Life Sciences Commons^™

Caloric Restriction Prevents Obesity- And Intermittent Hypoxia-Induced Cardiac Remodeling In Leptin-Deficient Ob/Ob Mice, Aaron A. Jones, Sarah N. Framnes-Deboer, Arianne Shipp, Deanna M. Arble

Biological Sciences Faculty Research and Publications

Background:

Intermittent hypoxia (IH), a key characteristic of obstructive sleep apnea, is independently associated with cardiometabolic impairment. While endogenous leptin levels may provide cardioprotective effects against hypoxia, leptin resistance is common among obese individuals presenting with obstructive sleep apnea.

Methods:

Here, we assessed left ventricle (LV) function using M-mode echocardiography in lean wild-type, calorically-restricted ob/ob, and obese ob/ob mice before and after 6 days of IH to determine how obesity and intermittent hypoxia interact to affect cardiac function independent of leptin signaling.

Results:

Calorically-restricting ob/ob mice for 4 weeks prior to IH exposure prevented weight gain (−2.1 ± 1.4 …

Finding Biomarkers From Long-Non-Coding Rnas In Serum Of Patients With Non-Alcoholic Fatty Liver Disease, Nouran Yonis

Theses and Dissertations

Nonalcoholic fatty liver disease (NAFLD) is becoming a serious global public health problem as it is a highly prevalent condition, that begins as simple liver steatosis and develops into an inflammatory steatosis form known as non-alcoholic steatohepatitis (NASH). NAFLD has been linked with multiple comorbidities, which in certain cases predisposes to mortality secondary to extrahepatic neoplasms and coronary diseases. On the other hand, NASH leaves patients at risk of developing hepatocellular carcinoma (HCC). The incidence of NAFLD- based HCC is steadily increasing and is projected to surpass the incidence of HCC from viral origins. Long non-coding RNAs (lncRNAs) have been …

Effects Of Sensory Cues On Product Acceptability And Consumer Perceptions, Emotions, And Behavior, Cristhiam Gurdian Curran

LSU Doctoral Dissertations

Visual and cognitive cues can affect overall liking (OL) and consumer perceptions, emotions, and behavior. The first study explored the effect of product color difference on the liking, perception, and purchase intent (PI) of cheese-flavored-tortilla chips (CFTC) formulations (A and B) on serving plates (plastic, foam, and paper). Color differences between formulations influenced crunchiness and saltiness liking and perception, which together with overall flavor liking and formulation, mainly determined CFTC OL. Although having similar fracturability (N) and sodium content, formulation A had higher crunchiness and saltiness likings. PI was influenced by crunchiness, saltiness, and OF liking with 37, 49, and …

Modelling Aggressive Prostate Cancers Of Young Men In Immune-Competent Mice, Driven By Isogenic Trp53 Alterations And Pten Loss, Javier Octavio Mejía-Hernández, Simon P Keam, Reem Saleh, Fenella Muntz, Stephen B Fox, Arielle Kogan, Jennifer Huynh, Cassandra Litchfield, Franco Caramia, Guillermina Lozano, Hua He, James M You, Shahneen Sandhu, Scott G Williams, Ygal Haupt, Sue Haupt

Faculty, Staff and Student Publications

Understanding prostate cancer onset and progression in order to rationally treat this disease has been critically limited by a dire lack of relevant pre-clinical animal models. We have generated a set of genetically engineered mice that mimic human prostate cancer, initiated from the gland epithelia. We chose driver gene mutations that are specifically relevant to cancers of young men, where aggressive disease poses accentuated survival risks. An outstanding advantage of our models are their intact repertoires of immune cells. These mice provide invaluable insight into the importance of immune responses in prostate cancer and offer scope for studying treatments, including …

Turning Nonselective Inhibitors Of Type I Protein Arginine Methyltransferases Into Potent And Selective Inhibitors Of Protein Arginine Methyltransferase 4 Through A Deconstruction-Reconstruction And Fragment-Growing Approach, Giulia Iannelli, Ciro Milite, Nils Marechal, Vincent Cura, Luc Bonnefond, Alessandra Feoli, Yalong Wang, Alessandra Cipriano, Monica Viviano, Mark T Bedford, Jean Cavarelli, Sabrina Castellano, Gianluca Sbardella

Faculty, Staff and Student Publications

Protein arginine methyltransferases (PRMTs) are important therapeutic targets, playing a crucial role in the regulation of many cellular processes and being linked to many diseases. Yet, there is still much to be understood regarding their functions and the biological pathways in which they are involved, as well as on the structural requirements that could drive the development of selective modulators of PRMT activity. Here we report a deconstruction-reconstruction approach that, starting from a series of type I PRMT inhibitors previously identified by us, allowed for the identification of potent and selective inhibitors of PRMT4, which regardless of the low cell …

Characterization Of The Plastic Scintillator Detector System Exradin W2 In A High Dose Rate Flattening-Filter-Free Photon Beam, Sara Thrower, Surendra Prajapati, Shannon Holmes, Emil Schüler, Sam Beddar

Faculty, Staff and Student Publications

(1) Background: The Exradin W2 is a commercially available scintillator detector designed for reference and relative dosimetry in small fields. In this work, we investigated the performance of the W2 scintillator in a 10 MV flattening-filter-free photon beam and compared it to the performance of ion chambers designed for small field measurements. (2) Methods: We measured beam profiles and percent depth dose curves with each detector and investigated the linearity of each system based on dose per pulse (DPP) and pulse repetition frequency. (3) Results: We found excellent agreement between the W2 scintillator and the ion chambers for beam profiles …

Recovery Of Soil Microbial Diversity And Functions Along A Tropical Montane Forest Disturbance Gradient, Renee Sniegocki, Jessica B. Moon, Abigail L. Rutrough, Jude Gireneus, Jaya Seelan Sathiya Seelan, Michael C. Farmer, David C. Weindorf, Kusum Naithani

Biological Sciences Faculty Publications and Presentations

Logging and forest conversion are occurring at alarming rates in tropical forests. These disturbances alter soil microbial community structure and functions. While direct links between changes in soil properties, such as pH and microbial community structure are well established, the indirect effects of logging and forest conversion on soil microbial community structure and functions are poorly understood. We used a space-for-time substitution to investigate the changes in soil microbial diversity and functions across a forest recovery gradient in the tropical montane forests of northern Borneo. We used surface (top 5 cm) soil to assess soil physicochemical and microbial (next-generation DNA …

The Southern Flying Squirrel (Glaucomys Volans) In Lincoln, Nebraska, Melissa J. Panella, Shaun M. Dunn, Thomas E. Labedz, Hugh H. Genoways

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The southern flying squirrel (Glaucomys volans) is considered a species of greatest conservation need in Nebraska and listed as threatened in the state. Historically, the geographic range of the southern flying squirrel in Nebraska has been restricted to five eastern counties from a northern suburb of Omaha, Douglas Co., southward in the four counties of Sarpy, Otoe, Nemaha, and Richardson, all bordering the Missouri River on the east. In late November of 2018, a resident of Lincoln, Lancaster Co., Nebraska, contacted the Nebraska Game and Parks Commission about an animal found dead in his yard. This animal proved …

Microwave Coupled Hot-Air Drying Control Of Celery Stalks Based On Volatile Compounds Detection, Liu Xu, Song Chunfang

Food and Machinery

Objective:In order to preserve more volatile components of celery and reduce its moisture content. Methods:A hot-air coupled microwave drying system based on aroma detection was designed. An electronic nose (PEN3) was applied to detect volatile components, assisted with GC-MS to measure the specific content of volatile components. Based on constant temperature drying (50, 60, 70 and 80 ℃), sensor 7 and 9 were chosen for subsequent combined control. Results:Comparing with constant temperature drying, the combined control drying using volatile compounds detection could retain more volatile substances, and a good balance had been achieved between the quality of …

European Mushroom Assemblages Are Phylogenetically Structured By Temperature, Claus Bässler, Jacob Heilmann-Clausen, Carrie Andrew, Lynne Boddy, Ulf Büntgen, Jeffrey Diez, Et. Al.

Michigan Tech Publications, Part 1

Recent global warming affects species compositions at an unprecedented rate. To predict climate-induced changes in species assemblages, a better understanding of the link between species occurrence and climate is needed. Macrofungal fruit body assemblages are correlated with the thermal environment at the European scale. However, it is still unknown whether macrofungal communities are also phylogenetically structured by thermal environments. Thermal environments are characterized by annual temperature means but also by intra-annual temperature variability (hereafter termed temperature seasonality), which are both considered in this study. Here, we used distribution data of 2882 species based on fruit body records across Europe to …

Genome-Wide Association Analyses Of Physical Activity And Sedentary Behavior Provide Insights Into Underlying Mechanisms And Roles In Disease Prevention, Zhe Wang, Andrew Emmerich, Nicolas J. Pillon, Tim Moore, Daiane Hemerich, Marilyn C. Cornelis, Eugenia Mazzaferro, Siacia Broos, Tarunveer S. Ahluwalia, Traci M. Bartz, Amy R. Bentley, Lawrence F. Bielak, Mike Chong, Audrey Y. Chu, Diane Berry, Rajkumar Dorajoo, Nicole D. Dueker, Elisa Kasbohm, Bjarke Feenstra, Mary F. Feitosa, Mark A. Sarzynski Ph.D., Faha, Facsm, Et. Al.

Faculty Publications

Although physical activity and sedentary behavior are moderately heritable, little is known about the mechanisms that influence these traits. Combining data for up to 703,901 individuals from 51 studies in a multi-ancestry meta-analysis of genome-wide association studies yields 99 loci that associate with self-reported moderate-to-vigorous intensity physical activity during leisure time (MVPA), leisure screen time (LST) and/or sedentary behavior at work. Loci associated with LST are enriched for genes whose expression in skeletal muscle is altered by resistance training. A missense variant in ACTN3 makes the alpha-actinin-3 filaments more flexible, resulting in lower maximal force in isolated type IIA muscle …

Impact Of Virtual Vs. In-Person School On Children Meeting The 24-H Movement Guidelines During The Covid-19 Pandemic, Christopher D. Pfledderer, Michael Beets, Sarah Burkart Mph, Ph.D., Elizabeth Adams Potter, Robert Weaver Med, Phd, Xuanxuan Zhu, Bridget Armstrong

Faculty Publications

The pandemic mitigation strategy of closing schools, while necessary, may have unintentionally impacted children’s moderate-to-vigorous physical activity (MVPA), sleep, and time spent watching screens. In some locations, schools used hybrid attendance models, with some days during the week requiring in-person and others virtual attendance. This scenario offers an opportunity to evaluate the impact of attending in-person school on meeting the 24-h movement guidelines. Children (N = 690, 50% girls, K–5th) wore wrist-placed accelerometers for 14 days during October/November 2020. Parents completed daily reports on child time spent on screens and time spent on screens for school. The schools’ schedule was …

Co-Transcriptomic Analysis Of The Maize–Western Corn Rootworm Interaction, Lise Pingault, Saumik Basu, Neetha N. Vellichirammal, William Paul Williams, Gautam Sarath, Joe Louis

Department of Entomology: Faculty Publications

The Western corn rootworm (WCR; Diabrotica virgifera virgifera) is an economically important belowground pest of maize. Belowground feeding by WCR is damaging because it weakens the roots system, diminishes nutrient uptake, and creates entry points for fungal and bacterial pathogens and increases lodging, all of which can significantly suppress maize yields. Previously, it was demonstrated that belowground herbivory can trigger plant defense responses in the roots and the shoots, thereby impacting intraplant communication. Although several aspects of maize-WCR interactions have been reported, co-transcriptomic remodeling in the plant and insect are yet to be explored. We used a maize genotype, …

Australian Cleotychini Planthoppers: Review Of The Genus Cleotyche Emeljanov, 1997 With Three New Species (Hemiptera: Fulgoromorpha: Dictyopharidae), Jérôme Constant, Linda Semeraro, Melinda L. Moir

Biosecurity Research Articles

The genus Cleotyche in the monotypic Australian planthopper tribe Cleotychini (Fulgoroidea: Dictyopharidae) is reviewed. The subgenus Griseotyche subgen. nov. is described to accommodate one species Cleotyche blanda Emeljanov, 2011 while the second species, C. mariae Emeljanov, 1997, is retained in the subgenus Cleotyche (Cleotyche) Emeljanov, 1997. Three new species of Cleotyche (Cleotyche) from Queensland, C. (Cleotyche) christinae sp. nov. from Cania Gorge National Park, C. (Cleotyche) francescoi sp. nov. from Eurimbula National Park and C. (Cleotyche) montana sp. nov. from Blackdown Tableland National Park are described and compared to the …

Disagreements And Proper Respect In Farm/Ranch Succession, Allan Vyhnalek

Cornhusker Economics

Adapted from "Does Disrespect Have a Place in Your Ag Legacy?" Volume 6, Issue 1, February 2021, Ag Legacy, by Caleb Carter, consultant to the Department of Agricultural and Applied Economics in the University of Wyoming College of Agriculture and Natural Resources.

Briefly covers disagreements and proper respect in farm/ranch succession.

Smokejumper Obituary: Birky, Luke Amon (Missoula 1945), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Evaluating Success Factors And Challenges Among Small-Scale Agricultural Producers: A Texas Case Study, Katie Tritsch, Ken Mix, Michelle L. Edwards, Manuel Piña Jr.

Journal of Rural Social Sciences

Literature on small farms in the U.S. is limited though they are the most numerous farm type, generate over twenty percent of agricultural production, and are more likely to be operated by historically underserved (i.e., beginning, minority, veteran, women, young) farmers than large-scale farms. This article details an online survey study of small-scale agricultural producers using a purposive sample from Texas. We used cross tabulations to evaluate qualitative operational and demographic (e.g., age, gender) factors of success, finding several significant variables with moderate effect sizes. Generally, producers regarded quality of life as more important to success than profitability. Producers’ top …

Ungulate Herbivores As Drivers Of Aspen Recruitment And Understory Composition Throughout Arid Montane Landscapes, Elizabeth S. Reikowski, Tyler Refsland, J. Hall Cushman

Aspen Bibliography

Herbivory by wild and domestic ungulates can influence tree recruitment and understory forest communities throughout the world. Herbivore-driven declines in tree recruitment have been observed for quaking aspen (Populus tremuloides), a foundation species whose health and management is recognized as a critical priority throughout much of its range. Livestock fencing is commonly used to promote aspen regeneration, but its effectiveness is rarely assessed, especially across large spatial scales. Using a livestock-reduction experiment, we evaluated the effects of ungulate herbivory on aspen in the Great Basin and southern Cascades, an expansive and environmentally heterogeneous region where aspen faces the …

Intrinsic Disorder In Proteins Associated With Neurodegenerative Diseases, Akshatha Ganne

Theses and Dissertations

A protein’s structure is determined by its amino acid sequence and post-translational modifications, and provides the basis for its physiological functions. Roughly a third of the proteome in mammalian species comprises proteins that contain highly unstructured or intrinsically disordered regions. Proteins with extensive unstructured regions are referred to as intrinsically disordered proteins (IDPs). Recent advances in experimental and computational analyses predicted multiple interacting partners for the disordered regions of proteins, implying critical roles in signal transduction and regulation of biological processes. This dissertation has studied the connection between the misfolding of a protein and an insoluble protein using the ability …

Path Planning Of Food Robot Based On Density Peak Clustering Parallel Sparrow Search Algorithm, Hao Jie, Tang Ye-Jian

Food and Machinery

Objective:In order to improve the efficiency of multi-point moving path planning of food picking robot, a path planning method of food picking robot based on density peak clustering parallel sparrow search algorithm is proposed.Methods:The path planning model of food picking robot was established with the total moving distance, path smoothness between points and moving safety as the evaluation indexes. While ensuring the moving safety of the robot, the path smoothness was improved and the moving distance was reduced as much as possible. The density peak clustering sparrow search algorithm (DSSA) was designed, as the improved density peak …

A Comparison Of The Effectiveness Of Cocos Nucifera (Coconut) Water As A Rooting Hormone In A Hydroponic And Conventional Set-Up In Growing Ocimum Basilicum (Basil), Trisha Ysabel A. Reodica, Luis Raphael L. Reyes, Fernel Anne J. Cadiz, Jessica Paulyn A. Villa, Pio Jeremiah G. Tagra, Elizabeth Justinne I. Carag, Elisha Jaeriane A. Almeda, Jericho R. Castañeda, Hans Franco A. Amar, Jose Mari M. Calamlam, Gerald B. Gamboa

Sinaya: A Philippine Journal for Senior High School Teachers and Students

Food shortage is still a problem in the Philippines since millions of people may be classified as undernourished. Efforts to augment the insufficiency include developing an efficient planting method as a source of food. This experimental study tested the effectiveness of coconut water as a rooting hormone on hydroponically and conventionally grown basil plants by measuring the height, fresh weight, leaf length, and root length for three weeks, which also served as the dependent variables. Consequently, two (2) hydroponic set-ups and two (2) conventional set-ups utilize a randomized block design. The set-ups containing coconut water served as the independent variables, …

Frequency Of Bystander Exposure To Antibiotics For Enteropathogenic Bacteria Among Young Children In Low-Resource Settings, Elizabeth T. Rogawski Mcquade, Stephanie A. Brennhofer, Sarah E. Elwood, Timothy L. Mcmurry, Joseph A. Lewnard, Estomih R. Mduma, Sanjaya Shrestha, Najeeha Talat Iqbal, Pascal O. Bessong, Gagandeep Kang

Department of Paediatrics and Child Health

Children in low-resource settings carry enteric pathogens asymptomatically and are frequently treated with antibiotics, resulting in opportunities for pathogens to be exposed to antibiotics when not the target of treatment (i.e., bystander exposure). We quantified the frequency of bystander antibiotic exposures for enteric pathogens and estimated associations with resistance among children in eight low-resource settings. We analyzed 15,697 antibiotic courses from 1,715 children aged 0 to 2 y from the MAL-ED birth cohort. We calculated the incidence of bystander exposures and attributed exposures to respiratory and diarrheal illnesses. We associated bystander exposure with phenotypic susceptibility of E. coli isolates in …

Participatory And Spatial Analyses Of Environmental Justice Communities' Concerns About A Proposed Storm Surge And Flood Protection Seawall, Judith Taylor, Norman S. Levine, Ernest Muhammad, Dwayne E. Porter, Annette M. Watson, Paul A. Sandifer

Faculty Publications

In response to increasing threats from sea-level rise and storm surge, the City of Charleston, South Carolina, and the US Army Corps of Engineers (USACE) propose constructing a seawall around the Charleston peninsula. The proposed seawall will terminate close to lower wealth, predominantly minority communities. These communities are identified as environmental justice (EJ) communities due to their history of inequitable burdens of industrial and urban pollution and proximity to highways and US Environmental Protection Agency (EPA) designated Superfund sites. The present study documents community concerns and opinions related to the proposed seawall, existing flooding problems, and other issues. The project …

The Impact Of Self-Agitating Anaerobic Batch Digester Design On Biogas Production Of Cattle Manure Co-Digested With Lemna Minor, Aj S. Navarro, Jasmin Eloisa R. Cawaling, Stefan B. Bronoso, Hui L. Cao, Anton Marco G. Carlos, Andrea Dominique J. Garcia, Adrian D. Gendrano, Jessica Mae T. Lee, Ailyn A. Ojeda, Fritz M. Ferran, Christian Jeff G. Cariaga

Sinaya: A Philippine Journal for Senior High School Teachers and Students

The continuation of utilizing fossil fuels as cooking energy sources in rural communities in the Philippines causes more citizens to be at risk of developing numerous health illnesses. This study aimed to propose a potential solution to this problem by innovating a self-agitating anaerobic batch digester, promoting biogas production of cattle manure co-digested with Lemna minor. Two anaerobic batch digester designs, one with baffles and one without, were observed within 22 days to determine the impact of the anaerobic digester design on mixing and biogas production yield. The study contained two pairs of anaerobic batch digesters, the initial and improved …

Evaluation Of Bio Briquettes Made From Musa Acuminata Colla, Musa Acuminata And Musa Balbisiana Silk, And Citrus Reticulata And Citrus Sinensis Peels, Allegra Fatima R. Genuino, Janella Marie M. Tancuan, Quiara F. Ventura, Andrea Li S. Santos, Kariane Kyle R. Foo, Xavier Augusto Maria F. Samaco, Lucas Thaddeus A. Armada, Gavin Liam F. Lemoncito, Matthew Irwin B. Padrique, Gerard Deniel P. Almira, Antonio Rafael C. Torres, Jose Mari M. Calamlam, Gerald B. Gamboa

Sinaya: A Philippine Journal for Senior High School Teachers and Students

Accumulation of food waste and the burning of coal emit harmful chemicals which contribute to environmental problems such as climate change and global warming. These also risk the health of people, which causes deaths. Briquettes help improve and preserve the environment by lessening food waste and coal emissions. This study aims to determine the best treatment for briquettes to help disadvantaged communities and alleviate the adverse effects on the environment and health. A combination of banana (Musa acuminata Colla (AA Group) 'Lakatan' and Musa acuminata × M. balbisiana (AAB Group) 'Silk', and orange (Citrus × reticulata and Citrus × sinensis) …

Status Of Barking Owl Ninox Connivens In South-West Australia, Robert A. Davis, Leo Joseph, Ronald E. Johnstone

Research outputs 2022 to 2026

Barking Owl Ninox connivens has two recognised subspecies in Australia: N. c. connivens and N. c. peninsularis. N. c. connivens currently includes the isolated south-west Australian population, which is separated from eastern populations by the Nullarbor Plain and from N. c. peninsularis to the north. N. c. connivens in south-west Australia occurs from near Perth in the north, east to Northam and south-east to Katanning and Bremer Bay; it has been treated subspecifically as N. c. addendaMathews, 1912, but this name is not currently in use. Given concern over the apparent rarity of the south-west Australian population, we sought to …

Pim1 Promotes Hepatic Conversion By Suppressing Reprogramming-Induced Ferroptosis And Cell Cycle Arrest, Miaomiao Luo, Wankun Deng, Jiaqi Zhou, Lihui Liu, Lina Mao, Wenbo Peng, Jian Chen, Qiangsong Wang, Yilai Shu, Yu Xue, Pengyu Huang

Faculty, Staff and Student Publications

Protein kinase-mediated phosphorylation plays a critical role in many biological processes. However, the identification of key regulatory kinases is still a great challenge. Here, we develop a trans-omics-based method, central kinase inference, to predict potentially key kinases by integrating quantitative transcriptomic and phosphoproteomic data. Using known kinases associated with anti-cancer drug resistance, the accuracy of our method denoted by the area under the curve is 5.2% to 29.5% higher than Kinase-Substrate Enrichment Analysis. We further use this method to analyze trans-omic data in hepatocyte maturation and hepatic reprogramming of human dermal fibroblasts, uncovering 5 kinases as regulators in the two …

Clonal Hematopoiesis Is Associated With Increased Risk Of Severe Neurotoxicity In Axicabtagene Ciloleucel Therapy Of Large B-Cell Lymphoma, Neeraj Y Saini, David M Swoboda, Uri Greenbaum, Junsheng Ma, Romil D Patel, Kaberi Das, Paolo Strati, Ranjit Nair, Luis Fayad, Sairah Ahmed, Hun Ju Lee, Swaminathan P Iyer, Raphael Steiner, Nitin Jain, Loretta Nastoupil, Sanam Loghavi, Guilin Tang, Roland L Bassett, Preetesh Jain, Michael Wang, Jason R Westin, Michael R Green, David A Sallman, Eric Padron, Marco L Davila, Frederick L Locke, Richard E Champlin, Guillermo Garcia-Manero, Elizabeth J Shpall, Partow Kebriaei, Christopher R Flowers, Michael D Jain, Feng Wang, Andrew P Futreal, Nancy Gillis, Sattva S Neelapu, Koichi Takahashi

Faculty, Staff and Student Publications

To explore the role of clonal hematopoiesis (CH) in chimeric antigen receptor (CAR) T-cell therapy outcomes, we performed targeted deep sequencing on buffy coats collected during the 21 days before lymphodepleting chemotherapy from 114 large B-cell lymphoma patients treated with anti-CD19 CAR T cells. We detected CH in 42 (36.8%) pretreatment samples, most frequently in PPM1D (19/114) and TP53 (13/114) genes. Grade ≥3 immune effector cell-associated neurotoxicity syndrome (ICANS) incidence was higher in CH-positive patients than CH-negative patients (45.2% vs. 25.0%, P = 0.038). Higher toxicities with CH were primarily associated with DNMT3A, TET2, and ASXL1 genes (DTA mutations). Grade …

Genetic- And Diet-Induced Ω-3 Fatty Acid Enrichment Enhances Trpv4-Mediated Vasodilation In Mice, Rebeca Caires, Tessa A C Garrud, Luis O Romero, Carlos Fernández-Peña, Valeria Vásquez, Julio F Cordero-Morales

Faculty, Staff and Student Publications

TRPV4 channel activation in endothelial cells leads to vasodilation, while impairment of TRPV4 activity is implicated in vascular dysfunction. Strategies that increase TRPV4 activity could enhance vasodilation and ameliorate vascular disorders. Here, we show that supplementation with eicosapentaenoic acid (EPA), an ω-3 polyunsaturated fatty acid known to have beneficial cardiovascular effects, increases TRPV4 activity in human endothelial cells of various vascular beds. Mice carrying the C. elegans FAT-1 enzyme, which converts ω-6 to ω-3 polyunsaturated fatty acids, display higher EPA content and increased TRPV4-mediated vasodilation in mesenteric arteries. Likewise, mice fed an EPA-enriched diet exhibit enhanced and prolonged TRPV4-dependent vasodilation …

Ablating Lgr5-Expressing Prostatic Stromal Cells Activates The Erk-Mediated Mechanosensory Signaling And Disrupts Prostate Tissue Homeostasis, Xing Wei, Li Zhang, Yiqun Zhang, Cody Cooper, Chris Brewer, Yi-Ting Wang, Hunter B Wessells, Jianwen Que, Mark A Titus, Vincenzino Cirulli, Adam Glaser, Tao Liu, Nicholas P Reder, Chad J Creighton, Li Xin

Faculty, Staff and Student Publications

Functional implication of stromal heterogeneity in the prostate remains incompletely understood. Using lineage tracing and light-sheet imaging, we show that some fibroblast cells at the mouse proximal prostatic ducts and prostatic urethra highly express Lgr5. Genetic ablation of these anatomically restricted stromal cells, but not nonselective ablation of prostatic stromal cells, rapidly induces prostate epithelial turnover and dedifferentiation that are reversed following spontaneous restoration of the Lgr5