Life Sciences Commons^™

Kom-Butcher Of Pathogens, Mel Peña, Yazmin Chamu, Laura Lea Davis

Humboldt Journal of Microbiology

The antimicrobial properties of kombucha are not well understood, but it is believed that they can be used against foodborne pathogens. In this study, its effectiveness was tested against two common food-borne and one yeast pathogens (E. coli, S. aureus, and C. auris). Three popular store-bought kombuchas (GT Synergy, Moss Beach, and Health-Aide Raw) and one home-brewed kombucha were used to study the antimicrobial properties of kombucha. Several nutrient broth tubes were individually inoculated with the microbes in question and were treated with kombucha (Figures 1-3). There were indications of bacteria settling at the bottom of all tubes except the …

Effects Of Fasting On The Oral Microbiome, Youyuan Wang, Leonard Moreno, Fernando Reyes

Humboldt Journal of Microbiology

Humans are mostly made up of microbes. Multiple studies have estimated that bacterial cells outnumber human cells in the body, with our digestive systems harboring the highest concentrations of bacterial life. The oral microbiome is densely populated and of significant physiological importance. These microbial communities maintain tooth and gum health, aids in pre-digestion of foods, and even plays a defensive role — guarding their host from external threats that may lead to oral and systemic pathologies. Studies have shown that fasting can affect the gut microbiome, but there is currently insufficient research on how fasting intervals can affect the oral …

Diatom Abundance And Diversity Across The Arcata Wastewater Treatment Plant And Wildlife Sanctuary, Rhiannon Red Bird, Anna K. Curry, Brandy R. Ayon, Kellie N. Pierson

Humboldt Journal of Microbiology

Wastewater management is an essential component of modern society that is necessary for reducing human environmental impact, particularly in aquatic ecosystems. In Humboldt County, California, the Arcata Wastewater Treatment Plant and Wildlife Sanctuary (Arcata Marsh) is a model for other wastewater treatment plants worldwide to treat waste naturally, reducing the use of chemicals. This wastewater is then discharged into the Humboldt Bay. It is well known how treatment helps remove harmful waste materials, but it has not been investigated how phytoplankton diversity is affected before, during, and after treatment. Phytoplankton are primary producers that are responsible for carbon sequestration and …

Full Issue

Humboldt Journal of Microbiology

No abstract provided.

Kirby-Bauer Disc Diffusion Method Indicates Absence Of Antimicrobial Properties In Ariolimax Columbianus Mucus, Elsa Balfe, Elizabeth Kowalski, Stephanie A. Leavitt

Humboldt Journal of Microbiology

Antibiotic resistance is a rapidly accelerating epidemic demanding novel approaches. Gastropod mucus has been shown to possess antimicrobial properties and could potentially be used as an ingredient in antibiotic development. However, whether the mucus of Ariolimax columbianus, the banana slug, also displays antimicrobial properties is unknown. In this study, we investigated whether the mucus of A. columbianus is resistant to Escherichia coli (E.coli), Streptococcus aureus (S.aureus), and Klebsiella pneumoniae (K.pneumoniae), three medically relevant strains of bacteria. Specimens were collected from a coniferous forest and isolated for downstream mucus extraction. We spread uniform concentrations of our bacteria on Mueller-Hinton agar plates …

Fostering Socio-Ecological Resilience To Wildfire By Interconnecting Knowledge Systems At Cal Poly Humboldt, Jeffrey M. Kane, Erin Kelly, Benjamin Graham, David Greene

Humboldt Journal of Social Relations

The wildfire-related challenges of Northern California and many other regions in the western United States are daunting in scope and magnitude. Ecologically and culturally salient solutions that limit the negative impacts of wildfire and promote resilience of human and ecological systems will require newer approaches. Through Cal Poly Humboldt and the Fire Resilience Institute, there is greater emphasis on the interconnection of knowledge systems across education, training, research, and management. Here we highlight several on-going efforts that seek to enhance the fire resilience workforce, promote socio-ecological resilience through interdisciplinary projects, and inform management through monitoring and research projects that intentionally …

S-Acylation Is A Key Regulator Of Orai1/Stim1-Mediated Store-Operated Calcium Entry In T Cells, Savannah J. West Diaz

Dissertations and Theses (Open Access)

Orai1 and STIM1 proteins are the essential components of the Ca²⁺ release activated Ca²⁺ (CRAC) channel which is required for store-operated Ca²⁺ entry (SOCE) in T cells and subsequent signaling events leading to T cell activation, proliferation, and differentiation. Plasma membrane (PM)-localized Orai1 is the pore-forming subunit of the CRAC channel, and STIM1 is the Ca²⁺ sensor localized to the endoplasmic reticulum (ER) membrane in quiescent T cells. T cell receptor (TCR) stimulation leads to depletion of ER Ca²⁺ stores resulting in Ca²⁺ no longer being bound to STIM1. This activates STIM1 by triggering …

Identifying Stable Isotope Patterns Among Taxa, Sites, And Environmental Variables In The Eastern Mississippi Sound, Evan C. Marth, Carl S. Cloyed, Ruth H. Carmichael

Gulf and Caribbean Research

No abstract provided.

Investigating Smooth Muscle Myosin Dynamics And Assembly In Physiology And Pathology, Maggie Bennett

Dissertations

Vascular smooth muscle cells (SMCs) surround many blood vessels throughout the body,where they dynamically alter vessel diameter to regulate blood pressure, provide structural integrity, and absorb shock on a beat-to-beat timescale. As smooth muscle function fails, profound vascular disease ensues, often with tragic results- even death. Smooth muscle myosin 2 (SM2) is the dominant motor protein that actuates contractility and allows SMCs to perform these vital functions. To function, SM2 monomers dynamically assemble into filaments, which upon SMC activation, associate with filamentous actin to drive contractility. Despite the critical contribution of SM2 to SMC function, foundational aspects of SM2 assembly …

An Analysis Of Department Of The Air Force Bird Strikes And Precipitation, Jason Powell Mas, Cfmei, Atp

International Journal of Aviation, Aeronautics, and Aerospace

This study explored the relationship between precipitation, the amount of time between precipitation events, bird strike risk, and the agricultural zone related to Department of the Air Force (DAF) aircraft operations. Certain bird species demonstrated a relationship between agricultural zones, precipitation, and length of time after a precipitation event and when an aircraft strike occurred. The types and frequency of bird strikes depend highly on the region and species struck. The Poisson regression revealed Cathartiformes as significantly struck by the DAF aircraft more often and can be predicted based on agricultural zone and precipitation. A binary regression did not demonstrate …

Relative Adrenal Insufficiency Is A Risk Factor And Endotype Of Sepsis - A Proof-Of- Concept Study To Support A Precision Medicine Approach To Guide Glucocorticoid Therapy For Sepsis, Chia-Hua Wu, Ling Guo, Dan Hao, Qian Wang, Xiang Ye, Misa Ito, Bin Huang, Chieko Mineo, Philip W. Shaul, Xiang-An Li

Saha Cardiovascular Research Center Faculty Publications

Introduction: 25-60% of septic patients experience relative adrenal insufficiency (RAI) and glucocorticoid (GC) is frequently used in septic patients. However, the efficacy of GC therapy and whether GC therapy should be based on the status of RAI are highly controversial. Our poor understanding about the pathogenesis of RAI and a lack of RAI animal model present significant barriers to address these critical issues.

Methods: Scavenger receptor BI (SR-BI) regulates stress-induced GC (iGC) production in response to stress. We generated SF1CreSR-BIfl/fl mice and utilized the mice as a RAI model to elucidate the pathogenesis of RAI and GC therapy in sepsis. …

Utilizing Precision Technologies To Validate A Real-Time Location System For Dairy Cattle And Monitor Calf Behaviors During Heat Stress, Emily Rice

Theses and Dissertations--Animal and Food Sciences

With the increase in on-farm precision dairy technologies (PDT) utilization, large quantities of information are readily available to producers. A more recently available technology for use in livestock species is the real-time location system. These technologies offer dairy producers the opportunity to monitor and track real-time locations of cows, track locomotion patterns, and summarize specific area usage. However, the usefulness of these insights is heavily dependent on the performance of the technology. Therefore, the first objective of this dissertation was to assess the positioning recording performance and the usefulness of the data recorded of a real-time location system (Smartbow GmbH; …

Effects Of Biosynthesized Bromoform On Enteric Methane Production, Animal Performance And Tissue Residues In Cattle, Sydney Davis

Theses and Dissertations--Animal and Food Sciences

Greenhouse gas (GHG) emissions have been implicated in global warming and extreme climate change conditions. The Environmental Protection Agency (EPA) has identified agriculture, more specifically cattle production, as a major contributor to global GHG production. When expressed as liters of gas, CO2 is considered the primary GHG produced, however, in terms of global warming potential (GWP), CH4 has 25-times greater potential than that of CO2. Due to the elevated GWP of CH4, we have constructed a series of experiments to investigate the use of a halogenated CH4 analogue derived from kelp (i.e., bromoform) as an inhibitor of enteric CH4 production …

Swosu Research And Scholarly Activity Fair 2023, Swosu Office Of Sponsored Programs

SWOSU Research and Scholarly Activity Fair Programs

On behalf of the members of the University Research and Scholarly Activity Committee (USRAC) and the Office of Sponsored Programs (OSP) at Southwestern Oklahoma State University (SWOSU) - Welcome to the Thirtieth SWOSU Research and Scholarly Activity Fair! There are 77poster presentations and 2 oral presentations involving over 130 student and faculty researchers, writers, presenters, artists, collaborators, and faculty sponsors encompassing activities from the SWOSU College of Pharmacy; SWOSU School of Nursing & Allied Health Sciences; and SWOSU Departments of Allied Health Sciences; Art, Communication & Theatre; Biological Sciences; Business; Computer Science; Chemistry & Physics; Engineering Technology; Language & Literature; …

Microglia Proliferation Underlies Synaptic Dysfunction In The Prefrontal Cortex: Implications For The Pathogenesis Of Hiv-1- Associated Neurocognitive And Affective Alterations, Hailong Li, Kristen A. Mclaurin, Charles F. Mactutus, Rosemarie M. Booze

Neuroscience Faculty Publications

Microglia, which are productively infected by HIV-1, are critical for brain development and maturation, as well as synaptic plasticity. The pathophysiology of HIV-infected microglia and their role in the pathogenesis of HIV-1-associated neurocognitive and affective alterations, however, remains understudied. Three complementary aims were undertaken to critically address this knowledge gap. First, the expression of HIV-1 mRNA in the dorsolateral prefrontal cortex of postmortem HIV-1 seropositive individuals with HAND was investigated. Utilization of a combined RNAscope multiplex fluorescent and immunostaining assay revealed prominent HIV-1 mRNA in microglia of postmortem HIV-1 seropositive individuals with HAND. Second, measures of microglia proliferation and neuronal …

Effects Of Translocation On Eastern Black-Tailed Rattlesnakes (Crotalus Ornatus) In The Northern Chihuahuan Desert, Jacob A. Coffman

Lewis Honors College Thesis Collection

AS HUMANITY continues to grow and expand, we inevitably encroach on wildlife habitat. This can create conflict between humans and wildlife. Human wildlife conflict usually occurs when humans are near areas where wildlife typically reside. A prime example is a farmer coming into conflict with animals that predate their seeds or livestock. The most common response to this problem in many areas is lethal control to prevent economic loss (Dickman 2010). This seems like an immediate resolution of the conflict in the human’s perspective, but this action can have many adverse effects. This killing may result in negative social outcry, …

Bibliography, Bruce A. Schulte

Faculty/Staff Personal Papers

Bibliography of publications by Bruce Schulte.

Establishing A Biochemical System For The Purification And Atpase Activity Of Gst-Dbp5, Sarah Utley, Rachel E. Rigsby Phd, Rebecca L. Adams Phd

Science University Research Symposium (SURS)

The export of mRNA out of the nucleus is a crucial step for eukaryotic gene expression. The export of mRNA transcripts is aided by Mex67, which allows export through the nuclear pore complex doorways in the nuclear envelope. Once out of the nucleus, a protein known as Dbp5, bound to ATP, Gle1, and Nup42 aids in the directionality of mRNA export by helping remove Mex67 from the mRNA strand. Following interaction with RNA, Dbp5 then hydrolyzes ATP so that it unbinds the mRNA, allowing for enzyme recycling. The goal of this study is to explore the ATPase activity of Saccharomyces …

Detecting Linc Complex Mps3 And Nuclear Pore Complex Ndj1 Protein Interactions On Yeast Nuclear Membrane S Through Fluorescence Microscopy, Dean Boecher, Rebecca Adams

Science University Research Symposium (SURS)

Though cancer cells have been shown to have abnormal nuclear morphologies and responses to mechanical forces, the mechanisms of how mechanical stress is translated into cellular action and structural reorganization within the nuclear envelope are largely unexplored. The Linker of Nucleoskeleton and Cytoskeleton (LINC) complex is a transmembrane protein complex that connects the actin cytoskeleton to the lamin nucleoskeleton, enabling mechanical forces to be translated between the cytoplasm and the nucleus. In cells exposed to physical stress, nuclear pore complexes (NPCs) –which control the exchange of biochemical signals and macromolecules in and out of the nucleus through mRNA export –have …

Analyzing Mex67 Interaction With The Ssa4 Transcript For Selective Export, Gretchen E. Stalnaker, Rebecca Adams

Science University Research Symposium (SURS)

In eukaryotic cells, such as the budding yeast Saccharomyces cerevisiae, mRNA export is the essential process in which mature mRNA is transported from its site of production, the nucleus, through the nuclear pore complex (NPC) to the cytoplasm, where it can then be translated into protein. This is accomplished when a transcript interacts with the mRNA export protein Mex67, which shuttles the message across the NPC. When the cell is under conditions of stress, such as heat shock, most mRNA export is prohibited, allowing for selective gene expression that prioritizes cell recovery. Specifically, SSA4, which encodes a protein that …

Development Of A Checklist To Assess University Campuses For Bird-Friendliness Qualities, Fatema Al-Hemoud, Chaze Espleta, Darlene Panvini

Science University Research Symposium (SURS)

Nashville, Tennessee recently became part of the U.S. Fish and Wildlife Service’s Urban Bird Treaty (UBT) program. The Nashville UBT aims to protect and enhance bird-friendly habitats, reduce hazards to birds, and connect people to nature through bird-related activities. To support these aims, students in a conservation biology course researched potential hazards and ideal habitats for birds on college campuses. They developed a checklist covering various bird-friendly criteria, including landscaping, water sources, windows, lighting, use of pesticides, and presence of cats and other bird predators. Students then beta-tested the checklist on the campus at Belmont University. The goals of this …

Assessing The Roles Of Myc And Egr1 In Endoderm Differentiation, Sadaf R. Shahebrahimi, Joseph Nguyen, Kyra Skye Thomas, Erick Spears

Science University Research Symposium (SURS)

The Wnt signaling pathway is evolutionarily conserved from fruit flies to humans. Strongly associated with development and embryonic morphogenesis, it is known to be required for early endoderm and later hindgut development in mammals. In adults, the Wnt signaling pathway is required for the proper maintenance of the intestinal epithelium and mutations in critical Wnt signaling pathway components are initiating events in the development of essentially all colorectal cancers. An important target gene whose expression is upregulated by Wnt signaling pathway activation is the oncogene MYC. This gene produces a transcription factor whose typical cellular role is to stimulate …

Testing Ssa4:Ade3 Reporters For Mcs Screening, Karah Edmonds, Rebecca Adams

Science University Research Symposium (SURS)

In eukaryotic cells, after transcription, mRNA is escorted from the nucleus to the cytoplasm to be translated. This process, called mRNA export, is essential for gene expression. However, when the cell exists in stressful conditions, mRNA export becomes regulated, and only select transcripts, including the stress-responsive SSA4 mRNA, can be exported. This project aims to uncover the mechanism of selective SSA4 mRNA export by generating a reporter that enables phenotypically visible expression under stressful conditions. Specifically, the ADE3 ORF was placed under the regulatory sequence of SSA4, which was anticipated to induce a red color for colonies only following stress. …

Evaluating Benthic Macroinvertebrate Populations In Response To Scouring Events In The Trinity River, Ca, Liam Hay, Michael W. O'Neil, Chloe Pieper-Wasem

Environmental Science & Management Senior Capstones

River systems across California have been impacted by appurtenant structures such as dams and diversions. These structures have had an adverse impact on Benthic invertebrate (BMI) communities by regulating river systems and changing the natural hydraulic pulses that follow seasonal precipitation. Benthic invertebrates are a critical food resource for salmonids and serve as an indicator of ecosystem health. Our study was interested in seeing the effects of scouring events on BMI in the Trinity River of Trinity County, C.A. Following a large precipitation event that occurred in the region in December 2022, an influx of water entered the river through …

Belowground Plant Communities: Seed Bank Dynamics And Relationship To Aboveground Vegetation In Grazing Allotment Of Klamath County, Oregon, Dellaina C. Morse, Scott M. Estepa

Environmental Science & Management Senior Capstones

Seed banks are valuable resources for restoration but are understudied. The species present in the aboveground composition influence the seed bank through seed inputs. Land managers utilize herbicides to selectively reduce certain species in the above- and belowground composition. The aim of this paper is to observe and assess the species present in a seed bank post-herbicide application, and to discuss the seed bank potential for future restoration at the specific site in Klamath County, Oregon. Soil core samples were collected in the field and brought to the lab where they were processed and prepared for germination. We watered and …

Artificial Light At Night Disrupts Male Dominance Relationships And Reproductive Success In A Model Fish Species, Lauren E. Closs, Muhammad Rahmad Rayan, Amin Sayyari, Ian Mayer, Finn-Arne Weltzien, Dianne M. Baker, Romain Fontaine

Biological Sciences Research

Environmental light is perceived and anticipated by organisms to synchronize their biological cycles. Therefore, artificial light at night (ALAN) disrupts both diurnal and seasonal biological rhythms. Reproduction is a complex physiological process involving integration of environmental signals by the brain, and release of endocrine signals by the pituitary that regulate gametogenesis and spawning. In addition, males from many species form a dominance hierarchy that, through a combination of aggressive and protective behavior, influences their reproductive success. In this study, we investigated the effect of ALAN and continuous daylight on the behavior and fitness of male fish within a dominance hierarchy …

Entangled Mangrove Roots: The Shrimp Industry, Ancestral Afro-Descendant People, And Community Resistance In Esmeraldas, Ecuador, O'Philia Le

Pitzer Senior Theses

Mangroves are one of the most important ecosystems because of the many services they provide on a local and global scale, but in contrast, are one of the most threatened by anthropogenic activities at a global level. Being sources of food for various kinds of fish, crustaceans, and mollusks, they are essential for the economy, culture, and livelihood of locals in Esmeraldas, Ecuador. This thesis takes an environmental justice approach in the discussion of the loss of mangroves in Esmeraldas, Ecuador. While toxic industries may not be apparent at first, environmental injustice prevails in adverse human health effects, environmental degradation, …

Neuroimmune-Mediated Alteration Of Acute Alcohol Exposure In The Mesolimbic Dopamine System, William T. Harris, P. E. Williams, Scott C. Steffensen

Library/Life Sciences Undergraduate Poster Competition 2023

2nd place open house

Dopamine (DA) transmission is a key player in the rewarding aspects of ethanol (EtOH) as well as EtOH dependence.

The current dogma is that DA transmission is increased during acute EtOH exposure via the inhibition of ventral tegmental area (VTA) GABA neurons and that the excitation of VTA GABA neurons during withdrawal results in decreased DA transmission.

Much recent evidence indicates that neuroimmune interactions may mediate this process. •

Microglia, the major neuroimmune effector in the brain, may be a key mediator in this process by releasing cytokines following activation.

Macrophages, a major immune effector in …

Estimation Of Breeding Values Using Different Densities Of Snp To Inform Kinship In Broiler Chickens, Mayara Salvian, Gabriel Costa Monteiro Moreira, Ândrea Plotzki Reis, Brayan Dias Dauria, Fabrício Pilonetto, Izally Carvalho Gervásio, Mônica Corrêa Ledur, Luiz Lehmann Coutinho, Matthew L. Spangler, Gerson Barreto Mourao

Department of Animal Science: Faculty Publications

Background: Traditionally, breeding values are estimated based on phenotypic and pedigree information using the numerator relationship (A) matrix. With the availability of genomic information, genome-wide markers can be included in the estimation of breeding values through genomic kinship. However, the density of genomic information used can impact the cost of implementation. The aim of this study was to compare the rank, accuracy, and bias of estimated breeding values (EBV) for organs [heart (HRT), liver (LIV), gizzard (GIZ), lungs (LUN)] and carcass [breast (BRST), drumstick (DRM) and thigh (THG)] weight traits in a broiler population using pedigree-based BLUP (PBLUP) and …

Effect Of Increasing Corn Silage Inclusion In Finishing Diets Cattle With Or Without Tylosin On Performance And Liver Abscesses, Hannah C. Wilson, Levi J. Mcphillips, Bradley M. Boyd, Andrea K. Watson, Jim C. Macdonald, Galen E. Erickson

Department of Animal Science: Faculty Publications

A pooled analysis was performed to evaluate whether corn silage fed at 15% or 45% of diet DM impacted liver abscesses prevalence at slaughter in five previous experiments. Cattle fed 15% corn silage had 7.8% abscessed livers compared to 4.1% for cattle fed 45% corn silage when all diets contained tylosin. While improved due to increased corn silage inclusion, the objective of the current finishing study was to determine the impact of silage inclusion in finishing diets with and without tylosin on performance and incidence of abscessed livers in beef cattle. A total of 640 (BW = 334 ± 25 …