Life Sciences Commons^™

Identification Of Genetic Elements Involved In Alcaligenes Faecalis’ Inhibitory Mechanism Against Polymicrobial Species, Abigail Mathis

Undergraduate Honors Theses

The rise of antibiotic resistance in common human pathogens and the lack of development of novel therapeutic treatments has created a threat to global health. A unique source for potential novel treatments are from microorganisms, particularly within the complex, antagonistic polymicrobial interactions that take place in microbial communities. These unique mechanisms utilized by microorganisms to fight each other could potentially identify novel therapeutic targets for use at a clinical level, however, there is a lack of research in this area to determine its applicability. Alcaligenes faecalis is a Gram-negative bacterium that seldom causes human disease and has been observed in …

The Validity Of The Vmaxpro During Countermovement Jump And Back Squat Performance, Hunter Haynes

Master's Theses

Background: Advances in technology have resulted in an increase in the utilization of velocity-based training in the strength and conditioning field while utilization of inertia measurement units (IMUs) shows promise. Methods: Recreationally trained participants (N=25, 28.3 ± 2.9 years) were recruited to determine the validity of the VmaxPro device for measuring performance variables in the back squat and countermovement jump (CMJ) against a gold standard force plate. Squat variables assessed included mean concentric velocity (MCV), mean concentric power (MCP), depth, and duration while CMJ variables assessed included MCV, MCP, depth, duration, and jump height. Squat variables were assessed across 3 …

Organ Fat In Latino Youth At Risk For Type 2 Diabetes, Janiel L Pimentel, Kiley B Vander Wyst, Erica G Soltero, Armando Peña, Houchun H Hu, Smita S Bailey, Amber Pokorney, Stephanie L Ayers, Ana Martinez Valencia, Micah L Olson, Gabriel Q Shaibi

Children’s Nutrition Research Center Staff Publications

Purpose: Obesity in youth increases the risk for type 2 diabetes (T2D) and elevated abdominal adipose tissue and organ fat may be particularly deleterious. The purpose of this study was to examine associations among measures of adiposity including total, visceral, and organ fat (hepatic and pancreatic) and whether these measures were independently associated with glycemia in Latino youth at risk for diabetes.

Methods: Latino adolescents (47 boys and 32 girls, 13.7 ± 1.4 years) with obesity (BMIz 2.3 ± 0.3) were assessed for total fat by DXA and visceral and organ fat by 3 T magnetic resonance imaging. Glycemic indicators …

Sustainability Via Active Garden Education: The Sustainability Action Plan Model And Process, Rebecca E Lee, Jacob Szeszulski, Elizabeth Lorenzo, Anel Arriola, Meg Bruening, Paul A Estabrooks, Jennie L Hill, Teresia M O'Connor, Gabriel Q Shaibi, Erica G Soltero, Michael Todd

Children’s Nutrition Research Center Staff Publications

Sustainability of intervention programming is challenging to achieve under real world conditions, since few models exist and many studies do not plan far beyond the funding period. Programming content in early care and education centers (ECECs) is often driven by guidelines. However, implementation is very sensitive to contextual factors, such as the setting and implementer (teacher) characteristics. This paper presents the model, definitions, and methodology used for the sustainability action plan capitalizing on a community-based participatory research (CBPR) approach, developed for a multi-site, multi-level garden-based childhood obesity prevention study, Sustainability via Active Garden Education (SAGE). The Ecologic Model of Obesity …

Toll-Like Receptor 1 Locus Re-Examined In A Genome-Wide Association Study Update On Anti-Helicobacter Pylori Igg Titers, Suk Yee Lam, Michiel C Mommersteeg, Bingting Yu, Linda Broer, Manon C W Spaander, Fabian Frost, Stefan Weiss, Henry Völzke, Markus M Lerch, Ben Schöttker, Yan Zhang, Hannah Stocker, Hermann Brenner, Daniel Levy, Shih-Jen Hwang, Alexis C Wood, Stephen S Rich, Jerome I Rotter, Kent D Taylor, Russell P Tracy, Edmond K Kabagambe, Marcis Leja, Janis Klovins, Raitis Peculis, Dace Rudzite, Liene Nikitina-Zake, Girts Skenders, Vita Rovite, André Uitterlinden, Ernst J Kuipers, Gwenny M Fuhler, Georg Homuth, Maikel P Peppelenbosch

Children’s Nutrition Research Center Staff Publications

Background & aims: A genome-wide significant association between anti-Helicobacter pylori (H pylori) IgG titers and Toll-like receptor (TLR1/6/10) locus on 4p14 was demonstrated for individuals of European ancestry, but not uniformly replicated. We re-investigated this association in an updated genome-wide association study (GWAS) meta-analysis for populations with low gastric cancer incidence, address potential causes of cohort heterogeneity, and explore functional implications of genetic variation at the TLR1/6/10 locus.

Methods: The dichotomous GWAS (25% individuals exhibiting highest anti-H pylori IgG titers vs remaining 75%) included discovery and replication sampls of, respectively, n = 15,685 and n = 9676, all of European …

A Small Stem Assay For Chestnut Blight Resistance In Hybrid American Chestnut Trees, Evelyn Odle

Honors Theses

Cryphonectria parasitica (Murr.) Barr is the causal agent for the chestnut blight disease, which was brought to North America in the late 19th century on nursery stock from Japan (Anagnostakis 1987). A pandemic of chestnut blight lasted for the next half century, nearly wiping out all American chestnut trees (Castanea dentata Borkh.). Restoration works to restoring the American chestnut to its native range in North America’s canopy has been going on for the past century. The American Chestnut Foundation has strived to breed a resistant American chestnut by backcross breeding with Chinese species (C. mollissima Blume) since the 1980s. The …

The Effects Of Dissolved And Suspended Solids On Freshwater Meiofauna, Jessica Cline

Honors Theses

Meiofauna include small-sized animals (< 1mm) distributed in all aquatic ecosystems on Earth, where they play fundamental trophic and ecological roles. The biodiversity of marine meiofauna and its links with anthropogenic activities is routinely investigated, however, freshwater communities are less known. This is particularly true for the Southeastern United States, which is greatly investigated and elected a hotspot of biodiversity for larger species but very little is known about the meiofauna. The purpose of this research is to reveal the biodiversity of meiofauna from the Tennessee River and test for potential correlations with anthropogenic activities. As a proxy for pollution, dissolved and suspended solids were considered in this study. The research hypothesis is that meiofaunal biodiversity would be affected by possible changes of dissolved and suspended solids in the water column. Possible mechanisms causing biodiversity shifts could be ascribed to osmotic stresses of animals to cope with variation in dissolved solids or, more indirectly, because different sunlight penetration caused by suspended solids would affect primary production. To test the hypothesis, water samples were collected from nine stations located along the Tennessee River in Hamilton County. Each station was visited three times, and, during each visit, environmental parameters (including dissolved and suspended solids) were measured. Meiofauna biodiversity (estimated as richness, community composition, and phylogenetic diversity) was revealed using a metagenomic approach. Statistical analyses were applied to test for possible correlations between the biodiversity estimates and the measured environmental parameters. Results show a high biodiversity of meiofauna with more than 200 amplicon-sequence variants distributed across 10 metazoan phyla. Environmental conditions are highly variable among stations and statistical analyses show that while both dissolved solids (TDS) and turbidity (suspended solids, NTU) did not significantly affect meiofauna biodiversity in the collected samples, various other water, and sediment metrics were found to be significant predictors of meiofauna biodiversity. In conclusion, the results of this project not only reveal for the first time the meiofauna biodiversity from the Tennessee River, but also suggest that meiofauna could be used as bioindicators for several anthropogenic activities in freshwater ecosystems.

Role Of Galactose, Thiazolidinediones, And Neet Proteins In Mitochondrial Physiology And Therapeutic Development., Robert Anthony Skolik

Electronic Theses and Dissertations

This dissertation explores the relationship between mitochondrial physiology and development of therapeutics. Mitochondrial dysfunction is associated with both acute and chronic forms of pathophysiology. This work aims to address development efforts at the cell culture and drug-target levels with respect to mitochondria. At the cell culture level, I characterize an approach that has been shown to improve the physiological dependency on mitochondria in tumor-derived cells. I demonstrate that prolonged replacement of glucose with galactose in culture medium induces a global metabolic shift in hepatocellular carcinoma (HepG2) cells to closer reflect a primary hepatocyte phenotype (Chapter 2). I characterize this shift …

The Work Of La Via Campesina Regarding The Intersection Of Land Occupation And Food Sovereignty, Josephine Drydale

International and Global Studies Undergraduate Honors Theses

La Via Campesina (LVC), a global movement that supports small and local farmers and their communities, is one of the world’s largest food and agricultural rights movements and advocates for agricultural rights in terms of land, ecology, human rights, and more. LVC is known for its establishment of food sovereignty, defined as the right to control one’s production and consumption of food. This new concept placed them on the map, accompanied by their aggressive editorial and advocacy work against ideals they view as neoliberal and food policies that benefit large food moguls while disenfranchising the small farmer. The concept of …

The Last Leg: A Social Sustainability Assessment Of Ovine Agriculture, Sydney Golding

International and Global Studies Undergraduate Honors Theses

Post-war agricultural transformations and the rise of Sustainability discourses have dictated the trajectory of sector industries. The implications of agriculture in lateral policy schemes have neglected the careful consideration of the social health of farmers in decision-making processes, creating a greater divide between the interests of the state and our once revered providers. This study aims to capture the complexity of social matters in agriculture within the specific context of sheep farming in the United Kingdom and the United States and how the concurrent systems have adapted considering the impacts of relevant contemporary historical contexts. The principles of Janker, Mann, …

Through The Leaves: Understanding Population Genetic Structure Of Clematis Morefieldii, Keith Andrew Greenway

Honors Capstone Projects and Theses

No abstract provided.

Novel Regulators Of Cellular Secretion Alter The Tumor Microenvironment To Drive Metastasis, Rakhee Bajaj

Dissertations and Theses (Open Access)

Lung cancer is a highly aggressive disease responsible for ~25% of all cancer-related deaths, due in part to its proclivity to metastasize. Treating metastasis holds potential for improving patient survival but requires a deeper investigation into the underlying mechanisms. Some of these processes that can regulate metastasis are: (1) Oncogenic targets of epithelial micro-RNAs (miRNAs) are epigenetically de-repressed upon loss of the miRNAs during epithelial-to-mesenchymal transition (EMT) and in cancer. EMT confers plasticity and fitness to cancer cells promoting their survival through the metastatic cascade. This cascade and EMT are initiated by loss of the miRNA200 family (miR-200) and the …

Parental Stress In Tuberous Sclerosis Complex, Jenny Do

Dissertations and Theses (Open Access)

Tuberous Sclerosis Complex (TSC) is a multi-systemic genetic disorder with great clinical variability. As the needs of one child with TSC may vastly differ from another, parenting demands may similarly differ. Characterizing parental stress, or emotional maladaptation arising from parenting duties, can enable healthcare providers to assist parents of children most efficiently with TSC-related symptoms and improve both parent and child health outcomes. This study surveyed 269 parents of children (aged 0-12 years) with TSC and received the following information: children’s TSC clinical features, parent demographics, and a Parent Stress Index (PSI) score. Parents reported higher stress levels for children …

Prenatal Testing Decisions And Motivations In Pregnancies Conceived Via In Vitro Fertilization, Michelle Appel

Dissertations and Theses (Open Access)

Currently, there is limited information about how conceiving through in vitro fertilization (IVF) and preimplantation genetic testing for aneuploidy (PGT-A) impact the decisions individuals make about prenatal genetic testing. This quantitative study aimed to examine the prenatal testing decisions made by pregnant individuals who conceived via IVF as well as to compare the prenatal testing decisions and motivations between those who had PGT-A and those who did not. An anonymous survey was distributed through online support forums and in clinical settings to eligible individuals. Overall, 230 complete responses were collected with 203 participants far enough along in pregnancy to make …

Atr-Mediated Cd47 And Pd-L1 Upregulation Restricts Radiotherapy-Induced Immune Priming And Abscopal Responses In Colorectal Cancer, Cheng-En Hsieh, Cheng-En Hsieh

Dissertations and Theses (Open Access)

Radiotherapy of colorectal cancer (CRC) can prime adaptive immunity against tumor-associated antigen (TAA)-expressing CRC cells systemically. However, incidences of abscopal tumor remission are extremely rare, and the post-irradiation immune escape mechanisms in CRC remain elusive. We report that CRC cells utilize a common DNA repair signaling pathway — ATR/Chk1/STAT3 — to upregulate both CD47 and PD-L1 in response to radiotherapy, which through engagement of SIRPα and PD-1 suppresses the capacity of antigen-presenting cells to phagocytose them thereby preventing TAA cross-presentation and innate immune activation. This post-irradiation CD47 and PD-L1 upregulation can be observed across various human solid tumor cells. Concordantly, …

Development Of The Ark Assay For Quantitating Dna- Protein Crosslink Accumulation And Fanconi Anemia Pathway Involvement In The Repair Process, Naeh Klages-Mundt

Dissertations and Theses (Open Access)

DNA-protein crosslinks (DPCs) are a common DNA lesion naturally arising in cells, wherein protein becomes covalently and irreversibly bound to the DNA. Given their excessive size, these adducts present a significant challenge to replication and transcription, thus requiring timely and efficient repair. However, the precise mechanisms involved with processing DPC removal remain unclear. Moreover, current methodologies to quantitate DPC accumulation and removal are restrained by a range of limitations. Here, we describe and discuss a new DPC detection assay – the ARK assay – capable of overcoming the limitations incurred by prior assays. The design, which uses dual chaotropic lysis …

Investigating The Ecology And Evolution Of Normal Breast Tissues And Breast Cancer With Single Cell Genomics, Tapsi Kumar, Tapsi Kumar

Dissertations and Theses (Open Access)

There is vast cellular heterogeneity in human breast tissues, with different transcriptional programs in the stromal, epithelial, and immune components, however, it remains unclear how their reprogramming and interplay leads to the progression of invasive phenotypes such as Triple- Negative Breast cancer (TNBC). To do define the microenvironmental alterations that occur during cancer, we first established a human breast cell atlas, a reference of normal breast cell types from disease free women. We profiled 535,941 cells from 62 women and 124,024 nuclei from 20 women revealing 11 major cell types and 52 cell states that reflect different biological functions that …

Stress Effects On Hydrogen Peroxide Defense Response Of Physcomitrella Patens And Mnium Cuspidatum, Caden Karl Groves

Undergraduate Honors Thesis Collection

This project investigated the importance of stresses and the immune system response of hydrogen peroxide formation within the model system Physcomitrella patens, as well as in Mnium cuspidatum. I examined the role of two hormones, abscisic acid and jasmonic acid, along with the role that drought-like conditions play in the specified defense response of hydrogen peroxide production. I hypothesized that abscisic acid would act as an antagonist and reduce hydrogen peroxide production, jasmonic acid would act as an amplifier to increase hydrogen peroxide production and increasing the sodium concentration of the liquid treatment would also act as an antagonist. These …

An Investigation Of Social Ecological Barriers To And Facilitators Of Wic Farmers Market Nutrition Program Voucher Redemption, Renata Blumberg, Emily Fowler, Yeon Bai, Pankaj Lal, Alyssa Smolen, Ilana Dubrovsky

Department of Nutrition and Food Studies Scholarship and Creative Works

In the United States, many communities lack sufficient access to fresh produce. To improve access to fresh fruits and vegetables, the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) provides eligible participants vouchers through the Farmers Market Nutrition Program (FMNP) that can be redeemed directly from farmers at markets or farm stands. However, FMNP voucher redemption rates in New Jersey remain lower than those in neighboring states. This article used the social ecological model to examine differences between FMNP participants who redeem vouchers (Redeemers) and those who do not (non-Redeemers) in the areas of: produce procurement practices and …

Bumpers College Of Agricultural, Food And Life Sciences Professional Development Opportunities And Their Impact On Perceptions Of Career Preparedness, Jessica L. Wesson

Graduate Theses and Dissertations

A student’s undergraduate education is an imperative part of developing career skills that will prepare them to transition from academia to the professional world. Experience outside of the classroom and course material taught in a formal class experience are both important. Their experiences and classes, in college, allow them to develop skills and increase their chances of being successful in the workplace (Suvedi et al., 2016).

Chickering’s Seven Vectors of Student Development were used to help explain the process of student development. A quantitative survey was sent to DBCALFS faculty, staff, and administrators, and a different quantitative survey was sent …

Evaluation Of Current Methods To Detect Cellular Leucine-Rich Repeat Kinase 2 (Lrrk2) Kinase Activity, Belén Fernández, Vinita G. Chittoor-Vinod, Jillian H. Kluss, Kaela Kelly, Nicole Bryant, An Phu Tran Nguyen, Syed A. Bukhari, Nathan J. Smith, Antonio Jesús Lara Ordóñez, Elena Fdez, Marie-Christine Chartier-Harlin, Thomas J. Montine, Mark A. Wilson, Darren J. Moore, Andrew B. West, Mark R. Cookson, R. Jeremy Nichols, Sabine Hilfiker

Department of Biochemistry: Faculty Publications

Background: Coding variation in the Leucine rich repeat kinase 2 gene linked to Parkinson’s disease (PD) promotes enhanced activity of the encoded LRRK2 kinase, particularly with respect to autophosphorylation at S1292 and/or phosphorylation of the heterologous substrate RAB10.

Objective: To determine the inter-laboratory reliability of measurements of cellular LRRK2 kinase activity in the context of wildtype or mutant LRRK2 expression using published protocols.

Methods: Benchmark western blot assessments of phospho-LRRK2 and phospho-RAB10 were performed in parallel with in situ immunological approaches in HEK293T, mouse embryonic fibroblasts, and lymphoblastoid cell lines. Rat brain tissue, with or without adenovirus-mediated …

Population Genomics Provides Insights Into The Genetic Basis Of Adaptive Evolution In The Mushroom-Forming Fungus Lentinula Edodes, Jingcheng Zhang, Nan Shen, Chuang Li, Xingjie Xiang, Gaolei Liu, Ying Gui, Sean Patev, David S. Hibbett, Kerrie Barry, William Andreopoulos, Anna Lipzen, Robert Riley, Guifen He, Mi Yan, Igor V. Grigoriev, Hoi Shan Kwan, Man Kit Cheung, Yinbing Bian, Yang Xiao

Biology

Introduction: Mushroom-forming fungi comprise diverse species that develop complex multicellular structures. In cultivated species, both ecological adaptation and artificial selection have driven genome evolution. However, little is known about the connections among genotype, phenotype and adaptation in mushroom-forming fungi. Objectives: This study aimed to (1) uncover the population structure and demographic history of Lentinula edodes, (2) dissect the genetic basis of adaptive evolution in L. edodes, and (3) determine if genes related to fruiting body development are involved in adaptive evolution. Methods: We analyzed genomes and fruiting body-related traits (FBRTs) in 133 L. edodes strains and conducted RNA-seq analysis of …

The Potential Impact Of Climate Change On The Distributions Of Eleutherodactylus Cystignathoides And E. Planirostris (Anura: Eleutherodactylidae), Rebecca T. Chastain

Theses and Dissertations

Climate change is inducing changes in the distributions of many species, causing range shifts and habitat loss as well as facilitating invasions. It is a broad contributor to global amphibian decline, already causing mass extinctions and extirpations of amphibian populations, a phenomenon which is expected to continue. The understudied direct-developing frog Eleutherodactylus cystignathoides is a notable outlier, having rapidly expanded its distribution in recent years. This is of particular interest given that other eleutherodactylids, such as E. coqui and E. planirostris, have already shown propensity for rapid dispersal and invasion. Originally found in the southernmost tip of …

Wayne E. Sabbe Arkansas Soil Fertility Studies 2021, Nathan A. Slaton

Arkansas Agricultural Experiment Station Research Series

Rapid technological changes in crop management and production require that the research efforts be presented in an expeditious manner. The contributions of soil fertility and fertilizers are major production factors in all Arkansas crops. The studies described within will allow producers to compare their practices with the university’s research efforts. Additionally, soil-test data and fertilizer sales are presented to allow comparisons among years, crops, and other areas within Arkansas.

Oestrogenic Subterranean Clover Guide. Identification And Remediation, Kevin J. Foster, Megan H. Ryan, Daniel R. Kidd

Bulletins 4000 -

Subterranean clover (subclover, Trifolium subterraneum L.) is an annual pasture legume that provides high quality feed for livestock. It is estimated to be sown over 30 million hectares across southern Australia, with cultivars developed for the low, medium and high rainfall zones. Unfortunately, some older cultivars contain high levels of phytoestrogen which can affect the reproductive system of sheep and cause other health issues. This became a significant issue for sheep producers from the 1940s as new land was cleared and sheep grazed subclover-dominant pastures. There was a collection of livestock issues observed and these were grouped together under the …

Fish Community Assembly At Multiple Spatial Scales In East Texas Streams, Jake R. Swanson

Electronic Theses and Dissertations

Stream communities are structured by environmental processes that vary in strength across different spatiotemporal scales. Therefore, revealing how stream fish communities are influenced by environmental gradients may clarify how communities respond to disturbance associated with anthropogenic change. I investigated the relative role environmental variables play in the processes that structure the taxonomic and functional diversity of stream fish communities, as well as historical trends in occurrence and local habitat associated with a species of greatest conservation need within east Texas. The results of this study indicate that stream sites with more habitat complexity and stability support a more taxonomically and …

Multi-Trophic Biodiversity Increases With Increasing Structural Complexity Of Forest Canopy, Ayanna St. Rose

Graduate Theses and Dissertations

Understanding the effects of forest canopy structural complexity on multi-trophic diversity is critical for conserving biodiversity and managing land sustainably. But multi-trophic diversity is often ignored when making decisions about land management due to lack of cost- and time-effective methods to evaluate it. Here, we explored a new method based on widely available remote sensing data to quantify canopy structural complexity and its relationships with multi-trophic biodiversity at landscape scale using 32 forested sites of the National Ecological Observatory Network. We investigated the influence of vertical and horizontal structural complexity of forest canopy on multi-trophic (primary producers, herbivores (beetles), omnivores …

Generation Of Baiap3 Expression Construct For Subcellular Localization And Phenotypic Rescue, Ireland Ann Little

Honors Theses

Mast cells secrete a variety of biological mediators crucial for innate and adaptive immunity. One such mediator is Tumor Necrosis Factor (TNF). TNF released from mast cells and other immune cells has been shown to elicit complex signaling pathways in target cells, resulting in the production of pro-inflammatory cytokines and/or immune cell recruitment. At the molecular level, TNF secretion/exocytosis is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and other regulatory proteins that work together to promote the fusion between secretory granules and the plasma membrane. One regulatory protein, Munc13-4, which facilitates the priming of secretory granules, was recently …

World Association For The Advancement Of Veterinary Parasitology (Waavp): Second Edition Of Guidelines For Evaluating The Efficacy Of Anthelmintics In Poultry, T. A. Yazwinski, J. Hoglund, A. Permin, M. Gauly, C. Tucker

Animal Science Faculty Publications and Presentations

This revision of the original poultry guidelines has been prepared to assist in the planning, conduct and interpretation of studies designed to assess the anthelmintic efficacy of drugs (newly discovered or currently used) against helminth parasites of chickens and turkeys. The original set of poultry guidelines was published in 2003. The current version provides an update on procedures to study and quantify the most important helminth parasites of chickens and turkeys, and to integrate these poultry guidelines with a new series of general, reflective and host-specific guidelines relative to assessing anthelmintic efficacy in production and companion animals. General considerations required …

Predicting The Progression Of Diabetes Mellitus Using Dynamic Plantar Pressure Parameters, Mathew Sunil Varre

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: Diabetic peripheral neuropathy is one of the common complications of type-2 diabetes mellitus (DM). Changes in the intrinsic plantar tissue coupled with repetitive mechanical loads and loss of sensation may lead to foot related complications (skin break down, ulcerations, and amputations) in persons with neuropathy if left untreated. The purpose of this dissertation was to stratify individuals with pre-diabetes, diabetes with and without neuropathy using dynamic plantar pressure parameters during walking, using machine learning algorithms.Methods: Plantar pressure data was collected from one hundred participants during walking with pressure measuring insoles fixed between the feet and thin socks. Simultaneously high-definition …