Life Sciences Commons^™

Characterizing The Effects Of Antiandrogens And Senolytics To Enhance The Therapeutic Response To Castration-Resistant Prostate Cancer, Justin M. Silverman

Theses and Dissertations

Prostate cancer is the most frequently diagnosed cancer in males and the second most common cause of cancer deaths. Androgen deprivation therapy, whether through surgical or chemical castration, is the mainstay for treatment of advanced prostate cancer; however, despite an initial response, most patients eventually develop a progressive PSA rise, and castration- sensitive prostate cancer gives rise to castration-resistant prostate cancer. The standard of care therapy includes the antiandrogens such as enzalutamide and abiraterone acetate as well as the microtubule poison, docetaxel, and various immunotherapies; however, while prostate cancer research is progressing, there continues to be a compelling need for …

Stage-Structured Predator-Prey Interactions In A Warming World: A Case Study In Riverine Rock Pools, Andrew T. Davidson

Theses and Dissertations

Warming can impact predator-prey interactions through a variety of mechanisms. For example, warming can both alter the rate at which predators consume prey and the rate prey develop through vulnerable life stages. Thus, the overall effect of warming on consumer-resource interactions will depend upon the strength and asymmetry of warming effects on predator and prey performance. Here, we describe the consequences of these asymmetrical responses to temperature by first developing a simple stage-structured modeling framework, then applying that framework to predation of American rock pool mosquito larvae, Aedes atropalpus, by several common predators in riverine rock pools. We then …

Integrative Post-Gwas Analyses Of Psychiatric Disorders: Identifying Putative Risk Genes And Gene Sets Using Transcriptome, Proteome And Methylome Information, Huseyin Gedik

Theses and Dissertations

Genome-wide association studies (GWAS) of psychiatric disorders (PD) yield numerous loci with significant signals, but often they do not implicate specific protein coding genes. Because GWAS risk loci are enriched in expression/protein/methylation quantitative loci (e/p/mQTL, hereafter xQTL), transcriptome/proteome/methylome-wide association studies (T/P/MWAS, hereafter XWAS), which integrate information from GWAS and x-level (mRNA, protein or DNA methylation levels) coming from largest xQTL studies, can link GWAS signals to effects on specific genes. For gene level analyses, researchers use mendelian randomization (MR) methods to fine-map the association between x-levels and trait. However, none of the previous studies ever jointly analyzed XWAS of multiple …

Association Of Interpersonal Trauma And Polygenic Risk Scores With Depressive Symptoms In College Students, Rowan K. O'Hara

Theses and Dissertations

Major depression is considered a complex trait influenced by both polygenic risk factors and environmental exposures, such as childhood trauma. This study applied statistical genetic methods to calculate aggregate genetic risk for major depression to predict depressive symptoms scores in a college student sample. Data were from the Spit for Science (S4S) study in which college students from a large urban university self-reported interpersonal trauma (IPT) exposure prior to college and depressive symptoms from the past month (N = 7502; ancestry group: 20% African [AFR], 12% Admixed Americas [AMR], 10% East Asian [EAS], 49% European [EUR], 8% South Asian [SAS]). …

The Impact Of Hiv-1 Tat And Morphine On Spatial Distribution Of Drugs In The Brain., Austin M. Jones

Theses and Dissertations

Despite combination antiretroviral therapy effectively suppressing HIV within the periphery, the central nervous system (CNS) remains affected by the virus. Approximately half of people living with HIV will experience HIV-associated neurocognitive disorders (HAND) or neuroHIV. Concurrent opioid use exacerbates neuroHIV by promoting neuroinflammation, viral replication, and potentially altering the antiretroviral concentrations within the brain.

Using a transgenic mouse that expresses the HIV-1 Tat protein, we examined the effects of Tat and morphine on antiretroviral accumulation and distribution and the effects of Tat on morphine accumulation within the brain using infrared matrix-assisted laser desorption electrospray ionization with mass spectrometry imaging (IR-MALDESI-MSI). …

Protacs – A Novel And Rapidly Developing Field Of Targeted Protein Degradation, Hannah R. Gatley

Theses and Dissertations

There is a continued need for new technology and strategies for tackling cancer and other diseases, and within the current century a novel therapeutic strategy has emerged in the realm of targeted protein degradation called Proteolysis-Targeting Chimeras (PROTACs). This technology specifically targets and degrades disease-causing proteins via the ubiquitin-proteasome system, and has seen an explosion of research and intrigue in both academia and industry over the past two decades. The diversity of PROTAC classes based on the E3 ligase recruiting ligand and the target protein allows for a universal molecular structure that can be customized for a specific target and …

Genomic Comparison Of Dba/2j And C57bl/6j Strains Of Mus Musculus And Best Practice Of Genome Alignment For Bioinformatics Analyses, Dustin J. Zeliff

Theses and Dissertations

Alcohol use disorder is known to have significant genetic components that contribute to an individual’s susceptibility to the disease. Mouse models are commonly used to study the mechanisms underlying alcohol use disorder, with C57BL/6J (B6) and DBA/2J (D2) being two of the more prominently used inbred strains. Research in the Miles Laboratory has used these two strains, and genetic panels of mice derived from them, to identify potential genes associated with variance in ethanol-related behaviors using quantitative trait loci (QTL) analysis. For example, Ninein (Nin) was identified as a potential candidate gene for the anxiolytic effects of ethanol, discovered because …

Novel Strategies In Head And Neck Tumor Therapy; Contribution Of Senescence In Therapy Response, And Resistance Development After Cisplatin Treatment, Fereshteh Ahmadinejad

Theses and Dissertations

Therapeutic outcomes achieved in head and neck squamous cell carcinoma (HNSCC) patients by concurrent cisplatin-based chemoradiotherapy initially reflect both tumor regression and tumor stasis. However, local, and distant metastasis and disease relapse as well as cisplatin-induced resistance are common in HNSCC patients. In the current work, we demonstrate that cisplatin treatment induces senescence in both p53 wild- type HN30 and p53 mutant HN12 head and neck cancer models. We also show that tumor cells can escape from senescence both in vitro and in vivo. We further established a cisplatin-resistant cell line from HN30 parental cell line that underwent brief senescence …

A Standardized Pipeline For Isolation And Assembly Of Genomes From Symbiotic Bacteria In Whole Louse Genomic Sequence Data., Mohammad Mikail I. Bala

Theses and Dissertations

Many insects are known to harbour intracellular and heritable bacteria (endosymbionts), which provide their hosts with adaptive traits. Whole insect gDNA shotgun sequencing projects often sequence the genome of endosymbiont, in addition to the insect’s genome. There are approximately 600 whole genome shotgun libraries from insects available on the public repository (NCBI), which can be mined to obtain endosymbiont genomes. The assembly and annotation of endosymbiont genomes can contribute towards the exploration of their role as obligate symbiotic partners. However, de novo assembly of an endosymbiont genome, continues to be challenging, when the host and/or enteric bacterial gDNA is present …

Identification Of Proteins Involved In Cell Membrane Permeabilization By Nanosecond Electric Pulses (Nsep), Giedre Silkuniene, Uma Mangalanathan, Alessandra Rossi, Peter A. Mollica, Andrei G. Pakhomov, Olga N. Pakhomova

Bioelectrics Publications

The study was aimed at identifying endogenous proteins which assist or impede the permeabilized state in the cell membrane disrupted by nsEP (20 or 40 pulses, 300 ns width, 7 kV/cm). We employed a LentiArray CRISPR library to generate knockouts (KOs) of 316 genes encoding for membrane proteins in U937 human monocytes stably expressing Cas9 nuclease. The extent of membrane permeabilization by nsEP was measured by the uptake of Yo-Pro-1 (YP) dye and compared to sham-exposed KOs and control cells transduced with a non-targeting (scrambled) gRNA. Only two KOs, for SCNN1A and CLCA1 genes, showed a statistically significant reduction in …

A New Annual Satureja (Lamiaceae) Species From Turkey With Molecular Evidence, And Lectotypification Of Two Species, Hayri̇ Duman, Tuncay Di̇rmenci̇, Taner Özcan

Turkish Journal of Botany

A new species, Satureja hasturkii, is described using morphological characters and molecular studies in this study. The new species is closely related to S. hortensis but differs from it by very lax thyrsoid inflorescence, longer pedicels, shorter calyx, upper and lower calyx teeth, and corolla. In order to support the morphological data, molecular studies are also done using nrITS and cptrnL-F gene regions. nrITS data gives better results in phylogenetic analysis. It is pointed out that the closest relatives of S. hasturkii are S. hortensis and S. laxiflora. The fact that having annual life forms of …

Drought Stress Effects On Morphophysiological And Quality Characteristics Of Commercial Carrot Cultivars, Muhammad Daniyal Junaid, Zahi̇de Nesli̇han Öztürk, Ali̇ Fuat Gökçe

Turkish Journal of Botany

The effects of drought stress on plant growth and development are getting more pronounced due to increasing influence of climate change on environmental stresses. Current study was devised to explore the drought effects on eight commercial carrot cultivars, having different root colors as a base for further studies to understand the response of carrot. Drought stress for 10 days was applied to carrot plants at taproot formation stage in semicontrolled greenhouse. The results revealed that orange and yellow colored carrot cultivars exhibited the least decline in physiological functioning (relative water contents and dry matter) that assisted in maintaining higher yield …

Secondary Traumatic Stress Is An Occupational Hazard For Pet Hospice Support Staff: Psychoeducational Support Groups Provide The Cure, Kari Hayden

DSW Capstone Projects

There are three separate yet interrelated products presented in this final capstone product. The completed product is focused on pet hospice veterinary support staff and the need for an evidence-based social work intervention to combat secondary traumatic stress. This capstone uses the andragogical approach to adult learning theory as the foundation to construct a psychoeducational peer support group specific to this population. Implications for clinical social work in veterinary medicine are presented within this project.

The first scholarly product presented is the Systematic Literature Review (SLR). The SLR utilizes a scoping review process in an attempt to locate prior research …

An Exploration Of The Causes And Effects Of Interpersonal Conflict Related Stress Among A Self-Selected Cohort Of Second Level Teachers In Ireland, Patrick Bruce

Theses

Teaching has been classified by researchers as a high stress profession. Stress in teaching has been reported to have many negative health outcomes and also affects productivity. The literature also reports that stress in teaching is a difficult matter to resolve satisfactorily, with mixed outcomes from interventions. An emergent cause of work-related stress in education is IPC and this study sets out to investigate the prevalence of this underreported phenomena.

A sample of 25 teachers working in Irish second level schools were recruited. Semi structured interviews were conducted to report on the experiences of stress, it’s causes and effects both …

The Impact Of Wheat Resistance And Bio-Rational Insecticides Toxicity Against Cherry-Oat Aphid, Rhopalosiphum Padi L. (Hemiptera: Aphididae), Anisa Haq, Waheed Murad, Sarir Ahmad

Turkish Journal of Zoology

Aphids cause substantial damage to the wheat crop. In order to determine how host plant resistance and insecticides manage the Rhopalosiphum padi L. Current experiments were carried out to find the influence of host plant resistance and bio-rational insecticides against aphids in wheat (Triticum aestivum L.) crop. Field experiments were carried out for two growing years (2017/18 and 2018/19) to access the resistance of wheat against the aphids. The field resistance of wheat is highly expressed in Pirsabak-08 variety (infestation range = 1-2.5) while the most susceptible variety is Faisalabad-08 (infestation range > 10). The varietal preference experiment results revealed …

Ecological Factors Influencing The Breeding Performance Of Great Tits (Parus Major) In Artificial Nest Boxes, Dong-Ho Lee, Jae-Kang Lee, Tae-Kyung Eom, Ho-Kyoung Bae, Hyeongyu Ko, Shin-Jae Rhim

Turkish Journal of Zoology

Installing an artificial nest box has a positive effect on the cavity nesters such as great tit (Parus major). Beyond the process of installing an artificial nest box, a study on the effect of ecological factors on breeding performance is necessary. This study was conducted to evaluate the ecological factors influencing breeding performance of great tits (Parus major) in artificial nest boxes in temperate mixed forests. In this study, the first egg-laying date and percentage of shrub were closely related among ecological factors. Variable influencing clutch size was date of first egg-laying date. Percentage of shrub …

The Red-Footed Falcon Falco Vespertinus Population In The Danube Delta And Its Habitat Selection For Breeding, Lucian-Eugen Bolboaca, Alexandru-Catalin Dorosencu, Mihai Marinov, Marta Peraita, Maxym Yakovliev, Vasile Alexe

Turkish Journal of Zoology

The Danube Delta is an atypical and complex ecosystem on the border of the slowly shrinking breeding distribution of Falco vespertinus. In this paper, we study the population that breeds in this area and intend to understand its relationship with the environment in these circumstances. We registered 231 breeding pairs (bp), with a local density of 0.034 bp/km$^{2}$. We made a Nearest Neighbour Analysis which confirmed the natural condition of the facultative colonial nesting character of this species. In addition, in order to study its habitat preferences for nesting we did a binary logistic regression model and complemented its …

Redescription Of A Little-Known Species, Cryptocephalus Surdus Rapilly, 1980 (Coleoptera: Chrysomelidae: Cryptocephalinae), With Notes On New Distribution Localities And Habitat Preference In Türkiye, Ali̇ Gök, Abdullah Hasbenli̇, İsmai̇l Şen

Turkish Journal of Zoology

Cryptocephalus surdus Rapilly, 1980 is a little-known species which was described based on specimens collected from Iran. Due to the fact that the species was described very briefly in its original description, the taxonomic features of the species are redescribed in here. In addition, information about additional taxonomic characters, habitats and distribution in Türkiye of this little-known species are given for the first time in this study. Habitus and genital features of the species are photographed. Habitat preference and a map showing the previous and new distribution localities of the species are provided.

Diversity Rhythm In Pontellid Copepods (Pontellidae: Copepoda) From The Covelong Coast Pre- And Post-Covid-19 Lockdown, Bay Of Bengal, Md Anwar Nawaz, Sivakumar Kandhasamy, Baskar Gurunathan, Vijayaraj Radha

Turkish Journal of Zoology

Copepods act as indicators of the aquatic ecosystem since they rapidly respond to changes in nutrient content of the environment. Plankton samples were collected for two years from the Covelong coast, India (January to December 2019 and January to December 2021). The diversity patterns of pontellid copepods before and after the COVID-19 lockdown were analyzed. Physicochemical parameters like temperature, salinity, pH, dissolved oxygen, calcium, magnesium, nitrite, phosphate, and ammonia level for both years were measured to compare and contrast the coastal health before and after the lockdown. Six species of pontellid copepods were reported before the lockdown period and 10 …

Distribution And Population Density Of Halyomorpha Halys (Stål, 1855) (Hemiptera: Pentatomidae) In Black Sea Region Of Türkiye, Ki̇bar Ak, Mansur Uluca, Celal Tunçer

Turkish Journal of Zoology

Brown marmorated stink bug (BMSB), Halyomorpha halys, is a polyphagous invasive pest whose spread and damage are increasing with global commercial activities. In this study, following the invasion of Black Sea Region in 2018, spread, population density and change of the pest were investigated in the years 2019, 2020, and 2021, possible causes of spread and measures to be taken were evaluated. Course of population was monitored with pheromone traps placed in 141, 120, and 162 locations in 8 provinces along the coastline of Black Sea Region. High population density was detected at all locations of Artvin and Rize …

Molecular Phylogenetics Of Aedes Aegypti (L., 1762) (Diptera: Culicidae) In Eastern Black Sea Area Of Turkey And Possible Relations With The Caucasian Invasion, Murat Öztürk, Muhammet Mustafa Akiner

Turkish Journal of Zoology

Aedes aegypti (L., 1762) (Diptera: Culicidae) is an important pest for human health. It vectors many diseases, including yellow fever (YF), dengue (DENV), and chikungunya (CHIKV). This species has invaded numerous countries including Balkan and Caucasian countries few past decades. The established populations of species were found in Turkey in 2015. We investigated the genetic variation, molecular phylogenetics, and differences between samples from Turkey and Georgia using four mitochondrial and one genomic DNA markers. The possible origin of the species was determined separately for each gene region using maximum likelihood trees. The ML analysis showed a close relation with the …

The Importance Of Shape Analysis Of The First Upper Molar In The Separation Of Two Subspecies Of The Hazel Dormouse (Muscardinus Avellanarius (Linnaeus, 1758)) In Northern Anatolia, Güli̇z Yavuz, Hati̇ce Mutlu Eyi̇son, Erkut Kivanç, Engi̇n Selvi̇, Nuri̇ Yi̇ği̇t, Ercüment Çolak

Turkish Journal of Zoology

Morphological features are important for intraspecific and interspecific variation. Teeth are important taxonomical characteristics because they can differ according to diet. Shape analysis of the first upper molar (M1) was used to determine geographical variations and effects of ecological changes on the population structure of Hazel dormice (Muscardinus avellanarius) living in Turkey. Both outline and landmark analysis, as well as a canonical variates analysis, showed significant differences in teeth shape between populations. With this technique, we separated out two subspecies: M. a. trapezius and M. a. abanticus.

8th International Galliformes Symposium (East Java, Indonesia: October 9-13, 2023): Book Of Abstracts, Iucn-Ssc Galliformes Specialist Group, World Pheasant Association, Geoffrey Davison

Galliformes Specialist Group and Affiliated Societies: Conference Proceedings

Overview of the research and conservation of Galliformes in Indonesia, Jon-Paul Rodriguez

Overview of the research and conservation of Galliformes in Indonesia, Dewi M. Prawiradilaga

Impact of climate change and human pressure on Galliformes, Nurul L. Winarni and Habiburrachman A. H. Fuad

Distribution model of the great argus pheasant in the Bukit Barisan Selatan National Park, Femei Rahmilija, Jarwadi Budi Hernowo, and Lilik Budi Prasetyo

Surviving logging: The persistence of pheasants in sustainably managed logged forests in east Kalimantan, Indonesia, Agus Sudibyo Jati, Hiromitsu Samejima, and Kanehiro Kitayama

Respect for nature, respect for people: successful community-based conservation could lead to …

Salinization Impacts On Heterotrophic Respiration, Ecosystem Respiration, And Plant Productivity In Tidal Freshwater Marshes, Kelsie J. Moses

Theses and Dissertations

Tidal freshwater marshes have preserved substantial stocks of soil carbon, which represents carbon dioxide that is no longer in the atmosphere. There is conflicting evidence in the literature about how disturbances such as sea level rise and associated wetland salinization impact the accumulation and long-term stability of this stored carbon. The goal of this experiment was to quantify how salinization impacts total ecosystem respiration and its component parts, autotrophic and heterotrophic respiration. This was a microcosm experiment using a C4 plant (Spartina cynosuroides) grown in soil derived from C3 plant matter and exposed to different salinities (0 to …

Examining Genetically-Informed Etiologic Models Of Co-Occurring Posttraumatic Stress Disorder And Recreational Cannabis Use Among College Students, Terrell A. Hicks

Theses and Dissertations

The college years encompass a period of increased risk recreational cannabis use (RCU), as well as a time of increased risk for trauma exposure and developing posttraumatic stress disorder (PTSD). Given the high co-occurrence between RCU and PTSD, and the potentially negative consequences of the two (e.g., worse academic outcomes), there is a need to understand the etiologic mechanisms of these commonly co-occurring conditions. Two primary phenotypic models exist: self-medication model (i.e., PTSD to RCU) and the high-risk model (i.e., RCU to PTSD). To date, there are two existing studies longitudinally examining the etiologic models proposed to explain co-occurring RCU …

Population Demography Of A Neotropical Migratory Songbird In The Face Of A Changing Climate, Adele Balmer

Theses and Dissertations

This dissertation investigates the population dynamics and demographic trends of the Prothonotary Warbler (Protonotaria citrea), a migratory songbird that breeds in North America during the spring and summer and overwinters in Central and northern South America. The primary objective of this dissertation is to examine the combined implications of variation in weather and individual traits on demographic traits and to project the effects of climate change on population trajectories of the Prothonotary Warbler.

The analyses conducted in this dissertation centered around female Prothonotary Warblers; however, visual identification of a nestling’s sex is impossible in most altricial bird species, …

Engineering Ph-Dependent Antibody Interactions Through Introduction Of Linked Protonation Equibria Within The Antigen Binding Interface Or Linked Oligomeric Interfaces, Tosha Laughlin

Graduate Research Theses & Dissertations

Antibodies are widely applied as affinity reagents across a variety of fields, due to their high affinity and specificity. While high affinity across a range of conditions is useful for many applications, the ability to modulate affinity with environmental conditions (e.g., pH) opens new opportunities for antibody applications. This dissertation focuses on exploring the impact of an antibody’s interface size on engineered pH dependence, as well as exploring engineered pH-dependence through perturbing oligomerization equilibria that are linked to target binding. An anti-maltose binding protein (MBP) Fab 7O antibody fragment was used as a large protein-protein interface model to explore a …

Exploring Beaver-Facilitated Restoration In Grassland Channelized Streams, Jessica L. Sikora

Graduate Research Theses & Dissertations

Freshwater streams provide vital ecosystem services, but their ecological function is gravely impacted by agricultural practices especially in grassland ecosystems. Though there is evidence that the re-introduction of beavers can help restore the ecological function of streams, little is known about their restorative impacts in grasslands. At Nachusa Grasslands Preserve in Franklin Grove, IL, we investigated the presence and impact of beaver dams on their waterways. With increasing beaver dam volume, we found an increase in sediment retention and less sediment retention with increasing beaver dams upstream from a dam. Though we did not find evidence that beaver dam volume …

Effects Of Weight Bias Internalization On Disordered Eating, Dieting, And Intuitive Eating In College Students, Stacia Tietje

Graduate Research Theses & Dissertations

Weight bias internalization (WBI) is the tendency to blame oneself for negative weight-based stereotypes and external influences. WBI has been shown to have negative effects on both physical and mental health. The purpose of this study is to examine the association between WBI, disordered eating, and dieting and whether intuitive eating moderates these relationships in a sample of college students. This study utilized a cross-sectional survey design to gather quantitative data. College undergraduate students (N = 652) from a Midwestern university were recruited via email. Data were analyzed in SPSS, using descriptive statistics, multiple linear regression, ordinal, and binary logistic …

Homeobox Transcription Factor Hbxa Influences Expression Of Over One Thousand Genes In The Model Fungus Aspergillus Nidulans, Sandesh Sharma Pandit

Graduate Research Theses & Dissertations

In fungi, conserved homeobox-domain (HD) proteins are transcriptional regulators governing development. In Aspergillus species, several HD transcription factor genes have been identified, among them, hbxA/hbx1. For instance, in the opportunistic human pathogen Aspergillus fumigatus, hbxA is involved in conidial production and germination, as well as virulence and secondary metabolism (SM), including production of fumigaclavines, fumiquinazolines, and chaetominine. In the agriculturally important fungus Aspergillus flavus, disruption of hbx1 results in fluffy aconidial colonies unable to produce sclerotia. hbx1 also regulates production of aflatoxins, cyclopiazonic acid and aflatrem. Furthermore, transcriptome studies revealed that hbx1 has a broad effect on the A. flavus …