Life Sciences Commons^™

Assessing Environmental Sensitivity In San Diego County, California, For Bird Species Of Special Concern, Eda Okan Kilic

USF Tampa Graduate Theses and Dissertations

It is widely recognized that desertification constitutes one of the biggest environmental problems on Earth. Desertification negatively impacts the future of humans and other living things all over the globe. Therefore, the assessment of desertification is essential to both monitor and combat desertification. A number of models are routinely applied to assess desertification. The MEDALUS model is one of the most popular approaches, identifying desertification risk based on an environmentally sensitive area index (ESAI) that integrates climate, vegetation, soils, groundwater, and socio-economic factors to obtain an overall rating. The goal of this study was to measure the potential impacts of …

The Three Two-Pore Channel Subtypes From Rabbit Exhibit Distinct Sensitivity To Phosphoinositides, Voltage, And Extracytosolic Ph, Xinghua Feng, Jian Xiong, Weijie Cai, Jin-Bin Tian, Michael X Zhu

Faculty, Staff and Student Publications

Two pore channels (TPCs) are implicated in vesicle trafficking, virus infection, and autophagy regulation. As Na+- or Ca2+-permeable channels, TPCs have been reported to be activated by NAADP, PI(3,5)P2, and/or high voltage. However, a comparative study on the function and regulation of the three mammalian TPC subtypes is currently lacking. Here, we used the electrophysiological recording of enlarged endolysosome vacuoles, inside-out and outside-out membrane patches to examine the three TPCs of rabbit (Oryctolagus cuniculus, or Oc) heterologously expressed in HEK293 cells. While PI(3,5)P2 evoked Na+ currents with a potency order of OcTPC1 > OcTPC3 > OcTPC2, only OcTPC2 displayed a strict dependence …

Hematologic Complications Of Immune Checkpoint Inhibitors, Michael H Kroll, Cristhiam Rojas-Hernandez, Cassian Yee

Faculty, Staff and Student Publications

Immune checkpoint inhibitors are a class of antineoplastic therapies that unleash immune cells to kill malignant cells. There are currently 7 medications that have been approved by the US Food and Drug Administration for the treatment of 14 solid tumors and 2 hematologic malignancies. These medications commonly cause immune-related adverse effects as a result of overactive T lymphocytes, autoantibody production, and/or cytokine dysregulation. Hematologic toxicities are rare and of uncertain mechanism, and therefore management is often based on experiences with familiar conditions involving these perturbed immune responses, such as autoimmune hemolytic anemia, immune thrombocytopenia, and idiopathic aplastic anemia. Management is …

High Tunnel Construction Cost Calculation Workbook, Shannon Rauter, Melanie Stock, Brent Black, Dan Drost

All Current Publications

The cost of building a high tunnel fluctuates over time with supply prices. This Excel workbook accompanies the USU Extension fact sheet, "Constructing a Low-cost High Tunnel" by Black, Drost, Rowley, and Heflebower (2011) and allows users to input local supply costs. The workbook then calculates the cost to build a USU low-cost high tunnel with several design options according to high tunnel length and door structure. It also provides an example calculation based on April 2022 pricing for reference.

Urinary Aflatoxin M1 Concentration And Its Determinants In School-Age Children In Southern Ethiopia, Tafere Gebreegziabher Belay, Melanie Dean, Elilta Elias, Workneh Tsegaye, Barbara J. Stoecker

All Faculty Scholarship for the College of Education and Professional Studies

Aflatoxins are mycotoxins that can contaminate grains, legumes, and oil seeds. These toxic compounds are an especially serious problem in tropical and sub-tropical climates. The objective of this study was to raise awareness of aflatoxin exposure among primary school children in Shebedino woreda, southern Ethiopia, by measuring urinary aflatoxin M1 (AFM1). The study employed a cross-sectional design and systematic random sampling of children from eight schools in the district. The mean ± SD age of the children was 9.0 ± 1.8 years. Most (84.6%) households were food insecure with 17.9% severely food insecure. Urinary AFM1 was detected in more than …

‘Bunkering Down’: How One Community Is Tightening Social-Ecological Network Structures In The Face Of Global Change, Michele L. Barnes, Lorien Jasny, Andrew Bauman, Jon Ben, Ramiro Berardo, Örjan Bodin, Josh Eli Cinner, David A. Feary, Angela M. Guerrero, Fraser A. Januchowski-Hartley, John T. Kuange, Jacqueline D. Lau, Peng Wang, Jessica Zamborain-Mason

Marine & Environmental Sciences Faculty Articles

1. Complex networks of relationships among and between people and nature (social-ecological networks) play an important role in sustainability; yet, we have limited empirical understanding of their temporal dynamics.
2. We empirically examine the evolution of a social-ecological network in a common-pool resource system faced with escalating social and environmental change over the past two decades.
3. We first draw on quantitative and qualitative data collected between 2002 and 2018 in a Papua New Guinean reef fishing community to provide contextual evidence regarding the extent of social and environmental change being experienced. We then develop a temporal multilevel exponential random graph model using …

Why Are There So Many Mosquitoes?, Alan L. Gillen, Savannah Childs, Megan Goin, Karly Mckinney

Faculty Publications and Presentations

Mosquitoes have been challenging man and animals alike since the fall.Mosquitoes number in the trillions worldwide. Not only is the mosquito a nuisance with the constant buzz around your ear, they also are the primary vector of many dangerous diseases including the West Nile Virus (WNV). WNV has become a more prominent mosquito-transmitted disease in the United States and more specifically in Colorado, Texas, and other states in the Midwest. While commonly found in the genus Culex, WNV and other diseases can be found in differentgenera of mosquito, including Aedes and Anopheles. While these two are not themost common regarding …

The 30x30 Program: A Federal Land Grab?, J. David Aiken

Cornhusker Economics

In January 2021, the Biden Administration issued a climate policy executive order.[2] [or EO].[3] Section 216 of the Biden climate EO, Conserving & Protecting our Nation’s Lands & Waters, directed the U.S. Department of the Interior, the U.S. Department of Agriculture, and the U.S. Department of Commerce (which includes NOAA, the U.S. National Oceanic & Atmospheric Administration) to prepare a report “recommending steps that the United States should take, working with state, local, tribal and territorial governments, agricultural and forest landowners, fishermen and other key stakeholders to achieve the goal of conserving at least 30% of our lands and waters …

Interlaboratory Comparison Of Metabolomics Analyses Of Human And Rodent Blood Using Biocrates Mxp Quant 500 Kit, Nadia Ashrafi, Ali Yilmaz, Stewart F. Graham, Thomas O'Connell, Sumeyya Akyol

Conference Presentation Abstracts

No abstract provided.

Continuous Effort Required To Maintain Populations Of Outplanted Acropora Cervicornis In The Florida Reef Tract, Usa, Tiffany S. Boisvert

USF Tampa Graduate Theses and Dissertations

The degradation of coral reefs due to natural and anthropogenic stressors has resulted in the expansion of coral restoration projects worldwide. In the Caribbean region, most restoration efforts focus on outplanting Acropora cervicornis, once a dominant branching coral, now found predominantly in spatially isolated populations. Thousands of A. cervicornis colonies are propagated within nurseries and outplanted onto degraded reefs every year. However, monitoring the long-term growth and survival of outplanted corals has been limited by financial, physical, and temporal constraints. In the current study, we assessed the long-term success of A. cervicornis restoration by determining the relationship between current populations …

The Role Of The Gut-Brain Axis In Alzheimer's Disease: A Narrative Review, Leila Karout

University Honors Theses

The importance of the gut microbiome in health has become increasingly recognized as more research points to the strong association between an imbalanced gut microbial composition and a diseased state. Alzheimer's Disease burdens millions, and the rate of growth is expected to increase. However, current treatment methods are less than ideal and do not offer a substantial benefit to patients' quality of life. Additionally, there are gaps in the current understanding of Alzheimer's Disease pathogenesis. Research on the relationship between gut microbiota and neurodegeneration provides a different perspective on the pathogenesis and treatment methods for dementia, such as Alzheimer's Disease. …

A Participatory Assessment Of Nitrified Urine Fertilizer Use In Swayimane, South Africa: Crop Production Potential, Farmer Attitudes And Smallholder Challenges, Benjamin C. Wilde, Eva Lieberherr, Engil Isadora Pujol Pereira, Alfred Odindo, Johan Siz

School of Earth, Environmental, & Marine Sciences Faculty Publications

Long-term nutrient mining of soil hampers agricultural production across Africa. However, emerging sanitation technologies afford a hygienically safe and ecologically sustainable solution to this development challenge by providing fertilizers derived from human excreta that could facilitate a socio-technical transition toward a more sustainable food system. To evaluate one such technology, nitrified urine fertilizer (NUF), we conducted participatory action research to assess the potential, from both a biophysical and social perspective, of NUF to serve as a soil fertilizer to support smallholder agricultural production in Swayimane, South Africa. To achieve this objective, we formed a stakeholder group comprised of a cooperative …

Etiology Of Sterile Intra-Amniotic Inflammation: An Exploratory Study, Zoe M. Taylor

USF Tampa Graduate Theses and Dissertations

Preterm birth is the event of spontaneous birth prior to 37 weeks of gestation. In the United States, 1 in 10 babies are born prematurely. Low gestational age has been linked with increased morbidity and mortality. Preterm birth is multifactorial, where a myriad of contributors have been identified to include inflammation, chorioamnionitis, and inflammatory conditions to include hypertension and diabetes. To further explore the role inflammation plays in low gestational age, a literature review was done using PubMed. Two classes of inflammation quickly emerged: Pathogen born intra-amniotic inflammation and Sterile intra-amniotic inflammation. The latter form of inflammation has no identifiable …

Sustainable Intensification Of Wheat Production Under Smallholder Farming Systems In Burera, Musanze And Nyamagabe Districts Of Rwanda, Athanase Rusanganwa Cyamweshi, Nsharwasi Léon Nabahungu, Jackson Cirhuza Mirali, Job Kihara, George Oduor, Harrison Rware, Gudeta Weldesemayat Sileshi

All Peer-Reviewed Publications

The productivity of wheat is low on smallholder farms in Rwanda. Although mineral fertiliser use is being promoted as a sustainable intensification (SI) pathway, little is known about the nutrient use efficiency and profitability of various fertiliser inputs in Burera, Musanze and Nyamagabe districts of Rwanda. The objective of this study was to assess the use of combinations of nitrogen (N), phosphorus (P), potassium (K), magnesium (Mg), zinc (Zn) and boron (B) in wheat production in terms of nutrients management specifically, crop yield, production risk, input use efficiency and economic returns on smallholder farms. The study was conducted in three …

Links Between Soilborne Pathogens, Plant Parasitic Nematodes, Farm Management And Biophysical Constraints In A Southern Australian Rainfed Cropping System, Martin Harries, Ken C. Flower, Michael Renton, Sarah J. Collins, Daniel Huberli

Grain and Other Field Crops Research Articles

Context: Rotations in rainfed farming systems of southwest Australia have shifted towards intensified cropping and it is necessary to reassess soilborne pathogens and plant parasitic nematodes within this context. Aims: We tested the hypothesis that these recent changes in rotations and agronomy have altered the efficacy with which rotations reduce the incidence of common root pathogens and plant parasitic nematodes. Methods: We tracked changes in common pathogen DNA in soil and the incidence and severity of crop root damage in 184 paddocks, over 6 years from 2010 to 2015, and related this to farmer practices. Key results: Overall, severe root …

Effect Of Neoadjuvant Chemotherapy On Intraoperative Core Temperature In Patients With Breast Cancer: A Retrospective Cohort Study, Daniel B Zamler, Takashi Shingu, Laura M Kahn, Kristin Huntoon, Cynthia Kassab, Katarzyna Tomczak, Yating Li, Ivy Lai, Rocio Zorilla-Veloz, Cassian Yee, Kunal Rai, Betty Ys Kim, Stephanie S Watowich, Amy B Heimberger, Giulio F Draetta, Jian Hu

Faculty, Staff and Student Publications

Novel therapeutic strategies targeting glioblastoma (GBM) often fail in the clinic, partly because preclinical models in which hypotheses are being tested do not recapitulate human disease. To address this challenge, we took advantage of our previously developed spontaneous Qk/Trp53/Pten (QPP) triple-knockout model of human GBM, comparing the immune microenvironment of QPP mice with that of patient-derived tumors to determine whether this model provides opportunity for gaining insights into tumor physiopathology and preclinical evaluation of therapeutic agents. Immune profiling analyses and single-cell sequencing of implanted and spontaneous tumors from QPP mice and from patients with glioma revealed intratumoral immune components that …

Immune Landscape Of A Genetically Engineered Murine Model Of Glioma Compared With Human Glioma, Daniel B Zamler, Takashi Shingu, Laura M Kahn, Kristin Huntoon, Cynthia Kassab, Katarzyna Tomczak, Yating Li, Ivy Lai, Rocio Zorilla-Veloz, Cassian Yee, Kunal Rai, Betty Ys Kim, Stephanie S Watowich, Amy B Heimberger, Giulio F Draetta, Jian Hu

Faculty, Staff and Student Publications

Novel therapeutic strategies targeting glioblastoma (GBM) often fail in the clinic, partly because preclinical models in which hypotheses are being tested do not recapitulate human disease. To address this challenge, we took advantage of our previously developed spontaneous Qk/Trp53/Pten (QPP) triple-knockout model of human GBM, comparing the immune microenvironment of QPP mice with that of patient-derived tumors to determine whether this model provides opportunity for gaining insights into tumor physiopathology and preclinical evaluation of therapeutic agents. Immune profiling analyses and single-cell sequencing of implanted and spontaneous tumors from QPP mice and from patients with glioma revealed intratumoral immune components that …

A New Species Of Chiromachetes Pocock, 1899 (Scorpiones: Hormuridae) From Southern Western Ghats, India, Akshay Khandekar, Tejas Thackeray, Swapnil Pawar, Satpal Gangalmale, Vivek Waghe

Euscorpius

No abstract provided.

Anti-Bat Ultrasound Production In Moths Is Globally And Phylogenetically Widespread, Jesse R. Barber, Brian C. Leavell, Krystie A. Miner, Brandt Quirk-Royal

Biology Faculty Publications and Presentations

Warning signals are well known in the visual system, but rare in other modalities. Some moths produce ultrasonic sounds to warn bats of noxious taste or to mimic unpalatable models. Here, we report results from a long-term study across the globe, assaying moth response to playback of bat echolocation. We tested 252 genera, spanning most families of large-bodied moths, and document anti-bat ultrasound production in 52 genera, with eight subfamily origins described. Based on acoustic analysis of ultrasonic emissions and palatability experiments with bats, it seems that acoustic warning and mimicry are the raison d'être for sound production in most …

Market Animal Feed Efficiency: A Tool For Evaluating Feed Conversion, Cody Zesiger, Jessie Hadfield, Jacob Hadfield, Joshua Dallin

All Current Publications

The market animal feed efficiency calculator is designed to help youth livestock producers become more profitable and improve market animal feed efficiency.

Disturbance Ecology Of The Sonadora River Rock Pool Insect Assemblage At Luquillo Experimental Forest, Puerto Rico, Patrick Gilkey

Master's Theses

Freshwater rock pools can serve as habitat for aquatic insects. Flash-flooding can have profound effects on insect communities in streams and rivers, but these effects have not been studied on freshwater rock pools. The goal of this thesis was to describe ecological patterns of the Sonadora River rock pool insect community at the Luquillo Experiment Forrest, Puerto Rico, specifically in response to intense flash-flooding, and then perform experiments to elucidate possible processes to explain those patterns. The rock pools contained primarily three taxa of Dipteran (true-fly) larvae, in order of decreasing abundance, 1) a newly discovered species of ceratopogonid (biting …

The Evolution Of Honeycomb, Grace Cope

Master of Science in Integrative Biology Theses

The regular cells of honeycomb nests have fascinated scholars and mathematicians for millennia. In 36BC, the Roman scholar Marcus Terentius Varro first proposed the “honeycomb conjecture,” in which he postulated that bees build their comb using hexagons because it is the shape that requires the least amount of material to divide space into equal portions. Since this time, honeycomb has been used by engineers in applications that range from skyscraper supports to impact absorbers inside the feet of the lunar lander during the first moon missions, but mysteries remain about the biological origins of honeycomb and the mechanical properties of …

Ceramide-1-Phosphate: A Novel Regulator Of Golgi Fragmentation, Golgi-Er Vesicle Trafficking, And Anaplasma Phagocytophilum Pathogenesis, Anika Nayar Ali

USF Tampa Graduate Theses and Dissertations

Anaplasma phagocytophilum, the etiologic agent of human granulocytic anaplasmosis (HGA), is a tick transmitted obligate intracellular bacterium that infects granulocytes, bone marrow progenitor cells and endothelial cells. HGA exhibits symptoms of febrile illness including fever, chills, headache, malaise, leukopenia and elevated liver enzymes which are treatable by antibiotics. However, elderly and immunocompromised patients are at a greater risk of developing fatal complications. Inside the host cell A.phagocytophilum resides in host cell derived vacuole called A.phagocytophilum occupied vacuole (ApV) where it undergoes its biphasic lifecycle. Being an intracellular pathogen, A.phagocytophilum relies heavily on nutrient acquisition from host cell and to obtain …

Integrated Screens Uncover A Cell Surface Tumor Suppressor Gene Kirrel Involved In Hippo Pathway, Chao Wang, Xu Feng, Dan Su, Zhen Chen, Shimin Wang, Min Huang, Huimin Zhang, Siting Li, Ling Yin, Randy L Johnson, Traver Hart, Junjie Chen

Faculty, Staff and Student Publications

Cell surface proteins play essential roles in various biological processes and are highly related to cancer development. They also serve as important markers for cell identity and targets for pharmacological intervention. Despite their great potentials in biomedical research, comprehensive functional analysis of cell surface proteins remains scarce. Here, with a de novo designed library targeting cell surface proteins, we performed in vivo CRISPR screens to evaluate the effects of cell surface proteins on tumor survival and proliferation. We found that

An Investigation Into Use Of Natural Mistletoe Extracts And Their Formulations As Potential Anti-Cancer Treatments In Human Carcinomas, Rebecca Healy, Anne M. Friel

SURE Journal: Science Undergraduate Research Experience Journal

Cancer is a prevalent disease that is responsible for around 10 million deaths a year. Cases and deaths will continue to rise daily if nothing is done to find the most effective treatment. Mistletoe is an evergreen, semi-parasitic plant that grows on trees. This plant contains a number of biologically active compounds that contribute to its anti-cancer properties e.g., lectins. Mistletoe is obtained from nature and has been shown to be safe, potent, and effective in treating cancer. Herein we review the biological effects of mistletoe on cancer cells, formulations that can be used in cancer treatment and discuss the …

Advancing Physiology With Expanded Multi-Omics., Mingyu Liang, Allen W Cowley, Andrew S Greene, Aron M Geurts, Pengyuan Liu, Yong Liu, Sridhar Rao

Faculty Research 2022

No abstract provided.

Water Availability Dictates How Plant Traits Predict Demographic Rates, Alice E. Stears, Peter B. Adler, Dana M. Blumenthal, Julie A. Kray, Kevin E. Mueller, Troy W. Ocheltree, Kevin R. Wilcox, Daniel C. Laughlin

Wildland Resources Faculty Publications

A major goal in ecology is to make generalizable predictions of organism responses to environmental variation based on their traits. However, straightforward relationships between traits and fitness are rare and likely to vary with environmental context. Characterizing how traits mediate demographic responses to the environment may enhance the predictions of organism responses to global change. We synthesized 15 years of demographic data and species-level traits in a shortgrass steppe to determine whether the effects of leaf and root traits on growth and survival depended on seasonal water availability. We predicted that (1) species with drought-tolerant traits, such as lower leaf …

Robust Network Stability Of Mosquitoes And Human Pathogens Of Medical Importance, Donald A. Yee, Catherine Dean Bermond, Limarie J. Reyes-Torres, Nicole S. Fijman, Nicole A. Scavo, Joseph Nelsen, Susan H. Yee

Faculty Publications

Background: The exact number of mosquito species relevant to human health is unknown, posing challenges in understanding the scope and breadth of vector–pathogen relationships, and how resilient mosquito vector–pathogen networks are to targeted eradication of vectors.

Methods: We performed an extensive literature survey to determine the associations between mosquito species and their associated pathogens of human medical importance. For each vector–pathogen association, we then determined the strength of the associations (i.e., natural infection, lab infection, lab dissemination, lab transmission, known vector). A network analysis was used to identify relationships among all pathogens and vectors. Finally, we examined how …

Fisheries Genomics Of Snapper (Chrysophrys Auratus) Along The West Australian Coast, Andrea Bertram, David Fairclough, Jonathan Sandoval-Castillo, Chris Brauer, Anthony Fowler, Maren Wellenreuther, Luciano B. Beheregaray

Fisheries Research Articles

The efficacy of fisheries management strategies depends on stock assessment and management actions being carried out at appropriate spatial scales. This requires understanding of spatial and temporal population structure and connectivity, which is challenging in weakly structured and highly connected marine populations. We carried out a population genomics study of the heavily exploited snapper (Chrysophrys auratus) along ~2600 km of the Australian coastline, with a focus on Western Australia (WA). We used 10,903 filtered SNPs in 341 individuals from eight sampling locations to characterize population structure and connectivity in snapper across WA and to assess if current spatial …

Formulating Decade Action Plan For Green And Quality Development In Bohai Sea, Shicheng Wang, Shuguang Liu, Hongsheng Yang

Bulletin of Chinese Academy of Sciences (Chinese Version)

As the only inland sea of China, Bohai Sea has been regarded as the integrated part of the national territorial space toward green and high-quality development in the new era. The comprehensive environmental treatment in Bohai Sea has achieved noticeable progress, but the problems remain. The trend of biological and ecological degradation of Bohai Sea has not been fundamentally curbed. Guided by President Xi Jinping thought on ecological civilization and on building a maritime community with a shared future, China should join into the Implementation Plan for the United Nations Decade of Marine Science for Sustainable Development (2021-2030), and manage …