Life Sciences Commons^™

Dj-1 Is Not A Deglycase And Makes A Modest Contribution To Cellular Defense Against Methylglyoxal Damage In Neurons, Melissa Conti Mazza, Sarah C. Shuck, Jiusheng Lin, Michael Moxley, John Termini, Mark R. Cookson, Mark A. Wilson

Department of Biochemistry: Faculty Publications

Human DJ-1 is a cytoprotective protein whose absence causes Parkinson's disease and is also associated with other diseases. DJ-1 has an established role as a redox-regulated protein that defends against oxidative stress and mitochondrial dysfunction. Multiple studies have suggested that DJ-1 is also a protein/nucleic acid deglycase that plays a key role in the repair of glycation damage caused by methylglyoxal (MG), a reactive α-keto aldehyde formed by central metabolism. Contradictory reports suggest that DJ-1 is a glyoxalase but not a deglycase and does not play a major role in glycation defense. Resolving this issue is important for understanding how …

Hiding Behind The Title: Bridging The Gap Between Identity And Leadership Through Neuroscience, Emotional Intelligence And Ethical Management, Alicia S. Huddleston

College of Education Theses and Dissertations

Leadership is the key determinant in the success of any organization, and leaders have an immeasurable impact on the lives and performances of those they have been entrusted to develop, support and lead. School principals are an integral part of the leadership tapestry within society and many people have been impacted by a school principal. Often enough, there are individuals who lead with or behind their title versus understanding first who they are intrinsically, and then working through those dynamics to effectively lead their own lives. When leaders are able to do this, then and only then are they equipped …

Ciliogenesis Mechanisms Mediated By Pak2-Arl13b Signaling In Brain Endothelial Cells Is Responsible For Vascular Stability, Karthikeyan Thirugnanam, Shubhangi Prabhudesai, Emma Van Why, Amy Pan, Ankan Gupta, Koji Foreman, Rahima Zennadi, Kevin R. Rarick, Surya M. Nauli, Sean P. Palacek, Ramani Ramchandran

Pharmacy Faculty Articles and Research

In the developing vasculature, cilia, microtubule-based organelles that project from the apical surface of endothelial cells (ECs), have been identified to function cell autonomously to promote vascular integrity and prevent hemorrhage. To date, the underlying mechanisms of endothelial cilia formation (ciliogenesis) are not fully understood. Understanding these mechanisms is likely to open new avenues for targeting EC-cilia to promote vascular stability. Here, we hypothesized that brain ECs ciliogenesis and the underlying mechanisms that control this process are critical for brain vascular stability. To investigate this hypothesis, we utilized multiple approaches including developmental zebrafish model system and primary cell culture systems. …

Genes Involved In Feed Efficiency Identified In A Meta-Analysis Of Rumen Tissue From Two Populations Of Beef Steers, Amanda K. Lindholm-Perry, Allison M. Meyer, Rebecca J. Kern-Lunbery, Hannah C. Cunningham-Hollinger, Taran H. Funk, Brittney N. Keel

Roman L. Hruska U.S. Meat Animal Research Center: Reports

In cattle, the rumen is an important site for the absorption of feed by-products released by bacterial fermentation, and variation in ruminal function plays a role in cattle feed efficiency. Studies evaluating gene expression in the rumen tissue have been performed prior to this. However, validating the expression of genes identified in additional cattle populations has been challenging. The purpose of this study was to perform a meta-analysis of the ruminal transcriptome of two unrelated populations of animals to identify genes that are involved in feed efficiency across populations. RNAseq data from animals with high and low residual feed intake …

Fluorescent Calixarene-Schiff As A Nanovehicle With Biomedical Purposes, José Antonio Antonio Lebrón, Manuel López-López, María Luisa Moyá, Mary Deasy, Ana Muñoz-Wic, Clara Beatriz García-Calderón, Iván Valle Rosado, Pilar López Cornejo, Eva Bernal Pérez, Francisco José Ostos Marcos

Articles

Gene therapy is a technique that is currently under expansion and development. Recent advances in genetic medicine have paved the way for a broader range of therapies and laid the groundwork for next-generation technologies. A terminally substituted difluorene-diester Schiff Base calix[4]arene has been studied in this work as possible nanovector to be used in gene therapy. Changes to luminescent behavior of the calixarene macrocycle are reported in the presence of ct-DNA. The calixarene macrocycle interacts with calf thymus DNA (ct-DNA), generating changes in its conformation. Partial double-strand denaturation is induced at low concentrations of the calixarene, resulting in compaction of …

Characterizing Endogenous Dicer Products To Unravel Novel Rnai Biogenesis Pathways, Jacob Oche Peter

Dissertations

ABSTRACT

RNA interference (RNAi) is a pervasive gene regulatory mechanism in eukaryotes based on the action of multiple classes of small RNA (sRNA). Exploiting RNAi pathways in non-model systems have great potential for creating potent RNAi technologies. Here, we accessed RNAi-mediated control of gene expression in the two-spotted spider mite, Tetranychus urticae (T. urticae) using engineered dsRNA designed to modulate the host RNAi pathway and increase RNAi efficacy. Analysis of Dicer (Dcr) generated fragments revealed how exogenous RNAs access the host RNAi pathway in this animal, opening avenues for designing RNAi technology for their control. Further, some organisms …

Responses Of Nutrient Resorption To Human Disturbances In Phoebe Bournei Forests, Dehuang Zhu, Suhong Peng, Jinyan Wang, Dafeng Hui

Biology Faculty Research

Nutrient resorption plays an important role in the nutrient conservation of plants and ecosystem nutrient cycling. Although community succession and nutrient addition could regulate plant nutrient resorption, how resorptions of foliar nutrients vary with human disturbances remains unclear. With the economic development, Phoebe bournei forests (PF) have suffered varying degrees of human disturbances in China. In this study, the leaf nutrient resorption efficiency (RE) of the PF under two disturbances (i.e., severe and mild disturbances) were investigated. Results showed that the phosphorus (P) contents of green leaf, senesced leaf, and soil were low under both disturbances, reflecting that the PF …

Book Review – Lean Impact: How To Innovate For Radically Greater Social Good Review & Implications For Engaged Programming, M.C. "Molly" Immendorf

Journal of Human Sciences and Extension

The book “Lean Impact: How to Innovate for Radically Greater Social Good” outlines ways to infuse innovation techniques learned from the private start-up sector into sectors traditionally working to make a societal change that may be funded through governmental agencies and grants. These techniques are also applicable to the Land-Grant Cooperative Extension System (CES) through combining them with engaged programming practices and CES best practices and the tools and methods of the Extension Foundation’s Impact Collaborative in urban programs and beyond.

Amino Acids As Alternatives To Emulsifying Salts In Processed Cheese Analogues, Jeremy Thomas Pack

Theses and Dissertations

Background: Manufacturers have used emulsifying salts universally in the processed cheese industry since James L. Kraft patented the first processed cheese in 1916. My objective was to find alternative ingredients to replace emulsifying salts in processed cheese formulations; the product would follow "clean-labeling" trends and lower the final formulation's sodium content. Materials and Methods: My experiments followed conventional processed cheese formulations to create experimental batches, which were compared to positive and negative controls for significance. Textural, rheological, and melting tests evaluated objective cheese parameters. We observed subjective sensory properties through qualitative descriptive analysis, consumer acceptance panels, and focus groups. Results: …

The Regulation Of Atg9a-Mediated Aggrephagy By An Ulk1-Independent Atg13-Atg101 Complex, Joshua Youngs

Undergraduate Honors Theses

Aggrephagy, a type of autophagy, is an essential cellular process by which protein aggregates are collected and broken down in the lysosome. Protein aggregates are implicated in several diseases including Alzheimer’s disease, diabetes, and cancer. Here, we investigate the ATG13-ATG101 protein complex, a sub-complex of the canonical ULK1 complex whose regulatory role in aggrephagy is not completely understood. We also develop a protein fragment complementation (PFC) assay using the biotin ligase TurboID to study the functions of the ATG13-ATG101 complex with increased specificity. We demonstrate that ATG13 is required for optimal degradation of p62-ubiquitin condensates. We also show that a …

Accessibility And Inclusion As An Approach To Enhancing Local Extension Programs, S. Dee Jepsen, Laura Akgerman, Karen Funkenbusch, Jessie Calero, Heather Kelejian

Journal of Human Sciences and Extension

Providing accessible learning opportunities and inclusive programs are critical to Extension’s mission. Creating inclusive environments is more than consideration for individuals’ personal identity. Using principles of Inclusion, Diversity, Equity, and Accessibility (IDEA) is an approach to intentionally build community and create new opportunities for education and growth. The Americans with Disabilities Act is a federal law requiring businesses and events to be accessible to individuals with disabilities. The Rehabilitation Act of 1973 states, “no qualified individual with a disability may be discriminated against in any program or activity receiving federal financial assistance.” The legislation applies to Extension buildings, programs, and …

Full Issue, Volume 10, Number 1, Donna J. Peterson, Scott Cummings

Journal of Human Sciences and Extension

No abstract provided.

“I’M Going To Live My Life Freely”: Authenticity As An Indicator Of Belonging Among Urban Latinx Lgbtq+ Youth, Maru Gonzalez, Bianka M. Reese, Tania Connaughton-Espino

Journal of Human Sciences and Extension

While there is a growing body of scholarship on the experiences of LGBTQ+ youth in school and community settings, less is known about Latinx LGBTQ+ youth specifically. In response, this phenomenological study examined the experiences of eight Latinx LGBTQ+ youth relative to school and community belonging, with a specific focus on urban environments, using intersectionality and minority stress frameworks, and Lee and Robbins’ operational definition of belongingness. Three overarching themes emerged from the data: (a) navigating challenges, (b) the importance of an inclusive climate, and (c) thriving through adversity. Further, authenticity was identified as an additional indicator of belonging among …

Documentary Review – Two Roles In Urban Community Revitalization: Julian Price The Philanthropist And Cooperative Extension, Susan A. Kelly

Journal of Human Sciences and Extension

Julian Price used his inherited wealth to revitalize downtown Asheville, North Carolina. This documentary review reflects on his passion for community, and the concepts he believed would bring the city back from decay. Some of those same community development practices are successfully used across the country by Extension professionals to assist urban communities in revitalization. Examples of Extension programs and techniques are provided throughout this review about an inspiring documentary about a man looking for purpose and finding it by transforming a troubled downtown with his generosity.

Compensatory Phenolic Induction Dynamics In Aspen After Aphid Infestation, Rajarshi Kumar Gaur, Ilka Nacif De Abreu, Benedicte Riber Albrectsen

Aspen Bibliography

Condensed tannins (CTs) are polyphenolics and part of the total phenolic (TP) pool that shape resistance in aspen (Populus tremula). CTs are negatively associated with pathogens, but their resistance properties against herbivores are less understood. CTs shape resistance to pathogens and chewing herbivores and could also shape resistance to aphids. Being chemical pools that are highly variable it can further be questioned whether CT-shaped resistance is better described by constitutive levels, by the induced response potential, or by both. Here, aspen genotypes were propagated and selected to represent a range of inherent abilities to produce and store foliar …

Overexpression Of Foxf2 In Prostate Cancer Cells Causes An Increase In Cancer Cell Apoptosis And A Decrease In Proliferation, Vaughn Poon

Honors Projects

FOXF2 is a transcription factor that plays a crucial role in organ development, and recent studies have shown that it suppresses tumor growth and progression in mouse prostate models by attenuating the cancer-associated fibroblasts (CAF) phenotype and transcriptionally downregulating Cxcl5. However, the effects of FOXF2 overexpression in prostate cancer cells have not been extensively studied. Here, we investigate the impact of FOXF2 overexpression in prostate cancer cells and demonstrate that it leads to a significant increase in cell apoptosis and a decrease in proliferation. These findings suggest that FOXF2 may have potential as an immunotherapy drug target for prostate …

Challenges Inherent To The Sequencing And Quantification Of Short Non-Coding Rnas And How To Address Them, Justin Gumas, Yohei Kirino

Computational Medicine Center Faculty Papers

No abstract provided.

A Study On Plant-Derived Small Proteins And Peptides With Antiyeast Activity And Preliminary Investigations In Food Materials, Giulia Mignone

Theses

The discovery of novel antimicrobial molecules would benefit considerably different biotechnological fields, including medicine, agriculture and the food industry, on which this thesis work focuses. Specifically, here are presented fundamental studies on plant-derived antimicrobial proteins for the reduction of food spoilage caused by yeast microorganisms. Small proteins and peptides from the plant innate immune system possess several characteristics which could make them ideal candidates for a novel and natural antispoilage agent. The first Chapter reviews proteins families associated with the plant innate immune system that exhibit antiyeast activity and low molecular weight (<30 kDa), focusing on their structure and antiyeast mode of action. Chapter 2 investigates two peptides, Pn-AMP1 and Pn-AMP2 belonging to the hevein-type peptides family; peptides were synthetically generated using their native amino acid sequence. Synthetic Pn-AMP1 showed fast action activity against common food spoilage yeasts, it resulted potentially safe for consumption, and it was successfully incorporated in UHT milk and Fanta Orange, where it contained the growth of Kluyveromyces lactis and Zygosaccharomyces bailii, respectively. A different family of antimicrobial plant proteins (2S albumins) was studied in Chapters 3, 4, 5 and 6. Chapter 3 describes the selective extraction of an antiyeast protein (labelled WMS1 in this thesis work) of ~14 kDa from white mustard (Brassica hirta) seeds. The protein was purified in a relatively high yield, using a simple protocol that included only one chromatographic method step. In Chapter 4, bioinformatics tools and wet-lab techniques are used to study the structure and classify the antiyeast protein. The WMS1 antiyeast protein was identified as an isoform of the Napin protein Allergen Sin a 1 belonging to the 2S albumins family, characterised by high structural stability which donates to the protein resistance heat treatment and proteolytic degradation. Chapter 5 describes the antimicrobial spectrum of WMS1 , its antiyeast mechanism of action and its validity as preservative agent. Although WMS1 resulted potentially safe for consumption and inhibited the growth of Z. bailii in several beverage systems, its biotechnological potential is limited by its allergic properties. In Chapter 6, the protein WMS1 was cloned in a pET28a-MBP vector and expressed in an E. coli BL21 system. Interestedly, recombinant WMS1 and native WMS1 showed similar activity against the yeast Z. bailii. Thesis abstract 2 Chapter 7 includes a protocol for the rational design of ultra-short antimicrobial peptides. Since ultra-short antimicrobial sequences can be found within the sequence of any larger protein, the protocol was applied using WMS1 primary sequence, and seven ultra-short SinA-AMPs were chemically generated. Among the various SinA-AMPs, the peptide labelled SinA-pepIII had in vitro activity against several common yeast spoilage strains via membrane permeabilisation and it could be employed as a food preservative as it was pH- and heat- stable, potentially safe in terms of human consumption and it prevented the growth of Z. bailii in cranberry juice. The results obtained in this work offer new perspectives in the research for novel and clean-label food preservatives to fight the food waste phenomenon.

Examining Limbic Sexual Dimorphisms In Schizophrenia, Kennedy S. Madrid

Undergraduate Honors Theses

Schizophrenia is a mental disorder that affects a significant number of individuals in the United States and can have numerous different symptoms. Recently, interest in the differences between the neuroanatomy of individuals with schizophrenia and individuals without schizophrenia has emerged, specifically the sexual dimorphism in individuals with schizophrenia. This study aimed to gain a better understanding of the sexual dimorphisms of two structures in the limbic system: the hippocampus and amygdala. Data was harmonized and analyzed from two datasets to determine the sexual dimorphic factor of these structures in healthy controls and individuals with schizophrenia. Demographic features were also taken …

Borders: A Story Of Political Imagination, Miriam Ticktin

Publications and Research

This article traces three different political imaginaries about borders, suggesting that the dominant imaginary—the one of border walls, driven by a fear of invasion—is only one way to live in the world. The goal is to make space in our political imaginations to rethink how we live together, including thinking beyond nation-states as containers that keep people in or out. By first showing how the vision of invasion is built and maintained with intersecting transnational technologies and ideologies, I open the way to thinking otherwise. Second, I trace the counterpolitics of borders developed by artists and activists, resisting borders and …

Intra-Axonal Translation Of Khsrp Mrna Slows Axon Regeneration By Destabilizing Localized Mrnas, Priyanka Patel, Courtney N. Buchanan, Matthew D. Zdradzinski, Pabitra K. Sahoo, Amar N. Kar, Seung Joon Lee, Lauren S. Vaughn, Anatoly Urisman, Juan Oses-Prieto, Michela Dell'orco, Devon E. Cassidy, Irene Dalla Costa, Sharmina Miller, Elizabeth Thames, Terika P. Smith, Alma L. Burlingame, Nora Perrone-Bizzozero, Jeffery L. Twiss

Faculty Publications

Axonally synthesized proteins support nerve regeneration through retrograde signaling and local growth mechanisms. RNA binding proteins (RBP) are needed for this and other aspects of post-transcriptional regulation of neuronal mRNAs, but only a limited number of axonal RBPs are known. We used targeted proteomics to profile RBPs in peripheral nerve axons. We detected 76 proteins with reported RNA binding activity in axoplasm, and levels of several change with axon injury and regeneration. RBPs with altered levels include KHSRP that decreases neurite outgrowth in developing CNS neurons. Axonal KHSRP levels rapidly increase after injury remaining elevated up to 28 days post …

Foreword, Mary A. Ruemenapp, Katherine Williams

Journal of Human Sciences and Extension

No abstract provided.

Extension Engagement With Urban Communities: Editors’ Introduction To The Urban-Themed Issue Of Jhse, Julie Fox, Donna J. Peterson, Scott R. Cummings

Journal of Human Sciences and Extension

This introductory article for the special issue of the Journal of Human Sciences and Extension (JHSE) highlights respectful Extension research and engagement with dynamic urban communities. Like the 2017 urban-themed issue of JHSE, the intent is not to diminish the importance of rural and suburban Extension engagement but to better understand unique experiences of Extension in urban communities. The 35 contributing authors represent a range of geographic and programmatic viewpoints. Insights shared

• demonstrate that diversity in our cities is multidimensional;
• reinforce the importance of culturally relevant engagement;
• address issues of access, equity, inclusion, and a sense of belonging;
• highlight the …

Visualizing Diversity: Spatial Data As A Resource Enabling Extension To Better Engage Communities, Justin Krohn, Jacqueline Davis-Manigaulte, Christopher Fulcher, Jennifer Sarah Tiffany

Journal of Human Sciences and Extension

Effective Extension programming relies on engaging people of all races, ethnicities, and cultures. Extension educators sometimes struggle with how best to engage communities that are not “traditional” program audiences. Centering data visualization on the strength of Black, Latino/Hispanic, Asian, Native American, and other potentially marginalized communities can assist Extension’s work to engage diverse staff, program participants, and advisory board members. For example, using maps to understand what languages people speak at home strengthens the connections between Extension programs and community participants and can inform staff recruitment and advisory board composition. However, maps of aggregated areas like counties can mask socioeconomic …

Strengthening Urban Food Systems Through Extension Programming And Community Engagement: A Case Study Of New Brunswick, New Jersey, Cara L. Cuite, Lauren B. Errickson

Journal of Human Sciences and Extension

Cooperative Extension (Extension) can, and in many cases already does, engage in well-rooted partnerships with urban audiences. Yet, it is important to recognize that there are many layers to the diversity that exists within urban audiences– there is no single “urban community.” This article presents a case study of food security programming in New Brunswick, New Jersey, including collaborations between Rutgers Cooperative Extension and multiple community organizations to illustrate important considerations for engaging in urban Extension initiatives. Specifically, challenges exist in identifying urban audiences, including those who are hidden, especially as the demographics of city residents can vary greatly within …

Cooperative Extension In Urban America: Place-Based Approaches For Improving Health, Dawn Burton, Latoya O'Neal, Erin Yelland, Suzanne Stluka, Rodger Rennekamp

Journal of Human Sciences and Extension

While the bulk of Cooperative Extension’s (Extension) historical work has been with rural populations, its future work should also address the needs of those living in urban areas. The opportunity to live a long and healthy life is paramount among those needs. Cooperative Extension’ National Framework for Health Equity and Well-Being (Framework) provides a roadmap by which Cooperative Extension can help ensure that all people have that opportunity. The central premise of the Framework is that future work of Extension must include but extend beyond the promotion of healthy behaviors to place-based approaches for improving conditions in which people live, …

Reconceptualizing Youth Sparks: A Sociocultural Approach To Co-Designing Programs For Somali Youth, Joanna A. Tzenis

Journal of Human Sciences and Extension

The 4-H Youth Development Program has a long history of fostering positive youth outcomes. Recently, attention and resources have been invested in the development of a program model (i.e., the 4-H Thriving Model) that theorizes the program elements that lead to positive outcomes (Arnold, 2018). Less attention, however, has been given to the program design process by which and with whom 4-H programs are designed and implemented. This matters because a lack of a community-engaged design process may lead to outcomes disconnected from community self-interests or to the exclusion of youth who might view the program as irrelevant to their …

Book Review – The Art Of Being Indispensable At Work: Win Influence, Beat Overcommitment, And Get The Right Things Done, Eric Killian

Journal of Human Sciences and Extension

With new technology, constant change, uncertainty, and virtual teams, getting things done at work is tougher and more complex than ever. Finding your place amid the collaboration revolution working with everyone across silos and various platforms can feel like we are in a no-win cycle in urban Extension environments. However, there is always the indispensable go-to person, or in our case organization—urban Extension, that thrives on many working relationships, maintains a positive attitude of service, demonstrates creativity and tenacity, and strives to get the right things done. This review will share information on how urban Extension professionals can continue to …

Fostering A Sense Of Belonging In Urban Extension For Internal And External Stakeholders, Ramona Madhosingh-Hector, Linda M. Seals

Journal of Human Sciences and Extension

In urban areas, the communities are as diverse as the issues, and different educational and engagement strategies must be deployed to support urban Extension clientele. Urban communities must connect with and feel a sense of “belonging” with Extension–this connection can strengthen Extension’s presence and value proposition to urban constituents. This study found that although Extension was engaged in efforts to support belonging, its efforts were hampered by a lack of collaboration and commitment to supporting a system-wide effort to change the culture. Intentionality, emotional intelligence, and dialogue were presented as strategies to foster a sense of belonging for Extension personnel …

Futuring Perspectives And Practices For Urban Extension, Julie Fox, Solomon Garner

Journal of Human Sciences and Extension

Extension is well-positioned to engage strategically with urban communities now and in the future. A century of collaboration and proven impact provides Extension with a valuable foundation focused on relevant service accessible to all people; applied science and co-discovery; respectful and inclusive engagement; and commitment to community well-being. An exploration of the literature on the future, from the Extension perspective and from external viewpoints, informs a contemporary approach to focus on the future of urban Extension. An interplay of Extension professionals with diverse community stakeholders and dynamic technologies bridges past perspective and current context with practical methods to address the …