Life Sciences Commons^™

Research Progress On The Influence Of Black Tea Fermentation Conditions On The Tea Pigment Formation, Cui Hong-Chun, Zhang Jian-Yong, Zhao Yun, Huang Hai-Tao, Ao Cun, Li Hong-Li, Shi Da-Liang

Food and Machinery

The research progress of the influence of fermentation temperature, fermentation humidity, fermentation time and exogenous additives on the formation of black tea pigment were reviewed. The future research and development trend of black tea pigment formation was prospected.

Effects Of Soaking Temperature On Rice Regenerationcharacteristics And Edible Quality, Liu Wei, Zhou Xiao-Yan, Shaw En-Xuan, Zhao Zhao, Wang Qiu-Yu

Food and Machinery

Objective：To study the effects of different soakingtemperatures on the short-term return of rice in rice， and providea certain theoretical basis for controlling starch food.Methods：The japonica rice was soaked at(20， 35， 50， 65， 80℃) for15min， using low-field nuclear magnetic resonance， differentialscanning calorimetry，texture analyzer， colorimeter， etc.tostudy its cooking characteristics， thermal characteristics， textureand color difference to reflect its retro gradation characteristics.Results：When the soaking temperature was 50℃， the soakingtemperaturewaslowerthanthestarchgelatin izationtemperature.After short-term retro gradation， it was conduciveto the retention of moisture in the rice grains， with the besttaste， the highest brightness， and a low degree of retro gradation.Conclusion：Soaking …

Optimization Of Extraction Process Of Polyphenols From Olive Leaves And Its Effect On Oxidative Stability Of Camellia Seed Oil, Shang Fang-Yuan, Pan Li, Gu Ke-Ren, Li Xue-Qin

Food and Machinery

Objective: The application of olive leaf, a by-product of olive oil production, in food industry was studied. Methods: Taking the yield of olive leaf polyphenols as the evaluation index, on the basis of single factor experiment, the effects of water bath temperature, extraction time and solid-liquid ratio on the yield of polyphenols were analyzed by Box-Behnken response surface method, the effects of olive leaf polyphenols on the oxidative stability of camellia seed oil were studied with peroxide value and acid value as indexes. Results: The optimum extraction conditions of polyphenols from olive leaves by water bath were …

Poliovirus Immunity Among Children Aged 6-11 And 36-48 Months In 14 Polio High-Risk Provinces Of Afghanistan: A Health-Facility-Based Study, Sajid Bashir Soofi, Maureen Martinez, Noha H. Farag, William S. Hendley, Derek Ehrhardt, Imran Ahmed, Imtiaz Hussain, William Weldon, Ahmed M. Kassem

Department of Paediatrics and Child Health

Afghanistan is one of two countries where wild poliovirus (WPV) type 1 remains endemic. We conducted a facility-based cross-sectional survey of antipoliovirus antibodies in children in 14 provinces of Afghanistan. The provinces were selected based on programmatic priorities for polio eradication. Children aged 6-11 and 36-48 months attending outpatient clinics were enrolled in the study. We collected venous blood, isolated serum, and conducted neutralization assays to detect poliovirus neutralizing antibodies. A total of 2086 children from the 14 provinces were enrolled. Among the enrolled children, 44.3% were girls; the median age in the 6-11-month group was 9.4 months, and in …

Proteômica Plasmática Quantitativa De Pacientes Sobreviventes E Não Sobreviventes De Covid-19 Internados No Hospital Revela Potenciais Biomarcadores Prognósticos E Alvos Terapêuticos, D C. Flora, A D. Valle, H Abs Pereira, T F. Garbieri, L. T. Grizzo, T J. Dionisio, A L. Leite, D Mc Rosa, C F. Santos, M Ar Buzalaf

Nebraska Center for Virology: Faculty Publications

O desenvolvimento de novas abordagens que permitam a avaliação precoce de quais casos de COVID-19 provavelmente se tornarão críticos e a descoberta de novos alvos terapêuticos são importantes. Neste estudo de coorte, foi avaliado o perfil proteômico e laboratorial do plasma de 163 pacientes internados no Hospital Estadual de Bauru (Bauru, SP, Brasil) entre 4 de maio e 4 de julho de 2020, que foram diagnosticados com COVID-19 por RT-PCR a partir de amostras de swab nasofaríngeo amostras. Amostras de plasma foram coletadas na admissão para análises laboratoriais de rotina e análise proteôomica quantitativa shotgun livre de marcadores. De acordo …

Salt Tolerance Qtls Of An Endemic Rice Landrace, Horkuch At Seedling And Reproductive Stages, Taslima Haque, Sabrina M. Elias, Samsad Razzaque, Sudip Biswas, Sumaiya Farah Khan, G. M. Nurnabi Azad Jewel, Md. Sazzadur Rahman, Thomas E. Juenger, Zeba I. Seraj

Department of Agronomy and Horticulture: Faculty Publications

Salinity has a significant negative impact on production of rice. To cope with the increased soil salinity due to climate change, we need to develop salt tolerant rice varieties that can maintain their high yield. Rice landraces indigenous to coastal Bangladesh can be a great resource to study the genetic basis of salt adaptation. In this study, we implemented a QTL analysis framework with a reciprocal mapping population developed from a salt tolerant landrace Horkuch and a high yielding rice variety IR29. Our aim was to detect genetic loci that contributes to the salt adaptive responses of the two …

Soil Nematode Trophic Group Composition And Influence On Growth Of Amaranthus Palmeri And Parthenium Hysterophorus, Orlando Garcia, Dana Garibaldi, Krissa Jhaveri, Jake Lanoue, Pushpa G. Soti

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Weeds have a significant impact on agricultural systems. They not only cause a loss in crop yield by competing with them for resources, but they can also serve hosts for several pests and parasties such as plant parasitic nematodes casusing additional crop loss. The aim of this study was to analyze plant-nematode feedback in two major weeds, Amaranthus palmeri S Watson and Parthenium hysterophorus L. First, a field survey was conducted to determine the rhizosphere nematode trophic groups associated with these two plants in the summer of 2020 and 2021. Then a 6-week greenhouse study was conducted where the two …

Immediate And Residual-Effects Of Sole And Intercropped Grain Legumes In Maize Production Systems Under Rain-Fed Conditions Of Northern Tanzania, E. Mugi-Ngenga, L. Bastiaans, N. P.R. Anten, S. Zingore, K. E. Giller

All Peer-Reviewed Publications

Intercropping of maize with grain-legume crops predominates on smallholder farms in East Africa. However, the growth and development of crops and their interaction with fertilizer in intercropping systems are not well understood. We sought to answer the questions: (i) what are the effects of fertilizer on the growth and development of maize-pigeonpea and maize-lablab intercropping systems under variable agroecological conditions? (ii) what are the residual effects of two seasons of sole and intercrops of maize, pigeonpea and lablab on a succeeding maize crop? We studied pure stands of maize, long-duration pigeonpea, medium-duration pigeonpea and lablab, and additive intercropping combinations on …

Forest Cover And Geographic Distance Influence Fine-Scale Genetic Structure Of Leaf-Toed Geckos In The Tropical Dry Forests Of Western Mexico, Connor M. French, Casey-Tyler Berezin, Isaac Overcast, Fausto R. Méndez De La Cruz, Saptarsi Basu, Roberto Lhemish Martínez Bernal, Robert W. Murphy, Michael J. Hickerson, Christopher Blair

Publications and Research

The biodiversity within tropical dry forests (TDFs) is astounding and yet poorly catalogued due to inadequate sampling and the presence of cryptic species. In the Mexican TDF, endemic species are common, and the landscape has been continually altered by geological and anthropogenic changes. To understand how landscape and environmental variables have shaped the population structure of endemic species, we studied the recently described species of leaf-toed gecko, Phyllodactylus benedettii, in coastal western Mexico. Using double-digest restriction site-associated DNA sequencing data, we first explore population structure and estimate the number of ancestral populations. The results indicate a high degree of …

Physical Activity Is Related To Mental Health And Sexual Orientation Among Women In College, Ellen M. Brooks, Oliver W.A. Wilson, Lucas Elliott, Michele Duffey, Melissa Bopp

International Journal of Exercise Science

International Journal of Exercise Science 15(5): 1347-1356, 2022. Non-heterosexual women tend to report lower physical activity and poorer mental health than their heterosexual counterparts. The purpose of this study was to examine differences in mental health (stress and depression) and physical activity among female college students by sexual orientation. Students self-reported socio-demographic characteristics, physical activity, perceived stress, and depressive symptoms via an online survey. Correlations, independent samples t-tests, and multiple regression analyses were used to examine relationships between perceived stress, depressive symptoms, physical activity, and sexual orientation. Most participants (n = 1072, 20.0 ± 1.5 years) identified as heterosexual …

Impact Of Two Types Of Fitness Programs On Soldier Physical Fitness, Aaron G. Parks, William H. Murrah, Wendi H. Weimar, Paige A. Mchenry, Donald Bigham, Kkevin Giordano, Joellen M. Sefton

International Journal of Exercise Science

International Journal of Exercise Science 15(4): 1326-1346, 2022. This study compared an expert supervised, fully resourced physical training (PT) program compared to a traditional physical training PT plan on Army Officer Candidate School (OSC) soldier fitness outcomes. This retrospective cohort study compared 228 OCS soldiers (179 male [26.74±3.78 years] and 49 female [26.55±4.18 years]) in two companies for 12 weeks. One company participated in a fully resourced PT program designed by fitness experts to improve overall fitness and mobility (TAP-C). One company participated in traditional physical training designed to excel on the Army combat fitness test (ACFT, includes deadlift, power …

Three-Dimensional Motion Analysis For Occupational Therapy Upper Extremity Assessment And Rehabilitation: A Scoping Review, Stephen W. Hill, Samantha Mong, Quyen Vo

The Open Journal of Occupational Therapy

Background: Three-dimensional (3D) human motion analysis provides objective, quantitative, and reliable kinematic data that are valuable in rehabilitation. Clinicians, including occupational therapists and other specialists, can apply this technology to quantify patients’ upper extremity (UE) motion during functional tasks. A better comprehension of altered body mechanics serves to guide clinical reasoning, develop evidence-based interventions, and monitor patients’ progress through follow-up. However, the scientific literature has yet to emphasize the practicality of using 3D motion analysis as a clinical measurement tool.

Method: This scoping review appraised 20 articles that used 3D motion analysis to quantify UE movements for individuals …

A New Species Of Chrysina Kirby (Coleoptera: Scarabaeidae: Rutelinae) From The Sierra Madre Occidental Of Mexico, With Notes On The Type Locality Of Chrysina Adelaida (Hope, 1841), David C. Robacker, David C. Hawks, Naidely Castillo, Brianna Miranda

Insecta Mundi

This paper presents a taxonomic review of the genus Boreocanthon Halffter, restored generic sta­tus, a group of ball-rolling (telocoprid) dung beetles (Coleoptera: Scarabaeidae: Scarabaeinae) endemic to North America. The genus, heretofore treated as a subgenus of Canthon, comprises 13 species, each keyed, diagnosed, illustrated and presented with information on distribution, relationships, and biology (*signifies restored generic combination): *Boreocanthon ateuchiceps (Bates, 1887); B. coahuilensis (Howden, 1966); *B. depressipennis (LeConte, 1859c); *B. ebenus (Say, 1823); *B. forreri (Bates, 1887); *B. integricollis (Schaef­fer, 1915); *B. lecontei (Harold, 1868); …

Acmaeodera Moesta Dugès, 1891 (Coleoptera: Buprestidae), A Poorly Known Species From Mexico, Richard L. Westcott, Jesus Romero-Napoles

Insecta Mundi

Two new state records are reported for the Mexican Acmaeodera moesta Dugès (Coleoptera: Bu­prestidae), a lectotype is designated for it, and clarifying discussion is provided.

According to Westcott (2020) there are 152 described valid species of Acmaeodera Eschscholtz in Mexico. Acmaeodera moesta Dugès (Fig. 1–3) was described from the state of Guanajuato, and it has not since been men­tioned in the literature except for listings in catalogs and checklists. Herein we record it from the states of México (MEX) and San Luis Potosí (SLP), designate a lectotype, and provide a detailed discussion.

Population Trajectory And Stressors Of Acropora Palmata Sites In The Florida Keys, Karen L. Neely, Kevin A. Macaulay, Kate S. Lunz

Marine & Environmental Sciences Faculty Articles

The decline of elkhorn coral, Acropora palmata, has been ongoing for decades, but the causes of decline and the resulting population status continue to be topics of study. Past efforts to categorize stressors have ranged from spatially and/or temporally focused efforts that detect local stressors but may miss broader patterns to meta-analyses that identify large-scale trends but may not account for finer-scale variability. We here conduct an analysis of sites surveyed across five years (2010-2015) and much of the Florida Reef Tract in order to look at large-scale patterns while also accounting for site, habitat, seasonal, and annual variability. …

Bee‑Safe Peptidomimetic Acaricides Achieved By Comparative Genomics, Vikas Jindal, Daqi Li, Leslie Catherine Rault, Soheila Fatehi, Rupinder Singh, Moritz Mating, Ye Zou, Ho‑Leung Ng, Krzysztof Kaczmarek, Janusz Zabrocki, Shunhua Gui, Guy Smagghe, Troy D. Anderson, Ronald J. Nachman, Yoonseong Park

Department of Entomology: Faculty Publications

The devastating Varroa mite (Varroa destructor Anderson and Trueman) is an obligatory ectoparasite of the honey bee, contributing to significant colony losses in North America and throughout the world. The limited number of conventional acaricides to reduce Varroa mites and prevent disease in honey bee colonies is challenged with wide-spread resistance and low target-site selectivity. Here, we propose a biorational approach using comparative genomics for the development of honey beesafe and selective acaricides targeting the Varroa mite-specific neuropeptidergic system regulated by proctolin, which is lacking in the honey bee. Proctolin is a highly conserved pentapeptide RYLPT (Arg-Tyr-Leu-Pro-Thr) known to …

Fresh Air For The Mire-Breathing Hypothesis: Sphagnum Moss And Peat Structure Regulate The Response Of Co2 Exchange To Altered Hydrology In A Northern Peatland Ecosystem, Ally O’Neill, Colin Tucker, Evan S. Kane

Michigan Tech Publications, Part 1

Sphagnum-dominated peatlands store more carbon than all of Earth’s forests, playing a large role in the balance of carbon dioxide. However, these carbon sinks face an uncertain future as the changing climate is likely to cause water stress, potentially reducing Sphagnum productivity and transitioning peatlands to carbon sources. A mesocosm experiment was performed on thirty-two peat cores collected from two peatland landforms: elevated mounds (hummocks) and lower, flat areas of the peatland (hollows). Both rainfall treatments and water tables were manipulated, and CO2 fluxes were measured. Other studies have observed peat subsiding and tracking the water table downward when experiencing …

Taxonomic Review Of The North American Dung Beetle Genus Boreocanthon Halffter, 1958 (Coleoptera: Scarabaeidae: Scarabaeinae: Deltochilini), W. D. Edmonds

Insecta Mundi

This paper presents a taxonomic review of the genus Boreocanthon Halffter, restored generic sta­tus, a group of ball-rolling (telocoprid) dung beetles (Coleoptera: Scarabaeidae: Scarabaeinae) endemic to North America. The genus, heretofore treated as a subgenus of Canthon, comprises 13 species, each keyed, diagnosed, illustrated and presented with information on distribution, relationships, and biology (*signifies restored generic combination): *Boreocanthon ateuchiceps (Bates, 1887); B. coahuilensis (Howden, 1966); *B. depressipennis (LeConte, 1859c); *B. ebenus (Say, 1823); *B. forreri (Bates, 1887); *B. integricollis (Schaef­fer, 1915); *B. lecontei (Harold, 1868); …

Lasioglossum (Dialictus) Semicaeruleum (Cockerell, 1895) (Hymenoptera: Halictidae) In Maryland: A Disjunct Population In Eastern North America?, Eugene J. Scarpulla

Insecta Mundi

Since 2004, three specimens of Lasioglossum (Dialictus) semicaeruleum (Cockerell, 1895) (Hyme­noptera: Halictidae) have been collected in Maryland. Other than three specimens from Wisconsin, there are no additional records of this western United States species known east of the Mississippi River. I document the three Maryland records and offer possible scenarios of how the specimens could have arrived in Mary­land.

Lasioglossum (Dialictus) semicaeruleum (Cockerell, 1895) (Hymenoptera: Halictidae) is an abundant western North American species that ranges from the Canadian Prairie Provinces (Alberta, Manitoba, and Saskatchewan) through the United States west of the Mississippi River (Arizona, California, Colorado, …

Genomics Reveals A New Genus And Species From A Single Female Specimen (Lepidoptera: Hesperiidae: Hesperiinae: Hesperiini: Moncina), Ernst Brockmann, Jing Zhang, Qian Cong, Nick V. Grishin

Insecta Mundi

New taxa in Hesperiidae (Lepidoptera: Papilionoidea) are traditionally proposed after inspection of male genitalia, which largely form the basis for Hesperiidae taxonomy. However, with genomic DNA se­quencing, even a single female specimen can be placed in a phylogenetic context of existing classification and taxonomically assigned with confidence. Genomic sequencing of an unusually patterned Hesperiidae female from San Martin, Peru, characterized by pearly spots outlining an inverted heart pattern on the rust-colored ventral hindwing, reveals that it represents an undescribed genus and species named here as Gemmia buechei Brockmann and Grishin, new genus and new species.

Neotropical regions are rich …

Machine Learning On Syngeneic Mouse Tumor Profiles To Model Clinical Immunotherapy Response, Zexian Zeng, Shengqing Stan Gu, Cheryl J Wong, Lin Yang, Nofal Ouardaoui, Wubing Zhang, X Shirley Liu

Faculty, Staff and Student Publications

Most patients with cancer are refractory to immune checkpoint blockade (ICB) therapy, and proper patient stratification remains an open question. Primary patient data suffer from high heterogeneity, low accessibility, and lack of proper controls. In contrast, syngeneic mouse tumor models enable controlled experiments with ICB treatments. Using transcriptomic and experimental variables from >700 ICB-treated/control syngeneic mouse tumors, we developed a machine learning framework to model tumor immunity and identify factors influencing ICB response. Projected on human immunotherapy trial data, we found that the model can predict clinical ICB response. We further applied the model to predicting ICB-responsive/resistant cancer types in …

Combined Trip13 And Aurora Kinase Inhibition Induces Apoptosis In Human Papillomavirus-Driven Cancers, Soma Ghosh, Tuhina Mazumdar, Wei Xu, Reid T Powell, Clifford Stephan, Pooja A Shah, Jeffery N Myers, Jing Wang, Mitchell J Frederick, Faye M Johnson

Faculty, Staff and Student Publications

PURPOSE: Human papillomavirus (HPV) causes >5% of cancers, but no therapies uniquely target HPV-driven cancers.

EXPERIMENTAL DESIGN: We tested the cytotoxic effect of 864 drugs in 16 HPV-positive and 17 HPV-negative human squamous cancer cell lines. We confirmed apoptosis in vitro and in vivo using patient-derived xenografts. Mitotic pathway components were manipulated with drugs, knockdown, and overexpression.

RESULTS: Aurora kinase inhibitors were more effective in vitro and in vivo in HPV-positive than in HPV-negative models. We hypothesized that the mechanism of sensitivity involves retinoblastoma (Rb) expression because the viral oncoprotein E7 leads to Rb protein degradation, and basal Rb protein …

Full Issue, Winthrop Mcnair Research Bulletin

The Winthrop McNair Research Bulletin

Winthrop McNair Research Bulletin Volume 5, Full Issue

The Relationship Between Nature Connectedness And Physical Activity Patterns In A Sample Of Collegiate Students, Faculty, And Staff, Destinee Waddy

The Winthrop McNair Research Bulletin

PURPOSE: A strong relationship exists between physical activity (PA) and nature connectedness (NC); the most physically active individuals may also be the most nature connected. Designing PA programs and modifying college campuses through the lens of biophilia can provide a more logical, evidence-based approach to improve overall health and wellness. The purpose of this study was to identify the relationship between physical activity and nature connectedness in a sample of collegiate faculty and students. METHODS: Participants completed two previously validated surveys: The International Physical Activity Questionnaire and the Connectedness to Nature Survey. Demographic data (age, race, campus affiliation) were also …

Locating Mutagen-Sensitivity Gene Mus109 In The Drosophila Melanogaster Genome Using Deficiency Mapping, Chandani Mitchell

The Winthrop McNair Research Bulletin

The complex processes involved in repairing damaged DNA are still being elucidated. Some genes that are known to have roles in the DNA repair process have been identified, such as the mutagen-sensitivity genes, or mus genes, in Drosophila melanogaster. However, the precise genomic location of some mus genes is still unknown, including mus109. It is known that mutations in mus109 cause chromosomal aberrations resulting in larval death, and previous research has mapped mus109 to a region of the X chromosome consisting of over 520,000 nucleotides and 41 genes. Therefore, this study aimed to locate mus109 using deficiency mapping. The mus109D2 …

Comparison Of The Effect Of Aerobic And Resistance Exercise On Short Term Memory On Healthy Adults, Frederick P. Wijaya, Ery Hermawati M.Sc, Willy Handoko M.Biomed

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

Background: Exercise is known to improve the cognitive function, including short-term memory. Exercise can induce structural and functional changes of the brain, i.e. synaptic plasticity. Exercise is categorized into two groups: aerobic exercise and resistance exercise. Studies have shown that both groups of exercises can improve short-term memory function.

Objectives: this research is aimed to compare the effect of aerobic and resistance exercise on short-term memory on healthy adults.

Methods: Participants were given the choice to be admitted into either exercise group, aerobic or resistance. Short-term memory (forward digit span) were measured before and after the intervention.

Results: there were …

Effects Of Ohio Opioid Prescribing Policy On Postsurgical Prescriptions Following Sports Procedures, Joseph Henningsen Md, Scott Huff Md, Andrew Schneider Md, Fady Hijji Md, Andrew Froehle Phd, Anil Krishnamurthy Md

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

Background: Prescribed opioid medication after orthopedic sports surgery has been shown to exceed patient requirements. In 2017, as a response to the opioid epidemic, Ohio passed Opioid Prescribing Guidelines (OPG) limiting narcotic prescriptions for acute pain. This study sought to evaluate the effects of the OPG on prescribing behavior of orthopedists following knee arthroscopy (KA), shoulder arthroscopy (SA), and anterior cruciate ligament reconstruction (ACLR). Methods: An institutional database was queried to calculate morphine equivalent dose (MED) prescribed at discharge, acute follow-up (<90 days), and chronic follow-up (>90 days) and compare MED pre- and post-OPG. Cases were identified over a 2-year period starting …

Establishing Safe Thresholds To Improve Exercise Capacity In Collegiate Athletes With Inflammatory Bowel Disease (Ibd): A Critically Appraised Topic, Emily C. Roberts, Jenny Toonstra, Andrea Cripps

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

Clinical Scenario: Inflammatory Bowel Disease (IBD) can present several challenges to athletic participation due to unpredictable disease activity and uncontrollable systemic symptoms that severely impact daily activities, and limit exercise/sports participation. Limited studies and a lack of standardized guidelines for physical activity (PA) and exercise are additional barriers for patients. Limited sources have determined that exercise interventions of low-to-moderate intensity are safe and feasible for IBD patients. In theory, such interventions could promote improvements in exercise capacity and overall well-being. Focused Clinical Question: Is there evidence to suggest that established safe exercise-intensities promote improvements in exercise capacity in collegiate athletes …

Mendelian Gene Identification Through Mouse Embryo Viability Screening., Pilar Cacheiro, Carl Henrik Westerberg, Jesse Mager, Mary E Dickinson, Lauryl M J Nutter, Violeta Muñoz-Fuentes, Chih-Wei Hsu, Ignatia B Van Den Veyver, Ann M Flenniken, Colin Mckerlie, Stephen A Murray, Lydia Teboul, Jason D Heaney, K C Kent Lloyd, Louise Lanoue, Robert E Braun, Jacqueline K White, Amie K Creighton, Valerie Laurin, Ruolin Guo, Dawei Qu, Sara Wells, James Cleak, Rosie Bunton-Stasyshyn, Michelle Stewart, Jackie Harrisson, Jeremy Mason, Hamed Haseli Mashhadi, Helen Parkinson, Ann-Marie Mallon, International Mouse Phenotyping Consortium, Genomics England Research Consortium, Damian Smedley

Faculty Research 2022

BACKGROUND: The diagnostic rate of Mendelian disorders in sequencing studies continues to increase, along with the pace of novel disease gene discovery. However, variant interpretation in novel genes not currently associated with disease is particularly challenging and strategies combining gene functional evidence with approaches that evaluate the phenotypic similarities between patients and model organisms have proven successful. A full spectrum of intolerance to loss-of-function variation has been previously described, providing evidence that gene essentiality should not be considered as a simple and fixed binary property.

METHODS: Here we further dissected this spectrum by assessing the embryonic stage at which homozygous …

The Multiform Genus Psyrassa Pascoe (Coleoptera: Cerambycidae: Elaphidiini): New Species, New Records, Synonyms And Transfers, Kimberly García, Antonio Santos-Silva

Insecta Mundi

This paper presents a taxonomic review of the genus Boreocanthon Halffter, restored generic sta­tus, a group of ball-rolling (telocoprid) dung beetles (Coleoptera: Scarabaeidae: Scarabaeinae) endemic to North America. The genus, heretofore treated as a subgenus of Canthon, comprises 13 species, each keyed, diagnosed, illustrated and presented with information on distribution, relationships, and biology (*signifies restored generic combination): *Boreocanthon ateuchiceps (Bates, 1887); B. coahuilensis (Howden, 1966); *B. depressipennis (LeConte, 1859c); *B. ebenus (Say, 1823); *B. forreri (Bates, 1887); *B. integricollis (Schaef­fer, 1915); *B. lecontei (Harold, 1868); …