Life Sciences Commons^™

Validation Of An Emg Submaximal Method To Calibrate A Novel Dynamic Emg-Driven Musculoskeletal Model Of The Trunk: Effects On Model Estimates, Amir H. Eskandari, Farshid Ghezelbash, Aboulfazl Shirazi-Adl, Denis Gagnon, Hakim Mecheri, Christian Larivière

Études primaires

Background

Multijoint EMG-assisted optimization models are reliable tools to predict muscle forces as they account for inter- and intra-individual variations in activation. However, the conventional method of normalizing EMG signals using maximum voluntary contractions (MVCs) is problematic and introduces major limitations. The sub-maximal voluntary contraction (SVC) approaches have been proposed as a remedy, but their performance against the MVC approach needs further validation particularly during dynamic tasks.

Methods

To compare model outcomes between MVC and SVC approaches, nineteen healthy subjects performed a dynamic lifting task with two loading conditions.

Results

Results demonstrated that these two approaches produced highly correlated results …

Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry Quantitation Of O-Cresol In Hydrolyzed Human Urine To Assess Toluene Exposure, Eric Langlois, Sébastien Gagné

Études primaires

Rationale

Toluene is a volatile organic compound used in domestic and industrial applications. The main routes of workplace exposure to toluene are inhalation and dermal contact. As toluene exposure can cause severe nervous system damage, its quantification is crucial to prevent occupational illness. Toluene is metabolized mainly as hippuric acid, S-benzylmercapturic acid and epoxides. These are rapidly converted to o-/p-cresol, which is then excreted in the urine as conjugated glucuronides and sulfates. o-Cresol and its conjugates can be chemically hydrolyzed to form free o-cresol, which can then serve as a urinary biomarker of toluene exposure. Current …

Biology, Genetic Diversity, And Conservation Of Wild Bees In Tree Fruit Orchards, Olivia Mae Kline, Ngoc T. Phan, Mitzy F. Porras, Joshua Chavana, Coleman Z. Little, Lilia Stemet, Roshani S. Acharya, David J. Biddinger, Gadi V.P. Reddy, Edwin G. Rajotte, Neelendra K. Joshi

Entomology and Plant Pathology Faculty Publications and Presentations

Honey bees (Apis mellifera) are the most economically important agricultural pollinator in North America, as well as being the most frequently studied bee species. Many agricultural systems, such as fruit tree orchards, benefit from having a diversity of bee species present. In this article, we present information about the types of bees that can be found in orchards and explore their mating behaviors, life cycles, genetic differences, flower preferences, and foraging activities. Many orchard-pollinating bees, including bumble bees (Bombus spp.), mason bees (Osmia spp.), and mining bees (Andrena spp.), are often less studied than honey …

Revisiting Plant Defense-Fitness Trade-Off Hypotheses Using Solanum As A Model Genus, Sakshi Watts, Satinderpal Kaur, Rupesh Kariyat

Entomology and Plant Pathology Faculty Publications and Presentations

Plants possess physical and chemical defenses which have been found to deter herbivores that feed and oviposit on them. Despite having wide variety of defenses which can be constitutive or induced, plants are attacked and damaged by insects associated with different mouthparts and feeding habits. Since these defenses are costly, trade-offs for growth and defense traits play an important role in warding off the herbivores, with consequences for plant and herbivore growth, development and fitness. Solanum is a diverse and rich genus comprising of over 1,500 species with economic and ecological importance. Although a large number of studies on Solanum …

Metabolic Impact Of Feeding Prior To A 60-Min Bout Of Moderate-Intensity Exercise In Females In A Fasted State, Kayla M. Ratliff, Chad M. Kerksick, Jessica M. Moon, Anthony M. Hagele, Johnathan L. Boring, Kylie Walden, Connor J. Gaige, Richard A. Stecker, Kyle L. Sunderland, Petey W. Mumford

Faculty Scholarship

Background: The metabolic impact of pre-exercise feeding of protein or carbohydrate on fat oxidation and energy expenditure rates, especially, in females, is poorly understood.

Methods: Recreationally active females (n = 15, 32 ± 10 years, 164.8 ± 5.6 cm, 63.5 ± 9.3 kg, 23.4 ± 3.2 kg/m2) completed four testing sessions in a randomized, double-blind, crossover fashion after fasting overnight. Participants ingested isovolumetric and isoenergetic solutions containing either 25 g of whey protein, casein protein, carbohydrate (CHO), or a non-caloric placebo (PLA). Participants then completed 60 min of treadmill exercise at 15% below ventilatory threshold 30 min after ingestion. …

Lipid Binding Properties Of Huntingtin As A Novel Therapeutic Target, Chathuranga Siriwardhana

Graduate Theses, Dissertations, and Problem Reports (ETD)

As protein aggregation is the defining hallmark of all amyloid diseases, a common therapeutic strategy is to develop molecules that inhibit aggregation. However, this approach has yielded limited success. Many amyloid proteins directly interact with lipid membranes. These interactions promote distinct aggregation pathways and often result in membrane damage leading to toxicity. As a result, directly targeting the ability of amyloids to bind lipid membranes represents a novel therapeutic strategy. As a proof of principle, the interaction between lipid membranes and mutant huntingtin protein (htt) aggregates was used to test this strategy. Mutant htt containing an expanded polygulatmine (polyQ) domain …

2023 Weed Science Research Report – Gold Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2023. It includes work on sugarbeet and the management of several weeds in North Dakota.

Investigating The Role Of Nudt7-Mediated Peroxisomal Coa Degradation In The Regulation Of Hepatic Coa Levels And Lipid Metabolism, Schuyler Dan Adams Vickers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Coenzyme A (CoA) is a crucial cofactor required to support many essential metabolic processes, including fatty acid oxidation and synthesis, the TCA cycle, and bile acid synthesis. In order to support the proper functioning of these pathways, CoA levels within the cell must be properly regulated. The regulation of CoA levels is achieved through a balance between CoA synthesis and CoA degradation. While the regulation of CoA synthesis has been extensively characterized, the degradation of CoA has received less attention. As such, the contribution of CoA degradation to the regulation of metabolism is poorly understood. NUDT7 is a CoA-specific Nudix …

Relationships Between Watershed Factors And Community Size Spectra Of The Fish And Macroinvertebrate Communities Of Mid-Appalachian Streams, Jarrett Hunter Landreth

Graduate Theses, Dissertations, and Problem Reports (ETD)

Freshwater lotic communities are complex, dynamic, and quick to respond to changes to their environment. However, some of the specific mechanisms driving those changes are yet to be fully understood. Here, I will use the community size spectrum (CSS) approach to assess how the CSS slope, elevation, and food chain length of the fish and macroinvertebrate assemblages as well as the combined CSS of 15 mid-sized (2nd-4th order) streams in north-central West Virginia respond across gradients of watershed factors (drainage area, mean drainage slope, mean drainage elevation, drainage % forest, and stream specific conductance). Field sampling was carried out in …

Producing Hardwood Cross - Laminated Timber (Hclt) Mats From Low Grade Red Oak Lumber, Jonathan R. Norris

Graduate Theses, Dissertations, and Problem Reports (ETD)

The use of cross-laminated timber (CLT) has significantly grown in North America, but hardwood species have not yet been deemed a viable material to manufacture CLT panels, whereas, softwood species continue to serve as the only approved material for CLT in structural applications according to ANSI/APA PRG-320. In order to introduce hardwoods into the CLT market, we must develop non-structural CLT products that utilize low grade lumber from hardwood species. This allows hardwood manufacturers to enter the CLT market without certification, while also displaying the strength and beauty of the hardwood resources found within this region.

Of the hardwood species …

Spatial Distribution And Insect Associations Of Frangula Alnus In The Allegheny National Forest, Pennsylvania: Implications For Management, Jennifer S. Greenleaf

Graduate Theses, Dissertations, and Problem Reports (ETD)

Glossy buckthorn (Frangula alnus) (Rosales: Rhamnaceae) is an invasive European shrub that threatens to displace native vegetation in roadsides and disturbed forests in North America due to its lack of natural enemies, long leaf-out periods, and its ability to survive in a multitude of soil conditions. While well understood in its native range, information regarding F. alnus in North America is lacking. Currently, the management of this invasive shrub relies on an understanding of where it can be found and thus where treatments should be applied. This research investigated the spatial distribution patterns of F. alnus and profiled …

Rumen And Urine Amine/Phenol-Metabolome Of Beef Steers With Divergent Residual Feed Intake, Taylor S. Sidney

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cattle production is one of the most important agricultural industries in the United States. Despite its overall production efficiency, the industry is facing considerable adversities to its long-term sustainability due to population growth, decreased land availability, and the increase in global warming. Since feed efficiency imposes an immense influence on the cost of production for animals and is a major cost determinant for profitability, the improvement of feed utilization through the understanding of animal-to-animal variation in feed efficiency is pertinent to increase farm productivity and profitability. Residual feed intake (RFI) is a measure of feed efficiency and is defined as …

Elucidating Mechanisms Of Proteasome Modulation By Small Molecules To Treat Neurodegenerative Diseases And Cancer, Janelle Joo Yee Chuah

Graduate Theses, Dissertations, and Problem Reports (ETD)

Maintaining protein homeostasis, or proteostasis, is crucial for cellular function, and protein degradation through the proteasome plays a pivotal role in this process. Dysregulation of the proteasome can lead to proteostasis collapse and has been linked to the development of neurodegenerative diseases and cancers. Neurodegenerative diseases often exhibit proteasome impairment, whereas proteasome upregulation is frequently observed in cancer. Although the use of proteasome inhibitors as a pharmaceutical approach has shown success in certain cancers, it has yet to demonstrate efficacy in neurodegenerative diseases due to the absence of drug-like small molecules capable of effectively reversing proteasome impairment and/or activating the …

The Birds And The Bees And The Willows: Understanding The Reproductive Biology Of Salix Nigra Marsh., Julianne Gmys Grady

Graduate Theses, Dissertations, and Problem Reports (ETD)

Salix nigra is a common tree-form willow found throughout the eastern United States and Canada in riparian and wetland habitats. Like other members of the Salicaceae, it is dioecious with separate male and female individuals. While Salix has been thought to be strictly insect pollinated and Populus wind pollinated, several species of Salix have had wind pollination present in varying amounts by species. S. nigra’s pollination syndrome has not been studied extensively, though it is assumed to be insect pollinated. Unlike other willows with wind pollination, S. nigra is basal on the Salix phylogenetic tree. This study was conducted …

Heat Shock Protein 90 (Hsp90) System In Health And Disease., Daniella Munezero

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cells rely on heat shock proteins (HSP) to facilitate and regulate the folding of the substrate proteins into their native state, and degradation if misfolding cannot be prevented. HSP90, a member of the HSP family, is a potential target for treatment of cancer and neurodegenerative diseases. Unfortunately, several clinical trials for cancer treatment have been discontinued due to cell toxicity accompanying HSP90 inhibition. HSP90 has four distinct but structurally similar paralogs. HSP90 inhibitors target all the paralogs despite increasing proof of functional differences among the paralogs. Understanding the in vivo function of HSP90 and the role played by each paralog …

Application Of Computational Biophysics Techniques To Characterize Cell Membrane-Associated Events, Kyle Billings

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cell membranes are crowded environments which can modulate protein structure-function relationships through interaction with lipids, other proteins, carbohydrate structures and so on. This work focuses the impact of the membrane environment on two varieties of peptides: Microbial rhodopsin proteins, and cyclic peptides.

Life on Earth is dependent on the ability of plants and microbes to harness sunlight for energy production. Their ability to transform light into carbohydrates requires tailor-made machinery, and for a wealth of microorganisms, microbial rhodopsin proteins (MR) are critical for maintaining the concentration gradients used to produce the energy molecule Adenosine triphosphate (ATP). The central retinal molecule …

Evaluating The Effects Of Modifying Mixing And Pelleting Parameters On Feed Quality, Pellet Production Rate, And Broiler Performance, Lucas E. Knarr

Graduate Theses, Dissertations, and Problem Reports (ETD)

In Chapter 2, an experiment was conducted to evaluate the effect of different mixer types (MTY) and mix times (MTI) on mix uniformity (MU). A 2 (mix time) x 3 (mixer type) factorial was utilized in this experiment to create six treatments, each mixed in three randomized complete blocks. Mix times were designated adequate or inadequate, were based on the manufacturers’ recommendation, and varied based on the mixer utilized. The mixer types utilized included a vertical mixer, horizontal mixer, and transfer mixing system. The transfer mixing system can be described as a batch-to-mix system …

Quantity Of Questing Black-Legged Ticks And Associated Micro-Scale Environmental Data Collected From Four Suburban Parks Near New York City, Shipeng Sun, Chong Di, Li Li, Brian Sulkow, Weigang Qiu

Publications and Research

During 2017 and 2018, we collected the quantity of questing black-legged ticks (Ixodes scapularis), also known as deer ticks, in 124 sampling sites of 5m by 5m in four state parks—Caumsett State Historic Park, Connetquot River State Park, Rockefeller State Park, and Fire Island National Seashore—around New York City. The black-legged tick is the primary vector for the spirochete Borrelia burgdorferi, the pathogen of Lyme disease, in Northeastern United States. Using the flagging method, we collected and counted the numbers of adult and nymphal black-legged ticks at each site. Along with these quantities, we also recorded the …

Automated Morphological Phenotyping Using Learned Shape Descriptors And Functional Maps: A Novel Approach To Geometric Morphometrics, Oshane O. Thomas, Hongyu Shen, Ryan L. Raaum, William E. H. Harcourt-Smith, John D. Polk, Mark Hasegawa-Johnson

Publications and Research

The methods of geometric morphometrics are commonly used to quantify morphology in a broad range of biological sciences. The application of these methods to large datasets is constrained by manual landmark placement limiting the number of landmarks and introducing observer bias. To move the field forward, we need to automate morphological phenotyping in ways that capture comprehensive representations of morphological variation with minimal observer bias. Here, we present Morphological Variation Quantifier (morphVQ), a shape analysis pipeline for quantifying, analyzing, and exploring shape variation in the functional domain. morphVQ uses descriptor learning to estimate the functional correspondence between whole triangular meshes …

Evaluating Innovative Methods Of Quantitatively Linking Microbial Community Structure To Ecosystem Function, Jeth Gv Walkup

Graduate Theses, Dissertations, and Problem Reports (ETD)

Microbial functional diversity is the product of community structure and intraspecific trait variation. Due to microbial diversity and limited availability of microbial trait measurements, it has been challenging to quantitatively link community structure to microbial function. Although community-level activity rates vary with community composition, issues in the scale of their measurement inhibit our understanding of their relationship. Quantitative stable isotope probing (qSIP) is a method for quantitatively measuring taxon-specific microbial traits, providing new opportunities to apply trait-based approaches for studying microbial ecology. Quantitative microbial trait data from these experiments enables the functional characterization of microbial communities, potentially enabling the extrapolation …

Krill Protein Fortified Extrudates: A Pilot Study, Avery S. Fleeharty

Graduate Theses, Dissertations, and Problem Reports (ETD)

Antarctic krill (Euphausiacea superba) is an abundant and sustainable but underutilized natural resource with well-studied protein isolation methodology using Isoelectric solubilization-precipitation (ISP). The resulting krill protein isolate (KPI) has high yield and sufficient impurity removal in addition to desirable nutritional and functional properties but has yet to be used in food systems. Extrusion is a versatile and scalable food processing method with many benefits; thus, the aim of this study was to determine the impact of KPI as an additive in extrusion processing with the eventual goal of introducing it to human and animal food systems. KPI was …

Parent And Adolescent Report Of Helicopter Parenting: Examining Reporter Discrepancies And Associations With Adolescent Depression And Anxiety, Avneet Batra

Graduate Theses, Dissertations, and Problem Reports (ETD)

Research is replete with studies on Helicopter Parenting (HP) and its association with depression and anxiety (Cui et al., 2019; Schiffrin et al., 2014; 2019). However, most of the studies have focused on college students’ reports on helicopter parenting and there is relative dearth of studies with adolescents or with parents’ reports of HP (Pistella et al., 2020; Schiffrin et al., 2019). The current study aims to fill the gap in the literature by including both parent and adolescents’ perspectives on helicopter parenting, assess its unique association (parent and teens HP reports) with teen depression and anxiety both concurrently and …

Radiogenomics Of C9orf72 Expansion Carriers Reveals Global Transposable Element Derepression And Enables Prediction Of Thalamic Atrophy And Clinical Impairment, Luke W. Bonham, Ethan G. Geier, Daniel W. Sirkis, Josiah K. Leong, Eliana Marisa Ramos, Qing Wang, Anna Karydas, Suzee E. Lee, Virginia E. Sturm, Russell P. Sawyer, Adit Friedberg, Justin K. Ichida, Aaron D. Gitler, Leo Sugrue, Michael Cordingley, Walter Bee, Eckard Weber, Joel H. Kramer, Katherine P. Rankin, Howard J. Rosen, Adam L. Boxer, William W. Seeley, John Ravits, Bruce L. Miller, Jennifer S. Yokoyama

Psychological Science Faculty Publications and Presentations

Hexanucleotide repeat expansion (HRE) within C9orf72 is the most common genetic cause of frontotemporal dementia (FTD). Thalamic atrophy occurs in both sporadic and familial FTD but is thought to distinctly affect HRE carriers. Separately, emerging evidence suggests widespread derepression of transposable elements (TEs) in the brain in several neurodegenerative diseases, including C9orf72 HRE-mediated FTD (C9-FTD). Whether TE activation can be measured in peripheral blood and how the reduction in peripheral C9orf72 expression observed in HRE carriers relates to atrophy and clinical impairment remain unknown. We used FreeSurfer software to assess the effects of C9orf72 HRE and clinical diagnosis (n …

Opportunities And Challenges In Augmenting Honey Bee Forage Resources With Pasture Legumes In Southern Australia, M. Kratz, M. Brown, M. T. Newell, R. C. Hayes

IGC Proceedings (1977-2023)

More than 80% of Australian honey is produced from native tree and shrub species. However, with increasing demands on public lands along with production risks posed by drought, floods and wildfires, there is a need to identify alternative forage resources to augment Australian honey production. With over 30 pasture legumes now available for agriculture in southern Australia, opportunity exists to increase the utilisation of some species with co-benefits to multiple production industries. However, there is little understanding of the potential value of most pasture legumes for honey production, and side by side comparisons are complicated by factors such as differences …

Data Curation For Modeling Tall Fescue Biomass Dynamics With Dssat-Csm, M. B. Hanson, P. D. Alderman, T. J. Butler, A. Caldeira Rocateli

IGC Proceedings (1977-2023)

While models for predicting forage production are available to aid management decisions for some forage crops, there is limited research for a yield model designed specifically for tall fescue (Schedonorus arundinaceus). Therefore, our objective was to adapt an existing perennial forage model, the Decision Support System for Agrotechnology Transfer Cropping Systems Model (DSSAT-CSM) for predicting forage biomass of tall fescue in the southern Great Plains. To evaluate model performance, there must first be a high level of data manipulation and cleaning. In this project, a cohesive dataset combining biomass, weather, soil, and management data were structured into DSSAT …

Can Changing Breeding Structure Serve As A Climate Adaptation Strategy? Evidence From A Comparative Study Of China And Mongolia, Y. Zhang, Z. Huang, J. Wang, Y. Huang, T. Hao, G. Xu

IGC Proceedings (1977-2023)

Under the influence of climate change, the vulnerability of grassland ecosystems in the Mongolian Plateau has increased. Correspondingly, herdsmen need to adopt more livelihood adaptation strategies to cope with changes in climate and grassland environment. The purpose of this study is to analyze the impact of breeding structure on grassland quality in Mongolian plateau. We select 20 border provinces/banners of Mongolia and Inner Mongolia Autonomous Region for research, and combined statistical data with remote sensing data of 2007-2016. The Normalized Difference Vegetation Index (NDVI) is used to quantify the grassland vegetation quality. Results show that there are differences in livestock …

Land Systems, Soils And Vegetation Of The Southern Goldfields And Great Western Woodlands Of Western Australia - Volume 2, Peter-Jon A. Waddell, Paul Galloway

Technical Bulletins

This technical bulletin defines and maps the land resources of the southern Goldfields region of Western Australia (WA). The southern Goldfields region, as featured in this bulletin, covers 151,753 km². The western and eastern borders are variable because they align with various cadastral boundaries. The western survey border, which in part follows the WA State Barrier Fence, demarcates cleared agricultural land from the intact, western extent of the Great Western Woodlands. The eastern border abuts the westernmost Nullarbor pastoral lease boundaries, except for the north-easternmost and south-easternmost edges which continue into Crown land. Major towns are Coolgardie, Kalgoorlie …

Placing The Evolutionary History Of Desmognathus Salamanders In Context: A Phylogeographic Approach, Kara Jones

Theses and Dissertations--Biology

Patterns of genetic variation do not arise in a vacuum but are instead shaped by the interplay between evolutionary forces and ecological constraints. Here, I use a phylogeographic approach to examine the role that ecology played in lineage divergence in the Desmognathus quadramaculatus species complex (Family: Plethodontidae), which consists of three nominal species: D. quadramaculatus, D. marmoratus, and D. folkertsi. Previous phylogenetic studies have shown that individuals from these species do not form clades based on phenotype. My approach to reconciling phylogenetic discordance was two-fold, using (1) genome-wide markers to provide insight into the …

Examining The Effects Of Hyperglycemia On Retinal Regeneration, Emmanuella Ec Kyllians

Theses and Dissertations--Biology

Diabetes is a disease that affects how the body processes glucose, leading to a cascade of other complications, one of which is diabetic retinopathy (DR). DR is a neurovascular disease that affects the retina and research has revealed the role of retinal neurodegeneration in its development. Animal models like zebrafish have emerged as important tools for studying the molecular and cellular mechanisms underlying DR. On the other hand, retinitis pigmentosa (RP) is a degenerative disease that involves the progressive degeneration of rods and cones, and hyperglycemia has been found to impair regeneration. Therefore, this study aims to examine the effects …

Competition And Herbivory Influence The Survival, Growth, And Physiology Of Native Tree Seedlings In The Kentucky Inner Bluegrass Savanna-Woodland, James D. Shaffer

Theses and Dissertations--Biology

Terrestrial plant communities are shaped by competition for resources, herbivory, and abiotic processes. Savanna systems represent a dynamic coexistence of contrasting life forms (grasses and trees) shaped by competition and disturbance. The Kentucky Inner Bluegrass Savanna-Woodland (KIBSW) is described as an open woodland of shade intolerant species; however, climatic, and edaphic conditions can support closed-canopy forest. After European pioneer settlement (c1750-1800), over 99% of “savanna-woodlands” have been lost. KIBSW remnants are experiencing a recruitment failure, leading to a dominance shift in tree communities. I researched how tree-grass competition and mammalian herbivory influence KIBSW regeneration and maintenance. The KIBSW does not …