Life Sciences Commons^™

Trauma Healing With The Neurosequential Model Of Therapy And Bal-A-Vis-X, Becky Johnston

Educational Specialist, 2020-current

Stigma related to childhood trauma is shifting with the help of advancements in the understanding of neurobiology and interventions that are proving to be effective for healing. There are immense costs and consequences for survivors of childhood trauma and their loved ones that were not so long ago considered irrelevant and the notion that kids bounce back from adversity was previously popular in the psychological community (Perry & Szalavitz, 2017). The broad strokes of Dr. Bruce Perry’s clinical intervention model, The Neurosequential Model of Therapy (NMT) describes a trauma-sensitive, sequential approach to changing the stress response within mental health counseling. …

Human Arhgef9 Intellectual Disability Syndrome Is Phenocopied By A Mutation That Disrupts Collybistin Binding To The Gabaa Receptor Α2 Subunit, Dustin J. Hines, April Contreras, Betsua Garcia, Jeffrey S. Barker, Austin J. Boren, Cristelle Moufawad El Achlar, Stephen J. Moss, Rochelle M. Hines

Psychology Faculty Research

Enrichment of collybistin to parvalbumin positive clusters on the soma of pyramidal cells in mouse cortex. Collybistin (green) is strongly enriched at parvalbumin (red) clusters that surround the pyramidal cell soma. Collybistin remains enriched at the parvalbumin clusters on somas in the cortex of Gabra2-1 mice suggesting that collybistin is trafficked to these sites independent of interaction with the α2 subunit of GABAA receptors. For more information, please refer to the article by Hines et al. in this issue.

Implementing Universal Depression Screening In A College Health Clinic, Marilyn J. Grabau

Doctor of Nursing Practice Scholarly Projects

BACKGROUND: College students are at high risk for depression, and this carries a considerable burden. The American College Health Association supports depression screening on college campuses recognizing that early identification leads to better outcomes.

LOCAL PROBLEM: The project site is a public university located in New England. The college campus health center incorporates medical and counseling services. From August 2019 to December of 2019, 67% of students seen in the counseling center described depressed mood as the presenting concern. Currently there is no routine depression screening in place.

METHODS: This QI project implemented universal depression screening at the student health …

Classification Of Eurasian Watermilfoil (Myriophyllum Spicatum) Using Drone-Enabled Multispectral Imagery Analysis, Colin Brooks, Amanda Grimm, Amy Marcarelli, Nicholas Marion, Robert Shuchman, Michael Sayers

Michigan Tech Publications, Part 1

Remote sensing approaches that could identify species of submerged aquatic vegetation (SAV) and measure their extent in lake littoral zones would greatly enhance SAV study and management, especially if these approaches can provide faster or more accurate results than traditional field methods. Remote sensing with multispectral sensors can provide this capability, but SAV identification with this technology must address the challenges of light extinction in aquatic environments where chlorophyll, dissolved organic carbon, and suspended minerals can affect water clarity and the strength of the sensed light signal. Here, we present an uncrewed aerial system (UAS)-enabled methodology to identify the extent …

What Uv Is What You Get, Jaylin Chlosta, Cassidy Mills, Mason Peckar, Nicholas Schaertl

Student Work

Vitamin D deficiency affects people differently and causes multiple health problems. Supplements may help when UV exposure is insufficient to generate Vitamin D in the body.

Investigation Of Dna Variability And Phylogenetic Relationships Of Perlesta (Plecoptera: Perlidae) In Mississippi, James C. Valentine, Mac H. Alford

Master's Theses

The genus Perlesta Banks, 1906 (Plecoptera: Perlidae) consists of 35 species, 33 native to the United States and Canada and two native to China. For over a century these small, brown stonefly adults and freckled yellow nymphs have gone by the name of the type species of the genus, Perlesta placida, but taxonomic work in the genus since 1989 has resulted in the recognition of additional species. These species were mostly recognized and described using morphological characteristics, but two areas that are lacking include (1) linking nymphs to adults and (2) phylogenetic analysis of all species occurring in Mississippi …

Induced Spawning Of Captive Tripletail Lobotes Surinamensis, Nicholas Adams

Master's Theses

The Tripletail, Lobotes surinamensis, is an emerging candidate species for U.S. marine aquaculture. This work aimed to address two bottlenecks for hatchery production of the species by developing a hormonal induction protocol to obtain fertile spawns from captive brooders and a method for sex identification of candidate brooders.

Single pairs selected among a captive-held broodstock conditioned under a natural photothermal cycle were induced with one of five treatments (n = 5 or 6 replicates per treatment). Control (no hormone) and hCG (1,100 IU.kg^-1 for females, 550 IU.kg^-1 for males) pairs did not spawn. Pairs treated with GnRHa …

Reassessment Of Species Boundaries And Phylogenetic Relationships In The Desmodium Ciliare Complex (Fabaceae) Using Morphological And Dna Data, Joshua Wilkinson

Master's Theses

Desmodium (Beggar's ticks) is a generally weedy genus of approximately 280 species in the angiosperm family Fabaceae (Subfamily Papilionoideae, Tribe Desmodieae) characterized by indehiscent loments constricted into segments. Within the Southeastern U.S., the Desmodium ciliare group is one of two Desmodium species complexes that have been historically difficult, with much argument about the limits of species and the role of hybridization. There are three commonly recognized species within the D. ciliare group, D. ciliare (Muhl. ex Willd.) DC., Desmodium marilandicum (L.) DC., and D. obtusum (Muhl. ex Willd.) DC., with two supposed hybrids in addition to the …

Physiological Costs Of Total Cholesterol In European Starlings Across An Urban To Rural Gradient, Courtney Linkous

Master of Science in Integrative Biology Theses

Urban areas—characterized by high human densities and associated buildings and impermeable surfaces—are increasing globally and represents a leading threat to wildlife that is drastically altering the natural resources they are accustomed to. Prior studies suggest that living in urban habitats can cause wildlife to show increased cholesterol levels; in biomedical research, elevated cholesterol is linked to disease, but the consequence of elevated cholesterol in wildlife remains unclear. We measured total cholesterol in European starlings (Sturnus vulgaris)—an urban adapted species—across an urban and a rural site. We ask: (1) do urban starlings have elevated cholesterol, (2) does elevated cholesterol …

Just Cut It Out, Jack Barbosa, Ashley Curcio, Emiliana Martinez-Nobrega, Matthew Robledo

Student Work

Why should we care about trees? Social, Economic, and Environmental benefits.

The Effect Of Acute Caffeine Withdrawal On Exercise Performance In Habitual Caffeine Users, Timothy D. Griest

Masters Theses, 2020-current

Purpose: The purpose of this study was to investigate how exercise performance is impacted by acute withdrawal from caffeine (CAF) in habitual users and if CAF ingestion will reverse the negative impact of withdrawal or have a net positive benefit. Methods: Ten recreational cyclists (age 39.1 ± 14.9 y; VO_2max 54.2 ± 6.2 mL/kg/min) who were habitual CAF consumers (394 ± 146 mg/d) completed four trials, each consisting of peak isokinetic torque testing and a 10-km time trial (TT). On each trial day, subjects consumed either 1.5 mg/kg CAF to prevent withdrawal or placebo (PLA) 8 hours before their …

Urban Habitats Impact The Song And Reproductive Success Of A Migratory Bird, The Gray Catbird (Dumetella Carolinensis), Sasha Ewing

Masters Theses, 2020-current

Urban environments are relatively recent on an evolutionary timescale, and, as such, they create novel selection pressures that may influence fitness and mate choice. Many ecologically significant factors change with increasing levels of urbanization, such as increasing anthropogenic noise, increasing impervious surface, and decreasing forest cover. There is growing empirical evidence that anthropogenic noise affects features of birdsong and, separately, that avian nesting success varies across an urban gradient, but rarely do studies examine both – a necessary step to elucidate the evolutionary potential of these novel pressures. I investigated song features, body morphometrics, and reproductive success in male gray …

The Impact Of Iaa And Tryptophan On Microcystis Bloom Community Dynamics, Hunter R. Baylous

Masters Theses, 2020-current

Microcystis aeruginosa is a freshwater cyanobacterial species that degrades freshwater and brackish ecosystems due to its capacity to form toxic cyanobacterial harmful algal blooms (cyanoHABs). There both abiotic and the biotic factors that exacerbate the presence of cyanoHABs. Current research is looking at the microbial interactions that occur between microbes and phytoplankton. Microbes interact with cyanobacteria in the phycosphere, where nutrients are exchanged between the two. Understanding the chemical currencies exchanged can help to show interactions that are beneficial for cyanoHAB formation A key player in the growth promotion of cyanoHABs is hypothesized to be auxins, which are synthesized by …

The Synthesis Of 3-Formyltyrosine For The Purpose Of In Virto Fluorescent Visualization Of Microtubules, Angelina Bonacasa

Undergraduate Honors Theses

Microtubules and the desire to visualize them has been of utmost importance in understanding the behavior of the cytoskeleton. Even further, scientists are using this knowledge to elucidate cancerous activities as they occur within cells. In order to acquire this knowledge, the following methods have been established: the expression of tubulin after it has been fused with a fluorescent protein, the insertion of tubulin that has been exogenously labeled, and the use of fluorescent-probe conjugated microtubule ligands, such as paclitaxel. These methods, however, tend to distort the protein’s native function, thereby preventing an accurate analysis. This concern drives the need …

The Effect Of Creatine Supplementation On Body Composition And Total Body Water Measured By Multi-Frequency Bioelectrical Impedance, Emily A. Buck

Masters Theses, 2020-current

Background: Acute fluid ingestion causes an increase in estimated body fat percentage (BF%) measurements by single frequency (SF-BIA) and multi-frequency bioelectrical impedance (MF-BIA). However, it is unknown if MF-BIA accurately measures total body water (TBW) and BF% after chronic fluid retention. Creatine supplementation causes fluid retention, and resultant increases in TBW and body mass. Research is needed to determine if MF-BIA is capable of detecting fluid retention secondary to creatine supplementation. Methods: 13 male and 14 female subjects (18-22 y) completed one week of creatine monohydrate or maltodextrin supplementation at a dose of 0.3 g/kg body weight. Subjects completed pre-supplementation …

The Effect Of Dietary Nitrate Supplementation On Physical And Cognitive Performance During Load Carriage In Military Cadets, Nicholas C. Bordonie

Masters Theses, 2020-current

Purpose: The purpose of this study was to determine the effects of dietary nitrate supplementation on physical and cognitive performance during exercise conducted under heavy pack load in military cadets. Methods: Ten college-aged males (VO_2max 56.2 ± 3.7 ml/kg/min) consumed 140mL/d of concentrated beetroot juice (BRJ; containing 12.8 mmol of dietary nitrate) or a placebo (PL; flavor, color, energy, and texture-matched with no dietary nitrate) for six days preceding an exercise trial. The trial consisted of 45 min of constant-load exercise on a treadmill at 4.83 km/h and 1.5% grade, followed immediately by a 1.6-km time-trial completed at maximal …

Pilfering Passerines? Inter-Class Competition: A New Direction In Foraging Ecology, Shannon Gillen

Masters Theses, 2020-current

Life in the epigeal layer below leaf litterfall often goes unexplored. Shrews (Soricidae, 1910) have foraged in this microhabitat for millions of years, carving out an existence in harsh habitats around the world, ranging from the tropics to subarctic boreal tundra. Two shrew species that inhabit the Shenandoah Valley of Virginia, the least shrew (Cryptotis parva) and northern short-tailed shrew (Blarina brevicauda), were the focus of this study. Shrews make epigeal foraging trails while they continually forage for food, and caching is a way to save this food for times of low and sporadic food availability. …

The Effect Of Intensity And Duration Of Verification Stages On The Efficacy Of Vo2max Testing, Andrew Foster

Masters Theses, 2020-current

Purpose: To determine if changing the length of the recovery stage influences the effectiveness, or ability to confirm maximal oxygen consumption (VO_2max), of the verification phase and if the initial intensity of the stage has an impact on its effectiveness. Methods: 27 subjects (20 males and 7 females) performed four separate VO_2max tests separated by at least 48 hours. For each initial graded exercise test, starting speed was 3.0 mph and increased by 0.5 mph every minute until 6.0 mph was reached. After this point, elevation was increased by 3.0% every minute until volitional exhaustion. VO_{2 …}

Does A Bout Of Acute Resistance Exercise Attenuate The Post-Prandial Lipemic Response To A High Fat Meal In 50-80 Year-Old Adults, Summer Simulcik

Masters Theses, 2020-current

Background: A single high-fat meal (HFM) is known to elevate blood lipid levels above what is normal, resulting in postprandial lipemia (PPL). Chronic hyperlipemia has been associated with increased risk for cardiovascular disease (CVD) and type-2 diabetes mellitus (T2DM) development. Current physical activity (PA) guidelines recommend at least one set of 8-12 repetitions of each exercise to attenuate this risk. Adults older than 50 (OF) years (y) are at an increased risk for cardiometabolic disease compared to younger adults (YA). Aerobic exercise (AE) performed in the pre-prandial period has been observed to attenuate PPL in both OF and YA. However, …

Assessing Red Spruce (Picea Rubens) Restoration Potential Under Current And Future Predicted Climate Change In Virginia, Christian Brown

Masters Theses, 2020-current

Global climate change threatens many species across the planet. High-elevation species, such as red spruce (Picea rubens), face significant and immediate threats from climate change. Red spruce has faced anthropogenic disturbances for over a century and is only recently beginning to regenerate across its range, making it an ideal restoration candidate. Ecological niche modeling has become a common method of identifying the suitable habitat of a species, providing vital information to land managers carrying out restoration efforts. In this study ecological niche models were used in a novel way, predicting distribution and habitat suitability separately to determine the …

Urbanization Drives Changes In Avian Communities, Which Are Reflected In The Song Of A Vocal Mimic, Jaclyn Tolchin

Masters Theses, 2020-current

Urban landscapes present problems for wildlife including highly modified habitat, anthropogenic noise pollution, and competition for suitable habitat. These novel selection pressures can filter species present in urban habitats or result in changes to behavior. Recent studies show that some bird species sing differently (louder, higher pitch, faster) in noisier, urban areas compared to quieter, more rural areas, and that anthropogenic noise alone can exclude species. I investigated how the level of urbanization affects 1) bird species composition, and 2) the assemblage of species that a songbird mimics from the local bird community. I studied gray catbirds, Dumetella carolinensis, across …

Identifying Key Stream Restoration Variables In An Agriculturally Impaired Chesapeake Bay Watershed, Julia Portmann

Masters Theses, 2020-current

The Chesapeake Bay watershed spans several states, supports diverse ecosystems, and is crucial to local economies. However, agricultural practices in this region impair water quality. The Smith Creek watershed, within the Shenandoah Valley, was designated a showcase watershed in 2010 by the United States Department of Agriculture to demonstrate the efficacy of implementing restoration projects. We sampled fifteen farms ranging from unrestored to thirty-six years since restoration. At each site, we conducted a kick-net survey for macroinvertebrates, measured canopy cover, algal density, substrate size, and bank height and angle. We identified macroinvertebrates to family and calculated the Chesapeake Basin-wide Index …

The Impact Of High- Vs. Low-Load Resistance Training On Measures Of Muscle Activation, Strength, Body Composition, And Hormonal Markers, Marissa Laina Bello

Theses and Dissertations

Resistance training has shifted towards a high- vs low-load training approach. Heavier loads are suggested to maximally recruit motor units and optimize strength adaptations, whereas lower loads stimulate hypertrophy. However, a majority of the research has not used a true strength range when assessing load. Therefore, the purpose of this investigation was to examine and determine significant differences in strength, body composition, and hormonal markers over nine weeks of high- or low-load resistance training. Secondary purposes of the current investigation were to assess and quantify training load for resistance training using sEMG sensor-embedded compression shorts. 17 recreationally-trained males were randomized …

Factors Associated With Covid-19 Death In The United States: Cohort Study, Uan-I Chen, Hua Xu, Trudy Millard Krause, Raymond Greenberg, Xiao Dong, Xiaoqian Jiang

Faculty, Staff and Student Publications

BACKGROUND: Since the initial COVID-19 cases were identified in the United States in February 2020, the United States has experienced a high incidence of the disease. Understanding the risk factors for severe outcomes identifies the most vulnerable populations and helps in decision-making.

OBJECTIVE: This study aims to assess the factors associated with COVID-19-related deaths from a large, national, individual-level data set.

METHODS: A cohort study was conducted using data from the Optum de-identified COVID-19 electronic health record (EHR) data set; 1,271,033 adult participants were observed from February 1, 2020, to August 31, 2020, until their deaths due to COVID-19, deaths …

Characterization Of A Helicobacter Pylori Small Rna By Rt-Pcr, Roxanne Mcpeck, Andrea Castillo

2022 Symposium

Helicobacter pylori, a bacterial gastric pathogen infecting approximately 50% of the human population, produces gastritis, ulcers, and gastric cancers. Colonizing the inhospitable and fluctuating environment in the stomach requires tight genetic control. However, H. pylori lacks many genetic regulatory elements present in other bacteria to control gene expression. Instead, over 200 small RNAs (sRNAs; noncoding RNAs shorter than 300 nucleotides) have been found in this bacterium, but few have been fully characterized. Of those, many are antisense to virulence genes. Characterizing these sRNAs is important in understanding the mechanisms of molecular genetics and potentially supporting medical management of this …

Does Manuka Honey Induce Antibiotic Resistant Bacterial Persisters And Viable But Non-Culturable Subpopulations?, Bryn Tennyson

2022 Symposium

Long-term antibiotic use has allowed microbes to develop resistance and cause chronic infectious diseases that were previously treatable. Resistance is phenomenon in which bacteria survive treatment with a concentration of antibiotics that was once lethal. Bacteria resist antibiotic treatment by genetic change or initiating states of dormancy called persisters or Viable but Non-Culturable (VBNCs) cells. These subpopulations of antibiotic resistant persisters and VBNCs increase with exposure to stresses, including antibiotic treatment, and are a major cause of reoccurring infections that result in significant morbidity and mortality. Manuka honey (MH), a well-known alternative broad-spectrum antimicrobial, is a promising treatment for cutaneous …

Development And Validation Of High-Resolution Melting Assays For The Detection Of Potentially Virulent Strains Of Escherichia Coli O103 And O121, Frank J. Velez, Joseph M. Bosilevac, Sabine Delannoy, Patrick Fach, Ravinder Nagpal, Prashant Singh

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Virulent strains of Shiga toxin-producing Escherichia coli (STEC) serogroups O103 and O121 are considered adulterants in beef. Two high-resolution melting (HRM) real-time PCR assays were standardized for the specific detection and discrimination of potentially virulent and avirulent strains of E. coli O103 and O121. The O103 HRM assay offered the possibility to distinguish clearly STEC O103:H2 from STEC O103:H25. The two standardized assays were extensively validated using 215 pure culture strains, laboratory inoculated food samples, and naturally contaminated beef (n = 84) and pork (n = 84) enrichments collected from the red meat surveillance program. Both HRM assays showed 100% …

Combining Genomic And Epidemiological Data To Compare The Transmissibility Of Sars-Cov-2 Variants Alpha And Iota., Mary E Petrone, Jessica E Rothman, Mallery I Breban, Isabel M Ott, Alexis Russell, Erica Lasek-Nesselquist, Hamada Badr, Kevin Kelly, Gregory Omerza, Nicholas Renzette, Anne E Watkins, Chaney C Kalinich, Tara Alpert, Anderson F Brito, Rebecca Earnest, Irina R Tikhonova, Christopher Castaldi, John P Kelly, Matthew Shudt, Jonathan Plitnick, Erasmus Schneider, Steven Murphy, Caleb Neal, Eva Laszlo, Ahmad Altajar, Claire Pearson, Anthony Muyombwe, Randy Downing, Jafar Razeq, Linda Niccolai, Madeline S Wilson, Margaret L Anderson, Jianhui Wang, Chen Liu, Pei Hui, Shrikant Mane, Bradford P Taylor, William P Hanage, Marie L Landry, David R Peaper, Kaya Bilguvar, Joseph R Fauver, Chantal B F Vogels, Lauren M Gardner, Virginia E Pitzer, Kirsten St George, Mark D Adams, Nathan D Grubaugh

Faculty Research 2022

SARS-CoV-2 variants shaped the second year of the COVID-19 pandemic and the discourse around effective control measures. Evaluating the threat posed by a new variant is essential for adapting response efforts when community transmission is detected. In this study, we compare the dynamics of two variants, Alpha and Iota, by integrating genomic surveillance data to estimate the effective reproduction number (R_t) of the variants. We use Connecticut, United States, in which Alpha and Iota co-circulated in 2021. We find that the R_t of these variants were up to 50% larger than that of other variants. We then …

Using Lidar To Estimate Carbon Sequestration Of Evergreen Trees At Eastern Washington University (Ewu) Campus, Cheney, Washington, Kristy A. Snyder

2022 Symposium

EWU contains a variety of deciduous and evergreen trees across its campus, providing several benefits. However, no comprehensive record exists of the total number, location, species, or ages of these trees. This knowledge can inform facilities of proper care for individual trees and can be used to estimate carbon sequestration on campus. Traditional on-the-ground methods for assessing trees require tree cores or clinometers, making trees susceptible to pests or disease and leading to inaccurate results. Remote sensing using lidar data is a noninvasive, more precise method to measure tree height and subsequently assess tree age. This poster explores using point …

Clock-Linked Genes Underlie Seasonal Migratory Timing In A Diurnal Raptor, Christen M. Bossu, Julie A. Heath, Gregory S. Kaltenecker, Barbara Helm, Kristen C. Ruegg

Biology Faculty Publications and Presentations

Seasonal migration is a dynamic natural phenomenon that allows organisms to exploit favourable habitats across the annual cycle. While the morphological, physiological and behavioural changes associated with migratory behaviour are well characterized, the genetic basis of migration and its link to endogenous biological time-keeping pathways are poorly understood. Historically, genome-wide research has focused on genes of large effect, whereas many genes of small effect may work together to regulate complex traits like migratory behaviour. Here, we explicitly relax stringent outlier detection thresholds and, as a result, discover how multiple biological time-keeping genes are important to migratory timing in an iconic …