Life Sciences Commons^™

Characterization Of The Cell Cycle Dependent Kinase Inhibitor P27kip1 In The Stem-Like Properties Of Breast Cancer Stem Cells, Jaz-Mine Mccovins

Theses (2016-Present)

Breast cancer is the second leading cause of cancer death among American women. Traditional therapies, such as chemotherapy and radiation therapy, target proliferative cells within the tumor bulk. However, these treatments do not provide long-term disease survival because they fail to eliminate a small population of long-lived tumor initiating cells known as breast cancer stem cells (CSCs). Two features of CSCs are that they slowly divide and are more resistant to conventional anti-cancer treatments due to altered cellular machinery. It is the breast CSC subpopulation that develops new tumors which have the potential to lead to recurrence. However, the mechanism …

Experimental Validation Of Gene Expression Of Mybl1, Mybl2, Ubxn8, And Adrm1 Genes In Triple Negative Breast Cancer Cell Lines, Esther Jensinne Nsende

Theses (2016-Present)

A previous study conducted in our laboratory demonstrated V-Myb Avian Myeloblast Viral Oncogene Homolog Like 1 (MYBL1) gene over-expression in triple negative breast cancer (TNBC) compared to normal, some luminal, and a subpopulation of other TNBC. The MYBL1 gene belongs to the Avian myeloblastosis virus (MYB) family and is classified as proto-oncogene that functions as a strong transcription factor. The MYBL1 gene is related to cancer progression which involves dysregulation of cell cycle signaling, apoptosis and differentiation processes. A primary goal of our laboratory is to further characterize MYBL1 gene expression in TNBC samples. To achieve this goal, we performed …

A Most Unusual Tail: Scoliosis In A Wild Australian Skink, And Reported Incidences And Suggested Causes Of Similar Malformations Amongst Squamates, Philip W. Bateman, Robyn Benken, Ryan Glowacki, Robert A. Davis

Research outputs 2022 to 2026

Malformations of vertebrae potentially occur across many taxa, particularly in Testudines, which are susceptible to kyphosis (dorsoventral curvature of the spine). Such malformations may stem from either genetic or environmental origins, and their prevalence and associated impacts on survival remain poorly understood. However, scoliosis (sideways curvature of the spine) is rarely reported and especially so in wild lizards. We report here on the first known case of scoliosis in a wild Australian lizard, the skink Ctenotus fallens, from Perth, Western Australia. This occurrence is the first of 805 individuals captured in a natural population monitored annually for the past 11 …

Burden Of Food Insecurity Among College Students: A Cross-Sectional Study During The Covid-19 Pandemic, Saba Sami

Electronic Theses, Projects, and Dissertations

Background: Food insecurity is lack of access to proper nutrition for an individual to obtain a healthy lifestyle. The goal of this research is to determine the frequency of food insecurity among college students at a four-year college institution during COVID-19 pandemic in association to mental and physical health status.

Methods: This study was conducted among college students using cross-sectional study method. All data were collection virtually and kept anonymous. All statistical analyses were conducted using SPSS version 28 with (p<.05), denoting significance.

Results: In the study there were a total of 98 participants who were being analyzed. All the participants were …

Native And Non-Native Ant Impacts On Soil Microbes, Hannah A. Stewart

Biology Theses

Organisms produce chemical weapons for defense, but target organisms can develop resistance. In their introduced range, non-native species may bring “novel weapons” against which native organisms have not co-evolved resistance. The invasive European fire ant (Myrmica rubra) may have brought antimicrobial secretions to the Northeastern United States that are novel weapons against native fungal and bacterial soil organisms. I hypothesized that M. rubra would better inhibit seed pathogens resulting in greater emergence of native myrmecochorous Viola sororia seeds and, as a side effect, more strongly inhibit arbuscular mycorrhizal fungi than a native seed dispersing ant (Aphaenogaster picea …

Quantification Of Dental Fissures Through Occlusal Topography Analysis, Duru Erkan

Biological Sciences Undergraduate Honors Theses

Narrow, deep, and elaborate dental fissures are widely believed to increase the predisposition of enamel to develop carious lesions as these surfaces are thought to be ideal for cariogenic bacteria to adhere to. Therefore, dentists prescribe sealants as a preventative measure for patients with such fissures to avoid tooth decay. Yet to date, there is no objective data supporting the notion that fissure morphology actually affects caries susceptibility, nor is there a quantifiable, clinically practical method to characterize fissure patterns expected to increase risk of caries disease.

In this study, three new methods to quantitatively characterize fissure pattern in mandibular …

Structural Formation And Functionality Of The Spoiie Protein In Clostridium Difficile, Rachel Siebenmorgen

Biological Sciences Undergraduate Honors Theses

My research efforts were directed toward the structure and functionality of a protein, designated SpoIIE [11], which previously has been shown to regulate C. difficile peptide utilization in addition to sporulation and toxin release. The SpoIIE protein is found in all spore-forming gram-positive bacteria and is a crucial component in activation of transcription factors and septum formation thus affecting the regulation and timing of spore development [11,12]. A truncated form of the SpoIIE protein named SpoIIE∆TM was used in an effort to determine the structure of an important but uncharacterized linker domain of the protein. If the structure and specific …

Examining The Invasion Of Bush Honeysuckle Through A Phylogenetic Analysis, Phoebe Bruffett

Biological Sciences Undergraduate Honors Theses

The mechanisms underlying the continual spread of invasive plants within their respective non-native ranges is a major focal point to invasion research. Many theories have been proposed to understand these invasions, each with different implications for the predicted range for invasive plants. Lonicera maackii provides an exceptional opportunity to examine the effectiveness of these theories to explain the trends of invasive plants. Lonicera maackii is native to eastern Asia, but has invaded much of the eastern United States, presenting a severe threat to the health of forest and other natural areas. Recent analysis of the climatic envelopes uncovered a significant …

Investigating The Potential Role For The Nervous System In Controlling Regeneration In Nematostella Vectensis, Kristen Malir

Biological Sciences Undergraduate Honors Theses

Nematostella vectensis is a marine sea animal that has become a model for developmental and evolutionary research. Included in the phylum Cnidaria, N. vectensis’ was chosen as the model for this research. Not only can this animal go through asexual and sexual reproduction, but it also has the ability to regenerate. Although much research has been put forth in an effort to understand regeneration better, much is still unknown. The underlying mechanisms of regeneration in Cnidaria are illusive; however, studies within vertebrates have shown the substantial role of the nervous system. The objective of this experiment is to test if …

Examining Difference In Social Perceptions Between Women Using Hormonal Contraceptives And Naturally Cycling Women, Caroline B. Johnson

Biological Sciences Undergraduate Honors Theses

The term “stress” refers to a person’s psychological and physiological response to the demands and pressures of the world around them (Farlex, 2021). Past research has shown that stress can have negative side effects on a person’s well-being (Aneshensel et al., 1991; Wunsch et al., 2017; Michie, 2002). Although people experience stress, some people perceive more stress than others. Perceptions are important because the way one understands certain conditions can elicit distinct emotional and physiological responses (Kemeny, 2003). An important factor that has not received a lot of attention is women’s use of hormonal contraceptives. In the United States, 24.4% …

The Synchronization Of Nocturnal Pollinator Behavior And Apple Flower Nectar Production, Madison Jennings

Biological Sciences Undergraduate Honors Theses

Insects perform an essential ecological service by facilitating the pollination of crops for food production worldwide. Recent declines in diurnal bee populations threaten food security and has led to growing concerns about existent pollination methods. Pollination contributions by native nocturnal insects have been documented to occur in a variety of systems, thought to be supported by the plant’s attractant and reward system. If this is the case, pollination of flowers by nocturnal vectors may be influenced by circadian cycles of nectar production and insect activity. To test this hypothesis, we recorded insect abundance of nocturnal pollinators periodically throughout the night …

Characterization Of Fgf1/Fgf2 Heterodimer With Mutation R136e, Brett Haley

Biological Sciences Undergraduate Honors Theses

Fibroblast growth factors are a family of heparin binding proteins that are involved in a broad spectrum of physiological functions, such as angiogenesis, cell proliferation, cell differentiation, and wound healing. Fibroblast growth factor 1 (FGF-1) is a member of the FGF superfamily and can bind universally to fibroblast growth factor receptors. Fibroblast growth factor 2 (FGF-2) is similar in structure to FGF-1 but has been shown to have a potent effect on angiogenesis. A physiologically stable dimerization of these two growth factors could prove to induce desirable effects in the instance of wound healing. In this study, we have designed …

Regulation Of The Reaction Between Cytochrome C And Cytochrome C Oxidase In The Mitochondria, Anders Nowell

Biological Sciences Undergraduate Honors Theses

Cytochrome c (Cc) is a multifunction protein that has important life and death functions in the cell. In the electron transport chain (ETC), Cc transfers electrons from cytochrome bc₁ to cytochrome c oxidase (CcO), which helps build the electrochemical gradient that drives ATP synthase. The reaction of Cc with CcO is very important in ETC regulatory processes. Previous research shows phosphorylation sites in Cc that affect the binding with CcO, with measurable effects on k_d, k_f, and K_D. These effects result in changes in mitochondrial membrane potentials, respiration, and reactive oxygen species (ROS) …

Using Ai To Examine Nocturnal Moth Pollination, Conor Moriarty

Biological Sciences Undergraduate Honors Theses

The importance of insect-mediated agriculture cannot be understated especially in the wake of pollinator population declines. Most research of insect-mediated pollination is focused on diurnal pollinators like the honeybee (Apis mellifera). Deepening our understanding of how other pollinators, such as nocturnal pollinators, can benefit agriculture and ecosystems will be very important as honeybee populations decline. I explored nocturnal moth interactions with flowering apple flowers during their pollination season to better understand how these nocturnal pollinators interact with the plants. To accomplish this, I used a University of Arkansas farm area with a dedicated apple orchard. I gathered my raw data …

The Role Of Dynamic Gaze Fixations In Human Postural Control, Grace Balsam

Health, Human Performance and Recreation Undergraduate Honors Theses

Background: The human postural control system, which controls balance, is constantly taking in sensory input to help maintain balance while allowing one to pay attention to their daily activities. Using visual search tasks, we will be able to understand information relevant to how changes in visual input affects one’s postural control representative of a more real-life scenario. Purpose: With the help eye tracking technology, this study assesses changes in postural control during various visual conditions. Postural control will be measured during such tasks to analyze how it compares to a simple, quiet balancing task. Following Bonnet and Baudry’s …

Self-Rated Function And Delayed Onset Muscle Soreness (Doms) Responses To 6-Week Supplementation With Waters With Anti-Inflammatory Capabilities (Wac), Kristin Garner

Health, Human Performance and Recreation Undergraduate Honors Theses

Introduction: Researchers have examined the effects of sedentary lifestyles on diminished health outcomes and are pleading individuals to take on a more active lifestyle to prevent these. However, initial efforts to exercise are often dampened by delayed onset muscle soreness (DOMS) and muscle fatigue. This can greatly reduce exercise adherence, making it difficult for individuals to overcome this temporary discomfort. Background: Research has extensively evaluated nonsteroidal anti-inflammatory drugs (NSAIDs) and fruits as anti-inflammatory supplementation to treat DOMS. Berry-derived essences are more highly concentrated in anti-inflammatory substances than regular fruit juices, but there is minimal research on their effects on exercise …

Exploration Of Genes Controlling Grain Yield Heterosis In Hybrid Wheat (Triticum Aestivum L.) Utilizing 3ʹ Rna Sequencing, Nichole Lynn Miller

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The implementation and future success of hybrid wheat (Triticum aestivum L.) is impacted by breeders’ inability to create consistent high yielding, high heterosis hybrids. This research addresses this problem by conducting an exploration of transcriptomes from hybrids and parent lines to determine what genes are active in heterotic or non-heterotic hybrids and how their level of expression can explain the phenotype of grain yield heterosis. Using hybrids that showed positive mid-parent heterosis (MPH), classified as heterotic in our study, and negative or no difference MPH hybrids, classified as non-heterotic, differentially expressed genes (DEGs) potentially related to heterosis and hybrid …

Direction Of Attentional Focus In Prosthetic Training: Current Practice And Potential For Improving Motor Learning In Individuals With Lower Limb Loss, Alexander Bonczyk, Maria Katrina Tillada Dimapilis, Sarah Partridge, Samantha Ruiz

UNLV Theses, Dissertations, Professional Papers, and Capstones

Objective: Adopting an external focus of attention has been shown to benefit motor performance and learning. However, the potential of optimizing attentional focus for improving prosthetic motor skills in lower limb prosthesis (LLP) users has not been examined. In this study, we investigated the frequency and direction of attentional focus embedded in the verbal instructions in a clinical prosthetic training setting.

Methods: Twenty-one adult LLP users (8 female, 13 male; 85% at K3 level; mean age=50.5) were recruited from prosthetic clinics in the Southern Nevada region. Verbal interactions between LLP users and their prosthetists (mean experience=10 years, range=4-21 years) during …

Effects Of A Remote Exercise Intervention On Aerobic Endurance In Individuals With Down Syndrome, Andrew Murata, Elena Wolf, Jonathan Crimm, Benjamin Lee

UNLV Theses, Dissertations, Professional Papers, and Capstones

Purpose: Down syndrome (Ds) or Trisomy 21 is a common genetic birth condition, and those with Ds typically display decreased cardiorespiratory fitness compared to those without Ds, potentially leading to an increased risk for cardiovascular conditions and mortality. Low cardiorespiratory fitness is partially caused by low physical activity levels in this population. The COVID-19 pandemic caused community-based activity programs to shut down, as individuals with Ds are at increased risk for COVID-19 hospitalization and death. Thus, it was vital to explore alternatives of in-person exercise during the pandemic. The purpose of this study was to investigate the effects of a …

Research Report: High Tunnel Tomato Fruit Cluster Pruning, Caterina Roman, Rebecca G. Sideman

Faculty Publications

Tomatoes are a high value crop grown worldwide. Indeterminate varieties are commonly grown in high tunnel structures throughout New England for the fresh market. Indeterminate tomato plants often suffer from a phenomenon called ‘June drop’ in which the plant’s first four to five cluster of fruit set perfectly but the subsequent two to three clusters have poor set and plant productivity drops suddenly. While cluster thinning (e.g., reducing the number of fruit allowed to mature per cluster) has been successfully shown to increase fruit size, it has generally not increased marketable yield. We hypothesized that reducing the fruit load by …

Impact Of Climate Change On Hydrochemical Processes At Two High-Elevation Forested Watersheds In The Southern Appalachians, United States, Hailong Huang, Wei Wu, Katherine Elliott, Chelcy Miniat, Charles Driscoll

Faculty Publications

Climate change increasingly affects primary productivity and biogeochemical cycles in forest ecosystems at local and global scales. To predict change in vegetation, soil, and hydrologic processes, we applied an integrated biogeochemical model Photosynthesis-EvapoTranspration and BioGeoChemistry (PnET-BGC) to two high-elevation forested watersheds in the southern Appalachians in the US under representative (or radiative) concentration pathway (RCP)4.5 and RCP8.5 scenarios. We investigated seasonal variability of the changes from current (1986–2015) to future climate scenarios (2071–2100) for important biogeochemical processes/states; identified change points for biogeochemical variables from 1931 to 2100 that indicate potential regime shifts; and compared the climate change impacts of a …

Exploring The Effects Of Varied Land Use On Elemental Concentrations Within Streams, Logan Jennings

Biological and Agricultural Engineering Undergraduate Honors Theses

It is well documented that human activity influences the chemistry of surrounding waters. As such, it is possible that there is a link between land use within a watershed and the chemical composition of the stream. The objectives of this study are to determine if varied land use does affect the concentrations of macronutrients and trace elements present in the streams of Northwest Arkansas, and if so, to determine what extent urban and agricultural development are responsible for these changes. Water samples were collected across 19 streams in the Northwest Arkansas region between January and March of 2022. Water samples …

Potato Processing In The Home: Sustainability And Nutritional Impacts, Aubin Payne

Biological and Agricultural Engineering Undergraduate Honors Theses

Potatoes are the world's fourth most consumed crop. Their versatility and long shelf-life make them a staple food for millions of people worldwide. Still, the increasing consumption of highly processed potato products in developed countries has damaged the public's appreciation of potatoes as a valuable source of essential nutrients. Additionally, as public awareness of environmental sustainability increases, the average consumer is more likely to value processing methods that mitigate environmental damage.

Researchers simulated several home storage and processing conditions to find out how nutrition and environmental impact are affected by the home processing timeline. Then, a nutrient analysis, life cycle …

Development Of An Equation To Correlate Brite Tank Temperature And Pressure To Beverage Carbonation Concentration, Devyn Meyer

Biological and Agricultural Engineering Undergraduate Honors Theses

The Carbo Rock-It™ is an invention created to carbonate beverages at craft breweries. One of the Carbo Rock-It’s main features is that it does not add a significant volume of undissolved gas bubbles to the brite tank in which the beverage is carbonated. This may allow temperature and pressure readings from the brite tank to be used to determine the concentration of dissolved carbon dioxide in the beverage continuously in real time. This eliminates the need for manual samplings, which could save breweries time and allow for more controlled, accurate carbonation. The goal of this project was to develop an …

Biophysical Insights Into The Transport Properties Of Lysenin Channels, Andrew Merlin Bogard

Boise State University Theses and Dissertations

The defense work focused on deciphering novel functionalities of lysenin channels with respect to adjustment of their regulation and transport properties in response to environmental changes. Lysenin is a pore-forming toxin extracted from the earthworm E. fetida, which self-assembles into large pores in artificial and natural lipid membranes containing sphingomyelin. Prior investigations on their functionality identified strong regulation by voltage and ligands as fundamental traits of lysenin, similar to ion channels. In addition, stochastic sensing, controlled switching, and hysteretic conductance have been added to the list of intricate biophysical properties of lysenin channels as potential enablers of biotechnological and biomedical …

Effects Of Vegetation Heterogeneity On Multiphasic Treatment Outcomes In Sagebrush Steppe, Rebecca Donaldson

Boise State University Theses and Dissertations

Exotic annual grass invasion into western North America has led to significant loss of native perennials, altering the structure and function of sagebrush-steppe ecosystems. Monitoring and assessment of necessary restoration treatments have provided mixed evidence of success. We hypothesized that treatment outcomes would be influenced by restoration strategy (e.g., the timing of herbicide or drill seeding) and by within-treatment vegetation heterogeneity. We evaluated exotic annual grass and exotic perennial forb response to three replicate treatments of the pre-emergent herbicides indaziflam and imazapic, and a combination treatment of both herbicides, followed with the broadleaf herbicide, aminopyralid, at a highly invaded site …

Sex, Body Size, And Winter Weather Explain Migration Strategies In A Partial Migrant Population Of American Kestrels (Falco Sparverius), Sadie Claire Ranck

Boise State University Theses and Dissertations

Partially migratory systems describe populations that consist of both individuals that migrate away from the breeding grounds for the winter, and others that remain resident near their nesting sites year-round. Partial migration is the most common type of migration across all animal taxa, but the evolution, maintenance, and consequences associated with different movement strategies are still poorly understood. Studying the factors that drive migratory strategies and the associated consequences of those decisions is important to understand how migratory animals may adapt to climate change. Partial migrant populations offer a great opportunity for which to study these questions because individuals with …

The Effects Of Roads On Movement, Flight Dynamics, Occupancy, And Productivity In Barn Owls (Tyto Alba), Brian Thomas Busby

Boise State University Theses and Dissertations

Barn Owls (Tyto alba) are a species of conservation concern in many portions of their cosmopolitan range. One important factor contributing to population declines and sometimes local extirpations is roads, which can cause direct mortality through Barn Owl-vehicle collisions, fragment habitat, limit dispersal and movement, and imperil long-term population viability. However, the effects of roads on Barn Owl reproduction are less clear. Further, the cumulative effects of roads on Barn Owls can be dependent on how they respond to them. Road and traffic responses of animals have been classified into four categories: 1) speeders, who increase speed to …

Emerging Roles Of Intragenic Enhancers In Gene Expression Of Gadd45a, Ekaterina Sazon

Biological Sciences

Non-coding regulatory elements in the eukaryotic genomic DNA, like enhancers and promoters, control gene expression. Transcription factors recognize and bind to DNA sequences within these regulatory regions and affect said regulatory elements. The extent and specific circumstances regarding transcription factors are long-standing questions in the field. In this thesis, we wanted to investigate how transcription factors work in a particular case of enhancers, known as intragenic enhancers. We designed reporter gene assays that looked at the well-studied model transcription factor p53’s regulation on its target gene GADD45A in basal and stress conditions. We determined that GADD45A is a p53-dependent gene, …

Toward Long Non-Coding Rna Detection Using Dna Nanoswitches, Ché-Doni Platt

Biological Sciences

Long non-coding RNAs (lncRNAs) are transcripts that are over 200 nucleotides and do not encode proteins. LncRNAs are involved in a wide spectrum of biological processes ranging from cell proliferation, apoptosis, and nutrient sensing to cell differentiation. Further, lncRNAs have been reported to play an important role in a wide range of pathophysiological processes and are linked to diseases such as cancer. Thus, lncRNAs are becoming increasingly important as biomarkers to study biological and disease processes. In our work, we develop a DNA nanoswitch-based assay for detecting long RNAs. The DNA nanoswitch is a reconfigurable device that undergoes a conformational …