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Full-Text Articles in Life Sciences

Using Proteomics To Discover New Connections In The Arabidopsis Circadian Clock, Maria Lynn Sorkin Dec 2022

Using Proteomics To Discover New Connections In The Arabidopsis Circadian Clock, Maria Lynn Sorkin

Arts & Sciences Graduate Student Theses and Dissertations

The plant circadian clock is an endogenous timekeeping mechanism that uses daylength and temperature cycles to synchronize internal physiology with the external environment. Much of our understanding of the clock in the model plant Arabidopsis thaliana comes from genetic approaches. In this thesis, I use affinity purification coupled with mass spectrometry (APMS) to identify protein-protein interactions for core clock components on a proteomic scale. I developed and optimized a protocol to perform APMS on a core set of circadian clock proteins: CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), LATE ELONGATED HYPOCOTYL (LHY), PSEUDORESPONSE REGULATOR 5 (PRR5), PRR7, PRR9, TIMING OF CAB 1 …

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Bioinformatics And Cancer Genomics Approaches To Advance Precision Medicine And Elucidate Tumor Mutational Landscapes, Kelsy Cotto Dec 2022

Bioinformatics And Cancer Genomics Approaches To Advance Precision Medicine And Elucidate Tumor Mutational Landscapes, Kelsy Cotto

Arts & Sciences Graduate Student Theses and Dissertations

Next-generation sequencing of DNA and RNA continues to be integrated into pre-clinical and clinical research. However, challenges still remain that impede the translation of findings into an improved understanding of human diseases or clinically actionable alterations. The projects described in this dissertation start with compiling efforts from experts who have identified druggable genes within the human genome, followed by in-depth analyses which characterize the pan-cancer landscape of splice-associated mutations and noncoding, regulatory mutations across multiple subtypes of breast cancer. In Chapters 2 and 3, substantial efforts were made to update the content and the user experience for the drug-gene interaction …

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Characterizing Complex Affective Processing Across Age And In Relation To Social Anxiety Symptoms, M. Catalina Camacho Dec 2022

Characterizing Complex Affective Processing Across Age And In Relation To Social Anxiety Symptoms, M. Catalina Camacho

Arts & Sciences Graduate Student Theses and Dissertations

Humans require a shared understanding of others’ emotions for adaptive social functioning. For instance, if someone tells a joke and another person laughs, we assume the laughing person was happy because we have a shared concept for such positive interactions. Children develop and refine these concepts as they grow up, but the underlying neural processing that supports this development is unknown. Identifying the neurodevelopment underlying emotion processing could provide important insight to emotional disorders. For example, social anxiety is associated differences in the detection and interpretation of negative or neutral emotional cues, but the underlying neurodevelopment of this behavior is …

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Host Development And Microbiota Influences On Norovirus Infection, Elizabeth Alexandra Kennedy Dec 2022

Host Development And Microbiota Influences On Norovirus Infection, Elizabeth Alexandra Kennedy

Arts & Sciences Graduate Student Theses and Dissertations

The mammalian gut is a complex organ which changes over the course of development and is influenced by the presence of a dense community of gut-resident microbes. Enteric viruses infect the gastrointestinal tract in the context of the commensal microbiota and host immunity. Here, we dissect the influence of host developmental stage and the gut microbiota in norovirus infection. From this work, we determine that murine norovirus (MNoV) infection in early life is restricted by an absence of the target MNoV cell tuft cells and intact interferon (IFN) signaling. However, viral inoculum taken up by non-specific endocytosis in the neonatal …

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Review Of The Physical And Chemical Properties Of Seagrass Soils, Nerea Piñeiro-Juncal, Oscar Serrano, Miguel Ángel Mateo, Elena Diaz-Almela, Carmen Leiva-Dueñas, Antonio Martinez-Cortizas Dec 2022

Review Of The Physical And Chemical Properties Of Seagrass Soils, Nerea Piñeiro-Juncal, Oscar Serrano, Miguel Ángel Mateo, Elena Diaz-Almela, Carmen Leiva-Dueñas, Antonio Martinez-Cortizas

Research outputs 2022 to 2026

Seagrasses are a polyphyletic group of angiosperms that colonized marine environments more than 30 million years ago and currently inhabit coastal soft and rocky substrates in all continents except Antarctica. Due to their evolution from terrestrial plants, seagrasses have belowground organs that interact with the substrate, transforming it through chemo-physical processes analogous to terrestrial soil formation. Although seagrass substrates provide valuable ecosystem services including carbon and coastal stabilization, they have been largely regarded as sediments by marine scientists and neglected in soil science research. However, over the last decades, the increasing interest in carbon accumulation by seagrasses has generated multiple …

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Korelasi Hasil Padi Sawah Dengan Sulfur Tersedia Dan Sifat Kimia Tanah Sawah, Purwandaru Widyasunu, Rosi Widarawati Dec 2022

Korelasi Hasil Padi Sawah Dengan Sulfur Tersedia Dan Sifat Kimia Tanah Sawah, Purwandaru Widyasunu, Rosi Widarawati

Jurnal Kultivasi

Pemberian belerang dapat digunakan untuk mengatur sifat kimia tanah sawah. Penelitian ini bertujuan untuk mengetahui: (1) distribusi unsur hara S tanah di lahan sawah yang digunakan untuk budidaya tanaman padi sawah, (2) sifat kimia tanah meliputi pH H2O, pH KCl, Potensial redoks dan DHL (Daya Hantar Listrik) pada lahan sawah, (3) hubungan S-tersedia, sifat kimia tanah, dengan hasil tanaman. Penelitian dilaksanakan di lahan sawah di Sub DAS Serayu Hilir Wilayah Kecamatan Maos, Kabupaten Cilacap dengan ketinggian tempat 8 m dpl dan Laboratorium Ilmu Tanah, Fakultas Pertanian, Universitas Jenderal Soedirman pada Juni hingga September 2021. Penelitian ini dilakukan dengan metode survei …

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Extensive Behavioral Phenotyping Of Williams Syndrome Locus Relevant Mouse Models To Assess Contributions Of Oxytocin And Gtf2ird1, Kayla Rose Nygaard Dec 2022

Extensive Behavioral Phenotyping Of Williams Syndrome Locus Relevant Mouse Models To Assess Contributions Of Oxytocin And Gtf2ird1, Kayla Rose Nygaard

Arts & Sciences Graduate Student Theses and Dissertations

The Williams Syndrome Critical Region (WSCR) at chromosome 7q11.23 provides a unique opportunity to untangle the relationship between genotype and phenotype in complex behaviors, from fear and anxiety to sociability and sensorimotor processing. Copy number variations (CNVs) in this region result in two syndromes, Williams Syndrome (WS) and Duplication 7q11.23 Syndrome (Dup7), which display phenotypes that may align, indicating a common disruption of a system, or diverge, reflecting an underlying gene dosage-dependent effect. While case studies of atypical deletions resulting in WS have implicated telomeric genes Gtf2ird1 and Gtf2i in the cognitive and behavioral profiles of WS, proving causation requires …

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Circadian Circuitry And Glioblastoma: Interactions With Behavior, Carcinogenesis, And Clinical Practice., Anna R. Damato Dec 2022

Circadian Circuitry And Glioblastoma: Interactions With Behavior, Carcinogenesis, And Clinical Practice., Anna R. Damato

Arts & Sciences Graduate Student Theses and Dissertations

Glioblastoma (GBM) is the most common primary adult brain tumor diagnosed, with a 15-month prognosis using current standard of care treatment which includes chemotherapy with temozolomide (Temodar, TMZ). Chronotherapy, the practice of considering time of day in treating a disease, has been shown to improve outcome in several cancers, but had not been applied to GBM. Previous work in the lab on a cultured murine mesenchymal GBM cell line showed a time-of-day dependent maximum in sensitivity to TMZ corresponding with the daily peak of Bmal1 and trough of Per2 expression, two core clock genes. Based on this finding, we sought …

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Sik3 & Wnk Signals Through Fray To Regulate Glial K+ Buffering And Seizure Susceptibility In Drosophila Models Of Hyperexcitability, Lorenzo Laronn Lones Dec 2022

Sik3 & Wnk Signals Through Fray To Regulate Glial K+ Buffering And Seizure Susceptibility In Drosophila Models Of Hyperexcitability, Lorenzo Laronn Lones

Arts & Sciences Graduate Student Theses and Dissertations

K+ homeostasis is important for maintaining healthy, physiological levels of neuronal activity. Glial cells play a central role in maintaining homeostatic ion gradients. In previous work from our lab, we unravel a glial K+ buffering program that is centered on a key kinase, salt-inducible kinase 3 (SIK3). SIK3-HDAC4 signaling in glial regulates the transcription of channels and transporters involved in water and ion transport. Defects in this pathway lead to peripheral nerve edema, neuronal hyperactivity, and seizure sensitivity. In an hyperexcitability mutant, eag Shaker, we show this pathway is downregulated and genetic activation suppresses seizure behavior. In this thesis, I …

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Phenolic Compounds Profile And Antioxidant Activities Of Different Fractions Of Tartary Buckwheat, Chen Yue, Zhu Yong, Qin Li-Kang Dec 2022

Phenolic Compounds Profile And Antioxidant Activities Of Different Fractions Of Tartary Buckwheat, Chen Yue, Zhu Yong, Qin Li-Kang

Food and Machinery

Objective: This study aimed to investigate the phenolic compounds profile and antioxidant activities of different fractions of Tartary buckwheat. Methods: Tartary buckwheat was ground and sieved into shell, bran and powder. Phenolic compounds in tartary buckwheat were extracted with 70% ethanol, and phenolic compounds composition and antioxidant activity of the extract were analyzed. Results: In Tartary buckwheat bran, the total phenolics and flavonoids were (3 042±31) mg GAE/100 g·DW and (5 290±233) mg RE/100 g·DW, accounting for 60.8%, 67.7% of the total Tartary buckwheat, respectively. Rutin was the dominant phenolic compounds in bran extract, and the content …

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Effects Of Lotus Leaf Powder On Physicochemical Properties Of Flour And Crisp Biscuits, Wang Feng-Lei Dec 2022

Effects Of Lotus Leaf Powder On Physicochemical Properties Of Flour And Crisp Biscuits, Wang Feng-Lei

Food and Machinery

Objective: The effects of lotus leaf coarse powder and lotus leaf superfine powder on the physical and chemical indexes of flour and its application in crisp biscuits were studied in this paper, and explored the suitable amount of lotus leaf coarse powder and lotus leaf superfine powder added in crisp biscuits. Methods: Farinograph, extensograph, wet gluten tester, and falling number tester were used to determine the effects of lotus leaf powder on the physical and chemical indexes of flour. The hardness of the dough and the shear force of the crisp biscuit were measured by the texture analyzer, …

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Determination Of Duck Meat Adulteration Ratio In Pork Stuffing, Fei Ying-Min, Pan Lei, Ni Xue, Zhang Gen-Sheng Dec 2022

Determination Of Duck Meat Adulteration Ratio In Pork Stuffing, Fei Ying-Min, Pan Lei, Ni Xue, Zhang Gen-Sheng

Food and Machinery

Objective: This study aimed to determine the proportion of duck adulteration in pork stuffing by gas chromatography. Methods: Based on the analysis of characteristic fatty acid types as well as contents in different meats by gas chromatography, the standard fingerprint profiles of pork and duck were established manually, and the chromatograms of duck adulteration samples with 10%, 30%, 50% and 70% adulteration in pork stuffing were analyzed. The characteristic fatty acids of duck-pork were finally selected as lauric acid, stearic acid, trans Behenic acid, and linoleic acid. The adulteration experiment was simulated to establish the prediction regression models …

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Detection Of Defective Shrimp And Optimization For Key Parameters In Multi-Channel Automatic Elimination System, Zeng Ling-Xiao, Liu Zi-Hao, Yan Zhi-Peng, Zhao Kun, Liu Hao Dec 2022

Detection Of Defective Shrimp And Optimization For Key Parameters In Multi-Channel Automatic Elimination System, Zeng Ling-Xiao, Liu Zi-Hao, Yan Zhi-Peng, Zhao Kun, Liu Hao

Food and Machinery

Objective: When inspecting the appearance and quality of Litopenaeus Vannamei, the system can realize the automatic elimination of defective shrimps efficiently. Methods: Constructed an automatic shrimp elimination equipment and quality inspection. Structured an algorithm to extract shrimps’ morphological feature, complete the clarification of normal and defective shrimps. Realizing defective shrimps’ elimination by blowing them from the side to middle. Through the analysis of internal stress of shrimp, we calculated the correlation between blowing volume and moving distance. Moreover, the relationships among gass output volume, shrimps’ moving distance and speed had been optimized. Results: With the increasing of …

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Real-Time Classification Method For Liquor Hops Based On Deep Learning, Liu Zhi-Ping, Cui Ke-Bin Dec 2022

Real-Time Classification Method For Liquor Hops Based On Deep Learning, Liu Zhi-Ping, Cui Ke-Bin

Food and Machinery

Objective: To solve the subjectivity and instability of the traditional Baijiu picking method " liquor-receiving according to liquor hop", and the problem that the existing machine vision hops classification method is difficult to meet the real-time classification. Methods: The lightweight YOLOv5 takes YOLOv5s as the initial model, uses the K-mean clustering anchor box to replace the default anchor box to improve the model detection accuracy and stability, uses the shufflenetv2 network to replace the YOLOv5s backbone network for feature extraction, so as to achieve the purpose of lightweight model, and adds the CBAM attention mechanism to make the …

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Automatic Classification Of Banana Ripeness Based On Deep Learning, Wang Ling-Min, Jiang Yu Dec 2022

Automatic Classification Of Banana Ripeness Based On Deep Learning, Wang Ling-Min, Jiang Yu

Food and Machinery

Objective: To classify banana ripeness quickly and accurately. Methods: Collect the bananas images of different maturity and establish gallery, using a variety of different neural networks as a classifier, banana feature extracting by migration study classifying banana six maturity level, access to the most suitable for banana maturity classification network model, network model, based on the improved and easily banana maturity real-time detection interface design, Finally, the feasibility and practicability of the model were verified. Results: AlexNet model was most suitable for banana maturity classification with the highest accuracy of 95.56%. AlexNet model was improved by modifying …

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Preparation And Application Of Maillard Reaction Flavors Based On Cigar Tobacco Leaf Residues, Xu Xiu-Juan, Wang Ai-Xia, Hu Shao-Dong, Shi Qing-Zhao, Yang Chun-Qiang, Ma Ji Dec 2022

Preparation And Application Of Maillard Reaction Flavors Based On Cigar Tobacco Leaf Residues, Xu Xiu-Juan, Wang Ai-Xia, Hu Shao-Dong, Shi Qing-Zhao, Yang Chun-Qiang, Ma Ji

Food and Machinery

Objective: To solve the problem of tobacco leaf residues produced during the production of tobacco extracts. Methods: Maillard reaction was carried out using aqueous extracts from cigar tobacco leaf residues as raw materials, and the effects of amino acid dosage and reaction time on the chemical composition of the Maillard reaction products and the sensory quality of cigarettes were systematically investigated. The relationship between the 18 components of the Maillard reaction product and the sensory index of cigarettes was also investigated. Results: ① Based on sensory effects, the optimal reaction conditions for the Maillard reaction based on …

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Research Progress On The Sources And Detection Methods Of Determination Of Benzo-Α-Pyrene In Aquatic Products, Xia Hong, Liu Li, Peng Xi-Tian, Peng Li-Jun, Lin Chun Dec 2022

Research Progress On The Sources And Detection Methods Of Determination Of Benzo-Α-Pyrene In Aquatic Products, Xia Hong, Liu Li, Peng Xi-Tian, Peng Li-Jun, Lin Chun

Food and Machinery

This review summarized the source, properties, as well as the harm of benzo-α-pyrene exposure to aquatic products. The commonly used instrumental analysis methods for the detection of benzo-α-pyrene were also discussed, and the future research direction of benzo-α-pyrene detection was prospected.

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A Deep Learning-Based Model For Plant Lesion Segmentation, Subtype Identification, And Survival Probability Estimation, Muhammad Shoaib, Babar Shah, Tariq Hussain, Akhtar Ali, Asad Ullah, Fayadh Alenezi, Tsanko Gechev, Farman Ali, Ikram Syed Dec 2022

A Deep Learning-Based Model For Plant Lesion Segmentation, Subtype Identification, And Survival Probability Estimation, Muhammad Shoaib, Babar Shah, Tariq Hussain, Akhtar Ali, Asad Ullah, Fayadh Alenezi, Tsanko Gechev, Farman Ali, Ikram Syed

All Works

Plants are the primary source of food for world’s population. Diseases in plants can cause yield loss, which can be mitigated by continual monitoring. Monitoring plant diseases manually is difficult and prone to errors. Using computer vision and artificial intelligence (AI) for the early identification of plant illnesses can prevent the negative consequences of diseases at the very beginning and overcome the limitations of continuous manual monitoring. The research focuses on the development of an automatic system capable of performing the segmentation of leaf lesions and the detection of disease without requiring human intervention. To get lesion region segmentation, we …

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Emerging Insights Into A Role For Topoisomerase Iib And Methyl-Cpg Binding Protein 2 In Regulating Neuronal Transcription, Sabin Nettles Dec 2022

Emerging Insights Into A Role For Topoisomerase Iib And Methyl-Cpg Binding Protein 2 In Regulating Neuronal Transcription, Sabin Nettles

Arts & Sciences Graduate Student Theses and Dissertations

The mammalian brain has unparalleled diversity of cell types with distinct molecular, morphological, connectional and functional properties. The specialization of these cells occurs during development through a series of spatially and temporally controlled changes in gene expression that are critical for proper assembly and function. Neurons in particular have complex and elaborate gene regulatory systems which allow specific combinations of genes to be expressed at distinct levels and at discrete developmental stages, giving rise to heterogenous neuronal subtypes. Identifying unique neuronal gene regulatory factors that contribute to the dynamic changes in gene expression are critical to understanding the molecular mechanism …

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System Shock: Using -Omics Data To Characterize Biological Remodeling In The Microbiome, Resistome, And Transcriptome Of Bacteria, Winston Eugene Anthony Dec 2022

System Shock: Using -Omics Data To Characterize Biological Remodeling In The Microbiome, Resistome, And Transcriptome Of Bacteria, Winston Eugene Anthony

Arts & Sciences Graduate Student Theses and Dissertations

The human experience is indelibly linked to microbial life. So much so, that the holobiont theory has been coined to define the assemblage of host and microbe as a discrete ecological unit. Perturbation of the commensal microbiome can create niche space for pathogens, which through the concomitant rise of antimicrobial resistance represent an ever-evolving danger. In Chapter 2 of this dissertation, I describe the results of an interventional study designed to directly perturb the healthy gut microbiome and observe the dynamics of taxonomic composition and functional recovery. I observe significant decreases after 5 days of antibiotic treatment in the gold …

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Insights Into The Non-Osmoregulatory Function Of A Pollen-Specific Mechanosensitive Ion Channel, Kari Miller Dec 2022

Insights Into The Non-Osmoregulatory Function Of A Pollen-Specific Mechanosensitive Ion Channel, Kari Miller

Arts & Sciences Graduate Student Theses and Dissertations

Pollen, the male gametophyte of flowering plants, delivers the sperm cells to the ovule to carry out sexual reproduction. During this process, the pollen grain undergoes dramatic physical changes. Survival requires careful control of cell mechanics, particularly the balance between protoplast expansion and cell wall resistance. One control mechanism is the use of a mechanosensitive (MS) ion channel, MscS-Like (MSL)8. This pollen-specific protein was previously shown to be essential for pollen survival during hydration and was proposed to function as a tension-gated osmoregulator. However, direct proof of osmoregulation during initial hydration has not yet been found. In fact, studies of …

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Contributions Of Specific Retinal Circuits And Their Respective Projections To Visual Behaviors, Jenna Mackenzie Krizan Dec 2022

Contributions Of Specific Retinal Circuits And Their Respective Projections To Visual Behaviors, Jenna Mackenzie Krizan

Arts & Sciences Graduate Student Theses and Dissertations

The survival of a species is inextricably linked to its ability to successfully navigate and interact with its surroundings, whether to seek safety from predators or gain sustenance from prey. Both functions are performed by mice, guided by vision, and rely on intricate processing in the retina and subcortical targets in the brain. This dissertation addresses how specific features of the visual environment and specific retinal ganglion cell circuits that sample a particular space in the visual environment are used to guide efficient predation in mice.Recent studies have begun to link the ability to detect, track, and ultimately capture prey …

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The Impact Of Nucleolar Stress On Thymocyte Development And T Cell Acute Lymphoblastic Leukemia Transformation, Joseph Ryan Krambs Dec 2022

The Impact Of Nucleolar Stress On Thymocyte Development And T Cell Acute Lymphoblastic Leukemia Transformation, Joseph Ryan Krambs

Arts & Sciences Graduate Student Theses and Dissertations

Increased Notch signaling is thought to be central to T-ALL pathogenesis, with activatingmutations of NOTCH1 in 60% of cases, and Notch pathway activation due to mutations in FBXW7 in an additional 15% of cases. Increased Notch signaling results in markedly increased ribosome biogenesis, inducing a negative feedback loop called the nucleolar stress pathway. This is an evolutionarily conserved homeostatic pathway that is triggered by excessive ribosome biogenesis that serves to limit rRNA synthesis, protein translation, and ultimately cell growth. CDKN2A, encoding both ARF and INK4A, plays a key role in the nucleolar stress pathway. Activation of ARF and INK4A inhibits …

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Making Emotions Trustworthy, Xiaoyu Ke Dec 2022

Making Emotions Trustworthy, Xiaoyu Ke

Arts & Sciences Graduate Student Theses and Dissertations

I investigate ways by which agents can make their emotions more trustworthy from a perspective that’s informed by recent psychological research on the nature of emotion regulation. This dissertation is a collection of three articles, each addressing one type of cases of untrustworthy emotions in a particular context. Chapter one (Developing Appropriate Emotions) concerns emotions that are untrustworthy because they are irrelevant to the moral or epistemic judgements at hand. By situating the problem in the context of a situationist challenge to neo-Aristotelian virtue ethics, I argue that we can address this problem by developing ameliorative abilities such as emotion …

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Generation, Characterization, And Treatment Of Functional Zebrafish Models For Musculoskeletal Disorders, Julia Nicole Whittle Dec 2022

Generation, Characterization, And Treatment Of Functional Zebrafish Models For Musculoskeletal Disorders, Julia Nicole Whittle

Arts & Sciences Graduate Student Theses and Dissertations

Musculoskeletal disorders in humans present with a broad range of phenotypes that vary in severity, onset, and genetic cause. The musculoskeletal system is comprised of complex and diverse tissues that make up the majority of total body mass. Thus, mutations in many different genes with differing structure and function can produce phenotypically similar developmental and morphological defects in the muscle and skeleton. In addition, the muscular and skeletal systems develop non-autonomously, wherein development and function of each will affect development of the other. Thus, mutations in genes responsible for development of this complex system have the potential to influence morphogenesis …

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Single-Cell Massively Parallel Reporter Assays, Siqi Zhao Dec 2022

Single-Cell Massively Parallel Reporter Assays, Siqi Zhao

Arts & Sciences Graduate Student Theses and Dissertations

Our understanding of gene regulation needs to be generalizable as well as specific. A generalizable understanding enables us to transfer our knowledge of one gene to another, and the specificity allows us to understand the precise regulation of gene expression through development. One framework that confers generalizability and specificity is the hierarchical and modular model of gene regulation. To test whether gene regulation is hierarchical and modular requires methods to systematically analyze how different factors collectively control gene expression. This thesis describes the development of two functional genomics methods at single-cell resolution. These methods systematically examined how cellular contexts, chromatin …

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A Structural Perspective On Neutralizing Antibodies To Flaviviruses And Coronaviruses, John Michael Errico Dec 2022

A Structural Perspective On Neutralizing Antibodies To Flaviviruses And Coronaviruses, John Michael Errico

Arts & Sciences Graduate Student Theses and Dissertations

The presence of neutralizing antibodies typically correlates strongly with protection from infection, particularly for RNA viruses; elicitation of neutralizing antibody responses is thus the primary goal of most vaccines, and monoclonal antibodies (mAbs) can be leveraged as potent therapeutics. Thus, understanding the properties of individual antibodies that comprise polyclonal antibody responses and their epitopes is essential for countermeasure design. In this dissertation, I explore the epitopes and functional properties of monoclonal antibodies targeting the structural proteins of two families of RNA viruses, flaviviruses and coronaviruses.Flaviviruses are globally distributed arthropod-borne positive-sense RNA viruses transmitted primarily by either mosquitos or ticks. Symptomatic …

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The Effects Of Nmda Antagonism On Neuronal Activity And Neurovascular Coupling In Non-Human Primate Cortex, Benjamin Thomas Acland Dec 2022

The Effects Of Nmda Antagonism On Neuronal Activity And Neurovascular Coupling In Non-Human Primate Cortex, Benjamin Thomas Acland

Arts & Sciences Graduate Student Theses and Dissertations

The N-methyl-D-aspartate receptor (NMDAR) plays a variety of important roles in the development and function of primate central nervous systems. This thesis describes three nonhuman primate studies that, together, demonstrate that pharmacologically reducing NMDAR activity not only causes dramatic changes in neural activity in cortex, but also changes the relationship between that activity and a key signal often used as its proxy in human studies, the blood-oxygen-level-dependent (BOLD) signal. The first study reveals that NMDA antagonism is sufficient to induce powerful low-frequency modulation of spiking activity and extracellular local field potential (LFP) and discusses the implications of this finding for …

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Understanding Individual Cells In Zebrafish Caudal Fin Regeneration (从单细胞视角理解斑马鱼尾鳍再生历程), Yiran Hou Dec 2022

Understanding Individual Cells In Zebrafish Caudal Fin Regeneration (从单细胞视角理解斑马鱼尾鳍再生历程), Yiran Hou

Arts & Sciences Graduate Student Theses and Dissertations

Humans are incapable of complex body structure regeneration. Understanding how other species retain the ability to regenerate can help us to find solutions for improving our regenerative capacities. The zebrafish is an organism that is capable of regenerating multiple organs in its body. Using zebrafish as a model organism, previous studies have identified key processes and illustrated the crucial cell types and genes for regeneration. In caudal fin specifically, blastema formation is a key predictor of successful regeneration. However, a comprehensive molecular understanding of cell type-specific contributions to this process is lacking. In this dissertation, I present our efforts toward …

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Fructose Promotes The Growth Of Tumors Through Metabolite Exchange With Non-Malignant Tissues, Ronald Joseph Fowle-Grider Dec 2022

Fructose Promotes The Growth Of Tumors Through Metabolite Exchange With Non-Malignant Tissues, Ronald Joseph Fowle-Grider

Arts & Sciences Graduate Student Theses and Dissertations

The amount of dietary fructose consumed in the United States has increased considerably over the past five decades, largely due to the widespread use of high fructose corn syrup as a sweetener. The potential link between this elevated fructose intake and the development of cancer remains incompletely understood. Here we show that, even though cervical cancer cells themselves cannot directly utilize fructose carbon, diets high in fructose enhance the growth of cervical tumors by nearly threefold in mice. We demonstrate that efficient utilization of fructose carbon requires ketohexokinase-C, which was not expressed in ~100 cervical tumors that we examined from …

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