributed across the globe due to misconception regarding its cost and complexity and to lack of evidence for its consistency and validity. Implementation may also be hindered by an array of factors unique to the locale and its norms. Resource-poor settings may benefit from diverting external funds for short-term simulation projects towards collaboration with local experts and local material sourcing to reduce the overall cost and achieve long-term benefits. The recent shift …

Spongy Moth (Lymantria Dispar Dispar Linnaeus), Ann Mull, Lori R. Spears

All Current Publications

Spongy moths (Lepidoptera: Erebidae) are invasive leaf-eating (defoliating) pests that threaten trees and shrubs in urban, suburban, and rural landscapes. The spongy moth was accidentally introduced to the U.S. in 1869 by an amateur French entomologist in Massachusetts who sought to establish a hardier American silkworm industry. These moths now commonly occur in the northeastern U.S. and are also found in parts of Illinois, Indiana, Michigan, Minnesota, North Carolina, Ohio, Virginia, West Virginia, Wisconsin, California, Oregon, and Washington. In Utah, according to the Utah Department of Agriculture and Food (2021), the spongy moth is anticipated to survive and multiply rapidly …

Fruits Of Nandina Domestica Are (Sometimes) Cyanogenic And (Sometimes) Hazardous To Birds, Scott Zona

Poisonous Plant Research (PPR)

A 12-month survey of cyanogenesis in the fruits and leaves of Nandina domestica (Berberidaceae) revealed that the leaves are strongly cyanogenic throughout the year, as measured by the Feigl-Anger test. Fruits vary in their cyanogenic potential depending on the degree of ripeness and clone. Green fruits are strongly and rapidly cyanogenic, but most ripe fruits are weakly and slowly cyanogenic. Some fruits tested negative for cyanide. Fruits tend to become less cyanogenic the longer they remain on the plant, so birds feeding on older fruits are at a lower risk of poisoning. More than other frugivores, cedar waxwings (Bombycilla …

Local Adaptation Of Blue Penstemon: Molecular And Morphological Characterizations Of A Potential Restoration Species For The Northern Basin And Range And Snake River Plain, Jason Mark Stettler

Theses and Dissertations

Penstemon is one of North America's largest endemic genera with over 280 described species. These species are distributed throughout most of North America from the Arctic northern latitudes to tropics of Central America. The genus has historically been divided into six subgenera, but has recently been reorganized into two subgenera following some recent phylogenetic studies. I made a comprehensive assessment of the Penstemon genus' geographic distribution utilizing herbaria databases by ecoregion to discuss the general ecologic adaptations of each historic subgenera. I also assessed the Penstemon genus' bee pollinator diversity utilizing online databases of bee specimen collections associated with Penstemon …