Life Sciences Commons^™

Example Imagery Survey Data And Project Files To Test Wisdam Software, Amanda Hodgson, Christophe Cleguer

Research Datasets

This example dataset is designed to support our WISDAM (Wildlife Image Survey Detection and Mapping) software—a free, open‑source application (available at www.WISDAMapp.org) created for researchers conducting wildlife imagery surveys. WISDAM enables users to generate data suitable for abundance estimation and spatial modelling from aerial photographic surveys of marine and terrestrial megafauna.

Hydroponics In The Classroom: Cultivating Plants, Captivating Young People, Stacy A. Adams, Christian Stephenson, Theresa James, Kim Todd

Department of Agronomy and Horticulture: Faculty Publications

Few students take the initiative to pursue coursework beyond that required for progression to graduation. Limited are opportunities that connect educational content to industry application, potentially inspiring young people towards impactful careers. The United States government has emphasized the importance of STEM education to ensure national security and to maintain economic competitiveness globally. STEM education is not a defined discipline but an approach for building knowledge through application and engagement. Developing and managing a hydroponics prototype provides numerous opportunities for embedding STEM content into a fun and interactive project. This poster provides inspiration for constructing a simple deep water culture …

Bioprinted Scaffolds For Investigating Fibroblast Alignment, Lillian Thro, Daeha Joung Ph.D.

Undergraduate Research Posters

Fibroblasts play a vital role in tissue repair, ECM remodeling, and maintaining structural integrity in human tissues. However, their behavior in engineered 3D microenvironments remains poorly understood. Traditional 2D cultures fail to capture the complex cell-cell and cell-matrix interactions that govern fibroblast alignment, migration, and proliferation in vivo. To address this gap, this project uses bioprinted scaffolds that mimic native ECM structures and provide a platform for studying fibroblast behavior in controlled microenvironments. The project focuses on varying scaffold channel properties—such as width, depth, and geometry—to investigate how physical cues influence fibroblast organization and collective migration. A biodegradable gelatin-based ECM …

A Novel 3d Imaging Approach To Study Evolutionary Innovations In Solanaceae Inflorescences, Devin L. Jackson, Joonseog Kim, Wenheng Zhang Dr.

Undergraduate Research Posters

Understanding the relatedness of angiosperms and the evolution of their inflorescence remains challenging, as these structures are highly modified and prone to convergent evolution. The current research describing inflorescence architecture, whether genetically or morphologically focused, typically relies on text based explanations, 2D images, or 3D based models created with CAD modeling software. This creates a disparity in the reader's understanding of these models since descriptions rely heavily on the author's interpretation of the inflorescence. The goal of our study is to bridge this disconnection by producing anatomically and color-correct 3D inflorescence models using Solanaceae flowers that readers can explore directly, …

Effects Of Particle Size Difference In Two Different Sweet Bran Products On Total Tract Digestibility And Rumen Ph, Emily K. Hoefling, Anna Kobza, Jessica L. Miller, Maggie E. Youngers, Galen E. Erickson, Jim C. Macdonald

Nebraska Beef Cattle Reports

Summary with Implications

Seven ruminally fistulated heifers were utilized in a 6 x 7 row-column design to evaluate two different Sweet Bran particle size products (1.8 or 2.5 mm) included at 13%, 18%, or 23% of the diet (DM) on total tract digestibility and rumen fermentation parameters. The treatment design was a 2 x 3 factorial. Rumen pH was reduced and organic matter digestibility tended to be reduced with the small particle size when Sweet Bran was included at 13% of the diet. Both NDF digestibility and dietary digestible energy concentration tended to improve with the larger particle size. The …

Carcass Weight Influences Chilling Rate, Postmortem Metabolism, And Beef Color, Grace C. Johnson, Mariane Beline, Leila G. Venzor, Kara A. Reynolds, Gary A. Sullivan, David E. Gerrad, Jordan C. Wicks

Nebraska Beef Cattle Reports

Summary with Implications

This study investigated the relationship between carcass weight and the development of atypical dark cutting (ATDC) beef by examining differences in chilling rates, muscle metabolism, and meat quality traits. Forty-four beef carcasses (heavy weight, HW, 510.17 ± 4.6 lb.; light weight, LW, 399.21 ± 4.6 lb.) were monitored postmortem for temperature, pH, and metabolite levels. LW carcasses cooled faster from 6 to 24 hours postmortem and exhibited higher pH at 12 hours, darker lean, and lower tenderness compared to HW carcasses. Elevated levels of glycogen and glucose-6-phosphate were observed in LW carcasses, suggesting that faster chilling may …

Effect Of Nitrite Source & Reducing Compounds On Cured Color Development In An All-Beef Model System, Leila G. Venzor, Grace C. Johnson, Ashley A. Hahn, Benjamin L. Weinandt, Jordan C. Wicks, Gary A. Sullivan

Nebraska Beef Cattle Reports

Summary with Implications

This study evaluated the effects of nitrite source (sodium nitrite, celery powder, beet powder) and reducing agents (none, sodium erythorbate, acerola) on cured color development, cured meat pigment formation, and residual nitrite in an all-beef emulsion as a model system for frankfurters and smoked sausage. Treatments were prepared and analyzed for color, pigment concentration, and nitrite retention. Nitrite source did not significantly affect cured pigment or residual nitrite. The inclusion of reducing compounds significantly increased cured pigment and redness in cooked samples while lowering residual nitrite. No treatment differences were found for initial raw color. These results …

Evaluation Of Herddogg Remote Sensing Tag On Detection Of Morbidity Of Newly Received Steers, Lauryn O. Smidt, Rebecca Mcdermott, Jessica L. Sperber, Josh R. Benton, Galen E. Erickson, Jim C. Macdonald

Nebraska Beef Cattle Reports

Summary with Implications

A receiving study was conducted to evaluate the HerdDogg remote sensing tag for the detection of morbidity in newly received steers. Treatments were assigned to cattle in the order they came through the chute, and those included a treatment whereby pen-riders evaluated the cattle and made the decision on when to treat (Pen-Rider), compared to the HerdDogg remote sensing ear tag that solely identified and flagged cattle in need of treatment (TAG). Cattle were fed for 41 days before being weighed off the receiving trial. While no differences in performance were observed, the TAG treatment had a …

Use Of Virtual Fences To Co-Fence Two Groups Of Cattle With Adjacent Virtual Boundaries, Thomas E. Aquino, Mary E. Drewnoski, Biquan Zhao, Pedro Henrique Jota Fernandes, Isaque F. Vicci, Mitchell B. Stephenson, Yijie Xiong

Nebraska Beef Cattle Reports

Summary with Implications

While virtual fencing (VF) has proven effective in managing cattle, limited data exist on using it to manage two separate groups of cattle within the same area. If successful, this approach could reduce infrastructure costs and increase management flexibility. In this trial, VF-trained steers (n = 39) were placed in pastures with either no neighboring cattle (isolated) or next to a separate group with only a VF boundary between them (co-fenced). Containment exceeded 99% across all cattle, demonstrating that VF creates effective boundaries regardless of fence treatment. There was no statistical difference in the number of audio …

Application Of High-Pressure Processing Lightens The Color Of Cow Tenderloins, Kara A. Reynolds, Leila G. Venzor, Ashley A. Hahn, Grace C. Johnson, Jordan C. Wicks, Mary-Grace C. Danao, Ranjith Ramanathan, Gary A. Sullivan

Nebraska Beef Cattle Reports

Summary with Implications

This study evaluated the impact of high-pressure processing (HPP) on color, oxidative stability, and tenderness of tenderloins (psoas major) from cull cows to determine if HPP could enhance their marketability. Forty cull cow tenderloins were treated at two different HPP pressures and two different holding times, then assessed for instrumental and visual color, lipid oxidation, and Warner-Bratzler shear force during a 7-day stimulated shelf period. HPP increased initial lightness, especially at the highest pressure and longest holding time and improved redness initially. However, redness declined, and discoloration increased over the 7-day period, particularly after processing at higher …

Using High-Pressure Processing To Improve Dark-Cutting Beef Color In Tenderloins, Ashley A. Hahn, Leila G. Venzor, Shelley A. Curry, Grace C. Johnson, Mary-Grace C. Danao, Ranjith Ramanathan, Gary A. Sullivan, Jordan C. Wicks

Nebraska Beef Cattle Reports

Summary with Implications

Dark-cutting beef is a quality defect that negatively impacts consumer appeal and affects the carcass value for both producers and meat processors. These economic losses stem from poor visual appearance and reduced consumer appeal in the retail setting. Notably, different muscles within a carcass exhibit varying degrees of color stability which can influence how dark-cutting manifests across cuts. Therefore, this study aimed to examine the effect of high-pressure processing (HPP) on dark-cutting color-labile muscle. Dark-cutting and normal tenderloins were collected and portioned into two equal sections. Dark-cutting tenderloins were assigned one of 3 treatments: No HPP, 300 …

Defining The Parameters Of High-Pressure Processing To Improve The Quality Of Dark-Cutting Beef, Ashley A. Hahn, Leila G. Venzor, Shelley A. Curry, Grace C. Johnson, Mary-Grace C. Danao, Ranjith Ramanathan, Jordan C. Wicks, Gary A. Sullivan

Nebraska Beef Cattle Reports

Summary with Implications

Dark-cutting beef is a result of preharvest stress and devalues the resulting carcass. High-pressure processing (HPP) has been recently identified as a potential strategy to improve the undesirable appearance of dark-cutting beef. Dark-cutting beef strip loins were collected from a local processing facility, sectioned, and assigned an HPP pressure (300 or 450 MPa) and holding time (0, 1, 30, 60, or 90 s). Overall, HPP increased the lightness and redness of dark-cutting beef, particularly the longer the pressure was held at both pressure levels. Additionally, negative effects such as increased meat discoloration, decreased tenderness, and increased rancidity …

Evaluating Sorghum X Sudangrass Seed Digestibility, Evan Blank, Thomas E. Aquino, Mary E. Drewnoski

Nebraska Beef Cattle Reports

Summary with Implications

A sorghum-sudangrass hybrid was swathed in the hard dough stage and grazed by growing steers from November 2023 to January 2024. Steers appeared to select the seedheads when grazing. The seed made up approximately 12.6% of swathed forage mass, but its energy contribution appears limited due to poor digestibility. When incubated whole, only 23% of dry matter and 13% of starch was digestible. Grinding improved digestibility but still left 40% of the dry matter and over 85% of starch unavailable. Weathering over two months did not improve digestibility, indicating the seed coat remains intact through winter. Compared …

Long-Term Trends Of Annual Herbaceous Expansion In The Nebraska Panhandle: Rangeland Analysis Platform Data, Karley Dieckmann, Mitchell B. Stephenson, Miranda Mueller

Nebraska Beef Cattle Reports

Summary with Implications

This descriptive analysis evaluated changes in Annual Forb and Grass (AFG) herbaceous cover in the Nebraska Panhandle from 1986 to 2023 using the Rangeland Analysis Platform (RAP). It was hypothesized that AFG herbaceous cover would show an increasing trend over time, indicating the potential expansion of the annual invasive cheatgrass (Bromus tectorum). County-level RAP data were used to calculate long-term means as an indicator. Results showed an increase in AFG cover across the region. Trends were cyclical, with cover peaking and dipping over time, but the overall trajectory showed increases in AFG in the Panhandle. The increasing …

Impact Of Feeding Palm Oil To Finishing Cattle On Performance, Carcass, And Meat Characteristics, Tate S. Johnson, Rebecca L. Mcdermott, Josh R. Benton, Galen E. Erickson, Jessie C. Morrill, Jim C. Macdonald

Nebraska Beef Cattle Reports

Summary with Implications

This finishing study evaluated the effect of feeding palm oil products on performance and carcass characteristics, and beef quality measures. Dietary treatments included no supplemental fat or feeding: crude palm oil, crude high-oleic palm oil (HOPO), refined, bleached, and de-odorized palm olein oil (RBD olein), or tallow. Supplemental fat was included at 3.5% of diet dry matter. Cattle fed diets containing supplemental fat had improved feed conversion compared to no supplemental fat. Feeding cattle crude palm oil resulted in improved F:G compared to cattle fed RBD olein, with steers fed tallow and crude HOPO being intermediate and …

Comparison Of Spayed Heifer Performance To Intact Heifers Fed Melengestrol Acetate On Feedlot Performance And Carcass Characteristics, Tate S. Johnson, Rebecca L. Mcdermott, Halden J. Clark, Jim C. Macdonald, Jessica L. Sperber

Nebraska Beef Cattle Reports

Summary with Implications

Pregnancy and estrous cycle management are critical considerations when feeding intact heifers in the feedyard. Alternatively, producers can opt to spay heifers, which eliminates hormonal fluctuations associated with the estrous cycle. A proportion of U.S. feedlots have spayed heifers on feed, although there is limited data comparing the performance of spayed heifers to intact heifers. A commercial feedlot experiment was conducted to compare performance between spayed and intact heifers fed melengestrol acetate. Intact heifers had greater ADG and tended to have greater DMI compared to spayed heifers, although no difference in feed conversion was observed. Carcass weight …

Summary Of The Value Of Distillers Grains Plus Solubles For Feedlot Cattle, Rebecca L. Mcdermott, Jim C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

Summary with Implications

An analysis of over 6,100 head of finishing cattle across 50 experiments was conducted to determine the feeding value of full-fat and de-oiled distillers grains. This analysis was limited to studies where dry-rolled corn, high-moisture corn, or a combination of the two corn processing methods were displaced by 15% or more distillers grains. Dry distillers grains plus solubles (DDGS), modified distillers grains plus solubles (MDGS), and wet distillers grains plus solubles (WDGS) were evaluated as either full-fat or de-oiled. The feeding value of distillers grains was assessed relative to a corn-based control diet containing no distillers. Results …

A Phylogenetic Analysis Of Avian-Associated Ixodes Scapularis & Implications For Lyme Disease, Nora Correiro

Honors Theses

Lyme disease, caused by the bacterium Borrelia burgdorferi and transmitted by the blacklegged tick Ixodes scapularis, is the most common vector-borne illness in the United States. Whereas Lyme disease is concentrated in the northeastern US, I. scapularis exists in the southeast as well, and the ticks in these regions generally separate into northern and southern evolutionary lineages. Although recent analyses suggest unidirectional and limited gene flow from north to south due to behavioral, ecological, and environmental barriers, the mechanisms underlying the persistence of this limited gene flow remain unclear. This study examines whether bird-mediated dispersal may facilitate south-to-north gene …

Association Of Serotonin And Ergot Alkaloids On Tissue Partitioning And Contractile Response Of Bovine Blood Vessels, Eriton E. L. Valente, David L. Harmon, John May, Huihua Ji, Ronald J. Trotta, James L. Klotz

Animal and Food Sciences Faculty Publications

Ergot alkaloids can bind serotonin (5-HT) receptors interfering with many physiological functions. However, the mechanism has not been completely established. The objective was to evaluate whether the association of 5-HT and the ergot alkaloid, ergovaline, in a 24-h pre-incubation can affect vascular tissue partitioning and contractile responses. Cross-sections of saphenous veins from five steers were used. In the tissue partitioning experiment, the treatments were the combination of three levels of ergovaline (2.01 × 10⁻⁸ M, 2.01 × 10⁻⁷ M and 2.01 × 10⁻⁶ M) with three levels of 5-HT and a control (5 × 10⁻⁸ M, …

Effects Of Perching Behavior In Broilers On Welfare And Production Benefits, Natalie G. Bunn

Honors Theses and Capstones

Broiler chickens are bred for feed efficiency, rapid growth rate, and muscle development. Conventional broiler farms utilize fast-growing breeds that reach market weight rapidly and present welfare concerns. Primarily raised indoors with little enrichment, the genetics and husbandry practices of broilers encourage inactivity and perpetuate health problems such as skeletal deformities, lameness, and pododermatitis. Despite the commonness of these issues, there is little research on the reduction of such health complications through production management. This study investigated methods to promote perching behavior and the effects of riser perch and platform perch utilization in broilers. A mixed flock of slow-growing Gray …

Electronic Bills Of Lading And Blockchain Technology: A Regulatory Perspective, Hsin-Hua Tsai

Journal of Marine Science and Technology–Taiwan

Information and communications technology (ICT) systems are increasingly relevant to the maritime sector. Regarding the revolution of the bill of lading, many shipping lines and other technology startups have been looking into developing viable electronic transport documents that have the same functions as paper bills of lading to reduce supply chain risks. The limitations of the paper-based process have become increasingly visible in the COVID-19 crisis. The main purpose of the article is to analyze the application of blockchain bills of lading, which utilizes computational logic to create the digital ledger, and users can set up algorithms and rules to …

Intelligent Formation Control Using Orfbls And Adaptive Backstepping Sliding-Mode Control To Address Uncertain Tilting In Multi-Quadrotors During Wind Gusts, Ching-Chih Tsai, Chun-Fu Mao, Kumail Hussain

Journal of Marine Science and Technology–Taiwan

In terms of aerial robotics, stable and precise formation control is a significant challenge for tilting multi-quadrotors during disturbances. This paper proposes a fixed-time formation control strategy for tilting multi-quadrotors to address the effect of external wind gusts. The observer accurately predicts environmental disturbances and an adaptive backstepping sliding-mode control (ABSMC) method with an output recurrent fuzzy broad learning system (ORFBLS) addresses these disturbances within a finite time. ORFBLS dynamically adjusts its structure through a growing ORFBLS structure to allow nodes to be added as needed. The method's stability is validated using Lyapunov stability theory and ensures the convergence of …

Visually Guided Landing System On Ship Deck For Multicopter, Dong-Lin Li, Shih-Kai Lee, Tzu-Hsiang Chou

Journal of Marine Science and Technology–Taiwan

Traditionally, high-value fish have been located at sea using helicopters. However, with advancements in technology, maritime drones have become increasingly important in recent years. Compared to traditional helicopter-based methods, drones offer significantly lower operational costs, making them cost-effective. However, the challenging sea conditions, including strong winds and the swaying motion of vessels, pose significant challenges for drone landings.

This paper proposes a stable approach for multicopter landing on ships at sea, incorporating improved marker detection, enhanced wind resistance control, and more accurate deck motion prediction. In the marker detection phase, we introduce a method to improve the accuracy of ArUco …

Ectomycorrhizal Fungi Associated With Pinus Species Of California's Northwestern Coast, Monika Audrey Richardson

Cal Poly Humboldt theses and projects

Ectomycorrhizal (ECM) fungi are vital for increasing host plant uptake of nutrients and stress tolerance within ECM forest ecosystems. The species assemblage of ECM fungi can vary locally in response to numerous biotic and abiotic environmental factors. In California’s northwestern coast the occurrence of three Pinus species, along with a disjunction between populations of Pinus muricata in Mendocino and Humboldt Counties, offer a unique opportunity to investigate the spatial and host effects on pine-associated ECM fungi. Ectomycorrhizal fungal community structure was quantified from host tree roots and sequenced sporocarps occurring at the same sites to create a project specific ITS …

Assessing A Half-Century Of Change In Understory Plant Range And Frequency In The Klamath Mountains Of California, Emily Jean Jackson

Cal Poly Humboldt theses and projects

The effects of climate change have and will continue to impact plant communities, including by potentially shifting species’ ranges towards topographically sheltered, cooler locations or higher elevations. In 2024, I resampled 140 vegetation plots established in 1969 in the Klamath Mountains of Northern California to assess range shifts of 46 understory species over a 55-year period. During this time, the elevational distribution of all species has increased significantly by an average of 26.9 m, but this increase was slower than necessary to track historic temperature niches given regional warming (215-311 m). Despite this overall increase, only four species exhibited significant …

Biological Use Of Molybdenum And Tungsten Stems Back To 3.4 Billion Years Ago, Rika Anderson, Aya S. Klos, Morgan S. Sobol, Joanne S. Boden, Eva E. Stüeken, Kurt O. Konhauser, Betül Kaçar

Biology Faculty Work

The biological significance of the transition metal molybdenum (Mo) lies in its function at the catalytic center of several enzymes that drive a wide spectrum of redox reactions underlying global biogeochemical cycles, yet a paradox persists. While modern life ubiquitously relies on Mo, geochemical evidence suggests that its availability in early Earth’s anoxic oceans was extremely limited. Modern organisms can use Mo down to trace levels; however, the rates of Mo-dependent metabolisms slow down when Mo availability decreases, posing fundamental questions about the extent to which changing Mo abundances shaped the evolution of molybdoenzymes, and when early life began harnessing …

Government Agencies In The United States Are Obstructing Native Species Restoration, Creating Regulatory Pits For Wildlife, Jeremy T. Bruskotter, L. Mark Elbroch, John Vucetich

Michigan Tech Publications

No abstract provided.

Restoring The Wild East: Predicting Public Support For Cougar And Red Wolf Reintroduction In The Eastern United States, Benjamin Ghasemi, Jeremy T. Bruskotter, John A. Vucetich, L. Mark Elbroch, Robyn S. Wilson, Neil H. Carter, Axel Moehrenschlager, Shelby Perry, Joseph W. Hinton

Michigan Tech Publications

Restoring large carnivores to human-dominated landscapes remains ecologically and socially contentious, though reintroduction may offer important benefits where coexistence is possible. Yet, efforts to reintroduce cougars (Puma concolor) and red wolves (Canis rufus) in the Eastern U.S. face major challenges, largely due to human-caused mortality and opposition. We employed the hazard acceptance model and structural equation modeling to investigate the socio-psychological drivers of public support for reintroduction. Survey data were collected from residents of the Eastern U.S. for cougars (n = 2665) and red wolves (n = 3825). For both species, support was positively associated with perceived personal and societal …

Recirculated Submarine Groundwater Discharge Dominates Nutrient Inputs And Enhances Eutrophication Risk In A Coastal Lagoon, Júlia Rodriguez-Puig, Clara Ruiz-González, Marc Diego-Feliu, Irene Alorda-Montiel, Aaron Alorda-Kleinglass, Daniel Romano-Gude, Andrea G. Bravo, Júlia Dordal-Soriano, Javier Gilabert, Sophia Bergeler, Celine Lavergne, Gemma Casas, Marisol Manzano, Jordi Garcia-Orellana, Valentí Rodellas

OES Faculty Publications

Submarine groundwater discharge (SGD) is a widely recognized pathway for nutrient transport to coastal systems. Prior studies overlook the unique biogeochemical signatures of different SGD pathways, neglecting their differences in biogeochemical transformations and spatiotemporal factors. Here, we present an integrated assessment of nutrient delivery through three SGD pathways (fresh SGD, long‐scale recirculated SGD, and short‐scale porewater exchange), alongside surface water inputs, to evaluate their seasonality and impact on nutrient dynamics in a coastal lagoon. We conducted five field surveys in March, July, and November 2021, July 2024, and March 2025 in the Mar Menor lagoon, an ecosystem facing severe ecological …

Severity And Duration-Dependent Aortic Stiffening In A Rabbit Coarctation Model Identifies Constitutive Material Parameters As Promising Regional Biomarkers For Hypertension Progression, Arash Ghorbannia, Jamasp Azarnoosh, John F. Ladisa Jr.

VMASC Publications

Coarctation of the aorta (CoA) alters hemodynamics and drives regional remodeling, yet the influence of severity and duration on constitutive properties is undefined. We quantified mechanical responses from CoA to identify constitutive parameters capturing stiffening across clinical severities and durations. Mild (≤ 12 mmHg), intermediate (13–20 mmHg), and severe (> 20 mmHg) CoA was created in rabbits for short, long, and prolonged durations (~ 1, 3, or 22 weeks, respectively). Stress–stretch curves from proximal and distal regions were fit with multiple constitutive models (Linear Elastic, Neo-Hookean, Yeoh, Ogden, Mooney–Rivlin, Holzapfel). Model performance was evaluated by normalized RMSE and R², with …