Life Sciences Commons^™

Changes In Streptococcus Pneumoniae Susceptibility In Wisconsin: Implications For Clinical Treatment Decisions For Respiratory Infections, Erik Munson, Stephen C. Lavey, Megan R. Lasure, Barry C. Fox

Clinical Lab Sciences Faculty Research and Publications

Objective: In 2019, the American Thoracic Society and Infectious Diseases Society of America updated clinical practice guidelines for community-acquired pneumonia (CAP). In contrast to guidelines published in 2007, macrolide monotherapy for outpatients was made a conditional recommendation based on resistance levels. Local knowledge of current antimicrobial susceptibility is needed to guide management of CAP and other bacterial respiratory pathogens. The purpose of this study was to investigate antimicrobial susceptibility profiles and trending for Wisconsin Streptococcus pneumoniae isolates.

Design: Multi-center laboratory surveillance, with testing at a central location utilizing standardized susceptibility testing protocols.

Methods: Data published by the Wisconsin Department of …

A Novel Synthetic Phenotype Association Study Approach Reveals The Landscape Of Association For Genomic Variants And Phenotypes, Mária Škrabišová, Nicholas Dietz, Shuai Zeng, Yen On Chan, Juexin Wang, Jana Biová, Trupti Joshi, Kristin D. Bilyeu

Biomedical Sciences

Introduction: Genome-Wide Association Studies (GWAS) identify tagging variants in the genome that are statistically associated with the phenotype because of their linkage disequilibrium (LD) relationship with the causative mutation (CM). When both low-density genotyped accession panels with phenotypes and resequenced data accession panels are available, tagging variants can assist with post-GWAS challenges in CM discovery.

Objectives: Our objective was to identify additional GWAS evaluation criteria to assess correspondence between genomic variants and phenotypes, as well as enable deeper analysis of the localized landscape of association.

Methods: We used genomic variant positions as Synthetic phenotypes in GWAS that we named “Synthetic …

Anatomical Analysis Of Olfactory Sensory Neuron Regeneration Via Glomerular Synaptic Activity Markers In Adult Mice, William Wamack

Undergraduate Honors Theses

The olfactory system is a great model for studying regeneration due to the olfactory epithelium’s regenerative capability which makes it a potential a source of neural stem cells. The olfactory epithelium presents three types of cells: sustentacular cells which provide support and act as glial supporting cells; olfactory sensory neurons that are in charge of detecting odorant molecules in the air; and the stem cells that generated the aforementioned cell types. Olfactory sensory neurons are constantly dying and being replaced by new neurons originating from the stem cells that lie at the base of the olfactory epithelium. We have used …

Plant Productivity And Diversity In Coastal Wetlands In Mississippi – Impact Of Hydrological Extremes, Kodi Feldpausch

Master's Theses

Wetlands are highly productive ecosystems that provide important ecosystem services and are threatened by extreme hydrological events. I evaluated how diversion of freshwater like Bonnet Carré Spillway openings in Louisiana, US, affected vegetation productivity and diversity in salt marshes on the neighboring Mississippi Gulf Coast, US. My study area is two estuarine systems that differ in their proximity to the Bonnet Carré Spillway, elevation, and salinity including Hancock County Marshes on the west, and Grand Bay National Estuarine Research Reserve (NERR) on the east in Mississippi. I collected above- and belowground biomass samples in 2020 and 2021, developed linear mixed-effects …

Dietary And Complementary Feeding Practices Of Us Infants, 6 To 12 Months: A Narrative Review Of The Federal Nutrition Monitoring Data, Regan L Bailey, Jaime S Stang, Teresa A Davis, Timothy S Naimi, Barbara O Schneeman, Kathryn G Dewey, Sharon M Donovan, Rachel Novotny, Ronald E Kleinman, Elsie M Taveras, Lydia Bazzano, Linda G Snetselaar, Janet De Jesus, Kellie O Casavale, Eve E Stoody, Joseph D Goldman, Alanna J Moshfegh, Donna G Rhodes, Kirsten A Herrick, Kristin Koegel, Cria G Perrine, Tusarebecca Pannucci

Children’s Nutrition Research Center Staff Publications

Complementary foods and beverages (CFBs) are key components of an infant’s diet in the second 6 months of life. This article summarizes nutrition and feeding practices examined by the 2020 Dietary Guidelines Advisory Committees during the CFB life stage. Breastfeeding initiation is high (84%), but exclusive breastfeeding at 6 months (26%) is below the Healthy People 2030 goal (42%). Most infants (51%) are introduced to CFBs sometime before 6 months. The primary mode of feeding (ie, human milk fed [HMF]; infant formula or mixed formula and human milk fed [FMF]) at the initiation of CFBs is associated with the timing …

International Pooled Patient-Level Meta-Analysis Of Ketamine Infusion For Depression: In Search Of Clinical Moderators, Rebecca B Price, Nicholas Kissel, Andrew Baumeister, Rebecca Rohac, Mary L Woody, Elizabeth D Ballard, Carlos A Zarate, William Deakin, Chadi G Abdallah, Adriana Feder, Dennis S Charney, Michael F Grunebaum, J John Mann, Sanjay J Mathew, Bronagh Gallagher, Declan M Mcloughlin, James W Murrough, Suresh Muthukumaraswamy, Rebecca Mcmillan, Rachael Sumner, George Papakostas, Maurizio Fava, Rebecca Hock, Jennifer L Phillips, Pierre Blier, Paulo Shiroma, Peter Šóš, Tung-Ping Su, Mu-Hong Chen, Mikael Tiger, Johan Lundberg, Samuel T Wilkinson, Meredith L Wallace

Children’s Nutrition Research Center Staff Publications

Depression is disabling and highly prevalent. Intravenous (IV) ketamine displays rapid-onset antidepressant properties, but little is known regarding which patients are most likely to benefit, limiting personalized prescriptions. We identified randomized controlled trials of IV ketamine that recruited individuals with a relevant psychiatric diagnosis (e.g., unipolar or bipolar depression; post-traumatic stress disorder), included one or more control arms, did not provide any other study-administered treatment in conjunction with ketamine (although clinically prescribed concurrent treatments were allowable), and assessed outcome using either the Montgomery-Åsberg Depression Rating Scale or the Hamilton Rating Scale for Depression (HRSD-17). Individual patient-level data for at least …

Taxonomic Species Recognition Should Be Consistent, Stephen James O'Brien, Shu-Jin Luo

Biology Faculty Articles

No abstract provided.

Med12 Is A Critical Regulator Of Neural Crest Lineage And Nervous System Myelination, Fatma Betul Aksoy Yasar

Dissertations and Theses (Open Access)

The Mediator complex (MED) is a multi-subunit protein complex integral to the eukaryotic transcription machinery. MED12 is a Cdk8- regulatory kinase module subunit directly implicated in human disease and is genetically altered in neurological disease and cancer. Numerous attempts at generating an in vivo system to study the role of Med12 failed due to embryonic lethality associated with germline or developmental disruption of Med12 gene. To understand the cellular and molecular processes associated with its role in disease, we generated multiple mouse models with targeted depletion of MED12 in distinct cellular lineages. Our genetically engineered models with induced and conditional …

Bobcat Identification, Abundance, And Behavior At Road Mitigation Structures In South Texas, Victoria Hanley

Theses and Dissertations

Highways in South Texas fragment the landscape and result in high road mortality rates for the endangered US ocelot (Leopardus pardalis). In response, the Texas Department of Transportation constructed nine wildlife crossing structures (WCS) on Farm-to-Market (FM) 106 which adjoins Laguna Atascosa National Wildlife Refuge in Cameron County, Texas. With ocelots being rare and elusive, bobcats (Lynx rufus) are often used as a surrogate species to study felid behavior at road mitigation structures in South Texas. Bobcats have unique markings which allow them to be individually identified. Three methods of individual identification of bobcats using camera …

The Role Of Lncrna Uca1 In Colorectal Cancer Progression And Metastasis, Sophia M. Leslie

Theses and Dissertations

Colorectal cancer (CRC) is the primary cause of cancer-related deaths worldwide, and it is the third most common cancer diagnosed in adults in the United States according to the American Cancer Society. We have identified lncRNA UCA1, a poor prognosis marker in CRC, responsible for increased proliferation and upregulating key genes related to glucose metabolism. Recombinant cell lines were generated to overexpress and knockdown UCA1 to establish the phenotype and to investigate the properties endowed in metastasis. A vital aspect of the metastatic progression is when the cell survives detachment from the extracellular matrix. At 36 hours of anchorage-independent stimulation, …

Sequencing And Functional Characterization Of A Latrodectus Geometricus Defensin, Lg-Defensin, Jacklyn Victoria Thompson

Electronic Theses and Dissertations

Defensins are small, cysteine-rich, immune related proteins described to be the most widespread family of invertebrate antimicrobial peptides. Presently, there is limited information regarding spider defensins. In this study, a new defensin peptide was described in Latrodectus geometricus, Lg-defensin. The full open reading frame was resolved using RACE and de novo assembly, followed by a bioinformatical analysis and phylogenetic alignment to determine conserved sequence patterns and structural similarities to other defensins. Next, the functionality of Lg-defensin was determined using in vitro RNA interference to assess what impact silencing had on the spider’s ability to control a gram-positive and gram-negative bacterial …

Analysis Of Per Capita Contributions From A Spatial Model Provides Strategies For Controlling Spread Of Invasive Carp, Donald R. Schoolmaster Jr., Alison A. Coulter, Jahn L. Kallis, Donald C. Glover, John M. Dettmers, Richard A. Erickson

Natural Resource Management Faculty Publications

Metapopulation models may be applied to inform natural resource management to guide actions targeted at location-specific subpopulations. Model insights frequently help to understand which subpopulations to target and highlight the importance of connections among subpopulations. For example, managers often treat aquatic invasive species populations as discrete populations due to hydrological (e.g., lakes, pools formed by dams) or jurisdictional boundaries (e.g., river segments by country or jurisdictional units such as states or provinces). However, aquatic invasive species often have high rates of dispersion and migration among heterogenous locations, which complicates traditional metapopulation models and may not conform to management boundaries. Controlling …

Germination And Early Seedling Growth Attributes Of Rice (Oryza Sativa L.) Cultivars Under Different Salinity (Nacl) Stresses [Research Note], Shahid Hussain, Shuli Liu, Rui Zhang, Yinglong Chen, Altaf Adil, Qigen Dai

The Philippine Agricultural Scientist

This study investigated the impact of different salinity stresses [0, 6.4, 12.8, 19.2, and 25.6 dS/m (NaCl)] on the final germination percentage, speed of germination, germination capacity, shoot and root length, seedling vigor index, and total plant dry weight of ten rice cultivars at germination and early seedling stages. Salinity stress significantly decreased the final germination percentage, speed of germination, germination capacity, shoot and root length, and seedling dry weight in all cultivars, with the most severe effects from level 19.2 and 25.6 dS/m NaCl. Rice cultivars were classified as tolerant, moderately tolerant, moderately susceptible, and susceptible based on reduction …

Synthesis And Characterization Of Nanozeolite From Sugarcane Bagasse Ash And Its Nutrient Loading Potential, Quincy Ybañez, Pearl Sanchez, Marcial Ii Buladaco, Joy Eloiza Rosales

The Philippine Agricultural Scientist

Sugarcane bagasse, a known agricultural waste product in the Philippines with 88% silica (SiO₂) content based on elemental analysis, is a viable starting material for zeolite synthesis. In this study, silica derived from sugarcane bagasse ash (SBA) collected from La Carlota, Negros Occidental, Philippines, was extracted via an alkaline extraction method using 2M NaOH and precipitation with 5 N H₂SO₄ under constant stirring at elevated temperature. Sodium aluminate was added as aluminum source then subjected to ageing at 80°C for 72 h to form the clinoptilolite-type zeolite crystals. Characterization using Scanning Electron Microscopy – Energy …

Rheological Properties And Postharvest Behavior Of Onion (Allium Cepa L. 'Superex') Subjected To Different Preharvest Treatments To Control Armyworm [Spodoptera Exigua (Hubner Lepidoptera: Noctuidae], Daphne Cassandra Gonzales, Ryan Anthony Lualhati, Kim John Nate, Cielo Azul Dumlao, Emmanuel Amatorio, Elda Esguerra, Bonifacio Cayabyab

The Philippine Agricultural Scientist

Heavy reliance on synthetic pesticides has led to insecticide resistance of pests such as the onion armyworm, leading to the use of alternative strategies such as biopesticides. While studies on the efficacy of these biopesticides on pest control and yield exist, their subsequent effects on the quality and postharvest behavior of bulb onions during cold storage need to be determined. Allium cepa L. ‘Superex’ yellow type onion plants were subjected to preharvest treatments of microbial (NPV crude), botanical (neem oil), synthetic insecticide (chlorfenapyr), and Farmer’s Practice (control) for the control of onion armyworm (Spodoptera exigua (Hubner)). Bulbs were then …

Evaluation Of Triticale Lines Compared To Wheat Cultivars In Terms Of Agronomic Traits Using Supervised Learning Methods And Multivariate Statistics, Sara Farokhzadeh, Hossein Shahsavand Hassani

The Philippine Agricultural Scientist

The first man-made cereal grain triticale merges the yield and quality potential of wheat with tolerance of rye against environmental stresses. To find the traits with the greatest impact on the superiority of triticale over wheat, four promising triticale lines and six bread wheat varieties were evaluated in a randomized complete block design (RCBD) with three replications in the field of the Agricultural Research Station of Shiraz University, Iran. Attribute weighting algorithms revealed main leaf width (MLW), grain number per spike (GN/S), spikelet number per spike (SN/S), 1000-grain weight (TGW), plant height (PHT), main leaf length (MLL), and grain yield …

Flowering Habit And Photoperiodic Flowering Response Of Stevia (Stevia Rebaudiana Bertoni) In The Philippines, Kennilyn May Balbin-De Luna, Bong Salazar

The Philippine Agricultural Scientist

Stevia is a perennial shrub commercially cultivated for the sweet-tasting compounds known as steviol glycosides (SVglys). SVglys are extracted from the green leaves, thus maintenance of vegetative growth is critical in stevia production. This study was conducted to describe the flowering habit and response of potted stevia plants to different photoperiods under Philippine conditions. Photoperiodic treatments (10, 11, 12, 13, and 14 h) were provided using light chambers. Stevia has both terminal and axillary flowering habits. It has five white florets enclosed in a capitulum and exhibits an opposite branching pattern. Results showed that stevia is a facultative short-day plant …

Potential Of Mechanical Grain Dryers In Addressing Exposure Of Rice Farmers To Weather Risks, Hernaiz Malanon, Zenaida Sumalde

The Philippine Agricultural Scientist

Weather risks brought by prolonged rainfall and cloudy days hamper grain drying operations in the Philippines as majority still employ the sun drying method, which limits the opportunity of rice farmers to earn a higher income. While mechanical dryers are considered an important adaptation mechanism to address weather risks, more research needs to be done on their benefits and their disadvantages. This study adapted the Inter-governmental Panel on Climate Change (IPCC) concept which equates hazard, exposure, and vulnerability to quantify risk. Hazard was assessed in terms of the probability of four straight rainy- or cloudy-day occurrences based on a 30-year …

Nutrient Recovery From Wastewater By A Consortium Of Algae Species For Biofuel Production, Edgardo Ayala

Boise State University Theses and Dissertations

Current energy sources are predominantly petroleum-based and their use increases greenhouse gas (GHG) emissions. As the global population grows, and along with it the demand for energy, there is a need to further develop renewable energy sources to avoid the effects of increasing atmospheric CO₂ concentrations on the climate. Biofuels, a renewable energy source, have gained significant interest as a replacement for petroleum-based fuels due to their environmental benefits and carbon neutrality. Biofuels are expected to make up 9.0% of the total fuel consumption in the U.S. by 2040, up from 7.3% in 2019 [1]. Currently, terrestrial crop-based biofuels …

Understanding And Mitigating The Effects Of Artificial Light On Bats And Nocturnal Arthropods In Grand Teton National Park, Hunter Cole

Boise State University Theses and Dissertations

Bat and insect populations are at risk globally, and identifying factors that may influence bat and insect populations alongside mitigation techniques for anthropogenic factors that may negatively influence these taxa will be crucial for their conservation. To identify landscape characteristics that influence bats throughout Grand Teton National Park, we placed passive acoustic monitors throughout the park in areas with different microhabitat characteristics to identify factors that influence activity. Additionally, we developed a R package, `EcoCountHelper`, to assist wildlife managers in analyzing ecological count data similar to our bat monitoring data. As a demonstration of the package, we conducted a GLMM-based …

The Influence Of Visitors, Habitat, And Methodology On Mexican Spotted Owl (Strik Occidentalis Lucida) Occupancy And Detection In A Remote Canyon Environment, Kirsten Fuller

Boise State University Theses and Dissertations

National Parks across America play an important role in protecting natural resources and providing access to recreation for visitors. However, these goals may come into conflict as visitation rates rise. Grand Canyon National Park in Northern Arizona is one of the most highly visited parks in the United States, with over 6 million visitors a year. Backcountry hiking and camping are popular activities in the park, and many highly visited hiking trails and campgrounds overlap with known breeding areas of a threatened species, Mexican Spotted Owl. In this thesis, I explore the intersection of recreation and wildlife conservation at this …

Dna Origami Scaffold Development For Digital Nucleic Acid Memory, Sarah Elizabeth Kobernat

Boise State University Theses and Dissertations

Recently, DNA nanotechnology has emerged as a promising and rapidly expanding method to utilize nucleic acids as a nanoscale building material. DNA origami is a major structural application of DNA nanotechnology, using DNA to construct two- and three-dimensional shapes. These structures have been employed for a variety of uses including DNA data storage. DNA is a promising material to address the impending shortage of silicon-based storage as data demands increase. There are many sequence-based methods of data storage, but digit Nucleic Acid Memory (dNAM) uses DNA origami as a breadboard and is read by super-resolution microscopy instead. dNAM uses DNA …

Structure, Function, And Immunogenic Applications Of Ab5-Type Adp-Ribosylating Bacterial Toxins, Elise Marie Overgaard

Boise State University Theses and Dissertations

Bacterial mono-ADP-ribosyltransferases (ARTs) catalyze the singular transfer of an ADP-ribose moiety from an NAD+ molecule onto a target molecule. ARTs contain an ancient and highly conserved tertiary structure and have a wide variety of intracellular targets and effects. Some, but not all, bacterial ARTs have an AB₅-type multimeric structure consisting of an enzymatically active subunit non-covalently situated atop of a non-toxic pentamer. The active, or A, subunit of AB₅-type toxins has a catalytic action that contributes to bacterial pathogenicity, and it is sometimes, but not always, an ART. ArtAB is an ART with AB₅-type …

Tibial Compression During Activities Of Daily Living In Young And Older Adults, Elijah Miles Walker

Boise State University Theses and Dissertations

Introduction: Stress fracture, particularly in the tibia, is a growing concern among older adults (greater than 65 years). Older adults may have inherent stress fracture risk from ageing-related changes to their musculoskeletal system. Specifically, older adults reduced ankle neuromuscular function may impair their ability to attenuate repetitive compressive forces experienced during daily locomotor tasks and increase the likelihood of suffering bone damage from decreased bone tissue elasticity. Yet, it is currently unknown if older adults exhibit greater tibial compression than their younger counterparts during locomotor tasks. Purpose: This study sought to quantify tibial compression for older and younger adults when …

Modulation Of Alpha-Crystallin-Membrane Association By Phospholipid Acyl Chain Length And Degree Of Unsaturation, Geraline Trossi-Torres

Boise State University Theses and Dissertations

Cataract is the leading cause of blindness worldwide. The only treatment for cataracts is the surgical removal of the cataractous lens and the replacement of an intraocular lens. With less availability of treatment and low income, the visual damage caused by cataracts can go untreated. The cataract may develop again after surgery, such as posterior capsule opacification. With age and cataracts, α-crystallin, a significant protein of the mammalian eye lens, is progressively associated with the eye lens membrane. The primary association sites of α-crystallin with the membranes are phospholipids. However, it is unclear if phospholipids’ acyl chain length and degree …

Pronghorn Space-Use Ecology In Utah, Veronica A. Winter

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Pronghorn are viewed as a quintessential part of the landscape in the American West. Found only in western North America, pronghorn is a unique species, having historic ranges within prairie, shrubland-steppe, and desert habitat across the continental west. Even though they have been present on this landscape since the last ice age, little is known of pronghorn ecology. There has been growing concern over the impact anthropogenic features, such as development, agriculture, and roads are having on migration, seasonal range conditions, and overall population dynamics. The aim of this thesis is to investigate factors that may be important for pronghorn …

Evaluating Translocation Strategies For Box Turtles In Urbanizing Landscapes, Elizabeth D. Hays

Biological Sciences Undergraduate Honors Theses

Urbanization of landscapes, resulting in habitat degradation, loss, and fragmentation, is a significant contributor to the global decline of reptile biodiversity. Although translocation is a common management strategy for wildlife populations subject to urbanization, the efficacy of reptile translocation studies, including those of box turtles (Terrapene spp.), is highly variable. Hard-release translocation of box turtles has often proven ineffective due to homing attempts and rapid post-translocation movements. Some studies have presented soft-release as a possible method for mitigation of the negative effects of hard-release, yet those studies have also produced mixed results. Therefore, I radiotracked 18 translocated Terrapene carolina …

A Structural Examination Of The Connection Between The Amygdala And Nucleus Accumbens In Adolescents With Clinical Anxiety, Alyssa Griffith

Biological Sciences Undergraduate Honors Theses

The prominence of anxiety disorders in today’s general population is a major public health concern. Advancing research of the underlying pathophysiology of anxiety disorders can lead to the discovery of effective treatment interventions to treat the mental and physical symptoms of anxiety, and thus improve quality of life. This study aimed to examine two brain areas in the limbic and reward systems, the amygdala and Nucleus Accumbens (NAcc), and the structural white matter connection between them. This neural circuit assigns affective valence to environmental stimuli and motivates behavior to avoid potential harm. This study utilized diffusion Magnetic Resonance Imaging (dMRI) …

Survey For Usage Of And Interest In Personalized Nutrition As A Possible Health Approach, Kimberly Garrett

Biological Sciences Undergraduate Honors Theses

Dietary factors can lead to chronic diseases and impact certain health factors such as sleep and mood. Personalized nutrition is based on an individual’s phenotype and genotype to meet specific dietary needs. This approach could be an intervention to benefit an individual’s health. This study surveyed a population to analyze the population’s view on and usage of personalized nutrition. The use of personalized nutrition was minimal, but there was interest within the population. In addition, a relationship between health and mood was found. However, the sample size was a limiting factor for this study. Future directions could include studying why …

Health Effects Of Neonicotinoids On An Agricultural Community, Jocelyn Rodriguez-Paar

Capstone Experience: Master of Public Health

Mead, Nebraska, a small United States agricultural community consisting of approximately 600 people, was accidentally exposed to high amounts of neonicotinoids when AltEn, an ethanol plant that used neonicotinoid coated corn and soybean seeds for biofuel, was discovered not disposing of the toxic byproduct safely. Coincidentally, there was also a leak in their wastewater system that also contaminated the nearby areas. Although neonicotinoids are not considered highly dangerous with low dose exposures, large dose exposures can cause symptoms similarly to nicotine poisonings, such as difficulty breathing, vomiting, nausea, hypertension, tachycardia, and lethargy (Imamura, T. et al., 2010). Neonicotinoid poisoning can …