Life Sciences Commons^™

Isomaltooligosaccharides Sustain The Growth Of Prevotella Both In Vitro And In Animal Models, Junkui Chen, Zhengpeng Li, Xiaofan Wang, Bin Fan, Feilong Deng, Hongwei D. Yu, Xiaolei Ze, Liying Zhu, Yeshi Yin, Yanhong Chen, Jiangchao Zhao, Yunsheng Yang, Xin Wang

Animal Science Faculty Publications and Presentations

The human digestive tract is colonized by trillions of bacterial cells that play important roles in human health and diseases. It is well known that dietary habits are associated with human microbiota enterotypes. However, the factors that determine the enterotype still remain elusive. In this study, it was first examined, via in vitro batch fermentation, how different carbohydrates affect the Bacteroides and Prevotella enterotypes. Among the 11 substrates (fructo-, galacto-, xylo-, manno-, and isomalto-oligosaccharides [IMO] and lactulose, raffinose, starch, inulin [INU], mannitol, and xylitol) tested, IMO, INU, and starch were found to sustain the growth of Prevotella through batch fermentation. …

Spatial Models Can Improve The Experimental Design Of Field-Based Transplant Gardens By Preventing Bias Due To Neighborhood Crowding, Andrii Zaiats, Juan M. Raquena-Mullor, Matthew J. Germino, Jennifer S. Forbey, Bryce A. Richardson, T. Trevor Caughlin

Biology Faculty Publications and Presentations

Field-based transplant gardens, including common and reciprocal garden experiments, are a powerful tool for studying genetic variation and gene-by-environment interactions. These experiments assume that individuals within the garden represent independent replicates growing in a homogenous environment. Plant neighborhood interactions are pervasive across plant populations and could violate assumptions of transplant garden experiments. We demonstrate how spatially explicit models for plant–plant interactions can provide novel insights on genotypes' performance in field-transplant garden designs. We used individual-based models, based on data from a sagebrush (Artemisia spp.) common garden, to simulate the impact of spatial plant–plant interactions on between-group differences in plant …

Wood Turtle (Glyptemys Insculpta) Spatial Ecology, Habitat Selection, And Use Of Restored Oak-Pine Barrens In Northwestern Michigan, Diana Paige Methner

Masters Theses

Habitat fragmentation and loss, predation, and illegal collection are among the many threats plaguing turtle species worldwide which have caused substantial population declines. In the United States and Canada, approximately 38% of turtle and tortoise species are under significant threat. The wood turtle (Glyptemys insculpta) a freshwater turtle species experiencing declines and in 2023, the U.S. Fish and Wildlife Service will determine if this species should be listed under the Endangered Species Act. We studied the spatial ecology of wood turtles in northwestern Michigan, USA to fill knowledge gaps that exist throughout their geographic range. Between May 2021 …

Isolation Of Drug-Sensitive Eimeria Species From Wild Turkey Feces And Development Of A Model Bioshuttle Program For Eimeria Meleagrimitis For Domestic Turkeys, Maria Carolina Trujillo Peralta

Graduate Theses and Dissertations

The objective of this thesis was to determine the anticoccidial profile of Eimeria spp. derived from wild turkeys. These drug-sensitive Eimeria spp. have potential to be used as vaccine candidates to control coccidiosis in commercial turkeys. Chapter two consists of a brief literature review focused on coccidiosis in turkeys. Chapter three addresses the methodologies utilized to isolate, recover, and speciate Eimeria recovered from wild turkey feces in the eastern two-thirds of the United States. From this, we successfully obtained single oocyst-derived stocks for E. meleagrimitis, E. dispersa, E. meleagridis, E. gallopavonis, and E. adenoeides. Chapter four describes the experiment conducted …

Understanding The Potential Utility Of Tamarktm Grain Sorghum, Jacob Alan Fleming

Graduate Theses and Dissertations

Herbicide-resistant crops have been commonly used in corn, cotton, and soybean since the 1990s to control numerous different grass and broadleaf weeds. However, this technology has not been available for grain sorghum producers whom have faced challenges controlling grass weeds. Recently a collaboration between the University of Arkansas and Texas A&M University has resulted in a new bred line of grain sorghum, TamArkTM, which is resistant to ACCase inhibitor herbicides which have been previously used to control grass weeds in broadleaf crops. Multiple studies were conducted to determine the sensitivity of TamArkTM and problematic grass weeds to ACCase inhibitor herbicides, …

The Effect Of Spirulina Platensis Algae Inclusion In Feed Of Commercial Broilers Subjected To Cyclic Heat Stress, Kirsten Shafer

Graduate Theses and Dissertations

The effect of Spirulina platensis inclusion in feed of commercial broilers was evaluated over one experiment. The study aimed to investigate the effects of Spirulina platensis freshwater algae inclusion on live performance and processing characteristics of commercial broilers subjected to daily cyclic heat stress. Day old Ross 708 male broilers were placed into 8 environmentally controlled chambers. At d 21, four chambers remained on a control diet containing no algae (CON) while 4 chambers received a diet that included algae at an inclusion rate of 2.5% (ALG). An 8 hour daily cyclic heat stress (24°C to 36°C) was applied to …

Applying A Material Science Approach To Improve Rice Processing Performance, Michelle Oppong Siaw

Graduate Theses and Dissertations

Rice is typically consumed as whole kernel, which makes the economic value of whole kernels twice that of brokens. Macro-structural defects such as fissures and chalkiness have been recognized to weaken kernel mechanical strength, and thereby increase breakage during milling. However, high percentages of brokens are sometimes recorded even though these defects are controlled prior to milling. It was hypothesized that rice milling characteristics are influenced by endosperm porosity from the structural arrangement of chemical components and by bran thickness and composition. Thus, this study aimed to modify chemical components by applying treatments to alter rice porosity and its associated …

Telehealth-Based Health Coaching Produces Significant Weight Loss Over 12 Months In A Usual Care Setting, Kelly Johnson, Elizabeth Gutierrez, Patricia Dionicio, Jeremy Mcconnell, Rachel Sauls, Michelle Alencar

Kinesiology

Background: Telehealth-based health coaching in a usual care setting has yet to be examined. The purpose of this study was to incorporate the inHealth Lifestyle Therapeutics, Inc.’s Telehealth Enabled Approach to Multidisciplinary Care (TEAM) method within a real-world routine clinical care setting to reduce body weight in obese participants. Materials and Methods: n = 70 participants were recruited for this intervention (Age: 58.1 ± 14.6yrs, BMI: 35.5 ± 7.8 kg/m², 32 males and 38 females). All participants self-selected participation in either the virtual health coaching (VHC) group or usual care (UC) group. VHC participants met with a medical …

Transcriptomic Signal Of Reproductive Modes And Early Speciation In Daphnia Pulex-Pulicaria System, Trung Viet Huynh

Biology Dissertations - Archive

Daphnia have been gaining recognition as a key model system for studies of evolution, ecology, toxicology, and more recently genomics. The defining characteristic of this particular model system is its phenotypic plasticity and its responsiveness to environmental cues. At the same time, the advent of RNA sequencing technology has helped researchers gain new insights on the plasticity of gene expression and its role linking environmental cues with the physiological response mounted by the organism. Although many RNA-seq studies have been conducted in Daphnia, surprisingly no one has recorded the gene expression profile of one of Daphnia’s most famous phenotypic plastic …

Food Security & Urban Agriculture - Considerations For Soil Improvements And Crop Planning, Aaron Sedivy

Doctor of Plant Health Program: Dissertations and Student Research

Urban agriculture provides many benefits, such as energy conservation, rainwater remediation, increased aesthetic and property value, and therapeutic use for physical, cognitive, and emotional wellbeing. Urban agriculture can also provide critical integrity to each pillar of food security: availability, access, utilization, and stability. As urban agricultural activities increase, social interactions will follow in concert. This will provide opportunities for building trust through the two-way communication of contrasting perspectives, cultures, and beliefs using impartial narratives. Growers can use knowledge and understanding gained from these positive social interactions to formulate their personal paradigms for practice and better meet the needs of each …

Image Analysis And Machine Learning In Agricultural Research, Xinzheng Chen

Doctor of Plant Health Program: Dissertations and Student Research

Agricultural research has been a focus for academia and industry to improve human well-being. Given the challenges in water scarcity, global warming, and increased prices of fertilizer, and fossil fuel, improving the efficiency of agricultural research has become even more critical. Data collection by humans presents several challenges including: 1) the subjectiveness and reproducibility when doing the visual evaluation, 2) safety when dealing with high toxicity chemicals or severe weather events, 3) mistakes cannot be avoided, and 4) low efficiency and speed.

Image analysis and machine learning are more versatile and advantageous in evaluating different plant characteristics, and this could …

Adverse Health Impacts On Honey Bee (Apis Mellifera L.) Colonies From A Contaminated Environment And Resources, Rogan Tokach

Department of Entomology: Dissertations, Theses, and Student Research

Honey bees (Apis mellifera L.) continue to experience high annual declines due to a combination of factors including pesticides. Due to increasing agricultural intensification has led to a reliance on systemic pesticide treated seed which can brought back to the colony in pollen and nectar. Treated seed is not subject to the same guidelines as pesticide applications reducing the regulations on the disposal of treated seed. In 2015, an ethanol plant in Mead, Nebraska began processing expired treated seed for ethanol production. Subsequently, the University of Nebraska-Lincoln Bee Lab started seeing 100% colony failure in apiaries surrounding the plant. …

Evaluating The Efficacy Of Germination And Fermentation In Producing Biologically Active Peptides From Pulses, Ashley Newton

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Recently, there has been a profound increase in demand of plant-based proteins, especially pulse proteins. However, pulses contain high concentrations of antinutritional factors that hinder the digestibility of proteins. Processing techniques such as enzymatic hydrolysis, microbial fermentation, and physical processing modulate nutritional quality and functionality of pulses. Processing also releases peptides from parent proteins that exhibit health-beneficial bioactivity against various disease risk factors. In this study, germination, gastrointestinal digestion, and microbial fermentation were evaluated to determine the nutritional quality and release of bioactive peptides during and after processing.

Germination of chickpeas (Cicer arietinum L.) modulated the protein profile, and …

Egestion Rates Of Microplastic Fibres In Fish Scaled To In Situ Concentration And Fish Density, Loren Hou, Rachel Mcneish, Timothy Hoellein

Biology: Faculty Publications and Other Works

Microplastics (particles <5 >mm) are commonly found in aquatic organisms across taxonomic groups and ecosystems. However, the egestion rate of microplastics from aquatic organisms and how egestion rates compare to other rates of microplastic movement in the environment are sparsely documented.We fed microplastic fibres to round gobies (Neogobius melanostomus), an abundant, invasive species in the Laurentian Great Lakes. We conducted two trials where round gobies were fed microplastic-containing food either a single time (1 day) or every day over 7 days.There was no difference in microplastic egestion rates from the 1 day or 7 day feeding trials, suggesting no impact of …

Comparison Of The Reliability Of Four Different Movement Thresholds When Evaluating Vertical Jump Performance, Madeleine Barefoot, Hugh Lamont, J. Chadwick Smith

Kinesiology

Analyzing vertical jumps performed on a force plate can be useful for the strength and conditioning professional in managing neuromuscular fatigue. The purpose of this study was to compare different movement thresholds when analyzing countermovement (CJ) and squat jump (SJ) performance. Twenty-one college-aged participants (9 female, 12 male) performed five CJs and five SJs. Movement initiation was identified when the vertical ground reaction force (VGRF) deviated five standard deviations (5SD), four standard deviations, (4SD), 2.5% of system weight (2.5%SW), and 10% of system weight (10%SW) from their starting position. For CJs, movement was determined when the VGRF deviated either above …

Targeting A Highly Repetitive Genomic Sequence For Sensitive And Specific Molecular Detection Of The Filarial Parasite Mansonella Perstans From Human Blood And Mosquitoes, Nils Pilotte, Tamara Thomas, Michael F. Zulch, Allison R. Sirois, Corrado Minetti, Lisa J. Reimer, Steven A. Williams, Lori J. Saunders

Biological Sciences: Faculty Publications

Background Mansonella perstans is among the most neglected of the neglected tropical diseases and is believed to cause more human infections than any other filarial pathogen in Africa. Based largely upon assumptions of limited infection-associated morbidity, this pathogen remains understudied, and many basic questions pertaining to its pathogenicity, distribution, prevalence, and vector-host relationships remain unanswered. However, in recent years, mounting evidence of the potential for increased Mansonella infection-associated disease has sparked a renewal in research interest. This, in turn, has produced a need for improved diagnostics, capable of providing more accurate pictures of infection prevalence, pathogen distribution, and vector-host interactions. …

Amorphous Silicon Oxynitride Coatings For Craniofacial Implants Stimulate Osteogenesis Via Nrf2 Antioxidant Activity, Neelam Ahuja

Kinesiology Dissertations - Archive

Craniomaxillofacial injuries and defects are functionally and socially debilitating. These defects require immediate attention, and most cases undergo reconstruction surgery to restore the lost tissues. Current treatment strategies such as autologous bone grafts and fixative titanium devices are well established to treat the craniofacial region. But, the limited supply of donor bone and secondary surgery limit the use of autologous bone grafts, and low bioactivity, poor osteointegration of the titanium devices, has led to an increased number of tissue engineering research for the development of newer biomaterials. Bone defects especially in the craniofacial region can have a significant portion of …

Combining Network Modeling And Experimental Approaches To Predict Drug Combination Responses, Deepraj Sarmah

All Dissertations

Cancer is a lethal disease and complex at multiple levels of cell biology. Despite many advances in treatments, many patients do not respond to therapy. This is owing to the complexity of cancer-genetic variability due to mutations, the multi-variate biochemical networks within which drug targets reside and existence and plasticity of multiple cell states. It is generally understood that a combination of drugs is a way to address the multi-faceted drivers of cancer and drug resistance. However, the sheer number of testable combinations and challenges in matching patients to appropriate combination treatments are major issues.

Here, we first present a …

Developmental Effects Of Chronic Low-Level Arsenic Exposure In Mouse Embryonic Stem Cells And In Human Induced Pluripotent Stem Cells, M. Chiara Perego

All Dissertations

Arsenic is an environmental contaminant commonly found in food and drinking water. Exposure to arsenic during embryonic development has been linked to reduced muscle growth, disrupted muscle development and locomotor activity, impaired neurodevelopment, reduced IQ, impaired memory and learning deficits. While the mechanisms responsible for developmental changes following in utero exposure to arsenic are not well known, one possibility is that arsenic might disrupt proper cellular differentiation. Therefore, we aimed to investigate the mechanisms by which arsenic exposure could alter stem cell differentiation into neurons.

First, we continuously exposed P19 mouse embryonic stem (ES) cells to 0.1 μM (7.5 ppb) …

Callery Pear Allelopathy Study, Michaela J. Woods, Jonathan T. Bauer, Dena Schaeffer, Ryan W. Mcewan

Five Rivers MetroParks Collaboration Data Archive

No abstract provided.

Interneuronal Network Model Of Theta-Nested Fast Oscillations Predicts Differential Effects Of Heterogeneity, Gap Junctions And Short Term Depression For Hyperpolarizing Versus Shunting Inhibition, Guillem Via, Roman Baravalle, Fernando R. Fernandez, John A. White, Carmen C. Canavier

School of Medicine Faculty Publications

Theta and gamma oscillations in the hippocampus have been hypothesized to play a role in the encoding and retrieval of memories. Recently, it was shown that an intrinsic fast gamma mechanism in medial entorhinal cortex can be recruited by optogenetic stimulation at theta frequencies, which can persist with fast excitatory synaptic transmission blocked, suggesting a contribution of interneuronal network gamma (ING). We calibrated the passive and active properties of a 100-neuron model network to capture the range of passive properties and frequency/current relationships of experimentally recorded PV+ neurons in the medial entorhinal cortex (mEC). The strength and probabilities of chemical …

Human Loss-Of-Function Variants In The Serotonin 2c Receptor Associated With Obesity And Maladaptive Behavior, Yang He, Bas Brouwers, Hesong Liu, Hailan Liu, Katherine Lawler, Edson Mendes De Oliveira, Dong-Kee Lee, Yongjie Yang, Aaron R Cox, Julia M Keogh, Elana Henning, Rebecca Bounds, Aliki Perdikari, Vikram Ayinampudi, Chunmei Wang, Meng Yu, Longlong Tu, Nan Zhang, Na Yin, Junying Han, Nikolas A Scarcelli, Zili Yan, Kristine M Conde, Camille Potts, Jonathan C Bean, Mengjie Wang, Sean M Hartig, Lan Liao, Jianming Xu, Inês Barroso, Jacek Mokrosinski, Yong Xu, I Sadaf Farooqi

Children’s Nutrition Research Center Staff Publications

Serotonin reuptake inhibitors and receptor agonists are used to treat obesity, anxiety and depression. Here we studied the role of the serotonin 2C receptor (5-HT2CR) in weight regulation and behavior. Using exome sequencing of 2,548 people with severe obesity and 1,117 control individuals without obesity, we identified 13 rare variants in the gene encoding 5-HT2CR (HTR2C) in 19 unrelated people (3 males and 16 females). Eleven variants caused a loss of function in HEK293 cells. All people who carried variants had hyperphagia and some degree of maladaptive behavior. Knock-in male mice harboring a human loss-of-function HTR2C variant developed …

Round Goby (Neogobius Melanostomus) Seasonal Diet, Drift Impacts, And Influence On Stream Connectivity In Great Lakes Tributary Streams, Kyle R. Glenn Mr

Biology Theses

Seasonality, energy recruitment, and food web position dictate the degree of impact fish impose on their surrounding environment. In the Great Lakes, the round goby (Neogobius melanostomus) is known to out-migrate from nearshore habitat to offshore depths in the winter months. However, in Great Lake tributaries, round goby populations were found up to 25 km upstream during winter months, and distance upstream was a predictor of out-migration propensity; populations farthest upstream did not out-migrate. Seasonal resources shaped the diet and condition of these fish thus creating inland populations with different average lengths, diets, and body condition compared to …

Evaluation Of The Selected Nuproxa Feed Additives For Protection Against Lameness And Improving The Wellbeing Of Broilers In The Lameness Challenge Model, Abigail Fanous

Biological Sciences Undergraduate Honors Theses

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a form of bacteria that causes lameness in broiler chickens and is a common concern within the poultry industry. This easily transmissible disease can quickly take over entire livestock of chickens leading to an animal welfare issue. Due to this, it is a pressing issue that must be solved in order to protect the wellbeing of broiler chickens and ensure viable livestock. Nuproxa is a company that specializes in chicken feed in which we used to test different amounts of Panbonis, a dietary supplement, in its effectiveness in protecting broiler chickens against BCO lameness. …

Exploring Stress In Adolescents Regarding The Covid-19 School Closure, Anna Beck

The Eleanor Mann School of Nursing Undergraduate Honors Theses

Background: The purpose of this literature review, is to explore the stress adolescents faced during the times of the COVID-19 pandemic. This includes the rates of stress, other psychological consequences, such as depression and anxiety, as well as factors that could have contributed to the development of stress and those that could have helped relieve feelings of distraught.

Purpose: To create a more holistic approach in nursing care, finding reasonings behind the creation of stress in the young population, as well as methods to alleviate, can help patient satisfaction and support.

Method: A literature review was conducted over 20 peer …

Channel Catfish - Ictalurus Punctatus, Jason E. Rosales, David William Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium total length is 110.81 mm, [CHANNEL CATFISH , Ictalurus punctatus} collected from Canal in Davie on November 18th, 2022 by Zachary

Improved Computational Prediction Of Function And Structural Representation Of Self-Cleaving Ribozymes With Enhanced Parameter Selection And Library Design, James D. Beck

Boise State University Theses and Dissertations

Biomolecules could be engineered to solve many societal challenges, including disease diagnosis and treatment, environmental sustainability, and food security. However, our limited understanding of how mutational variants alter molecular structures and functional performance has constrained the potential of important technological advances, such as high-throughput sequencing and gene editing. Ribonuleic Acid (RNA) sequences are thought to play a central role within many of these challenges. Their continual discovery throughout all domains of life is evidence of their significant biological importance (Weinreb et al., 2016). The self-cleaving ribozyme is a class of noncoding Ribonuleic Acid (ncRNA) that has been useful for …

Anticipating The Impacts Of The Social, Political, And Biophysical Landscape On Long-Term Connectivity For Reintroduced Plains Bison, Jamie Ann Faselt

Boise State University Theses and Dissertations

Intense anthropogenic pressures on the natural environment have created the need for implementing strategies that promote or restore habitat connectivity. The ability for animals to move between habitat patches allows animals to find mates, access resources, and shift their range in response to the changing climate and ensures that ecological and evolutionary processes persist. Connectivity conservation typically focuses on biophysical barriers to animal movement, but for many species reintroductions, establishing and maintaining connectivity often requires overcoming both ecological and socio-political barriers. Despite the need to navigate complex socio-political landscapes to implement connectivity conservation plans, datasets depicting those conditions are rarely …

The Effects Of Mental Training On Acute Psychophysiological Stress Responses In Endurance Athletes, Shelanda Antonia Maria Kujala

Boise State University Theses and Dissertations

Introduction: In sports, pre-competition stress responses can influence performance. Mental skills training is a strategy used to successfully mitigate stress responses and positively impact performance. Psychological (e.g., anxiety) and physiological (e.g., cortisol) stress responses are not often measured in a single study, providing an incomplete picture of athlete experiences. When researchers have measured these constructs together, studies have excluded endurance athletes and ways to effectively buffer stress responses. Purpose: The current study had two aims. 1. How will athlete’s perceptions of stress and physiological markers of stress be related to each other? 2. How will athlete’s perceptions of stress and …

Integrative Physiology-Coupled Pilot-Centered Flight Simulation, Shawn C. Harrison

Electrical & Computer Engineering Theses & Dissertations

Maintaining the pilot’s physiological performance envelope within the limits of human capabilities may be crucial for avoiding hazardous physiological episodes in fighter aircraft that compromise safety. The main physiological episode of interest is impaired pilot respiration, better known as hypoxia caused by a high fraction of inspired oxygen (FiO₂) at high altitudes and variation in accelerative gravitational forces (g-forces). Integrated into fighter aircraft is an Onboard Oxygen Generating System (OBOGS) developed to mitigate the necessity of gaseous and liquid oxygen cannisters [1]. OBOGS act as a life support in hypoxic environments by providing oxygen-rich air, thereby oxygenating the …