Life Sciences Commons^™

Farming For The Future; Managing Lowland Grassland For Biodiversity, Sally Griffin

ORBioM (Open Research BioSciences Meeting)

Grassland is Ireland’s most expansive terrestrial habitat type covering >58% of the land cover but its biodiversity is under serious threat of agricultural intensification or land abandonment. While the decline in Irish grassland biodiversity has been recognised, there is a paucity of research into the restoration of lowland mesotrophic grassland. Most of Ireland’s grasslands are privately farmed mainly for pasture, therefore, appropriate agricultural management techniques for biodiversity restoration are vital. The purpose of this research was to first classify the lowland grassland vegetation in Killarney National Park, Co. Kerry in the west of Ireland (Approx. 244ha). Then to investigate selected …

Genetic Variations In The Myostatin Gene Among Irish Cattle Breeds, Cliona Ann Ryan

ORBioM (Open Research BioSciences Meeting)

No abstract provided.

Re-Assessing The Importance Of Linear Type Traits In Predicting Genetic Merit For Survival In An Aging Holstein-Friesian Dairy Cow Population., Maeve Williams

ORBioM (Open Research BioSciences Meeting)

Background

Linear type traits were originally scored as a means of predicting functionality manifesting itself as greater cow longevity. Existing studies relating linear type traits to dairy cow longevity were generally restricted to young cows. Improved reproductive performance, however, is contributing to an aging cow population. Therefore, the objective of this study was to evaluate if the genetic correlations between linear type traits and survival in each parity changes as cows age.

Methods

After edits, 152,894 lactation survival records (1st to 9th parity) were available from 52,447 Holstein-Friesian dairy cows, along with linear type trait records from 52,121 Holstein-Friesian dairy …

Validation Of The Irish National Sheep Genetic Evaluations, Niamh Barry

ORBioM (Open Research BioSciences Meeting)

Background: In Ireland, national sheep genetic evaluations have been implemented since 2009, however, to date no large scale study has been undertaken to investigate the accuracy of genetic evaluations in a large multi-breed population. The aim of this study was to validate the estimated breeding values (EBV) on a range of traits, using national data comprising 203,469 records across a range of phenotypic traits from 9,377 flocks.

Methods: The association between direct and maternal EBV on a range of lamb growth traits was quantified using a fixed effects model. For the lambing traits the association between direct and maternal EBV …

Short Chain Fatty Acid Combination Treatment Protects Against 6-Ohda Induced Decrease In Neurite Growth In An In Vitro Model Of Parkinson’S Disease., Alex Morris

ORBioM (Open Research BioSciences Meeting)

Title: Short chain fatty acid combination treatment protects against 6-OHDA induced decrease in neurite growth in an in vitro model of Parkinson’s disease.

Authors: Alex Morris¹, Louise M. Collins^1,2,3, Gerard W. O’Keeffe², Caitriona M. Guinane¹

¹ Department of Biological Sciences, Munster Technological University (MTU), Cork, Ireland.

² Department of Anatomy & Neuroscience, University College Cork, Cork, Ireland.

³ Department of Physiology, University College Cork, Cork, Ireland.

Background: Parkinson’s disease (PD) is a neurodegenerative disorder characterized by dopaminergic neuron degeneration. This leads to motor dysfunction which is accompanied by gastrointestinal comorbidities such as constipation …

Growth Of Foodborne Pathogens Listeria And Salmonella And Spore-Forming Paenibacillus In Commercial Plant-Based Milk Alternatives, Klaudia Bartula

ORBioM (Open Research BioSciences Meeting)

An increase in vegan diet preference, lactose intolerance, calorie concern and environmental awareness has led to a rise in the popularity of plant-based alternatives to bovine milk. Non-dairy products are a fast-growing food industry sector; however, there are still gaps in understanding how the known bacterial food contaminants behave in plant-based beverages. The present study is the first to compare the growth of food-pathogens Listeria monocytogenes and Salmonella enterica and an industrial milk product isolate, spore-forming Paenibacillus, in commercially available ultrahigh temperature processed (UHT) bovine milk and plant-based milk alternatives (coconut, almond, cashew). Beverage samples were inoculated with a strain …

An Analysis Of Available European Food Composition Databases To Enable The Development Of A Technology-Based Dietary Assessment Tool, Hannah Al-Sehaim

ORBioM (Open Research BioSciences Meeting)

DIETARY DEAL is an EU-project led by MTU, tasked to develop an open-source dietary assessment tool (DAT) to harmonise collection and analysis of dietary data across Europe⁽¹⁾. A key component of all DATs is integrated food composition databases (FCD) containing energy and nutrient information (per 100g food) for translation of food intake data into nutrient estimates.

This preliminary research aimed to identify and quantify available FCD in European countries for inclusion in the new DAT.

Search terms such as [European Food Composition Databases], [Available Food Composition Databases in Europe] and [Recent European Food Composition Databases Published] were used …

Tunable Diode Laser Absorption Spectroscopy As An Alternative Method For Non-Invasive Sterility Detection In Ready To Feed Infant Milk Products, Peter Myintzaw

ORBioM (Open Research BioSciences Meeting)

Background: The potential application of rapid, non-destructive, and user-friendly TDLAS technology to detect contamination in commercially sterile dairy beverages where visual inspection is not possible was investigated for the first time. The TDLAS equipment uses laser light to monitor carbon dioxide changes due to microbial growth in the container headspace which has the advantage of being rapid and non-destructive. This study aimed to provide detailed scientific evidence for the application of TDLAS technology as a method to determine product sterility in real food products.

Methods: TDLAS growth detection of Bacillus fengqiuensis, Candida albicans, Lactococcus lactis, Microbacterium …

Mining Of Producer Recorded Data; Using Beef Calf And Cow Live-Weight Data As A Case Study, Shauna Walsh

ORBioM (Open Research BioSciences Meeting)

Animal live-weight contributes to profitability in beef herds and is a key determinant of overall efficiency of the beef sector. The objective was to develop a novel editing criteria for anomaly detection of beef cow and calf live-weight data. Live-weight data from five sources (i.e., professionally-recorded, owned-scales, borrowed-scales, scales hired from a depot, other) were available from the Irish Cattle Breeding Federation.

A number of alternative methods were used for anomaly detection including: generation of within-herd regression estimates, partial correlations between cow and calf live-weight records and mahalanobis distance. Across each method a value was calculated for each herd based …

Investigation Of The Antimicrobial Activity Of Coagulase Negative Staphylococci Of Human Origin, Ellen Twomey

ORBioM (Open Research BioSciences Meeting)

Coagulase Negative Staphylococci (CoNS) are significant members of the human microbiome and have been identified as a source of bacteriocins, small bioactive substances recognised for their antimicrobial properties. In this study we screened a bank of 37 human derived CoNS for the production of these peptides with emphasis on identifying those with the ability to inhibit the growth of Methicillin Resistant Staphylococcus aureus (MRSA). Deferred antagonism assays confirmed potential antimicrobial activity in all 37 isolates against sensitive indicator M. luteus, with nine inhibiting the growth of at least one MRSA strain they were overlaid against. Tests performed with the …

Dietary Fibre Intake In Teenagers (13-18 Years) Living In Ireland, Cliodhna Mcgowan

ORBioM (Open Research BioSciences Meeting)

Dietary fibre (DF) plays a major role in normal bowel function and low intakes have been reported globally for all population groups including teenagers. The aim of this study was to use new data from the National Teens’ Food Survey II (2019-20) to estimate intake and sources of DF for teenagers in Ireland.

Dietary intake data were collected using a 4d weighed food-diary and DF intake was estimated using UK and Irish food composition data. The mean daily intake (MDI) of DF was calculated via the NCI-method and compared with the adequate intake (AI) of 19g/d (13-14-year-olds) , 21g/d 1(5-17-year-olds) …

Effect Of Sub-Inhibitory Concentrations Of Nitrofurantoin, Ciprofloxacin And Trimethoprim On In-Vitro Biofilm Formation In Uropathogenic Escherichia Coli, Shane Whelan

ORBioM (Open Research BioSciences Meeting)

The purpose of the current study was to determine the effect of sublethal concentrations of nitrofurantoin, ciprofloxacin and trimethoprim on biofilm formation in 57 uropathogenic Escherichia coli strains (UPEC).

The MIC of nitrofurantoin, ciprofloxacin and trimethoprim was determined for 57 UPEC isolates. Biofilm formation for each isolate with and without sub-lethal concentrations of each antibiotic was then quantified, and the statistical significance of changes in biofilm formation was ascertained by way of a Dunnett's test.

The effects of sub-MIC antibiotics on biofilm formation of UPEC were variable. A total of 22.8% of strains were induced to form biofilm by nitrofurantoin, …

An Investigation Of The Novel Use Of Bacteriophages To Diagnose And Treat Johne's Disease In Cattle, Max Kevane-Campbell

ORBioM (Open Research BioSciences Meeting)

Johne’s disease is a scourge to dairy farmers all over the world. It is an infectious disease that causes chronic inflammation and lesions along the inside of the small intestine of, primarily, ruminant animals (i.e., cattle and sheep). It is an incurable disease and urgently requires new and radical intervention strategies. Apart from careful on-site farm management practices, little can be offered to farmers to reduce the risk of infection, and nothing short of livestock culling is effective once an animal becomes infected. Currently, there are no vaccines licensed in Ireland or antibiotic treatment strategies available for Johne’s disease. This …

Investigation Of The Urobiome For The Production Of Novel Antimicrobials Against Uropathogenic E. Coli (Upec), Jennifer Jones

ORBioM (Open Research BioSciences Meeting)

BACKGROUND: Urinary tract infections (UTIs) are one of the most common bacterial infections globally with antibiotic treatment becoming increasingly less effective. The urobiome remains a relatively understudied niche as a source of potentially novel antimicrobials (e.g., bacteriocins). Improvements to bacterial culturing and sequencing techniques have highlighted the potentially rich source of alternative treatments and control strategies to target uropathogenic E. coli (UPEC).

MATERIALS AND METHODS: (a) BAGEL 4 was used for bioinformatic screening of the genomes of urobiome isolates to identify bacteriocin gene clusters (BGC).

(b) Expanded quantitative urine culture (EQUC) was used to culture mid-stream urine samples to isolate …

'Investigating The Effect Of Cell-Free Supernatant Of Probiotic Strains On The Viability Of Pancreatic Cancer Cell Lines', Claire M. Doocey

ORBioM (Open Research BioSciences Meeting)

Background

Pancreatic cancer (PC) is a prevalent cancer accounting for over 450,000 deaths globally in 2020, and is associated with limited treatment options and high mortality rates. Mounting evidence implicates the human microbiome in tumorigenesis and cancer progression with key players including Helicobacter pylori in gastric cancer and Fusobacterium nucleatum in colorectal cancer and PC. Current research, however, also suggests a potential role for certain microbial residents in oncological treatment and biotherapeutic development. Recent studies have shown a link between cell-free supernatants (CFS) from probiotic strains and cancer cell death, suggesting their potential in targeting the tumour microenvironment. Probiotic genera …

Genetic Trends For Carcass Traits Within Breeds In A Multi-Breed Evaluation, Shauna Mulhall

ORBioM (Open Research BioSciences Meeting)

Most genetic evaluations and breeding objectives worldwide for beef traits tend to be on a within breed basis. Ireland is relatively unique in publishing national multi-breed genetic and genomic evaluation for carcass traits. The focus of this research is to determine genetic trends in carcass traits within each of the common sire breeds used in both dairy and beef populations. Estimated breeding values for carcass weight, carcass conformation, and carcass fat were available on 8,375,561 animals from the national carcass genetic evaluation. The mean of each animal’s sire EBV was estimated for each animal’s birth year for carcass weight, carcass …

Application Of Fructans-Degrading Lachancea Fermentati Fst5.1 As An Alternative To Baker’S Yeast In The Production Of A Low-Fodmap Whole Wheat Bread., Małgorzata Borowska

ORBioM (Open Research BioSciences Meeting)

FODMAPs are a group of poorly absorbed dietary carbohydrates comprised of easily fermentable oligo-, di-, monosaccharides and polyols. When consumed, they can exert gastrointestinal symptoms of pain, flatulence, or an irregular bowel movement in individuals with irritable bowel syndrome. Whole wheat bread is an excellent source of proteins, vitamins, polyphenols, and other nutrients, but its consumption is restricted in a low-FODMAP diet due to high fructans content. Although alternatives exist, these are primarily gluten-free, often inferior in terms of quality, nutritional profile, and consumer acceptance. Bioprocessing using microbial fermentation has a proven capacity to reduce FODMAPs in cereal products, but …

Evaluating 3d Printing As A Technology For Producing Dairy Snacks Based On Temperature-Controlled Rennet Induced Gelation Of Protein-Fortified Milk., Ricardo Uribe Alvarez

ORBioM (Open Research BioSciences Meeting)

3D food printing (3DFP) has the potential to develop products with complex structures, personalised nutrition, and customised textures. However, only foods with suitable physicochemical or rheological properties are suitable for 3DFP to ensure a self-supported structure is printed. Milk proteins can form structured objects (i.e., cheese, yoghurt), and so are potential ingredients for 3DFP. This study aims to adapt the rennet gelation from the curdling process of cheese-making to produce 3D printed dairy snacks using milk protein isolate-fortified milk. It is well known that curdling of milk by rennet is temperature dependant; therefore, the rennet gelation properties (G' and G'') …

Corrigendum To “The Impact Of The Rare Disease And Orphan Drug Act In Taiwan” [J Food Drug Anal 29 (2021) 717-725]

Journal of Food and Drug Analysis

No abstract provided.

The Study Of Distribution Of Ingested Terbinafine On Skin With Ambient Ionization Tandem Mass Spectrometry, Yi-Tzu Cho, Hung Su, Ching-Ying Wu, Jingyueh Jeng, Chi-Wei Lee, Deng‐Chyang Wu, Tiao-Lai Huang, Jentaie Shiea

Journal of Food and Drug Analysis

We aim to develop an efficient and non-invasive strategy for monitoring of drugs and their metabolites via human skin. Probe sampling was combined with thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) to characterize trace terbinafine, which was secreted on patient’s skin after ingesting terbinafine tablets. The terbinafine ion signals were directly detected in the samples collected from different skin regions and the signals were monitored for 8 weeks. The detection and location of terbinafine via the skin suggest that the methods are useful in rapidly and noninvasively collecting the molecular information of the ingested drug on skin for pharmacokinetic studies.

Chemiluminescence Of Lucigenin-Tetracycline And Itsapplication For Sensitive Determination Of Procyanidin, Shuai Quan, Kaixiang Ji, Fangshuo Liu, Tesfaye Hailemariam Barkae, Mohamed Ibrahim Halawa, Saima Hanif, Baohua Lou, Jianping Li, Guobao Xu

Journal of Food and Drug Analysis

Tetracycline was reported to trigger intense lucigenin chemiluminescence (CL). Based on its significant quenching to lucigenin-tetracycline CL, procyanidin was detected. The mechanism of the lucigenin-tetracycline CL and its quenching by procyanidin were proposed. Under the optimum conditions, the calibration curve is linear in the range of 10-1000 nM for procyanidin with a detection limit of 4.4 nM. The RSD for nine replicate determinations of procyanidin was less than 1.7%. Moreover, this method was satisfactorily applied to the determination of procyanidin in human urine samples and health food capsule of grape seed.

Quantitation Of Mycophenolic Acid And Metabolites By Uplc-Ms/Ms In Renal Transplant Patients, Yun-Fen Huang, Ya-Ching Huang, Hsiao-Chen Ning, Chia-Ni Lin

Journal of Food and Drug Analysis

Mycophenolate mofetil (MMF) and enteric-coated mycophenolate sodium (EC-MPS), specific inhibitors of lymphocyte proliferation, are commonly used as adjuvant therapy with calcineurin inhibitor agents after kidney transplantation. After administration, MMF and EC-MPS are hydrolyzed to mycophenolic acid (MPA), the active form of the drug, which must be monitored due to its narrow therapeutic window, drug–drug interactions, and large intra-and inter-individual pharmacokinetic variability despite a fixed dose. Monitoring plasma MPA level is recommended to maintain the drug within the therapeutic window, optimize its efficacy, and minimize side effects. This study aims to develop a method for quantifying MPA and its major metabolites …

Camphorataimide B Suppresses The Metastasis Of Human Colorectal Cancer Cell By Inhibiting Smad/Fak/Akt Axis And Promoting Degradation Of Snail/Bmp4 Complex, Chi-Chou Huang, Chia-Hung Hung, Yi-Ju Lee, Tsui-Hwa Tseng, Yean-Jang Lee, Shao-Hsuan Kao, Chau-Jong Wang

Journal of Food and Drug Analysis

Camphorataimide B (CamB) has anticancer activities against several tumors. Here, we aimed to investigate the mechanism(s) by which CamB inhibits metastasis of colorectal cancer (CRC) cells. Low-dose CamB did not affect the cell viability and cell cycle progression of CRC cells, but significantly inhibited the metastatic potentials of CRC cells. Mechanically, CamB reduced the protein and mRNA expression of BMP4, and inhibited Smad and FAK/Src/Akt signaling. CamB also decreased Snail levels by promoting its degradation via proteasome and thereafter reduced Snail-mediated BMP4 transcription. Moreover, CamB considerably inhibited the in vivo metastasis of DLD-1 cells in xenograft mice.

Orally Delivered Perilla (Perilla Frutescens) Leaf Extract Effectively Inhibits Sars-Cov-2 Infection In A Syrian Hamster Model, Yuan-Fan Chin, Wen-Fan Tang, Yu-Hsiu Chang, Tein-Yao Chang, Wen-Chin Lin, Chia-Yi Lin, Chuen-Mi Yang, Hsueh-Ling Wu, Ping-Cheng Li, Jun-Ren Sun, Shu-Chen Hsu, Chia-Ying Lee, Hsuan-Ying Lu, Jia-Yu Chang, Jia-Rong Jheng, Cheng Cheung Chen, Jyh-Hwa Kau, Chih-Heng Huang, Cheng-Hsun Chiu, Yi-Jen Hung, Hui-Ping Tsai, Jim-Tong Horng

Journal of Food and Drug Analysis

On analyzing the results of cell-based assays, we have previously shown that perilla (Perilla frutescens) leaf extract (PLE), a food supplement and orally deliverable traditional Chinese medicine approved by the Taiwan Food and Drug Administration, effectively inhibits SARS-CoV-2 by directly targeting virions. PLE was also found to modulate virus-induced cytokine expression levels. In this study, we explored the anti-SARS-CoV-2 activity of PLE in a hamster model by examining viral loads and virus-induced immunopathology in lung tissues. Experimental animals were intranasally challenged with different SARS-CoV-2 doses. Jugular blood samples and lung tissue specimens were obtained in the acute disease stage (3−4 …

Assessing The Fate Of Fatty Acid Esters Of Hydroxy Fatty Acids, Diglycerides And Monoacetyldiacylglycerides In Grilled Ruminant Meats Marinated With Unfiltered Beer-Based Marinades, Charles F. Manful, Thu H. Pham, Evan Wheeler, Muhammad Nadeem, Oludoyin A. Adigun, Nicole Walsh, Raymond H. Thomas

Journal of Food and Drug Analysis

Ruminant meats contain functional lipids including fatty acid esters of hydroxy fatty acids (FAHFA), diglycerides (DG), monoacetyldiglycerides (MAcDG) and medium chain triglycerides (McTG) whose consumption in the normal diet can confer benefits for consumer health. However, very little is known concerning how meat processing techniques such as marination and grilling affect the quantity and quality of these functional lipids in ruminant meats. We used ultra high-performance liquid chromatography coupled to high resolution accurate mass tandem mass spectrometry (UHPLC-HRAM-MS/MS) to show how grilling following marination with either India or Wheat ale unfiltered beer-based marinades affected the quantity and quality of these …

Lc-Qtof-Ms Evaluation Of Rabbit-Specific Peptide Markers For Meat Quantitation, Anna Stachniuk, Anna Kozub, Renata Czeczko, Magdalena Montowska, Emilia Fornal

Journal of Food and Drug Analysis

Ten rabbit-specific tryptic peptide markers and one marker peptide specific to both rabbit and hare were evaluated for mass signal linearity in binary meat mixtures using liquid chromatography-quadrupole time-of-flight-mass spectrometry. Seven meat mixtures containing chicken and varying percentages of rabbit (1%, 5%, 10%, 30%, 60%, 90%, and 100%) were analyzed. Additionally, the signal linearity of twelve peptide markers for chicken meat was examined. The best candidate peptides for the quantification of meat content were selected. Five of eleven peptides for rabbit meat and five of twelve peptides for chicken meat showed good linearity (R2 > 0.97). The limits of detection and …

Exosomal Proteomics In Kidney Disease: From Technical Approaches To Clinical Applications, Ageng Brahmadhi, Yung-Kun Chuang, San-Yuan Wang, Chih-Chin Kao, I-Lin Tsai

Journal of Food and Drug Analysis

Exosomes are small extracellular vesicles (sEVs) secreted from cells and have a general diameter ranging from 30~150 nm. It was reported that exosomes have essential roles in intercellular communication and can be targeted as biomarkers of disease or as therapeutic agents. Among the different techniques used for exosome investigation, the mass spectrometry-based proteomics approach has accelerated the unraveling of the molecular composition of exosomes and has contributed to improved knowledge of molecular processes in various diseases. In this review, we focused on proteomics-based studies of exosomes and clinical applications in kidney diseases. A general introduction of exosomes, isolation and characterization …

Differential Scanning Calorimetry For Authentication Of Edible Fats And Oils - What Can We Learn From The Past To Face The Current Challenges?, Mahbuba Islam, Liliana Bełkowska, Piotr Konieczny, Emilia Fornal, Jolanta Tomaszewska-Gras

Journal of Food and Drug Analysis

Fats and oils authentication has become an important issue recently, due to the growing interest in consumption of cold-pressed oils. Therefore, it is becoming more and more difficult to maintain official control over the growing assortment of new cold-pressed oils. Authenticity of plant oils is also an important issue for religious or cultural reasons.This review article focuses on the application of differential scanning calorimetry (DSC) in the field of assessing authenticity of various fats and oils (e.g. olive oil, palm oil, confectionery fats, butter). Extra virgin olive oil (EVOO) is the most comprehensively tested oil by means of the DSC …

Trends And Applications Of Food Protein-Origin Hydrolysates And Bioactive Peptides, Yi-Hsieng Samuel Wu, Yi-Chen Chen

Journal of Food and Drug Analysis

It was reported that protein hydrolysates or derived peptides have more functionalities than their parent protein. Most functional protein hydrolysates or peptides are identified from various food products, including plant, fish, and land-animal protein sources. Within a few decades, the application of food protein-origin functional hydrolysates or peptides could be divided into two main categories according to their applied intentions: 1) preservatives and bioactive packing materials; 2) nutraceutical ingredients. According to the literature, the applications of food protein-origin functional hydrolysates or peptides on food preservative and nutraceutical ingredients have attracted much attention. However, the approach method should be changed. Multi-activities, …

Neutrophil: Lymphocyte Ratio And Platelet: Lymphocyte Ratio Related To Disease Activity Among Rheumatoid Arthritis In Erbil Governorate/Iraq, Zekra Ali Aziz, Dashty A. Al-Bustany

Polytechnic Journal

Rheumatoid Arthritis (RA) is a systemic autoimmune inflammatory disorder that causes joints destruction the aim here is to investigate the neutrophil - to - lymphocyte ratio (NLR) and platelet - to- lymphocyte ratio (PLR) in RA patients and relation to Disease Activity Score of 28 joints (DAS28-ESR). The study population included (260) participants which consist of (210) RA patients and (50) healthy persons as a control. RA patients were two groups (107) received conventional diseases modified anti-rheumatic drugs (cDMARD) and (103) received biological agents (bDMARD) plus cDMARD each group subdivided into disease active patients (DAS28-ESR ≥ 2.6) and patients in …