Life Sciences Commons^™

Assessing Methods For Enhancing Monarch Habitat In Re-Enrolling Crp Sites, Eva Van De Mortel, Tristan Murphy, Laura L. Jackson Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

Over the last 30 years, monarch butterflies and their habitats have been in dramatic decline. Land enrolled in the Conservation Reserve Program (CRP) is one of the greatest resources available to increase monarch population as it has the largest amount of space available for monarch habitat. However, some fields have lost the nectar plants and milkweeds necessary for monarch habitat. This study, asked how to best suppress vegetation in re-enrolling CRP land so that overseeded forbs have a better chance of survival? Vegetation surveys from 6 different sites in northeastern Iowa and results from 2021 to 2022 were compared. We …

Using Environmental Dna (Edna) To Assess Pollinator Communities In Cedar Valley, Mila Haynes, Brody Jack, Ai Wen Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

Tracking biological activity in Iowa prairies can prove difficult with such high levels of biodiversity. However, using environmental DNA (eDNA) collected from prairie flowers can provide integral information about the insects that pollinate various plants.

When bees land on flowers, they shed cells and leave trace amounts of their DNA behind. After extracting the DNA from these flowers using the Qiagen DNeasy Extraction method, the samples are then mixed with B10 primer. This primer allows only the bee DNA to be targeted once the samples are put through a Polymerase Chain Reaction (PCR).

The purpose of this study is to …

Curating A Global Collection Of Grapsoid Crabs For The American Natural History Museum, Nyc, Taylor Pruisman, Amber Finke, Carl Thurman Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

Curation is a powerful tool when accessing information about specialized collections. Many researchers that use specimens borrowed from other institutions often find the supporting information insufficient. Over the last two years, a large collection of preserved grapsoid crabs was received from Frank H. Barnwell, Ecology, Evolution, and Behavior, University of Minnesota-Twin Cities.The collection of land crabs has undergone an extensive curation process done by University of Northern Iowa undergraduates. The curation process included identifying species, locating pin points, counting of sexes, rehousing and producing new labels. The specimens were sorted by their species, counted by gender, and their collection locations …

Potential Psychoactive Effects Of Microalgal Bioactive Compounds For The Case Of Sleep And Mood Regulation: Opportunities And Challenges, Bozena Mccarthy, Graham O’Neill Dr, Nissreen Abu-Ghannam Prof.

Articles

Sleep deficiency is now considered an emerging global epidemic associated with many serious health problems, and a major cause of financial and social burdens. Sleep and mental health are closely connected, further exacerbating the negative impact of sleep deficiency on overall health and well-being. A major drawback of conventional treatments is the wide range of undesirable side-effects typically associated with benzodiazepines and antidepressants, which can be more debilitating than the initial disorder. It is therefore valuable to explore the efficiency of other remedies for complementarity and synergism with existing conventional treatments, leading to possible reduction in undesirable side-effects. This review …

Arsr Family Regulator Msmeg_6762 Mediates The Programmed Cell Death By Regulating The Expression Of Hnh Nuclease In Mycobacteria, Xiangke Duan, Xue Huang, Junqi Xu, Xue Li, Jingjing Niu, Xiaoli Du, Xiaoyu Wang, Jiang Li, Michael Kelly, Jiaohan Guo, Ke Zhang, Yu Huang, Biao Kan, Jianping Xie

Biology Student Work

Programmed cell death (PCD) is the result of an intracellular program and is accomplished by a regulated process in both prokaryotic and eukaryotic organisms. Here, we report a programed cell death process in Mycobacterium smegmatis, an Actinobacteria species which involves a transcription factor and a DNase of the HNH family. We found that over-expression of an ArsR family member of the transcription factor, MSMEG_6762, leads to cell death. Transcriptome analysis revealed an increase in the genes’ transcripts involved in DNA repair and homologous recombination, and in three members of HNH family DNases. Knockout of one of the DNase genes, MSMEG_1275, …

Sex Differences In The Genetic Architecture Of Cognitive Resilience To Alzheimer's Disease., Jaclyn M Eissman, Logan Dumitrescu, Emily R Mahoney, Alexandra N Smith, Shubhabrata Mukherjee, Michael L Lee, Phoebe Scollard, Seo Eun Choi, William S Bush, Corinne D Engelman, Qiongshi Lu, David W Fardo, Emily H Trittschuh, Jesse Mez, Catherine C Kaczorowski, Hector Hernandez Saucedo, Keith F Widaman, Rachel F Buckley, Michael J Properzi, Elizabeth C Mormino, Hyun Sik Yang, Theresa M Harrison, Trey Hedden, Kwangsik Nho, Shea J Andrews, Douglas Tommet, Niran Hadad, R Elizabeth Sanders, Douglas M Ruderfer, Katherine A Gifford, Xiaoyuan Zhong, Neha S Raghavan, Badri N Vardarajan, Margaret A Pericak-Vance, Lindsay A Farrer, Li San Wang, Carlos Cruchaga, Gerard D Schellenberg, Nancy J Cox, Jonathan L Haines, C Dirk Keene, Andrew J Saykin, Eric B Larson, Reisa A Sperling, Richard Mayeux, Michael L Cuccaro, David A Bennett, Julie A Schneider, Paul K Crane, Angela L Jefferson, Timothy J Hohman

Faculty Research 2022

Approximately 30% of elderly adults are cognitively unimpaired at time of death despite the presence of Alzheimer's disease neuropathology at autopsy. Studying individuals who are resilient to the cognitive consequences of Alzheimer's disease neuropathology may uncover novel therapeutic targets to treat Alzheimer's disease. It is well established that there are sex differences in response to Alzheimer's disease pathology, and growing evidence suggests that genetic factors may contribute to these differences. Taken together, we sought to elucidate sex-specific genetic drivers of resilience. We extended our recent large scale genomic analysis of resilience in which we harmonized cognitive data across four cohorts …

Qtlviewer: An Interactive Webtool For Genetic Analysis In The Collaborative Cross And Diversity Outbred Mouse Populations, Matthew J Vincent, Isabela Gerdes Gyuricza, Gregory R Keele, Daniel Mario Gatti, Mark P. Keller, Karl W. Broman, Gary Churchill

Faculty Research 2022

The Collaborative Cross and the Diversity Outbred mouse populations are related multiparental populations, derived from the same 8 isogenic founder strains. They carry >50¬†M known genetic variants, which makes them ideal tools for mapping genetic loci that regulate phenotypes, including physiological and molecular traits. Mapping quantitative trait loci requires statistical and computational training, which can present a barrier to access for some researchers. The QTLViewer software allows users to graphically explore Collaborative Cross and Diversity Outbred quantitative trait locus mapping and related analyses performed through the R/qtl2 package. Additionally, the QTLViewer website serves as a repository for published Collaborative Cross …

A Review Of Host Plants For The Tortricid Tribe Grapholitini, With A Synopsis Of Host Utilization By Genus (Lepidoptera: Tortricidae), John W. Brown

Insecta Mundi

A database of larval host plants for the tortricid tribe Grapholitini (Lepidoptera: Tortricidae: Ole­threutinae) is presented, and larval hosts are summarized for each genus. Food plants have been reported for over 400 of the approximately 1,644 described species of Grapholitini. Of the 81 genera currently as­signed to the tribe, at least one larval host has been reported for 51. Ninety-seven different plant families have been reported at least once for a species of Grapholitini, with the greatest number of grapholitines recorded from Fabaceae (168 species), followed by Fagaceae (43 species), Pinaceae (43), Sapindaceae (36), Rosaceae (30), Asteraceae (30), Euphorbiaceae (15), …

Whiteflies (Hemiptera: Aleyrodidae) Intercepted On Plant Product Imported To South Korea From 2013–2021, Suhyeon Lee, Soo-Jung Suh

Insecta Mundi

Current globalization and technological progress has facilitated and increased the international trade of plant products worldwide and has promoted the long-distance movement of immobile sucking pests such as whiteflies attached on plants. Therefore, being able to compile and update information on intercepted insect pests will help to improve the inspection procedures, to detect, identify and mitigate the damage caused by exotic invasive pests. Records of whiteflies (Hemiptera: Aleyrodidae) intercepted on import plants from 2013 to 2021 in the Pest Information System (PIS) database of South Korea were analyzed. A total of 32 species belonging to 19 genera were intercepted on …

Landscape Tree Irrigation Calculator, Shital Poudyal

All Current Publications

Trees, undoubtedly, are the most valuable plant in a landscape and must be prioritized for irrigation in drought conditions. Grass easily recovers from a period of long drought; hence, it must be placed last on the priority list for irrigation. Many resources are available online that explain ways to irrigate landscape trees. However, growers and homeowners in Utah are still confused about quantifying and applying the appropriate amount of water a landscape tree needs. This Excel spreadsheet is an irrigation calculator designed to determine the amount of water a landscape tree needs in Utah’s hot and dry summer months. Find …

Slamming Dynamics Of Diving And Its Implications For Diving-Related Injuries, Anupam Pandey, Jisoo Yuk, Brian Chang, Frank E. Fish, Sunghwan Jung

Biology Faculty Publications

In nature, many animals dive into water at high speeds, e.g., humans dive from cliffs, birds plunge, and aquatic animals porpoise and breach. Diving provides opportunities for animals to find prey and escape from predators and is a source of great excitement for humans. However, diving from high platforms can cause severe injuries to a diver. In this study, we demonstrate how similarity in the morphology of diving fronts unifies the slamming force across diving animals and humans. By measuring a time-averaged impulse that increases linearly with the impact height, we are able to estimate the unsteady hydrodynamic forces that …

Reviving Ghost Alleles: Genetically Admixed Coyotes Along The American Gulf Coast Are Critical For Saving The Endangered Red Wolf, Bridgett M. Vonholdt, Joseph W. Hinton, Amy C. Shutt, Sean M. Murphy, Melissa L. Karlin, Jennifer R. Adams, Lisette P. Waits, Kristin Brzeski

Michigan Tech Publications, Part 1

The last known red wolves were captured in southwestern Louisiana and eastern Texas in 1980 to establish a captive breeding population. Before their extirpation, gene flow with coyotes resulted in the persistence of endangered red wolf genetic variation in local coyote populations. We assessed genomic ancestry and morphology of coyotes in southwestern Louisiana. We detected that 38 to 62% of the coyote genomes contained red wolf ancestry acquired in the past 30 years and have an admixture profile similar to that of the canids captured before the extirpation of red wolves. We further documented a positive correlation between ancestry and …

Quantification And Machine Learning Based N2o–N And Co2–C Emissions Predictions From A Decomposing Rye Cover Crop, Deepak R. Joshi, David E. Clay, Sharon A. Clay, Janet Moriles-Miller, Aaron L. M. Daigh, Graig Reicks, Shaina Westhoff

Department of Agronomy and Horticulture: Faculty Publications

Cover crops improve soil health and reduce the risk of soil erosion. However, their impact on the carbon dioxide equivalence (CO_2e) is unknown. Therefore, the objective of this 2-yr study was to quantify the effect of cover crop-induced differences in soil moisture, temperature, organic C, and microorganisms on CO_2e, and to develop machine learning algorithms that predict daily N₂O–N and CO₂–C emissions. The prediction models tested were multiple linear regression, partial least square regression, support vector machine, random forest (RF), and artificial neural network. Models’ performance was accessed using R², …

Connecting Above- And Belowground Effects Of Climate Warming On Bumble Bee Health, Francis Mullan

Master of Science in Integrative Biology Theses

Climate warming has been identified as one of the primary factors causing worldwide pollinator declines. One of the most at-risk groups of pollinators is bumble bees (Bombus spp.). Bumble bees are common, widespread, and key pollinators of a wide range of crops and wild plants. Although studies have examined the thermal physiological traits of individual bumble bees to understand how they may be impacted by climate warming, little to no studies have examined how climate warming may impact whole bumble bee colonies both in their ability to thermoregulate their nest for their brood or in their ability to forage …

Exploring New Methods For Quantifying Soil Reduction, Redox Potential, And Iron Reducing Bacteria Using The Iris Method In Wetland Soils., Jessica Everett

Biology Theses

The presence of hydric soils is one of the key factors used to identify and delineate wetlands as specified by the United States Fish and Wildlife Service (USFWS). The Indicator of Reduction in Soil (IRIS) Method, accepted by the National Technical Committee for Hydric Soils (NTCHS) can indicate iron reduction in soil which can identify hydric soil for wetland delineation efforts. This thesis was conducted to see if the IRIS method had potential for other uses such as quantifying relative measurements of iron reducing bacteria and to see if a colorimetric scale could be curated to bring less subjection to …

Synopsis Of The Tribe Amarotypini In New Zealand (Coleoptera: Carabidae), André Larochelle, Marie-Claude Larivière

Insecta Mundi

The tribe Amarotypini (Coleoptera: Carabidae: Migadopinae) is revised for New Zealand. Three genera and fourteen species are recognized.

Two genera and thirteen species are described as new: Amarophilus Larochelle and Larivière new genus, Amarophilus lomondensis Larochelle and Larivière new species, Amarophilus otagoensis Larochelle and Larivière new species, Amarophilus rotundicollis Larochelle and Larivière new species, Amarophilus wanakensis Larochelle and Larivière new species, Amarotypus fiordlandensis Larochelle and Larivière new species, Amarotypus glasgowensis Larochelle and Larivière new species, Amarotypus murchisonorum Larochelle and Larivière new species, Amarotypus simoninensis Larochelle and Larivière new species, Amarotypus takaheensis …

A New Phyllophaga (Listrochelus) Timida Group Species From Baja California Sur (Coleoptera: Scarabaeidae: Melolonthinae), William B. Warner

Insecta Mundi

Phyllophaga (Listrochelus) baja, new species (Coleoptera: Scarabaeidae: Melolonthinae), is de­scribed and illustrated. Its relationship to other species in the “timida group” is discussed.

Morón (2002) reviewed the Phyllophaga Harris (Listrochelus Blanchard) species near P. timida (Horn) and included a key to the eight known species of the “timida group.” Many species in this group are difficult to dis­tinguish from one another via external characters; however, the male genital shape is diagnostic for each. Here I describe a new species from Baja California Sur, Mexico, that falls within that group.

New Records Of Invasive Aphids (Hemiptera: Aphididae) On Garlic Mustard In The Usa, Doris Lagos-Kutz, Rebecah Troutman, Glen L. Hartman

Insecta Mundi

Lipaphis alliariae Müller (Hemiptera: Aphididae) was identified from specimens collected in Lake County, Ohio, on the invasive garlic mustard, Alliaria petiolata (Bieb.) Cavara & Grande (Brassicaceae). The identification was performed on apterous viviparae using morphological and molecular data. Body color and shape of the cauda discriminated L. alliariae and L. pseudobrassicae Davis. Measurements of morphological characters of both species were similar, but they have distinct characters distinguishing them from L. erysimi Kaltenbach. Neighbor-joining analysis of cytochrome oxidase 1 (Cox1) barcoding indicated a close relation­ship of the aphids that feed on Brassicaceae, and the range of pair-wise distances for Cox1 barcoding …

Natural History Of Cassida Sphaerula Boheman, 1854 (Coleoptera: Chrysomelidae: Cassidinae: Cassidini) On Arctotheca Prostrata (Salisb.) Britten (Asteraceae: Arctotidinae) In South Africa, With A Checklist Of South African Cassidinae (Leaf-Mining And Tortoise Beetles), Sally Adam, Mariana Campos, Hugh D.C. Heron, C. L. Staines, Rob Westerduijn, Caroline Simmrita Chaboo

Insecta Mundi

The tortoise beetle, Cassida sphaerula Boheman, 1854 (Coleoptera: Chrysomelidae: Cassidinae: Cassidini) is endemic to South Africa. Its endemic host, Arctotheca prostrata (Salisb.) Britten (Asteraceae) has been introduced in other countries where it is becoming invasive. Cassida sphaerula could provide a potential biocontrol of Arctotheca weeds as it spends the entire life cycle on this host. An intensive field study, with rearing, photography, and short films of C. sphaerula was conducted in its native habitat to document the life cycle. A checklist of Cassidinae genera in South Africa, along with 19 new host records for Cassidini species in South Africa are …

Ppp1r7 Is A Novel Translocation Partner Of Cbfb Via T(2; 16)(Q37; Q22) In Acute Myeloid Leukemia, Lulu Wang, Wei Wang, Hannah C Beird, Xueqian Cheng, Hong Fang, Gokce A Toruner, M James You, Ghayas C Issa, Gautam Borthakur, Guang Peng, Joseph D Khoury, L Jeffrey Medeiros, Zhenya Tang

Faculty, Staff and Student Publications

In a subset of acute myeloid leukemia (AML) cases, the core binding factor beta subunit gene (CBFB) was rearranged via inv(16)(p13.1q22) or t(16;16)(p13.1;q22), in which the smooth muscle myosin heavy chain 11 gene (MYH11) was the partner (CBFB::MYH11). Rare variants of CBFB rearrangement occurring via non-classic chromosomal aberrations have been reported, such as t(1;16), t(2;16), t(3;16), t(5;16), and t(16;19), but the partners of CBFB have not been characterized. We report a case of AML with a complex karyotype, including t(2;16)(q37;q22), in which the protein phosphatase 1 regulatory subunit 7 gene (PPP1R7) at …

Clinical Outcomes Of Patients With Recurrent Microsatellite-Stable Endometrial Cancer In Early-Phase Immunotherapy Clinical Trials, Jeffrey A How, Amir A Jazaeri, Siqing Fu, Jordi Rodon Ahnert, Jing Gong, Hanna Ferreira Dalla Pria, Amber Johnson, Ying Yuan, Funda Meric-Bernstam, Aung Naing

Faculty, Staff and Student Publications

Recurrent microsatellite stable (MSS) endometrial cancer has poor response to conventional therapy and limited efficacy with immune checkpoint monotherapy. We conducted a retrospective study of recurrent MSS endometrial cancer patients enrolled in immunotherapy-based clinical trials at MD Anderson Cancer Center between 1 January 2010 and 31 December 2019. Patients were evaluated for radiologic response using RECIST 1.1 criteria, progression-free survival (PFS), and overall survival (OS). Thirty-five patients were treated with immune checkpoint inhibitors: 8 with monotherapy, 17 with immunotherapy (IO) in combination with another IO-only, and 10 with IO in combination with non-IO therapy. Among those treated with combination IO …

Protein Shape Sampled By Ion Mobility Mass Spectrometry Consistently Improves Protein Structure Prediction, Micah T. Donor, Sm Bargeen Alam Turzo, Justin T. Seffernick, Amber D. Rolland, Stein Heinze, James S. Prell, Vicki H. Wysocki, Steffen Lindert

Faculty Publications - Department of Biological & Molecular Science

Ion mobility (IM) mass spectrometry provides structural information about protein shape and size in the form of an orientationally-averaged collision cross-section (CCSIM). While IM data have been used with various computational methods, they have not yet been utilized to predict monomeric protein structure from sequence. Here, we show that IM data can significantly improve protein structure determination using the modelling suite Rosetta. We develop the Rosetta Projection Approximation using Rough Circular Shapes (PARCS) algorithm that allows for fast and accurate prediction of CCSIM from structure. Following successful testing of the PARCS algorithm, we use an integrative modelling approach to utilize …

The Role Of Nest Location On The Reproductive Success Of Piping Plovers Breeding At Rockaway Beach, New York, Clara I.D. Arndtsen

Theses and Dissertations

The study’s goal was to learn what aspects of the piping plover experience affect reproductive outcomes at Rockaway Beach Endangered Species Nesting Area. Nest location was found to help predict reproductive success. Nests further from American oystercatcher nests were likely to fledge more chicks. Reproductive success was low in 2021.

Limited Effects Of Precipitation Manipulation On Soil Respiration And Inorganic N Concentrations Across Soil Drainage Classes In Northern Minnesota Aspen Forests, Anna B. Stockstad, Robert A. Slesak, Alan J. Toczydlowski, Charles R. Blinn, Randall K. Kolka, Stephen D. Sebestyen

Aspen Bibliography

It is critical to gain insight into the responses of forest soils to the changing climate. We simulated future climate conditions with growing season throughfall reduction (by 50%) and winter snow removal using a paired-plot design across a soil drainage class gradient at three upland, Populus-dominated forests in northern Minnesota, USA. In situ bulk soil respiration and concentrations of extractable soil N were measured during the summers of 2020–2021. Soil respiration and N concentrations were not affected by throughfall reduction and snow removal, which was largely attributed to the limited treatment effects on soil moisture content and soil temperature. …

How Much Water Do Landscape Trees Require In Utah? An Irrigation Calculator, Shital Poudyal

All Current Publications

Trees, undoubtedly, are the most valuable plant in a landscape and must be prioritized for irrigation in drought conditions. Grass easily recovers from a period of long drought; hence, it must be placed last on the priority list for irrigation. Many resources are available online that explain ways to irrigate landscape trees. However, growers and homeowners in Utah are still confused about quantifying and applying the appropriate amount of water a landscape tree needs. This fact sheet includes a simple calculator designed to determine the amount of water a landscape tree needs in Utah’s hot and dry summer months.

Registration Of Lcs ‘Valiant’ Hard Red Winter Wheat, P. Stephen Baenziger, S. D. Masterson, J. D. Boehm Jr., Vikas Belamkar, M. D. Barnett, Devin J. Rose, Lan Xu, Stephen N. Wegulo, T. Regassa, Amanda Easterly, Cody F Creech, Dipak K. Santra, G. R. Kruger, G. W. Hergert, R. N. Klein, J. Kolmer, J. Kolmer, M.-S. Chen, Gary L. Hein, R. L. Bowden, M. J. Guttieri, G. Bai, El-Basyoni Salah, J. Poland

Department of Agronomy and Horticulture: Faculty Publications

Historically cultivars developed jointly by the University of Nebraska-Lincoln and USDA-ARS wheat (Triticum aestivum L.) improvement project tend to be late and better adapted to the northern Great Plains. LCS ‘Valiant’ (Reg. no. CV-1196, PI 693223; tested as NE10478-1) was released based on the merits of its earliness, agronomic performance, relevant disease resistances, and end-use quality characteristics and its adaptation to the central Great Plains. As such, the line was licensed to Limagrain Cereal Seeds for their ability to market outside of Nebraska. LCS Valiant hard red winter wheat was released in March 2020 by the developing institutions and …

Application In Medicine: Has Artificial Intelligence Stood The Test Of Time, Mir Ibrahim Sajid, Shaheer Ahmed, Usama Waqar, Javeria Tariq, Mohsin Chundrigar, Samira Shabbir Balouch, Sajid Abaidullah

Medical College Documents

Artificial intelligence (AI) has proven time and time again to be a game-changer innovation in every walk of life, including medicine. Introduced by Dr. Gunn in 1976 to accurately diagnose acute abdominal pain and list potential differentials, AI has since come a long way. In particular, AI has been aiding in radiological diagnoses with good sensitivity and specificity by using machine learning algorithms. With the coronavirus disease 2019 pandemic, AI has proven to be more than just a tool to facilitate healthcare workers in decision making and limiting physician-patient contact during the pandemic. It has guided governments and key policymakers …

Stabilization By Adaptive Feedback Control For Positive Difference Equations With Applications In Pest Management, J. Edholm, C. Guiver, Richard Rebarber, Brigitte Tenhumberg, S. Townley

School of Biological Sciences: Faculty Publications

An adaptive feedback control scheme is proposed for stabilizing a class of forced nonlinear positive difference equations. The adaptive scheme is based on so-called high-gain adaptive controllers and contains substantial robustness with respect to model uncertainty as well as with respect to persistent forcing signals, including measurement errors. Our results take advantage of the underlying positive systems structure and ideas from input-to-state stability from nonlinear control theory. Our motivating application is to pest or weed control, and in this context the present work substantially strengthens previous work by the authors. The theory is illustrated with examples.

Chemical And Biological Stressors Threaten Native Fish Diversity In The Spring River Subbasin Of Kansas, Alexandra King

Electronic Theses & Dissertations

Chemical pollution and nonnative species are two of the major threats facing freshwater fishes. As such, understanding how freshwater fish communities respond to pollution remediation efforts and nonnative fish introductions are primary goals of native fish conservation. Within the Spring River subbasin (SRS) of southeastern Kansas, this study examined the long-term response of riffle fish communities to decreases in heavy metal concentrations, as well as the effects of introduced Blackspotted Topminnow (Fundulus olivaceus) on native Blackstripe Topminnow (Fundulus notatus). The objectives of our research in Chapter 01 of this thesis were to quantify changes in riffle …

The Lupus Susceptibility Allele Drb1*03:01 Encodes A Disease-Driving Epitope, Bruna Miglioranza Scavuzzi, Vincent Van Drongelen, Bhavneet Kaur, Jennifer Callahan Fox, Jianhua Liu, J Michelle Kahlenberg, Fernando Benavides, Frederick W Miller, Amr H Sawalha, Joseph Holoshitz

Faculty, Staff and Student Publications

The HLA-DRB1*03:01 allele is a major genetic risk factor in systemic lupus erythematosus (SLE), but the mechanistic basis of the association is unclear. Here we show that in the presence of interferon gamma (IFN-γ), a short DRB1*03:01-encoded allelic epitope activates a characteristic lupus transcriptome in mouse and human macrophages. It also triggers a cascade of SLE-associated cellular aberrations, including endoplasmic reticulum stress, unfolded protein response, mitochondrial dysfunction, necroptotic cell death, and production of pro-inflammatory cytokines. Parenteral administration of IFN-γ to naïve DRB1*03:01 transgenic mice causes increased serum levels of anti-double stranded DNA antibodies, glomerular immune complex deposition and histopathological renal …