Life Sciences Commons^™

Ovarian Inflammation Mediated By Toll-Like Receptor 4 Increased Transcripts Of Maternal Effect Genes And Decreased Embryo Development, Alison F. Ermisch, Katie L. Bidne, Scott G. Kurz, Kerri A. Bochantin, Jennifer R. Wood

Department of Animal Science: Faculty Publications

Obese women are subfertile and have reduced assisted reproduction success, which may be due to reduced oocyte competence. We hypothesize that consumption of a high-fat/high-sugar diet induces ovarian inflammation, which is a primary contributor to decreased oocyte quality and pre-implantation embryo development. To test this hypothesis, C57BL/6 (B6) mice with a normal inflammatory response and C3H/HeJ (C3H) mice with a dampened inflammatory response due to dysfunctional Toll-like receptor 4 were fed either normal chow or high-fat/high-sugar diet. In both B6 and C3H females, high-fat/high-sugar diet induced excessive adiposity and hyperglycemia compared to normal chow-fed counterparts. Conversely, ovarian CD68 levels and …

Evaluating Draft Environmental Protection Agency Emissions Models For Broiler Operations, G. Li, R. S. Gates, B. C. Ramirez, R. T. Burns

Department of Animal Science: Faculty Publications

In August 2021, the U.S. Environmental Protection Agency( EPA) released draft models to estimate daily NH3, H2S, PM2.5, PM10, and total suspended particulate semissions from U.S. broiler operations using inputs of daily mean ambient temperature, relative humidity, and live animal weight. The EPA developed these models using data sets collected between 2005 and 2007. It is important to note that over 15 yr have passed since the original data were collected, and broiler genetics, nutrition, and management have improved consideably. These models, if adopted, could have substantial impact on the U.S. broiler industry regarding air emission management and regulation. The …

Benefits Of A Family-Based Judo Program For Parents Of Children With Autism Spectrum Disorder, Jeslin George

Honors Undergraduate Theses

A diagnosis of Autism Spectrum Disorder (ASD) can impact the entire family. Parents of children with ASD reportedly have greater stress levels, family conflict, financial concerns, and poor health habits than parents of neurotypical (NT) children. While many parent-focused interventions have been developed, these interventions focus on parent training and child behavior outcomes rather than the health and well-being of the parents. Therefore, this study aimed to examine the effects of a 15-week family judo program on physical and psychosocial health in parents of children with ASD. A total of 18 parents of children with ASD participated in a weekly …

The Effect Of E-Cigarette Vape On Oral Cell Proliferation Using 3d Spheroids As A Preclinical Model, Vikram Chinnaiyan

Honors Undergraduate Theses

E-cigarettes have recently become increasingly popular, especially amongst middle and high school students. Although they are marketed as safer alternatives to tobacco cigarettes, they produce toxic metals and carcinogenic nitrosamines. This thesis studies the effects of e-cigarette aerosol on the growth and proliferation of oral epithelial cells because the consequences of vaping, including a potential risk for aberrant growth leading to cancer, are not well understood. Cells were grown in matrigel, causing the formation of three-dimensional spheroids modeling the physiological architecture of the oral epithelium. Those spheroids were chronically exposed to vape with different treatment conditions to study the functional …

Ser14-Rpn6 Phosphorylation Mediates The Activation Of 26s Proteasomes By Cyclic Amp And Protects Against Cardiac Proteotoxic Stress In Mice, Liuqing Yang

Dissertations and Theses

A better understanding of how proteasome activity is regulated can facilitate the search for proteasome enhancement strategies for disease treatment. A cell culture study shows cAMP-dependent protein kinase (PKA) activates 26S proteasomes by phosphorylating Ser14 of RPN6 (pS14-RPN6), but this discovery and its physiological significance remain to be established in vivo. To test the hypothesis that pS14-RPN6 mediates the activation of proteasomes by PKA and reduces proteotoxicity in animals, two knock-in mouse models with Ser14 of endogenous Rpn6 mutated to either Ala (S14A) or Asp (S14D) to respectively block or mimic pS14-Rpn6 were created. In a PKA-dependent manner, cAMP augmentation …

Characterisation Of Selected Microbes In Terrestrial And Simulated Extraterrestrial Habitats: Human Health And Environmental Applications, Daniel Joseph Keaney

Theses

The rapid development of the Space industry and associated technologies over the past decade is leading to the inevitable consideration of establishing human-based colonies on extra-terrestrial bodies such as the moon and Mars. With Mars being the focus of not only this body of work, but current New Space Age travel (NASA and SpaceX), a significant issue posed by Martian colonists includes establishing a safe and sustainable food source. Owing to its constantly evolving prehistoric landscape, the soils of Mars are contaminated with sodium perchlorate, a water-soluble salt that is toxic to humans, leftover from once inferred brine seeps that …

Upper Crustal Structure Of Superfast-Spread Oceanic Crust Exposed At The Pito Deep Rift: Implications For Seafloor Spreading, J. A. Karson, L. A. Chutas, N. W. Hayman, R. N. Hey, A. J. Horst, S. D. Hurst, E. M. Klein, D. F. Naar, R. J. Varga

Marine Science Faculty Publications

A tectonic window into the upper 2,000 m of oceanic crust generated at the superfast spreading (∼142 mm/yr) southern East Pacific Rise exposes a continuous layered structure of basaltic lavas and sheeted dikes over gabbroic rocks. This relatively simple structure is in accord with expectations for crustal accretion at a very fast spreading rate and high magma budget where magmatic construction keeps pace with plate separation. Detailed observations show that basaltic lava flows dip progressively more steeply inward (toward the spreading axis where they were erupted). Underlying sheeted dikes are faulted and tectonically rotated to dip steeply outward. These structures …

Microalgal Cultivation Characteristics Using Commercially Available Air-Cushion Packaging Material As A Photobioreactor, Clifford R. Merz, Neha Arora, Michael Welch, Enlin Lo, George P. Philippidis

Marine Science Faculty Publications

Air-cushion (AC) packaging has become widely used worldwide. ACs are air-filled, dual plastic packaging solutions commonly found surrounding and protecting items of value within shipping enclosures during transit. Herein, we report on a laboratory assessment employing ACs as a microalgal photobioreactor (PBR). Such a PBR inherently addresses many of the operational issues typically encountered with open raceway ponds and closed photobioreactors, such as evaporative water loss, external contamination, and predation. Using half-filled ACs, the performance of microalgal species Chlorella vulgaris, Nannochloropsis oculata, and Cyclotella cryptica (diatom) was examined and the ash-free dry cell weight and overall biomass productivity determined …

Comprehensive Datasets Of The Cariaco Ocean Time-Series Program (1995-2017) In The Southeastern Caribbean Sea, Frank Muller-Karger, Yrene Astor, Ramon Varela, Robert Thunell, Mary Scranton, Gordon Taylor, Kristen Buck, Kent Fanning, Claudia Benitez-Nelson, Laura Lorenzoni, Enrique Montes, Eric Tappa, Luis Troccoli, Rafael Diaz-Ramos, Sonia Subero-Pino, Jaimie Rojas, Luis Gonzalez, Javier Gutierrez, Jesus Narvaez, Alberto Rosales, Laurencia Guzman, Juan Capelo, Digna Rueda-Roa

Marine Science Faculty Publications

The Cariaco Basin, located off eastern Venezuela in the southeastern Caribbean Sea, is the world's largest marine anoxic basin and the site of one of the longest-running oceanographic studies on record. The CARIACO Ocean Time-Series Program collected various oceanographic parameters from 1995 until 2017 at the Cariaco station (10.50° N, 64.67° W). The Cariaco Basin exhibits pronounced seasonal and interannual variations in hydrographic properties and primary production due to seasonal coastal upwelling. Additionally, due to its restricted circulation and high primary production, the basin is anoxic below approximately 250 meters.

Monthly oceanographic cruises were conducted aboard the R/V Hermano Ginés …

Meta-Analysis Of The Effects Of Insect Pathogens: Implications For Plant Reproduction, Wilnelia Recart Gonzalez, Rover Bernhard, Isabella Ng, Katherine Garcia, Arietta E. Fleming-Davies

Biology: Faculty Scholarship

Despite extensive work on both insect disease and plant reproduction, there is little research on the intersection of the two. Insect-infecting pathogens could disrupt the pollination process by affecting pollinator population density or traits. Pathogens may also infect insect herbivores and change herbivory, potentially altering resource allocation to plant reproduction. We conducted a meta-analysis to (1) summarize the literature on the effects of pathogens on insect pollinators and herbivores and (2) quantify the extent to which pathogens affect insect traits, with potential repercussions for plant reproduction. We found 39 articles that fit our criteria for inclusion, extracting 218 measures of …

Forensic Microbiome Evidence: Fourth Amendment Applications And Court Acceptance, Trason Lasley

Catholic University Journal of Law and Technology

No abstract provided.

Combined 3d Bioprinting And Tissue-Specific Ecm System Reveals The Influence Of Brain Matrix On Stem Cell Differentiation, Martina Zamponi, Peter A. Mollica, Yara Khodour, Julie S. Bjerring, Robert D. Bruno, Patrick C. Sachs

School of Medical Diagnostics & Translational Sciences Publications

We have previously shown that human and murine breast extracellular matrix (ECM) can significantly impact cellular behavior, including stem cell fate determination. It has been established that tissue-specific extracellular matrix from the central nervous system has the capacity to support neuronal survival. However, the characterization of its influence on stem cell differentiation and its adaptation to robust 3D culture models is underdeveloped. To address these issues, we combined our 3D bioprinter with hydrogels containing porcine brain extracellular matrix (BMX) to test the influence of the extracellular matrix on stem cell differentiation. Our 3D bioprinting system generated reproducible 3D neural structures …

Cleome Serrulata, Rocky Mountain Beeweed, Uses By Native Americans, Marielle Gomez, John Ubelaker

Research

This paper examines the use of beeweed (Cleome serrulata), a native annual commonly called Rocky Mountain beeplant, as a traditional dye and paint source for pottery in Northern New Mexico.

Behavioral, Morphological And Genetic Differentiation Among Populations Of Enyaliopsis, Laura Macamo

Electronic Theses and Dissertations, 2020-2023

The study of biodiversity is a fundamental part of the study of the ecosystem's function and stability and how changes in the habitat shape the interaction between species. In recent years, the classification of species benefited from the analysis of several types of data to aid the distinction of morphologically similar species. Enyaliopsis iaculator (Naskrecki and Guta, 2019) is a species endemic to central Mozambique. Different populations of E. iaculator were reported to exhibit differences in the time of the day the mating call is displayed, but no morphological differences were reported. Since call behavior tends to be species specific …

Copy Number Alterations Of Aggressive Pituitary Neuroendocrine Tumors, Grace Zhang

Scripps Senior Theses

Pituitary neuroendocrine tumors (PitNETs) are tumors of the pituitary gland. Although most are benign, they can cause severe morbidity if compression of surrounding tissue and/or endocrinopathies occur.

Aggressive PitNETs are notably detrimental and difficult to predict, and their effects are further exacerbated by challenges in treatment. Although histological studies can detect certain markers of tumor aggressiveness, they are insufficient at wholly predicting PitNET aggressiveness, making the clinical behavior of PitNETs challenging to determine. Since treatment of aggressive tumors also remains suboptimal, this further results in negative impacts on health and quality of life.

Genetic markers, such as copy number variations …

Gene Expression Under Combined Hypoxia And Acidosis In Chondrosarcoma, Michael Stacey, Kostika Vangjeli, Christopher Osgood

Bioelectrics Publications

Chondrosarcomas are the second most common cause of bone cancer and are removed surgically with wide margins. On recurrence, they are resistant to chemo and radiation therapy and new treatment options are critically required. This tumor type produces hyaline cartilage, a cartilage normally formed under hypoxic and acidic environment due to lack of vasculature in cartilage. Paradoxically, chondrosarcomas arise in the well vascularized, oxygen rich environment of the bone. Hypoxia and acidosis are two stressors where the cellular effects are typically reported separately even though cells experience combined effects of hypoxia and acidosis. Given the mechanistic links between hypoxia and …

The Role Of Myocardin In The Progression Of Non-Small Cell Lung Cancer, Soromidayo Akinsiku

Biotechnology Theses

Lung cancer is the leading cause of cancer-related mortality in the world and NSCLC accounts for 85% of all lung cancer cases. The mainstay of treatment for patients with stage I, II and IIIA NSCLC is surgery, followed by post-operative cisplatin-based chemotherapy. Additional adjuvant therapy involving targeted tyrosine kinase inhibitors has been in use, however even for the targeted therapy, resistance eventually develops. Therefore, there is a need for identifying novel targets for this life-threatening disease. Given that preliminary studies in Ikebe lab revealed that myocardin knockdown significantly promoted caspase-3 degradation, in this study, using myocardin siRNA, we investigated the …

1-Back Matching And Mismatching By Pigeons, Thomas Zentall

Psychology Research Data

No abstract provided.

International Society Of Sports Nutrition Position Stand: Nutritional Considerations For Single-Stage Ultra-Marathon Training And Racing, Stacy T. Sims, Chad M. Kerksick, Abbie E. Smith-Ryan, Xanne A.K. Janse De Jonge, Katie R. Hirsch, Shawn M. Arent Ph.D., Cscs*D, Fissn, Facsm, Fnak, Susan Joyce Hewlings, Susan M. Kleiner, Erik Bustillo, Jaime L. Tartar, Valerie G. Starratt, Richard B. Kreider, Casey Greenwalt, Liliana I. Rentería, Michael J. Ormsbee, Trisha A. Vandusseldorp, Bill I. Campbell, Douglas S. Kalman, Jose Antonio

Faculty Publications

Based on a comprehensive review and critical analysis of the literature regarding the nutritional concerns of female athletes, conducted by experts in the field and selected members of the International Society of Sports Nutrition (ISSN), the following conclusions represent the official Position of the Society: 1. Female athletes have unique and unpredictable hormone profiles, which influence their physiology and nutritional needs across their lifespan. To understand how perturbations in these hormones affect the individual, we recommend that female athletes of reproductive age should track their hormonal status (natural, hormone driven) against training and recovery to determine their individual patterns and …

Building A High-Throughput Yeast Two-Hybrid Assay To Screen For Novel Chronic Hepatitis B Drugs, Benjamin Glazer

Lawrence University Honors Projects

Hepatitis B is a vaccine preventable liver disease caused by hepatitis B virus (HBV). Chronic hepatitis B currently affects about 296 million people around the world, killing 820,000 annually. There is currently no cure for chronic hepatitis B, but research over the last decade has identified hepatitis B X protein (HBx) as a promising therapeutic target. Specifically, inhibiting the interaction between HBx and human protein DDB1 could disrupt HBx function. An essential step in the drug development process is to create an assay that is capable of high-throughput screening millions of compounds for activity against HBx-DDB1. This research aims to …

Investigating Neuroanatomical Evolution Through Proliferative Cells In The Astyanax Mexicanus, Sophie Staeger

Pitzer Senior Theses

The Astyanax mexicanus is a single species of fish that has two very distinct morphs, the cave fish and the surface fish. 200,000 years ago, cave fish ancestors entered the dark and challenging cave environment of Mexico and were forced to adapt in order to survive. Such adaptions include larger fat reserves, more sensitive olfaction, increased neuromasts, and a unique nervous system structure. Yet, other functions regressed, as they lost their eyes and pigmentation. The Duboué lab has established a clear reciprocal relationship between the volume of structures involved in the neuronal adaptations, but the biological mechanisms underlying the development …

Restoring An Iconic Joshua Tree Forest: Natural Recovery, Restoration Success, And Soil Microhabitats On A Desert Burn Area, Riley Scaff

Pitzer Senior Theses

Wildfires are a novel disturbance type in the Mojave Desert, but have been increasing in size and frequency since the 1970s due to the proliferation of invasive grasses. Burned areas are more susceptible to invasion, which can lead to the establishment of a grass-fire cycle. For this reason, restoration is often necessary to facilitate natural plant community recovery, but the extreme desert climate makes returning native vegetation to burn areas challenging. Planting in nutrient-rich soil microhabitats could be a helpful tool to improve revegetation success, but has yet to be tested. This study surveyed natural postfire recruitment across two seasons …

Cultivating Carrots, Community, And Health-Conscious Children: Investigating The Effects Of Community Garden Implementation On Nutrition Attitudes Of Elementary Students In The Los Angeles Unified School District, Hannah Michele Tiedemann

Scripps Senior Theses

Growing up in an urban food desert can significantly affect children's development, health, and well-being (Jencks et al., 1990; Leventhal et al., 2000). Compared to their more affluent peers, youth living in low-socioeconomic urban neighborhoods are at greater risk of experiencing poor-quality diets, food insecurity, unhealthy body weights, and mental health problems (Duncan et al., 1997; Sampson et al., 1997). Moreover, children living in food-insecure households are more likely to consume calorically dense diets high in trans fat and added sugar, putting them at risk for poor health, childhood obesity, and chronic, diet-related diseases as adults (Nielsen et al., 2002). …

The Effects Of Rootstocks On Growth And Development Of Sour Cherry (Prunus Cerasus L. Cv. "Kütahya") In The Growing Conditions Of Bursa, Mevlüt Batuhan Koşar, Di̇lan Ahi Koşar, Ümran Ertürk

Turkish Journal of Agriculture and Forestry

Turkey is one of the most important sour cherry (Prunus cerasus L.) producers in the world, mostly producing the cultivar "Kütahya." To date, no previous study published on the effects of clonal roodstocks on the growth and development, yield, and fruit quality of ?Kütahya? sour cherry. Therefore, this study was carried out from 2015 to 2019 to determine the influences of SL-64, Piku 3, Maxma 14, CAB 6P and PHL-C rootstocks on vigor, yield, and fruit quality of "Kütahya" sour cherry cultivar in Bursa, Marmara Region, Turkey. The effects of rootstocks on blooming period, ripening time, trunk diameter, trunk …

Chefs’ Perspectives Of Failures In Foodservice Kitchens, Part 2: A Phenomenological Exploration Of The Consequences And Handling Of Food Production Failure, Mohamed Fawzi Afifi, J.J. Healy, Mairtin Mac Con Iomaire

Articles

This paper explores the consequences of food production failure (FPF) and its handling in foodservice operations from the perspective of chefs. A phenomenological epistemology and qualitative methodology were followed. Fifteen semi-structured interviews with chefs working in independent restaurants and hotels were carried out using purposive sampling, and employing an emic posture. Interviews were transcribed verbatim, read repetitively, and coded. Thematic analysis yielded themes on the consequences of FPF, on operation and staff, handling failures with kitchen staff, front of the house (FOH), and management. The findings revealed that the major ramification of FPF is financial through food loss. Representing both …

Efficient Text Bounding Box Identification Using Mask R-Cnn: Case Of Thai Documents, Phanthakan Kiatphaisansophon

Chulalongkorn University Theses and Dissertations (Chula ETD)

Text detection is a fundamental task in computer vision, particularly for Optical Character Recognition (OCR) application. This research focuses on text detection part of the OCR. Our previous text detection model, CRAFT (Character-Region Awareness For Text detection) has shown promising outcomes in bounding box identification. Nevertheless, it encounters issues associated with post-processing and multiline text. The post-processing related to the necessity to reconfigure the model when new documents are introduced, resulting in inefficiencies and complexities. Also the CRAFT model tends to blend bounding boxes from consecutive lines, introducing errors and impacting the accuracy in the text recognition part of the …

การเกิดภาวะพหุสัณฐานทางพันธุกรรมของยีนในวิถีวิตามินดีและความเสี่ยงต่อภาวะออทิซึมสเปกตรัมในประชากรไทย, ชาญณรงค์ แซ่ฉั่ว

Chulalongkorn University Theses and Dissertations (Chula ETD)

กลุ่มอาการออทิซึมสเปกตรัม (Autism spectrum disorder; ASD) เป็นปัญหาสุขภาพระดับโลกที่สำคัญ โดยพบว่ามีความชุกของโรคที่เพิ่มสูงขึ้น อย่างไรก็ตามสาเหตุและกลไกในการเกิดภาวะออทิซึมสเปกตรัมยังไม่ทราบแน่ชัดอาจเกี่ยวข้องกับปัจจัยด้านพันธุกรรมและสิ่งแวดล้อม โดยปัจจุบันมีรายงานถึงบทบาทของวิตามินกับภาวะออทิซึมสเปกตรัม วัตถุประสงค์ของการศึกษานี้คือศึกษาการเกิดภาวะพหุสัณฐานทางพันธุกรรมของยีนในวิถีเมทาบอลิกของวิตามินดีและความเสี่ยงต่อการเกิดภาวะออทิซึมสเปกตรัมและระดับความรุนแรงของโรคในเด็กไทย การศึกษานี้มีจำนวนอาสาสมัคร 276 ราย โดยเป็นกลุ่มเด็กที่มีภาวะออทิซึมสเปกตรัม 169 ราย และกลุ่มควบคุมเป็นเด็กที่ไม่มีโรคทางระบบประสาท 107 ราย ซึ่งได้ทำการตรวจวิเคราะห์ภาวะพหุสัณฐานทางพันธุกรรมของยีน CYP2R1, CYP27B1, GC และ VDR โดยเทคนิค TaqMan-based real-time Polymerase Chain Reaction (PCR) และตรวจวิเคราะห์ระดับวิตามินดีในกระแสเลือดโดยใช้เทคนิค Chemiluminescence Immunoassay (CLIA) โดยเด็กที่พบภาวะออทิซึมสเปกตรัมจะได้รับการตรวจระดับความรุนแรงของภาวะออทิซึมสเปกตรัมด้วย Childhood Autism Rating Scale, 2nd Edition (CARS2) โดยผลที่ได้จากการศึกษาพบว่า การเกิดภาวะพหุสัณฐานทางพันธุกรรมของยีน CYP2R1, CYP27B1, VDR และ GC (rs4588) ไม่มีความสัมพันธ์กับการเกิดภาวะออทิซึมสเปกตรัมและความรุนแรงของอาการออทิซึมสเปกตรัม อย่างไรก็ตามการศึกษาชี้ให้เห็นว่า การเกิดภาวะพหุสัณฐานทางพันธุกรรมของยีน GC (rs7041 (A>C)) มีความสัมพันธ์อย่างมีนัยสำคัญกับความเสี่ยงที่เพิ่มขึ้นต่อการเกิดภาวะออทิซึมสเปกตรัมและความรุนแรงของอาการออทิซึมสเปกตรัมด้วย โดยเฉพาะอย่างยิ่งบุคคลที่มีการขาดระดับวิตามินดีร่วมด้วย นอกจากนี้ผลศึกษาพบว่าไอโซฟอร์มของ GC1s และ GC1s-GC1s ฟีโนไทป์มีความสัมพันธ์อย่างมีนัยสำคัญกับความเสี่ยงต่อการเกิดภาวะออทิซึมสเปกตรัมตลอดจนความรุนแรงของอาการออทิซึมสเปกตรัม โดยสรุปการศึกษาวิจัยในครั้งนี้ชี้ให้เห็นว่า การเกิดภาวะพหุสัณฐานทางพันธุกรรมของยีน GC (rs7041 (A>C)) และไอโซฟอร์ม GC1s อาจเป็นตัวบ่งชี้ทางพันธุกรรมที่สามารถช่วยในการประเมินความเสี่ยงต่อการเกิดภาวะออทิซึมสเปกตรัมและความรุนแรงของอาการออทิซึมสเปกตรัมในเด็กไทยได้ในอนาคต อย่างไรก็ตามการศึกษาในอนาคตควรศึกษาในกลุ่มประชากรที่ใหญ่ขึ้นเพื่อยืนยันผลการศึกษาวิจัยครั้งนี้

Rectal Temperature And Respiration Rate As Indicators Of Heat Stress In Broiler Chickens Subjected To Early-Age Thermal Conditioning And Vitamin C Supplementation, Zoran Ruzic, Zdenko Kanacki, Slobodan Stojanovic, Zorana Kovacevic, Slobodan Knezevic, Srdan Todorovic, Smiljana Paras

Turkish Journal of Veterinary & Animal Sciences

One of the major concerns in poultry production presents heat stress (HS) and it is followed by significant economic loss manifested by reduced growth, decreased immune response, increased mortality, etc. The objective of this study was to investigate whether the early-age thermal conditioning (ETC) and vitamin C (Vit.C) treatments, individually and in combination, produce their beneficial effects through increased heat release manifested as a change in respiration rate consequentially leading to a decrease in the chicken's body temperature and thus reduce the harmful effects of HS. A 400 broiler chicks mixed sex male and female were split into four groups. …

Développement De Stratégie Visant À Contrôler Le Niveau De Contamination Des Piscines Par Les Sous-Produits De Désinfection, Maximilien Debia, Elham Ahmadpour, Isabelle Valois, Sami Haddad, Robert Tardif, Stéphane Hallé, Hélène Proulx, Manuel Rodriguez, François Proulx, Ianis Delpla, Jean Sérodes, Sabrina Simard

Rapports de recherche scientifique

La présence de sous-produits de désinfection dans l’eau et l’air des piscines intérieures est une problématique de santé publique et de santé au travail. L’exposition aux sous-produits de désinfection et notamment à la trichloramine est associée à différents effets délétères comme l’irritation des voies respiratoires, l’irritation oculaire et l’hyperréactivité bronchique. La présence des sous-produits de désinfection résulte des réactions chimiques qui surviennent entre les agents désinfectants ajoutés et les matières organiques ou azotées présentes naturellement dans l’eau ou qui y sont amenées par les baigneurs.

L’objectif général de recherche visait à améliorer les connaissances sur les pratiques de gestion et …

Extracting High-Molecular Weight Dna From Cyanobacteria Using Promega's Wizard® Hmw Dna Extraction Kit With A Modified Protocol, Metis, Megan A. Hept, Lesley H. Greene

Chemistry & Biochemistry Faculty Publications

Extraction of high molecular weight (HMW) DNA for long read sequencing with little to no fragmentation and high purity is difficult to acquire from cyanobacterial species. Here we describe a modified method of extraction using Promega's Wizard® HMW DNA Extraction Kit to acquire high molecular weight DNA from cyanobacterial species. The protocol used in the kit is the “3.D. Isolating HMW DNA from Gram-Positive and Gram-Negative Bacteria” protocol. During a key step in the protocol, the lingering remnants of the mucilage layer of the cyanobacterial species is removed, preventing it from sticking to the DNA pellet produced. This customized modification …