Life Sciences Commons^™

Early Detection, Rapid Response Invasives Management: Documenting Perennial Pepperweed In New Hampshire Using Eddmaps, Final Project Report, December 2022, Malin Clyde

UNH Cooperative Extension

No abstract provided.

The Impact Of Habitual Dietary Fat Intake On Postprandial Lipemia In Older Adults, Sophia A. Bollinger

Masters Theses, 2020-current

Postprandial lipemia (PPL) is a risk factor for cardiovascular disease (CVD). Many older Americans experience elevated PPL due to a chronically high-fat diet and an inefficient ability to clear triglycerides (TG) from circulation. TG levels are typically measured in a fasted state; however, postprandial TG levels may be more strongly associated with CVD risk. This study grouped ten older adults into two dietary consumption fat categories and examined the response to an acute high-fat meal by measuring postprandial blood cholesterol, TG, and glucose (GLU) at baseline, 30-minutes post-meal, 60 minutes, 90 minutes, 120 minutes, 180 minutes, and 240 minutes. Participants …

Relationships Among Age, Relative Telomere Length, And Dna Methylation In Pteropus Bats, Erika E. Forest

Masters Theses

Bats are the longest-lived mammalian order relative to body mass, but questions remain about relationships among mechanisms related to aging. Relative telomere length (rTL) decreases with age in most mammals, but the relationship between age and rTL is highly variable among the few bat taxa studied to date. More recently, DNA methylation (DNAm), a type of epigenetic regulation in which methyl groups are added to cytosine-phosphate-guanine (CpG) sites across the genome, has been shown to be highly predictive of age in many species of bats. We examine the relationships among age, rTL, and DNAm in Pteropus pumilus and P. hypomelanus …

Description Of A New Species Of Aleuroparadoxus Quaintance And Baker (Hemiptera: Aleyrodidae) From Guatemala, José Francisco García Ochaeta, Anil Kumar Dubey

Insecta Mundi

Puparia of a new whitefly, Aleuroparadoxus marisae García-Ochaeta and Dubey, new species (Hemiptera: Aleyrodidae), collected on leaves of Brosimum alicastrum Sw. in Santa Ana, Petén, Guatemala, and on Pimenta dioica (L). Merr. in Calzada Mopán, Dolores, Petén are described and illustrated. Drawings of morphological structures and differential diagnosis of the puparium are provided, and differences from congeners are discussed. A key to puparia of the Aleuroparadoxus Quaintance and Baker species is included.

Eavesdropping On Animals: Can Bioacoustics Help Save Species?, Zoe Grueskin

Capstones

Around the world, scientists are using sound to study the natural world in a growing field called bioacoustics. Researchers are eavesdropping on frogs and fish, elephants and earthworms, and many hope what they hear can inform and inspire conservation action around the world. From the field’s auspicious beginning with accidentally-recorded whales, to researchers today listening to locations as diverse as the Arctic seafloor and India’s Western Ghats mountain range, this capstone project explores the potential — and limitations — of conservation bioacoustics. Read the story, see photos and listen to audio pieces featuring three bioacousticians and their field recordings here: …

A Stealth Health Approach To Dietary Fibre, P. Stephen Baenziger, Katherine Anna Frels, Steve Greenspan, Julie Jones, Alison Lovegrove, Devin J. Rose, Peter Shewry, Rod Wallace

Department of Agronomy and Horticulture: Faculty Publications

Average dietary fibre intakes have increased little in the past twenty years in many countries, including the USA¹ . Multi-million-dollar campaigns promoting fruits, vegetables, whole grains and other foods high in fibre have delivered only small changes in diets² , and consumers have not changed from traditional staples to whole-grain options³ . UK millers report that consumption of whole-wheat bread has actually declined over the past decade (P. Shewry, personal communication). In the US, white flour, which is lower in fibre than whole-wheat flour, accounts for nearly 40% of the fibre intake⁴ . We believe that …

Hepatocyte-Hepatic Stellate Cell Axis In Potentiation Of Alcohol And Hiv-Induced Liver Injury, Moses O. New-Aaron

Theses & Dissertations

Human Immunodeficiency Virus (HIV) remains a global threat, with approximately 38.4 million active infections and 33 million HIV-related deaths since the first case. While many may be tempted to think of HIV as a relic of the past, emerging data suggests otherwise. By the end of 2021, approximately 1.5 million HIV incidences and 650 000 mortalities were reported globally. The etiologies of HIV-related mortality are numerous. Liver disease is one of the leading etiologies of HIV-related mortality, especially in recent times of antiretroviral therapy (ART)-induced longevity among people living with HIV (PLWH). While co-infections with hepatotropic viruses notoriously contribute to …

Structural And Biochemical Studies Of Three Enzymes Involved In Amino Acid Metabolism To Develop Novel Drugs, Yahani Pankaja Jayasinghe Arachchige

Theses & Dissertations

Intrinsic drug resistance in bacteria predates the clinical use of antibiotics. Additionally, heterogeneous metabolic states and the ability of many bacteria to form biofilms further enhances bacterial survival during infection and exacerbates treatment challenges. This necessitates the identification of novel drugs against new drug targets. These studies focus on the development of compounds targeting enzymes in Mycobacterium tuberculosis (Mtb) and Staphylococcus aureus that avoid intrinsic antibiotic defenses. The Mtb-encoded homoserine transacetylase enzyme (HTA) catalyzes the first step of the methionine biosynthesis pathway that produces methionine and S-adenosyl methionine. These compounds are shown to be essential for both dormant …

Isolation, Culture, And Immunophenotypic Analysis Of Bone Marrow Hspcs From Patients With Myelodysplastic Syndromes, Irene Ganan-Gomez, Karen Clise-Dwyer, Simona Colla

Faculty, Staff and Student Publications

Drug testing assays in hematopoietic stem and progenitor cells (HSPCs) are fundamental in biological studies of myelodysplastic syndromes (MDS) but have historically entailed a technical challenge. This protocol allows the efficient isolation of MDS HSPCs from bone marrow mononuclear cell fractions and their culturing with the support of stromal cells for improved maintenance during drug testing. Lastly, specific steps are given to quantify surviving cells and assess changes in the HSPC hierarchies.

For complete details on the use and execution of this protocol, please refer to Ganan-Gomez et al. (2022).

Processing And Cryopreservation Of Human Ureter Tissues For Single-Cell And Spatial Transcriptomics Assays, Emily E Fink, Surbhi Sona, Byron H Lee, Angela H Ting

Faculty, Staff and Student Publications

Characterizing the cellular heterogeneity of human ureter tissues using single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics provides a detailed atlas of cell types, signaling networks, and potential cell-cell cross talk underlying developmental and regenerative pathways. We describe an optimized protocol for generating, cryopreserving, and thawing single-cell suspensions from ureter tissues isolated post-cystectomy for scRNA-seq. In addition, we describe an optimized protocol for cryopreserving human ureter tissues for 10x Genomics Visium spatial gene expression platform. For complete details on the use and execution of this protocol, please refer to Fink et al. (2022).

Protocol For Mathematical Prediction Of Patient Response And Survival To Immune Checkpoint Inhibitor Immunotherapy, Joseph D Butner, Maguy Farhat, Vittorio Cristini, Caroline Chung, Zhihui Wang

Faculty, Staff and Student Publications

This protocol describes the application of a mechanistic mathematical model of immune checkpoint inhibitor (ICI) immunotherapy to patient tumor imaging data for predicting solid tumor response and patient survival under ICI intervention. We describe steps for data collection and processing, data pipelines, and approaches to increase precision. The protocol is highly predictive as early as the first restaging after treatment start and can be used with standard-of-care imaging measures. For complete details on the use and execution of this protocol, please refer to Butner et al. (2020)

Egfr Is A Master Switch Between Immunosuppressive And Immunoactive Tumor Microenvironment In Inflammatory Breast Cancer, Xiaoping Wang, Takashi Semba, Ganiraju C Manyam, Jing Wang, Shan Shao, Pascal Finetti, Lan Thi Hanh Phi, Troy Pearson, Steven J Van Laere, Jared K Burks, Evan N Cohen, James M Reuben, Fei Yang, Hu Min, Nicholas Navin, Van Ngu Trinh, Toshiaki Iwase, Harsh Batra, Yichao Shen, Xiang Zhang, Debu Tripathy, Naoto T Ueno

Faculty, Staff and Student Publications

Inflammatory breast cancer (IBC), the most aggressive breast cancer subtype, is driven by an immunosuppressive tumor microenvironment (TME). Current treatments for IBC have limited efficacy. In a clinical trial (NCT01036087), an anti-EGFR antibody combined with neoadjuvant chemotherapy produced the highest pathological complete response rate ever reported in patients with IBC having triple-negative receptor status. We determined the molecular and immunological mechanisms behind this superior clinical outcome. Using novel humanized IBC mouse models, we discovered that EGFR-targeted therapy remodels the IBC TME by increasing cytotoxic T cells and reducing immunosuppressive regulatory T cells and M2 macrophages. These changes were due to …

In Vivo Characterization Of Glutamine Metabolism Identifies Therapeutic Targets In Clear Cell Renal Cell Carcinoma, Akash K Kaushik, Amy Tarangelo, Lindsey K Boroughs, Mukundan Ragavan, Yuanyuan Zhang, Xiangyi Li, Jui-Chung Chiang, Vanina T Tcheuyap, Faeze Saatchi, Quyen N Do, Cissy Yong, Tracy Rosales, Christina Stevens, Aparna D Rao, Brandon Faubert, Panayotis Pachnis, Lauren G Zacharias, Hieu Vu, Feng Cai, Thomas P Mathews, Giannicola Genovese, Barbara S Slusher, Payal Kapur, Xiankai Sun, Matthew Merritt, James Brugarolas, Ralph J Deberardinis

Faculty, Staff and Student Publications

Targeting metabolic vulnerabilities has been proposed as a therapeutic strategy in renal cell carcinoma (RCC). Here, we analyzed the metabolism of patient-derived xenografts (tumorgrafts) from diverse subtypes of RCC. Tumorgrafts from VHL-mutant clear cell RCC (ccRCC) retained metabolic features of human ccRCC and engaged in oxidative and reductive glutamine metabolism. Genetic silencing of isocitrate dehydrogenase-1 or isocitrate dehydrogenase-2 impaired reductive labeling of tricarboxylic acid (TCA) cycle intermediates in vivo and suppressed growth of tumors generated from tumorgraft-derived cells. Glutaminase inhibition reduced the contribution of glutamine to the TCA cycle and resulted in modest suppression of tumorgraft growth. Infusions with …

Elevation Influences The Microhabitat Use And Baseline Physiology Of Coqui Frogs In Hawai‘I, Karen H. Beard, Susannah S. French, Jack Marchetti

Browse all Datasets

The purpose of this study was to determine whether elevation in HawaiÔi affects the coquiÕs microhabitat use such as substrate choice and height off the forest floor and physiological metrics such as osmolality, oxidative status, and energy metabolites (glucose, free glycerol, and triglycerides).

The Evolutionary Dynamics Of Infectious Diseases On An Unstable Planet: Insights From Modeling The Stockholm Paradigm, Angie T. C. Souza, Sabrina B. L. Araujo, Walter A. Boeger

MANTER: Journal of Parasite Biodiversity

Emerging infectious diseases (EIDs) are, besides a question of food safety and public health, an ecological and evolutionary issue. The recognition of this condition combined with the accumulation of evidence that pathogens are not specialists in their original hosts evidences the need for understanding how the dynamics of interaction between pathogens and hosts occurs. The Stockholm Paradigm (SP) provides the theoretical fundaments to understand the dynamics of diseases and design proactive measures to avoid the emergence and reemergence of infectious diseases. In this review, we revisit the models that evaluate several aspects of the proposed dynamics of the SP, including …

Studi Pendahuluan Regenerasi Eksplan Teh Sebagai Upaya Percepatan Penyediaan Bibit Unggul Secara In Vitro, Intan Ratna Dewi Anjarsari, Erni Suminar, Murgayanti

Jurnal Kultivasi

Pembiakan generatif tanaman teh yang dilakukan dengan biji, sementara secara vegetatif dengan setek tunas yang mempunyai kelemahan, antara lain jumlah bibit yang dihasilkan terbatas, perlu waktu lama untuk menyeleksi pohon induk, dan bibit yang dihasilkan kurang optimal dalam penyerapan air dan unsur hara karena perakarannya dangkal sehingga kurang toleran terhadap kekeringan. Kultur jaringan menjadi salah satu alternatif dalam perbanyakan teh memperoleh klon teh unggul, seperti klon Seri Gambung 1-11, dalam jumlah banyak dan sifatnya yang seragam. Penelitian pendahuluan ini dilaksanakan di Laboratorium Kultur Jaringan Departemen Budidaya Pertanian Fakultas Pertanian UNPAD, mulai bulan Juni hingga Desember 2021. Penelitian terdiri dari dua …

Resistensi Gulma Echinochloa Crusss-Galli Terhadap Herbisida Berbahan Aktif Metamifop Di Areal Persawahan Sulawesi Selatan, Annisa Nadiah Aprilia, Denny Kurniadie, Uum Umiyati

Jurnal Kultivasi

Barnyardgrass [Echinochloa cruss-galli (L.) P. Beauv.] diakui sebagai gulma paling bermasalah di areal persawahan Provinsi Sulawesi Selatan, Indonesia. Herbisida metamifop sudah lama digunakan untuk mengendalikan gulma tersebut pada areal pertanaman padi sawah di Sulawesi Selatan dengan intensitas aplikasi yang cukup tinggi. Hal tersebut menimbulkan E. cruss-galli yang sulit dikendalikan dan diperkirakan resisten terhadap herbisida metamifop. Namun demikian, kasus resistensi gulma terhadap herbisida metamifop di Indonesia belum banyak dilaporkan dan diteliti. Penelitian ini bertujuan untuk mengetahui (1) adanya resistensi gulma E. cruss-galli terhadap metamifop, (2) tingkat resistensi yang terjadi pada gulma E. cruss-galli terhadap metamifop. Penelitian dilakukan di rumah kaca Fakultas …

The Y-Chromosome's Role In Sexually Dimorphic Evolution And Mother's Curse Compensation, Tobias Nielsen

Electronic Theses and Dissertations, 2020-2023

The Y-chromosome and mitochondria (containing mtDNA) appear entirely dissimilar, the former being a sex chromosome found in the nucleus and the latter an organelle with thousands of copies in the cytoplasm. However, both experience uniparental inheritance, with Y-chromosomes being patrilineal and mitochondria matrilineal. This leads to various other similarities including haploidy, high mutation rates, and limited or lack of genetic recombination. Moreover, the low number of protein-coding genes contained on both led to the belief that neither contributes significantly to the adaptive evolution of complex traits. However, recent studies show the Y-chromosome and mitochondria both influence roughly 10% of male …

Insights Into The Biotechnology Potential Of Methanosarcina, Sean Carr, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Methanogens are anaerobic archaea which conserve energy by producing methane. Found in nearly every anaerobic environment on earth, methanogens serve important roles in ecology as key organisms of the global carbon cycle, and in industry as a source of renewable biofuels. Environmentally, methanogenic archaea play an essential role in the reintroducing unavailable carbon to the carbon cycle by anaerobically converting low-energy, terminal metabolic degradation products such as one and two-carbon molecules into methane which then returns to the aerobic portion of the carbon cycle. In industry, methanogens are commonly used as an inexpensive source of renewable biofuels as well as …

Understanding The Ahupua'a: Using Remote Sensing To Measure Upland Erosion And Evaluate Coral Reef Structure, Logan Kalaiwaipono Ellis

Theses and Dissertations

Under ever intensifying pressures from land use, climate change, and erosion, tropical islands are among the most vulnerable systems in the world. Terrestrial systems are weakened by intensifying land use patterns, the weakening of which is highlighted when high intensity rainfall events erode sediment and leads to sediment deposition on the marine system. The deposition of sediment on the marine system is a major stressor that can lead to weakened coral reefs and a decrease in marine resources commonly gathered for food. These interactions have led to the emergence of biocultural resource management strategies, one of which is the ahupua'a …

An Amino-Terminal Fragment Of Apolipoprotein E4 Leads To Behavioral Deficits, Increased Phf-1 Immunoreactivity, And Mortality In Zebrafish, Madyson M. Mccarthy, Makenna J. Hardy, Saylor E. Leising, Alex M. Lafollette, Erica S. Stewart, Amelia S. Cogan, Tanya Sanghal, Katie Matteo, Jonathon C. Reeck, Julia T. Oxford, Troy T. Rohn

Biology Faculty Publications and Presentations

Although the increased risk of developing sporadic Alzheimer’s disease (AD) associated with the inheritance of the apolipoprotein E4 (APOE4) allele is well characterized, the molecular underpinnings of how ApoE4 imparts risk remains unknown. Enhanced proteolysis of the ApoE4 protein with a toxic-gain of function has been suggested and a 17 kDa amino-terminal ApoE4 fragment (nApoE4_1-151) has been identified in post-mortem human AD frontal cortex sections. Recently, we demonstrated in vitro, exogenous treatment of nApoE4_1-151 in BV2 microglial cells leads to uptake, trafficking to the nucleus and increased expression of genes associated with cell toxicity …

Antioxidant And Α-Amylase Inhibitory Activities And Starch Hydrolysis Kinetics Of Kidney Bean By Simulating Digestion In Vitro, Shi Juan, Bao Hong-Hui, Zhang Pei, Zhang Ti-Pei, Zhou Rui

Food and Machinery

Objective: To evaluate the physiological effects of whole kidney been food and develop functional resources of different color kidney beans. Methods: The contents of total polyphenols, flavonoids and acid polysaccharides, and antioxidant and α-amylase inhibitory activities of digestive solution and starch hydrolysis kinetics and glucose diffusion properties of three kidney bean were investigated through in vitro simulated gastrointestinal digestion. Results: The contents of total polyphenols, flavonoids and acid polysaccharides, and antioxidant and α-amylase inhibitory activities were black kidney bean＞red kidney bean＞white kidney bean (P＜0.05). The kinetic rate of first order reaction of starch hydrolysis, rapidly digestible starch …

Research Progress On Extraction Of Phenols With Natural Deep Eutectic Solvent, Zhang Yi-Xin, Wu Xu-Ran, He Ruo-Han, Feng Shuo, Yang Feng-Qi

Food and Machinery

This review summarized the research status of NADES on the extraction and separation of phenols, discussed the main factors affecting the extraction efficiency, and prospected the future development direction.

Extracellular Vesicle-Associated Biomolecules As Potential Biomarkers For Alzheimer's Disease Diagnosis, Lina Bedoya Martinez

Electronic Theses and Dissertations, 2020-2023

Alzheimer's disease (AD) involves progressive neurodegeneration leading to the loss of normal neuronal function. Extracellular accumulation of amyloid-beta (Aß), through the abnormal cleavage of the amyloid precursor protein (APP) by ß- and γ-secretases, is one of the hallmarks of AD. Current research focuses on finding potential candidates for biomarkers and techniques with improved sensitivity for early disease detection. Extracellular vesicles (EVs) found in body fluids are a source of biomarkers for AD diagnosis. EVs transport pathologically significant biomolecules, like nucleic acids and proteins, across the blood-brain barrier, mediating local and distant cell-to-cell communication. Therefore, this study evaluated EV-associated DNA and …

Étude Visant À Mieux Guider L’Évaluation Des Risques Des Travailleurs Exposés Aux Nanoparticules (Np) : Existe-T-Il Différentes Propriétés Inflammatoires Des Np Liées Au Sexe?, Marion Vanharen, Abdelaziz Saafane, Alexanne Léveillé, Thomas Mahbeer, Denis Girard

Rapports de recherche scientifique

Les nanotechnologies offrent un important potentiel d’exploitation dans des secteurs industriels variés, pour lequel les bénéfices financiers ne sont pas négligeables. À l’instar des provinces canadiennes et de nombreux pays industrialisés, le Québec voit un nombre grandissant de compagnies produisant des nanomatériaux et des nanoparticules (NP) se développer, sans oublier celles qui introduisent de plus en plus de NP dans leurs produits. Le nombre de travailleurs québécois œuvrant dans la fabrication et dans la synthèse des NP augmentera dans les années à venir. De plus, le nombre de travailleurs appelés à manipuler et à transformer des NP dans les domaines …

Of Bugs And Wildfires: Tracing The Impacts Of Changing Wildfire Regimes On Aquatic Bacteria And Macroinvertebrates Using Edna, Isabella M. Errigo

Theses and Dissertations

Human disruption of climate, habitat, and ignition has altered the behavior of wildland fire at local to continental scales. In many regions, novel fire regimes are emerging that threaten to exceed the capacity for local management to protect human wellbeing and ecosystem function. Simultaneous changes in climate, species composition, and fire management have resulted in extreme fire behavior in many regions. For the Western United States, the emerging novel fire regime consists of more frequent, severe, and intense wildfires, with annual area burned by wildfire having doubled and high-severity wildfire area having increased 8-fold since the 1980s. The impacts of …

Research Progress On Properties, Industrial Production Of Reduced Glutathione And Its Application In Big Health Industry, Cai You-Hua, Wu Zi-Hao, Huang Xiao-Chen, Lin Yu-Kuan, Zheng Ming-Ying

Food and Machinery

The structural characteristics and physiological activities of reduced glutathione (GSH), as well as its metabolic pathways in vivo were reviewed, and the research progress in preparation, extraction, purification, application and industrial production of GSH were summarized. Moreover, the future research directions in functional activity, industrial production and product application of GSH were prospected.

Study On Nutrition Labeling Optimization Based On Augmented Reality, Huang Ze-Ying, Li Hai-Jun

Food and Machinery

To optimize nutrition labeling in China, this paper tried to overlay virtual information on original information from the nutrition information table by using augmented reality (AR), and then added three new guidance functions of nutrition labeling knowledge popularization, nutritional content auxiliary calculation and precise nutrition guidance through database construction, 3D modeling building and mobile phone application making. For the implementation of AR nutrition labeling, some suggestions were put forward, such as the establishment of national nutrition health database, the forecast of the implementation effect of the labeling and the operation mechanism design.

Analysis And Comprehensive Evaluation Of Amino Acid Composition Of Coreopsis Tinctoria From Different Habitats, Lu Qing-Yao, Zhou Min, Zhou Hui-Ling, Jiao Shi-Rong

Food and Machinery

Objective: This study focuses on in-depth research and development of the utilization of Coreopsis tinctoria resources. Methods: The Coreopsis samples from Hetian, Urumqi, Aba, Luding and Xining were hydrolyzed with hydrochloric acid, and the contents of 17 kinds of amino acids were determined by automatic amino acid analyzer. On this basis, the characteristics of amino acid flavor were analyzed and compared with the amino acid pattern spectrum issued by FAO/WHO. RAA, RC and SRC were calculated, and correlation analysis and cluster analysis were performed on 17 kinds of amino acids of chrysanthemum. Thereafter, the nutritional value of Coreopsis …

Correlation Analysis Of Taste Intensity Of Tea Flavor Attribute Reference Based On Electronic Tongue And Artificial Senses, Liu Zhong-Ying, Ran Qian-Song, Zhang Tuo, Zheng Wen-Jia, Pan Ke

Food and Machinery

Objective: This study aimed to explore the correlation between electronic tongue and artificial senses on the taste intensity of tea taste attribute reference. Methods: Quinine, alum, sodium glutamate, sucrose and citric acid were used as the reference materials for bitter, astringent, fresh, sweet and sour taste attributes in turn. Based on the perception threshold, the relationship between concentration and taste intensity of each reference material for electronic tongue and artificial senses and its correlation were analyzed. Results: The bitterness perception threshold of quinine was 0.015 mg/mL, and the corresponding electronic tongue response value was 4.91. The detection …