Life Sciences Commons^™

Two-Pore Channel Blockade By Phosphoinositide Kinase Inhibitors Ym201636 And Pi-103 Determined By A Histidine Residue Near Pore-Entrance, Canwei Du, Xin Guan, Jiusheng Yan

Faculty, Staff and Student Publications

Human two-pore channels (TPCs) are endolysosomal cation channels and play an important role in NAADP-evoked Ca2+ release and endomembrane dynamics. We found that YM201636, a PIKfyve inhibitor, potently inhibits PI(3,5)P2-activated human TPC2 with an IC50 of 0.16 μM. YM201636 also effectively inhibits NAADP-activated TPC2 and a constitutively-open TPC2 L690A/L694A mutant channel; whereas it exerts little effect when applied in the channel's closed state. PI-103, a YM201636 analog and an inhibitor of PI3K and mTOR, also inhibits human TPC2 with an IC50 of 0.64 μM. With mutational, virtual docking, and molecular dynamic simulation analyses, we found that YM201636 and PI-103 directly …

Molecular Profiles Of Serum-Derived Extracellular Vesicles In High-Grade Serous Ovarian Cancer, Li Zhao, Sara Corvigno, Shaolin Ma, Joseph Celestino, Nicole D Fleming, Adrian Lankenau Ahumada, Erika J Thompson, Hongli Tang, Shannon N Westin, Amir A Jazaeri, Jianhua Zhang, P Andrew Futreal, Anil K Sood, Sanghoon Lee

Faculty, Staff and Student Publications

Patients with high-grade serous ovarian cancer (HGSC) who have no visible residual disease (R0) after primary surgery have the best clinical outcomes, followed by patients who undergo neoadjuvant chemotherapy (NACT) and have a response enabling interval cytoreductive surgery. Clinically useful biomarkers for predicting these outcomes are still lacking. Extracellular vesicles (EVs) have been recognized as liquid biopsy-based biomarkers for early cancer detection and disease surveillance in other disease settings. In this study, we performed extensive molecular characterization of serum-derived EVs and correlated the findings with therapeutic outcomes in patients with HGSC. Using EV-DNA whole-genome sequencing and EV-RNA sequencing, we identified …

Physical Activity And Mindfulness Are Associated With Lower Anxiety In Different But Complementary Ways, Allison L. Mizzi, Antony D. Karelis, Jennifer J. Heisz

International Journal of Exercise Science

International Journal of Exercise Science 15(7): 1075-1084, 2022. Introduction: Anxiety is the most prevalent mental illness worldwide. Physical activity and mindfulness both reduce anxiety. The two are highly related; however, the relative association of physical activity and mindfulness on anxiety has yet to be examined. The present study aimed to evaluate the unique variance accounted for by physical activity and mindfulness on anxiety. Methods: Fifty young adults from a student population (M ± SD = 19 ± 0.2 years old; 58% female) reported their physical activity, mindfulness, and anxiety symptoms at the start of the study and reported their change …

Impact Of An Acute Bout Of Submaximal Aerobic Exercise On Circulating Leukocytes In Individuals With Spinal Cord Injury, Garett S. Jackson, Kendra R. Todd, Jan W. Van Der Scheer, Jeremy J. Walsh, Gabriel U. Dix, Kathleen A. Martin Ginis, Jonathan P. Little

International Journal of Exercise Science

International Journal of Exercise Science 15(3): 1085-1104, 2022. Individuals with spinal cord injury (SCI) may experience cardiovascular, musculoskeletal and organ function dysregulation. Sequelae include reduced catecholamine secretion and attenuated immune responses which may impact exercise-induced leukocytosis. The purpose of this study was to characterize major leukocyte subtypes following 30 minutes of acute, submaximal aerobic exercise, in line with updated international SCI exercise guidelines for adults. It was hypothesized that exercise would increase major leukocyte subtypes when compared to fasted baseline. Eight participants with SCI (incomplete n = 6; complete n = 2) completed a 30-minute bout of aerobic exercise on …

Aerobic Performance Detriments While Wearing A Face Mask Diverge Among Males And Females, Jose M. Moris, Yunsuk Koh

International Journal of Exercise Science

International Journal of Exercise Science 15(2): 1105-1116, 2022. The current study examined the impact of wearing a face mask (FM) at rest and while exercising on cardiorespiratory variables and aerobic performance between males and females. Nine males (21.4 ± 0.5 yr) and 9 females (21.8 ± 3.6 yr) performed a pulmonary function test and a graded maximal exercise test (GXT) on a treadmill with an FM and without an FM in random order. During the GXT, cardiorespiratory variables were measured at different exercise intensities (rest, 40%, 55%, 70%, 80%, 100% of VO₂max) and during recovery. Regardless of sex, …

Invasion Of Two Labidesthes Species Through The Tennessee-Tombigbee Waterway In Mississippi, Benjamin H. Chaffins, Calvin R. Rezac, Robert J. Ellwanger

Southeastern Fishes Council Proceedings

We re-identified the collections of Labidesthes sicculus at Mississippi Museum of Natural Science after a 2015 publication by Werneke and Armbruster elevated Labidesthes vanhyningi to the full species level. Following re-examination of specimens, 64% were identified as L. vanhyningi and 36% were identified as L. sicculus. During our study, a chronological review of the distribution of both species suggested bi-directional invasion through the Tennessee-Tombigbee Waterway. Prior to the connection of the two drainages, the only Labidesthes located in the Tennessee drainage was L. sicculus, where as L. vanhyningi was allopatric within the Tombigbee drainage. However, following canal construction, we …

Immune Cell-Protein Interactions In Alzheimer’S Disease, Kapur Dhami

Dissertations

Misfolded protein aggregates are one of the significant contributing factors in many neurogenerative diseases including Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and others. My PhD research project was to study various aspects of amyloid-β peptide, a 40-42-residue peptide and the primary component of the senile plaques found in Alzheimer’s disease (AD) brains. One objective of my research was to purify and characterize the intermediate Aβ42 species using an array of biophysical techniques like size exclusion chromatography, fluorescence assays, circular dichroism, cellular assays, and protein assays. The other major research thrust of my project was to study the role of microglial …

Regulated Dicing Of Pre-Mir-144 Via Reshaping Of Its Terminal Loop., Renfu Shang, Dmitry A Kretov, Scott I Adamson, Thomas Treiber, Nora Treiber, Jeffrey Vedanayagam, Jeffrey H Chuang, Gunter Meister, Daniel Cifuentes, Eric C Lai

Faculty Research 2022

Although the route to generate microRNAs (miRNAs) is often depicted as a linear series of sequential and constitutive cleavages, we now appreciate multiple alternative pathways as well as diverse strategies to modulate their processing and function. Here, we identify an unusually profound regulatory role of conserved loop sequences in vertebrate pre-mir-144, which are essential for its cleavage by the Dicer RNase III enzyme in human and zebrafish models. Our data indicate that pre-mir-144 dicing is positively regulated via its terminal loop, and involves the ILF3 complex (NF90 and its partner NF45/ILF2). We provide further evidence that this regulatory switch involves …

The Effectiveness Of Facebook As A Social Network Intervention To Increase Physical Activity In Chinese Young Adults, Patrick W.C. Lau, J. J. Wang, Lynda B. Ransdell, Lei Shi

Kinesiology Faculty Publications and Presentations

Introduction: Facebook, one of the worlds' most popular online social networks, is easy to access and cost-effective. Its use to enhance individual's physical activity (PA) participation should be examined. This research reviews the effectiveness and successful features of Facebook for influencing PA behaviors in young adults (Study 1), and empirically examines the efficacy of the features purported in increase PA via a Facebook intervention (Study 2).

Methods: In Study 1, PubMed, Medline, SPORT Discus, ERIC, and Embase were searched for articles that identified successful features and effectiveness of Facebook PA interventions published between January 2005 and February 2022. In Study …

Bbt Acoustic Alternative Top Bracing Cadd Data Set-Norev-2022jun28, Bill Hemphill

STEM Guitar Project’s BBT Acoustic Kit

This electronic document file set consists of an overview presentation (PDF-formatted) file and companion video (MP4) and CADD files (DWG & DXF) for laser cutting the ETSU-developed alternate top bracing designs and marking templates for the STEM Guitar Project’s BBT (OM-sized) standard acoustic guitar kit. The three (3) alternative BBT top bracing designs in this release are
(a) a one-piece base for the standard kit's (Martin-style) bracing,
(b) 277 Ladder-style bracing, and
(c) an X-braced fan-style bracing similar to traditional European or so-called 'classical' acoustic guitars.

The CADD data set for each of the three (3) top bracing designs includes …

Further Flattening Of A Degraded, Turbid Reef System Following A Severe Coral Bleaching Event, Andrew G. Bauman, Fraser A. Januchowski-Hartley, Aaron Teo, Peter A. Todd

Marine & Environmental Sciences Faculty Articles

Increasing incidence of severe coral bleaching events caused by climate change is contributing to extensive coral losses, shifts in species composition and widespread declines in the physical structure of coral reef ecosystems. With these ongoing changes to coral communities and the concomitant flattening of reef structural complexity, understanding the links between coral composition and structural complexity in maintaining ecosystem functions and processes is of critical importance. Here, we document the impacts of the 2016 global-scale coral bleaching event on seven coral reefs in Singapore; a heavily degraded, turbid reef system. Using a combination of field-based surveys, we examined changes in …

Estimates Of Daily Oxygen Consumption, Carbon Dioxide And Methane Emissions, And Heat Production For Beef And Dairy Cattle Using Spot Gas Sampling, C. Lee, K. A. Beauchemin, J. Dijkstra, D. L. Morris, K. Nichols, Paul J. Kononoff, D. Vyas

Department of Animal Science: Faculty Publications

A simulation study was conducted to examine accuracy of estimating daily O₂ consumption, CO₂ and CH₄ emissions, and heat production (HP) using a spot sampling technique and to determine optimal spot sampling frequency (FQ). Data were obtained from 3 experiments where daily O2 consumption, emissions of CO2 and CH4, and HP were measured using indirect calorimetry (respiration chamber or headbox system). Experiment 1 used 8 beef heifers (ad libitum feeding; gaseous exchanges measured every 30 min over 3 d in respiration chambers); Experiment 2 used 56 lactating Holstein-Friesian cows (restricted feeding; gaseous exchanges measured every 12 min …

Mir-489 Confines Uncontrolled Estrogen Signaling Through A Negative Feedback Mechanism And Regulates Tamoxifen Resistance In Breast Cancer, Mithil Soni, Ozge Saatci, Gourab Gupta, Yogin Patel, Manikanda Raja Keerthi Raja, Jie Li, Xinfeng Liu, Peisheng Xu Ph.D., Hongjun Wang, Daping Fan, Ozgur Sahin, Hexin Chen

Faculty Publications

Approximately 75% of diagnosed breast cancer tumors are estrogen-receptor-positive tumors and are associated with a better prognosis due to response to hormonal therapies. However, around 40% of patients relapse after hormonal therapies. Genomic analysis of gene expression profiles in primary breast cancers and tamoxifen-resistant cell lines suggested the potential role of miR-489 in the regulation of estrogen signaling and development of tamoxifen resistance. Our in vitro analysis showed that loss of miR-489 expression promoted tamoxifen resistance, while overexpression of miR-489 in tamoxifen-resistant cells restored tamoxifen sensitivity. Mechanistically, we found that miR-489 is an estrogen-regulated miRNA that negatively regulates estrogen receptor …

Patient Compliance With Wearing Lower Limb Assistive Devices: A Scoping Review, Ayisha Bashir, Danae Dinkel, Iraklis Pipinos, Jason Johanning, Sara Myers

Journal Articles

Objective

The aim of this scoping review was to identify information on compliance with wearing orthoses and other supportive devices, to discuss the barriers to adherence, and to suggest strategies for improvement based on these findings.

Methods

Online databases of PubMed, Web of Science, and the Cochrane Library were searched for articles about patients’ compliance with regard to lower limb assistive devices. In addition, a methodological quality control process was conducted. Studies were included if in the English language and related to compliance and adherence to the lower limb assistive device. Exclusion was based on first reading the abstract …

Orthotrichum Lyellii As An Active Moss Biomonitor: Examining The Interplay Between Ambient Pm10, Bulk Deposition And Heavy Metals In An Urban Environment, Scott Bradley Kiel

Dissertations and Theses

The importance of monitoring and preventing pollution in the environment is a globally recognized issue. Of the criteria pollutants outlined by the United States Environmental Protection Agency, particulate matter is among these least understood in relation to toxicity and most wide-spread. Many governments have employed continuous air quality monitoring networks to track ambient levels of particulate matter, but are often too widespread to capture the heterogeneity of the urban environment, especially for heavy metal deposition. In Portland, OR, the epiphytic moss Orthotrichum lyellii was successfully used as a low-cost passive biomonitor to increase the spatial resolution of pollution around the …

Replacing Fallow With Field Pea In Wheat Production Systems Across Western Nebraska, Samuel T. Koeshall, Amanda Easterly, Rodrigo Werle, Strahinja Stepanovic, Cody F. Creech

Department of Agronomy and Horticulture: Faculty Publications

Integration of field pea (Pisum sativum L.) (FP) into dryland cropping systems has increased due to ecological and economic benefits, paired with a growing market for pea-derived products. Challenges exist in the High Plains that limit the integration of crop rotations to replace fallow periods with FP in wheat (Triticum aestivum L.)- based systems. This experiment compares chemical summer fallow to FP in a fallow– wheat rotation at two locations in western Nebraska. Soil water content, soil fertility, N mineralization, FP yield, and subsequent hard red winter wheat (HWW) yields were recorded. Subsequent HWW yields were not different …

Mtorc1 And Mtorc2 Complexes Regulate The Untargeted Metabolomics And Amino Acid Metabolites Profile Through Mitochondrial Bioenergetic Functions In Pancreatic Beta Cells, Ghada A. Soliman, Rinat Abzalimov, Ye He

Advanced Science Research Center

Background: Pancreatic beta cells regulate bioenergetics efficiency and secret insulin in response to glucose and nutrient availability. The mechanistic Target of Rapamycin (mTOR) network orchestrates pancreatic progenitor cell growth and metabolism by nucleating two complexes, mTORC1 and mTORC2. Objective: To determine the impact of mTORC1/mTORC2 inhibition on amino acid metabolism in mouse pancreatic beta cells (Beta-TC-6 cells, ATCC-CRL-11506) using high-resolution metabolomics (HRM) and live-mitochondrial functions. Methods: Pancreatic beta TC-6 cells were incubated for 24 h with either: RapaLink-1 (RL); Torin-2 (T); rapamycin (R); metformin (M); a combination of RapaLink-1 and metformin (RLM); Torin-2 and metformin (TM); compared to the control. …

The Future Of Synbiotics: Rational Formulation And Design, David Fabian Gomez Gomez, Car Reen Kok, Robert Hutkins

Department of Food Science and Technology: Faculty Publications

Synbiotics, mixtures of live microbes and substrates selectively utilized by host organisms, are of considerable interest due to their ability to improve gastrointestinal health. However, formulating synbiotics remains challenging, due in part, to the absence of rational strategies to assess these products for synbiotic activities prior to clinical trials. Currently, synbiotics are formulated as either complementary or synergistic. Complementary synbiotics are made by combining probiotics and prebiotics, with each component acting independently and with the combination shown to provide a clinical health benefit. Most commercial synbiotics as well as those used in clinical trials have been of the complementary type. …

Immobilization Of A Bifidobacterial Endo-Ss-N-Acetylglucosaminidase To Generate Bioactive Compounds For Food Industry, Burcu Pekdemir, Hatice Duman, Ayşenur Arslan, Merve Kaplan, Melda Karyelioğlu, Tolgahan Özer, Hacı Mehmet Kayılı, Bekir Salih, Bethany M. Henrick, Rebbeca M. Duar, Sercan Karav

Department of Food Science and Technology: Faculty Publications

Conjugated N-glycans are considered next-generation bioactive prebiotic compounds due to their selective stimulation of beneficial microbes. These compounds are glycosidically attached to proteins through N-acetylglucosamines via specific asparagine residue (AsN-X-Ser/Thr). Certain bacteria such as Bifidobacterium longum subspecies infantis (B. infantis) have been shown to be capable of utilizing conjugated N-glycans, owing to their specialized genomic abilities. B. infantis possess a unique enzyme, Endo-ß-N-acetylglucosaminidase (EndoBI-1), which cleaves all types of conjugated N-glycans from glycoproteins. In this study, recombinantly cloned EndoBI- 1 enzyme activity was investigated using various immobilization methods: 1) adsorption, 2) entrapment-based alginate immobilization, …

Effect Of Ultraviolet Light Treatment On Microbiological Safety And Quality Of Fresh Produce: An Overview, Veerachandra K. Yemmireddy, Achyut Adhikari, Juan Moreira

School of Earth, Environmental, & Marine Sciences Faculty Publications

Fresh and fresh-cut fruits and vegetables have been associated in several foodborne illness outbreaks. Although investigations from those outbreaks reported that the contamination with pathogenic microorganisms may occur at any point in the farm to fork continuum, effective control strategies are still being widely investigated. In that direction, the concept of hurdle technology involving a sequence of different interventions have been widely explored. Among those interventions, ultraviolet (UV) light alone or in combination with other treatments such as use of organic acids or sanitizer solutions, has found to be a promising approach to maintain the microbiological safety and quality of …

Tdp1-Independent Pathways In The Process And Repair Of Top1-Induced Dna Damage, Huimin Zhang, Yun Xiong, Dan Su, Chao Wang, Mrinal Srivastava, Xu Feng, Zhen Chen, Junjie Chen

Faculty, Staff and Student Publications

Anticancer drugs, such as camptothecin (CPT), trap topoisomerase I (TOP1) on DNA and form TOP1 cleavage complexes (TOP1cc). Alternative repair pathways have been suggested in the repair of TOP1cc. However, how these pathways work with TDP1, a key repair enzyme that specifically hydrolyze the covalent bond between TOP1 catalytic tyrosine and the 3'-end of DNA and contribute to the repair of TOP1cc is poorly understood. Here, using unbiased whole-genome CRISPR screens and generation of co-deficient cells with TDP1 and other genes, we demonstrate that MUS81 is an important factor that mediates the generation of excess double-strand breaks (DSBs) in TDP1 …

Identification Of Fire-Retardant Chemical Treatments Via Instrumental Analysis, Caitlin Dillon

Discovery Day - Prescott

Fiber trace evidence is one of the most common forms of evidence found at a crime scene; these evidentiary items often have unique flame retardant chemical compositions or volatile chemical signatures. The retardant compounds can be used as an additional piece of evidence to trace a fiber from a victim to a source sample. This analytical comparison will require the use of specialized equipment available at the Prescott campus of Embry-Riddle Aeronautical University. This equipment and instrumentation includes scanning electron microscopy with energy dispersive spectroscopy (SEM/EDS), Fourier-transform infrared spectroscopy (FTIR), and gas chromatography-mass spectroscopy (GC/MS), using both liquid and volatile …

Monitoring Environmental Trends In Levels Of Influenza Virus And Sars-Cov-2 In Prescott, Az, Jade Blackert, Daniella Cowles

Discovery Day - Prescott

Every year, the Centers for Disease Control and Prevention and state health agencies collect surveillance data for cases of influenza. During the flu season of 2019, SARSCoV- 2, which causes the symptoms known as COVID-19, caused a global pandemic. In turn, the surveillance and testing data showed a dramatic drop in influenza case numbers compared to previous years. Influenza is one of the deadliest viruses in human history, so it seems unlikely that this drastic change would occur due to the emergence of a similar virus. This research is designed to show that the prevalence of influenza in the community …

Integrated Dna Copy Number And Expression Profiling Identifies Igf1r As A Prognostic Biomarker In Pediatric Osteosarcoma., Aaron M Taylor, Jiayi M Sun, Alexander Yu, Horatiu Voicu, Jianhe Shen, Donald A Barkauskas, Timothy J Triche, Julie M Gastier-Foster, Tsz-Kwong Man, Ching C Lau

Faculty Research 2022

Osteosarcoma is a primary malignant bone tumor arising from bone-forming mesenchymal cells in children and adolescents. Despite efforts to understand the biology of the disease and identify novel therapeutics, the survival of osteosarcoma patients remains dismal. We have concurrently profiled the copy number and gene expression of 226 osteosarcoma samples as part of the Strategic Partnering to Evaluate Cancer Signatures (SPECS) initiative. Our results demonstrate the heterogeneous landscape of osteosarcoma in younger populations by showing the presence of genome-wide copy number abnormalities occurring both recurrently among samples and in a high frequency. Insulin growth factor receptor 1 (IGF1R) is a …

The Biological Influence Of Stories & The Importance Of Reading Fiction, Elise N. Good, Katharine Schaab

The Kennesaw Journal of Undergraduate Research

Fictional narratives and stories have persisted throughout human history. However, perhaps due to a bias that stories offered nothing more than entertainment for the reader or perhaps that they are not useful outside of the realm of academia, the research within science academia has been lacking in literature on why these narratives have endured. Unfortunately, due to the lack of conversation across disciplines, particularly those of science and literature, this subject has not been thoroughly investigated through an interdisciplinary lens. Within this paper, the goal is to analyze the benefits of fictional narratives through biological, evolutionary, and neuropsychological perspectives. Research …

Evaluation Of Nearshore Carbonate Chemistry Within Major Navigational Inlets Of The Kristin Jacobs Coral Reef Ecosystem Conservation Area, Harrison Davis

All HCAS Student Capstones, Theses, and Dissertations

The absorption of atmospheric carbon dioxide (CO₂) by ocean surface water is causing ocean acidification (OA), a process by which the reaction between water and CO₂ changes ocean carbonate chemistry. Predictions of OA trajectories modeled by the Intergovernmental Panel on Climate Change (IPCC) are based on open ocean trends and may not accurately predict changes to variable nearshore ecosystems. The dynamic nature of nearshore ocean chemistry, and prevalence of OA vulnerable species within these ecosystems complicates the process of making accurate OA impact predictions within the coastal zone. Florida’s coral reef is a nearshore ecosystem vulnerable to …

Influence Of Strip Clearcuts, Deer Exclusion And Herbicide On Initial Sapling Recruitment In Northern Hardwood Forests, Stefan Hupperts, Christopher R. Webster, Robert Froese, Brandon Bal, Yvette L. Dickinson

Michigan Tech Publications, Part 1

Sapling recruitment in hardwood forests is often suppressed by overstory shade, interspecific competition, and browsing pressure from white-tailed deer (Odocoileus virginianus Zimmerman). In some northern hardwood stands, these three interacting factors may cause persistent recruitment failure of the dominant canopy species, sugar maple (Acer saccharum Marsh.), into the sapling size class. In this study, we compared initial (two-year) sugar maple and hophornbeam (Ostrya virginiana ((Mill.) K. Koch) seedling and sapling recruitment in strip clearcuts to strip selection cuts, with combinations of herbicide and deer exclosures, in a northern hardwood forest with limited sugar maple sapling recruitment. We found that sugar …

Author Correction: In Situ Recording Of Mars Soundscape (Nature, (2022), 605, 7911, (653-658), 10.1038/S41586-022-04679-0), S. Maurice, B. Chide, N. Murdoch, R. D. Lorenz, D. Mimoun, R. C. Wiens, A. Stott, X. Jacob, T. Bertrand, F. Montmessin, N. L. Lanza, C. Alvarez-Llamas, S. M. Angel Ph.D., M. Aung, J. Balaram, O. Beyssac, A. Cousin, G. Delory, O. Forni, T. Fouchet, O. Gasnault, H. Grip, M. Hecht, J. Hoffman, J. Laserna, J. Lasue, J. Maki, J. Mcclean, P. Y. Meslin, S. Le Mouélic, S. Le Mouélic, A. Munguira

Faculty Publications

In the version of this article initially published, the Acknowledgements statement was missing the text “Part of this research was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration (80NM0018D0004),” which has now been inserted in the HTML and PDF versions of the article.

The Acute Effect Of In Natura Beetroot Juice Intake On Intra-Session Exercise Sequences During Concurrent Training, Diego Souza, Julio Ribeiro, Andrea Simão, Millán Aguilar-Navarro, Marcos Polito

International Journal of Exercise Science

International Journal of Exercise Science 15(2): 1075-1085, 2022. The purpose of this study was to analyze the acute effects of in natura beetroot juice intake on intra-session exercise sequences during concurrent training. Following a randomized double-blind placebo-controlled crossover design, 20 well-trained men (21.4 ± 2.9 years; 74.8 ± 6.3 kg; 175.7 ± 5.0 cm) performed two concurrent training sessions with different intra-session exercise sequences: CT1 (aerobic exercise + resistance exercises) and CT2 (resistance exercises + aerobic exercise). The resistance exercises were bench-press, lat-pull down, and shoulder-press (three sets to failure; 2 s cadence for the concentric and eccentric phases; 90 …

Snp Genotyping For Purity Assessment Of A Forage Oat (Avena Sativa L.) Variety From Colombia, Luis Fernando Campuzano-Duque, Diego Bejarano-Garavito, Javier Castillo-Sierra, Daniel Ricardo Torres-Cuesta, Andrés J. Cortés, Matthew Wohlgemuth Blair

Agricultural and Environmental Sciences Faculty Research

Single nucleotide polymorphism (SNP) markers have multiple applications in plant breeding of small grains. They are used for the selection of divergent parents, the identification of genetic variants and marker-assisted selection. However, the use of SNPs in varietal purity assessment is under-reported, especially for multi-line varieties from the public sector. In the case of variety evaluation, these genetic markers are tools for maintaining varietal distinctness, uniformity and stability needed for cultivar release of multi-line or pure-line varieties of inbred crops. The objective of this research was to evaluate the purity and relationships of one original (AV-25) and two multi-line sub-populations …