Life Sciences Commons^™

Two High Hierarchical Regulators, Pumyb40 And Puwrky75, Control The Low Phosphorus Driven Adventitious Root Formation In Populus Ussuriensis, Hanzeng Wang, Solme Pak, Jia Yang, Ye Wu, Wenlong Li, He Feng, Jingli Yang, Hairong Wei, Chenghao Li

Michigan Tech Publications, Part 1

Adventitious rooting is an essential biological process in the vegetative propagation of economically important horticultural and forest tree species. It enables utilization of the elite genotypes in breeding programmes and production. Promotion of adventitious root (AR) formation has been associated with starvation of inorganic phosphate and some factors involved in low phosphorus (LP) signalling. However, the regulatory mechanism underlying LP-mediated AR formation remains largely elusive. We established an efficient experimental system that guaranteed AR formation through short-term LP treatment in Populus ussuriensis. We then generated a time-course RNA-seq data set to recognize key regulatory genes and regulatory cascades positively regulating …

Effect Of Mycorrhizae Inoculation On The Growth And Success Of Three Taxodium Distichum Hybrids In Saline - Impacted Coastal Soils, Elif Ilhan

Electronic Theses and Dissertations

In 2008, Galveston Island was severely impacted by Hurricane Ike, resulting in high salt deposition in the soil and groundwater. This caused a loss of many native plant species. A study was initiated to determine effective ways to promote the growth conditions of three bald cypress genotypes (Taxodium distichum var distichum and Taxodium distichum var mexicanum crosses) in salt-affected soils. The treatments applied were mycorrhizae inoculation, fertilizer application, and a combination of mycorrhizae inoculation and fertilizer application. A total of sixty (60) trees planted in plots of three rows and divided into five randomized replication blocks of four treatments …

Electromechanical Fatigue Properties Of Dielectric Elastomer Stretch Sensors Under Orthopaedic Loading Conditions, Andrea Karen Persons

Theses and Dissertations

Fatigue testing of stretch sensors often focuses on high amplitude, low-cycle fatigue (LCF) behavior; however, when used for orthopaedic, athletic, or ergonomic assessments, stretch sensors are subjected to low amplitude, high-cycle fatigue (HCF) conditions. As an added layer of complexity, the fatigue testing of stretch sensors is not only focused on the life of the material comprising the sensor, but also on the reliability of the signal produced during the extension and relaxation of the sensor. Research into the development of a smart sock that can be used to measure the range of motion (ROM) of the ankle joint during …

When Feminism Isn't Enough, Monica H. Green

Medieval Feminist Forum: A Journal of Gender and Sexuality

An invited “retrospective” on Monica H. Green's career in medieval studies.

Quagga Mussel And Zebra Mussel, Ann Mull, Lori R. Spears

All Current Publications

Quagga mussel and zebra mussel can cause significant ecological, economical, and recreational impacts. This fact sheet describes these two-sided mollusks and reviews impacts, monitoring, and management of these invasive species.

Evaluating The Effect Of Coated Urea And Urea-Ammonium-Nitrate Solution On Cane Tonnage, Sugar Yield, And Leaf-Soil Nitrogen Content Under Louisiana Sugarcane Production System, Hector J. Mendoza Lagos

LSU Master's Theses

Nitrogen (N) is a crucial nutrient for proper plant growth and development. N deficiency results in poor tillering and thin, short internode stem production for sugarcane production, thus resulting in low cane tonnage. Therefore, proper N fertilizer management is essential for a sustainable sugarcane production system. This research was conducted at the LSU AgCenter Sugar Research Station in St. Gabriel, LA, from 2019 to 2020 at three Sites (Site 1, 2, and 3). The treatments consisted of different N rates (0, 45, 90, and 135 kg N ha^-1) and sources (urea-ammonium-nitrate solution [UAN]-32%, 30-day [Coated Urea 1], 45-day …

Cardiovascular And Range Of Motion Effects After Exercise Training Interventions, Nolynn E. Sutherland

LSU Doctoral Dissertations

The effects of stretching and yoga on the cardiovascular and muscular systems are still being discovered and can be assessed non-invasively. The purpose of this dissertation was to examine the non-invasive markers of stretching and yoga on the metabolic, cardiovascular, and muscular systems. A series of three studies were conducted to gain insight into how stretching and yoga influence the body and if there are any potential differences between them.

The first study aimed to determine if stretching would influence fiber type and motor unit recruitment. An acute bout of stretch exercise was performed immediately before a biking exercise session …

Characterizing The Functional Role Of Transcription Factor E In Archaeal Transcription, Madolyn Hofstetter, Michael Bartlett

Student Research Symposium

Archaea and eukaryotes share many homologous proteins and essential mechanisms for survival and DNA replication. It is proposed that Archaea are the ancestors of eukaryotes, suggesting that eukaryotic transcription evolved from the basic archaeal mechanisms. By studying the fundamentals of archaeal transcription, we are in turn understanding more about how eukaryotic transcription functions. Many human diseases are linked to mishaps and mutations in the process of transcribing DNA to mRNA, so it is essential to explore structures and how mutations in these structures affect the essential function of transcription, and if these mutations also play a role in human disease.

Visualization Of Eye Development In Annual Killifish Using Whole-Mount Immunohistochemistry, Carmen Z. Rodriguez, Jason Podrabsky

Student Research Symposium

Vertebrate eye development is highly conserved. Current models have identified the gene networks involved in eye development, but lack the context of extreme environmental conditions that challenge these fundamental programs. Typical vertebrates live for many years while annual killifishes can complete their entire adult lifespan in a matter of weeks. Thus, annual killifishes are a strong model for age-related diseases such as age-related macular degeneration (AMD); an irreversible loss of central vision. Annual killifishes enter embryonic diapause; a period of developmental and metabolic dormancy. These embryos are extremely resistant to environmental stresses such as hypoxia/anoxia that would cause irreparable damage …

Survey Of The Bees And Floral Use At The Leach Botanical Garden, Joshua Page

Student Research Symposium

There have been few studies of the diversity of bee species found in Oregon’s urban areas, and fewer studies that have systematically examined how bee populations change over the seasons. A survey of native bee populations at the Leach Botanical Gardens was conducted to determine what bees were present, how they changed over the season, and what flowers they used. Biweekly over the 2020 and 2021 bee seasons, sections of the garden were sampled in 30 minute equal time intervals, divided among the patches of flowering plants on which bees were observed. Bee specimens were captured with collection vials on …

Mutant Viruses From Hell: Comparing The Capsid Structure Of Vp1 Mutants To The Wild-Type, Jenni Tran, Thejanee Liyanaarachchi, Ignacio De La Higuera, Jono Abshier, Ken Stedman

Student Research Symposium

Viruses can be defined as genetic material tightly packed in a protein coat called the capsid. The spindle-shaped fuselloviruses are found worldwide in hot springs at over 80°C and below pH 4. Sulfolobus spindle-shaped virus 1 (SSV1) is a fusellovirus known to infect the archaea, Saccharolobus solfataricus. This research will provide further insights into the survival of macromolecules, particularly proteins, that are present in extreme environments. To study the interaction of proteins within these extreme environments, we are studying the capsid structure of SSV1. The capsid of SSV1 is composed of two main proteins, VP1 (major) and VP3 (minor). There …

Using Wgcna To Examine Rnaseq Expression Data In The Annual Killifish Austrofundulus Limnaeus, Patrick Clouser, Jason Podrabsky

Student Research Symposium

Annual killifish (Austrofundulus limnaeus) live in ephemeral ponds of Venezuela. They have evolved a unique life history that includes experience embryonic diapause; a period of developmental dormancy and metabolic arrest that supports survival during the dry season. Developing and diapausing embryos are extremely resistant to environmental stresses; for example, they can survive for months without oxygen (anoxia). A. limnaeus’ tolerance to anoxia is an important survival mechanism as they can spend months buried in anoxic soil during development. I hypothesize that this extreme anoxia tolerance is supported by gene expression networks that are different from typical vertebrates. To evaluate this …

An Investigation Of Microbial Symbiosis In Vertebrate Embryonic Diapause Using Austrofundulus Limnaeus As A Model, Jose Camara-Lavadores

Student Research Symposium

Studies emphasizing the crucial dynamic between multicellular organisms and microbes continue to broaden our understanding on how these relationships lead to pathogenicity or mutual benefit. Many studies show that microbes can aid hosts through a variety of mechanisms including: metabolism, neuro development, physical performance, and behavior. In this study we aim to characterize the microbiota of the annual killifish Austrofundulus limnaeus, and investigate the potential role that microbes may have in supporting embryonic diapause. Diapausing embryos can survive extreme environmental stresses such as anoxia, drought, and fluctuating osmolarity and pH. Understanding the potential role that microbes may play in …

Effect Of Eosinophils In Purinergic Receptor P2x3 Expression In Mouse Sensory Neurons, Emily J. Mize

Student Research Symposium

P2X₃ purinoceptors are expressed by airway sensory neurons and activated by extracellular ATP released during periods of cell stress. In asthma, sensitivity to ATP is increased. Airway eosinophilia, which is common in a majority of asthmatics, increases airway sensory nerve density in mice and humans. Whether eosinophils increase neuronal P2X₃ expression in asthma is unknown.

P2X₃ expression was quantified in vagal ganglia from wild-type (WT) mice and transgenic mice with airway eosinophilia (NJ1726), systemic eosinophilia (NJ1638), and eosinophil-deficiency (PHIL) using RNAscope in situ hybridization. Images were obtained using confocal microscopy. The percentage of P2X₃ positive pixels …

How Are Changes In Temperature And Salinity Impacting Intertidal Mussels?, Casey A. Martin

Student Research Symposium

Marine organisms living within the intertidal zones, such as mussels, experience a wide range of environmental conditions every day. Due to Climate change, these environments are transforming in such a way that may impact a mussels ability to successfully reproduce, grow, and live. Previous studies have shown that successful gamete development in freshwater mussels has been linked to temperature and species behavior has been observed to be influenced by salinity levels. In order to test the impact of temperature and salinity on mussel physiology, mussels were exposed to several treatments with varying conditions. Those conditions included a combination of a …

Exploring The Function Of Heart Regeneration In Survival Of Anoxia In Embryos Of The Annual Killifish Austrofundulus Limnaeus, Connor Taylor Kime

Student Research Symposium

Cardiovascular disease is the leading cause of death in the world, half of which is caused by myocardial infarction (MI) or heart attack. Most of the damage resulting from MI is due to oxygen deprivation that leads to cell death. The resulting cardiac scarring due to MI is chronic in humans and is not yet treatable; the only method of restoration is heart transplantation. Other organisms, such as fishes, show great cardiac regenerative capacity. The annual killifish, Austrofundulus limnaeus, can survive for months without oxygen at certain developmental stages. Examining gene expression responses during anoxic exposure and recovery in …

Impacts Of Doliolids On The Marine Microbial Community Off The Oregon Coast, Melissa Steinman, Anne Thompson, Kelly Sutherland

Student Research Symposium

Marine microbes drive global biogeochemical cycling and are critical to sequestering carbon dioxide from the atmosphere to the ocean. Predation on marine microbes by viruses and single-celled microbes is well documented, yet models of microbial mortality with these sources do not account for observations of microbial death. We examined how an understudied group of animals, pelagic tunicates, could feed on marine microbes using an internal mucous mesh. One group of pelagic tunicates, the doliolids, have the potential to reshape marine microbial communities when they bloom. We sampled doliolids off the Oregon Coast and characterized the diversity, taxonomy, and relative abundance …

Mechanisms Of Telomere Maintenance In Trypanosoma Brucei, M A G G. Rabbani

ETD Archive

Telomeres are a nucleoprotein structure at the end of the chromosome and are essential for genome integrity and chromosome stability. Telomere lengths are primarily maintained by a telomerase-mediated pathway but can be maintained by a homologous recombination-mediated pathway. However, detailed mechanisms of telomere maintenance are still unclear in many eukaryotes, including an important human pathogen, Trypanosoma brucei. Telomeres can be elongated by telomerase in T. brucei, a causative agent of fatal sleeping sickness in humans and nagana in cattle. T. brucei evades host immune response by regularly switching its major surface antigen, variant surface glycoprotein (VSG), a process known as …

The Open-Ocean Gulf Of Mexico After Deepwater Horizon: Synthesis Of A Decade Of Research, Tracey Sutton, Rosanna J. Milligan, Kendra Daly, Kevin M. Boswell, April B. Cook, Maëlle Cornic, Tamara Frank, Kaitlin Frasier, Daniel Hahn, Frank Hernandez, John Hildebrand, Chuanmin Hu, Matthew Johnston, Samantha B. Joye, Heather Judkins, Jon A. Moore, Steven A. Murawski, Nina Pruzinsky, John A. Quinlan, Andrew Remsen, Kelly L. Robinson, Isabel C. Romero, Jay R. Rooker, Michael Vecchione, R. J. David Wells

Marine & Environmental Sciences Faculty Articles

The scale of the Deepwater Horizon disaster was and is unprecedented: geographic extent, pollutant amount, countermeasure scope, and of most relevance to this Research Topic issue, range of ecotypes affected. These ecotypes include coastal/nearshore, continental shelf, deep benthic, and open-ocean domains, the last of which is the subject of this synthesis. The open-ocean ecotype comprises ~90% of the volume of the Gulf of Mexico. The exact percentage of this ecotype contaminated with toxins is unknown due to its three-dimensional nature and dynamics, but estimates suggest that the footprint encompassed most of its eastern half. Further, interactions between the water column …

Discovery Of Truffles (Tuber Species) In North Mississippi Pecan Orchards, Scout Hodges

Honors Theses

This thesis describes a study conducted in the years 2019-2022, which aimed to discover a new species of truffle in the state of Mississippi. Tuber lyonii, more commonly referred to as the “pecan truffle,” is an ectomycorrhizal fungus found on the roots of pecan trees in much of the southeastern region of the United States. Though there are many truffles native to the United States, the pecan truffle is one of the few that has been found to have high culinary value. Given the plethora of pecan orchards in the state of Mississippi, I decided to search for evidence …

Characterizing The Roles Of Cannabinoid Receptor 1 & 2 In Zebrafish Behavior, Metabolism, And Seizure-Induced Activity, Kayci Kimmons

Honors Theses

Epileptic disorders like Dravet Syndrome require novel studies to determine the most ideal treatment. New research linking the endocannabinoid system (ECS) to epileptic disorders is arising, but there is still much to be discovered about the function and regulatory impact of the endocannabinoid system and its receptors in epilepsies like Dravet. In this study, knockout models of larval and adult zebrafish (Danio rerio) were used to investigate the roles of cannabinoid receptors 1 & 2 in behavior, brain mitochondrial metabolism, and seizure-induced activity following exposure to THC and CBD. Larval zebrafish which lacked cannabinoid receptor 1 exhibited increased …

Species Diversity And Growth Rates Of Overstory Vegetation Over 25 Years In Response To Physical And Chemical Properties In Long-Term Monitoring Plots At The University Of Mississippi Field Station, Mitchell Tharp

Honors Theses

Overstory vegetation for twenty-two long-term monitoring plots (LTMPs) was sampled from 1996 to 2021 to study the changes in vegetation due to natural and human disturbance. From the fall of 2020 through the fall of 2021, the overstory of the 22 LTMPs was resampled and soil samples were collected from each plot. The circumference at breast height (CBH) and species diversity were recorded and compared to previous years’ data. The objectives of this study were to: (1) document changes in the overstory species of the LTMPs, (2) measure growth rates of surviving trees, (3) survey how elevation and slope impact …

Covalent Modification Of Recombinant Protein With Reactive Thiols, Sawyer Dulaney, Bailey Taylor

Honors Theses

Many diseases cause chronic and painful inflammation in different body systems. One of the front-line drug classes to treat such inflammation is Nonsteroidal Anti-Inflammatory Drugs (NSAIDs). Despite the benefits of oral administration of NSAIDs, there are drawbacks to their long-term usage because they can cause detrimental effects on off-target systems in the body such as the liver, kidney, or the lining of the intestinal tract. An alternative to NSAIDs is the usage of hydrogels for targeted drug delivery. Hydrogels can provide drug delivery in a specific portion of the site of inflammation, thus allowing higher doses of medication to be …

The Effects Of Crowding Agents On The Pka Of Physiologically Stable I-Motif Dna, Courtney Turner

Honors Theses

The overall goal of this thesis was to investigate the environmental conditions that induce i-Motif folding of the DNA strand DUX4L22, a cytosine-rich segment of DNA found in the human genome. Cellular conditions were simulated using sodium cacodylate buffer and multiple weights of polyethylene glycol as a crowding agent. The presence of i-motifs were confirmed by Circular Dichroism spectroscopy. I found that DUX4L22 does form i-motifs under these physiological simulations at both acidic and neutral pHs. DUX4L22 therefore shows potential for use in studies of a wide variety of biotechnological advances, such as regulatory switches in nanomachines or drug-delivery systems.

Individual Beef Cattle Identification Using Muzzle Images And Deep Learning Techniques, Guoming Li, Galen E. Erickson, Yijie Xiong

Department of Animal Science: Faculty Publications

The ability to identify individual animals has gained great interest in beef feedlots to allow for animal tracking and all applications for precision management of individuals. This study assessed the feasibility and performance of a total of 59 deep learning models in identifying individual cattle with muzzle images. The best identification accuracy was 98.7%, and the fastest processing speed was 28.3 ms/image. A dataset containing 268 US feedlot cattle and 4923 muzzle images was published along with this article. This study demonstrates the great potential of using deep learning techniques to identify individual cattle using muzzle images and to support …

Unusual High-Frequency Mechanical Properties Of Polymer-Grafted Nanoparticle Melts, Mayank Jhalaria, Yu Chang, Yucheng Huang, Brian C. Benicewicz, Sanat K. Kumar, George Fytas

Faculty Publications

Brillouin light spectroscopy is used to measure the elastic moduli of spherical polymer-grafted nanoparticle (GNP) melts as a function of chain length at fixed grafting density (0.47  chains/nm²) and nanoparticle radius (8 nm). While the moduli follow a rule of mixtures (Wood’s law) for long chains, they display enhanced elasticity and anomalous dissipation for graft chains <1⁢0⁢0  kDa. GNP melts with long polymers at high 𝜎 have a dry zone near the GNP core, surrounded by a region where the grafts can interpenetrate with chain fragments from adjacent GNPs. We propose that the departures from Wood’s law for short chains are due to the effectively larger silica volume fraction in the region where sound propagates—this is caused by the short, interpenetrated chain fragments being pushed out of the way. We thus conclude that transport mechanisms (of gas, ions, sound, thermal phonons) in GNP melts are radically different if interpenetrated chain segments can be “pushed out of the way” or not. This provides a facile new means for manipulating the properties of these materials.

Effects Of Water Quality On Oyster Health On Georgia Coast, Laurel Henderson

Honors College Theses

ABSTRACT

Oysters are an economically and ecologically important shellfish species found along most coastlines. Along the Georgia coastline, oysters form extensive reefs, and are economically important as a food source. In addition, oysters perform a vital role in maintaining water quality due to their high filtration capacity in coastal waters. Oyster reefs form solid, 3-D substrate that prevent coastline erosion and provide structure for numerous other coastal species to grow, including many commercially and recreationally important species. Water quality factors, such as temperature and salinity, can influence oyster density, condition, and growth, and ultimately affect the services the reefs provide …

Are You Paid For Your Hard Red Winter Wheat Quality? Yes, But Indirectly, Shane Roberts, Kathleen Brooks, Lia Nogueira, Cory Walters

Cornhusker Economics

Hard red winter wheat (HRWW) is the largest wheat class grown in the United States, with approximately 660 million bushels produced in 2020, representing about 36% of total U.S. wheat production (USDA ERS 2021). The world market is an important destination for U.S. HRWW. In 2020, exports of U.S. HRWW represented 52% of U.S. production and total U.S. wheat exports represented 13% of total world exports (USDA ERS 2021). U.S. HRWW differs from other wheat classes based on physical characteristics such as color, kernel hardness and planting season as well as consistency in producing end uses such as pan and …

B-Vitamins Influence The Consumption Of Macronutrients In Honey Bees, Walaa Ahmed Elsayeh, Chelsea N. Cook, Geraldine A. Wright

Biological Sciences Faculty Research and Publications

Insects require dietary sources of B-vitamins, but relatively little is known about whether they regulate B-vitamin intake in the same way they regulate other nutrients. Honey bees meet their B-vitamin requirements mainly from the pollen they collect. Employing the geometric framework for nutrition, we found that honey bees actively regulate their vitamin intake following Bertrand’s rule. We fed bees with a diet of essential amino acids (EAAs) and carbohydrate (C) to identify how the addition of B-vitamins affected the regulation of these macronutrients. In our experiments, honey bees preferred vitamins in concentrations comparable to those found in honey bee food …

Elucidating Human Milk Oligosaccharide Biosynthetic Genes Through Network-Based Multi-Omics Integration, Benjamin P Kellman, Anne Richelle, Jeong-Yeh Yang, Digantkumar Chapla, Austin W T Chiang, Julia A Najera, Chenguang Liang, Annalee Fürst, Bokan Bao, Natalia Koga, Mahmoud A Mohammad, Anders Bech Bruntse, Morey W Haymond, Kelley W Moremen, Lars Bode, Nathan E Lewis

Children’s Nutrition Research Center Staff Publications

Human Milk Oligosaccharides (HMOs) are abundant carbohydrates fundamental to infant health and development. Although these oligosaccharides were discovered more than half a century ago, their biosynthesis in the mammary gland remains largely uncharacterized. Here, we use a systems biology framework that integrates glycan and RNA expression data to construct an HMO biosynthetic network and predict glycosyltransferases involved. To accomplish this, we construct models describing the most likely pathways for the synthesis of the oligosaccharides accounting for >95% of the HMO content in human milk. Through our models, we propose candidate genes for elongation, branching, fucosylation, and sialylation of HMOs. Our …